ABI PRISM 3100 Genetic Analyzer and

ABI PRISM 3100-Avant Genetic Analyzer

Site Preparation and Safety Guide

DRAFT
March 11, 2002 11:39 am, 3100_SPSG_TitlePage.fm

 Copyright 2002, Applied Biosystems. All rights reserved.

For Research Use Only. Not for use in diagnostic procedures.
Please refer to the ABI PRISM 3100 Genetic Analyzer and the ABI PRISM 3100-Avant Genetic ANalyzer Users Manual for limited label license or
disclaimer information.
Information in this document is subject to change without notice. Applied Biosystems assumes no responsibility for any errors that may appear in this document. This
document is believed to be complete and accurate at the time of publication. In no event shall Applied Biosystems be liable for incidental, special, multiple, or
consequential damages in connection with or arising from the use of this document.
Applied Biosystems, GeneScan, POP-4, POP-6, HI-Di, and BigDye are registered trademarks of Applera Corporation or its subsidiaries in the U.S. and
certain other countries.
AB (Design) and Applera are trademarks of Applera Corporation or its subsidiaries in the U.S. and certain other countries.
All other trademarks are the sole property of their respective owners.

Part Number 4315835 Rev. C

03/2002

DRAFT
March 11, 2002 11:39 am, 3100_SPSG_TitlePage.fm

Contents
1 Introduction
Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-1
About the Site Preparation and Safety Guide . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-1
In This Guide . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-1
About Preinstallation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-2
Preparing Your Site . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-2
Choosing a Location . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-2
About Safety. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-2
Using the Instrument Correctly . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-2
User Attention Words . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-2
About Chemical Safety. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-3
About Material Safety Data Sheets (MSDSs) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-3
About Waste Disposal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-3
Chemical Hazard Warning. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-3
Chemical Waste Hazard Warning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-4

2 Site Preparation
Before You Start . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-1
Preinstallation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-1
Operator Training . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-1
Performance Verification . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-1
Ordering Supplies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-1
Preinstallation Checklists . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-2
About These Checklists . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-2
Components Supplied by
Applied Biosystems . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-2
Equipment and Consumables Required. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-2
On-Site Personnel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-3
Laboratory Facility Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-4
Items Shipped with This Instrument. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-5
Summary List . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-5
Do Not Move or Unpack Instrument . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-5
Unpacking Chemicals . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-5
Storing the Reagents for ABI PRISM 3100 Genetic Analyzer . . . . . . . . . . . . . . . . . . . . . . . . . . 2-6
Chemistry Installation Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-6
Storing the Reagents for the ABI PRISM 3100-Avant Genetic Analyzer . . . . . . . . . . . . . . . . . . 2-8
Chemistry Installation Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-8

iii

Computer Specifications. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-9

Networking . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-9
Laboratory Safety Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-10
On-Site Representative . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-10
Required Safety Equipment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-10
Laboratory Space Required . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-11
Dimensions and Weight . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-11
Instrument Location . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-11
Helping the Engineer Move the Instrument . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-11
Typical Laboratory Layout . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-12
Laboratory Ventilation Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-13
Heat Production . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-13
System Vent . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-13
Connecting the Flexible Duct . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-13
Laboratory Environmental Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-13
Altitude . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-13
Temperature and Humidity . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-13
Pollution . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-13
Emission/Immunity Statement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-13
Electrical Requirements. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-14
Power . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-14
Power Line . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-14
Electrical Outlets. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-14
Power Rating. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-14
Power Cords . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-14
Grounding . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-14
Power Line Regulator . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-14
Voltage Spikes. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-15
Power Outages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-15
Electric Shock Warning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-15

3 Chemical Safety
Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-1
In This Chapter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-1
Material Safety Data Sheets (MSDSs) Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-2
About MSDSs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-2
Updating MSDSs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-2
Ordering MSDSs from
Applied Biosystems . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-2
Ordering MSDSs from Other Manufacturers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-2
Hazardous Chemicals . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-3
Overview. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-3

iv

Handling Hazardous Chemicals . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-3

Hazardous Waste . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-4
Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-4
Instrument Waste System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-4
Handling Chemical Waste . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-4
Storing Hazardous Waste. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-5
Disposing of Hazardous Waste . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-5

4 Instrument Safety
Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-1
In This Chapter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-1
Safe Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-1
Routine Maintenance for Safe Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-1
Instrument Labeling . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-2
Safety Labels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-2
Signal Words . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-2
Labels that May Be Found on the Instrument . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-3
Safety Alert Symbols . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-4
Electrical Symbols . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-4
Nonelectrical Symbols. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-4
Safety Alert Symbols (French). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-5
Safety Alert Symbols (German) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-6
Safety Alert Symbols (Italian) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-7
Saftey Alert Symbols (Portuguese) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-8
Safety Alert Symbols (Spanish) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-9
Safety Alert Symbols (Chinese) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-10
Safety Alert Symbols (Japanese) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-11
Safety Alert Symbols (Korean) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-12
Safety Alert Symbols (Thai). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-13
Input/Output Connection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-14
Ethernet Connection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-14
Laser Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-15
Laser Type and Class . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-15
Laser Radiation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-15
Laser Safety Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-15

A Acronyms and Abbreviations

Acronyms and Abbreviations Used in MSDSs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .A-1
Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .A-1
Organizations, Regulations, and Scientific Terminology . . . . . . . . . . . . . . . . . . . . . . . .A-1
Units of Measure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .A-2

Chemicals . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . A-3

B Technical Support
Services & Support . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . B-1
Applied Biosystems Web Site. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . B-1

vi

Introduction

Overview
About the Site A Site Preparation and Safety Guide is sent to all customers who have purchased an
Preparation and Applied Biosystems instrument. This guide provides the information you need to fully
Safety Guide prepare your site for the arrival and installation of the instrument. Complete
preparation helps ensure a smooth installation process, as well as correct and safe
instrument operation.

In This Guide This guide for the ABI PRISM 3100 Genetic Analyzer and ABI PRISM 3100-Avant
Genetic Analyzer contains the following chapters and appendixes:
Title

Description

Introduction

Provides orientation to this guide and Customer Support

contact information

Site Preparation

Provides installation requirements and the

preinstallation checklists

Chemical Safety

Provides general chemical handling guidelines and

instrument waste profiles

Instrument Safety

Explains safety alert symbols on instrument and shows

instrument input and output connections

Acronyms and Abbreviations

Explains terms used in material safety data sheets

(MSDSs) and in this guide

Technical Support

Gives website for obtaining technical support

Introduction 1-1

About Preinstallation
Preparing Your Site Preinstallation checklists start on page 2-2 of this guide. An Applied Biosystems
employee will contact you to verify that everything on the checklists has been done
before making an appointment for installation.

Choosing a Location When deciding where to put the instrument, keep in mind the following:

You must be able to disconnect the main power supply to the instrument
immediately if necessary.

You must be able to comply with local, state/provincial, or national air quality
regulations while venting the exhaust from this instrument.

If possible, you should position the instrument to allow access to the back of the
instrument.

The instrument operates more efficiently in laboratories that maintain constant

temperatures.

About Safety
Using the Use this instrument as specified by Applied Biosystems. If the instrument is used in a
Instrument manner not specified by the manufacturer, the protection provided by the instrument
Correctly may be impaired.
User Attention Five user attention words appear in the text of all Applied Biosystems user
Words documentation. Each word implies a particular level of observation or action as
described below.
Note

Calls attention to useful information.

IMPORTANT Indicates information that is necessary for proper instrument operation.

! CAUTION Indicates a potentially hazardous situation which, if not avoided, may result in a
minor or moderate injury. It may also be used to alert against unsafe practices.
! WARNING Indicates a potentially hazardous situation which, if not avoided, could result in
death or serious injury.
! DANGER Indicates an imminently hazardous situation which, if not avoided, will result in
death or serious injury. This signal word is to be limited to the most extreme situations.

