Agilent GC Catalog PDF

Table of Contents
Sales, Service, and Support

Technical Support Information . . . . . . . . . . . . . . . .3 Training Information . . . . . . . . . . . . . . . . . . . . . . . .4
37
Services Information . . . . . . . . . . . . . . . . . . . . .45 Agilent Offices . . . . . . . . . . . . . . . . . . . . . . . . . .67
Featured Products General Chromatography

Vials, Caps and Septa . . . . . . . . . . . . . . . . . .1238 Syringes . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3946 Gas Management . . . . . . . . . . . . . . . . . . . . .4782 Hardware . . . . . . . . . . . . . . . . . . . . . . . . . . .8391
9 11109
Standards . . . . . . . . . . . . . . . . . . . . . . . . . .92101 Integrators, Integrator Supplies and Cables . .102106 Books, Learning Software and CDs . . . . . .107109
Sample Preparation
Solid Phase Extraction (SPE) . . . . . . . . . . .111131 Manifolds and Accessories . . . . . . . . . . . .132138
111147
CombiZorb Scavengers . . . . . . . . . . . . . . .139141 Sample Filtration . . . . . . . . . . . . . . . . . . . .142147
GC and GC/MS
GC Supplies . . . . . . . . . . . . . . . . . . . . . . . .149168 GC Instrument Parts and Supplies . . . . . . .169198 GC/MS Parts and Supplies . . . . . . . . . . . . .199216
149441
GC and GC/MS Columns . . . . . . . . . . . . . .217323 Troubleshooting/Maintenance . . . . . . . . . .324340 Applications . . . . . . . . . . . . . . . . . . . . . . . .341441
LC and LC/MS
LC Supplies . . . . . . . . . . . . . . . . . . . . . . . .443456 LC Instrument Parts and Supplies . . . . . . . .457500 LC/MS Parts and Supplies . . . . . . . . . . . . .483484
443652
LC and LC/MS Columns . . . . . . . . . . . . . . .501599 Troubleshooting/Maintenance . . . . . . . . . .600603 Applications . . . . . . . . . . . . . . . . . . . . . . . .604652
CE and CE/MS
CE Supplies . . . . . . . . . . . . . . . . . . . . . . . .653654 CE Instrument Parts and Supplies . . . . . . . . . . . .655 CE/MS Instrument Parts and Supplies . . . .656659
653668
Capillaries . . . . . . . . . . . . . . . . . . . . . . . . .660666 Solution Kits . . . . . . . . . . . . . . . . . . . . . . .666668
ICP-MS
7500 ICP-MS Supplies . . . . . . . . . . . . . . . .669678 4500 ICP-MS Supplies . . . . . . . . . . . . . . . .679688
669690
ICP-MS Standards . . . . . . . . . . . . . . . . . . .689690
UV-Vis
Cells and Cell Supplies . . . . . . . . . . . . . . . .691694 Tubing and Fittings . . . . . . . . . . . . . . . . . . . . . . .695 Spectrophotometer Supplies . . . . . . . . . . .696697
691699
Dissolution Testing Supplies . . . . . . . . . . . . . . . .698 Chemical Standards and Accessory Kits . . . . . . .699
Indices
Alphabetical . . . . . . . . . . . . . . . . . . . . . . .701707 Compound . . . . . . . . . . . . . . . . . . . . . . . . .708719
700744
Part Number . . . . . . . . . . . . . . . . . . . . . . .720744
Ordering Information
Easy Ordering Terms and Conditions . . . . . .745746 Custom Column Orders . . . . . . . . . . . . . . .746748
745752
Easy Ways to Order . . . . . . . . . . . . . . . . . .748749 Order Forms . . . . . . . . . . . . . . . . . . . . . . .750752
Information in this document is subject to change without notice.
Agilent Technologies reserves the right to correct any printing or typographical error in this catalog.
Prices in this catalog are for reference only and are subject to change without notice.
Application Information in this catalog is for investigational use only and is not intended to be a complete laboratory diagnostic procedure or method. The performance characteristics of the procedures have not been fully established. The choice of methodology and procedures remains the responsibility of the user and not of Agilent Technologies.
www.agilent.com/chem
Technical Support Information Technical Support at Work for You

