Parasitology - Amoeba

Student Lab Study Questions Multiple Answer (circle all of the correct responses): 1. Identify the criteria used to identify the intestinal protozoa Amoeba. a. Unicellular organism b. Multi-celled organism c. Trophozoite form has locomotion by extension of pseudopodia d. Trophozoite form has locomotion by flagella e. Cyst form is spherical f. Cyst form is amoeboid-shaped g. Cyst form has only one nucleus h. Cyst form has 1, 2, 4, or 8 nuclei 2. Identify the morphologic differences between cysts and trophozoites. a. Cysts have 1, 2, 4, or 8 nuclei b. Trophozoites have up to 8 nuclei c. Cysts are spherical d. Trophozoites are spherical e. Trophozoites are motile f. Cysts are motile Multiple Choice (select the one best answer): 3. What key morphologic criteria is/are used to definitively identify Entamoeba histolytica trophozoites? a. Unidirectional motility b. Karyosome is eccentric in nucleus c. Ingested RBCs d. Both A and C e. Both B and C 4. What genus has a nucleus that has a single karyosome located centrally or an eccentrically placed karyosome surrounded by a nuclear membrane on which is deposited a distinct ring of peripheral chromatin? a. Endolimax b. Entamoeba c. Iodamoeba d. Blastocystis 5. How is Entamoeba hartmanni differentiated from Entamoeba histolytica? a. Size b. Appearance of nucleus c. Presence/absence of ingested RBCs d. Both A and C e. Both B and C 6. The most prominent diagnostic feature seen in the cyst of Iodamoeba butschlii is a/n a. Peripheral chromatin b. Centrally located karyosome c. Ingested RBC d. Glycogen vacuole

Amoeba CLS 419 Student Laboratory

Study Questions

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7. What parasite is the only intestinal amoeba that is pathogenic and causes amoebic dysentery? a. Entamoeba hartmanni b. Entamoeba histolytica c. Entamoeba coli d. Endolimax nana Matching: (single answer may be used more than once) 8. Match the free-living amoeba with its description. _____ Causes corneal ulcerations that can lead to blindness _____ Causes meningoencephalitis, which is usually fatal _____ Life cycle stages include: trophozoite, cyst and flagellate _____ Cyst stage has double-layered, polygonal walls that give clusters of cysts the appearance of a honeycomb Matching: Match the picture of the amoeba with its identification (please pay close attention to key differentiating characteristics): 9. Identify these trophozoites taking note of differing characteristics. _____ Entamoeba histolytica _____ Entamoeba coli _____ Entamoeba hartmanni _____ Iodamoeba butschlii _____ Endolimax nana 45 m
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a. Acanthamoeba b. Naegleria

a.

8 m

b.

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c.

18 m

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15 m 5 m

50 m

d.

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e.

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f.
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Amoeba CLS 419 Student Laboratory

Study Questions

10. Identify these cysts taking note of differing characteristics. _____ Entamoeba histolytica _____ Entamoeba coli _____ Entamoeba hartmanni _____ Iodamoeba butschlii _____ Endolimax nana _____ Blastocystis hominis
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7 m

15 m

a.

b.
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18 m
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21 m

c.
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d.
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e.

35 m

5 m

f.

Amoeba CLS 419 Student Laboratory

Study Questions

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