1-2 Introduction

About Chemical Safety

About Material Some of the chemicals used with this instrument may be listed as hazardous by their
Safety Data Sheets manufacturer. When hazards exist warnings are prominently displayed on the labels
(MSDSs) of all chemicals.
Chemical manufacturers supply a current Material Safety Data Sheet (MSDS) before
or with shipments of hazardous chemicals to new customers and with the first
shipment of a hazardous chemical after an MSDS update. MSDSs provide you with
the safety information you need to store, handle, transport, and dispose of the
chemicals safely.
We strongly recommend that you replace the MSDSs in your files each time you
receive one packaged with hazardous chemicals.
! WARNING CHEMICAL HAZARD. Be sure to familiarize yourself with the MSDSs before
using reagents or solvents.

About Waste As the generator of potentially hazardous waste, it is your responsibility to:
Disposal
Characterize the waste generated with your applications, by analysis if necessary

Ensure the health and safety of all personnel in your laboratory

Ensure that the instrument waste is stored, transferred, transported, and disposed
of according to all local, state/provincial, or national regulations.

Note Radioactive or biohazardous materials may required special handling, and disposal
limitations may apply.

Chemical Hazard ! WARNING CHEMICAL HAZARD. Some of the chemicals used with Applied Biosystems
Warning instruments are potentially hazardous and can cause injury, illness or death.

Read and understand the material safety data sheets (MSDSs) provided by the chemical
manufacturer before you store, handle, or work with any chemicals or hazardous materials.

Minimize contact with and inhalation of chemicals. Wear appropriate personal protective
equipment when handling chemicals (e.g., safety glasses, gloves, or clothing). For
additional safety guidelines consult the MSDS.

Do not leave chemical containers open. Use only with adequate ventilation.

Comply with all local, state/provincial, or national laws and regulations related to chemical
storage, handling, and disposal.

Check regularly for chemical leaks or spills. If a leak or spill occurs, follow the
manufacturers cleanup procedures as recommended on the MSDS.

Introduction 1-3

Chemical Waste ! WARNING CHEMICAL WASTE HAZARD. Wastes produced by Applied Biosystems
Hazard Warning instruments are potentially hazardous and can cause injury, illness, or death.

1-4 Introduction

Read and understand the material safety data sheets (MSDSs) provided by the
manufacturers of the chemicals in the waste container before you store, handle, or dispose
of chemical waste.

Minimize contact with and inhalation of chemical waste. Wear appropriate personal
protective equipment when handling chemicals (e.g., safety glasses, gloves, or clothing).

Dispose of the contents of the waste tray and waste bottle in accordance with good
laboratory practices and local, state/provincial, or national environmental and health
regulations.

Site Preparation

Before You Start

Preinstallation Before the ABI PRISM 3100 Genetic Analyzer or ABI PRISM 3100-Avant Genetic
Analyzer is installed, the installation site must be prepared so that the instrument can
be operated correctly and safely. Follow the requirements presented in Preinstallation
Checklists on page 2-2, to simplify the installation procedure.
.

Operator Training The training of operators is one of the primary goals of installation. Personnel
requiring training should set aside four uninterrupted hours (one half day) to work with
the Applied Biosystems service representative. If this is not possible, please call
Applied Biosystems Service Administration to reschedule the installation.

Performance Calibration of the instrument and verification of performance will be performed by an

Verification Applied Biosystems service representative during the installation.
Ordering Supplies Before installation, be sure to contact Sales Administration or your sales
representative to order the additional supplies necessary for the ongoing operation of
the instrument. The chemicals shipped with this instrument will be completely
consumed during the installation and initial testing of the instrument.

Site Preparation 2-1

Preinstallation Checklists
About These Use the following preinstallation checklists to ensure that all preparations are made for
Checklists installing your instrument. All of the personnel and supplies on these checklists are
required at the time of installation, but most of them are not supplied by
Applied Biosystems. A service representative will contact you to confirm that
everything is checked off before making an appointment for installation.

Components The following components are supplied by Applied Biosystems. Check off each item
Supplied by after performing the action.
Applied Biosystems

Date
Confirmed

Action
Received instrument(s) and inspected the crates and boxes.
Examined the packing list to verify that instrument(s), serial number(s),
and system configuration shipped are the same as those ordered.
Note Do not unpack anything except for the Chemistry Installation Kit.
(See Items Shipped with This Instrument on page 2-5.)
Reported any discrepancies in instrument serial numbers or system
configuration, or damage to the crates or boxes to your Applied
Biosystems service representative.
Read all sections of this Site Preparation and Safety Guide.
Read the Material Safety Data Sheets (MSDSs) provided with the
Chemistry Installation Kit.
Unpacked and stored contents of Chemistry Installation Kit. (See
Unpacking Chemicals on page 2-5.)

Equipment and You must supply the following items for installation. Check off each item as you verify
Consumables its availability.
Required

Date
Confirmed

Item
Safety glasses
Lab coats
Chemical-resistant disposable gloves
Benchtop microcentrifuge for 1.5-mL and 0.2-mL tubes and microtiter
plates
Vortex mixer
Micropipettes and plugged tips (Pipetman models P-20, P-200,
P-1000; or Eppendorf 110 L, 10100 L, and 1001000 L)
Eppendorf 0.2-mL and 1.5-mL tubes
Heat block or water bath at 95 C
Timer
Ice bucket
Wet ice
Deionized water

2-2 Site Preparation

On-Site Personnel Check off each item after confirming the following with your laboratory personnel.

Date
Confirmed

Action
Have available four uninterrupted hours (one half day) for in-lab training
during installation.
Designated one person to be the laboratory safety representative. This
person is familiar with laboratory safety procedures, knows the location
of all safety equipment, and must be available to the Applied
Biosystems engineer while the engineer is at your facility.
Designated three people to be present to assist the service engineer in
lifting and/or positioning the instrument.

Site Preparation 2-3

Laboratory Facility Check off each item as you verify that the instrument location meets each of the
Requirements requirements.

Date
Confirmed

Requirement

Safety
Met requirements specified in Laboratory Safety Requirements on
page 2-10.
Instrument Location
Verified a pathway from the receiving dock to the storage area (if
applicable) to accommodate the instruments 94-cm (37-in.) crated
width.
Verified a pathway from the storage area to the laboratory to
accommodate the instruments 74-cm (29.2-in.) width, unpacked
from the crate.
Laboratory space is of correct dimensions to accommodate the
system and its ventilation clearances, and is situated so that the
instrument is accessible to the installer on all four sides. See
Laboratory Space Required on page 2-11.
The computer will be no more than 3.7 m (12 ft) from the
instrument.
Laboratory bench is of the correct dimensions and weight tolerance
to accommodate the system and computer. See Laboratory Space
Required on page 2-11.
Situated the computer to allow for proper ergonomics during use.
Ventilation and Waste
Established proper handling and disposal methods for hazardous
chemical waste.
Verified that room ventilation can accommodate instrument heat
output of 6800 Btu/h (~<2000 W). (See Laboratory Ventilation
Requirements on page 2-13.)
Electrical
A dedicated 2.5-kVA power line and ground is required for the
system. This includes:

In the USA, Canada, and Japan, use a NEMA L6-30R

In Europe and Australia, use an IEC 309 receptacle
The receptacle must be within 3 m (10 ft.) of the instrument. A line
conditioner or universal power supply (UPS) on the power line may
be recommended depending on line conditions.
Standard outlets on a dedicated circuit separate from the
instrument for the computer and monitor.

