Reg. no. - Reg. no. -R eg. no. 2010-11 ChemistryInvestigatory Project Report M.

Thamarai selvi XII A Topic: To determine the amount of casein present in different samples of milk. ( AFFILIATED TO THE CENTRAL BOARD OF SECONDARY EDUCATION )

Laboratory certificate This is to certify that _______________has satisfactorily completed the courseof CHEMISTRY INVESTIGATORYPROJECTprescribed by the CBSE undertheAISSCEcourse in th e laboratory of this school in the year2010-2011. Signature of the external examiner:Signature of Principal:Signature of teacher i n-charge:

Acknowledgement I sincerely and profusely thankMr.Manjunath Reddy(Chemistry teacher)for his valu able guidance, advice andfor giving useful suggestions andrelevant ideas that fa cilitated an easyand early completion of this project.I also express my deep gra titude toMr.Suresh(lab assistant), who wasinstrumental in this regard by providi ngall the necessary materials required inthis project.

Index Contents y Aim y D escription y Requirements y Procedure y Observation

y Inference y Bibliography

Aim To determine the amount of casein present in differentsamples of milk.

Description Casein is the main protein constituent of milk. Itconstitutes about 80% of the t otal protein in cow smilk and about 3% of its weight. It group of proteinprecipita ted when the milk is slightly acidified. Itdissolves slightly in water, extensiv ely in alkalis orstrong acids. Casein is a complete protein meaningthat it contai ns all of the essential amino acids, whichthe body can not manufacture on its ow n. Whendried, it is a white, amorphous powder without tasteand odour. It is a mi xed phosphoprotein and occurs inmilk as calcium salt (calcium caseinate) in the form of micelle. The micelle has a negative charge. When anacid is added to the milk, the negative charges areneutralized. Calcium caseinate + acetic acid casein (s)+ calcium acetate (aq)

Requirements Apparatus and Chemicals y 250 ml beakers y F unnel y Glass rod y Chemical balances y Test tubes y F iltration flask y Bunsen burner y D ifferent samples of milk y 1% acetic acid solution y Saturated ammonium sulphate solution

P rocedure y Take a clean dry beaker, put into it 20cc of cow s milk and add 20 ml of saturated ammonium sulphate solution slowly andwith stirring. F at along with casein willprecipitate out. y F ilter the solution and transfer theprecipitates in another beaker. y Add about 30 ml of water to theprecipitate. y Only casein dissolves in water formingmilky solution leaving fat undissolved. y Heat the milky solution to about 40 C andadd 1% acetic acid solution drop wise,wh en casein gets precipitated.

y F ilter the precipitate, wash with waterand let the precipitate dry. y Weigh the dry solid mass in a previouslyweighed watch glass. y Repeat the experiment with othersamples of milk.

Observation table Volume of milk taken in each case = 20 ml.Name of the milk Weight of casein % of caseinCow milk 0.68 g 3.4Buffalo milk 0.84 g 4.2Goat milk 0.66 g 3.3Sheep milk 1.10 g 5.5

Inference D ifferent samples of milkcontain differentpercentage of casein.

Bibliography y www.google.com y Comprehensive prac tical chemistry y www.sciencebuddies.org

G allery Preparationof saturated ammonium sulphate sol. D

ifferentsamples of milk.

Cow s milk ( 20 cc ).Addition of saturated ammonium sulphate solution

Proteinsand fat precipitate out. F iltrationof the solution.

Separating out the filtrate.Close up view of the filtrate.

Heating thefiltrate with 30ml water at 40 C with1% acetic acid solution.Caseinprot ein separate out.

Caseinprotein washed and separated out andlet to dry.After drying.

Casein protein from differentsamples of milk. Goat s milk Cow s milk Buffalo s milk Sheep s milk

More From This User 19 p. 37272987 Chemistry Investigatory Project Thamshak Andrew Babu 2679 Reads Related 14 p. CBSE Class XII Chemistry Investigation Project Work Raj 183407 Reads 13 p. AJCHEM Animesh Jain

939 Reads 2 p. ambika ambikamehrotra 701 Reads Next 11 p. ABHI 07 Abhishek N Rokhade 444 Reads 9 p. CHEM PROJECT Swayams Mishra 1503 Reads 2 p. Chemistry Investigatory Project ( Errors) Amulya Murthy 747 Reads Previous | Next 1 p. Buy Order Allergenic Extract, Casein, Cow and Goat Milk Online Chea... hqdoc 71 Reads 10 p. Milk is a white liquid secreted by the mammary gland of female cows Akshay Kheral 342 Reads 4 p. p1 upadhayaymadan 65 Reads Previous | Next 13 p. Casein bhawnanarang 236 Reads 13 p. Casein Product ka6_jeram 190 Reads 13 p. Process1 Abhishek Kapoor 146 Reads Previous | Next 43 p. Neurological disorders Lalitha Chelliah 475 Reads 43 p. 7. Appendix 1 Lalitha Chelliah 475 Reads 43 p. A2 Milk Report Lalitha Chelliah 475 Reads Previous | Next 7 p. Dairy

hemali_2986 639 Reads 4 p. Project Rajat Parmar 46 Reads 2 p. Casein Production Moseyspeed 426 Reads Previous | Next 14 p. Milk, An Excerpt from American Farmstead Cheese Chelsea Green Publishing 1020 Reads 10 p. Sodium Caseinate Calcium Caseinatate Protein Gujarat India Charotar Casein Company Gujarat 188 Reads 10 p. Charotar Casein Company Gujarat India IndiaMARTSuppliers 154 Reads Previous Leave a Comment Comment must not be empty. You must be logged in to leave a comment. Submit Characters: 400 Priyanka Ramawat oh god ye to download hi nai ho raha hai? reply01 / 28 / 2011 Thunder Karma chalo bhai 1 to download hua.................... reply12 / 22 / 2010 Surjeet Sarkar good knowledge of this project are prepared.It;s a so complicated & so nice. ?Th anks for this project are prepared.Bcoz it help in all student. reply12 / 10 / 2010 Naren Mudgil kya baat ha reply10 / 20 / 2010 Comment must not be empty. You must be logged in to leave a comment. Submit Characters: ... 37272987 Chemistry Investigatory Project Thamshak Download or Print Add To A Collection 2.6K Reads 28

Readcasts 1 Embed Views This is a private document. Published by Andrew Babu Follow Search TIP Press Ctrl-F F to quickly search anywhere in the document. Search Search History: Searching... Result 00 of 00 00 results for result for p. Info and Rating Category: Uncategorized. Rating: Upload Date: 09/18/2010 Copyright: Attribution Non-commercial Tags: This document has no tags. Free download as PDF File (.pdf), Word Doc (.doc / .docx), text file (.txt) or r ead online for free. Flag document for inapproriate content