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Liquid Proprietary formulation, modified version of MCDB 105 Contains essential and non-essential amino acids, vitamins, trace minerals, organic and inorganic salts. Without antibiotics, antimycotics, hormones, growth factors, or proteins. Buffered with HEPES and sodium bicarbonate Sterile filtered Endotoxin tested Cell culture tested.
This basal medium is a component of our Fibroblast Proliferation Medium Kit (FMK-2). It is designed to be used in conjunction with Fibroblast Medium Supplement (F 9915), and it is not sold separately. The FMK-2 kit provides a complete medium for the proliferation of epidermal fibroblasts for research purposes.
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Liquid Proprietary formulation Contains fetal bovine serum (2%), human recombinant bFGF heparin, human recombinant EGF, and hydrocortisone
This supplement is a component of our Fibroblast Proliferation Medium Kit (FMK2). It is designed to be used in conjunction with Fibroblast Basal Medium (E 9790), and it is not sold separately. The FMK-2 kit provides a complete
T 4299 Trypsin-EDTA Solution for Endothelial Cell Culture
Endotoxin tested Cell culture tested Liquid Contains 500 BAEE units porcine trypsin and 180 ug of EDTA74Na per mL in DPBS without calcium and magnesium Powder negative for porcine parvovirus by 9 CFR Sterile filtered Mycoplasma tested Cell culture tested Liquid Phosphate buffered saline solution containing (1x) 0.025% soybean trypsin inhibitor pH approximately 7.4 at room temperature Sterile filtered
Useful for the dissociation of, or release of, adherent cells types, such as endothelial, epithelial, and fibroblast cells from support surfaces.
T 7659 Trypsin Inhibitor Defined
Use to inhibit the proteolytic action of Trypsin-EDTA (T 4299) after epithelial cells are released from a substrate or disaggregated. Chemically defined and free of animal derived components. The absence of animal derived components reduces the potential for contamination of cryopreserved cells with adventitious biological agents such as viruses.
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Sigma is proud to offer a selection of media, many of which are serum-free, for the in vitro culture of human primary cells, such as keratinocytes, corneal epithelial cells, endothelial cells, fibroblasts, melanocytes and epithelial hepatocytes. These primary cell types have potential commercial value in a wide range of emerging technologies and therapeutic applications. Among these are: tissue engineering applications, such as would healing and burn treatment; high throughput screening for drug toxicology and efficacy studies; gene therapy; pseudo-organs (e.g.. artificial liver) and as model cell systems for developing new therapeutic approaches to human diseases.