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These customized fibres will be tailored to the need of the textile industry. New properties may includegreater fibre strength, enhanced dyeability, improved dimensional stability, reduced tendency for shrinking and wrinkling and altered absorbency. Greater strength will allow higher spinning speeds and improved strength after wrinkle-free treatments. Improved reactivity will allow more efficient use of dyes. Thusreducing the amount of colour in effluents. To reduce the waste generated during scouring and bleaching processes, it would be interesting to have fibres with less of pectins, waxy materials and containing enzymes that can biodegrade environmental contaminants. These fibres would be placed in filters through which contaminated water is passed.
NOVEL FIBRES:
The use of biotechnology has the potential of control and specificity in polymer synthesis which is difficult, if not impossible, to achieve in chemical systems. New materials produced using advanced biologically based approaches represent the textiles of the future.
Protein Polymers: Biological systems are able to synthesize protein chains in which molecular weight, stereochemistry, amino acid composition and sequence are genetically determined at the DNA level. A current area of investigation is to understand those features of protein polymers that confer high tensile strength, high modulus and other advantageous properties. Once those features are understood, the tools of biotechnology will make possible entirely new paradigms for the synthesis and production of engineered protein polymers. If they can be made economically viable, these new approaches will help to reduce the dependence on petroleum and furthermore will enable the production of materials that are biodegradable. Use of transgenic plants for arge-scale production of these and other synthetic proteins is being explored.
Efforts in biosynthesis have been directed towards the preparation of precisely defined polymers of three kinds
(1) Natural proteins such as silks, elastins, collagens and marine bioadhesives, (2) Modified versions of these biopolymers, such as simplified repetitive sequence of the native protein, and (3) synthetic proteins designed de novo that have no close natural analogues. Although such syntheses pose significant technical problems, these difficulties have
all been successfully overcome in recent years. Using this technology, a whole new class of synthetic proteins with advanced properties, known as bioengineered materials, is being created.
Spider silk: Spider dragline silk is a versatile engineering material that performs several demanding functions. The mechanical properties of dragline silk exceed those of many synthetic fibres. Dragline silk is at least five times as strong as steel, twice as elastic as nylon, waterproof and stretchable. Moreover, it exhibits the unusual behavior that the strain required to cause failure actually increases with increasing deformation. Other New Fibres Sources: There are many more biopolymers, of particular interest in sanitary and wound healing applications, which include bacterial cellulose and the polysaccharides such as chitin, alginate, dextran and hyaluronic acid.Some of these are discussed below: Chitins and Chitosans: Chitins and chitosans both can form strong fibres. Chitin is found in the shells of crustaceans, such as crab, lobster, shrimps etc. Resembling cellulose, the chitin consists of long linear polymeric molecules of beta- (1-4) linked glycans. The carbon atom at position 2, however, is aminated and acetylated. Fabrics woven from them are antimicrobial and serve as wound dressing products and as anti-fungal stockings. Chitosan also has promising applications in the field of fabric finishing, including dyeing and shrink proofing of wool. It is also useful in filtering and recovering heavy and precious metals and dyestuffs from the waste streams. Wound dressing based on calcium alginate fibres are marketed by Courtaulds under the trade name Sorbsan. Present supplies of this polysaccharide rely on its extraction from certain species of bacteria. Dextran, which is manufactured by the fermentation of sucrose by Leuconostoc mesenteroides or related species of bacteria, is also being developed as a fibrous nonwoven for specialty end uses such as wound dressings. Additional biopolymers, not previously available on a large scale, are now coming into the market, thanks to biotechnology.
Schematic diagrams of the model describing the process of lead adsorption on chitin bead
(a) Formation of complexation, (b) adsorption of addition lead, (c) micro precipitations
Bacterial cellulose: Cellulose produced for industrial purposes is usually obtained from plants sources or it can be produced by bacterial action. Acetobacter xylinium is one of the most important bacteria for cellulose production as sufficient amounts can be produced which makes it industrially viable. Cellulose produced by Acetobacter, which has the ability to synthesize cellulose from a wide variety of substrates, is chemically pure and free of lignin and hemicellulose. Cellulose is produced as an extra cellular polysaccharide in the form of ribbon like polymerization, high tensile strength and tear resistance and high hydrophilicity that distinguishes it from other forms of cellulose. This bacterial cellulose is being used by Sony Corporation of Japan in
acoustic diaphragms for audio speakers. They are also being used in the production of activated carbon fibre sheets or absorption of toxic gas and as thickeners for niche cosmetic applications. In medical field, because of the hydrophilic and mechanical properties of bacterial cellulose, it is used temporarily as skin substitute and in wound healing bandages.
