THE DEVELOPMENT OF DNA

DATABASE FOR GONYSTYLUS

BANCANUS IN SARAWAK

BIBIAN DIWAY
NURUL FARHANA ZAKARIA
SULIANA CHARLES
KEVIN NG KIT SIONG
LUCY CHONG

Copyright 2010
Sarawak Forest Department
Sarawak Forestry Corporation

Sarawak Forest Department

Wisma Sumber Alam
Jalan Stadium, Petra Jaya
93050 Kuching, Sarawak, Malaysia.
Tel:
Fax:

+6(082) 319158
+6(082) 445639

Sarawak Forestry Corporation

Botanical Research Centre
Km 20, Jalan Puncak Borneo
93250 Kuching, Sarawak, Malaysia.
Tel (D/L):
+6( 082) 615888
Fax: +
+6 (082) 61953
E-mail: bibian@sarawakforestry.com
Web: www.sarawakforestry.com

TABLE OF CONTENTS

ii

LIST OF TABLES

iii

LIST OF FIGURES

iv

ACKNOWLEDGEMENT

EXECUTIVE SUMMARY

vi

1.0 INTRODUCTION

1.1.1

Protection of Ramin

1.1.2

Use of DNA for timber identification

2.0 OBJECTIVES

3.0 METHODOLOGY

3.1.1

Study site and samples collection

3.1.2

DNA Extraction

3.1.3

PCR amplification and fragment analysis

3.1.4

Data analysis

4.0 RESULTS AND DISCUSSIONS

4.1.1

Genetic Diversity

4.1.2

Populations differentiation

5.0 CONCLUSIONS

11

6.0 RECOMMENDATIONS

11

REFERENCES

12

PHOTOS

14

APPENDIX: Genotype Profile

19

ii

LIST OF TABLES

Table 1: Export destinations for Ramin products (January to November 2009) for
Sarawak.

Table 2: Export of Ramin from 2007 to 2009 (January to November).

Table 3: Surveyed populations and number of individuals sampled.

Table 4: Number of alleles per locus.

Table 5: Estimates of genetic diversity for 9 populations of ramin based on 18

microsatellite loci. N, number of individuals sampled from the population; PIC,
polymorphic information content; NA, number of alleles; MNA, mean number
of alleles; Ar, Allelic richness; Ho, observed heterozygosity; He, expected
heterozygosity; SD, standard deviation.
8
Table 6: Genetic diversity parameters (expected heterozygosities) of other tropical tree
species based on microsatellite studies.
8
Table 7: Estimates of pairwise Fst distance between the analysed populations.

Table 8: Genetic differentiation, as estimated by Fst distance among the analysed

populations.
10

iii

LIST OF FIGURES

Figure 1: Distribution of Peat Swamp Forest in Sarawak

Figure 2: Example of some fragment data showing variable alleles size from three
individuals of Gonystylus bancanus.

Figure 3: Dendrogram of genetic distance between populations based on UPGMA

generated using GDA

10

Figure 4: Cluster of population based on Structure ver 2.3. Population divided into
two subregion: Red and Blue. Population 1: Sedilu;2: Lingga; 3: Serapau;
4: Loagan Bunut; 5: Kayangeran;6: Maludam; 7: Manggut; 8: Betong and
9: Naman.
11

iv

ACKNOWLEDGEMENT

The authors would like to acknowledge Datu Len Talif Salleh, the Director of Sarawak Forest
Department cum CEO of Sarawak Forestry Corporation, Mr. Ngui Siew Kong, the Asistant
Director of Sarawak Forest Department and Mr. Nigel Lim, the General Manager of Sarawak
Forestry Corporation for permitting us to conduct this research. We would like to thank Dr. Lee
Soon Leong from FRIM, managers and colleagues from Applied Forest Science and Industrial
Development of Sarawak Forestry Corporation for their fruitful suggestions and comments. Not
forgetting all staff of Sarawak Forestry Corporation and Forest Research Institute Malaysia
involved in implementing the project. Special thanks also go to Mr Tsuyoshi Harata for assisting
in statistical analysis and Dr. Koichi Kamiya for editing the report, Miss Stephanie Ronny, Mr.
Mohamad Shahbudin Sabki and Mr.Azahari Omar for their assistant in this project, and Rantai
Jawa and Andy Samuel for their photos.
Last but not least we would like to thank ITTO-CITES for funding this project and NRE the
executing agencies.

EXECUTIVE SUMMARY

Gonystylus bancanus popularly known as ramin is an endemic species to Peat Swamp Forest.
High demand for the ramin products and depletion of habitat due to land conversion has put
ramin in the Appendix II CITES - listed species. Various protection measures has been taken by
Sarawak government including imposed on ban on export of ramin log and for export of ramin
products will need verification from Customs on the CITES Export Permit. However the control
measure by the CITES could not guarantee no illegal trading of ramin. Often the illegal trading
happened through false declaration on the species name and origin of the products. This project
is to develop the DNA database for Gonystylus bancanus in Sarawak to enhance the enhance
Sarawak and Malaysia government as well as CITES effort to combat illegal trading. It is an
early stage in the effort to use DNA for tracing and tracking of timber origin and verification of
species.
The plant materials consisted of leaves and barks samples were collected from nine ramin
populations throughout. For extraction of DNA several methods were used including plant
extraction kit however only cetyltrimethyl ammonium bromide (CTAB) method with
modification yielded a good quality of DNA. From a total of 25 microsatellite markers tested, 18
markers were selected to amplify the DNA and to generate alleles frequencies data for the
database. A total of sixty seven unique alleles were detected providing useful information for
tracing of timber origin. Genetic diversity measured was generally high for most populations
except for Kayangeran Forest Reserve and Loagan Bunut National Park. The expected
heterozygosity values ranged from 0.682 to 0.803 indicated that genetic diversity was generally
high. The level of genetic diversity of ramin in Sarawak populations was as high as level of
genetic diversity for other species of tropical forest.
The genetic identity based on Neis 1978 identity index, ranged from 0.62994 between
populations of Kayangeran and Serapau to 0.982971 between populations of Manggut and
Lingga. UPGMA cluster analysis using Neis 1978 genetic distance clustered the populations
into two clusters which positively related to geographical location. Sedilu, Lingga, Serapau,
Maludam, Manggut, Betong and Naman were clustered into one group and the isolated
populations Kayangeran and Loagan Bunut were clustered in another group. The closest genetic
distance was 0.022656 between populations of Manggut and Lingga whereas the furthest was
0.462131 between populations of Kayangeran and Serapau. The high value of Fst indicated the
ramin populations in Sarawak in former days were connected but now becoming fragmented.

vi

Unique alleles detected within populations and cluster analysis indicated the ability and
usefulness of microsatellite markers selected for tracking and tracing of ramin populations in
Sarawak. Thus they are effective for the development of DNA database. However for the high
accuracy and reliability of microsatellite markers for tracking and tracing timber origin and
species identification should base on many loci. In addition, similar project should be extended
to other ramin population in other states and neighboring countries in order to enhance
implementation of CITES legislation/regulations and the enforcement officials capacity to curb
illegal logging within this region.

vii

1.0 Introduction
Gonystylus bancanus locally know as ramin telur belongs to the family Thymelaeaceae is a
native species of fragile Peat Swamp Forest (PSF). The wood is creamy yellow in colour with
moderately fine and even texture, is easy to saw, bore and turn and has smooth planned surface.
It is one of the most valuable tropical hardwoods and highly sought after for decorative cabinet
timber, furniture, squash court flooring and interior decorative work (Lim et al., 1999).
The largest importer of ramin sawn timber and products are countries such as China including
Hong Kong, United States and Japan (Table 1). From January to November 2009 the production
and export of Ramin for Sarawak reported were 2,591 m3, a decline compared to production in
2008, 2007 and 2006 (Table 2). The decline in ramin production for 2009 as reported by
Malaysia during the Fifty-ninth CITES meeting at Doha (Qatar) on 12 March 2010 was the result
of global economic recession faced by the timber sector since late 2008.

Table 1 Export destinations for Ramin products (January to November 2009) for Sarawak
Export destination
Market
China (including Hong Kong)
USA
Japan
Others
Total

Volume (m3)
623
454
448
1,066*
2,591

Source: CITES, 2010

Ramin products
Products
Sawn Timber

Volume (m3)
1,153

Dowels/Mouldings

1,438

Total

2,591

Table 2 Export of Ramin from 2007 to 2009 (January to November)

Export Quota (m3)
Export volume (m3)
Export performance (%)

Source: CITES 2010

2006
22,000
12,161

2007
12,875
10,434.31
81.04

2008
3,178
3,063.09 (5,112.1214m3)**
96.38

2009
3,178
2,591
81.53

** including stockpile of year 2007

The reduction in ramin production over the years was also the result of decline in areas covered
by peat swamp forest. Based on the 1995/1996 satellite images, 9.2 % or 1.13 million ha of
Sarawaks land area was covered by Peat Swamp Forest. However, in 2002, the Landsat and
SPOT data showed that 98.5 % of the peat swamp forest has been opened up for various
purposes leaving only 18,920.45 ha relatively untouched PSF (Wong 2002). From the total area
1

opened up, 78,260.96 ha has been converted into plantation area. The total area converted to
plantation was further increased to 389,483 ha in 2004 (Figure provided by Sarawak Forestry
Department GIS unit and reported by Lee 2005).
Due to the reduction in PSF within PFE and the decline in ramin production, Malaysia
particularly Sarawak has been reported by Environmental Investigation Agency (EIA) and
Telapak of being involved in smuggling and laundering of illegal Indonesian ramin into
number of importing countries, including US and UK (Traffic 2004, EIA/Telapak 2004). Worse
still, Malaysia has also been accused of failing to carry out a proper non-detriment finding for
ramin export and has no effective control in place on ramin harvesting (EIA/Telapak 2006). The
Malaysian government through Malaysia Timber Council (MTC, 2004) responded that the
country does not condone any illegal trade in timber and has implemented various measures to
combat the entry of illegal logs.

1.1 Protection of Ramin

Current protection measures taken by Sarawak Government are in accordance with Section 96
(1) of Forest Ordinance 1954 including the ban on export of ramin logs that was enforced in
1980, ban on export of ramin shorts and squares and restriction of export of ramin timber
implemented in 1991. At the last points of export, exporters also need to get verification by
Customs on the CITES Export Permit (CITES, 2010).
However, there may be cases in which traders circumvent CITES controls by providing false
declaration of the species involved. Inspection of timbers is currently done based on wood
anatomy which identifies timber into group and is generally not identified to species level and
source of timber is not determined. In the case of ramin, the wood anatomy is very similar with
Alstonia, Antiaris spp., Brosimim aliscastrum, Dyera costulata, Endospermum spp.,
Chrysophyllum beguei, Jacaranda copaia, Neolamarckia cadamba, Pterygota spp., Simarouba
amara and Terminalia superba.

1.2 Use of DNA for timber identification

The application of DNA profiling in human forensic and paternity proof has long been used. The
same technique has also been applied in the identification of animals (Butler, 2005). Recently in
Peninsular Malaysia, a study using chloroplast DNA proved that the geographical origin of
Neobalanocarpus heimii can be traced (Tnah et al., 2009), and the comprehensive DNA profiling

database developed for N. heimii can serve for the random match probability estimation of an
illegal logging with its original stumps (Tnah et al., 2010).

2.0 Objective
The aims of the study are; (i) to develop a DNA database as initial stage to enhance efforts of
tracing and tracking of Gonystylus bancanus timber, (ii) to estimate the genetic diversity of
ramin populations in Sarawak, and (iii) to determine extents of genetic differentiation among
populations that contribute to baseline information for the tracing and tracking system using
DNA markers.

3.0 Methodology
3.1 Study sites and samples collection
Samples were collected from known geographical range of peat swamp forest in Sarawak (Fig.
1). Peat swamp areas in the central part of Sarawak were not surveyed for this project because
the areas are earmarked for further development. A total of nine populations were sampled
(Table 3). At least 30 individuals were collected from each population, though few areas
involved intensive search due to limited stand density remaining.

Figure 1 Distribution of Peat Swamp Forest in Sarawak

Kayangeran

Loagan
Bunut

Sedilu
Lingga
Serapau
Manggut
Maludam
Betong

Earmarked for development

Naman

Table 3 Surveyed populations and number of individuals sampled.

Population
number

Locality

Nearest
town

Latitude

Longitude

1
2
3
4

Sedilu
Lingga
Serapau
Loagan Bunut

Simunjan
Sri Aman
Sri Aman
Miri

N01022'
N01021'
N01019'
N03047'

E110049'
E111010'
E111012'
E114014'

No
of
individuals
sampled
31
30
30
101

5
6
7
8

Kayangeran
Maludam
Manggut
Betong

Lawas
Sebuyau
Betong
Betong

N4o53
N01o38
N01o29
N01o 25

E115o25
E111o 03
E111o 16
E111o 23

29
36
53
40

Naman

Sibu

N02o11

E111o 50E

55

3.2 DNA extraction

Genomic DNA was extracted using the prescribed method of Murray and Thompson (1980) with
modification. 0.5 g of leaf or inner bark tissues were mixed with 5ml of preheated (65 oC) CTAB
extraction buffer (2% CTAB, 20mM EDTA, 100mM Tris-HCl, 4M NaCl, 1% PVP, 2% mercaptoethanol) in 15 ml tube and incubated at 50oC for 30 min. After incubation, equal
amount of chloroform-isoamyl alcohol (24:1) was added and mixed gently. The mixture was
centrifuged at 3500 rpm for 10 min. The aqueous layer was transferred into new 15 ml tube and
the DNA was precipitated with 2/3 volumes of isopropanol. The mixture was pelleted by
centrifuge at 3500 rpm for 5 minutes. Subsequently, the supernatant was removed and the DNA
pellet was washed with 76% ethanol, left to dry and the DNA was redissolved in TE buffer. The
DNA was further purified using High Pure PCR Template Preparation Kit (Roche Diagnostics).

