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Principal lipids that have metabolic significance are triacyl glycerol (TG) phospholipids and steroids, chief of which is cholesterol. TG derived from intestinal absorption of fats are transported in the blood as a lipoprotein complex called "chylomicrons" NEFA is now known to be metabolically most active of the PLASMA LIPIDS and 1 / 2 life being 2-3 minutes.
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PLASMA LIPIDS 1- In Mammals, Principal Lipids That Have Metabolic
Principal lipids that have metabolic significance are triacyl glycerol (TG) phospholipids and steroids, chief of which is cholesterol. TG derived from intestinal absorption of fats are transported in the blood as a lipoprotein complex called "chylomicrons" NEFA is now known to be metabolically most active of the PLASMA LIPIDS and 1 / 2 life being 2-3 minutes.
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Principal lipids that have metabolic significance are triacyl glycerol (TG) phospholipids and steroids, chief of which is cholesterol. TG derived from intestinal absorption of fats are transported in the blood as a lipoprotein complex called "chylomicrons" NEFA is now known to be metabolically most active of the PLASMA LIPIDS and 1 / 2 life being 2-3 minutes.
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Attribution Non-Commercial (BY-NC)
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Descărcați ca DOC, PDF, TXT sau citiți online pe Scribd
1- In mammals, principal Lipids that have metabolic significance are as follows:
• Triacyl glycerol (TG): also called Neutral fats (NF) • Phospholipids and • Steroids: chief of which is cholesterol. Plasma lipids also constitute the products of the metabolism. • Fatty acids: long-chain and short-chain (free FA) and • Glycerol. 2-Extraction of plasma lipids with a suitable Lipid solvent and subsequent separation of the extract into various classes of lipids shows the presence of: • Triacyl glycerol (TG) • Phospholipids (PL) Approximately in equal quantities • Cholesterol and • A much smaller fraction of non-esterified long-chain fatty acid (NEFA) or free-fatty acid (FFA), which constitutes less than 5% of total FA present in plasma. 3-NEFA is now known to be metabolically most active of the plasma lipids and ½ life being 2-3 minutes. 4- Plasma lipids at any time may be considered to represent the net balance between production, utilization and storage.
Table showing lipid fractions in plasma
Lipid fractions Plasma level in mg/100ml Range Mean Total lipids 360-820 560
FA(NEFA) TRANSPORTATION OF PLASMA LIPIDS Principal Lipid, triacyl glycerol (TG), is hydrophobic material. To transport them in blood in an aqueous medium poses a problem, which is solved by associating more insoluble lipids with more "Polar" ones, such as phospholipids, cholesterol and combining with a specific, protein molecule (called as 'apo-proteins')- Thus, the hydrophobic and insoluble triacyl glycerol (TG) is converted by above combination into a hydrophilic and "soluble" Lipoprotein "complex". Thus: • TG derived from intestinal absorption of fats are transported in the blood as a lipoprotein complex called "chylomicrons". Chylomicrons are small microscopic particles of fats, about 1u in diameter and are responsible for transport of exogenous (TG) in the blood. • Similarly, TG that are synthesized in Liver cells are converted to lipoprotein particles, called "very low density lipoproteins" (VLDL) and thrown into the circulation. VLDL is mainly concerned with transport of endogenous TG. In addition to above, • Fatty acids released from adipose tissue by hydrolysis of TG are thrown in the circulation as free fatty acid (FFA). They are carried in non-esterified state in plasma hence also called NEFA. In circulation, FFA/ NEFA combines with albumin and are carried as "albumin-FFA complex". Some 25 to 30 mols of FFA are present in combination with one mol. of albumin. SEPARATION OF PLASMA LIPIDS (a) Ultracentrifugation: Pure fat is less dense than water. As the proportion of lipid to protein in lipoprotein complex increases the density of the molecule decreases. This property has been utilized in separation of plasma lipids, the various lipoprotein fractions, by ultracentrifugation. Sf Unit: The rate at which each lipoprotein fraction floats up through a solution of NaCl (Sp. gr = 1.063) is expressed in "Svedberg units" (Sf units). One Sf unit is equal to 10r13 cm/sec/dyne/gm at 26°C. Because of the low densities, the chylomicrons and VLDL rise to the surface most rapidly upon ultracentrifugation and hence have relatively high Sf values. Composition of various Lipoprotein fractions separated by ultracentrifugation is given in Table 21.2. (b) Electrophoresis: Lipoproteins may be separated also according to their electrophoretic properties and identified more accurately using immunoelectro-phoresis. Fredrickson and others (1967) identified lipoproteins into 4 groups by electrophoresis as follows: • HDL: moves fastest and occupies position of a globulin-called a lipoproteins • LDL: β-lipoproteins • VLDL: (Pre-β or α 2 lipoproteins) and • Chylomicrons: slowest moving and remains near the origin.