You're in the lab and you're busy and something isn't right. When you have a column or supplies technical question, you want the answer NOW. Our Technical Support Specialists are ready to answer your call. These chemists specialize in working together with you to provide the answers you need to keep your laboratory productivity high. Fast answers to your column or supplies technical questions are available from Agilent: 1. Questions pertaining to supplies found in this catalog, call 1-800-227-9770 in the U.S. and Canada or call your local Agilent sales office. Give a brief overview of your questions or dilemma, and a technical expert for that product or process will be right with you. All of our technical support specialists are chemists with years of laboratory experience. They can provide you with in-depth knowledge and experience in consumables and accessories product use and applications. 2. Column selection questions, in the U.S. and Canada, call 1-800-227-9770 and select option 4, 3. Technical Support on the Internet, (Agilent provides world class technical support online at www.agilent.com/chemvoted Best Laboratory Products Manufacturer website in an independent survey conducted at PITTCON.) As the industry leader, Agilent Technologies believes in sharing its wealth of knowledge and information with industry professionals to help you work more effectively and efficiently.
Sales, Service and Support
Instrument Repair Parts Questions

Instrument repair parts are not, for the most part, listed in this catalog. Rather, they are identified in Agilent user or service manuals. If you have questions about instrument repair or require instrument repair information, call 1-800-227-9770, option 3, in the U.S. and Canada. Instrument repair parts are available from the Agilent Support Materials Organization. In the U.S., call 1-877-447-7278 for overnight shipment of repair parts. For repair parts outside the U.S., call your authorized Agilent Technologies distributor or contact your local authorized Agilent service representative.
Training/Services Information Chemical Analysis Training Programs

Agilent Technologies provides carefully planned, effective training courses in using and maintaining Agilent chemical analysis hardware and software products. You can obtain information, see course schedules and register by: Logging on to www.agilent.com/chem and go to the Education link under chemical/biochemical Calling toll-free: 1-800-227-9770 in the U.S. and Canada, or Contacting you Agilent sales representative
Service Bundles Meet Specific Needs for Less Cost

Support Services for Agilent Instruments
Special service bundles contain essential services to keep your chemical analysis instruments in top condition for dependable operation. These bundles cost less than purchasing each service individually.
Petrochemical Bundle
For the specific needs of the petroleum, chemical, petrochemical and other industries not subject to pharmaceutical regulatory compliance, this service bundle provides: Telephone Support to Isolate and Resolve Hardware ProblemsIncluded Without Charge At-Your-Site Service with Consumables used during Repair All Off-Site Instrument Repair Services Annual On-Site Preventive Maintenance
Pharmaceutical Bundle
For the pharmaceutical, agricultural and other industries that must comply with rigorous quality and regulatory requirements, this service bundle provides: Telephone Support to Isolate and Resolve Hardware ProblemsIncluded Without Charge At-Your-Site Service with Consumables used during Repair Annual On-Site Preventive Maintenance Annual Operational Qualification/Performance Verification
Services Information Configure Your Own Support Package