2-4 Site Preparation

Items Shipped with This Instrument

Summary List The ABI PRISM 3100 Genetic Analyzer and ABI PRISM 3100-Avant Genetic Analyzer is
shipped with the following:

3100 Genetic Analyzer or 3100-Avant Genetic Analyzer instrument in one crate

3100 Packing Kit in one box
3100 Collection Software Kit in one box
Autosampler Plate Kit 96 and/or Autosampler Plate Kit 384 in one or two boxes
Sequencing Analysis Software Module and/or GeneScan Software Module in
one or two boxes

IMPORTANT These software module kits contain the chemistry installation kits. See
Unpacking Chemicals below.

Two 3100 Capillary Arrays in two boxes

The computer and monitor in two boxes
The printer (optional) in one box

Do Not Move or Do not move or unpack instrument cartons. This protects you from liability if any
Unpack Instrument damage occurred during shipping. Inspect instrument cartons, and report any damage
to your Applied Biosystems service representative.
! WARNING PHYSICAL INJURY HAZARD. Do not move or unpack instrument cartons.
This instrument is heavy. Any incorrect lifting or moving of the instrument can cause painful and
sometimes permanent back injury. The instrument may tip over if moved or unpacked
incorrectly, causing serious injury to persons in its path or damage to the instrument itself.
Unpacking the instrument will also void your warranty with Applied Biosystems.

Unpacking You must unpack the Chemistry Installation Kit, which is boxed separately from the
Chemicals instrument. Read the MSDSs supplied with the chemicals, and store the components
as specified on page 2-6.
! WARNING CHEMICAL HAZARD. Some chemicals used with Applied Biosystems
instruments are hazardous and can cause injury, illness, or death. Always read the appropriate
MSDSs before interacting with the instrument and chemicals in any way. Hazardous Chemical
Warnings are prominently displayed on the labels of all hazardous materials.

Site Preparation 2-5

Storing the Reagents for ABI PRISM 3100 Genetic Analyzer

Chemistry Unpack the ABI PRISM 3100 Genetic Analyzer Chemistry Installation Kit upon
Installation Kit receipt, and store the chemicals and reagents as indicated in the table below. The
chemicals in this kit are completely consumed during the installation and initial testing
of the instrument. There are three types of chemistry installation kits. The kit that you
receive depends on whether you ordered the Sequencing Software Module, the
GeneScan Software Module, or the Combination Software Module.
ABI PRISM 3100 GeneScan Install Kit (P/N 4315984)
Part Number
4316100

Name
3100 GeneScan Matrix

Qty

Storage Temp. (C)

Standard Set DS-30

4316144

3100 GeneScan Installation

Standard

4316355

3100 POP-4 polymer

4311320

Hi-Di formamide

20

402824

10X Buffer with EDTA

4315931

Capillary Array 16 x 36 cm

ABI PRISM 3100 DNA v3.0 Sequencing Chemistry Install Kit (P/N 4315983)
Part Number

Qty

Storage Temp. (C)

4390303

BigDyeTM v3.0 Terminator Cycle

Sequencing Standard

Name

20

4390236

BigDyeTM v3.0 Terminator Cycle

Sequencing Ready Reaction

20

Note This is a sample kit used

by Field Application Specialists
for customer training.

2-6 Site Preparation

4316357

3100 POP-6 polymer

4311320

Hi-Di formamide

20

402824

10X Buffer with EDTA

4315930

Capillary Array 16 x 50 cm

4326477

Kit, BigDye v3.0 Software

ABI PRISM 3100 DNA v3.0 Seq + GS Chemistry Install Kit (P/N 4315985)
Part Number

Name

Qty

Storage Temp. (C)

4316100

3100 GeneScan Matrix Standard

Set DS-30

4390303

BigDye Terminator v3.0

Sequencing Standard

20

4390236

BigDye Terminator v3.0 Cycle

Sequencing Ready Reaction

20

Note This is a sample kit used

by Field Application Specialists
for customer training.
4316144

3100 GeneScan Installation

Standard

4316355

3100 POP-4 polymer

4316357

3100 POP-6 polymer

4311320

Hi-Di formamide

20

402824

10X Buffer with EDTA

4315930

Capillary Array 16 x 50 cm

4315931

Capillary Array 16 x 36 cm

4326477

Kit, BigDye v3.0 Software

Site Preparation 2-7

Storing the Reagents for the ABI PRISM 3100-Avant Genetic Analyzer
Chemistry Unpack the ABI PRISM 3100-Avant Genetic Analyzer Chemistry Installation Kit upon
Installation Kit receipt, and store the chemicals and reagents as indicated in the table below. The
chemicals in this kit are completely consumed during the installation and initial testing
of the instrument. There are three types of chemistry installation kit. The kit that you
receive depends on whether you ordered the Sequencing Software Module, the
GeneScan Software Module, or the Combination Software Module.
ABI PRISM 3100-Avant GeneScan Install Kit (P/N 4333731)
Part Number

Name

Qty

Storage Temp. (C)

4316100

3100 GeneScan Matrix Standard

Set DS-30

4316144

3100 GeneScan Installation

Standard

4316355

3100 POP-4 polymer

4311320

Hi-Di formamide

20

402824

10X Buffer with EDTA

4333464

Capillary Array 4 x 36 cm

ABI PRISM 3100-Avant DNA v3.0 Sequencing Chemistry Install Kit (P/N 4333732)
Part Number

Qty

Storage Temp. (C)

4390303

BigDye v3.0 Terminator Cycle

Sequencing Standard

Name

20

4390236

BigDye v3.0 Terminator Cycle

Sequencing Ready Reaction

20

Note This is a sample kit used

by Field Application Specialists
for customer training.

2-8 Site Preparation

4390436

BigDye v3.0 Matrix Standard

3100

4316357

3100 POP-6 polymer

4311320

Hi-Di formamide

20

402824

10X Buffer with EDTA

4315930

Capillary Array 16 x 50 cm

4326477

Kit, BigDye v3.0 Software

4333466

Capillary Array 4 x 50 cm

ABI PRISM 3100-Avant DNA v3.0 Seq + GS Chemistry Install Kit (P/N 4333733)
Part Number

Name

Qty

Storage Temp. (C)

4316100

3100 GeneScan Matrix Standard

Set DS-30

4390303

BigDye Terminator v3.0

Sequencing Standard

20

4390436

BigDye Matrix Standard

4390236

BigDye Terminator v3.0 Cycle

Sequencing Ready Reaction

20

This is a sample kit used by Field

Application Specialists for
customer training.
4316144

3100 GeneScan Installation

Standard

4316355

3100 POP-4 polymer

4316357

3100 POP-6 polymer

4311320

Hi-Di formamide

20

402824

10X Buffer with EDTA

4333466

Capillary Array 4 x 50 cm

4333464

Capillary Array 4 x 36 cm

4326477

Kit, BigDye v3.0 Software

Computer The computer that accompanies this instrument is a process with the Microsoft
Specifications Windows NT 4.0 operating system. Do not use this computer for purposes other than
operating this instrument.
IMPORTANT Do not install optional or unnecessary software. Applied Biosystems reserves
the right to remove all software not supplied by Applied Biosystems in order to determine
system functionality.

Networking Applied Biosystems will supply and install, but not support or connect, a network card
or install network connections. Customers must make arrangements with their own
network administration to have this done concurrent with or immediately after
installation of the instrument.

Site Preparation 2-9

Laboratory Safety Requirements

On-Site We request that a representative from your laboratory be in the vicinity and available
Representative to the Applied Biosystems engineer at all times while the engineer is at your facility.
This person should be familiar with laboratory safety procedures and know the
location of all the safety equipment.