Composition of lipoprotein complexes and apoproteins (Refer, Lipoproteins metabolism)
Table 21.3 shows the normal value of Lipoprotein fractions in health. METABOLISM OF ADIPOSETISSUE The lipids in the body physiologically exist in two forms: • "Element constant" or structural lipids and • "Element variable": stored lipids (Depot fats). Although a sharpline of demarcation cannot be made between the two, it has been generally observed that the value of the former remains constant even under extremes of starvation, whereas the latter varies. Composition of Element Constant: Cytoplasm and cell membranes of all organs are composed of "element constant", so that their fat content does not diminish in starvation. Element constant is composed chiefly of Phospholipids (PL), along with smaller amounts of other lipids, including cholesterol. It is independant of previous feeding. It remains an integral part of cell protoplasm and is essential for its life. Composition of Element Variable: The lipids which is stored in the body in excess of above. The amount fluctuates and it is composed mainly of triacyl glycerol (TG), also called as neutral fats (NF). Thus depot fat is chiefly composed of glycerides of various fatty acids and usually contains 75% of oleic acid, 20% of palmitic acid and 5% of stearic acid. Traces of lecithin and cholesterol as well as a little amount of Polyunsaturated FA are also present. The depot fat is called "adipose tissue", they are intracelhilar fats which remain inside the cells of adipose tissue. TABLE 21.3: NORMAL VALUES OF LIPOPROT (NORMAL LIPID PROFILE) Lipid fraction Normal value Total cholesterol Serum HDL—cholesterol Serum TG (Triacyl glycerol) Serum chylomicrons Serum pre-p lipoproteins (VLDL) Serum p-lipoproteins (LDL) "Serum LDL—cholesterol 150 to 240 mg/d males—35 to 60 female—40 to 7C males—60 to 16! females—40 to 1 up to 28 mg/dli post-absorptive males —up to 24 females—up to', up to 550 mg/d up to 190 mg/d "Serum LDL—cholesterol can be calculated by the Friedeii • LDL—cholesterol in mg/dl = T/"1
Total cholesterol—HDL cholesterol— -———
• LDL—cholesterol in m.mol/L = TG Total cholesterol—HDL cholesterol— —- Note: The formula is not much reliable at TG co > 4.5 m. mol/L (> 400 mg/dl). Dynamic State of Adipose Tissue: Adipose t just a static lump of fats; it is in "dynan breakdown of fats and synthesis take place a METABOLISM TG stores in the body is continually und< Esterification (synthesis) and (b) Lipolysis (bi These two processes are not the forward, processes of the same reaction. They are entinboys involving different reactants and enzymes. iyof the nutritional, metabolic and hormonal factors ilate either of these two mechanisms, i.e., esterification llipolysis. Resultant of these two processes determine '.magnitude of free fatty acid pool in adipose tissue I this, in turn, will determine the level of free fatty i(FFA) circulating in the blood. Esterification (Synthesis of TG): In adipose tissue, lor TG synthesis two substrates are required: v • AcylCoA ' a- Glycero-P Acyl-CoA a-Glycero-P 1
1 TG
For detailed steps see biosynthesis of TG.