Support Services for Agilent Instruments
You can protect your investment by selecting from a menu of Agilent support services. You can purchase only what you need, for maximum flexibility in meeting your laboratory performance and budget objectives.
Agilent Support Service Repair At-Your-Site Instrument Repair Features Telephone support, with access to highly trained technical support professionals Travel expenses and labor cost for customer service professional Parts for on-site repair A choice of response times Optional Coverage Consumables used during repair Option 1 Replacement with identical instrument the fastest way to resolve a problem Option 2 Return to Agilent for repair Expert cleaning, adjusting, lubricating, or inspecting Travel and labor costs included A predefined checklist of preventive maintenance procedures for consistent service from laboratory to laboratory On-site disassembly Cleaning Re-assembling Testing Advantages to You Fixed annual cost for parts, allowing predictable budget management Selection of response times to suit your uptime and budget requirements
Off-Site Instrument Repair*
Lower-cost support than on-site repair Extensive diagnostic and testing that are not feasible with on-site maintenance or repair
Maintenance Preventive Maintenance*
Maximized instrument uptime Extended instrument life Confidence in the integrity of your measurements
Mass Spectrometer Ion Source Cleaning*
Laboratory staff can devote more time to chemistry, not instrument cleaning Efficient cleaning for minimum downtime The evidence needed to help you satisfy regulatory agencies No need to write your own standard operating procedures to qualify Agilent instruments and train your staff Consistent and traceable results among all your laboratories and improved method transfer Confidence in the integrity of your measurements, with traceable, documented chains from sample introduction to reporting Fast problem identification and resolution Fixed annual cost for software updates, for predictable budget management Timely and convenient way to remain current with the latest features, enhancing laboratory capability Cost-effective coverage for additional, identical systems at a single location
*For selected Agilent instruments
Qualification Operational Qualification/ Performance Verification*
Verification and documentation of an instruments ability to meet specified criteria Procedures and documentation that fit the requirements of GLP, ISO 9000, and other regulatory agencies Measuring equipment traceable to national and international standards Preventive maintenance is recommended before Operational Qualification/Performance Verification Telephone assistance from highly trained technical professionals to isolate and resolve software problems All enhancements to the original chemical analysis application software purchase Agilent software status bulletins that identify discovered defects and recommend workarounds
Software
Telephone Support and Updates for Software
Agilent Offices Worldwide

Argentina
Agilent Technologies Av. Crdoba 1131 (C1055AAB) Capital Federal, Argentina Phone: 5811-7260 Fax: 5811-7126 analitica@agilent.com
China
Agilent Technologies Co. Ltd (China) 15F, Hua Xin International Building 219 Qingnian Street Shenyang 110015, Liaoning Province P R China 100022 Phone: 86-24-23-964688 Fax: 86-24-23-964788 Agilent Technologies Co. Ltd (China) HP Building, 8th Floor No. 2 Dong San Huan Nan Lu Chao Yang District, Beijing 100022 P R China Phone: 8610-6564-5513 Fax: 8610-6566-8330 Agilent Technologies Co. Ltd (China) Da Ye Building, 22nd Floor No. 39 Da Ya Road Chengdu 610016 P R China Phone: 86-28-665-5500 Fax: 86-28-667-4321 Agilent Technologies Co. Ltd (China) Unit 08, 66F Citic Plaza 233 Tian He Bei Road Guangzhou 510613 P R China Phone: 86-20-8668-5500 Fax: 86-20-8669-5861 Agilent Technologies Co. Ltd (China) Level 12 Novel Building 887 Huai Hai Zhong Lu Shanghai 200020 P R China Phone: 86-21-6474-5500 Fax: 86-21-6474-8307
Finland
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Japan
Yokogawa Analytical Systems Inc. 1-15-5 Nakacho, Musashino-shi Tokyo 180 8543 Phone: 0120-477-111
Korea
Agilent Technologies Korea Ltd 25-12, Yoido-dong Youngdeungpo-gu Chemical Analysis Group (CAG) Unit no 201, level 2, Uptown 2 2 Jalan SS 21/37, Damansara Uptown 47400 Petaling Seoul 150-010 Phone: 822-2004-5096 Fax: 822-2004-5089
Australia
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France
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Austria
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Germany
Agilent Technologies Deutschland GmbH Chemische Analysentechnik Hewlett-Packard-Str. 8 76337 Waldbronn Phone: 0800-603 1000 Fax: 07243-602602 analytik_germany@agilent.com www.agilent.de
Malaysia
Agilent Technologies Sales (M) Sdn Bhd Jaya Selangor Darul Ehsan West Malaysia Phone: 603-1-800-88-0805 Fax: 603-7727-1239
Netherlands
Agilent Technologies Netherlands B.V. Startbaan 16 1187 XR Amstelveen Phone: 020 547 2600 Fax: 020 547 2690 chem_benelux@agilent.com
Belgium
Agilent Technologies Belgium SA/NV Boulevard De La Woluwe 100 1200 Brussels Phone: +32 (0)2-4049222 Fax: +32 (0)2-4049290 cag_belgium@agilent.com
Hong Kong
Agilent Technologies Hong Kong Ltd 24th Floor, Cityplaza One 1111 King's Road Taikoo Shing Phone: 852-3197-7341 Fax: 852-2506-9275
Norway
Agilent Technologies Norway AS Drammensveien 169 0277 OSLO Phone: 815 32 560 Fax: 2325 3710 kjemisk_analyse@agilent.com www.agilent.com/chem
Brazil
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Canada
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Ireland
Agilent Technologies Ireland Ltd. Silverstone House Ballymoss Road Sandyford Dublin 18 Phone: 01 605 8324 Fax: 01 605 8323 cag_enquiry@agilent.com
Poland
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Dominican Republic
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Singapore
Agilent Technologies Singapore (Sales) Pte Ltd Blk B #05-12 Alexandra Technopark 438-B Alexandra Road Singapore 119968 Phone: 65-215-6316 Fax: 65-271 0423
Italy
Agilent Technologies Italia S.p.A. Phone: 800 01 2575 (numero verde) Fax: 02-9210 4575 analitica_italia@agilent.com
Agilent Offices Worldwide