3.3 PCR amplification and fragment analysis

PCR amplification was performed using Qiagen multiplex PCR in 8.0 l reaction mixture,
consisting of 2x Type-it multiplex PCR master kit. Eighteen microsatellite markers were utilized.
The multiplex PCR was amplified using a GeneAmp PCR System 9700 (Applied Biosystem) at
95oC for 5 minutes initial denaturation, 35 cycles of 95 oC for 30 seconds of denaturation, 50 to
57oC for 1 min 30 seconds annealing, 72oC for 30 seconds of extension, followed by final
extension at 60oC for 30 minutes. The amplification product (1 L PCR product) was combined
with 9.9 L of Hi-Di formamide (Applied Biosystems) and 0.1 L of 400 HD Rox (Applied
Biosystems) to form master mix for fragment analysis. The master mix was assigned against ABI
3130x1 Genetic Analyzer System. The fragment data was edited and genotype profile created
using GeneMapper TM v3.7 software (Applied Biosystems).

3.4 Statistical Analysis

Population variation within and among population was quantified by calculating the allele
frequencies, mean number of alleles (MNA) per locus, observed heterozygosity (Ho) and
expected heterozygosity (He) and polymorphic information content (PIC) with The Excel
Microsatellite Toolkit software computer program (Park, 2001). A number of polymorphic loci
and allelic diversity have been shown to be reliable indicators of a genetic variation and can
show the effect of bottlenecks and genetic drift (Nei, 1978).

FSTAT, ver. 2.9.3.2 (Goudet,2002) was used to calculate the allelic richness, Ar (Petit et al.,
1998), Wrights (1951) F statistics (Fst, Fis and Fit) based on Weir and Cockerhams (1984) to
determine the distribution of genetic variation within and among populations and R-statistics,
Rst, (Slatkin,1995; Goodman,1997) calculation, to estimate a population differentiation. Fit and
Fis measure heterozygosity of individual relative to the total population and its subpopulations,
respectively; Fst is a measure of genetic differentiation among populations.
Cluster analysis based on genetic distances estimated from Neis (1978) index of similarity was
performed using Genetic Data Analysis (GDA) program (Lewis and Zaykin, 2001). The
population dendrograms were performed with the tree drawing software TreeView (Page, 2001).

4.0 Results and Discussions

4.1 Genetic diversity
Table 4 Number of alleles per locus
Marker

Sedilu

Lingga

Serapau

Gba082
Gba092
Gba100
Gba108
Gba129
Gba147
Gba175
Gba348
Gba430
WGb17
WGb22
WGb24
WGb29
WGb32
WGb37
WGb38
WGB39
Total

19
9
11
21
14
16
2
7
13
13
12
9
12
19
6
7
8
207

20
11
6
22
15
17
2
7
13
14
11
10
15
17
6
6
8
206

21
9
9
19
14
16
3
11
11
14
11
7
14
15
6
7
6
200

Loagan
Bunut
16
7
5
14
11
16
2
10
11
16
6
10
9
16
5
5
4
168

Kayangeran

Maludam

Manggut

Betong

Naman

Total

8
4
4
8
10
14
2
6
7
7
2
7
4
10
6
3
4
109

20
9
10
19
12
20
2
9
11
15
10
10
14
16
6
7
6
202

22
11
10
21
15
24
2
11
11
17
14
11
15
20
7
6
6
227

23
7
10
24
16
20
2
11
12
15
11
10
19
17
7
6
7
222

24
9
12
21
17
21
3
9
18
17
11
9
17
20
7
9
8
239

32
15
18
35
25
37
3
17
20
24
15
17
27
28
9
11
11
356

A total of 356 alleles were detected from 18 polymorphic loci (Table 4). The average number of
alleles and allelic richness was relatively high for most populations except for Kayangeran (6.06
and 5.95, respectively) and Loagan Bunut (9.33 and 7.38, respectively) (Table 5). The average
number of alleles over all populations was 10.99. Figure 2 shows examples of some observed
alleles derived using GeneMapper ver 3.7 software. Polymorphic information content (PIC) was
the lowest at Kayangeran (0.621) and the highest at Sedilu (0.76) (Table 5).
6

Sixty seven of the 356 alleles detected occurred only in one of each of the population. Sedilu had
13 unique alleles that were not detected in other populations, Naman and Manggut had 9 unique
alleles, while Lingga and Betong each had 8 unique alleles. Other populations had less than 7
unique alleles. These unique alleles are very informative used for population identification.

Figure 2 Example of some fragment data showing variable alleles size from three
individuals of Gonystylus bancanus.

Observed and expected heterozygosities, Ho and He were generally high for all populations with
no value less than 0.6. When all populations were compared together, Kayangeran and Loagan
Bunut had the lowest value observed. On average, the expected heterozygosity was 0.766, which
was within the range observed for other tropical species (Table 6). However, the values of Fis
were significantly different from zero for three populations, an indication of inbreeding within
population (Table 5).
The value of measured genetic diversity at Kayangeran and Loagan Bunut are low, but this may
be a function of sampling error. Because the trees at both sites grow in a clump, most of the
samples were collected from trees less than 10 meters apart from one to another. Sedilu,
Manggut and Naman had high value of genetic diversity but these populations had significant
departure from Hardy Weinberg Expectation, with a deficiency of heterozygotes.

Table 5 Estimates of genetic diversities for 9 populations of ramin based on 18

microsatellite loci. N, number of individuals sampled from the population; PIC,
polymorphic information content; MNA, mean number of alleles; Ar, Allelic
richness; Ho, observed heterozygosity; He, expected heterozygosity; SD, standard
deviation; Fis, Fixation index.
Pop

PIC

MNA

Ar

Ho(SD)

He(SD)

Fis

Sedilu
Lingga
Serapau
Loagan Bunut
Kayangeran
Maludam
Manggut
Betong
Naman
Mean

31
30
30
101
29
36
53
40
55

0.762
0.732
0.738
0.677
0.621
0746
0.746
0.748
0.761
0.725

11.50
11.44
11.11
9.33
6.06
11.22
12.61
12.33
13.28
10.99

10.96
10.8
10.66
7.38
5.95
10.34
10.33
10.67
10.96
9.78

0.688(0.019)
0.738(0.019)
0.747(0.019)
0.706(0.011)
0.655(0.021)
0.748(0.017)
0.731(0.014)
0.745(0.016)
0.752(0.014)
0.723(0.016)

0.803(0.036)
0.770(0.039)
0.775(0.036)
0.719(0.032)
0.682(0.036)
0.785(0.030)
0.782(0.034)
0.787(0.030)
0.792(0.030)
0.766(0.033)

0.145*
0.042
0.037
0.018
0.039
0.048
0.065*
0.053
0.052*
0.056

*Significantly different from zero (P<0.05).

Table 6 Genetic diversity parameters (expected heterozygosities) of other tropical tree

species based on microsatellite studies.
Species
Shorea curtisii
Shorea leprosula
Shorea ovalis
Neobalanocarpus heimii
Dryobalanops aromatic
Shorea macrophylla

No of
samples

No of
Loci

40
173
156
30
90
32

8
7
7
4
7
2

No of
populations
1
1
1
1
5
1

He
0.64
0.70
0.64
0.78
0.71
0.75

Reference
Ujino et al., 1998
Ng et al., 2004.
Ng et al., 2004
Konuma et al., 2000
Lim et al., 2002
Konishi et al., 2004

4.2 Population differentiation

Table 7 Neis (1978) genetic identity is above the diagonal, and Neis (1978) genetic distance
coefficients are below the diagonal
Sedilu

Lingga

Serapau

Kayangeran

Maludam

Manggut

Betong

Naman

0.912005

Loagan
Bunut
0.761285

Sedilu

0.921376

0.716141

0.926301

0.903312

0.889029

0.861203

Lingga

0.081887

0.980047

0.766813

0.660457

0.980337

0.982971

0.956784

0.884990

Serapau
Loagan
Bunut
Kayangeran

0.092110

0.020154

0.750547

0.629940

0.968779

0.977598

0.951722

0.896850

0.272747

0.265513

0.286952

0.843882

0.774306

0.757500

0.801043

0.848819

Maludam

0.333878

0.414823

0.462131

0.169743

0.669202

0.659585

0.717615

0.726191

0.076556

0.019859

0.031719

0.255788

0.401670

0.976887

0.970750

0.899795

Manggut

0.101687

0.017175

0.022656

0.277732

0.416145

0.023384

0.968556

0.883434

Betong

0.117625

0.044177

0.049482

0.221840

0.331823

0.029686

0.031949

0.908063

Naman

0.149425

0.122179

0.108867

0.163909

0.319942

0.105589

0.123939

0.096441

Genetic identity and distances differed among populations (Table 7). The genetic identity (Nei
1978) ranged from 0.629940 between Kayangeran and Serapau to 0.982971 between Manggut
and Lingga. The results show that the populations are divided into at least two geographical
regions. Populations from the same geographical region such as Serapau, Maludam, Lingga,
Betong, Sedilu and Manggut have high values of more than 0.9. Similarly, when UPGMA
cluster analysis derived based on Neis gentic distance the populations were clustered into two,
namely the west cluster consisting of Naman, Sedilu, Betong, Maludam, Serapau, Lingga and
Manggut, and the east cluster consisting of Loagan Bunut and Kayangeran (Fig. 3). However it
seems that the west cluster was further separated and positively related to geographical location
when Lingga, Serapau, Manggut, Maludam and Betong populations showed short genetic
distance. The shortest genetic distance was Manggut and Lingga. The five populations were
connected through Lupar Rivers. Similar results were obtained when cluster analysis was
conducted using structure software ver. 2.3 computer program (Fig. 4).
To determine if populations were significantly distinguishable from each other, the pairwise
comparisons of populations was performed. Based on the Fst values, the populations were
significantly differentiated (P< 0.05) from each other except for Manggut, Serapau, Lingga and
Maludam. The Fst values ranged from 0.0042 between Lingga and Manggut to 0.0939 between
Sedilu and Kayangeran (Table 8). The low value of Fst observed between Lingga and Serapau,
Lingga and Maludam, and Lingga and Manggut indicated that they are similar to each other and
is more likely that gene flow would occur along the watercourse.

Figure 3 Dendrogram of genetic distance between populations based on UPGMA generated

using GDA
N a m a n

S e d ilu

15

14

B e to n g

13

M a lu d a m

12

S e ra p a u

17
11

L in g g a
10

M a n g g u t

L o a g a n B u n u t
16

K a y a n g e ra n

___________________________________________________
0.16
0.12
0.08
0.04
0.00
0 .1

Table 8 Genetic differentiation, as estimated by Fst distance among the analysed

populations.

Sedilu

Lingga

Serapau

Loagan
Bunut

Kayangeran

Maludam

Manggut

Betong

Sedilu

0.0000

Lingga

0.0200

0.0000

Serapau
Loagan
Bunut

0.0220

0.0052

0.0000

0.0753

0.0768

0.0811

0.0000

Kayangeran

0.0939

0.1155

0.1234

0.0619

0.0000

Maludam

0.0176

0.0050

0.0081

0.0729

0.1096

0.0000

Manggut

0.0240

0.0042

0.0056

0.0773

0.1113

0.0057

0.0000

Betong

0.0268

0.0116

0.0127

0.0649

0.0951

0.0072

0.0080

0.0000

Naman

0.0332

0.0307

0.0271

0.0502

0.0908

0.0256

0.0301

0.0234

10

Naman

0.0000

Figure 4 Cluster of population based on Structure ver. 2.3. Population divided into two
clusters: Red and Blue. Population 1: Sedilu; 2: Lingga; 3: Serapau; 4: Loagan
Bunut; 5: Kayangeran; 6: Maludam; 7: Manggut; 8: Betong and 9: Naman.

5.0 Conclusions
The effectiveness and efficiency of microsatellite markers to evaluate genetic diversity and
population differentiation demonstrated that microsatellite markers are useful for the
development of DNA database for ramin. The unique alleles detected in specific populations and
signification populations differentiation revealed by cluster analysis and Fst value indicated that
microsatellite markers could be used for population identification. Thus, microsatellite markers
are suitable to generate DNA database for individual timber identification and useful for tracking
of wood in production chain (Tnah et al., 2010; Koopman and Diemont 2004; Smulder et al.,
2008). However, the use of microsatellite markers for identification should not be based on a
limited number of markers but on many loci (Butler 2005). Furthermore, before a marker system
can be introduced into forensic casework, a population database must be established for
statistical evaluation of identity match proof (Tnah, 2010).

6.0 Recommendations
The current ramin DNA database was developed using leaves and bark samples. For actual
timber species identification and tracing and tracking system, samples from wood must be tested.
A new proposal entitled Use of DNA for identification of Gonystylus species and geographical
origin has been submitted to ITTO for funding. The unique alleles detected in specific
populations in Sarawak based on the 18 markers could be detected in other population outside
Sarawak. Thus, the use of more microsatellite markers and sampling across all ramin populations
to other states in Malaysia and neighboring countries is important until population specific
alleles detected. To implement this, support and commitment from respective countries, local
government, policy makers and various NGOs are needed. Once the database is completed, only
then it would be reliable in court proceeding to curb illegal logging enhance the implementation
of CITES legislation/regulations in this region.
11

References
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12

Lim L.S.,WIckneswari R., Lee S.L., Latiff A. 2002. Genetic variation of Dryobalanops aromatic
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13

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14

Long boat is a main transportation used to get to the sampling site.

