4.

RESULT

Time (minute)

Fish A Mouth Movement (open) Isolation Exposure (10min) (20min) 1st 2nd 10min 10min Opercular Cover (Gills) Isolation Exposure (10min) (20min) 1st 2nd 10min 10min R/S I/D R/S I/D R/S I/D S D R I R I S D R I R I S D S D S D S D S D S D S D R I R I S D R I S D S D S D R I S D S D S D S D S D R I S D S D R I Mouth Movement (open) Isolation Exposure (10min) (20min) 1st 2nd 10min 10min

Fish B Opercular Cover (Gills) Isolation Exposure (10min) (20min) 1st 2nd 10min 10min R/S I/D R/S I/D R/S I/D S D R I R I S D R I R I S D S D S D S D S D S D S D R I R I S D S D S D S D R I R I S D R I S D S D R I R I S D S D R I

1 2 3 4 5 6 7 8 9 10

Table 1: Mouth movement and Opercular covers movement observed in two male Siamese fighting fish, Beta splendens in two different conditions: Isolation and Exposure to each other. R/S: Rapid/Slow movement; I/D: Increase/Decrease in frequency for opercular cover movements (gills) and ticked, refers to opening of the mouth, nil refer to closing of the mouth.