Yeast

Wednesday, March 27, 2013 4:45 PM

30-35C SAB-Dextrose Agar Cornmeal with coverslip => Study structures & Chlamydospores Birdseed/Niger Agar => C. Neoformans = Black/brown colonies ALSO Caffeic Acid Disk Germ Tube = No Constriction; Pseudohyphae = Constriction C. Albicans - Growth at 42C Blastoconidia Pseudohypae Chlamydospores (Thick walled) C. Tropicalis Sparse blastoconidia Long pseudohypae C. Kruseii (Usually non-patho) Oval, elongated blastoconidia at tip of Pseudohyphae. "Matchsticks" Resistant to Fluconazole; Urea +

C. Pseudotropicalis Elongated blastoconidia on Pseudohypae "Logs in a stream"

C. Glabrata Only small, oval blastoconidia

Rhodotorula Sp. (Usually a contaminant) Urea + Pink on SAB agar Round to oval budding yeast

Cryptococcus neoformans Wet Prep: Round, retractile yeast India Ink Capsule; Urea Positive From old bird droppings

Saccharomyces cerevisiae (usually non-patho) Geotrichium Sp. Yeast of various shapes, multilateral budding Produces lesions Few pseudohyphae True hyphae with Arthoconidia Ascospores: Kinyoun Stain (Red = Asci cells)

Trichosporon Sp. True hyphae with pseudohyphae Arthroconidia and blastoconidia "Spaghetti and Meatballs"; Urea +

Malassezia furfur requires fatty acids(olive oil) C. gattii => Less serious than neoformans. Wood's lamp: Red Fluorescence Enviornmental Budding yeast with "colarette" Cryptococcus: Narrow Base Budding "Spaghetti and Meatballs"

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"Spaghetti and Meatballs"; Urea +

"Spaghetti and Meatballs"

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Dimorphics
Wednesday, March 27, 2013 6:28 PM

Yeast at 37C; Mold at RT (30C) Slow Growth Most Pathogenic Group

Histoplasma capsulatum Bird/Bat Droppings Large, Tubercate Macroconidia Microconidia on hyphae

Blastomyces dermatidis Large, Broad-based budding yeast No Macroconidia Smooth-walled conidia

Coccidioides immitis VERY Infectious; Pulmonary Disease Thick arthroconidia w/disjunctor cells in btwn ALSO spherules with endospores

Sperules

Paracoccidioides brasiliensis No true yeast form; "ship's wheel" in tissue Mold: Branched hyphae w/chlamydospores Few microconidia

Sporothrix Schenkii "Rose Gardener's Disease" Cigar-Shaped Budding Yeast Tear-Shaped Conidia

Technecially Also Dimorphic

C. albicans: Grows as mold at 37C (opposite) M. furfur Penecillium

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Hyaline
Wednesday, March 27, 2013 7:16 PM

Opportunistic Very Rapid Growing (3-5 Days) Aspergillus Fumigatus Grows at 45C Uniserate; Conidia only upper 2/3rds Parallel Chains Aspergillus Niger Biseriate; Covers Vesicle Conidial Chains radiate Aspergillus Flavus Carcinogenic (liver cancer) Uni/biserate Covers entire vesicle; points in all directions

Aspergillus Terreus Biserate; Compactly Columnar

Aspergillus nidulans Biserate; short, columnar Ascospores

Aspergillus clavatus

Penecillium

Fusarium

Paecilomyces

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Penecillium Chains of conidia

Fusarium Contact lense/eye infections "Banana Boat" Macroconidia; Microconidia Pink/Lavender Colony

Paecilomyces Resembles Penecillium; phialides elongated

Acremonium Septate Hyphae; oblong conidia Tapering phialides, unbranches "Candle with a flame"

Almost all others are contaminants Except Scopulariopsis (an annelide): Common nail infection

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