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  • CRD Subsample

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    Fahmy Abo Walid Kseibi
    1K vizualizări34 pagini
    Subsampling experiments promote efficient use of space and materials. They are most efficient when there is much variation in response among explants. It reduces the df for experimental error which means a higher F-value is required to detect significant differences among treatments.

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    Subsampling experiments promote efficient use of space and materials. They are most efficient when there is much variation in response among explants. It reduces the df for experimental error which means a higher F-value is required to detect significant differences among treatments.

    Drepturi de autor:

    Attribution Non-Commercial (BY-NC)
    Descărcați ca PDF, TXT sau citiți online pe Scribd
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    1K vizualizări34 pagini

    CRD Subsample

    Încărcat de

    Fahmy Abo Walid Kseibi
    Subsampling experiments promote efficient use of space and materials. They are most efficient when there is much variation in response among explants. It reduces the df for experimental error which means a higher F-value is required to detect significant differences among treatments.

    Drepturi de autor:

    Attribution Non-Commercial (BY-NC)
    Descărcați ca PDF, TXT sau citiți online pe Scribd
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    din 34

    Completely Randomized Design with subsampling

    02Smeth1

    Subsampling experiments promote efficient use of space and materials, and measures variability among observational units. They are most efficient when there is much variation in response among explants and little outside variation.

    02Smeth2

    The design is not efficient when there is little variation among explants or when there is a high degree of variability from some other identifiable source. This is because it reduces the df for experimental error which means that a higher F-value is required to detect significant differences among treatments. If there is a little variation among experiments, a CRD without subsampling should be used. If variation from an identifiable source exists, a RCBD with subsampling should be used.
    02Smeth3

    Subsampling
    Often multiple observations are collected on an experimental unit.
    For example, you may have four plants in a single pot each receiving the same treatment. The experimental unit for treatment comparisons being the pot of four plants.

    However, if you are interested in plant height you may measure the height of each of the plants within the pot. You would then have four sampling units per experimental unit.

    02Smeth4

    The main reason for having multiple sampling units per experimental unit is cost. That is the cost for measuring all four plants in each pot is small compared to the cost of measuring a single plant in four times as many pots.

    02Smeth5

    It is very important to note that subsampling does not increase the number of replications. That is in terms of treatment comparisons you might as well analyze the average height of the four plants. The number of replications is still the number of EXPERIMENTAL UNITS per TREATMENT, not the number of observations per treatment.

    02Smeth6

    Replications of treatments are assigned completely at random to independent groups of experimental subjects, such as adjacent trees, plants within the same pot or leaves on the same tree. Adjacent groups could potentially have the same treatment. A group of experimental subjects is considered a single independent experimental unit.

    02Smeth7

    Large EU make subsampling a necessity. In other experiments, the researcher may introduce subsampling in order to study the within variability. Knowledge of this variation may be of value in future research experiments.

    02Smeth8

    Sample layout:
    There are 4 replications (1-4) of 3 treatments (A-C) with 3 subsamples (a-c) per replication. Different colors represent different treatments.
    A1a A1b A1c B2a B2b B2c C3a C3b C3c B4a B4b B4cB1a B1b B1c A2a A2b A2c C2a C2b C2c A4a A4b A4cC1a C1b C1c B3a B3b B3c A3a A3b A3c C4a C4b C4c

    02Smeth9

    Examples on Subsampling
    Example Trt E.U. Sampling Unit

    Agronomy

    Fertilizer Rate

    Plot

    Plot has 6 rows and only harvest 2 rows Row is Sampling Unit

    Animal Science

    Diet

    Pen of 4 Measure 2 calves calves Calf = Sampling Unit Measure 2 samples from beaker, Sample = Sampling Unit
    02Smeth10

    Lab Science Growth media Beaker

    ANOVA with Sampling (Equal Number of Samples Per Experimental Unit)

    02Smeth11

    Model

    02Smeth12

    ANOVA

    02Smeth13

    ANOVA Fixed Effect


    SOV Treat df t-1 SS SST SSE MS MST MSE F-Value MST/MSE

    Exp. Error t(r-1)

    Sampling Error Total

    tr(s-1) SSS
    trs-1 SSY

    MSS

    02Smeth14

    02Smeth15

    Example

    02Smeth16

    Hypothesis

    02Smeth17

    02Smeth18

    02Smeth19

    02Smeth20

    02Smeth21

    02Smeth22

    02Smeth23

    02Smeth24

    ANOVA Fixed Effect


    SOV Treat df t-1 SS SST SSE MS MST MSE F-Value MST/MSE

    Exp. Error t(r-1)

    Sampling Error Total

    tr(s-1) SSS
    trs-1 SSY

    MSS

    02Smeth25

    02Smeth26

    02Smeth27

    Example

    02Smeth28

    Data

    02Smeth29

    ANOVA

    02Smeth30

    02Smeth31

    Trt Plot 1 2 3 4

    1
    2 3 4 5 6 7 8

    02Smeth32

    ANOVA
    Sov Trt Exp. Error df t-1 r.- t

    Sample. Error n.. r. Total n.. - 1

    t = # of treatment. r. = # E.U

    n.. = sample number


    02Smeth33

    ANOVA
    Sov Trt Exp. Error df t1 r.- t = (4-1) = 3 = 26 4 = 22 52 26 = 26 52 1 = 51

    Sample. Error n.. r. Total n.. - 1

    02Smeth34

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