Corn fibre: An entirely new type of synthetic fibre derived from a plant is Lactron. This environment friendly corn fibre was jointly developed by Kanebo Spinning and Kanebo Gohsen of Japan. Lactron, the polylactic acid fibre is produced from the lactic acid obtained through the fermentation of corn starch. Strength stretchability and other properties of Lactron are comparable to those of petrochemical fibres such as nylon and polyester. As mthe material is compatible with human body, it is being used for sanitary and household applications. In addition to clothing the company is also promoting its non-clothing applications, e.g. construction,agricultural, papermaking, auto seat covers and household use. The energy required for production of corn fibre is low and the fibre is biodegradable. Moreover, no hazardous gases are created when it is incinerated and the required calories for combustion are only one-third or half of those required by polyethylene orpolypropylene. It safely decomposes into carbon dioxide, hydrogen and oxygen when disposed of in soil.Lactron is being marketed in various forms such as woven cloth, thread and non-woven cloth.
Polyester fibres: It has been known since 1926 that certain polyesters are synthesized and intracellulose deposited in granules by many micro-organism. Some of these materials have been formed into fibres.Polyhydroxybutyrate (PHB) is an energy storage material produced by a variety of bacteria in response to environmental stress. It is being commercially produced from Alcaligenes eutrophus by Zeneca Bioproduct sand sold under the trade name Biopol. As PHB is biodegradable, there is considerable interest in using it for packaging purposes to reduce the environmental impact of human garbage. Thus it is already finding commercial application in specialty packaging uses. Because of its immunological compatibility with human tissue, PHB also has utility in antibiotics, drugs delivery, medical suture and bone replacement applications.
BIOFABRICS:
The development of biocidal fabrics was based on the idea of activating textiles with reactive chemicals to impart desirable properties. The latest research however is aimed at producing fabrics containing genetically engineered bacteria and cell strains to manufacture the chemicals within the textiles thereby making the chemical stores within the fabrics the self-replenishing materials. A collaborative project is on between the textile science research team at University of Massachusetts, Dartmouth and the bio-engineers at Harvard medical to carry out research leading to the production of a class of fabrics with special properties called biofabrics. Biofabrics will contain micro-fabricated bio-environments and biologically activated fibres. These fabrics will have genetically engineered bacteria and cells incorporated into them that will enable them to generate and replenish chemical coatings and chemically active components.Niche applications for bio-active fabrics exist in the medical and defense industries, e.g. drug producing bandages or protective clothing with highly sensitive cellular sensors, but biofabrics may form the basis of a whole new line of commercial products as well e.g. fabrics that literally eat odours with
genetically engineered bacteria, self cleaning fabrics, and fabrics that continually regenerate water and dust repellents. For such an approach to be successful, technologies will have to be developed to micro-fabricate devices able to sustain cellular or bacterial life for extended periods, exhibit tolerance to extremes of temperature, humidity and exposure to washing agents, as well as tolerance to physical stress on the fabrics such as tension, crumpling and pressure2.
The various industrial applications of biotechnology have a number of things in common, both in terms of improved output and reduced environmental footprint. They can deliver some or all of the following benefits: Reduce water use and traditional chemicals Reduce use of energy, and thus lower levels of CO2 emissions. Conversion of many existing chemical processes will make a significant contribution towards meeting the targets set by the Kyoto treaty. Increase the use of renewable resources, whether as chemical feed stocks or fuels. Growing rather than extracting will reduce the use of fossil fuels and be carbon-neutral. Biotechnological processes, because they are precisely targeted, can be used to make new materials and higher quality materials more cost effectively, with less waste. Cell cultures are unique in their capacity to make new pharmaceuticals and vaccines which could not otherwise be made Bio-based industries can also give a major boost to European agriculture by for example sourcing high-value raw materials from farmers, providing new alternatives for agricultural and use and using agricultural waste to build value: a clear contribution towards a sustainable rural economy. White biotechnology is highly selective. For example, Vitamin B2 can be made using a one step bioprocess rather than by a chemical synthesis that makes mixtures which then have to be separated using precious water and energy resources. In doing so waste by-products are produced which then need to be treated. This is why introducing bioprocesses into production can help companies produce cheaper, cleaner and often superior products.
