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Material Safety Data Sheet

3% Acetic Acid with Methylene Blue 1 Product and Company Identification


1.1 Product Name: 1.2 Catalog Number: 1.3 Synonyms: 1.4 Product Use: 1.5 Manufacturer/Supplier: 3% Acetic Acid with Methylene Blue 07060 Not Available Cell Culture StemCell Technologies Inc. th Suite 400, 570 West 7 Avenue Vancouver, BC V5Z 1B3 Canada

Cat. No: 07060

1.6 In Case of Emergency Call: 604-877-0713 1.7 Date Effective: 1.8 Prepared By: December 4, 2012 Quality Control

Composition / Information on Ingredients


2.1 Component Acetic Acid CAS No. 64-19-7 %W/W 3

Hazards Identification
3.1 Emergency Overview: 3.2 Routes of Exposure: 3.3 Potential Health Effects: 3.3.1 Eye: 3.3.2 Skin: 3.3.3 Inhalation: 3.3.4 Ingestion: May cause eye irritation, redness, pain and tearing. May cause skin irritation, redness and pain. Not likely to be hazardous by inhalation. May cause irritation of the digestive tract. Not Available. Not Available. Does not present any significant health hazards. Wash areas of contact with water. If ingested, dilute with water and call a physician. Absorbed through skin, eye contact, inhalation, and ingestion.

3.4 Chronic Effects/Carcinogenicity: 3.5 OSHA Regulatory Status:

First Aid Measures


4.1 Eyes: 4.2 Skin: 4.3 Ingestion: 4.4 Inhalation: In case of contact with eyes, flush thoroughly with water for at least 15 minutes. Call a physician if irritation develops. In case of contact with skin, wash the affected area with water for at least 15 minutes. Call a physician if irritation develops. Dilute with water or milk. Do not induce vomiting. Call a physician if necessary. If inhaled, remove person to fresh air. Give artificial respiration if necessary. If breathing becomes difficult, give oxygen. Wash thoroughly with soap and water. Allow to bleed freely. Call a physician. Not Applicable.
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4.5 Puncture Wounds:


4.6

Note to Physician:

MSDS-07060 Rev. 3

Material Safety Data Sheet


3% Acetic Acid with Methylene Blue Cat. No: 07060

Fire Fighting Measures


5.1 Flash Point/Method: 5.2 Explosive Limits: 5.2.1 Upper: 5.2.2 Lower: 5.3 Autoignition Temperature: 5.4 Hazardous Combustion Products: 5.5 Conditions of Flammability: 5.6 Extinguishing Media: 5.7 Fire Fighting Procedures: 5.8 Explosion Data: 5.8.1 Sensitivity to Mechanical Impact: 5.8.2 Sensitivity to Static Discharge: Not Available. Not Available. Not Available. Not Available. Not Available. Not Available. Not Available. Water spray, dry chemical powder, or appropriate foam. Not Available. Not Available.

Accidental Release Measures


6.1 Leak and Spill Procedure: Wear chemical-resistant gloves. Absorb spill with inert material and place in closed container for disposal. Ventilate and wash area thoroughly after clean-up is complete.

Handling and Storage


7.1 Handling: 7.2 Storage: Should be handled by trained personnel observing good laboratory practices. Avoid breathing vapor. Avoid contact with eyes and skin. Store at room temperature (15-25C).

Exposure Controls/Personal Protection


8.1 Engineering Controls: 8.2 Personal Protective Equipment: 8.2.1 Respiratory Protection: 8.2.2 Eye Protection: 8.2.3 Skin Protection: 8.3 General Hygiene Considerations: 8.4 Exposure Limits: 8.4.1 ACGIH TLV-TWA: 8.4.2 OSHA PEL-TWA: Not Available. Not Available. This is a laboratory-use product for which no industrial protective equipment has been designated. Safety glasses. Lab coat, chemical resistant gloves. Wash hands after use. Use with adequate ventilation.

