UNIVERSITI TEKNOLOGI MARA FACULTY OF CHEMICAL ENGINEERING PROCESS & THERMODYNAMIC LAB (CPE 554)

NAME STUDENT ID EXPERIMENT DATE PERFORMED PROG/CODE

: ZANARIAH BINTI ISMAIL : 2010228408 : Distillation Collumn : 19th October 2012 : Bachelor of Engineering (Hons.) in Chemical & Bioprocess Engineering/EH222/5B Allocated Marks % 5 5 5 5 5 5 10 10 20 10 10 5 5 100 Rechecked by: Marks %

NO 1 2 3 4 5 6 7 8 9 10 11 12 13

Title Summary Introduction Objectives Theory Procedures Apparatus Results Calculation Discussion Conclusion Recommendation References Appendices TOTAL Miss Siti Norazian bt Ismail

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Laboratory 1: Growth Kinetics Study of Microorganism in Shake Flask 1. AThis experiment is carried out to study the growth kinetics of microorganisms in shake flask. E. coli is grown in a LB broth medium and being fermented for 24 hours. Throughout the fermentation, the cell culture is taken out for every 3 hours and protein test, glucose test and cell dry weight are being performed. As for the optical density analysis, the absorbance reading from the spectrophotometer is taken while for the glucose test, the reading of glucose level is taken from the YSI 2700 Select Biochemical Analyzer or can also being performed by using DNS reagent and the absorbance value is taken. These absorbance values will then being compared with the standard curve to get the glucose concentration inside the shake flask at particular time. The cell dry weight, in the other hand, is taken after the mass concentration is being dried overnight in the oven. The weight of the viral which contains the biomass before and after the drying process is recorded to get the dry cell weight. For the optical density of the cell, the absorbance value showed an increment which indicating that the cell was growing and number of cell is increased in the shake flask. The glucose concentration, however, cannot be determined as the absorbance values were increased and decreased unevenly and comparison cannot be made with the standard curve as the data for the standard curve are not consistent giving inaccurate curve. Therefore no conclusion can be made about the glucose concentration in the shake flask. Supposedly, as the number of cell increased, the glucose concentration would decrease as the glucose consumption by the cells is increased. The dry cell weight in the other hand can be seen that there is an increment from the beginning of the cultivation until the 6thhour and showed unstable changes until the 24 th hour. Supposedly, as the number of cell increased inside the shake flask, the cell dry weight also should be increased

Introduction