Peat Swamp Forest at Manggut

15

Peat Swamp Forest at Kayangeran, Lawas

Some remain ramin tree at Naman Forest Reserve

16

Gonystylus bancanus leaves

Staff preparing the PCR mixed for the DNA amplification

17

The PCR amplification was conducted using

GeneAmp PCR System 9700 (Applied Biosystem)

Amplified PCR products are load in ABI 3130x1 Genetic Analyzer

System for fragment analysis

18

Appendix: Genotype Profile of Gonystylus bancanus in Sarawak

Individual
SE001
SE002
SE003
SE004
SE005
SE006
SE007
SE008
SE009
SE010
SE011
SE012
SE013
SE014
SE016
SE017
SE018
SE019
SE020
SE021
SE022
SE023
SE024
SE025
SE026
SE027
SE028
SE029

Gba028
226 246
250 268
222 250
222 250
214 216
214 214
254 258
226 250
214 228
248 278
214 216
214 214
222 250
264 264
222 226
246 248
222 246
222 256
236 270
236 246
236 236
258 264
244 248
214 214
222 248
246 252
236 244
254 264

Gba092
88
96
90 104
90
98
88 104
88
88
86
96
90
90
104 104
88
88
88
98
88
88
88
90
88
96
88
88
90 106
90
90
90
90
96
96
88
88
102 104
88
96
90
96
90 108
88
88
90
90
90 104
90
90
90
90

Gba100
145 163
125 145
145 153
145 155
145 145
144 151
145 162
145 145
145 149
125 125
145 149
143 143
159 159
145 145
145 145
145 145
145 155
155 163
125 125
145 153
125 145
125 145
145 145
149 149
145 153
145 163
145 145
125 125

Gba108
181 186
189 192
188 196
170 186
182 208
182 182
170 170
178 191
182 223
182 186
192 208
208 223
192 209
182 190
190 194
186 192
200 204
195 196
182 198
180 186
189 209
181 190
186 190
223 223
184 195
186 186
170 188
181 189

Gba129
155 157
149 149
149 149
149 149
166 168
166 168
149 163
175 175
168 168
149 149
168 168
164 170
149 175
149 149
149 155
149 155
149 149
149 157
149 171
149 170
149 175
165 171
149 170
164 166
149 149
149 157
169 173
149 169

19

Gba147
142 156
130 148
142 154
144 148
126 130
130 150
136 136
150 150
126 150
136 150
126 126
126 150
130 144
156 156
122 142
144 156
116 136
142 160
130 136
142 165
142 156
130 142
134 148
126 126
142 148
148 160
142 144
144 148

Gba175
189 189
186 186
189 189
189 189
189 189
186 189
186 189
189 189
186 189
186 186
189 189
0
0
186 186
186 189
186 189
186 186
186 189
186 186
186 189
186 189
186 189
186 186
189 189
186 186
186 189
189 189
186 186
186 189

Gba348
106 114
116 122
114 122
126 126
122 126
122 122
114 122
116 116
122 122
114 122
0
0
0
0
122 122
118 126
114 114
116 126
114 126
114 128
106 122
114 114
114 126
114 114
114 116
0
0
114 116
106 128
116 122
114 122

Gba430
132 140
120 120
106 116
116 120
125 125
114 118
114 116
106 114
0
0
110 122
116 124
125 125
116 122
106 114
114 126
106 114
114 118
112 120
116 120
118 140
114 118
116 120
106 118
125 125
106 116
106 112
116 126
120 126

SE030
SE031
SE032
LI001
LI002
LI003
LI004
LI005
LI006
LI007
LI008
LI009
LI010
LI011
LI012
LI013
LI014
LI015
LI016
LI017
LI018
LI019
LI020
LI021
LI022
LI023
LI024
LI025
LI026
LI027

222
254
244
250
260
248
250
226
248
236
250
222
248
258
250
252
256
248
222
248
244
246
248
248
248
236
256
214
226
246

248
290
264
268
266
250
268
228
268
250
254
276
250
264
256
272
264
264
252
248
248
252
252
248
258
246
260
214
250
278

98
90
90
90
88
90
90
94
90
98
90
90
90
90
90
90
88
90
90
96
90
96
90
88
88
90
96
88
94
90

106
104
90
98
90
96
90
120
100
100
92
98
90
100
90
104
90
90
100
104
104
102
106
90
90
104
104
96
98
98

153
145
145
145
125
145
145
145
125
125
145
145
145
145
145
145
145
145
145
145
145
145
145
145
125
145
125
145
145
145

153
145
155
145
145
155
163
145
125
125
145
147
145
145
145
145
145
145
145
145
145
145
145
145
125
149
145
145
145
145

184
190
181
186
186
181
178
186
186
184
188
174
186
176
176
179
178
174
182
189
189
184
182
174
180
186
181
202
170
178

192
190
200
204
192
193
186
188
196
191
194
190
204
186
192
190
188
186
189
190
194
186
189
181
186
188
192
208
186
186

149
149
157
149
149
149
149
156
149
149
157
149
149
149
153
149
149
149
155
155
161
158
157
149
149
149
149
160
149
149

20

157
175
161
149
163
149
149
158
169
169
170
175
169
175
157
171
163
169
161
161
170
165
163
155
175
149
163
166
170
175

122
136
128
126
142
122
130
138
142
146
118
142
142
140
142
124
142
146
118
118
118
142
138
118
122
138
122
126
122
148

142
138
142
140
142
142
142
142
146
154
122
150
146
142
146
142
146
168
146
142
146
146
142
144
154
142
132
161
150
150

186
186
186
186
186
186
189
186
186
186
186
186
186
186
186
189
186
186
186
186
189
186
189
186
186
189
186
186
189
186

189
189
189
189
189
189
189
186
189
189
189
186
186
189
189
189
189
189
186
189
189
186
189
189
189
189
189
186
189
189

114
114
122
126
116
122
116
116
122
114
116
122
122
126
124
114
116
126
122
122
122
122
122
122
116
116
114
122
126
122

114
126
126
126
116
126
130
116
126
126
124
126
130
130
126
116
122
126
126
130
122
122
126
128
130
122
130
130
126
126

114
116
114
124
116
106
106
0
116
114
108
112
112
114
110
112
106
106
116
116
106
116
106
116
106
106
112
125
118
118

118
116
126
126
120
134
122
0
118
118
114
118
120
124
124
122
118
120
116
116
116
120
116
118
112
122
120
125
120
120

LI028
LI029
LI030
SL001
SL002
SL003
SL004
SL005
SL006
SL007
SL008
SL009
SL010
SL011
SL012
SL013
SL014
SL015
SL016
SL017
SL018
SL019
SL020
SL021
SL022
SL023
SL024
SL025
SL026
SL028

248
248
248
252
248
222
248
248
248
248
250
222
250
236
248
238
248
262
250
262
250
248
246
214
248
244
246
252
222
248

248
248
276
264
258
260
276
252
270
262
274
248
250
260
264
250
278
276
270
278
264
264
268
216
272
272
248
268
238
256

88
90
90
88
92
96
90
88
100
90
90
90
90
90
98
98
90
90
90
90
88
90
88
88
96
88
88
88
90
90

98
96
90
90
98
98
100
90
104
90
102
90
100
98
98
104
114
114
90
100
102
96
90
88
100
96
90
90
90
96

145
145
145
145
145
145
145
125
145
145
125
147
145
145
125
145
125
145
145
145
125
145
145
145
125
145
145
145
125
145

145
145
145
159
145
145
155
145
145
145
145
155
145
145
155
145
145
145
155
145
145
153
145
162
153
153
145
145
145
145

186
188
197
186
186
178
170
159
174
174
182
201
170
174
174
193
159
182
180
182
186
186
181
202
180
176
174
186
182
192

188
189
204
192
195
182
174
174
182
182
190
204
192
182
182
194
190
182
182
186
194
186
190
208
192
186
201
194
193
192

149
149
170
149
149
149
155
157
149
149
149
149
149
149
149
149
163
157
149
153
149
163
149
160
149
149
149
149
158
155

21

149
157
175
163
170
149
174
158
174
174
163
149
149
155
149
155
167
169
175
169
175
175
155
166
149
149
170
175
163
163

142
142
142
138
146
142
142
122
118
150
136
126
144
144
118
146
138
124
138
144
118
142
142
126
142
136
118
146
146
126

146
156
142
161
146
142
155
178
124
155
146
138
150
150
142
148
142
150
146
150
142
161
148
154
155
144
156
156
148
150

186
186
186
189
186
186
189
186
189
186
189
186
189
189
186
189
189
186
189
186
189
186
189
186
189
189
186
186
189
186

189
186
189
189
189
189
189
189
189
189
189
189
189
189
190
189
189
189
189
189
189
186
189
186
189
189
186
189
189
186

116
116
122
116
126
116
118
116
114
116
122
114
122
116
118
122
116
124
118
116
116
126
114
106
114
122
116
126
116
122

126
116
126
122
126
126
126
128
126
126
126
126
130
132
126
128
116
126
122
122
124
134
130
106
126
124
122
126
130
130

106
106
114
112
116
118
116
112
112
112
114
112
116
106
116
112
118
110
116
110
118
0
116
0
116
106
106
118
118
116

116
124
118
116
116
118
120
116
116
116
118
116
118
122
120
114
120
116
124
112
120
0
118
0
122
122
118
128
128
124

SL029
SL030
SL031
LB001
LB002
LB003
LB004
LB005
LB006
LB007
LB008
LB009
LB010
LB011
LB012
LB013
LB014
LB015
LB016
LB017
LB018
LB019
LB020
LB021
LB022
LB023
LB024
LB025
LB026
LB027

248
250
248
226
226
256
226
226
256
226
256
270
226
256
248
226
226
256
262
226
248
248
256
256
226
246
226
248
246
246

248
268
272
256
258
270
248
256
276
258
276
276
258
270
256
270
272
266
272
270
272
270
258
270
258
256
258
256
272
268

90
88
90
88
88
88
90
90
88
88
88
90
88
90
90
88
90
88
88
88
90
104
90
88
88
90
90
88
90
90

90
98
90
90
90
104
104
90
90
88
90
104
90
104
90
90
102
90
90
102
90
104
90
102
90
90
90
104
98
90

156
145
145
125
162
145
145
145
145
125
145
145
125
145
145
145
125
145
145
143
125
125
145
145
125
125
143
125
143
125

163
155
145
162
162
145
162
162
145
145
145
155
145
145
145
145
145
145
145
145
145
145
155
155
145
143
145
145
162
143

174
188
174
190
186
188
180
190
180
190
190
188
204
190
186
190
180
188
190
188
188
188
188
188
188
192
188
180
180
180

192
194
186
204
186
203
190
190
182
190
204
190
204
192
188
190
190
190
196
204
188
188
190
195
192
204
204
196
188
204

163
149
149
153
149
153
153
155
155
149
169
155
153
165
153
155
153
155
165
155
153
155
153
155
153
149
155
153
149
149

22

170
170
151
165
153
155
169
169
155
169
171
169
173
169
169
169
165
169
165
169
155
165
173
173
171
149
165
155
153
153

126
118
150
144
146
136
144
142
150
136
150
136
136
142
150
142
142
153
163
136
144
142
142
160
132
144
136
136
144
142

161
146
155
163
163
160
144
144
160
146
153
150
150
144
160
144
160
160
163
142
153
153
144
160
136
160
144
142
155
144

186
189
189
186
189
186
186
186
186
186
189
189
189
186
186
186
186
186
186
186
186
186
186
189
186
186
189
186
186
186

186
189
189
186
189
189
186
189
189
186
189
189
189
186
186
186
189
189
189
186
189
189
189
189
189
189
189
189
189
186

118
114
114
128
128
128
126
126
126
126
128
126
130
126
128
126
126
126
126
128
128
126
128
128
128
126
126
120
126
126

130
116
122
130
128
128
126
130
130
130
130
130
130
126
130
130
130
126
128
128
128
128
130
132
132
128
126
128
128
128

118
136
116
114
114
118
114
114
114
114
116
114
120
106
106
114
114
114
114
114
116
114
114
114
114
106
116
114
106
114

120
136
116
122
114
128
114
120
116
116
128
128
128
114
130
116
114
118
114
116
118
114
128
136
126
114
126
118
118
126

LB028
LB029
LB030
LB031
LB032
LB033
LB034
LB035
LB036
LB037
LB038
LB039
LB040
LB041
LB042
LB043
LB044
LB045
LB046
LB047
LB048
LB049
LB050
LB051
LB052
LB053
LB054
LB055
LB056
LB057

226
226
226
226
248
248
226
226
226
226
258
226
256
226
258
250
246
234
226
256
258
226
226
256
226
226
256
256
258
226

246
226
226
272
270
270
270
246
226
258
270
268
258
226
262
258
250
258
256
268
262
256
256
256
248
280
258
258
272
256

90
88
90
90
90
90
88
88
88
88
90
88
88
88
104
88
88
90
88
90
90
88
88
88
90
90
90
90
88
90