Required Safety Your laboratory has specific safety practices and policies designed to protect
Equipment laboratory personnel from potential hazards that are present. We expect that all
applicable safety-related procedures will be followed at all times.
The following safety equipment must be available:

2-10 Site Preparation

Fire extinguisher

It is your responsibility to provide appropriate fire extinguishers for use on or

near Applied Biosystems equipment.

The types and sizes of fire extinguishers shall be suitable for use on electrical
and chemical fires as specified in currently prevailing codes, regulations
and/or standards, and with the approval of the Fire Marshall or other authority
having jurisdiction.

The installation of appropriate fire extinguishers shall be in addition to other

fire protection systems and not as a substitute or alternative to them.

Eyewash
Safety shower
Eye and hand protection
Adequate ventilation
First-aid equipment
Spill clean-up equipment
Protection from any sources of hazardous chemicals, radiation (lasers,
radioisotopes, contaminated equipment, radioactive wastes, etc.) and potentially
infectious biological material that may be present in the area where Applied
Biosystems engineer(s) will be working

Laboratory Space Required

Dimensions and The ABI PRISM 3100 Genetic Analyzer and computer have the following dimensions:
Weight
Component

Width

Depth

Height

Weight

3100 Genetic
Analyzer, crated

94 cm
(37 in.)

70 cm
(27.5 in.)

105 cm
(41.5 in.)

142 kg
(313 lb)

3100 Genetic
Analyzer, uncrated

74 cm
(29.2 in.)

54.8 cm
(21.6 in.)

81 cm
(32 in.)

120 kg
(265 lb)

3100 Genetic
Analyzer with all
required clearances

148.6 cm
(58.5 in.)

67 cm
(26.4 in.)

111 cm
(44 in.)

Computer CPU

21 cm
(8.3 in.)

44 cm
(17.4 in.)

44 cm
(17.4 in.)

10 kg
(25 lb)

Monitor

42 cm
(16.6 in.)

53 cm
(20.9 in.)

44 cm
(17.4 in.)

18 kg
(40 lb)

Instrument Location Plan to provide sufficient laboratory space for the instrument system as well as the
following accommodations:

15 cm (6 in.) of clearance at the rear of the instrument for adequate ventilation.

A laboratory bench of correct dimensions and weight tolerance to accommodate

the system.

The computer must be no more than 3.7 m (12 ft) from the instrument.

Access to all four sides of the instrument for servicing. Do not block access to the
rear of the instrument. One way to provide access to the rear of the instrument is
to put the instrument on a table with wheels.

The computer monitor and keyboard situated to allow for proper ergonomics
during use. The following guidelines are recommended:

Bench space (or pull-out tray) provided so that the keyboard is positioned
directly in line with the monitor

Open space under the keyboard so that the operator can sit comfortably,
directly facing the monitor

Work space provided next to the monitor

Helping the At least three people will be needed to help the service engineer place the instrument
Engineer Move the onto the laboratory bench. Do not unpack or move the instrument before the service
Instrument engineer arrives to install it.
! WARNING PHYSICAL INJURY HAZARD. Do not attempt to lift the instrument or any
other heavy objects unless you have received related training. Incorrect lifting can cause painful
and sometimes permanent back injury. Use proper lifting techniques when lifting or moving the
instrument. Two or three people are required to lift the instrument, depending upon instrument
weight.

Site Preparation 2-11

Typical Laboratory A typical laboratory layout is shown below.

Layout

2-12 Site Preparation

Laboratory Ventilation Requirements

Heat Production The maximum thermal output of the instrument is 6800 Btu/h (~2000 W). Consult your
facilities department regarding ventilation requirements for this level of heat output in
your particular laboratory.

System Vent There are two heat-venting fans at the back of the instrument: the chassis fan and the
laser fan.
If the laboratory room ventilation will accommodate the heat produced by this
instrument, the instrument can be vented to room air.
If the room temperature will rise above optimal operating levels because of the heat
produced by this instrument, install flexible duct (elephant trunk) from the instrument
to the laboratory ventilation system (fume hood or duct). A connector with an outer
diameter of 12.7 cm (5.0 in.) is provided to connect the laser vent to a flexible duct.

Connecting the Follow these guidelines if connecting a flexible duct from the instrument laser vent to
Flexible Duct your laboratory ventilation system.

Use the shortest possible length and straightest possible run of flexible duct.

Keep the flexible duct away from sources of potential damage, such as from
contact with heat or flame, or rubbing against other objects.

Make sure the flexible duct does not have low points that can trap residue or
condensation.

Laboratory Environmental Requirements

Altitude This instrument is for indoor use only and for altitudes not exceeding 2000 m (6500 ft)
above sea level.

Temperature and The laboratory temperature should be maintained between 15 to 30 C (59 to 86 F),
Humidity and remain staable at the set temperature (within +/- 2 C). The instrument can
tolerate between 20 to 80% relative humidity. Avoid placing the instrument adjacent to
heaters or cooling ducts, or in direct sunlight.

Pollution The installation category (overvoltage category) for this instrument is II, and it is
classified as portable equipment. The instrument has a pollution degree rating of 2
and may be installed in an environment that has nonconductive pollutants only.

Emission/Immunity For our European customers, any product marked with the CE label meets the
Statement European EMC Directive 89/336/EEC and the Low Voltage Directive 72/23/EEC. This
product meets Class B emission limits.

Site Preparation 2-13

Electrical Requirements
Power The following table specifies the electrical operating range for various parts of the
world:
IMPORTANT In Japan, the unit must have a dedicated 200-volt outlet. The unit will not
operate properly with a 100-volt outlet.
Location

Voltage (VAC)

Japan
USA/Canada
Europe

Frequency

Maximum Current (A)

50/60 Hz 1%
200 to 229
230 to 240

Australia

60 Hz 1%
50 Hz 1%

11.2

50 Hz 1%

Power Line The electrical receptacle must have a dedicated 2.5 kVA power line and ground.
Electrical Outlets The instrument requires a 30 amp receptacle to match one of the two power cord
configurations that ship with the system. See the Power Cords section below.
The electrical receptacle must be located within 3 m (10 ft) of the back of the
instrument. Do not use extension cords.
IMPORTANT Despite the apparent dissimilarity between the capacity requirements for the
power line (2.5 KVA) and the receptacle (30 A), these are the system requirements.

Power Rating This instrument is rated for a maximum input of 2500 VA.
Power Cords In the USA, Canada, and Japan, the instrument is supplied with a detachable cord
equipped with a Nema L6-30P plug.
In Europe and Australia, the instrument is supplied with a detachable electrical cord
equipped with an IEC 309 plug.
The computer, CPU and monitor, can be plugged into any standard electrical
receptacles after they have been configured for the proper voltage.

Grounding Certain types of electrical noise are greatly exaggerated by poor or improper electrical
ground connections. To prevent these problems, it is very important to have a
dedicated ground line between the instrument and the buildings main electrical
service.

Power Line In areas where the supplied power is subject to voltage fluctuations that exceed 10%
Regulator of the nominal voltage (see the section, Power, above) a power line regulator may be
required. High or low voltages can have adverse effects on the electronic components
of the instrument.

2-14 Site Preparation

Voltage Spikes Short-duration, high-voltage spikes often cause random failures in

microprocessor-controlled instrumentation. These spikes can be caused by other
devices using the same power source (refrigerators, air conditioners, and centrifuges)
or by outside influences such as lightning. A dedicated line and ground between the
instrument and the buildings main electrical service will prevent such problems.
If your environment contains devices that are electrically noisy, or you are in an area
with frequent electrical storms, a line conditioner with a recommended capacity of
1.0 kVA will enhance the reliability of your system.

