GATE HELPLINE

BIOPROCESS ENGINEERING MCQs-III

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21. Measurement of KLa in a bioreactor can be carried out by sodium sulfite oxidation method that is based on the oxidation of sodium sulfite solution containing 0.003 M Cu++ ions and the air was sparged in. After 10 minute the air flow was stopped and a 10 ml sample was taken and titrated. The concentration of the sodium sulfate in the sample was found to be 0.20 M. the oxygen uptake rate for this aerated system will work out to be: a) 0.08 g l-1 s-1 c) 0.8 g l-1 s-1 -1 -1 b) 0.008 g l s d) none of the above 22. Examine the date given below in the table on purification of a protein X Step Volume Protein Crude cell free extract 500 12.0 Ammonium sulfate precipitation 125 3.0 Ion exchange chromatography 10 9.0

Enzyme activity 5.0 11.0 75.0

The yield percent and purification factor respectively at the end of the experiment will be approximately a) 25 and 3 c) 20 and 30 b) 75 and 30 d) 30 and 20 23. Which one of the following reactions is used for the purpose of recycling enzymes in bioprocesses? a) Isomerization c) Phosphorylation b) Polymerization d) Immobilization 24. To optimize the bioreactor system, which one of the following conditions is least important for anaerobic fermentation? a) Culture agitation to maintain oxygen supply b) Restriction of the entry of contaminating organisms c) Control of parameters like pH and temperature d) Maintenance of constant culture volume 25. a) b) c) d) Power number, also Newtons number. Is defined as a dimensionless parameter relating to Turbulent flow The relative velocity between the nutrient solution and individual cells Energy required by the stirred reactors None of the above

26. The selection of the appropriate purification method in the product recovery after microbial fermentation depends on the a) Nature and the stability of the end products produced b) Type of the side products present c) Degree of purification required d) All of the above 27. a) b) c) d) Which of the following techniques is NOT ideal for immobilizing cell- free enzymes? Physical entrapment by encapsulation Covalent chemical bonding to surface carriers Physical bonding by flocculation Covalent chemical bonding by cross linking the precipitate This is copyrighted product of GATEHELPLINE

28. If the fractional recovery at each step of unit operation is 0.8, the recovery after 4 step will be a) 0.24 c) 0.41 b) 3.23 d) 0.82 29. The factors likely to increase the rate of reaction catalyzed by a surface immobilized enzyme is/are a) Increased agitation of the bulk liquid containing the substrate b) Continued replacement of the bulk liquid containing the substrate c) Increased concentration of the substrate in the bulk liquid d) All of the above 30. Which of the following cases are likely to lead to faster rates of catalysis by an enzyme immobilized on a negatively charged support? a) A positively charged substrate and a negatively charged product b) A negatively charged substrate and a positively charged product c) A positively charged substrate and a positively charged product d) None of the above 31. a) b) c) d) Which of the following statements applies to the operation of a fed batch process? The volume of the culture increases with time It helps controlling repressive effects of the nutrient being fed It eliminates the need for oxygen supply Only (A) and (B) of the above

32. In large scale fermentation, the preferred method of sterilization is a) Chemicals c) radiation b) Filtration d) heat 33. a) b) c) d) 34. a) b) c) d) 35. a) b) c) d) In secondary metabolism two distinct trophophase and idiophase refer respectively to Growth and production phase Early and late phase Primary and secondary metabolism Lag phase and log phase Which of the following is true for chemostat? Concentration of limiting substrate is maintained constant High productivity can not be achieved Automation is difficult Mutation is less likely to occur For scaling up of a bioreactor, the following parameter is assumed to be constant Airflow rate Diameter of the impeller Agitator speed Volumetric mass transfer coefficient

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36. a) b) c) d)

Mechanism of separation of contaminants present in air by fibrous media are Interception Inertial impaction Diffusion All the above

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