, Sources ofAcyl Co A: Sources of FFA in blood are: • Dietary, • Synthesis of FA (palmitic acid) from acetyl CoA-'de novo' synthesis (extramitochondrial). Further elongation to form other fatty acids in microsomes. • Acyl CoA obtained from lipolysis taking place in adipose tissue (FFA-Pool No. 1). ' FFA obtained from lipolysis of TG of circulating chylomicrons and VLDL by lipoprotein lipase enzyme present in capillary wall (FFA-Pool No. 2), which are taken up by adipose tissue. 1 Source ofa-Glycerol-P: Mainly two: • Conversion of glycerol to a-Glycero-P by the enzyme Glycerokinase in presence of ATP. • The other source is from glucose oxidation. Dihydroxyacetone-P is converted to a-Glycero-P. The enzyme glycerokinase is practically absent in idipose tissue. If any glycerokinase is present, it has very low activity. Hence glycerol produced by lipolysis in idipose tissue cannot be utilized for provision of a- Glycero-P and thus glycerol passes into the blood, from where it is taken up by liver, kidney and other tissues which possess glycerokinase and is utilized for jluconeogenesis. Thus, for provision of a-glycero-P in idipose tissue for TG. Synthesis, the tissue is dependant wiasupply of glucose and glycolysis. 11, Lipolysis (Breakdown of TG): TG in adipose tissue undergoes hydrolysis by a hormone-sensitive TG lipase enzyme to form free fatty acids and glycerol. h&polytic Upases are three: \. "Hormone sensitive" triacyl glycerol lipase, key regulating enzyme. 2, Two others are not hormone-sensitive, > Diacyl glycerol lipase • Monoacyl glycerol lipase. These Upases are distinct from lipoprotein lipase that catalyzes lipoprotein TG (Present in chylomicrons and VLDL) hydrolysis before it is taken up by extrahepatic tissues. The free fatty acids formed by lipolysis can be reconverted in the tissue to acyl Co A by Acyl- CoAsynthase and re-esterified with a-glycero-P to form TG. Thus, there is a continuous cycle of lipolysis and reesterification within the tissue. TG Hormone sensitive TG Lipase 'a' Diacyl glycerol + FFA FFA Monoacyl glycerol lipase Diacyl glycerol lipase Mono acyl glycerol + FFA GLYCEROL 4 Diffuses out of plasma Note: When the rate of re-esterification is less than rate of lipolysis, FFA accumulates and diffuses into the plasma where it raises the level of FFA T in plasma. Effect of Glucose: Under conditions of adequate nutritional intake or when utilization of glucose by adipose tissue is increased, then more a-glycero-P will be available. Re-esterification will be greater than lipolysis; as a result FFA outflow decreases and plasma FFA I level falls. In vitro studies have shown that release of glycerol continues; that means lipolysis continues. Hence effect of glucose in reducing plasma FFA level is not mediated by decreasing the rate of lipolysis. It proves that the effect is due to provision of a-glycero-P from glycolysis, which enhances esterification. Adipose tissue metabolism in Diabetes mellitus and in starvation: In diabetes mellitus and in starvation, availability of glucose in adipose tissue is grossly reduced, resulting to lack of a-glycero-P. Thus rate of re-esterification is decreasedl. Lipolysis is greater than re-esterification, resulting to accumulation of FFA and increase in plasma FFA level. INFLUENCE OF HORMONES ON ADIPOSETISSUE Rate of release of FFA from adipose tissue, is affected by many hormones which influence either the (a) rate of esterification or (b) the rate of lipolysis. I. List of hormones that increase the rate of esteri- fication: • Insulin is the principal hormone • Prolactin effective in large doses. •.land is intact. Exact nature of the substance yet to be kidated. 