Spain
Agilent Technologies Spain, S.L. Grupo de Anlisis Qumico Ctra. N-VI, Km 18,200 28230 Las Rozas, Madrid Phone: 901 11 68 90 Fax: 34 916 376 505 cag_instalaciones@agilent.com
United Kingdom
Agilent Technologies UK Limited Lakeside Cheadle Royal Business Park Stockport Cheshire SK8 3GR Phone: 0845 712 5292 Fax: 0161 492 7171 cag_enquiry@agilent.com
Sweden
Agilent Technologies Sweden AB, Box 52, 164 94 Kista Phone: 08 506 489 50 Fax: 08 506 489 51
United States
Agilent Technologies, Chemical Analysis Group North American Sales, Customer Contact Center, 2850 Centerville Road Wilmington, Delaware 19808 Phone: 800-227-9770 Fax: 302-633-8901 cag_sales-na@agilent.com www.agilent.com/chem
Switzerland
Agilent Technologies (Schweiz AG) Chemische Analysentechnik In den Luberzen 29 8902 Urdorf Phone: 0848 803 560 Fax: 01 735 97 44 cag_qualify@agilent.com www.agilent.ch
Venezuela
Agilent Technologies de Venezuela C.A Av. Francisco de Miranda Torre HP, Piso 12 Caracas, CP 01060 Phone: (58212)-209-4613 Fax: (58212)-209-4618 vilma_bastidas@agilent.com
Taiwan
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Vietnam
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Thailand
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Note: For a complete listing of authorized distributors, visit our website www.agilent.com/chem or contact your nearest Agilent office.
Web Information
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Featured Products Demonstrating the Value of Deep Knowledge

Were calling them Featured Products , but they are much more than that. They represent the whole new approach that Agilent is taking to its business. We realize that you dont just need the highest quality instruments, columns, and supplies; you need a partner who can bring you solutions based on long experience and expert insight. Thats what each of the products in this section represents. From an item as specific as a patented new vial for extreme recovery, to the comprehensive information we pack into our website, each is a way for Agilent to share 35 years of daily work by hundreds of technical experts in your application areaon the instruments you use. Better still, each product is designed to make your job easier and your lab more productive, which is what a true partnership is all about.
Featured Products
Zorbax SPE Products
2002 Featured Products

