SELECTIVE CULTURE MEDIA 1. Bile Esculin Medium (BEM) 2. Bismuth Sulfite Agar (BSA) / Wilson Blair Agar 3.

Bordet Gengou Agar/ Potato Blood Glycerol Agar 4. Brucella Blood Agar 5. Biphasic media of Brucella Broth and Agar in Castaneda bottle or septi-check 6. Blood Cystine Glucose Agar with thiamine 7. Buffered Charcoal Yeast Extract Agar (BCYE) 8. Cefsulodin Irgasan Novobiocin (CIN) Agar 9. Campy Blood Agar 10. Campylobacter Thioglycollate Broth 11. Cefoperazone, Vancomycin, Amphotericin Medium 12. Skirrows Medium 13. Chapman-Stone Agar 14. Columbia Colistin Nalidixic Agar (CNA) 15. Cooked Meat/ Chopped Meat Medium 16. Lombard-Dowell Agar 17. Cycloserine Cefoxitine Fructose Agar (CCFA) 18. Cystine Lactose Electrolyte Deficient (CLED) Agar 19. Cystine Tellurite Blood Agar 20. Loefflers Coagulated Serum Slants 21. Tinsdale Agar 22. Pais Agar 23. McLeods Agar 24. Clostrisel Agar 25. Dieudonnes Medium 26. E agar 27. Edward Hayflick Agar 28. Ellinghausen, McCullough Johnson & Harris (EMJH) Medium 29. Fletchers semisolid medium 30. Fetal Bovine Serum Agar with Vancomycin 31. Human Blood Bilayer Tween (HBT) Agar 32. Modified Kellys Medium/ Barbour-Stoenner-Kelly (BSK-II) 33. American Thoracic Society Medium 34. Dorset Egg Medium 35. Lowenstein Jensen Agar 36. Lowenstein Jensen (LJ) Agar with antibiotics 37. Middlebrook 7H9 (liquid)/ 7H10/ 7H11 (solid) 38. Pertragnani Medium 39. Mitchison Agar Isolation of Mycobacterium spp. Isolation of Haemophilus ducreyi (Ducreys bacillus) Isolation of Gardnerella vaginalis Isolation of Borrelia spp. Isolation of Mycoplasma and Ureaplasma spp. Isolation of Leptospira interrogans Isolation of Corynebacterium perfringens (Gas bacillus) Isolation of Vibrio species Isolation of Corynebacterium diphtheria (Kleb Loefflers bacillus) Isolation of anaerobes Isolation of Clostridium difficile Isolation and enumeration of bacteria in urine Isolation of Staphylococci Isolation of Staph. and Strep. from mixed specimen Isolation of Campylobacter spp. Isolation of Francisella tularensis Isolation of Legionella pneumophila Isolation of Yersinia enterocolitica Isolation of Brucella spp. Selective for Group D Streptococcus and Enterococcus Isolation of Salmonella typhi (Typhoid bacillus) Isolation of Bordetella pertussis

40. Martin Lewis Agar 41. Modified Thayer Martin Medium 42. Thayer Martin Agar 43. New York City (NYC) Agar

Isolation of pathogenic Neisseria species (e.g. Neisseria gonorrhoeae)

Isolation of pathogenic Neisseria species (e.g. Neisseria gonorrhoeae), Mycoplasma hominis and Ureaplasma urealyticum

44. McBride Medium 45. Wallenstein Medium

Isolation of Listeria monoctyogenes Isolation of non-tuberculous mycobacteria (e.g. M. Avium)

46. Phenylethanol Agar (PEA) 47. Streptococcal Selective Agar 48. Cetrimide Agar Slant 49. Shepards A7-B Agar

Isolation of Gram (+) cocci and anaerobic Gram (-) bacilli Isolation of Strep. pyogenes and Strep. agalactiae Isolation and identification of Pseudomonas aeruginosa Isolation of Mycoplasma species

ENRICHMENT CULTURE MEDIA 1. Alkaline Peptone Water 2. Chocolate Agar Increase the number of pathogenic vibrio in the specimen Enriched with heated blood for the cultivation of most organisms (esp. Haemophilus spp. & Neisseria spp.) 3. Dextrose Ascitic Fluid Agar 4. Gram Negative Broth (GN) 5. Selenite F Broth 6. Tetrathionate Broth Isolate organisms in CSF Increase the number of pathogens in stool Enrichment and isolation of Salmonella spp. Isolation of Samonella and Shigella spp. From stools

REDUCING/ANAEROBIC CULTURE MEDIA 1. Anaerobic Blood Agar 2. Anaerobic Phenylethylalcohol (PEA) Agar Isolation of all anaerobes Isolation of obligate Gram (+) & (-) anaerobes which inhibits Growth of enteric bacilli and swarming Proteus 3. Anaerobic Kanamycin Vancomycin Blood Agar Isolation of Gram (-) anaerobes (esp. Bacteroides, Prevotella and Fusobacterium) 4. Anaerobic Paroromycin Vancomycin Laked Blood Agar Isolation of Gram (-) anaerobes (esp. Bacteroides, Prevotella and Fusobacterium) Detection of pigment production of Prevotella

TRANSPORT CULTURE MEDIA 1. Amies Medium 2. Cary & Blair Medium 3. Stuarts Medium To maintain the variability of the organism from the source to the laboratory