90
90
104
104
90
104
90
88
90
98
90
104
104
90
104
102
90
102
102
104
90
104
104
98
98
90
90
104
90
90

143
143
143
143
143
145
125
145
145
143
145
143
145
145
143
145
145
145
155
145
143
125
143
143
125
145
143
143
143
125

143
145
145
145
155
162
145
145
145
145
145
145
145
145
143
155
145
145
155
145
145
145
145
145
143
155
145
145
145
145

190
204
180
182
180
180
188
180
180
188
186
180
188
188
188
188
204
182
182
188
180
180
188
188
196
170
188
188
188
188

204
204
188
188
180
188
204
184
204
204
188
204
190
188
204
190
204
188
195
188
195
204
188
190
204
188
204
190
188
196

153
149
155
153
155
155
153
155
165
169
155
173
169
153
165
153
155
155
155
170
149
155
153
155
153
155
155
153
165
153

23

165
153
165
153
165
169
155
155
173
173
173
173
173
153
173
155
165
155
169
173
153
155
155
155
155
167
169
165
173
165

142
144
142
142
142
132
144
144
144
144
132
144
144
146
146
155
144
146
144
144
136
144
142
132
132
132
144
142
144
144

144
144
144
160
160
150
146
160
155
153
132
144
155
161
155
163
144
160
160
146
161
146
146
142
142
160
160
144
144
155

186
189
186
186
186
189
186
186
186
186
186
186
186
186
186
186
186
186
186
189
189
186
186
189
189
189
186
186
186
189

189
189
186
186
189
189
186
189
189
189
186
189
189
189
186
186
186
189
186
189
189
189
189
189
189
189
186
186
186
189

126
126
126
128
126
126
126
114
126
126
126
126
126
114
126
126
128
116
126
128
126
126
126
128
128
126
128
126
126
128

126
128
126
130
128
126
128
126
130
126
128
126
126
130
126
128
130
126
126
130
128
126
128
128
128
130
130
130
130
130

114
106
114
116
120
114
114
116
114
114
106
114
114
106
116
106
114
120
118
120
116
116
114
114
114
106
114
114
118
106

126
128
116
116
120
136
118
116
128
128
130
116
114
120
120
116
114
130
120
128
130
124
116
116
116
118
122
118
120
116

LB058
LB059
LB060
LB061
LB062
LB063
LB064
LB065
LB066
LB067
LB068
LB069
LB070
LB071
LB072
LB073
LB074
LB075
LB076
LB077
LB078
LB079
LB080
LB081
LB082
LB083
LB084
LB085
LB086
LB087

270
270
256
246
226
226
256
226
226
226
226
256
256
226
236
236
256
226
0
248
256
226
246
246
226
268
270
248
226
246

270
270
270
256
270
256
258
256
262
226
270
270
256
226
246
276
256
268
0
270
256
256
266
256
256
270
270
256
270
258

88
90
90
88
90
90
88
90
88
88
90
88
88
88
90
90
88
88
90
90
88
90
90
88
88
90
88
90
90
88

90
90
90
90
94
90
88
98
90
98
90
90
90
88
104
90
90
90
96
90
90
90
104
90
104
104
90
104
90
90

125
125
125
143
145
125
125
125
145
125
145
125
125
145
125
125
125
145
125
145
125
143
143
145
143
125
143
145
125
145

143
143
143
145
162
145
145
155
145
145
145
145
145
145
162
143
145
145
143
145
145
145
155
145
155
145
145
155
145
145

190
188
182
182
194
188
180
188
188
188
188
188
180
182
184
182
188
188
0
190
190
188
188
192
190
184
180
188
188
184

204
204
188
190
204
190
188
196
192
194
196
192
188
188
195
184
190
195
0
195
190
190
188
204
195
188
195
188
188
188

153
155
153
155
153
149
173
149
165
149
155
155
153
165
153
153
149
155
0
165
155
149
153
153
155
155
149
153
149
169

24

155
173
173
173
155
157
173
163
165
153
165
169
169
169
173
155
155
165
0
173
173
165
165
169
165
155
169
153
155
169

142
155
132
132
144
142
132
142
144
144
142
132
142
142
136
136
140
142
0
144
132
140
142
161
144
155
132
160
146
136

142
155
144
144
160
144
136
150
148
150
160
160
144
160
142
142
162
155
0
150
142
140
144
161
162
160
148
160
155
144

189
186
186
186
186
186
189
189
186
186
189
186
186
186
186
186
186
186
186
186
189
186
186
186
186
186
186
186
189
186

189
186
186
189
186
186
189
189
186
186
189
189
186
189
186
186
186
186
186
186
189
186
186
186
186
189
189
186
189
186

116
126
126
130
122
126
128
126
126
128
128
128
122
126
126
120
130
128
0
114
128
128
130
128
130
108
122
130
108
126

130
130
130
130
128
130
128
130
126
130
130
130
122
130
128
128
130
128
0
130
130
130
130
130
130
130
128
130
128
128

116
106
118
114
116
114
114
114
116
116
114
106
114
114
120
120
106
116
0
106
120
106
114
114
114
126
130
114
116
116

118
114
118
122
116
124
124
120
116
124
116
118
114
130
122
120
128
122
0
118
120
114
118
118
118
130
130
114
118
116

LB088
LB089
LB090
LB091
LB092
LB093
LB094
LB095
LB096
LB097
LB098
LB099
LB100
LB101
KG001
KG002
KG003
KG004
KG005
KG006
KG007
KG008
KG009
KG010
KG011
KG013
KG014
KG015
KG016
KG017

256
246
226
248
226
266
226
226
226
226
246
246
248
248
246
226
226
246
226
226
246
226
226
226
246
226
248
226
226
226

256
258
274
258
246
268
248
258
266
256
256
258
256
256
256
248
248
276
248
226
276
226
256
226
248
246
268
266
276
270

88
88
90
88
90
88
88
90
88
90
88
88
90
88
88
90
90
90
88
88
88
90
88
88
88
98
90
88
88
88

90
90
90
90
90
90
104
104
98
90
88
88
90
90
90
90
106
98
98
90
106
90
90
88
98
98
98
98
98
90

125
145
125
143
125
143
143
125
125
145
125
125
125
125
125
145
125
125
145
125
155
125
145
145
145
162
155
125
145
145

145
145
145
145
145
145
145
145
145
145
125
125
145
125
162
162
145
162
162
145
162
155
155
155
162
162
162
145
155
155

180
180
188
182
188
184
186
180
190
185
188
180
186
188
180
184
184
188
188
184
186
182
188
184
188
182
188
182
188
188

195
188
188
188
188
190
188
188
203
190
188
188
188
188
184
188
188
192
190
188
188
192
188
184
194
192
188
184
192
192

155
149
165
155
155
163
153
149
165
149
149
153
149
149
155
163
169
155
149
155
169
167
169
155
171
155
155
155
163
155

25

173
173
169
171
173
165
173
155
173
165
155
169
155
173
155
175
173
163
173
163
173
169
171
169
175
169
155
175
163
155

132
154
136
144
142
144
132
144
144
132
162
142
144
136
134
138
144
138
138
138
144
118
136
142
144
132
144
142
144
138

144
160
163
162
160
162
144
160
160
144
162
176
162
144
138
142
146
174
144
138
144
144
177
144
177
138
148
146
144
142

186
186
186
186
186
186
186
186
186
186
186
189
189
186
186
186
186
186
186
186
186
186
186
186
189
186
186
186
186
186

186
186
189
186
189
186
186
186
186
186
186
189
189
186
189
186
189
186
186
186
186
189
189
189
189
189
189
186
186
189

126
130
122
130
114
126
128
124
126
130
128
122
126
128
118
114
126
126
114
128
128
114
116
116
114
128
128
128
114
114

130
130
128
130
126
128
130
128
130
130
130
128
130
130
130
128
128
128
128
128
128
126
128
128
128
128
130
128
128
128

118
114
116
114
114
114
116
106
118
114
118
120
120
114
114
116
106
106
118
114
114
114
106
106
114
114
106
118
118
114

120
124
118
118
122
126
116
114
126
124
128
122
126
116
114
118
118
114
118
118
114
114
118
118
128
114
106
128
122
114

KG018
KG019
KG020
KG021
KG022
KG023
KG024
KG025
KG026
KG027
KG028
KG029
KG030
MA001
MA002
MA003
MA004
MA005
MA006
MA007
MA008
MA009
MA010
MA011
MA012
MA013
MA014
MA015
MA016
MA017

226
248
226
226
226
246
226
248
226
226
248
226
226
258
228
248
268
248
226
222
248
236
260
236
268
248
222
250
248
250

226
276
256
270
246
248
246
268
248
246
248
276
226
268
248
280
276
266
266
246
266
264
280
246
276
268
276
272
264
264

88
98
88
90
88
90
98
88
90
88
88
88
88
90
88
90
98
88
88
90
90
92
88
90
90
90
104
88
90
90

88
98
98
98
98
90
98
98
98
90
90
98
90
90
98
104
104
88
90
90
114
104
90
90
104
90
104
90
94
98

125
125
145
125
125
125
155
145
125
145
145
125
145
145
145
125
145
145
145
145
145
125
145
125
125
145
155
145
145
145

162
145
162
145
145
162
162
145
145
145
155
162
162
145
145
153
145
145
145
145
145
145
153
125
145
145
163
153
167
145

182
188
182
182
184
188
192
184
188
182
188
182
188
194
182
182
182
182
181
182
174
181
174
188
174
170
184
170
184
182

188
188
190
192
192
188
192
188
190
184
188
188
188
194
186
206
196
190
190
197
202
186
196
192
192
184
189
192
186
186

155
155
155
155
157
155
163
155
163
163
167
155
157
149
149
149
149
149
149
149
149
155
153
149
157
153
149
157
149
149

26

173
155
155
167
171
165
165
169
165
169
171
165
171
171
151
175
175
149
155
149
155
167
165
151
157
155
153
170
165
155

136
142
142
138
144
142
134
138
134
142
138
144
142
130
122
126
142
118
142
142
118
140
142
156
142
118
118
142
142
136

173
144
142
160
150
146
134
144
136
160
144
146
146
142
142
142
146
146
142
148
150
142
146
162
142
142
144
142
142
146

186
186
186
186
186
186
186
189
186
189
186
186
186
189
189
186
189
186
186
186
189
189
189
189
186
186
189
186
189
186

186
189
189
186
186
186
186
189
186
189
189
186
186
189
189
186
189
186
186
186
189
189
189
189
186
186
189
186
189
186

126
114
126
114
114
128
126
116
128
126
114
128
128
114
114
114
114
116
116
116
128
122
118
122
122
116
114
122
116
122

126
128
128
128
126
128
128
126
128
130
128
128
128
122
126
122
116
130
128
116
128
124
122
130
130
128
124
122
116
130

114
114
118
118
106
118
106
106
106
118
106
114
106
112
108
112
106
120
106
120
106
116
106
116
106
106
112
114
112
116

118
118
128
118
122
122
118
106
118
130
122
114
106
118
120
118
112
124
106
128
106
120
112
120
116
124
118
116
116
124

MA018
MA019
MA020
MA021
MA022
MA023
MA024
MA025
MA026
MA027
MA028
MA029
MA030
MA031
MA032
MA033
MA034
MA035
MA036
MN001
MN002
MN003
MN004
MN005
MN006
MN007
MN008
MN009
MN010
MN011

226
248
222
226
248
248
262
248
250
252
252
264
248
248
250
222
226
248
250
264
246
222
250
244
252
248
222
248
248
248

238
248
248
260
250
248
264
268
290
262
264
280
264
274
264
248
264
258
264
272
248
266
262
262
268
274
248
256
276
250

88
90
88
88
88
90
90
90
90
88
88
88
88
88
88
90
88
90
90
88
88
90
88
96
90
88
96
88
90
90

90
90
90
90
90
98
102
112
98
90
90
98
94
90
104
90
114
90
98
90
96
90
104
108
98
90
98
96
90
90

125
145
145
125
134
145
145
145
145
134
155
125
145
143
145
125
145
153
125
143
143
125
145
145
155
145
145
145
145
145

145
153
153
145
145
145
167
155
145
155
155
145
147
145
147
159
145
153
125
143
143
145
155
155
155
145
147
155
155
157

188
174
174
186
174
170
182
184
186
180
186
181
182
182
186
182
180
170
174
181
186
192
181
181
182
182
184
184
170
184

191
182
186
191
188
181
186
206
186
184
186
181
193
186
186
193
198
186
192
188
190
198
181
204
184
188
194
190
182
188

149
155
149
149
163
149
157
153
149
155
155
149
149
149
165
163
149
149
149
149
149
149
149
149
149
153
155
149
149
149

27

149
163
163
163
169
155
165
163
153
163
157
153
153
157
175
175
165
165
155
155
163
149
153
149
169
155
167
170
155
157

138
132
142
144
142
150
112
122
130
118
142
118
142
146
124
138
124
0
142
138
136
124
146
134
126
130
142
126
126
136

142
150
142
165
144
150
161
146
150
136
150
118
157
150
146
142
130
0
142
150
150
165
153
165
140
140
160
142
142
146

186
186
186
189
186
186
189
186
189
186
189
186
189
186
189
189
186
189
186
186
189
189
186
189
186
189
186
189
186
186

186
186
189
189
189
189
189
186
189
189
189
189
189
189
189
189
189
189
186
186
189
189
189
189
186
189
186
189
189
186

122
124
114
114
122
118
116
114
116
122
122
116
126
116
116
126
116
114
130
118
114
114
114
122
122
116
124
122
102
114