New 96 well plates and cover mats for well plate autosamplers: Sample Preparation Section, page 113 Micro-V economical microsampling vials for Agilent autosamplers: General Chromatography, pages 16, 21, 25 Zorbax Solid Phase Extraction sample preparation products: Sample Preparation, page 114 AccuBONDII SPE cartridges in a wide variety of phases: Sample Preparation, page 120 Extreme Recovery 1.5 mL crimp cap vials: General Chromatography, page 22 Interseal caps with bonded septa for 2 mL screw top vials: General Chromatography, page 17 Electronic crimpers and decappers for 2 mL and 20 mm vials: General Chromatography, page 37 Expanded offering of gas purification devices, including the Big Universal Trap: General Chromatography, page 47 Comprehensive line of flowmeters from the classic digital bubble meters to combination flowmeter/leak detector, the FlowTracker 2000: General Chromatography, page 73 The new consolidated J&W Scientific brand of GC columns: GC and GC/MS Section, pages 217323 Poroshell particle design HPLC columns for high velocity and high resolution of protein separations: LC and LC/MS Section, pages 535536 Gel filtration chromatography columns from Shodex: LC and LC/MS Section, page 586587 Zorbax SB-Aq Rapid Resolution LC columns for polar analytes: LC and LC/MS Section, page 530531 100 mm LC columns: LC and LC/MS Column Section Agilent MicroBore and Capillary LC columns: LC and LC/MS Section, pages 560561 Zorbax 300Extend LC columns for excellent resolution and long lasting stability from pH 2 to 11.5: LC and LC/MS Section, pages 547559 Agilent Chemical Analysis Website: www.agilent.com/chem
Screw caps with Integrated Septa
Electronic Crimper and Decapper
GC Capillary Column
General Chromatography
Table of Contents
General Chromatography Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .11 Vials, Caps and Septa . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .12 Septum Selection Guide . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .13 Vial Identification Chart . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .14 Screw Top Vials, Caps, Septa and Kits . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1519 Crimp Top Vials, Caps and Kits . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2023 Snap Top Vials, Caps and Kits . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2427 Wide Opening Inserts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2728 8-425 Screw Top Vials, Caps, Septa and Inserts . . . . . . . . . . . . . . . . . . . . . . . . . .2931 4 mL Vials, Caps and Septa . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .32 Headspace Crimp Top Vials, Caps, Septa and Kits . . . . . . . . . . . . . . . . . . . . . . . . .3335 General Purpose Sample and Storage Vials . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3536 Crimping and Decapping Tools . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3738 Vial Racks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .38 Syringes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .39 Autosampler Syringes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4042 Manual Syringes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4346 Gas Management Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .47 Gas Purifier Selection Guide . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4849 Gas Purifier Capacity Chart . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .50 Moisture Traps . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5155 Oxygen Traps . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5657 Hydrocarbon Traps . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5759 Combination Traps . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6062 Gas Purification Systems, Accessories and Kits . . . . . . . . . . . . . . . . . . . . . . . . . . .6370 GC Installation Kits . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7172 Flowmeter Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .73 Flowmeter Selection Guide . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .74 Flowmeters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7579 Leak Detectors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .80 Regulators and Accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .8182 Hardware . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .8391 Standards . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .92 GPC/GFC Standards . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .92 CE and UV-Vis Standards . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .9394 HPLC, LC/MS and RI Standards . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .9495 GC Column Test Standards . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .9697 GC and GC/MS Instrument Standards . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .98101 Integrators, Integrator Supplies and Cables . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .102106 Books, Learning Software and CDs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .107109
10
General Supplies
Making Your Chromatography Lab More Productive

Labs run smoothly when every detail is right. With Agilent supplies and accessories, you can be confident that they are. All of our products have been designed or selected by our instrument design teams, manufactured to our demanding specifications, and tested in a variety of conditions to ensure a PerfectFit with Agilent instruments. That painstaking care registered to ISO 9001ensures that Agilent instruments will perform at optimum levels when you use Agilent products. In the following pages, you'll find what you need to make your lab more productiveday after day. You'll find the staples, from caps and vials to manual and autosampler syringes. You'll find gas management supplies, such as purifiers, regulators, flowmeters, and leak detectors. Hardware. Standards. Plus products that directly share our product and application knowledge with youthe latest in technical publications, learning software, and CDsto give you a competitive edge.
11
Vials
Vials, Caps and Septa
Vials, Caps and Septa