Waste Management
Biotechnology can be used in new production processes that are themselves less polluting than the traditional processes and microbes or their enzymes are already being used to degrade toxic wastes. Waste treatment is probably the biggest industrial application of biotechnology. Specific problems pertaining to the textile industry include colour removal from dye house effluent, toxic heavy metal compounds and pentachlorophenol used overseas as a rotproofing treatment of cotton fabrics but washed out during subsequent processing in the developed countries.
Detergents
An enzyme allows detergents to effectively clean clothes and remove stains. They can remove certain stains, such as those made by grass and sweat, more effectively than enzymefree detergents. Without enzymes, a lot of energy would be required to create the high temperatures and vigorous shaking needed to clean clothes effectively. Enzymes used in laundry detergents must be inexpensive, stable, and safe to use. Currently, only protease and amylase enzymes are incorporated into detergents. Lipase enzymes break down too easily in washing machines to be very useful in detergents. However, their stability is being studied and further developed through methods such as genetic screening and modification.
Amylase, pectinase, and glucose oxidase are enzymes used for desizing, scouring, and bleaching respectively in enzymatic preparation processes. Desized samples show completely size removal using amylase enzyme. Samples scoured with pectinase are immediately and uniformly wet. Amount of pectin and other substances left on scoured samples from both conventional and enzymatic processes were measured along with sample strength and whiteness index. Samples bleached with glucose oxidase obtain whiteness ndex 15-20 degree improvement with low strength loss. Conventional preparation of cotton requires high amounts of alkaline chemicals and consequently, huge quantities of rinse water are generated. An alternative to this process is to use a combination of suitable enzyme systems. Amyloglucosidases, Pectinases, and glucose oxidases have been selected that are compatible concerning their active pH and temperature range. A process has been developed that allows the combination of two or all three preparation steps with minimal amounts of treatment baths and rinse water. Whiteness, absorbency, dyeability and tensile properties of the treated fabrics have been evaluated .
The use of biocatalyst in the textile industry is already state of the art in the cotton sector. Research and development in this sector is primarily concentrating on: Optimizing and making routine the use of technical enzymes in processes that are already established in the textile industry today. Preparing enzyme-compatible dyestuff formulations, textile auxiliary agents and chemical mixtures.Producing new or improved textile product properties by enzymatic treatment. Providing biotechnological dyes and textile auxiliary agents, which are suitable for industrial use, and can possibly be synthesized in-situ (i.e. on-line for the application process).
Extremophile Micro-Organisms: Numerous micro-organisms have learnt to live in very different and difficult environmental conditions, e.g.in high temperatures, in acid and alkaline conditions and in the presence of salt concentrations. These extremophile micro-organisms live in the most inhospitable and unspoilt environments on earth. Where other microorganisms do not exist, they are to be found in the deepest oceans under pressures of more than 100 bar, in hot volcanic sources at over 100 C in cold regions at temperatures around freezing point, in salt lakes (up to 30% salt concentration) and also in surroundings with extreme pH values (pH <2,> 9).The cell components (enzymes, membranes) of extremophile are optimally adapted to extreme environmental conditions, and have characteristics (stability, specificity and activity), which make them interesting for biotechnological application. At the Hamburg-Harburg (D) University of Technology, a comprehensive screening programmed for isolating exremophile micro-organisms (like starch, proteins, and hemicellulose for example) has been implemented which is able to produce enzymes for breaking down biopolymers, alkanes, polyaromatic carbohydrates (PAK) plus fats and oils. Within the framework of these studies, a range of biotechnologically relevant enzymes like amylases, xylanases, proteases, lipases and DNA polymerases for example have been enriched and characterized. Conversion of Natural Polymers By Extremozymes: Starch is one of the most important biopolymers on this earth. The macromolecule built up from glucose units, plays an outstanding role in the food industry under the collective concept modified starch this is found in many foods. Amylases and branching enzymes for example are used for modifying