Power Outages The instrument has not been designed to continue with a run after power failure. A run
that has been interrupted by power failure must be restarted. For this reason, we
recommend that you protect against power outage by installing an uninterruptible
power supply (UPS) with an output capacity of 2.5 kVA (30 minutes at 2.4 kVA after
full charge). We recommend the UPS from Franek Company. The order number for
the appropriate UPS is FT1-B3100-DA. Franeks phone number is 1-800-326-6480,
their fax number is 714-554-6957, their website is www.Franek-tech.com, and their
e:mail address is sales@Franek.tech.com.

Electric Shock ! WARNING ELECTRICAL SHOCK HAZARD. Severe electrical shock, which could cause
Warning physical injury or death, can result from working on an instrument when the high-voltage power
supply is operating. To avoid electrical shock, disconnect the power supply to the instrument,
unplug the power cord, and wait at least 1 minute before working on the instrument.

Site Preparation 2-15

Chemical Safety

Overview
In This Chapter This chapter contains general information about handling hazardous chemicals and
waste. It also contains information for the ABI PRISM 3100 Genetic Analyzer and
ABI PRISM 3100-Avant Genetic Analyzer about:

Material safety data sheets (MSDSs)

Hazardous waste produced during use of the instrument

Applied Biosystems assumes that all operations in your laboratory will be conducted
in accordance with safety practices detailed in the MSDSs for the chemicals used in
your laboratory, and with all local, state/provincial, or national regulations.

Chemical Safety 3-1

Material Safety Data Sheets (MSDSs) Overview

About MSDSs Some of the chemicals used with this instrument may be listed as hazardous by their
manufacturer. When hazards exist, warnings are prominently displayed on the labels
of all chemicals.
MSDSs are supplied by the chemical manufacturer and provide information about:

Physical characteristics
Safety precautions
Health hazards
First aid
Spill clean-up
Disposal procedures

! WARNING CHEMICAL HAZARD. Be sure to familiarize yourself with the MSDSs before
using reagents or solvents.

Explanations of acronyms and abbreviations used in MSDSs can be found in

Appendix A of this guide.

Updating MSDSs Chemical manufacturers supply a current MSDS before or with shipments of
hazardous chemicals to new customers and with the first shipment of a hazardous
chemical after an MSDS update.
Replace the MSDSs in your files regularly so that the safety information is current.

Ordering MSDSs You can order free additional copies of MSDSs for chemicals manufactured or
from distributed by Applied Biosystems.
Applied Biosystems
Ordering MSDSs Applied Biosystems does not furnish MSDSs for chemicals used on this instrument
from Other that are not manufactured or distributed by Applied Biosystems. Contact the
Manufacturers manufacturer(s) of those chemicals to obtain additional MSDSs.

3-2 Chemical Safety

Hazardous Chemicals
Overview This instrument may use chemicals that are hazardous.
! WARNING CHEMICAL HAZARD. Hazardous chemicals used with this instrument can
cause injury, illness, or death. Handle all chemicals as potentially hazardous.

Chemicals are classified as hazardous when they are physically hazardous or if they
can cause acute or chronic health hazards upon exposure.

Physically hazardous chemicals are materials that are flammable, combustible,

under compression (gases), explosive, oxidative, organically peroxidic,
pyrophoric, reactive or unstable, or water reactive.

Chemicals that may cause health hazards include carcinogens; materials that are
toxic or highly toxic; reproductive toxins; irritants; corrosives; sensitizers; materials
that are toxic to the liver, kidney or blood-forming (hematopoietic) system; and
agents that damage the lungs, skin, eyes, or mucous membranes.

Handling Hazardous When handling hazardous chemicals it is important to:

Chemicals
Read and understand all applicable MSDSs before handling hazardous
chemicals.

Always wear gloves, safety glasses, and protective clothing when handling
chemicals.

Always provide adequate ventilation when handling chemicals. Some chemicals

require handling only in a properly functioning fume hood.

Provide secondary containment for all reagent bottles.

Do not store chemicals in direct sunlight or heat (on or off the instrument).

Chemical Safety 3-3

Hazardous Waste
Overview This instrument may generate hazardous waste.
! WARNING CHEMICAL WASTE HAZARD. Wastes produced by Applied Biosystems
instruments are potentially hazardous, and can cause injury, illness, or death.

Read and understand the material safety data sheets (MSDSs) provided by the
manufacturers of the chemicals in the waste container before you store, handle, or dispose
of chemical waste.

Handle chemical wastes in a fume hood.

Dispose of the contents of the waste reservoir in accordance with good laboratory practices
and local, state/provincial, or national environmental and health regulations.

Venting hazardous waste may require local, state/provincial, or national air permits.
Minimize contact with and inhalation of chemical waste. Wear appropriate personal
protective equipment when handling chemicals (e.g., safety glasses, gloves, or clothing).

Instrument Waste The composition of the chemical waste may vary depending upon the protocols used,
System the number of samples, the volumes specified, and the reagents included in the
protocols.

Handling Chemical When handling chemical waste we strongly recommend that you:
Waste
Read the waste profile(s) in this chapter before handling or disposing of
hazardous waste.

3-4 Chemical Safety

Read all applicable MSDSs before handling or disposing of hazardous waste.

Always wear chemical-resistant gloves, safety glasses, and protective clothing

when handling hazardous waste material.

Dispose of hazardous waste in accordance with all local, state/provincial, or

national regulations.

Always handle hazardous materials beneath a fume hood that is connected in

accordance with all installation requirements.

Storing Hazardous ! WARNING CHEMICAL STORAGE HAZARD. Never collect or store waste in a glass
Waste container because of the risk of breaking or shattering. Reagent and waste bottles can crack

and leak. Each waste bottle should be secured in a low-density polyethylene safety container
with the cover fastened and the handles locked in the upright position. Wear appropriate
eyewear, clothing, and gloves when handling reagent and waste bottles.

The following are guidelines for storing hazardous waste:

Always use secondary containment when storing chemical waste.

Store waste for only short periods of time.
Store only small amounts of waste in the laboratory.
Store waste away from direct sunlight or sources of heat (on or off the instrument).

Disposing of As the generator of potentially hazardous waste, it is your responsibility to:

Hazardous Waste
Characterize the waste generated with your applications.

Ensure the health and safety of all personnel in your laboratory.

Ensure that instrument waste is stored, transferred, transported, and disposed of
according to all local, state/provincial, or national regulations.

Note Radioactive or biohazardous materials may require special handling, and disposal
limitations may apply.

Chemical Safety 3-5

Instrument Safety

Overview
In This Chapter This chapter provides you with the safety information you need to prepare your
laboratory and personnel for the installation and use of the ABI PRISM 3100 Genetic
Analyzer and the ABI PRISM 3100-Avant Genetic Analyzer. The safety labels and
safety alert symbols that may be found on this instrument are provided in several
languages. The input and output connections (such as for power and computer
cables) on this instrument are also provided.

Safe Operation This manual provides only site preparation information. Before operating this
instrument, read the information in the ABI PRISM 3100 Genetic Analyzer Users
Manual (P/N 4315834) concerning hazards and potential hazards. Make sure that
anyone involved with the operation of the instrument is instructed both in general
safety practices for laboratories and in specific safety practices for the instrument.

Routine Maintain the instrument in good working order. If the instrument has been subjected to
Maintenance for adverse environmental conditions (such as fire, flood, or earthquake), an
Safe Operation Applied Biosystems service representative should inspect the instrument.
We recommend that an Applied Biosystems service representative check the
instrument yearly to verify that:

The safety interlocks protecting the user from various hazards are working
properly.

The protective housing is functional. Loose or distorted panels will not protect the
user or the equipment.

Airflow is not hindered in any way.

