Monday Test 3.

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Evening Batch (rDNA , Microbial Physiology,)

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A researcher needs to clone a sequence of part of a eukaryotic genome in order to express the sequence and to modify the polypeptide product. She would be able to satisfy these requirements by using which of the following vectors? A. a bacterial plasmid B. BAC to accommodate the size of the sequence C. a modified bacteriophage D. a YAC with appropriate cellular enzymes

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Which of the following best describes the complete sequence of steps occurring during every cycle of PCR? 1. The primers hybridize to the target DNA. 2. The mixture is heated to a high temperature to denature the doublestranded target DNA. 3. Fresh DNA polymerase is added. 4. DNA polymerase extends the primers to make a copy of the target DNA. A. 2, 1, 4 B. 1, 3, 2, 4 C. 3, 4, 1, 2 D. 3, 4, 2

 A linear DNA fragment is (100%) labeled using flurochrome at one end and has 3 restriction sites for Hind III. If it is partially digested by Hind III so that all possible fragment are produced. When the partially digested product were separated using agarose gel electrophoresis, How many of these fragment will be labeled and how many not be labeled? A. 6 unlabeled, 4 labeled B. 4 unlabeled, 4 labeled C. 4 unlabeled 3 labeled D. Cannot be determined

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B)

C)

D)

E)

The segment of DNA shown in above has restriction sites I and II, which create restriction fragments A, B, and C. Which of the gels produced by electrophoresis shown below best represents the separation and identity of these fragments? A B C D

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A. B. C. D. E.coli strain used in Transformation techniques is E.co R alpha E.co T alpha E.coli DH5 alpha E.coli Th5 alpha

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Calculate the value of x in the following question. 1/0.005 1/x = 100 . A. 0.1 B. 0.01 C. 0.001 D. 0.005

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A. B. C. D. Which of the following contains on singly copy of a gene? Liver cell in Liverworts Heart inner cell layer Outer layer of Bastoderm Nucleus of frog egg

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A. B. C. D. Which of the following is not an X linked disorder? Xeroderma Pigmentosa Muscular Dystrophy Colourblindness Fraby Disease

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Your PI gives you a bacterial culture with population density of 250 CFU/ml. A bacterium divides every 40 minutes. Assuming that the cells are in the exponential phase of growth, what is the count of cells you will have after 4 hours? A. 2.0 * 104 CFU/ml B. 0.5 * 106 CFU/ml C. 1.6 *104 CFU/ml D. 2.0 * 106 CFU/ml

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Which of the following is not a mechanism for uptake of nutrients by microorganisms? A. Uptake of nutrients along the concentration gradient utilizing energy B. Requirement of a carrier to support transport along the concentration gradient C. Chemical modification of solute during its uptake D. Uptake against the concentration gradient

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The mean growth rate constant (k) of a yeast species is 0.21h-1. What is the species' doubling time (g) in hours? A. 3.5 hours B. 4.8 hours C. 5.0 hours D. 1.5 hours

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A new isolate of a facultative anaerobe utilizes either oxygen or Pyruvate as terminal electron acceptor. This bacterium was grown first anaerobically with glucose as the sole carbon source, then aerobically with lactose as the sole carbon source. Net increase in ATP production per mole of the carbon source, when the culture was switched from 1st medium to the 2nd medium, would be A. 2-fold B. 4-fold C. 19-fold D. 38-fold

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A growing bacterial culture with a doubling time of 20 min reaches cell density of 2 * 108 cells/ml in 3 hours. How much time would it take to reach the cell density of 1 * 108 cells/ml? A. 200 min B. 180 min C. 160 min D. 90 min

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100 bacteria were inoculated into a nutrient rich medium. It was found that at the end of 10 hours the total number of cells is 105. What is the number of generations that have passed in this time and the generation time respectively? A. 10, 120 minutes B. 10, 60 minutes C. 20, 30 minutes D. 40, 15 minutes

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A culture of Bacteria, have doubling time of 20 min when cultured on Glucose and 40 min when cultured on Lactose. What will be the doubling time, in the second half of the growth period? A. 20 min B. 60 min C. 40 min D. 30 min

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A. B. C. D. What is the square root of 69169 262 263 268 269

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A Linear DNA fragment has restriction sites for BamHI. When completely digested, we got 6.4 , 4.1 and 1.0 fragments. When partially digested , we got 10.5., 7.4, 6.4, 4.1 and 1.0 fragments. What is the order of the restriction sites on the linear DNA. ? A. 4.1, 6.4, 1.0 B. 6.4, 4.1, 1.0 C. 1.0, 4.1, 6.4 D. Cant be determined

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There are 3 persons Sudhir, Arvind, and Gauri. Sudhir lent cars to Arvind and Gauri as many as they had already. After some time Arvind gave as many cars to Sudhir and Gauri as many as they have. After sometime Gauri did the same thing. At the end of this transaction each one of them had 24. Find the cars each originally had. Sudhir had 39 cars, Arvind had 21 cars and Gauri had 12 cars. Sudhir had 29 cars, Arvind had 11 cars and Gauri had 120 cars. Sudhir had 21 cars, Arvind had 39 cars and Gauri had 12 cars. Sudhir had 39 cars, Arvind had 12 cars and Gauri had 21 cars.

A. B. C. D.

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Which of the following is larger than 3/5? (1) (2) 39/50 (3) 7/25 (4) 3/10 (5) 59/100

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It takes Mr. Karthik y hours to complete typing a manuscript. After 2 hours, he was called away. What fractional part of the assignment was left incomplete? Y+2 Y+2/Y Y-2 Y-2/Y

A. B. C. D.

Result
1 2 3 4 5 6 7 8 9 10 D A A D C B D A C A 11 12 13 14 15 16 17 18 19 20 B D C B C B A A B D