starch. With the aid of thermostable starch-modifying enzymes, starch finishing can be carried out more purposefully and efficiently, since for example the space-time yield at high temperatures is significantly better due to improved starch solubility. Thermo-alkalistable enzymes (active at pH >8 and 600C) are used in washing and harness rinsing agents in order to remove tenacious starch accumulations with simultaneous reduction n detergent quantity. Cyclising Enzymes: So-called cylodextrins can be produced from starch with the aid of cyclising enzymes cyclodextringlycosyl- transferase, CGTase) from the recently isolated thermoalkaliphile bacterium Anaerobranca gottschalkii. Hydrophobic active substances or volatile aromas can be encapsulated in these cyclodextrins. Cyclodextrins were isolated by Villiers as early as 1891. In those days, cyclodextrins were regarded as curiosities of no technical value.The properties of cyclodextrins have been altered by chemical change (derivatives). The target of much research work is to fix a reactive cyclodextrin derivative on cellulosic or protein fibres by forming a new chemical bond on the fibre. The molecules have a hollow space, which is suitable for absorbing diverse substances like perfume for example. Many application possibilities and effects arise out of this complexing like for example: Increased water solubility Change of rheological characteristics Stabilization against UV radiation, thermal disintegration, oxidation and hydrolysis Reduction of unpleasant smells Absorption of microbe-eliminating products
Cellulose from Extremophile Micro-Organisms: Cellulose is also a biopolymer built up of glucose units. It forms the framework of higher plants and is an important resource in the textile industry. The use of cellulases in detergents leads to colour revival (colour detergent) and the improved removal of vegetable soiling. Cellulases are also successfully used in biostoning. In contrast to conventional cellulases, which are obtained from mesophilic fungi as a rule,cellulose-hydrolyzing enzymes from extremophile micro-organisms have the advantage of being capable of use even at high temperatures and pH values. Xylanolytic enzymes: Xylanolytic enzymes form another group. Xylan is heterogeneous molecule (basic component: xylose sugar), which makes up the largest proportion of the polymeric vegetable cell wall component hemicellulose. Xylanophile micro-organisms have enormous biotechnological potential. Thermobile xylanases are already being produced on an industrial scale today, and are used as fodder and food additives. In past years, interest in xylanases was concentrated particularly on enzymatic paper bleaching. Current studies have shown that the enzymatic treatment of paper is an ecologically and economically sound alternative to the hitherto employed chlorinebased bleaching process. Enzymes which can for example destroy the coloured attendant substances of cotton are of interest to the textiles industry. The quantity of caustic soda and salt required in peroxide bleaching could be reduced by this type of enzymatic bleaching. Reuse of the bleaching liquor after hydrogen peroxide bleaching is already possible today by using the enzyme catalase after bleaching. This enzyme destroys excess hydrogen peroxide, making use of the bleaching liquor for other finishing stages possible. Windel Textil GmbH & Co. (D) already uses the so-called Bleach-Cleanup process, in which bleaching agent residues are removed from textiles, resulting in a reduction of energy, time and water-intensive washing operations at high temperatures.Research projects at the German Wool Research Institute (DWI) in Aachen (D) are devoted to the use of enzymes in wool processing, including the removal of vegetable residues from the wool, increasing the degree of whiteness, improving handle, improving dyeability by increasing intensity of colour and for feltfree finishing. Already interesting in practice is the felt-free finishing of wool. An enzyme not previously employed in the textile industry modifies the scale-like surface of wool fibres preventing felting. The enzyme Lanazym has hitherto been used only in discontinuous batch processing.3 DECOLOURISATION OF DYES BY USING BIOTECHNOLOGY: The synthetic dyes are designed in such a way that they become resistant to microbial degradation under the aerobic conditions. Also the water solubility and the high molecular weight inhibit the permeation through biological cell membranes. Anaerobic processes convert the organic contaminants principally into methane and carbon dioxide, usually occupy less space, treat wastes containing up to 30 000 mg/l of COD,have lower running costs and produce less sludge4. Azo dyes are susceptible to anaerobic biodegradation but reduction of azo compounds can result in odour problems. Biological systems, such as biofilters and