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woensdag 15 mei 13
Lameness in horses
Orthopedic problems with lameness is the most common problem in equine veterinary practice.
The lameness examination aims to:
rst localize the origin of pain, then obtain more information about that source of pain, and nally to determine the best possible treatment.
Lameness in horses
Diagnostic methods in investigation of lameness in horses
Trotting-up and joint exion tests Nerve blocks or joint blocks Imaging techniques (radiographs, ultrasound, scintigraphy, MRI) Synovial uid (joint uid) analysis
Cell count
Hemocytometer + microscope
There is need for an automated nucleated cell counting method for synovial uid that would be faster, easier, and more precise.
Boule 2013-02-07 woensdag 15 mei 13
Article
Test for the optimal use of hyaluronidase enzyme on viscous equine synovial uid samples
Equine synovial samples with (normal) low cell counts (WBC <1x109/L, n=3), in EDTA-tubes These samples gave instrument error ags due to (normal) viscosity Treatment with Hyaluronidase (Roche Diagnostics Scand. AB, Sweden) was tested to abolish viscosity-related instrument error Tested nal concentrations of enzyme in synovial uid: 1 to 0.000025 mg/ml diluted in the hematology analyzers diluent (Mediton II-Vet, Boule Medical AB, Sweden)
This PowerPoint presentation was prepared by Gittan Grndahl, DVM, PhD National Veterinary Institute Uppsala, Sweden
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