BIOCHEMICAL CHARACTERISTIC CULTURE MEDIUM 1. Cystine Trypticase Agar (CTA) Identification of Nisseria ssp. based on their ability to utilize carbohydrate 2. Litmus Milk Medium 3. Oxidation-Fermentation (O-F) Medium Isolation of Clostridium perfringens Differentiation of organisms based on their ability to oxidize or ferment carbohydrates 4. Lysine Iron Agar (LIA) Identification of Gram (-) enteric bacilli based on their ability to: decarboxylate/ deaminate lysine and to produce hydrogen sulfide or not 5. Moellers Broth Identification of Gram (-) enteric bacilli based on their ability to: produce LDL/ODC/ADH 6. Tryptone Broth Identification of Gram (-) enteric bacilli based on their ability to: produce indole 7. Triple Sugar Iron (TSI) Agar Identification of Gram (-) enteric bacilli based on their ability to: ferment glucose, lactose and sucrose and produce gas and hydrogen sulfide 8. Methyl Red Voges Proskauer (MRVP) Broth/ Peptone Glucose Broth Identification of Gram (-) enteric bacilli based on their ability to: ferment glucose via mixed acid fermentation or butylene glycol pathway 9. Christensen Urea Agar/ Stuart Urea Broth Identification of Gram (-) enteric bacilli based on their ability to: produce enzyme urease 10. Phenylalanine Deaminase Agar Identification of Gram (-) enteric bacilli based on their ability to: produce enzyme deaminase 11. Simmon Citrate Agar Identification of Gram (-) enteric bacilli based on their ability to: utilize citrate as source of carbon 12. Acetamide Slant Identification of organisms which utilize acetamide as a source of carbon (e.g. Pseudomonas aeruginosa) 13. Acetate Slant Identification of organisms which utilize acetate as a source of carbon (e.g. Escherichia coli) 14. PYR agar Differentiate hemolytic Strepococci & Enterococci Based on their ability to produce enzyme pyrrolidonylaryamidase or pyroglutamylaminopeptidase

SELECTIVE + DIFFERENTIAL CULTURE MEDIUM 1. Bacteroides Bile Esculin (BBE) Selective for Bacteroides sps. Differentiate Bile resistant from Bile sensitive Bacteoides sps. 2. Eosin Methylene Blue Agar (EMB) 3. MacConkey Agar 4. Hektoen Enteric Agar (HEA) Selective for Gram (-) bacilli Differentiate lactose from non-lactose fermenting Gram (-) enteric bacilli Selective for Gram (+) bacilli Differentiate lactose from non-lactose fermenting Gram (-) enteric bacilli 5. Mannitol Salt Agar (MSA) 6. Salmonella-Shigella Agar Isolation of mannitol and non-mannitol fermenting Staph. Isolation and differentiation of Salmonella and Shigella sps. Isolation and Differentiation of Vibrio spp. Identification of Gram (-) enteric bacilli Differentiation of Salmonella and Shigella sps.

7. Thiosulfate Citrate Bile Salts Sucrose (TCBS) Agar 8. Xylose Lysine Desoxycholate (XLD) Agar

BASIC/GENERAL PURPOSE MEDIUM 1. Brain Heart Infusion (BHI) Broth/Agar Basic medium for the isolation of microorganisms Used for preparing pure culture for antibiotic susceptibility test 2. Trypticase Soy Broth/Agar Supports growth of many non-fastidious and fastidious bacteria Used as a base in preparing blood agar

LABORATORY DIAGNOSTIC TEST MEDIUM 1. DNA Agar 2. Egg Yolk Agar (EYA) 3. 12% Gelatin Medium Identify Staph. aureus based on its ability to produce DNAse enzymes Identify Clostridium perfringens based on its ability to produce lecithinase identification of organisms which liquefy/hydrolyze gelatin

TISSUE CELL CULTURE 1. McCoy Cells 2. HeLa Cell Line

3. Embryonated egg

Isolation of Chlamydia

Isolation of Rickettsiae

OTHER CLUTURE MEDIUMS A. ENRICHED + DIFFERNETIAL a. Blood Agar Enriched with unheated blood Differentiates organisms thru hemolytic patterns B. DIFFERNETIAL a. Sellers Agar Differentiating non-fermentative bacilli (e.g. Pseudomonas, Acinetobacter, & Alkaligenes) C. SELECTIVE + ENRICHMENT a. Regan Lowe Agar Isolation of Bordetella pertussis b. Todd-Hewitt Broth Supplemented with antibiotics Enrichment and isolation of Strep. agalactiae D. SELECTIVE TRANSPORT a. Transgrow medium For Neisseria gonorrhoeae E. NON-SELECTIVE MEDIUM a. Schaedler Agar Recovery of anaerobes and aerobes b. Salt (NaCl) Broth Identification of organisms based on their ability to grow or not to grow in the presence of 65% NaCl F. ANTIBIOTIC SUSCEPTIBILITY TEST MEDIUM a. Mueller-Hinton Agar/Broth For antibiotic susceptibility test for most bacteria G. BIPHASIC MEDIUM a. Scotts Modified Castaneda and Thioglycollate Broth For blood culture H. GENERAL SUPPORTIVE a. Thioglycollate Broth Recovery of anaerobes, aerobes, microaerophilic and fastidious organisms I. EMPLOYED IN ANIMAL INOCULATION a. Foot pad of mice and armadillo Isolation and identification of Mycobacterium leprae (Hansens bacillus)