bioscrubbers, are now available for the removal of odour and other volatile compounds. The dyes can be removed by biosorption on apple pomace and wheat straw5. The experimental results showed that 1 gm of apple pomace and 1 gm of wheat straw, with a particle size of 600m, were suitable adsorbents for the removal of dyes from effluents. Apple pomace had a greater capacity to adsorb the reactive dyes taken for the study compared to wheat straw. Decolourization of the Dye House Effluent Using Enzymes: The use of lignin degrading white-rot fungi has attracted increasing scientific attention as these organisms are able to degrade a wide range of recalcitrant organic compounds such as polycyclic aromatic hydrocarbons, chlorophenol, and various azo, heterocyclic and polymeric dyes. The major enzymes associated with the lignin degradation are laccase, lignin peroxidase, and manganese peroxidase. The
laccases are the multicopper enzymes, which catalyze the oxidation of phenolic and non-phenolic compounds. However, the substrate of the laccases can be extended by using mediators such as 2, 2-azoinobis-(3-ethylthiazoline-6-sulfonate), 1-hydroxybenzotriazole. The following fungi have been used for laccase production and for the decolourization of synthetic dyes. Trametes Modesta, Trametes Versicolour, Trametes Hirsuta, and Sclerotium Rolfsii 6 from the results obtained it was clear that Trametes Modesta laccase showed the highest potential to transform the textile dyes into colourless products. The rate of the laccase catalyzed decolourization of the dyes increases with the increase in temperature up to certain degree above which the dye decolourization decreases or does not take place at all. The optimum pH for laccase catalyzed decolourization depends on the type of the dye used. Dyes with different structures were decolourized at different rates. From these results it can be concluded that the structure of the dye as well as the enzymes play major role in the decolourization of dyes and it is evident that the laccase of Trametes Modesta, may be used for decolourization of textile dyestuffs, effluent treatments, and bioremediation or as a bleaching agent. Another study carried out by E. Abadulla et al, has shown that the enzymes Pleurotus ostreatus, Schizophyllum Commune, Sclerodium Rolfsii, Trametes Villosa, and Myceliophtora Thermiphilia efficiently decolourized a variety of structurally different dyes. This study also shows that the rate of reaction depends on the structure of the dye and the enzyme7. Activated sludge systems can also be used to treat the dyehouse effluents. But the main difficulty with activated sludge systems is the lack of true contact time between the bacteria of the system and the suspended and dissolved waste present. Immobilized microbe bioreactors (IMBRs) address the need of increased microbial/waste contact, without concomitant production of excessive biosolids, through the use of a solid but porous matrix to which a tailored microbial consortium of organisms has been attached. This allowed greater number of organisms to be available for waste degradation without the need of a suspended population and greater increased contact between the organisms and the waste in question8. CONCLUSION: The advent of biotechnological applications in textile processing widens the already existing wide horizons to produce aesthetically colourful magnanimous and ecologically friendly textiles, ringing in a new era of synergetic application of life sciences. As of today the huge textile industry is open to welcome the immense possibility of the various biotechnological applications limited by the limitations of being eco friendly and not harming either the food web or the life cycle of any other living creature. Such awarenessis gradually metamorphosing a tool that could be intelligently used to meet the demand of our fashion trends. Enzymes, bacteria and insects could be biologically modified into a fashion promoter if engineered with great caution. A major breakthrough in the textile industry is eagerly awaited through these biotechnological applications.
REFERENCES: Biotechnology, Edited by H. J. Rehm and G. Reed Biotechnology application in textiles industry, Deepti Gupta, Indian Journal of Fibres & Textile Research Vol.26, March-June 2001.Biotechnology: process and products, Andrea Bohringer, Jurg Rupp, International Textile Bulletin, June 2002. The Biotechnology Approach to Colour Removal from Textile Effluent, by Nicola Willmott et al. J of Soc. Of Dyers and Col., 1998, 114, 38-41. Removal of dyes from a synthetic textile dye effluent by Biosorption, by T. Robinson, et al, Water Research, 36, 2002, 2824-2830. Decolourization of textile dyes by laccases, by G. S. Nyanhongo, et al, Water Research, 36, 2002, 1449-1456. Enzymatic decolourization of Textile Dyeing Effluents, by E. Abadulla et al, Textile Res. J. 70