Instrument Safety 4-1

Instrument Labeling
Safety Labels Safety labels are located on the instrument. Each label consists of a Signal Word
panel and a Message panel. A Safety Alert symbol indicates a potential personal
safety hazard. If multiple hazards exist, the signal word corresponding to the greatest
hazard is used.

Signal Words

4-2 Instrument Safety

! CAUTION indicates a potentially hazardous situation that could result in minor

or moderate injury to the user or damage to the instrument.

! WARNING indicates a potentially hazardous situation that could result in

death or serious injury.

! DANGER indicates an imminently hazardous situation that, if not avoided, will

result in death or serious injury (most extreme).

Labels that May Be The following Danger, Caution, and Warning labels, listed in English and French, may
Found on the be found on your instrument:
Instrument
English

French

CAUTION: Hazardous chemicals. Read the

Material Safety Data Sheets (MSDSs)
before handling.

ATTENTION: Produits chimiques

dangeureux. Lire les fiches techniques de
sret de matriels avant la manipulation
des produits.

CAUTION: Hazardous waste. Read the

Waste Profile before handling or disposal.

ATTENTION: Dchets dangereux. Lire les

renseignements sur les dchets avant de les
manipuler ou de les liminer.

WARNING: Risk of electric shock.

Disconnect power cord from supply before
replacing fuses or removing power supply
module from instrument.

AVERTISSEMENT: Risque de choc

lectrique. Dbrancher le cordon
dalimentation avant de remplacer les
fusibles ou de retirer le bloc dalimentation
de linstrument.

WARNING: For continued protection against

risk of fire, replace only with Listed and
Certified fuse of the specified type and
ratings.

AVERTISSEMENT: Pour une protection

continue contre les risques dincendie,
utiliser uniquement des fusibles agr et
certifis du type et du courant nominal
spcifis.

WARNING: Hot lamp.

AVERTISSEMENT: Lampe brulante.

WARNING: Hot. Replace lamp with a

Applied Biosystems lamp.

AVERTISSEMENT: Composants brlants.

Remplacer la lampe par une lampe Applied
Biosystems.

WARNING: Disconnect supply cord before

opening. Grounding circuit continuity is vital
for safe operation of equipment. Never
operate equipment with grounding conductor
disconnected.

AVERTISSEMENT: Dbrancher le cordon

dalimentation avant douvrir. La continuit
du circuit de masse est essentiel la
scurit du fonctionnement de lappareil. Ne
jamais utiliser lappareil avec la prise de
terre dbranche.

WARNING: For protection against fire

hazard, replace only same type and rating of
fuse.

AVERTISSEMENT: Pour assurer la

protection contre les risques dincendie,
remplacer uniquement par un fusible de
mme type et de mme courant nominal.

CAUTION: Hot.

ATTENTION: Surface brlante.

DANGER: High voltage.

DANGER: Haut tension.

WARNING: To reduce the chance of

electrical shock, do not remove covers that
require tool access. No user serviceable
parts are inside. Refer servicing to
Applied Biosystems qualified service
personnel.

AVERTISSEMENT: Pour viter les riques

dlectrocution, ne pas retirer les capots en
vue dune intervention laide doutils.
Linstrument ne contient aucune pice
rparable par lutilisateur. Toute intervention
doit tre effectue par le personnel de
service qualifi de Applied Biosystems.

DANGER: Laser radiation when open and

interlock defeated. Avoid direct exposure to
beam.

DANGER: Rayonnement laser en cas

douverture et dune neutralisation des
dispositifs de scurit. Eviter toute
exposition directe avec le faisceau.

CAUTION: Moving parts.

ATTENTION: Parties mobiles.

Instrument Safety 4-3

Safety Alert Symbols

Electrical Symbols The following chart is an illustrated glossary of all electrical symbols that are used on
Applied Biosystems instruments. Whenever such symbols appear on instruments,
please observe appropriate safety procedures.
This symbol indicates the On position of the main power switch.

This symbol indicates the Off position of the main power switch.

This symbol indicates the On/Off position of a push-push main power switch.

This symbol indicates that a terminal may be connected to another

instruments signal ground reference. This is not a protected ground
terminal.
This symbol indicates that this is a protective grounding terminal that must
be connected to earth ground before any other electrical connections are
made to the instrument.
A terminal marked with this symbol either receives or delivers alternating
current or voltage.

A terminal marked with this symbol can receive or supply an alternating and
a direct current or voltage.

This symbol indicates the presence of high voltage and warns the user to
proceed with caution.

This symbol alerts you to consult the manual for further information and to
proceed with caution.

Nonelectrical The following is an illustrated glossary of all nonelectrical safety alert symbols found
Symbols on Applied Biosystems instruments.
This symbol illustrates a heater hazard. Proceed with caution when working
around these areas to avoid being burned by hot components.

This symbol indicates that a laser is present inside the instrument.

4-4 Instrument Safety

Safety Alert Symbols (French)

Symboles des alertes de scurit

Symboles lectriques Le tableau suivant donne la signification de tous les symboles lectriques qui figurent
sur les appareils Applied Biosystems. En prsence de lun de ces symboles, il est
impratif de se conformer aux consignes de scurit appropries.
Position MARCHE de linterrupteur dalimentation principal.

Position ARRT de linterrupteur dalimentation principal.

Positions MARCHE-ARRT de linterrupteur dalimentation principal

bouton poussoir.

Borne pouvant tre relie la mise la terre dun autre appareil. Ce nest
pas une borne de mise la terre protge.

Borne de mise la terre de protection devant tre relie la terre avant

deffectuer tout autre raccordement lectrique lappareil.

Borne recevant ou fournissant une tension ou un courant de type alternatif.

Borne pouvant recevoir ou fournir une tension ou un courant de types

alternatif et continu.

Indique la prsence dune haute tension et avertit lutilisateur de procder

avec prcaution.

Avertit lutilisateur de la ncessit de consulter le manuel pour obtenir

davantage dinformations et de procder avec prcaution.

Symboles non Le tableau suivant donne la signification des symboles dalertes de scurit non
lectriques lectriques qui figurent sur les appareils Applied Biosystems.
Danger associ la prsence dun appareil de chauffage. Procder avec
prcaution pour viter de se brler au contact de pices ou dlments
chauds.
Indique que lappareil renferme un laser.

Instrument Safety 4-5

Safety Alert Symbols (German)

Sicherheitswarnsymbole
Elektrische Symbole Die folgende Tabelle enthlt Beschreibungen aller auf den Gerten von
Applied Biosystems verwendeten elektrischen Symbole. Wenn diese Symbole auf
den Gerten erscheinen, beachten Sie bitte die entsprechenden
Sicherheitsmanahmen.
Dieses Symbol zeigt die EIN-Position des Hauptnetzschalters an.

Dieses Symbol zeigt die AUS-Position des Hauptnetzschalters an.

Dieses Symbol zeigt die EIN/AUS-Position eines einrastenden

Hauptnetzdruckschalters an.

Dieses Symbol zeigt an, da ein Anschlu an die Betriebserde eines

anderen Gerts angeschlossen werden kann. Dies ist keine geschtzte
Erdklemme.
Dieses Symbol zeigt eine geschtzte Erdklemme an, die geerdet werden
mu, bevor andere elektrische Anschlsse zum Gert hergestellt werden.

Ein mit diesem Symbol gekennzeichneter Anschlu kann Wechselstrom

oder -spannung erhalten oder abgeben.

Ein mit diesem Symbol gekennzeichneter Anschlu kann Wechselstrom

oder -spannung und Gleichstrom oder -spannung erhalten oder abgeben.

Dieses Symbol zeigt das Vorliegen von Hochspannung an und warnt den
Anwender, vorsichtig vorzugehen.