126
130
116
126
124
130
126
116
118
128
128
122
128
122
126
126
126
114
134
130
122
122
122
122
128
130
126
126
126
122

108
118
116
108
120
116
112
106
112
120
116
116
114
116
116
112
118
114
116
116
114
106
106
118
112
106
112
120
106
106

122
120
122
120
122
120
112
126
112
124
124
122
116
120
120
120
124
122
122
120
120
112
120
124
124
122
112
120
118
122

MN012
MN013
MN014
MN015
MN016
MN017
MN018
MN019
MN021
MN022
MN023
MN025
MN026
MN027
MN028
MN029
MN030
MN031
MN032
MN033
MN034
MN035
MN036
MN037
MN038
MN039
MN040
MN041
MN042
MN043

250
222
248
248
244
250
236
248
248
246
250
248
272
262
250
248
226
254
242
268
250
222
250
248
236
248
250
250
250
226

252
248
250
262
250
260
250
272
248
250
250
262
276
266
266
262
248
264
248
274
252
264
264
248
276
262
264
268
256
276

96
90
90
88
92
96
96
90
88
90
90
90
90
88
88
90
88
90
104
88
90
88
90
90
98
90
90
88
90
88

98
96
96
90
98
96
98
90
104
112
90
106
98
106
98
106
96
96
114
88
104
90
98
112
98
90
96
88
90
90

145
145
145
147
145
155
125
145
145
145
145
145
125
145
145
147
125
125
143
145
145
125
153
145
145
145
145
125
145
145

145
153
145
147
145
167
145
145
145
147
163
145
145
145
145
159
125
145
143
145
145
145
155
145
145
145
145
145
163
153

159
195
182
161
174
182
182
180
190
181
170
182
182
186
186
186
190
181
182
174
174
181
180
170
184
174
182
188
186
159

195
201
204
174
181
186
184
182
194
195
195
186
182
186
190
186
194
195
186
186
182
186
184
182
184
184
190
192
196
189

149
158
149
149
155
149
149
153
149
149
163
149
149
153
153
149
157
149
155
149
155
149
163
149
149
149
149
155
163
149

28

153
173
151
149
163
170
163
155
155
149
163
161
171
155
170
155
171
149
155
149
161
149
169
170
155
155
149
169
175
155

142
131
146
148
146
118
142
138
146
136
138
138
134
0
126
118
142
140
126
146
134
142
130
130
118
146
146
138
142
154

142
142
156
157
155
146
173
138
146
140
140
150
146
0
142
138
146
150
140
165
142
156
146
146
173
173
146
163
150
154

186
189
189
186
186
186
189
189
189
186
186
189
189
186
186
189
189
189
186
189
189
186
186
189
189
186
186
186
186
189

186
189
189
186
189
186
189
189
189
186
186
189
189
186
186
189
189
189
189
189
189
186
189
189
189
189
186
186
189
189

116
124
116
114
122
116
116
114
116
114
116
126
116
116
116
116
130
114
116
116
116
130
114
122
116
108
126
108
122
114

130
128
124
122
126
130
122
130
130
130
128
126
130
122
122
126
130
122
116
122
122
130
122
122
130
122
137
116
130
137

120
112
112
106
112
116
106
118
124
116
116
112
118
120
116
116
118
108
108
112
106
112
118
120
120
106
120
116
118
116

120
122
120
112
124
120
120
120
134
120
122
130
124
134
124
134
134
120
114
116
116
118
120
130
120
116
124
118
118
120

MN044
MN045
MN046
MN047
MN048
MN049
MN050
MN051
MN052
MN053
MN054
MN055
BE001
BE002
BE003
BE004
BE005
BE006
BE007
BE008
BE009
BE010
BE011
BE012
BE013
BE014
BE015
BE016
BE017
BE018

236
250
248
258
230
262
250
256
250
248
246
262
250
248
248
228
248
248
248
248
244
256
250
248
250
248
222
254
226
248

248
268
258
280
268
264
250
264
272
248
266
264
264
278
264
248
248
250
250
264
246
258
290
290
250
270
266
276
246
258

88
88
96
90
90
90
90
88
98
88
90
90
88
90
90
90
90
90
88
90
90
98
88
88
90
90
96
88
90
90

90
96
98
96
90
90
90
96
102
108
90
106
98
104
90
90
96
104
90
104
98
104
98
90
98
90
98
90
96
90

145
145
145
125
125
145
145
145
145
145
145
145
145
145
145
125
125
153
145
155
143
145
145
125
125
145
145
125
125
125

153
145
145
145
145
145
145
167
145
145
145
147
145
155
155
153
125
153
145
155
155
163
155
145
145
145
145
125
167
125

174
174
182
174
204
178
182
182
174
166
186
186
184
186
186
190
194
159
159
186
186
184
182
188
181
170
181
188
174
174

194
182
201
201
204
186
182
194
182
201
195
186
186
190
197
196
196
186
194
188
206
186
190
190
184
184
188
204
188
180

149
149
155
151
155
149
149
149
155
153
153
149
155
153
149
155
153
149
149
149
163
149
149
153
153
149
149
149
149
149

29

155
163
162
163
163
149
149
153
171
153
167
155
167
153
170
171
163
155
155
169
163
163
165
169
172
155
163
171
167
167

142
120
136
136
146
142
142
142
118
144
138
118
130
138
150
134
146
138
148
138
138
136
126
126
138
142
150
126
136
146

146
146
146
150
150
146
142
146
146
173
140
138
138
140
150
168
150
146
157
144
146
144
150
126
150
146
165
138
142
146

189
189
186
186
186
189
189
189
189
186
186
186
186
189
186
186
189
189
189
186
186
186
189
189
186
186
189
189
186
186

189
189
189
186
186
189
189
189
189
189
186
189
189
189
189
189
189
189
189
186
186
186
189
189
189
186
189
189
189
186

114
114
122
124
114
122
126
122
114
128
124
116
126
122
116
122
126
114
116
116
116
112
126
116
132
116
114
126
116
116

114
130
122
130
122
122
130
130
126
128
126
126
130
126
130
130
130
126
116
126
126
130
139
139
132
126
132
134
130
126

114
116
112
106
106
116
118
118
106
118
120
106
116
114
114
106
106
106
116
112
120
116
106
106
116
106
118
112
124
124

120
124
122
120
122
116
120
120
116
120
122
134
124
116
120
116
116
118
116
116
122
126
116
116
116
126
124
124
124
124

BE019
BE020
BE021
BE022
BE023
BE024
BE025
BE026
BE027
BE028
BE029
BE030
BE031
BE032
BE033
BE034
BE035
BE036
BE037
BE038
BE039
BE040
NA001
NA002
NA003
NA004
NA005
NA006
NA007
NA008

248
226
250
250
248
262
248
248
248
248
222
248
248
244
252
248
250
248
226
238
238
248
262
270
246
250
248
248
246
248

272
274
252
258
286
268
252
264
264
250
268
252
258
252
276
276
268
254
250
286
238
250
268
274
262
260
250
256
246
256

90
90
88
90
90
90
88
90
90
90
96
98
90
96
90
90
88
90
90
90
90
90
90
90
90
88
90
90
90
104

114
90
90
98
90
90
88
98
90
98
98
98
98
98
90
100
90
98
98
98
98
96
90
104
104
90
90
104
90
104

125
145
163
145
125
145
145
145
143
145
145
145
145
125
145
125
145
145
145
125
125
125
149
125
145
145
125
145
125
145

145
145
163
155
145
145
151
161
145
155
145
145
155
145
145
145
145
145
147
145
145
155
149
145
145
145
157
157
145
155

186
186
186
181
170
180
174
174
189
188
174
174
174
180
197
188
166
170
174
170
174
193
190
190
180
194
190
188
180
159

194
192
200
191
186
201
181
180
196
192
186
182
174
186
201
192
182
186
194
174
187
198
192
204
188
194
194
190
186
197

149
159
149
163
153
155
153
145
149
153
155
149
158
155
149
155
155
149
155
149
153
155
149
153
149
149
149
149
149
155

30

149
163
169
163
175
155
155
155
167
157
155
162
175
163
149
167
163
155
163
153
170
155
170
171
157
149
155
169
163
175

116
136
140
142
136
116
142
140
138
146
150
146
140
118
150
146
142
146
142
146
138
124
0
142
122
142
140
142
142
138

138
146
142
146
146
161
150
144
150
150
161
156
144
140
156
150
146
160
146
146
140
126
0
160
146
160
156
156
142
150

189
186
186
186
186
189
189
189
186
189
186
186
189
186
186
189
189
186
189
186
186
186
186
186
186
186
186
186
186
189

189
189
186
186
189
189
189
189
189
189
186
189
189
189
189
189
189
186
189
186
186
189
189
186
190
189
186
186
186
189

122
122
114
116
114
116
116
114
116
116
116
114
114
122
122
130
116
114
114
114
130
122
126
114
126
126
130
122
126
106

130
122
114
130
124
124
118
124
122
122
122
122
134
126
130
130
126
122
130
130
130
139
130
116
126
130
130
130
128
116

116
106
116
108
118
116
106
116
106
118
114
114
114
116
106
116
112
106
104
116
118
112
106
106
116
106
122
122
106
116

116
112
124
112
118
122
112
118
112
118
116
116
114
118
116
140
118
118
112
116
118
124
130
106
120
122
122
134
116
124

NA009
NA010
NA011
NA012
NA013
NA014
NA015
NA016
NA017
NA018
NA019
NA020
NA021
NA022
NA023
NA024
NA025
NA026
NA027
NA028
NA029
NA030
NA031
NA032
NA033
NA034
NA035
NA036
NA037
NA038

266
216
236
236
276
248
252
262
268
256
252
246
246
258
262
250
224
250
236
228
246
250
248
248
248
236
246
254
268
250

272
216
254
236
276
254
270
262
270
272
272
250
272
274
276
274
256
264
264
228
248
250
276
248
256
252
248
272
272
256

90
88
90
90
90
90
90
90
90
90
88
88
98
98
90
90
90
90
90
88
88
90
90
88
90
90
90
104
90
90

90
90
104
90
100
90
90
106
98
96
90
104
98
104
104
90
90
104
90
88
92
90
90
104
90
90
90
104
102
102

125
125
125
145
125
125
145
145
147
145
143
145
0
145
125
145
145
145
125
125
125
125
125
125
145
145
145
145
145
125

155
125
145
145
155
125
161
161
161
147
143
159
0
155
125
147
151
155
125
145
145
149
145
145
153
149
153
145
155
145

192
192
192
190
195
194
184
180
187
178
186
182
188
178
187
180
180
186
186
184
178
190
190
180
180
188
187
182
184
188

206
200
200
204
201
204
195
182
195
186
194
190
188
204
194
187
190
188
194
184
188
194
192
184
190
188
190
188
196
188

153
160
160
155
149
149
155
149
149
153
149
149
157
149
170
149
155
149
157
149
157
155
149
149
155
163
163
155
155
149

31

155
164
164
157
157
157
157
157
170
170
175
149
157
149
175
158
158
157
175
155
165
175
157
167
167
169
163
163
169
175

136
126
138
146
122
142
142
142
142
144
138
142
142
142
118
144
142
134
160
136
142
144
142
140
140
122
138
142
140
144

142
130
150
150
142
150
154
144
154
146
146
144
142
150
144
146
144
142
163
160
155
156
144
142
142
134
148
144
156
154

189
189
189
189
189
189
189
189
189
186
186
186
0
186
189
189
186
186
186
186
189
186
189
186
186
189
186
186
186
186

189
189
189
189
189
189
189
189
189
189
189
189
0
186
189
189
186
189
189
189
189
186
189
189
189
189
189
189
189
189

116
0
0
126
116
126
126
114
126
126
114
124
128
106
126
126
126
114
122
114
114
126
114
114
126
122
126
126
106
116

130
0
0
130
130
130
126
126
130
126
126
126
128
116
126
128
126
122
122
126
122
126
116
122
130
130
126
130
126
126

116
125
114
122
116
120
114
114
114
122
124
114
0
118
118
118
114
124
116
114
106
112
118
116
116
116
114
122
126
108

122
125
140
140
140
140
116
114
124
134
140
122
0
122
118
134
128
142
120
116
106
120
120
134
118
120
116
126
142
116

NA039
NA040
NA041
NA042
NA043
NA044
NA045
NA046
NA047
NA048
NA049
NA050
NA051
NA052
NA053
NA054
NA055

246
262
248
272
226
246
256
246
246
246
238
246
238
248
228
256
230

274
268
248
276
274
262
264
276
266
250
266
260
248
276
274
272
280

92
90
90
90
90
90
90
88
90
90
88
88
90
90
90
88
88

92
104
90
90
106
90
102
90
104
104
90
98
90
90
104
88
90

125
125
145
125
145
153
145
125
145
149
125
125
125
147
125
145
125

155
145
145
145
153
164
145
145
145
159
145
143
145
151
145
145
164

186
188
194
192
182
170
176
190
188
159
180
182
170
194
188
184
184

190
196
198
200
187
178
190
192
192
190
186
188
184
194
192
204
204

153
161
149
149
153
155
0
149
149
153
155
157
170
155
151
149
153

32

175
163
157
170
167
171
0
163
155
163
173
170
170
163
170
153
175

142
140
136
134
144
136
124
142
136
144
136
142
144
142
132
142
142

142
142
156
136
144
142
150
142
142
156
142
150
148
144
144
144
158

189
186
189
189
186
189
186
186
189
189
189
189
186
186
189
189
186

189
189
189
189
189
189
186
189
189
189
189
189
189
189
189
189
189

126
114
114
120
122
126
116
114
126
114
120
114
114
122
126
122
116

130
126
126
130
126
126
130
126
126
128
122
122
122
122
130
126
126

110
106
106
114
122
106
108
114
120
114
118
118
118
122
120
108
118

114
136
124
126
122
106
116
120
120
114
120
118
122
122
134
112
122

Ind.
SE001
SE002
SE003
SE004
SE005
SE006
SE007
SE008
SE009
SE010
SE011
SE012
SE013
SE014
SE016
SE017
SE018
SE019
SE020
SE021
SE022
SE023
SE024
SE025
SE026
SE027
SE028
SE029
SE030

