Herbal medicine , also called botanical medicine or phytomedicine,refers to the use of any plant seeds, berries, roots, leaves,

bark or flowers for medicinal purposes. There are many proceses patented for commercial extraction of plant ingredients. The general techniques of extraction of medicinal plants include maceration,percolation & hot continous extraction (soxhlet). Maceration is the simple method by soaking the powder material in the solvent for breaking the cell wall and foll.owed by destilation proces 1. Extraction Ethanol Absolute
Fresh Grass Mincing

Shadow dried/ oven 60 C

Grinding (Powder)

1kg of powdered plant Soaked in 10 L water 600C, 20 min

Filter to obtain crude yellow aquaeous extract

Add 100% Ethanol absolute 1:4

Filter the supernatant solution and gel pellet was discharge

yellow extract destilated 600C to get concentrated extract

2. Extraction Ethanol 96% (technical grade)

Fresh Grass Mincing

Shadow dried/ oven 60 C

Grinding (Powder)

1kg of powdered plant Soaked in 10 L water 600C, 20 min

Filter to obtain crude yellow aquaeous extract

Add 96% Ethanol technical grade 1:4

Filter the supernatant solution and gel pellet was discharge

yellow extract destilated 600C to get concentrated extract

3. Extraction Water 8-10 times

Fresh Grass Mincing

Shadow dried/ oven 60 C

Grinding (Powder)

1kg of powdered plant Soaked in 10 L water 600C, 20 min

Filter to obtain crude yellow aquaeous extract

8-10X
Add powdered plant 1/ 10 (w/v) from yellow extract

Filter the crude yellow aquaeous extract

Coated to the feed

4. Methanol extract
Fresh Grass Mincing

Shadow dried/ oven 60 C

Grinding (Powder)

550g of powdered plant Soaked in 3 L methanol for 7 day

Filtered through cotton plug Followed by whatman filter paper no 1

Extract destilated 600C To get concentrated extract

5. Directly mixing to the feed

Fresh Grass Mincing

Shadow dried/ oven 60 C

Grinding (Powder)

Mix to the feed 15 -20 %

EXTRACT PARAMETER Menurut buku Monografi Ekstrak dan Parameter Standar Ekstrak (Badan POM), parameter yang harus diujikan terhadap ekstrak sedikitnya antara lain meliputi :

A. Parameter spesifik 1.Identitas ekstrak ( extract identity) 2.Organoleptik ekstrak (organolepthic extract) 3.Senyawa terlarut dalam pelarut tertentu (soluble compound & solvent) B. Parameter non spesifik 1.Susut pengeringan 2.Bobot jenis (specific gravity) 3.Kadar air (moisture) 4.Kadar abu (ash) 5.Sisa pelarut (solvent residue) 6.Residu pestisida (pesticide residue) 7.Cemaran logam berat (heavy metal) 8. Cemaran Mikroba : (microbacterial contamination) a.ALTB b.MPN Coliform c.Uji Angka kapang dan khamir (mold) d.Uji cemaran aflatoksin (aflatoxin) C. Uji kandungan kimia ekstrak 1.Pola kromatogram (chromaogaphi characteristic) 2.Kadar total golongan kandungan kimia (Total chemical content) 3.Kadar kandungan kimia tertentu (specific chemical content)

MACHINE FOR GRXTRACTION

Grass Cutter

OVEN

Hammer Mill

Mixer + heater

Filter Press

Rotary Evaporator 50 l/h

Alcohol/Ethanol destilator