Dieses Symbol fordert Sie auf, das Handbuch zwecks nherer Informationen
zu konsultieren und vorsichtig vorzugehen.

Nicht-elektrische Die folgende Tabelle enthlt Beschreibungen aller auf den Gerten von
Symbole Applied Biosystems verwendeten nicht-elektrischen Symbole.
Dieses Symbol zeigt eine Gefahr durch die Heizung an. Gehen Sie
vorsichtig vor, wenn Sie in der Nhe dieser Bereiche arbeiten, damit Sie sich
nicht an heien Komponenten verbrennen.
Dieses Symbol zeigt das Vorliegen eines Lasers im Innern des Gerts an.

4-6 Instrument Safety

Safety Alert Symbols (Italian)

Simboli degli allarmi di sicurezza

Simboli elettrici La tabella seguente un glossario illustrato di tutti i simboli elettrici utilizzati su
strumenti Applied Biosystems. Ogni volta che tali simboli compaiono sugli strumenti,
rispettare le procedure di sicurezza appropriate.
Questo simbolo indica la posizione ON dellinterruttore di alimentazione
generale.

Questo simbolo indica la posizione OFF dellinterruttore di alimentazione

generale.

Questo simbolo indica la posizione ON/OFF di un interruttore di

alimentazione generale a pulsante.

Questo simbolo indica che un terminale pu essere collegato al riferimento

di terra del segnale di un altro strumento. Non un terminale di terra
protetto.
Questo simbolo indica un terminale protettivo di messa a terra che deve
essere collegato a terra prima di realizzare qualsiasi altro collegamento
elettrico allo strumento.
Un terminale contrassegnato con questo simbolo riceve o fornisce tensione
o corrente alternata.

Un terminale contrassegnato con questo simbolo pu ricevere of fornire

tensione o corrente alternata e continua.

Questo simbolo indica la presenza di alta tensione e invita lutente a

procedere con cautela.

Questo simbolo invita lutente a consultare il manuale per ulteriori

informazioni e a procedere con cautela.

Simboli non elettrici Segue un glossario illustrato dei simboli degli allarmi di sicurezza non elettrici trovati
su strumenti Applied Biosystems.
Questo simbolo illustra un rischio da alte temperature. Procedere con
cautela quando si lavora in queste aree per evitare ustioni causate di
componenti a temperature elevate.
Questo simbolo indica la presenza di laser nello strumento.

Instrument Safety 4-7

Saftey Alert Symbols (Portuguese)

Smbolos de alerta de segurana
Smbolos eltricos A tabela a seguir constitui um glossrio ilustrado de todos os smbolos eltricos
usados nos instrumentos Applied Biosystems. Sempre que um desses smbolos
aparecer num instrumento, siga os procedimentos adequados de segurana.
Este smbolo indica que o interruptor de energia eltrica est na posio
ligado.

Este smbolo indica que o interruptor de energia eltrica est na posio

desligado.

Este smbolo indica a posio ligado/desligado de um interruptor principal

de energia eltrica do tipo boto de presso.

Este smbolo indica que um terminal pode estar conectado a uma referncia
de aterramento de sinal de um outro instrumento. Este no um terminal
terra protegido.
Este smbolo indica que este um terminal de aterramento de proteo, que
deve ser ligado terra antes de se fazer qualquer outra ligao eltrica ao
instrumento.
Um terminal marcado com este smbolo recebe ou transmite tenso ou
corrente alternada.

Um terminal marcado com este smbolo recebe ou fornece tenso ou

corrente alternada ou contnua.

Este smbolo indica a presena de alta tenso e avisa o usurio para

proceder com cuidado.

Este smbolo serve como alerta, para que se consulte o manual a fim de se
obter mais informaes e que se proceda com cuidado.

Smbolos A seguir, apresentamos um glossrio ilustrado de todos os smbolos de alerta de

no-eltricos segurana no relacionados electricidade encontrados nos instrumentos
Applied Biosystems.
Este smbolo representa um perigo devido a aquecedor no local. Proceda
com cuidado ao trabalhar em reas prximas a aquecedores, para evitar
queimaduras devidas ao contato com componentes quentes.
Este smbolo indica que h um laser dentro do instrumento.

4-8 Instrument Safety

Safety Alert Symbols (Spanish)

Smbolos de alerta de seguridad
Smbolos elctricos En la siguiente tabla se muestra un glosario ilustrado de todos los smbolos elctricos
que se utilizan en los instrumentos de Applied Biosystems. Cuando tales smbolos
figuran en los instrumentos, lleve a cabo los procedimientos de seguridad apropiados.
Este smbolo indica la posicin de encendido del interruptor principal.

Este smbolo indica la posicin de apagado del interruptor principal.

Este smbolo indica la posicin de encendido/apagado de un interruptor

principal de presin.

Este smbolo indica que existe la posibilidad de conectar esta terminal a la

toma de tierra de referencia de otro instrumento. sta no es una toma de
tierra protegida.
Este smbolo indica que la toma de tierra protegida debe ser conectada a
tierra antes de realizar cualquier otro tipo de conexin elctrica al
instrumento.
Una terminal marcada con este smbolo recibe o suministra corriente o
tensin alterna.

Una terminal marcada con este smbolo puede recibir o suministrar corriente
o tensin alterna y continua.

Este smbolo indica la presencia de alta tensin y advierte al usuario que

proceda con precaucin.

Este smbolo indica que consulte el manual para obtener ms informacin y

que proceda con precaucin.

Smbolos no A continuacin se presenta un glosario ilustrado de todos los smbolos de seguridad y

elctricos alerta no elctricos que aparecen en los instrumentos de Applied Biosystems.
Este smbolo indica peligro de altas temperaturas. Proceda con cautela
cuando trabaje cerca de estas zonas para evitar quemarse con
componentes calientes.
Este smbolo indica que hay un lser dentro del instrumento.

Instrument Safety 4-9

Safety Alert Symbols (Chinese)

4-10 Instrument Safety

Safety Alert Symbols (Japanese)

Instrument Safety 4-11

Safety Alert Symbols (Korean)

4-12 Instrument Safety

Safety Alert Symbols (Thai)

Instrument Safety 4-13

Input/Output Connection
Ethernet Connection The location of the ethernet cable that is used to communicate with the computer is
shown in the figure below.

Ethernet cable
connector

4-14 Instrument Safety

Laser Safety
Laser Type and This instrument uses an argon laser.
Class
Laser class
Under normal operating
conditions

Class I

During servicing (or when

safety features are defeated)

Class IIIb, extremely

hazardous

Laser Radiation Laser radiation has two hazardous properties that make it different from lamp light or
sunlight. These properties are intensity and directionality.
Intensity
Some lasers are so intense that exposures to the eye of less than one-thousandth of a
second can cause permanent retinal burns and blind spots. Lasers lose very little
energy over small distances, and reflected laser light can be as hazardous as the
original beam.
Directionality
Laser radiation is directional and can radiate for a long distance with very little
scattering or dispersion.

Laser Safety ! WARNING LASER HAZARD. Exposure to direct or reflected laser light can burn the retina
Requirements and leave permanent blind spots. Never look directly into the laser beam. Do not defeat the
laser interlocks on the doors and/or panels. Do not remove the protective housing around the
laser assembly, or any other device designed to block the laser beam. Do not remove the laser
safety labels from the instrument.

Observe the following laser safety requirements:

Do not attempt to defeat the laser safety interlock switches on the doors and/or
panels. These switches turn the laser off when the doors or panels are open,
protecting the user from exposure to the laser beam.

The outside cabinet panels and doors of this product must always be in place
during operation.

Do not attempt to remove the protective housing around the laser assembly.

There should be no detectable radiation when the instrument is installed properly.

