Installing BD FACSDiva Software

Use the following instructions to install BD FACSDiva software or upgrade to the latest version. The installation CD is packaged with the BD FACSDiva software Getting Started guide or BD FACSCanto system software. NOTICE You must have Microsoft Windows Administrator access to install BD FACSDiva software. Please read all instructions before you proceed. NOTICE Only the US English version of the Microsoft Windows XP operating system is supported by BD FACSDiva software version 6.0. If you are installing the software for the first time, skip to Installing New Software on page 2. Otherwise, continue with the next section. NOTICE Once installation is complete, see the Cytometer Setup and Tracking Application Guide to learn how to create base configurations.

Before upgrading the software, do the following: 1 In the current version of software, select the cytometer configuration that
will be used as the base configuration for Cytometer Setup and Tracking. The number of lasers, detectors, and parameters that are associated with that configuration will be used to populate the new base configuration. For details, see the Cytometer Setup and Tracking Application Guide.

2 It is important that the delays for all available lasers be properly set prior to
upgrading. Refer to the users guide for your particular cytometer. Correct laser delays are used to determine the proper laser order for cytometer configuration in Cytometer Setup and Tracking.

3 Make sure you have a valid database backup stored off the computer hard
disk (eg, on a server or CD/DVD). Refer to the BD FACSDiva Software Reference Manual for instructions.

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4 Workstations must be XW4100 or later. Update your operating system to

Windows XP, Service Pack 2 (SP2). Order or download service packs from the Microsoft website (www.microsoft.com/downloads).

5 BD recommends that you defragment the hard disk before you install new
software (the C and D drives).

Installing New Software

The installer places the following components on the hard drive. If the correct version of a helper application (not including the main BD FACSDiva software) is already installed, the installer skips to the next installation step.
BD FACSDiva software 6.0 BD FACSDiva Data Manager Sentinel System Driver v5.41.1 Microsoft .NET 2.0 Framework Java 2 Runtime Environment (JRE) v1.5.0_11 Sybase SQL Anywhere v9.0.2 Adobe Acrobat Reader v7.0

NOTICE For computers running the BD FACSAria or the BD LSR II cytometers: area scaling, window extension, and laser delay values are stored in the database. If you plan to install an empty database, record these values before uninstalling the software so you can re-enter them later.

1 Before installation, turn the flow cytometer power off and then on again.
Wait 5 minutes and restart the computer.

2 Close all open applications and windows. 3 Insert the BD FACSDiva installation CD into the CD-ROM drive.
NOTICE If a previous version of the BD FACSDiva software application is installed, the uninstall process removes that version and its associated files while preserving the database and list-mode data files.

Installing BD FACSDiva Software

If the installer does not start automatically after uninstalling the previous version, use Windows Explorer to view the CD contents, then find and double-click the Setup.exe icon or remove and reinsert the CD.

4 Carefully review the ReadMe file. Click

to continue with installation.

NOTICE The ReadMe file contains important software information that is not included in the accompanying documentation. Tip BD Biosciences recommends that you print the ReadMe file and place the printed copy with your Getting Started guide or cytometer manual for reference. To locate ReadMe information after software installation, double-click the shortcut on the desktop.

5 When the welcome screen appears, click Next.

6 Click Yes to accept the license agreement and continue installation. 7 Verify the destination folder. Click Next.

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By default, the software is installed in the Program Files\BD FACSDiva Software folder on the C drive.

8 In the cytometer selection window, select the checkbox for your flow
cytometer and click Next (Figure 1-1).
Figure 1-1 Selecting a cytometer option

For offline workstations, select the cytometer used most often in your laboratory.

Installing BD FACSDiva Software

9 For the BD LSR II or BD FACSCanto flow cytometer, select the option

that corresponds to your cytometer, and click Next.
Figure 1-2 BD FACSCanto cytometer options (example)

10 When prompted, select a database option, and click Next.

Select Existing database (Recommended) to continue working with data in the current database. The database will be upgraded to work with the new software version. Only select New empty database from the install media if you want to install an empty database. The existing database will be renamed BDFACS.dbx, where x is the next consecutive number.

NOTICE Contact BD Biosciences Customer Support before upgrading with a new empty database on an acquisition workstation.

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11 Wait while the installer loads software. (This can take several minutes.)
The installer loads BD FACSDiva software and its support files in the appropriate locations. If the workstation is connected to a cytometer, the installer uploads files to the cytometer. While the installer is checking to see if the workstation is connected to a cytometer, the following message appears on the screen:
Figure 1-3 Message for BD LSR II (example)

Do not click the mouse or press any keys while the DownloadVxWorks message is displayed. Doing so could cause the installer to lock up and prevent installation from continuing.

Installing BD FACSDiva Software

If the VxWorks download is unsuccessful, the following message appears:

If you are installing the software on an analysis-only workstation, click No. The VxWorks download is not required. If you are installing the software on an acquisition workstation, verify that the cytometer is turned on and connected to the workstation, and then click Yes to try the VxWorks download again. If the same message appears again, click No, finish the installation, and contact BD Biosciences Customer Support. Do not run your flow cytometer until VxWorks has been successfully installed.

12 The Reboot Cytometer reminder message appears. Click OK to close the

message.

13 Select Yes to restart the computer immediately after installation. Click

Finish to complete the installation.

14 Once the computer restarts, install the security module in the USB port of
the computer workstation, if needed. The security module must be in place to run BD FACSDiva software. The security module can be installed in any USB port.

15 Turn the cytometer power off and then on again. Wait 5 minutes before
launching the BD FACSDiva software.

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To finalize the download of cytometer files, you must restart the cytometer after the software is upgraded and the computer has been restarted. The update will be complete when you launch the new software version and establish connection with the cytometer. Do not interrupt the application during startup. NOTICE If you plan to use the Cytometer Setup and Tracking features to create base configurations, see the Cytometer Setup and Tracking Application Guide.

Adjusting Video Acceleration Settings

If you are running the HP XW4100 or HP XW4200 on a Windows XP computer system, you need to adjust video accelerator settings to run this version of BD FACSDiva software. For other computer systems, your settings might need to be adjusted if you notice that BD FACSDiva software is very slow to respond to mouse clicks and menus are very slow to appear. If you are not sure, contact your local BD Biosciences service representative for assistance.

1 Right-click on your computer desktop and choose Properties. 2 Click the Settings tab.

Installing BD FACSDiva Software

3 Ensure the Color quality option is set as shown and click the Advanced
button.

single monitor

dual monitors

4 Click the Troubleshoot button.

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5 Move the Hardware acceleration pointer to the fourth tick mark down
from the Full setting:

6 Click Apply, and then OK. 7 If you have a second monitor, choose the other monitor from the Display
menu and repeat steps 3 through 6.

8 Click OK to close the Display Properties window.

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Installing BD FACSDiva Software