1SSIMILATION OF TGFA BY ADIPOSE TISSUE: lajor chemical forms in which plasma lipids interact with ilipose tissue is TG. 1 As 'chylomicrons' derived from intestinal absorption of fats. 1 As "very low-density lipoprotein" complex (VLDL) by Liver. TG of circulating chylomicrons and VLDL is acted iponby the enzyme Lipoprotein lipase to hydrolyze TG to im FFA and glycerol. Significant correlation between ility of adipose tissue to incorporate TG FA and the Etivity of the enzyme Lipoprotein lipase has been found IB! the activity varies with the nutritional and hormonal •ate.Activity of lipoprotein lipase in adipose tissue is high j the fed state and 'Low' in starvation and Diabetes idlitus. laracteristics of Lipoprotein Lipase: 1 The enzyme lipoprotein lipase is located in, walls of blood capillaries. The enzyme remains bound to wall byproteoglycan chains of Heparan-SO4Has been found in extracts of heart, adipose tissue, spleen, lungs, renal medulla, aorta, diaphragm and lactating mammary gland. Normal blood does not contain appreciable quantities of the enzyme. However, following injection of Heparin lipoprotein lipase is released from its binding with Heparan SO4 into the circulation and is accompanied by clearing of lipaemia (hence called as "clearingfactor"). Another lipase is also released from Liver, a hepatic lipase by large quantities of heparin, but this enzyme has properties different from those of lipoprotein lipase and does not react readily with TG of chylomicrons and VLDL. Both phospholipids (PL) and apolipoprotein-CII are required as cofactors for lipoprotein lipase activityApo-C-II contains a specific PL binding site through which it is attached to the lipoprotein. Thus, the chylo-microns and VLDL provide the enzyme for their metabolism with both its substrate and cof actors. Hydrolysis takes place while the lipoproteins are attached to the enzyme on the endothelium. TG is progressively hydrolyzed to give DG and then MG and finally glycerol and FFA (three molecules). Some of hydrolyzed FA return to circulation being carried by albumin and bulk of FFA is taken up by tissues including adipose tissue (FFA-Pool-2). Role of Hormones: In adipose tissue, Insulin enhances the synthesis of lipoprotein lipase in adipose tissue cells and its translocation to lumirial surface of capillary endothelium. BROWN ADIPOSETISSUE Types of Storage Fats: There are two types of storage fats: • Storage "white" fat present in depot fats-predominant • In addition to usual white storage fat, another type of "pigmented" brown fat is stored in some species including humans. Role in Thermogenesis: 1. Brown adipose tissue is involved in metabolism particularly at times when a heat generation is necessary. Thus, the tissue is extremely active • In arousal from hibernation, • In animals exposed to cold, and • In heat production in newborn animals. It is present in rats, throughout the life. 2. Though not a prominent tissue in humans, recently it has been shown to be active in normal individuals, where it appears to be responsible for "diet-induced thermogenesis", which may account for how some persons can "eat and do not get fat". Note: It is to be noted that brown adipose tissue is reduced or may be absent in obese persons. Location: It is located and present particulary in the thoracic region. Characteristics of Brown Adipose Tissue: It is characterized by: • A high content of mitochondria, • A high content of cytochromes, • A well-developed blood supply, • Also relatively rich in carnitine, whid for FA oxidation, • Unlike white adipose tissue, it ha; glycerokinase. Note: • The brown colour is related to a relatrv chrome content. • There is lowATP-synthase activity. • Oxygen consumption is high. Metabolic Peculiarities: 1. Metabolic emphasis is on oxidativ oxidation of glucose and FA oxidatic oxidized to CO2 and H2O. 2. Nor-epinephrine liberated from symf endings is important in increasing lip tissue. Mechanism of Heat Production: • Oxidation and phosphorylation are n< mitochondria of this tissue. Dinitropl effect and there is no respiratory contro • The phosphorylation which occurs is at t level" in the glycolytic pathway and i thiokinase" step of TCA cycle. Thus, o; duces much heat and very little free ener as ATP due to decreased coupling of o; phosphorylation. • In terms