WGb06
196 199
196 199
199 199
187 199
196 196
196 196
187 196
199 199
195 195
187 199
183 198
195 198
199 199
187 199
187 196
199 199
187 196
192 196
190 199
196 199
199 199
199 199
177 199
196 196
199 199
199 199
187 196
196 199
187 199

WGb17
321 333
321 342
318 352
339 342
324 330
324 324
324 358
330 336
321 321
318 339
321 321
321 321
321 339
318 321
318 321
324 339
318 349
318 336
349 370
318 342
318 321
324 330
339 349
324 324
321 352
349 352
330 336
327 330
318 327

WGb22
282 284
276 288
272 284
270 284
272 272
0
0
242 284
270 294
272 272
284 296
0
0
0
0
284 284
284 284
270 284
272 286
242 286
284 284
272 284
282 282
270 282
270 296
270 270
272 272
270 284
272 286
284 292
272 280
270 286

WGb24
332 345
345 349
332 349
345 347
332 332
332 332
332 345
332 332
332 336
332 347
332 332
332 332
323 332
345 351
345 351
332 332
332 349
332 349
332 332
349 349
323 351
332 334
328 345
332 332
332 332
323 345
332 347
332 332
332 351

WGb29
135 146
135 135
118 122
111 137
102 102
104 104
135 135
135 141
102 102
122 135
102 104
104 104
122 122
128 143
135 135
135 141
104 135
122 135
135 135
104 135
143 143
122 135
126 135
104 104
111 135
111 135
135 135
128 135
111 128

33

WGb32
295 317
303 307
305 315
309 331
293 303
285 293
303 309
309 311
293 293
293 297
293 303
285 293
297 309
305 325
309 333
278 323
297 309
309 319
299 305
297 323
299 305
303 305
278 315
293 303
291 291
291 303
303 307
303 323
305 309

WGb37
169 173
169 171
162 169
171 171
169 175
169 169
173 173
171 173
169 169
162 171
169 169
169 169
169 171
171 173
173 173
162 169
162 173
173 173
169 173
162 169
162 171
169 173
162 169
169 169
162 162
162 173
173 173
169 173
169 173

WGb38
275 275
275 275
281 283
281 283
269 269
269 269
281 283
275 283
269 269
279 283
259 269
269 269
279 281
281 281
281 283
281 283
281 283
275 283
279 281
281 283
283 283
281 283
281 287
269 269
283 283
275 283
283 283
281 283
281 283

WGb39
164 164
154 164
154 160
164 168
0
0
0
0
160 160
160 162
0
0
162 166
0
0
0
0
162 162
160 162
160 164
160 162
160 160
164 164
160 164
160 160
160 162
162 166
160 162
156 168
160 162
162 166
160 164
160 160
162 162

SE031
SE032
LI001
LI002
LI003
LI004
LI005
LI006
LI007
LI008
LI009
LI010
LI011
LI012
LI013
LI014
LI015
LI016
LI017
LI018
LI019
LI020
LI021
LI022
LI023
LI024
LI025
LI026
LI027
LI028

199
187
199
177
177
199
197
199
199
199
199
199
196
196
199
199
199
177
196
199
199
199
199
199
199
199
195
187
187
199

199
199
199
199
199
199
197
199
199
199
199
199
196
199
199
199
199
199
199
199
199
199
199
199
199
199
195
199
199
199

321
330
330
327
336
327
324
321
327
321
321
339
321
321
321
321
330
321
321
330
318
324
327
321
324
318
324
321
318
318

333
339
330
333
367
339
376
327
330
346
376
373
321
339
342
339
339
324
330
349
327
342
336
321
330
349
324
324
318
330

284
270
270
272
284
284
280
270
284
272
282
270
270
272
284
296
270
284
270
270
272
284
272
284
282
280
284
270
272
270

284
282
284
284
286
292
284
294
294
284
292
270
284
284
284
296
286
284
290
270
284
294
284
290
284
284
284
284
274
270

347
332
332
332
332
323
325
332
332
323
323
349
332
334
332
332
347
323
332
345
323
323
332
332
332
323
336
334
332
332

349
332
345
332
351
332
331
332
349
349
323
349
334
351
349
347
349
323
345
345
332
332
351
334
347
345
336
334
334
332

126
135
126
120
122
111
111
122
135
122
135
122
135
135
122
139
143
122
122
122
139
126
126
126
148
122
102
133
122
122

34

126
135
128
126
122
146
120
135
148
126
148
126
148
135
148
146
148
124
135
148
139
133
126
148
160
126
104
135
139
135

291
293
303
303
291
303
293
295
311
303
305
317
305
295
307
291
291
293
299
305
307
299
297
297
289
295
293
293
309
305

309
315
313
319
297
303
293
309
315
305
305
329
311
305
321
315
313
315
299
315
315
317
313
303
297
315
293
309
321
313

162
162
162
169
162
162
0
162
162
169
169
162
169
162
162
162
169
171
169
169
173
162
162
162
169
171
169
173
162
162

169
177
171
173
173
173
0
162
171
171
173
162
171
169
171
169
173
171
173
177
181
171
169
171
173
173
171
173
173
177

275
275
275
281
275
275
283
281
281
275
287
281
281
275
275
275
281
281
281
281
275
281
275
281
283
275
269
281
281
285

283
287
285
281
275
283
283
287
281
287
287
281
283
275
281
283
283
285
285
281
281
283
283
283
287
281
269
287
281
287

162
164
160
154
160
160
161
160
166
162
160
160
160
160
162
154
162
160
160
160
160
160
162
160
160
160
0
160
160
162

171
166
164
160
162
168
173
166
168
162
160
164
160
160
162
166
168
160
162
160
162
162
162
168
168
164
0
162
162
168

LI029
LI030
SL001
SL002
SL003
SL004
SL005
SL006
SL007
SL008
SL009
SL010
SL011
SL012
SL013
SL014
SL015
SL016
SL017
SL018
SL019
SL020
SL021
SL022
SL023
SL024
SL025
SL026
SL028
SL029

177
196
177
199
199
196
196
199
196
199
199
199
199
199
199
199
199
199
199
196
196
187
195
177
196
199
199
199
196
199

196
199
199
199
199
199
199
211
199
199
199
199
199
211
199
199
199
199
199
199
199
199
198
199
196
199
199
199
199
199

330
327
330
321
318
321
318
321
321
321
321
318
327
321
321
318
318
321
318
321
321
321
324
330
330
321
327
330
339
355

333
330
346
321
321
321
339
336
330
330
370
321
330
330
330
327
349
321
321
339
321
339
324
339
361
330
330
361
364
364

272
272
272
286
282
272
270
284
284
270
272
270
284
270
270
270
270
270
270
284
270
290
0
284
270
286
270
270
270
284

294
272
296
286
282
284
282
284
284
296
292
280
292
284
284
284
274
290
296
292
270
292
0
284
282
286
292
272
284
294

323
332
325
334
332
325
345
349
332
334
349
323
332
334
323
332
332
332
325
332
332
323
332
332
345
323
332
332
332
332

332
336
349
345
349
349
345
349
332
349
351
349
332
351
349
345
345
332
332
351
351
334
332
332
349
334
334
332
332
332

135
122
135
122
122
122
122
124
124
122
124
124
111
135
124
118
126
126
122
130
122
126
102
133
124
118
124
126
126
124

35

148
124
139
148
148
124
124
124
139
124
126
126
122
146
148
126
126
148
126
148
122
148
102
148
135
122
141
133
135
139

305
295
297
297
299
293
305
307
303
297
297
295
299
297
295
291
289
307
293
293
293
305
293
293
303
297
299
291
297
295

305
317
305
307
299
307
311
307
307
307
309
307
315
307
323
323
297
323
305
323
297
325
293
293
305
303
303
299
305
297

171
173
171
162
171
169
169
169
169
162
162
162
162
162
162
162
162
169
173
169
169
173
169
162
162
162
162
169
171
173

173
173
173
173
173
171
173
177
173
173
173
173
169
173
179
173
169
179
173
173
171
173
169
173
171
171
171
179
173
173

281
283
283
281
275
275
281
275
281
275
275
275
281
275
281
281
281
275
281
283
275
281
269
275
285
283
283
283
275
283

287
285
283
281
287
283
287
281
283
275
285
285
287
283
287
281
289
287
283
283
275
287
269
283
287
287
287
283
285
283

160
160
160
160
162
154
162
164
154
160
160
164
160
160
160
160
162
160
160
160
160
160
0
162
160
160
160
160
160
160

166
162
160
164
164
162
164
168
164
168
160
164
164
164
160
164
162
166
162
160
164
160
0
168
160
162
164
164
160
160

SL030
SL031
LB001
LB002
LB003
LB004
LB005
LB006
LB007
LB008
LB009
LB010
LB011
LB012
LB013
LB014
LB015
LB016
LB017
LB018
LB019
LB020
LB021
LB022
LB023
LB024
LB025
LB026
LB027
LB028

199
199
196
177
196
187
187
177
199
199
196
199
196
199
196
196
196
196
199
199
199
187
187
199
197
197
199
196
199
196

199
199
199
199
199
199
199
199
199
199
196
199
199
199
196
199
199
199
199
199
199
199
199
199
199
199
199
197
199
197

318
321
324
324
321
333
318
321
318
318
324
324
318
321
318
324
321
330
330
318
318
318
318
318
318
318
321
318
318
318

318
367
330
324
324
352
324
324
321
318
324
333
324
339
318
330
333
333
358
324
324
318
330
333
324
318
324
318
342
330

282
270
270
270
270
270
270
270
270
270
270
270
270
270
270
270
270
270
270
270
270
270
270
270
270
270
270
270
286
270

284
292
282
270
270
270
270
284
270
286
270
270
286
270
270
270
270
282
270
272
270
270
272
272
272
270
288
286
286
270

332
323
323
332
332
353
345
334
334
323
323
323
332
323
323
341
341
323
332
332
345
347
341
332
332
332
345
341
341
332

349
334
353
353
345
353
353
334
351
338
353
347
341
347
341
353
353
345
341
341
351
353
353
353
341
353
353
353
353
353

122
135
143
135
137
133
143
137
135
135
135
133
135
135
135
122
133
143
135
135
135
135
135
133
133
135
135
135
135
135

36

128
148
143
143
141
143
143
143
143
135
135
135
143
135
143
143
133
143
143
143
143
143
143
135
135
143
141
137
135
143

303
293
295
305
305
289
307
295
295
305
319
295
305
293
289
299
305
295
295
295
295
299
309
299
303
305
297
299
319
299

305
301
295
319
319
305
313
305
295
319
319
319
313
319
295
319
311
305
317
331
295
313
313
319
331
311
305
331
331
311

169
169
169
169
169
173
162
169
162
169
162
169
162
169
162
173
169
162
169
162
162
173
162
162
162
171
169
169
169
162

177
171
173
169
171
177
169
173
169
173
173
173
173
173
173
173
173
173
173
171
173
173
173
173
171
173
169
171
171
162

283
275
281
283
281
275
281
275
275
283
275
283
281
281
281
281
281
275
283
281
283
281
281
281
281
281
275
275
281
281

285
283
283
283
287
275
281
281
283
287
281
287
281
283
283
281
283
281
283
283
283
283
281
283
281
281
275
281
281
281

160
160
164
164
162
164
160
164
160
164
164
160
160
160
162
160
160
160
162
160
164
164
160
160
160
160
162
160
160
162

162
164
166
166
164
164
162
166
164
164
164
164
160
164
164
160
164
160
164
160
164
164
164
164
160
164
164
160
162
166

LB029
LB030
LB031
LB032
LB033
LB034
LB035
LB036
LB037
LB038
LB039
LB040
LB041
LB042
LB043
LB044
LB045
LB046
LB047
LB048
LB049
LB050
LB051
LB052
LB053
LB054
LB055
LB056
LB057
LB058

196
199
196
199
196
199
196
196
199
177
199
199
199
199
196
199
196
196
196
196
199
199
199
196
196
187
196
199
196
199

197
199
196
199
199
199
199
199
199
199
199
199
199
199
199
199
196
199
196
199
199
199
199
199
199
199
199
199
199
199

318
321
318
324
318
315
321
318
318
318
321
324
318
324
318
318
318
318
318
318
321
324
333
324
324
321
321
318
318
318

361
352
336
339
355
318
355
321
318
321
321
336
321
324
336
324
318
352
324
318
324
330
336
333
324
324
324
321
361
318

270
270
270
270
270
270
270
270
270
270
270
270
270
282
270
270
270
284
270
270
272
270
270
270
270
270
270
270
270
270

286
270
270
284
270
272
272
270
270
270
270
270
272
284
270
284
270
288
284
270
284
272
270
270
270
270
288
272
270
270

