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Genetics
Genome (The sum total of genetic material of a cell is referred
to as the genome.)
Genotype Phenotype
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Chromosome
Procaryotic
Histonelike proteins condense DNA
Genes
Three categories
Structural Regulatory Encode for RNA
Eucaryotic
Histone proteins condense DNA
Genotype
Phenotype
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Flow of Genetics
DNA =>RNA=>Protein
Replication Transcription Translation
DNA
Structure Replication
DNA is lengthy and occupies a small part of the cell by coiling up into a smaller package.
Fig. 9.3 An Escherichia coli cell disrupted to release its DNA molecule.
Structure
Nucleotide
Phosphate Deoxyribose sugar Nitrogenous bases (purines- adenine, guanine;
pyramidines thymine, cytosine)
Purines and pyrimidines pair (A-T or G-C) and the sugars (backbone) are linked by a phosphate.
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Replication
Semiconservative Enzymes Leading strand Lagging strand
Okazaki fragments
Semiconservative
New strands are synthesized in 5 to 3 direction
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Semiconservative replication of DNA synthesizes a new strand of DNA from a template strand.
Enzymes
Helicase DNA polymerase III Primase DNA polymerase I Ligase Gyrase
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Leading strand
RNA primer initiates the 5 to 3 synthesis of DNA in continuous manner
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Lagging strand
Multiple Okazaki fragments are synthesized Okazaki fragments are ligated together to form one continuous strand
Fig. 9.6 The bacterial replicon: a model for DNA Synthesis
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Replication processes from other biological systems (plasmids, viruses) involve a rolling cycle.
RNA
Transcription
Message RNA (mRNA) Transfer RNA (tRNA) Ribosomal RNA (rRNA)
Codon
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Transcription
A single strand of RNA is transcribed from a template strand of DNA RNA polymerase catalyzes the reaction Synthesis in 5 to 3 direction
mRNA
Copy of a structural gene or genes of DNA
Can encode for multiple proteins on one message
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tRNA
Copy of specific regions of DNA Complimentary sequences form hairpin loops
Amino acid attachment site Anticodon
rRNA
Consist of two subunits (70S) A subunit is composed of rRNA and protein Participates in translation
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Ribosomes bind to the mRNA, enabling tRNAs to bind, followed by protein synthesis.
Codons
Triplet code that specifies a given amino acid Multiple codes for one amino acid 20 amino acids Start codon Stop codons
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Protein
Translation
Protein synthesis have the following participants
mRNA tRNA with attached amino acid Ribosome
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Translation
Ribosomes bind mRNA near the start codon (ex. AUG) tRNA anticodon with attached amino acid binds to the start codon Ribosomes move to the next codon, allowing a new tRNA to bind and add another amino acid Series of amino acids form peptide bonds Stop codon terminates translation
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For procaryotes, translation can occur at multiple sites on the mRNA while the message is still being transcribed.
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Regulation
Lactose operon
sugar
Repressible operon
Amino acids, nucleotides
Antimicrobials
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The regulation of sugar metabolism such as lactose involves repression in the absence of lactose, and induction when lactose is present.
The regulation of amino acids such as arginine involves repression when arginine accumulates, and no repression when arginine is being used.
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Antimicrobials
Ex. Antibiotics and drugs can inhibit the enzymes involved in transcription and translation
Mutations
Changes made to the DNA
Spontaneous random change Induced chemical, radiation. Point change a single base Nonsense change a normal codon into a stop codon Back-mutation mutation is reversed Frameshift reading frame of the mRNA changes
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Repair of mutations involves enzymes recognizing, removing, and replacing the bases.
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The Ames test is used to screen environmental and dietary chemicals for mutagenicity and carcinogenicity without using animal studies.
Effects of mutations
Positive effects for the cell
Allow cells to adapt
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Recombination
Sharing or recombining parts of their genome
Conjugation Transformation Transduction
Conjugation
Transfer of plasmid DNA from a F+ (F factor) cell to a F- cell An F+ bacterium possesses a pilus Pilus attaches to the recipient cell and creates pore for the transfer DNA High frequency recombination (Hfr) donors contain the F factor in the chromosome
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Transformation
Nonspecific acceptance of free DNA by the cell (ex. DNA fragments, plasmids) DNA can be inserted into the chromosome Competent cells readily accept DNA
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DNA released from a killed cell can be accepted by a live competent cell, expressing a new phenotype.
Transduction
Bacteriophage infect host cells Serve as the carrier of DNA from a donor cell to a recipient cell
Generalized Specialized
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Transposon
Jumping genes Exist in plasmids and chromosomes Contains genes that encode for enzymes that remove and reintegrate the transposon Small transposons are called insertion elements
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