of chemi-osmotic theory, it app proton gradient, normally present aero mitochondrial membrane of coupled m is continually dissipated in brown adipos thermogenic protein, called "thermoge acts as a proton conductance pathway membrane. This explains the apparent la of uncouplers. Function: Brown storage fat has a somev temperature than other tissues. It plays a: production for vital organs, serving as a sort pad" or "furnace" for the local application o the vital organs of the thorax, the upper spin, the autonomic sympathetic chain. The amou fat increases in animals when subjected to "c Direct measurements of heat production d stress in rats showed that brown adipose tissi for 82% of total heat production. Study the metabolic fate of cholesterol in the body. What are bile acids? Learn how they are formed in the body and their functions. Study the role of cholesterol and TG in atherosclerosis and IHD. OXIDATION OF FATTY ACIDS Sources of Plasma FFA Plasma free fatty acids are derived: • Mainly from lipolysis in adipose tissue. (Pool-1) • Portion of FFA is derived from degradation of circulating chylomicrons and VLDL by the action of the enzyme lipoprotein lipase (Pool-2). • A small portion of plasma FFA is derived from absorption of dietary source specially small chain and medium chain fatty acids. • Also FFA is obtained from synthesis from acetyl CoA in Liver cells, which are incorporated in TG. In postabsorptive state, plasma contains 10 to 30 mg% of FFA, most of which is transported in plasma as a loose complex with albumin, as "albumin-fFA complex" but in the cell they are attached to a fatty acid binding protein or "Z-Protein". A small amount of FA is also associated with HDL. Shorter chain FA are more water soluble and exist as the unionized acid or as a FA anion. Fatty acids exhibit a very rapid turnover rate with a l/2 life of only 1 to 3 minutes, they are rapidly taken up by tissues and metabolized. Plasma FFA is decreased by Insulin and Glucose administration. Plasma FFA is increased by catecholamines, Growth hormone, Glucocorticoids and thyroid hormones. Also increased in Diabetes mellitus, starvation and with high fat diets. Methods by which fatty acids are oxidized in the body are as follows: A. beta-oxidation: Principal method of oxidation of FA. Other methods are: B. alpha-oxidation, C. OMEGA-oxidation, and D. Peroxismal FA oxidation. A. BETA-OXIDATION Principal method by which FA are oxidized is called beta-oxidation. Several theories have been proposed to explain the mechanism of the oxidation of FA chains. The classical theory of P-oxidation was the outcome of the work of Knoop. Knoop's Experiment: Tagged the -CH3 end of FA by substitution of a phenyl radical, this prevented the complete oxidation. It resulted in urinary excretion of phenyl derivatives: • On feeding dogs with FA with even carbon atoms,he observed that phenyl acetic acid is always is conjugated with glycine and excreted as phenylaceturic acid. • When FA with odd number of carbon at Benzoic acid was excreted as gly< "hippuric acid". Conclusion: Knoop proposed the p-ox According to this mechanism FA chains. the removal of 2 carbon atoms at a time, in the p-position to COOH group is attacked with the formation of the corresf acid; then the two terminal C-atoms are spliti CoA". A new -COOH group is formed at 1 keto (= CO) grouping, so that a fatty acidi carbon atoms less than the original. Aga carbon atom is attacked and two more ca split off as acetyl-CoA. In this way, the FA i the removal of 2 carbon atoms at a time, i stage of acetoacetic acid is reached. Tissues in which ^-Oxidation is Carried i lating FA are taken up by various tissues, Tissues like liver, heart, kidney, muscle, bra and adipose tissue have the ability to oxic FA. In cardiac muscle, fatty acids are an ir respiration (80% of energy derived from F/ Enzymes Involved in ^-Oxidation: fl-oi place in mitochondrion. Several en2 collectively as EA- oxidase system are fov chondrial matrix, adjacent