341
332
334
341
332
323
341
341
323
323
323
323
351
332
341
347
323
353
341
323
345
332
323
347
332
332
341
341
323
341

345
353
334
347
345
332
345
345
347
334
323
353
353
353
351
353
353
353
353
345
353
347
323
353
353
353
353
347
347
353

133
143
128
141
135
133
135
135
133
141
133
133
137
143
133
133
137
141
141
133
133
133
135
135
137
137
133
133
133
133

37

135
143
137
143
137
143
143
143
143
141
143
139
143
143
135
141
141
143
143
135
143
143
143
143
137
137
143
141
135
137

319
295
295
295
295
301
305
305
295
295
295
295
305
295
293
293
295
289
295
295
295
295
295
295
319
293
305
301
301
295

331
295
319
319
299
311
311
311
295
295
297
309
313
303
313
305
295
299
313
317
311
295
295
319
321
305
311
311
307
305

169
162
169
162
169
171
169
169
169
162
169
162
169
169
169
169
169
169
169
173
169
169
173
162
169
169
162
169
162
162

171
173
173
169
173
173
173
173
173
171
173
169
173
173
173
173
169
173
173
173
173
173
173
169
173
173
173
171
173
173

281
275
281
283
281
281
281
281
281
281
275
275
275
275
283
283
281
281
275
281
281
275
281
281
281
281
281
281
281
275

281
281
283
287
283
281
281
283
283
287
281
275
281
283
283
283
287
283
281
283
281
283
283
281
281
287
283
287
283
281

160
160
160
160
160
160
160
160
164
160
164
164
162
164
164
164
164
164
160
160
164
160
164
162
160
160
160
162
160
160

160
164
160
164
164
164
164
164
164
162
166
166
164
164
164
164
164
164
164
160
164
160
164
162
160
166
160
166
164
162

LB059
LB060
LB061
LB062
LB063
LB064
LB065
LB066
LB067
LB068
LB069
LB070
LB071
LB072
LB073
LB074
LB075
LB076
LB077
LB078
LB079
LB080
LB081
LB082
LB083
LB084
LB085
LB086
LB087
LB088

187
199
196
187
196
197
196
199
199
199
197
177
197
199
196
199
196
199
199
177
199
199
196
199
196
199
199
199
199
196

196
199
199
199
199
199
199
199
199
199
199
199
199
199
199
199
199
199
199
199
199
199
199
199
199
199
199
199
199
199

318
318
324
318
324
318
318
318
318
318
318
318
324
318
318
318
318
0
318
318
318
318
318
318
318
318
318
327
318
318

333
324
330
352
333
324
330
333
330
321
318
349
333
318
318
333
324
0
318
367
333
352
324
321
318
324
324
349
318
324

270
270
270
272
270
272
270
270
270
270
270
270
270
270
270
270
270
284
270
270
270
270
270
270
270
270
282
270
270
270

272
272
272
272
270
288
270
288
284
270
284
272
272
270
270
270
272
286
272
288
270
284
270
270
270
272
284
284
272
270

323
332
334
323
347
341
323
351
351
323
332
0
341
347
347
332
334
0
347
323
341
323
334
323
332
347
341
332
347
332

349
334
353
334
351
347
353
353
351
341
353
0
341
351
347
341
351
0
351
353
353
353
347
341
349
351
347
347
347
332

139
126
126
133
133
133
133
141
133
126
133
135
122
135
135
135
135
133
128
135
143
135
126
135
139
143
135
135
135
133

38

143
141
143
143
133
137
135
143
135
143
135
135
135
137
135
139
135
139
141
143
143
135
133
135
143
143
143
143
141
143

295
295
289
303
303
295
305
295
299
305
305
299
313
295
295
295
305
0
305
295
295
295
293
295
299
305
293
299
299
289

319
303
295
319
305
331
305
311
305
321
319
313
317
319
313
313
309
0
309
311
295
299
295
299
305
311
295
319
311
295

162
162
162
169
173
162
169
173
173
162
162
171
169
169
173
169
169
0
169
162
162
171
169
169
162
162
169
162
162
169

169
171
169
173
173
162
173
173
173
173
169
173
173
171
173
173
173
0
173
173
173
173
169
173
171
169
173
173
173
169

275
281
287
275
281
281
281
281
281
275
283
281
283
281
281
281
275
0
281
275
281
281
275
275
275
283
281
283
275
281

275
281
289
283
281
283
287
283
281
281
283
281
283
283
281
287
281
0
283
281
281
283
281
281
283
283
283
283
283
281

160
162
160
164
162
162
160
162
160
160
162
162
160
160
164
160
164
160
160
160
160
162
160
164
160
162
164
162
162
162

164
162
164
164
164
164
160
164
164
164
164
162
164
162
164
160
164
162
160
164
164
164
160
164
160
164
164
162
164
164

LB089
LB090
LB091
LB092
LB093
LB094
LB095
LB096
LB097
LB098
LB099
LB100
LB101
KG001
KG002
KG003
KG004
KG005
KG006
KG007
KG008
KG009
KG010
KG011
KG013
KG014
KG015
KG016
KG017
KG018

199
199
197
199
196
199
199
199
196
199
196
199
199
196
196
177
196
199
196
177
196
199
196
177
196
177
196
196
199
177

199
199
199
199
199
199
199
199
199
199
199
199
199
196
199
196
196
199
196
199
199
199
199
196
196
199
196
196
199
196

321
318
318
318
318
318
318
318
324
321
318
318
321
324
324
318
324
324
318
318
318
318
324
324
324
318
343
346
324
318

324
333
352
330
318
318
324
333
333
336
324
324
324
324
346
346
324
324
318
324
318
349
352
346
333
333
343
352
346
346

270
270
270
270
270
270
270
270
272
270
270
270
270
270
270
270
270
270
270
272
270
270
270
270
270
270
270
270
270
270

272
270
270
272
270
284
270
270
284
288
270
288
282
270
272
272
272
272
270
272
272
270
272
270
270
270
270
270
272
270

323
332
332
332
323
332
341
323
323
341
323
323
334
341
341
345
332
332
332
332
332
332
332
323
332
332
332
332
347
332

341
353
341
332
345
341
341
349
341
353
323
353
341
341
347
347
332
341
334
341
347
347
353
334
353
347
334
334
347
347

135
135
133
133
135
133
133
135
133
135
135
133
133
124
137
135
137
135
135
135
135
135
137
135
137
137
135
135
135
135

39

137
137
143
135
135
135
141
143
135
143
135
143
133
124
137
137
137
135
137
137
137
135
137
137
137
137
135
137
137
135

295
295
311
295
295
319
297
295
313
293
293
295
311
293
311
289
303
293
303
303
291
295
295
303
309
313
311
313
291
293

305
297
319
313
305
331
331
305
319
313
303
309
331
303
311
293
311
303
311
309
311
315
303
311
311
337
315
313
311
313

162
162
169
162
162
173
169
169
169
169
173
173
162
169
169
169
162
169
169
162
162
162
162
162
173
162
173
162
162
173

173
173
173
169
162
173
173
169
171
169
173
173
169
173
181
169
181
173
173
169
162
162
169
169
173
173
173
169
162
177

275
283
281
283
275
275
281
281
281
283
281
281
283
283
283
283
281
281
280
283
281
280
281
281
281
283
281
283
281
281

281
283
281
283
283
281
287
283
283
287
281
283
283
283
283
283
281
281
281
283
283
281
283
283
283
283
283
283
281
281

160
160
160
160
160
160
164
160
164
160
160
160
164
160
162
160
164
160
160
160
160
162
160
160
160
160
160
160
160
160

164
160
166
162
164
162
164
164
164
164
164
164
164
164
164
160
166
164
162
160
160
162
162
160
164
164
162
162
162
162

KG019
KG020
KG021
KG022
KG023
KG024
KG025
KG026
KG027
KG028
KG029
KG030
MA001
MA002
MA003
MA004
MA005
MA006
MA007
MA008
MA009
MA010
MA011
MA012
MA013
MA014
MA015
MA016
MA017
MA018

196
177
196
177
177
177
196
177
196
196
196
196
196
199
196
196
199
199
196
196
190
187
199
199
199
196
199
177
199
196

199
196
199
199
196
199
196
196
199
199
199
199
199
199
196
199
199
199
199
199
199
199
199
199
199
199
205
199
199
199

318
324
318
324
324
324
318
324
318
318
324
324
321
318
321
321
321
321
327
342
321
336
318
318
318
321
324
321
324
321

324
324
349
324
324
324
324
324
318
324
333
324
327
342
330
333
339
321
358
358
327
367
352
321
349
330
339
330
342
346

270
270
270
270
270
270
270
270
270
270
270
270
284
284
270
284
270
270
282
270
280
282
270
270
284
274
286
272
284
284

270
270
270
270
270
270
270
270
270
270
270
270
284
284
270
286
270
270
282
270
284
286
292
272
284
284
286
284
296
286

323
332
332
332
332
323
323
334
323
332
323
347
332
332
323
323
323
332
323
332
345
323
332
332
323
332
351
332
323
332

323
334
332
332
334
347
341
334
323
341
341
353
353
353
332
347
332
347
353
347
351
349
334
351
332
332
351
332
332
345

135
135
135
135
135
124
135
137
135
135
135
135
128
126
135
126
122
122
118
122
122
118
128
122
122
148
111
139
122
135

40

137
135
135
135
137
137
135
143
137
137
137
135
135
135
148
139
128
122
148
122
146
122
146
143
128
148
133
143
126
148

309
309
293
289
293
293
313
295
311
293
289
293
289
305
311
309
293
295
303
295
303
303
295
293
309
295
303
289
297
297

315
311
295
295
313
313
313
313
311
311
295
313
303
319
315
315
295
303
309
313
305
307
297
299
311
313
303
291
303
305

169
169
169
162
162
162
162
162
162
169
169
169
171
173
162
173
169
173
162
162
162
173
169
173
162
171
169
173
169
173

171
169
169
169
162
162
169
173
162
177
173
171
173
173
169
173
177
177
173
177
171
177
169
173
173
173
173
177
171
173

281
280
281
281
281
281
280
280
281
281
281
281
283
275
275
275
275
275
281
275
275
275
281
275
275
275
275
281
281
275

283
283
283
281
281
283
283
283
283
281
281
283
287
283
283
285
281
281
283
283
283
281
283
281
281
275
281
287
283
287

160
160
160
164
162
160
160
160
160
160
160
162
162
160
162
160
162
162
160
162
162
160
160
160
160
162
160
162
160
160

162
162
164
164
164
160
162
164
162
162
160
162
162
162
162
162
162
164
160
164
162
164
162
164
162
162
164
168
166
162

MA019
MA020
MA021
MA022
MA023
MA024
MA025
MA026
MA027
MA028
MA029
MA030
MA031
MA032
MA033
MA034
MA035
MA036
MN001
MN002
MN003
MN004
MN005
MN006
MN007
MN008
MN009
MN010
MN011
MN012

199
190
196
190
177
199
199
177
196
187
199
196
196
199
196
199
199
199
199
196
199
199
199
196
199
199
199
199
177
196

199
196
199
199
199
199
199
196
199
199
199
199
199
199
199
199
199
199
199
199
199
199
199
199
199
199
199
199
199
199

330
330
318
330
321
330
324
321
321
342
318
318
324
321
309
330
324
321
330
318
333
321
330
330
312
321
321
324
330
321

346
339
321
342
342
339
346
333
342
342
321
324
330
324
349
330
339
324
330
321
339
333
333
349
330
327
321
339
330
327

270
270
272
270
284
270
270
282
284
284
284
280
284
284
284
280
270
270
270
266
242
270
270
270
284
270
270
270
282
284

270
270
286
284
284
284
284
290
296
284
284
284
284
284
296
280
270
274
284
288
292
284
274
284
284
284
292
284
284
286

323
323
332
332
332
332
349
332
332
345
332
349
323
328
332
349
332
332
332
332
332
332
347
323
332
323
323
332
323
323

349
332
351
349
334
345
351
349
349
349
345
365
332
332
332
349
353
334
332
345
349
349
349
323
351
323
349
332
355
334

122
122
126
122
135
124
126
137
135
135
135
122
122
124
122
126
122
122
126
120
122
126
139
118
122
148
122
148
124
135

41

126
126
169
135
137
128
126
148
139
139
135
148
128
135
143
135
148
143
148
148
139
126
139
135
148
148
148
148
135
135

305
305
295
315
297
311
309
303
301
291
291
305
289
295
295
0
305
299
295
287
299
289
303
307
289
278
297
303
299
295

305
331
297
331
307
315
311
305
311
291
295
309
315
295
297
0
307
319
305
293
301
303
303
327
315
307
303
321
305
295

171
169
169
169
171
171
169
169
162
162
173
173
173
171
162
171
162
173
162
169
162
162
162
171
173
173
171
173
169
169

173
169
173
171
177
171
173
181
171
173
173
177
177
171
171
171
171
173
173
173
169
173
173
173
173
173
171
173
171
171

275
279
275
279
281
281
275
275
275
281
283
283
275
275
275
0
275
275
275
281
281
285
281
281
281
275
285
281
283
275

279
281
275
287
289
287
275
287
283
283
283
289
285
281
275
0
283
289
285
287
287
287
285
283
283
283
287
285
287
275

160
160
162
160
166
154
160
160
160
160
160
162
162
162
162
154
164
164
162
154
154
160
162
164
162
160
162
162
160
160

