Process

Research Development

Development Services
Everything you need –
at every stage of development

www.safcbiosciences.com Member of the Sigma-Aldrich Group

 Taking the guesswork out
of cell line development

Trust SAFC Biosciences to accelerate the

pre-clinical stages of your development processes.

Molecular Biology Consultation Cell Xpress™ Clone Selection

and Services
Absolute: Analyze, select, document
Wisdom: Choose SAFC Biosciences and perpetuate only the clones that
and expect high performance products, meet your development criteria in
services and information – all designed just weeks, cutting cell line
to enable you to quickly select the development timelines in half.
best possible option to meet your
• Technology for single-cell cloning
research goals.
of the most productive clones in
• Expression feasibility a population
- Secretion assessment - Increase specific productivity
and titer
- Expression strategies
- Stabilize cell line genetic drift
• Transfection optimization
- Create clones that are more
- Improved methodology
uniform in productivity
- Optoinjection

Extend your capabilities with a full

line of development services

Molecular Media
Scale-up &
Biology Optimization Process
Validation
Consultation and Development
Studies
and Services Adaptation

Gene time
of
Interest

Clone
Selection Stability Process
Assessment Feasibility Product
(Cell Xpress™)
Responsive Expertise
SAFC Biosciences is the world’s leading supplier
of cell culture reagents to producers of marketed biological
products. We are committed to supplying high-quality
critical raw materials and specialized cell culture products
and services to the global health care industry.

By providing the broadest range of highly customized

products and services possible to cell culture-based
manufacturing companies we demonstrate our commitment
to being responsive experts.

Cut cell line Facilitate

development in decisions with
half with feasibility analysis
Cell Xpress™

Meet your
research goals

Add control to your

biological processes

Benefit from Achieve consistent

35 years' development results
experience in media through stability
optimization assessment
 Increase your specific productivity and titer with
Cell Xpress™ Cell Line Development

Parental Cell Line Cell Xpress™ clone

Commercial cell line pre-Cell XpressTM Commercial cell line 50 doublings post-Cell XpressTM

Medium Optimization and Adaptation Stability Assessment

Proven: Benefit from over 35 years’ Consistent: As your chosen
expertise in successfully optimizing manufacturer and supply partner, we
media component composition for understand that material and processes
increased productivity and overall must be carefully and proactively
robustness in the cell culture managed to ensure consistent
process. performance and manage
product risk.
• Statistical Approaches
• Cell line stability
- Mixture experiments
- Generational consistency studies
- Factorial matrices
- Visual analysis of clone
- Central composite designs
performance at any stage
• Rational Design Approaches
• Media stability
- Spent medium analysis (amino
- Fresh and spent media analysis
acids, glutamine, lactate, etc.)
- Experience with serum-free,
- New assay development (vitamins,
animal-component free,
fatty acids, biomarkers, trace
protein-free and chemically
elements, peptide mapping, etc.)
defined media formulations
• Component analysis

Using our computer-aided cell screening, photographic analysis,

laser-based selection and rapid media optimization techniques,
you will find we remove any need for guesswork.
Process Development Scale-Up and Validation
Powerful: Harness our scientific, Simple: SAFC Biosciences adds
technical and commercial knowledge control to biological processes
for scaleable, application-tested cell through customized projects and
culture solutions for expression of investigations of all potential sources
your proteins. of performance or quality variation.
• Bioreactor scale feasibility • Extensive library of cell culture
products
- Process control
- Consistent performance
- Development of bioreactor
processes - Scaleable
- Feed strategies • Quantification of components in
fresh or spent media
- Value-added feeds/supplements
(optimized hydrolysates) • Monitoring productivity during
process optimization
- Contract projects
• Characterizing product quality
• Feed development
• Customized assays
- Amino acids
• Upstream & downstream
- Vitamins
biological buffers
- Hydrolysates
- Productivity inducers
• imMEDIAte ADVANTAGE™
- 10-day turnaround on custom
media formulations
- Standardized raw materials
- Mirrors manufacturing methods
in large-scale cGMP facilities

Facilitate decisions with

feasibility analysis
Contact us

United States

SAFC Biosciences, Inc.

13804 W. 107th Street
Lenexa, Kansas 66215
USA

Tel: +1 913-469-5580
Toll Free – USA: 1 800-255-6032
Fax: +1 913-469-5584
E-mail: info-na@sial.com

Europe

SAFC Biosciences Ltd.

Smeaton Road, West Portway
Andover, Hampshire SP10 3LF
UNITED KINGDOM

Tel: +44 (0)1264-333311

Fax: +44 (0)1264-332412
E-mail: info-eu@sial.com

Asia Pacific

SAFC Biosciences Pty. Ltd.

18-20 Export Drive
Brooklyn, Victoria 3025
AUSTRALIA

Tel: +61 (0)3-9362-4500

Toll Free – AUS: 1 800-200-404
Fax: +61 (0)3-9315-1656
E-mail: info-ap@sial.com

imMEDIAte ADVANTAGE™ is a
trademark of SAFC Biosciences, Inc.

Cell Xpress™ is a trademark of Cyntellect.

© 2007 SAFC Biosciences, Inc.

Issued January 2007 G055

www.safcbiosciences.com Member of the Sigma-Aldrich Group