All Agilent Vials use USP Type 1 Borosilicate Glass
Agilent Technologies vials are made only from Type 1 Borosilicate glass and are tightly controlled for dimensional accuracy using the Optoelectronic system. The vials come in crimp top, screw top or snap top closure and are also available in deactivated or non-deactivated versions. Before doing an injection, you should think about: Type of closure: Crimp top vials, screw top vials or snap top vials While crimp tops are the least expensive, they need a crimping tool and the caps are not reusable. Screw top vials are the perfect medium for reusability and low evaporation.
Type of glass or polypropylene: Clear or amber glass, polypropylene vials If your sample is light sensitive, you may choose amber glass. For most applications, standard clear glass is sufficient. For samples that may stick to glass or chemically react with glass, use polypropylene. Deactivated/non-deactivated: For samples that interact with the highly polar silanol groups on the glass surface, a deactivation procedure is performed on the vials. Need for inserts: Flat or conical with polyspring If you only have a small amount of sample, you may choose to place a limited volume insert inside the vial or choose micro sampling vials like Micro-V or High Recovery. Polyspring at the bottom of the conical insert keeps it aligned for proper injection. Type of septa: Depends on the properties of your sample, use the septum selection guide on the following page.
TIGHTLY CONTROLLED DIMENSIONS 12 mm
40 % LARGER OPENING THAN THE STANDARD MOUTH VIAL
OPTIONAL WRITE-ON PATCH WITH GRADUATIONS
1.5 mL
32 mm
Wide Opening 2 mL Vial
FLAT BOTTOM FOR INSERT STABILITY
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Vials
General Information/Vials, Caps and Septa
Autosampler Compatibility Chart: Agilent wide opening vials are compatible with
Agilent as well as non-Agilent autosamplers.
Autosampler Agilent Technologies Beckman Dynatech Finnigan Fisons Gilson Hewlett-Packard Hitachi L.E.A.P. LDC Perkin-Elmer Spark Spectra-Physics Varian Waters Model All Models Except CE, that cannot be pressurized 501, 507 42 Vial Tray, LC 2000 A200S AS 800 231-401, 232-402, 201/202, 221/222, Aspec All Models Except CE AS-2000, AS-4000
A200S Marathon, Promis 4900, Integral 4000, Autosystem GC, ISS 100/ISS 200, LC 600 42-Tray Marathon/Promis, SPH 125 SpectraSystem, 8875/8880 8100/8200, 8035/9095, 8000, Marathon, Vista 2690, Alliance Systems
Caps and Septa

Vial cap septa are a critical link to optimal analysis. Each septum is specified to complement the overall system and enhance chemical performance. As with all Agilent components, rigorous testing and product specifications are done before offering the product. Agilent vial cap septa are specially formulated and constructed to provide optimum system performance with minimal coring and superior chemical inertness. The softness or hardness (durometer) is matched to the instrument and designed to work well with syringes and samples. The size of the septum is critical to proper sealing. All Agilent septa are specifically sized for proper sealing. Our thinnest septa have a special detail built into the cap to minimize septa coring and eliminate the need to replace caps with extended usage.
Septum Selection Guide

Septum Material Compatible with Incompatible with Resealability Max. Temperature
Rubber (Natural/Butyl) PTFE/Natural or Butyl Rubber Silicone/Silicone Rubber PTFE/Silicone, PTFE/Silicone/PTFE VITON
ACN, acetone, DMF, alcohols, diethylamine, DMSO, phenols PTFE resistance until punctured, then septa or liner will have compatibility of rubber Alcohol, acetone, ether, DMF, DMSO PTFE resistance until punctured, then septa will have compatibility of silicone Chlorinated solvents, benzene, toluene, alcohols, hexane, heptane
Chlorinated solvents, aromatics, hydrocarbons, carbon disulfide
Excellent Good
< 100C < 100C < 200C < 200C < 260C
ACN, THF, benzene chloroform, Excellent pyridine, toluene, hexane, heptane Average DMF, DMSO, ACN, THF, pyridine, dioxane, methanol, acetone Good
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Identification Chart
Agilent Vial Identification Chart and Actual Fill Volumes

