MicroBioSys

A Unique Programme and Network

MISSION AND VISION Our mission involves increasing the bio-potential of bacterial treatments significantly, boosting yields and reducing the amount of required chemical fertilizers used in plant cultivation. We envisage producing a superelite bacterial product in local propagating units in the framework of a multifunctional service network.
PROBLEM AND SOLUTION

High product prices and lack of development greatly hinder the spread of bacterial products and their effective application in agriculture. Also resulting from high costs application methods are unfavourable as well (far too low doses, single treatment, low number of bacterial species). The price of bacterial product (SYMBION) made by applying the new propagation technology of MicroBioSys (MBS) is markedly lower compare to the products also available on the market. This lower price will remove the stopping force and will at the same time allow for an explosive expansion both on the local and international markets. MBS is an integrated bacterisation system; the first on the world encompassing the following determining factors in a complex way: New bacterial propagation technology Low production cost New type of bacterisation with 100 -fold doses and repetitive application Our own, superselected N-fixing bacterial strains Bacterial products adapted to local conditions NEW BACTERIAL PROPAGATION TECHNOLOGY The main restrictive factors of producing and applying bacterial products are producing them expensively at central locations, transporting large quantities of ballast water at high costs and using uniform products in various fields of application. The product, propagation technique and application around which MBS developments are centred involve producing inocula locally, in large quantities and at low costs. Within this system, while the basic inoculum is produced centrally, the specialised bacterial product is produced in fermentors (propagatory units) developed by MBS itself at the places of application. With the help of this multi-stage system (production in 3 steps) the quantity of bacterial products produced in local propagatory units could be several times as much as the annual output of a large, conventional bacterium-fertiliser producing factory. The number of local propagators can be increased on demand. MBS will make its propagatory unit and technology available to every large-scale customer joining the system as a partner. The propagating units in the network are also the basic units of the regional network and communication with the system.

Local improvement in a global network

LOW PRICE As compared with current practice the favourable price offered by MBS allows for a more effective bacterisation process with more reliable results. Within the framework of the MBS system, the unit cost of the 100-fold quantity of bacteria per unit area is low, which makes a significant increase in the biological potential of plant cultivation possible. SYMBION - SUPERELITE BACTERIAL PRODUCTS In creating our product - named SYMBION - we utilise selected nitrogen-fixing bacteria. With the regular mass application of our bio-products, chemical nitrogen fertilisers will become replaceable in 7075, or even 100 %. By decreasing the application of chemical fertilisers not only production costs can be reduced, but also the biological condition of the soil will improve markedly (decreasing nitrate contamination, increasing humus content). On the other hand, using SYMBION with fertilizers, the beneficial effects of bacteria will increase the yields in conventional agricultural practice. MBS products, besides nitrogen-fixing microorganisms, may also contain phosphorus-resolving, growth-stimulating bacteria and/or ones providing protection against pathogens and pests. The advantage of the new technology is that it is possible to develop and produce the most effective bacterial product to suit local conditions.

NETWORK DEVELOPMENT MBS products are made in a network of propagation units. This network offers help to growers in selecting the right type of plants, in planning and organising production and in selling the end-product. Network members are entitled to use all available services of professional consultation, informationprovision and distribution networks. INTELLECTUAL PROPERTY RIGHTS Patents protect our intellectual interest: for the product, propagation technology elements and for MicroBioSys hyper network, as well as for the technology of processing bacterial products so as to achieve effective and close plant-bacterial relationships in a controlled way. We started protection for propagation units (including bacterial-fermenting units within mobile container) applied, which is suitable for the mass propagation of microorganisms. In consequence of recent developments we are to submit further applications for patents in the near future.

Local improvement in a global network

APPENDIX 1

SCHEME OF THE MBS PRODUCTION SYSTEM

I.
Central inoculum producer Responsibility: Providing and developing the basic bacterial culture and agents Systems management, services
Additive I. Local improvement in a global network

1 litre

II.
Regional aerobic propagator Responsibility: First-stage aerobic propagation of the basic bacterial culture: Additive I + water The regional centre of the network 20 litre

Additive II.

III.
Local propagation units Responsibility: Making the end-product propagating aerobic, anaerobic microorganism: Additive II + water The local centre of the network 1000 litre

End-product SYMBION

BACTERIZATION with the quantity upto 1,000 l/ha/year (treatment of seed-beds, seeds, stands and stables)
Increasing soil bio-potential Improvement in soil quality By Increasing humus content Nitrogen fixation and Agents Increasing plant bio-potential Improved nutrient supply, Growth stimulation, Fe, micronutrient supplement Yield increase and steppedup growth Plant protection Extra protection against Bacteria, Fungi and Insects Induced and active protection Increasing crop bio-potential Due to improvement in Composition (vitamin, amino acid content) and Market value (e.g. bioproduct) Increase in crop value

Co

Cellulose decomposition

APPENDIX 2
PILLARS OF VALUE GENERATION WITH MBS guaranteeing the permanent presence of useful microorganisms increasing the bio-potential of plant cultivation (re)vitalising and restoring soil life creating healthy microbial environment to plants in the interest of stepped-up protection and yields increasing and providing nutrient supply for plants applying large quantities of beneficial microbial bio-mass applying large number of selected microorganisms to generate extra effect creating and introducing cost-effective production and application facilitating related production and sales safety. ongoing R & D COMPARISON OF CONVENTIONAL BACTERIAL GRTILIZERS AND SYMBION Conventional bacterial fertilizers 2(-3)

SYMBION
! 23

No. of beneficial N-fixing bacterial species

Local improvement in a global network

Applied quantity litre/ha/year Germ count germs/ml Bacterium used billion (1012) germs/ha/year Bio-active material applied, kg/ha/year Product price USD/ha/year Unit price of end product cent/litre

10 109 10 ~ 0.1 ~ 18 ~ 180

! ! ! ! !

to 1000 109 1000 ~5 ~ 20 ~2

WHAT'S GOOD IN MBS? 1. MBS is the only system to offer the repetitive and mass application of useful microorganisms, which is necessary to achieve a sustained increase in the biological potential, 2. it provides the consumer with an almost 100-fold biological value for a comparable price, 3. a product best meeting the local application requirements and soil-conditions can be created within the framework of MBS, 4. the system and its operation is easy to control and realise at any place, 5. alongside product development the system's information, marketing etc. networks are also developing, which will lead to an added value in supplementary services, 6. this knowledge-intensive system is easy to expand and export.

Additional information about SYMBION use in the practice

Local improvement in a global network

Effect of SYMBION on number of useful bacteria in the soil

SYMBION treatments No. of bacteria

Hygh and durable bacteria level in soil using SYMBION treatments

No.of bacteria at traditional bacterial products by one treatment time Plant vegetation period Stubble field, sowing

MBS

Local improvement in a global network