to the respiratory! is found in the inner membrane. These er the oxidation of FA to acetyl-CoA. Activation of FA: Fatty acids are in cyt (extramitochondrial). Fatty acids must be i so that they participate in metabolic activation requires energy which is provi presence of ATP, and coenzyme A, the er synthetase (previously called as thiokin conversion of a free fatty acid to an 'active"! The presence of inorganic pyrophosph activation goes to completion by facilit additional high energy ~ P bond of PPi. effect 2 - P bonds are expended during H of each FA molecule. Not only saturated FA I FA and -OH fatty acids are also activated ZoA synthetases. I types ofAcyl-CoASynthetases: The enzymes i the endoplasmic reticulum and inside (for and outside (for long-chain FA) of the i. Several varieties of the enzyme have been |,each specific for FA of different chain lengths. CoA synthetase —> acts on acetic acid and :acid Iwifdium chain synthetase -» acts on FA with igth C4 to C12 JIM acyl-CoA synthetase ~^> Acts on FA with gth C8 to C22 a GTP-specific mitochondrial acyl-CoA ase described which forms GDP + Pi ! AND ITS ROLE IN FA METABOLISM L (acyl-CoA) are formed in cytosol, whereas i of FA occurs in mitochondrial matrix. Acyl neable to mitochondrial membrane. Long-ited FA penetrate the inner mitochondrial eonly in combination with carnitine. tChemistry and functions: s chemically "p-OH-y-trimethyl ammoniumHistorical background: Fraenk^l's vitamin BT is same as carnitine. It was found to be required as a nutritional factor in meal-worm (Tenebrio molitor). If the meal worms are fed on a synthetic diet, deficient in vitamin BT they die in 4 to 5 weeks. Distribution: Carnitine is widely distributed in yeast, milk, liver and particularly large quantities in muscles and in meat extracts. Concentration: Fraenkel used bio-assay technique based on the rate of growth of larvae of Tenebrio molitor and found that in mammals, carnitine content of: • Skeletal muscle: 1 mg/Gm dry weight • Heart muscle: 560 mcg/Gm • Kidneys: 412 mcg/Gm • Liver: 280 mcg/Gm Blood: Small amounts in blood 7-14 meg/ml. Excretion in 24 hrs urine: 50 to 100 meg/ml. Biosynthesis of Carnitine: It is synthesized from lysine and methionine in liver principally, also in kidneys. Synthesis of carnitine is shown below in the box. Biosynthesis of CarnitineHistorical background: Fraenk^l's vitamin BT is same as carnitine. It was found to be required as a nutritional factor in meal-worm (Tenebrio molitor). If the meal worms are fed on a synthetic diet, deficient in vitamin BT they die in 4 to 5 weeks. Distribution: Carnitine is widely distributed in yeast, milk, liver and particularly large quantities in muscles and in meat extracts. Concentration: Fraenkel used bio-assay technique based on the rate of growth of larvae of Tenebrio molitor and found that in mammals, carnitine content of: • Skeletal muscle: 1 mg/Gm dry weight • Heart muscle: 560 mcg/Gm • Kidneys: 412 mcg/Gm • Liver: 280 mcg/Gm Blood: Small amounts in blood 7-14 meg/ml. Excretion in 24 hrs urine: 50 to 100 meg/ml. Biosynthesis of Carnitine: It is synthesized from lysine and methionine in liver principally, also in kidneys. Synthesis of carnitine is shown below in the box. Biosynthesis of CarnitineSuccinate Functions: Carnitine is considered as a "carrier molecule"; it acts like a ferry-boat. It transports long-chain acyl-CoA across mitochondrial membrane which is impermeable to acyl-CoA. • Facilitates transport of long-chain acyl-CoA for oxidation in mitochondria. • Facilitates exit of acetyl-CoA and acetoacetyl CoA from within mitochondria to cytosol, where FA synthesis takes place • Methionine-sparing action: Khairallah and Wolf (1965) found that in rats, carnitine has a methionine-sparing action and may thus be considered a food factor required in marginal diets. Mechanism of Transport of Long-Chain Acyl-CoA: Activation of lower FA and their oxidation may occur within the mitochondria, independantly, of carnitine; but long-