Wide Opening Screw Top Vials: Septum Diameter 9 mm Wide Opening Crimp Top Vials: Septum Diameter 11 mm
100 L 32 mm x 12 mm
300 L
100 L
1.5 mL
1.5 mL
1.5 mL
1.5 mL
1.0 mL
51820714
51832030
51813375 Wide Opening Snap Top Vials
51823454
51820567
93010978
51834731
93010977
51843551
51855821
Inserts for 2 mL,12x32 mm Wide Opening Vials 100 L 100 L 250 L
300 L
100 L
100 L 31 mm
1.5 mL
1.5 mL
1.0 mL
51820544
51834510
51820567
51834731
93010978
93010977
51811270
51832085
51813377
Narrow Opening Screw Top Vials
Inserts for 2 mL, 12x32 mm Narrow Opening Screw Top Vials 100 L 100 L 250 L
1.5 mL
51834428 Headspace Crimp Top VialsFlat and Rounded Bottom
51832088
51832089
51832090
Vials for Agilent LC Instruments
True scale: 75 mm x 22 mm
20 mL True scale: 45 mm x 22 mm
20 mL 500 l 700 L 6 mL
10 mL
10 mL
51820837
51820838
51834474
51834475
51800806
51800805
93011377
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Screw Top Vials
Agilent Screw Top Vials, Caps, Septa and Kits

Combine the excellent autosampler handling of a crimp cap profile with the ease of a screw cap. The screw caps and precision fit septa give a secure seal with the microvolume inserts.
Highlights
2 mL, 12 x 32 mm 40% larger opening than standard narrow opening vials Unique thread design for consistently secure seal (9 x 425) Precision-formed neck for optimal robotic arm handling
Rigorous quality assurance for dimensional consistency from lot to lot Uniformly flat bottom ensures compatibility with inserts Optional ceramic write-on spot with fill marks Select from several compatible microvolume inserts
Screw Top Vials

Wide Opening Screw Top Glass Vials
Versatile 2 mL, 12 x 32 mm wide opening vials designed for autosamplers with rotating or robotic arm trays.
Screw Top, 2 mL Vials Clear wide opening screw top Clear wide opening screw top, write-on spot Amber wide opening screw top, write-on spot Clear wide opening screw top Clear wide opening screw top, write-on spot Amber wide opening screw top, write-on spot Unit 100/pk 100/pk 100/pk 1000/cs* 1000/cs* 1000/cs* Part No. 5182-0714 5182-0715 5182-0716 5183-2067 5183-2068 5183-2069
*Case includes 10 packs of 100 vials
P/N 5182-0714, 5182-0715, 5182-0716
Deactivated Wide Opening Screw Top Glass Vials

These wide opening 2 mL, 12 x 32 mm vials are created using a deactivation process to eliminate sample interactions. Ready-to-use for the analysis of polar compounds such as phenols, proteins, antibodies, amines, metabolites, herbicides and pesticides.
Deactivated Screw Top, 2 mL Vials Clear deactivated wide opening screw top Clear deactivated wide opening screw top, write-on spot Unit 100/pk 100/pk Part No. 5183-2070 5183-2071 5183-2072
P/N 5183-2070, 5183-2071, 5183-2072
Amber deactivated wide opening screw top, write-on spot 100/pk
Helpful Hint: Microvolume inserts are shown on pages 2728.
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Screw Top Vials and Caps 1.5 mL Wide Opening Screw Top High Recovery Glass Vials
Wide opening, 1.5 mL vials with 30 L reservoir. For sample concentration and injection without transferring to microvolume inserts.
Screw Top High Recovery Vials Clear high recovery screw top Amber high recovery screw top, write-on spot Unit 100/pk 100/pk Part No. 5183-2030 5183-2073
P/N 5183-2030, 5183-2073
Screw Top Micro-V Vial
12 x 32 mm, Type 1 Borosilicate glass Compatible with standard screw closures 1.5 mL total volume Less than 5 L dead volume Designed for center needle position during sample injection (not recommended for Vortex)
Screw Top Micro-V Vials Micro-V vial, clear, screw top
P/N 5184-3550
Unit 100/pk 100/pk
Part No. 5184-3550 5184-3554
Micro-V vial, amber, screw top
Screw Caps
Polypropylene Screw Caps with Septa for Wide Opening Screw Top Vials
These precision-fit septa provide an evaporation proof seal. (See the Septum Selection Guide to choose the septum best suited for your application.)
Screw Caps Blue, PTFE/red silicone rubber septa* Green, PTFE/red silicone rubber septa* Red, PTFE/red silicone rubber septa* Black, PTFE/red silicone rubber septa* Blue, PTFE/silicone septa Green, PTFE/silicone septa
P/N 5182-0717, 5182-0718, 5182-0719
Unit 100/pk 100/pk 100/pk 100/pk 100/pk 100/pk 100/pk 100/pk 100/pk 100/pk 100/pk 100/pk 100/pk
Part No. 5182-0717 5182-0718 5182-0719 5185-5838 5182-0720 5182-0721 5182-0722 5182-0723 5182-0724 5182-0725 5183-2076 5183-2077 5183-2078
*Commonly referred to as red rubber
Red, PTFE/silicone septa Blue, PTFE/silicone/PTFE septa Green, PTFE/silicone/PTFE septa Red, PTFE/silicone/PTFE septa Blue, pre-slit PTFE/silicone septa Green, pre-slit PTFE/silicone septa Red, pre-slit PTFE/silicone septa
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Vials
Screw Caps and Septa New Screw Caps with Integrated Septa
Bonded caps combine innovation with time saving convenience. This chemically inert patented process produces a true bond at the molecular level of the septa and plastic cap, eliminating excess evaporation and providing proper seal with the vial. Septa are completely secure and will not fall into the vial during injection. These caps are made with PTFE/Silicone septa for excellent chemical resistance and multiple injections.
Screw Caps with Integrated Septa Blue polypropylene cap, bonded PTFE/silicone septa Unit 100/pk Part No. 5185-5823 5185-5824
Blue polypropylene cap, bonded pre-slit PTFE/silicone septa 100/pk
P/N 5185-5823
Polypropylene Screw Caps (no septa)