The installation and servicing of the laser must be performed only by

Applied Biosystems service personnel.

Do not remove or manipulate any beam-blocking device (for example, a

beam-stop bar), which may be present on the instrument.
Laser and power supplies of this instrument contain electrical circuits operating at
high voltages that can cause electrical shock.

Instrument Safety 4-15

Acronyms and
Abbreviations

Acronyms and Abbreviations Used in MSDSs

Introduction Material Safety Data Sheets (MSDSs) use acronyms and abbreviations for certain
organizations, government regulations, common scientific terminology, units of
measurement, and chemicals. This appendix is provided to help you understand
these references while reading the MSDSs for the chemicals used in your laboratory.

Organizations, The following table lists acronyms and abbreviations for organizations, government
Regulations, and regulations, and scientific terminology
Scientific Organizations, Regulations, and Scientific Terminology
Terminology
Term

Explanation

ACGIH

American Conference of Governmental Industrial Hygienists

CAS#

Chemical Abstract Service Reference Number for Specific Pure Chemical

cc

Closed cup testing of flash point

CFR

Code of Federal Regulations. Regulations published by the United States

Government

CERCLA

Comprehensive Environmental Response, Compensation and Liability Act

(Superfund) is a federal law administered by EPA

DFG MAK

Federal Republic of Germanys Maximum Contamination Value in the

workplace (similar to PEL in the USA)

DOT

United States Department of Transportation, regulates transportation of

hazardous material (USA)

EPA

United States Environmental Protection Agency, regulates use, disposal, or

emission of hazardous material (USA)

IDLH

Immediate Danger to Life and Health

LCLO

Lowest published lethal concentration

LC50

Lethal concentration in air that kills 50% of a specified population

LD50

Lethal dose that kills 50% of a specified population

LEL

Lower Explosion Limit

MSHA

Mine Safety and Health Administration, recommends respirators

NFPA

National Fire Protection Association, publishes recommended regulations for

local or state governments in the United States (Hazardous rating system
developed by this Association)

NIOSH

National Institute of Occupational Safety and Health (USA), recommends

exposure levels and respirators

Acronyms and Abbreviations A-1

Organizations, Regulations, and Scientific Terminology

(continued)

Term

Explanation

oc

Open cup testing for flash point

OSHA

Occupational Safety and Health Administration (USA), sets chemical exposure

levels.

PEL

Permissible Exposure Limit. The federal OSHA limit, usually expressed as time
weighted average (TWA) for an 8-hour work shift.

PPM

Parts Per Million

Prop 65

A California Law requiring warnings for chemicals that are known to the state to
be carcinogenic or cause reproductive harm.

RCRA

Resource Conservation and Recovery Act

RTECS

Registry of Toxic Effects of Chemical Substances

SARA

Superfund Amendments and Reauthorization Act, a federal act administered

by EPA

SCBA

Self-Contained Breathing Apparatus

STCC

Standard Transportation Commodity Code

STEL

Short Term Exposure Level, published by ACGIH

TCLO

Lowest published toxic concentration

TLV

Threshold Limit Value. The ACGIH-recommended TWA, usually for an 8-hour

work shift

TWA

Time Weighted Average

UEL

Upper Explosive Limit

u or U

Unknown

UN

United Nations. This designation identifies hazardous chemicals in the process

of worldwide transportation.

Units of Measure The following table lists abbreviations for common units of measure
Units of Measure
Abbrev.

Unit of Measure

number

degrees Celsius

degrees Fahrenheit

microliter

micron

mol

micromole

AUFS

absorbency units full-scale

Btu

British thermal unit

ft

foot

gal.

gallon

hour

i.d.

inside diameter

in.

inch

kVA

kilovoltampere

A-2 Acronyms and Abbreviations

Units of Measure

(continued)

Abbrev.

Unit of Measure

liter

meter

mg

milligram

mL

milliliter

mm

millimeter

o.d.

outside diameter

P/N

part number

psi

pounds per square inch

sec

second

volt

VA

voltampere

VAC

volts, alternating current

watt

Chemicals The following table lists abbreviations for common chemicals

Chemicals
Abbrev.

Definition

adenine

AA

amino acid

1Ac

acetyl

Acl

acetylimidazole

Acm

acetamidomethyl

Ac2O

acetic anhydride

ACN

acetonitrile

ACT

activator vessel

BHA resin

benzhydrylamine resin

t-Boc

tert-butyloxycarbonyl

Bzl

benzyl

Br-Z

2-bromobenzyloxcarbonyl

t-Bu

tert-butyl

cytosine

CHO

formyl

CH3Bzl

4-methylbenzyl

CH30Bzl

4-methoxybenzyl

Cl-Z

2-chlorobenzyloxycarbonyl

CPG

Controlled Pore Glass

DCA

dichloroacetic acid

DCC

dicyclohexylcarbodiimide

DCM

dichloromethane

DCU

dicyclohexylurea

Acronyms and Abbreviations A-3

Chemicals

(continued)

Abbrev.

Definition

DIEA

diisopropylethylamine

DMAP

4-dimethylaminopyridine

DMF

dimethylformamide

DMSO

dimethylsulfoxide

DNA

deoxyribonucleic acid

Dnp

2,4-dinitrophenyl

Et

ethyl

EtOH

ethanol

Fmoc

9-fluorenylmethyloxycarbonyl

guanine

HBTU

2-(1H-benzotriazol-1-yl)-1,1,3,3-tetramethyl-uronium hexafluorophosphate

HLP

high loaded polystyrene

HMP resin

p-hydroxymethylphenoxymethyl-polystyrene resin

HOAc

acetic acid

mBHA resin

4-methylbenzhydrylamine resin

MeOH

methanol

Mob

4-methoxybenzyl

Mtr

4-methoxy-2,3,6-trimethyl-benzene sulfonyl

Mts

mesitylene-2-sulfonyl

NMI

1-methylimidazole

NMP

N-methylpyrrolidone, N-methyl-2-pyrrolidone

OEt

ethyl ester

OMe

methyl ester

PAM resin

phenylacetamidomethyl resin

PEG

polyethylene glycol

RV

reaction vessel

SSPS

solid-phase peptide synthesis

thymine

TETD

tetraethylthiuram disulfide

TFA

trifluoroacetic acid

TFMSA

trifluoromethane sulfonic acid

THF

tetrahydrofuran

Tos

4-toluenesulfonyl (tosyl)

Tri

trityl

uracil

benzyloxcarbonyl

A-4 Acronyms and Abbreviations

Technical Support

Services & Support

Applied Biosystems To access the Applied Biosystems Web site, go to:
Web Site
http://www.appliedbiosystems.com

At the Applied Biosystems Web site, you can:

Search through frequently asked questions (FAQs)

Download PDF documents

Submit a question directly to Technical Support

Order Applied Biosystems user documents, MSDSs, certificates of analysis, and
other related documents
Obtain information about customer training
Download software updates and patches

In addition, the Applied Biosystems Web site provides a list of telephone and fax
numbers that can be used to contact Technical Support.

Technical Support B-1

Headquarters
850 Lincoln Centre Drive
Foster City, CA 94404 USA
Phone: +1 650.638.5800
Toll Free (In North America): +1 800.345.5224
Fax: +1 650.638.5884
Worldwide Sales and Support
Applied Biosystems vast distribution and
service network, composed of highly trained
support and applications personnel, reaches
into 150 countries on six continents. For sales
office locations and technical support, please
call our local office or refer to our web site at
www.appliedbiosystems.com.

www.appliedbiosystems.com

Applera Corporation is committed to providing

the worlds leading technology and information
for life scientists. Applera Corporation consists of
the Applied Biosystems and Celera Genomics
businesses.
Part Number 4315835 Rev. C
03/2002