164
162
168
162
168
162
164
160
168
160
168
162
162
162
166
164
164
164
162
160
160
164
164
164
164
162
162
168
160
162

MN013
MN014
MN015
MN016
MN017
MN018
MN019
MN021
MN022
MN023
MN025
MN026
MN027
MN028
MN029
MN030
MN031
MN032
MN033
MN034
MN035
MN036
MN037
MN038
MN039
MN040
MN041
MN042
MN043
MN044

196
199
196
196
196
199
199
196
199
199
196
196
199
199
196
196
199
196
199
199
196
199
199
196
199
199
196
177
196
199

199
199
199
199
196
199
199
199
199
199
199
199
199
199
199
196
199
196
199
199
199
199
199
199
199
199
199
199
199
199

321
330
318
330
309
321
321
321
318
318
321
318
321
333
318
333
318
321
330
318
321
324
324
342
333
321
321
330
321
318

324
330
349
333
358
330
330
333
318
318
333
324
333
333
321
361
361
339
330
355
339
342
333
364
364
352
333
330
342
339

284
270
284
284
272
284
270
284
272
270
284
270
270
272
284
270
282
274
282
270
272
270
272
284
286
270
272
270
272
280

290
274
284
296
290
284
272
284
284
272
284
286
296
296
294
284
292
284
282
284
290
296
284
290
290
272
284
274
284
284

345
347
332
323
332
323
323
323
323
332
345
323
332
332
323
323
328
332
349
332
323
323
323
323
323
323
345
332
323
323

349
351
332
332
353
323
323
345
332
349
349
323
332
332
332
332
349
345
349
345
332
349
323
349
332
323
359
332
332
349

135
126
126
122
122
124
135
122
126
135
128
124
135
135
122
122
126
111
122
124
124
124
111
126
126
135
122
126
122
137

42

135
126
150
148
126
126
135
135
148
148
154
124
135
148
135
143
148
137
124
126
137
148
128
126
135
148
148
148
126
154

321
303
313
305
295
293
293
311
293
293
297
305
293
293
315
303
305
291
287
307
301
289
291
0
293
293
293
297
278
301

323
315
315
307
305
303
327
323
301
293
303
321
315
305
321
319
321
291
327
313
301
289
297
0
295
305
297
305
303
303

162
162
173
171
169
162
162
169
162
162
162
169
171
162
171
169
162
171
171
171
169
173
169
162
162
162
173
173
171
162

171
169
177
173
171
171
171
173
171
173
162
177
173
171
171
173
177
175
173
173
169
173
171
169
169
169
179
173
173
169

281
275
275
275
275
287
281
283
281
281
275
275
281
275
281
283
275
275
275
283
275
281
281
283
281
281
275
281
275
275

285
283
287
283
281
289
287
287
281
281
275
285
287
287
283
287
287
287
283
285
289
285
287
289
289
283
281
281
287
283

160
162
160
160
160
160
160
162
162
154
160
160
162
160
160
160
162
164
160
162
162
160
160
162
160
160
160
160
160
160

160
164
160
164
164
162
160
168
168
164
160
164
164
160
162
166
162
168
168
162
168
162
162
162
162
160
164
162
166
160

MN045
MN046
MN047
MN048
MN049
MN050
MN051
MN052
MN053
MN054
MN055
BE001
BE002
BE003
BE004
BE005
BE006
BE007
BE008
BE009
BE010
BE011
BE012
BE013
BE014
BE015
BE016
BE017
BE018
BE019

199
196
187
199
199
196
196
196
199
199
196
199
196
199
199
196
196
199
187
177
199
199
199
196
199
177
177
196
187
196

199
199
199
199
199
199
199
196
199
199
196
199
199
199
199
199
199
199
196
187
199
199
199
199
199
199
199
199
199
196

318
318
318
321
330
318
309
318
321
330
321
321
321
321
318
318
321
321
330
321
318
330
330
330
321
327
318
318
318
318

327
330
339
358
330
321
367
324
321
333
321
330
321
330
367
339
330
349
330
321
336
330
349
346
324
342
342
330
339
355

284
274
272
270
270
286
270
284
272
272
270
284
270
272
270
272
270
270
270
284
270
270
270
270
280
284
272
270
270
272

284
292
272
292
294
290
272
296
284
292
284
284
270
284
272
292
272
284
270
284
284
270
274
272
280
296
290
270
272
272

323
349
332
323
332
332
323
323
349
332
349
332
323
332
323
323
332
323
323
323
332
323
323
323
332
323
334
349
332
334

332
351
349
334
345
353
351
332
353
345
349
349
332
353
353
332
332
323
349
323
353
349
334
332
347
332
345
355
349
349

122
122
124
135
126
124
139
122
122
124
126
122
126
122
126
111
122
122
122
126
126
135
122
135
122
135
118
113
148
135

43

126
148
126
148
135
126
158
126
122
135
154
122
135
148
135
137
141
156
158
130
128
135
146
148
135
148
135
148
148
135

293
297
295
297
297
305
297
305
293
289
303
297
293
295
289
289
293
295
291
291
293
295
295
303
313
297
289
293
305
0

307
303
297
313
297
305
297
309
297
305
315
313
297
295
321
303
299
303
305
297
301
295
295
305
323
303
293
305
327
0

171
171
162
162
162
162
162
162
162
162
162
162
173
171
169
169
162
177
169
162
173
162
162
169
162
162
162
169
169
162

173
173
171
162
171
169
177
169
171
173
173
171
173
177
173
173
171
179
169
173
173
171
169
177
173
171
171
171
169
177

275
281
281
281
283
283
281
283
281
281
275
281
281
275
275
275
275
275
283
275
281
275
281
281
275
275
281
275
275
275

283
285
281
283
287
283
281
285
287
281
281
281
281
281
281
289
285
275
283
275
281
289
285
289
289
281
281
285
275
283

160
154
154
154
154
160
160
160
160
160
160
162
160
162
164
160
164
162
162
160
162
156
156
162
162
162
160
160
160
168

162
160
162
160
162
162
162
162
164
164
162
162
162
164
168
162
166
166
162
164
162
160
168
162
164
162
164
162
162
168

BE020
BE021
BE022
BE023
BE024
BE025
BE026
BE027
BE028
BE029
BE030
BE031
BE032
BE033
BE034
BE035
BE036
BE037
BE038
BE039
BE040
NA001
NA002
NA003
NA004
NA005
NA006
NA007
NA008
NA009

177
199
196
199
199
199
196
199
199
177
196
177
196
199
177
199
199
177
199
187
199
199
199
199
196
177
199
199
196
196

199
199
199
199
199
199
199
199
199
198
199
199
199
199
199
199
199
196
199
199
199
199
199
199
199
177
199
199
199
199

327
336
318
330
324
330
336
330
339
324
318
327
324
330
318
318
318
321
330
327
318
321
336
324
321
324
321
324
321
318

330
342
324
355
339
346
364
358
339
327
330
346
352
330
324
339
324
346
339
330
318
352
342
324
324
352
321
330
346
321

280
270
274
284
274
270
270
270
270
270
272
270
242
272
272
272
272
284
280
272
270
272
270
270
280
270
272
274
242
270

280
282
284
292
286
284
284
274
272
270
284
270
270
290
272
280
272
290
292
292
270
274
284
272
290
286
286
274
270
272

323
323
332
332
332
323
332
323
323
332
323
332
332
332
332
323
332
323
332
351
323
332
323
323
341
341
334
347
332
323

323
323
345
332
351
332
332
349
332
351
332
351
351
332
349
351
332
332
359
353
351
349
332
323
351
349
347
353
345
332

122
120
126
126
143
120
135
135
122
122
120
122
122
124
122
124
122
122
135
111
135
122
122
122
133
135
124
135
124
137

44

133
148
126
126
148
135
135
156
139
135
137
139
135
148
137
135
122
135
137
122
137
126
135
135
135
137
143
154
124
146

283
291
287
295
299
289
297
291
303
291
295
297
297
301
289
0
297
295
289
293
289
295
295
295
297
305
289
295
293
311

305
303
327
315
303
307
299
295
305
305
297
305
301
323
305
0
305
303
289
305
323
295
313
315
307
323
299
295
301
317

162
169
173
171
171
171
169
162
169
173
162
162
162
169
169
162
162
162
171
169
162
169
162
162
169
162
162
171
162
169

173
173
173
173
171
171
171
169
169
173
169
171
173
177
171
171
171
173
175
177
173
173
169
173
169
169
173
177
171
173

281
281
275
283
275
275
283
275
275
275
275
275
275
275
275
281
281
281
283
275
283
283
283
281
287
287
283
283
275
281

283
283
283
287
283
275
283
283
289
281
283
283
275
283
281
281
281
281
287
283
283
283
289
283
289
289
287
285
281
287

160
160
160
154
162
160
160
160
160
162
160
162
160
160
160
160
160
154
160
162
162
160
162
160
162
162
160
164
154
162

162
162
162
162
166
166
160
160
160
168
164
164
164
162
162
162
162
160
162
164
164
162
164
164
164
164
164
164
164
164

NA010
NA011
NA012
NA013
NA014
NA015
NA016
NA017
NA018
NA019
NA020
NA021
NA022
NA023
NA024
NA025
NA026
NA027
NA028
NA029
NA030
NA031
NA032
NA033
NA034
NA035
NA036
NA037
NA038
NA039

195
177
196
196
177
196
199
199
199
177
199
199
199
177
199
177
196
199
199
199
199
177
177
177
177
196
177
199
199
199

198
196
196
196
199
199
199
199
199
199
199
199
199
199
199
199
199
199
199
199
199
199
199
177
199
199
199
199
199
199

330
321
321
321
321
349
327
339
321
361
321
318
321
318
318
321
321
321
321
318
321
336
321
324
318
321
318
336
318
318

346
321
327
321
324
352
352
358
373
373
324
318
342
330
324
330
321
324
327
333
321
355
349
358
321
321
321
352
321
349

272
270
270
270
270
290
270
270
270
284
270
270
270
270
270
242
270
284
270
270
272
270
270
270
270
284
284
242
270
270

272
270
284
292
270
292
272
272
290
292
272
272
282
272
270
272
284
294
274
270
292
284
272
270
274
284
284
270
272
294

332
323
332
332
332
323
323
323
323
323
332
345
347
323
323
332
323
323
323
323
347
323
323
323
341
323
323
323
334
347

332
332
332
349
332
353
334
323
323
323
334
345
351
334
351
332
347
347
323
341
349
345
349
332
353
351
349
349
349
347

102
124
113
137
135
122
139
135
133
122
122
104
122
135
128
113
133
135
124
135
135
122
133
133
122
133
135
122
133
135

45

104
137
135
146
137
146
146
135
146
146
137
124
124
137
135
137
143
135
137
154
135
135
135
135
124
143
146
124
133
146

295
319
299
283
295
293
293
293
293
295
295
0
307
299
311
295
293
289
0
289
0
293
295
295
295
305
295
291
293
293

297
321
321
321
323
293
295
293
295
295
295
0
309
311
329
305
293
295
0
293
0
319
295
309
309
319
305
293
295
303

169
162
171
173
169
171
169
171
162
169
169
0
162
162
162
169
162
171
162
162
171
171
162
171
162
169
173
169
171
162

188
177
173
177
171
177
171
173
169
177
169
0
162
169
173
177
171
177
173
177
171
173
169
177
177
177
173
171
171
162

269
269
275
281
275
279
283
275
275
275
285
0
275
275
281
283
283
275
281
285
285
275
283
283
287
285
283
285
283
275

271
271
281
289
281
283
289
283
287
283
289
0
287
289
281
289
289
287
281
287
289
281
289
287
287
285
289
285
289
275

156
164
164
164
162
160
162
162
154
154
162
156
160
154
160
160
160
160
162
160
160
164
160
162
160
164
160
164
164
164

168
166
168
168
168
164
164
164
166
162
166
160
164
162
162
164
164
164
166
162
162
164
160
164
160
164
162
164
166
164

NA040
NA041
NA042
NA043
NA044
NA045
NA046
NA047
NA048
NA049
NA050
NA051
NA052
NA053
NA054
NA055

196
199
196
199
199
199
196
196
199
177
196
190
177
199
196
199

199
199
199
199
199
199
211
199
199
196
196
211
199
199
199
211

318
321
321
321
321
321
321
318
321
330
318
324
324
321
318
346

318
324
352
321
321
370
321
321
321
358
321
330
330
355
333
358

270
270
270
270
270
272
270
270
270
270
272
270
282
270
284
270

286
292
284
274
272
284
272
272
270
290
272
272
284
270
284
272

323
323
347
332
334
349
323
323
323
347
332
323
323
332
332
345

332
332
353
353
349
353
323
351
334
351
349
323
332
332
332
351

122
120
135
124
122
128
109
135
122
137
135
135
124
122
122
124

46

135
135
137
135
143
135
109
137
124
152
154
135
137
139
135
135

295
305
299
293
295
289
295
293
297
295
303
295
293
293
299
303

295
329
311
299
305
311
305
295
305
321
311
311
311
295
313
313

162
173
171
169
179
173
169
169
171
171
173
169
169
162
173
177

173
177
173
169
179
177
177
171
177
177
177
177
173
162
179
179

285
281
283
283
283
281
287
289
283
283
275
281
283
275
281
281

289
287
283
285
287
289
289
289
289
283
289
283
289
283
283
283

160
160
164
160
162
164
160
164
162
160
160
148
160
154
160
164

162
160
166
166
164
164
164
166
168
160
160
166
164
162
164
164

47