Physical/Chemical Properties:
9.1 Appearance: Clear, blue liquid
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MSDS-07060 Rev. 3

Material Safety Data Sheet


3% Acetic Acid with Methylene Blue
9.2 Odor: 9.3 Physical State: 9.4 pH: 9.5 Boiling Point: 9.6 Melting Point: 9.7 Freezing Point: 9.8 Vapor Pressure: 9.9 Vapor Density: 9.10 Specific Gravity: 9.11 Evaporation Rate: 9.12 Solubility in Water: 9.13 Odor Threshold: Vinegar-like odor Liquid 2.4 - 4 Approximately 100C Approximately 0C Not Available Not Available Not Available Approximately 1 Not Available Infinite Not Applicable Not Available.

Cat. No: 07060

9.14 Coefficient of Water/Oil Distribution:

10 Stability/Reactivity:
10.1 Chemical Stability: 10.2 Conditions to Avoid: 10.3 Incompatibility (Material to Avoid): 10.4 Hazardous Decomposition/By-Products: 10.5 Hazardous Polymerization: Stable Not Available. Strong bases When heated to decomposition it emits irritating fumes. Will not occur.

11 Toxicological Information
11.1 Effects of Short-Term Exposure: 11.2 Effects of Long-Term Exposure: 11.3 Irritancy of Product: 11.4 Sensitization to Product: 11.5 Carcinogenicity: 11.6 Reproductive Toxicity: 11.7 Teratogenicity and Embryotoxicity: 11.8 Mutagenicity: 11.9 Name of Toxicologically Synergistic Products: 11.10 LD50 (specify species and route): 11.11 LC50 (specify species): Not Available Not Available Not Available Not Available Not Available Not Available Not Available Not Available Not Available Oral, Rat: 3310 mg/kg; Dermal, Rabbit: 1.06 g/kg Inhalation, Mouse: 5620 ppm/1 hour

MSDS-07060 Rev. 3

December 2012

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Material Safety Data Sheet


3% Acetic Acid with Methylene Blue 12 Ecological Information
12.1 Acetic Acid has high biochemical oxygen demand and a potential to cause oxygen depletion in aqueous systems, low potential to affect aquatic organisms and a low potential to affect the growth of some plant seedlings.

Cat. No: 07060

13 Disposal Considerations
13.1 Waste Disposal Method: Disposal should be in accordance with existing practices at your institution. Observe all Federal, Provincial, and Local Laws.

14 Transport Information
14.1 Transport Canada 14.1.1 PIN No.: Not Available.

14.2 U.S. Department of Transportation: 14.2.1 14.2.2 14.2.3 14.2.4 14.2.5 Proper Shipping Name: Hazard Class: ID. Number: Packing Group: Label Statement: Not Available. This substance is not known to be hazardous for transport. Not Available. Not Available. Not Available.

15 Regulatory Information
15.1 WHMIS Classification: 15.2 Note: B3, E This MSDS was prepared according to the Canadian Controlled Products Regulation and contains all of the information required by those regulations.

16 Other Information
16.1 Preparation Information: Refer to PIS No. 07060. 3.1, 3.2, 3.3, 4.1, 4.2, 4.3, 4.4, 7.1, 8.2.3, 9.4, 9.6, 9.7, 9.10, 9.12, 10.3, 10.4, 10.5, 11.10, 11.11, 12.1, 15.1 16.2 This MSDS has been revised in the following section(s):

16.3 Original Issue Date: 16.4 Notice:

October 5, 1993

The above information is believed to be correct but does not purport to be all inclusive and shall be used only as a guide. StemCell Technologies Inc., shall not be held liable for any damage resulting from handling or from contact with the product. The information contained in this Material Safety Data Sheet (MSDS) is current as of the Date Prepared shown in Section 1.7 of this document and may be subject to ammendment by StemCell Technologies Inc.

16.5 Disclaimer: THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT USED FOR DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

MSDS-07060 Rev. 3

December 2012

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