An economical choice. Use caps and replace septa when needed. Choose from three cap colors for separating assays, lots, standards, etc.
Screw Caps without Septa Blue open top screw caps Green open top screw caps Red open top screw caps Blue PTFE-lined solid top screw caps (for storage) Unit 100/pk 100/pk 100/pk 100/pk Part No. 5182-0728 5182-0727 5182-0726 5183-2075
P/N 5182-0728, 5182-0727, 5182-0726
Septa
Septa for Wide Opening Screw Caps
Septa for Screw Caps Ivory PTFE/red silicone rubber septa* Red PTFE/white silicone/red PTFE septa Blue pre-slit PTFE/white silicone septa Red PTFE/white silicone septa Unit 100/pk 100/pk 100/pk 100/pk Part No. 5182-0731 5182-0729 5183-2074 5182-0730
Assortment of Septa for Wide Opening Screw Caps
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Screw Top Vial Kits
Vial Kits
Non-Assembled, 2 mL Wide Opening Screw Top Glass Vial Convenience Packs
Screw Top Convenience Packs are an easy way to get 500 of each component using one catalog number. Packed in our six drawer reusable blue plastic cabinet, 500 screw top vials and screw caps with septa installed are kept handy and dust-free. Note: Review the Septum Selection Guide to choose the septum best suited for your application.
Screw Top Vial Kit
Screw Top Vials and Caps Kits Clear vials with write-on spot, blue screw caps, PTFE/red silicone rubber septa* Amber vials with write-on spot, green screw caps, PTFE/red silicone rubber septa* Clear vials, blue screw caps, PTFE/silicone/PTFE septa Clear vials with write-on spot, blue screw caps, PTFE/silicone/PTFE septa Amber vials with write-on spot, green screw caps, PTFE/silicone/PTFE septa Clear vials, blue screw caps, PTFE/silicone septa Clear vials with write-on spot, blue screw caps, PTFE/silicone septa Amber vials with write-on spot, green screw caps, PTFE/silicone septa Clear vials, blue screw caps, pre-slit blue PTFE silicone septa Clear vials with write-on spot, blue screw caps, pre-slit blue PTFE silicone septa Amber vials with write-on spot, green screw caps, pre-slit blue PTFE silicone septa
Unit 500/pk 500/pk 500/pk 500/pk 500/pk 500/pk 500/pk 500/pk 500/pk 500/pk 500/pk
Part No. 5182-0732 5182-0867 5182-0733 5182-0736 5182-0869 5182-0737 5182-0734 5182-0868 5182-0735 5183-2079 5183-2080 5183-2081
Clear vials, blue screw caps, PTFE/red silicone rubber septa* 500/pk
Helpful Hint: For LC specific vials please refer to the LC supplies section.
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Vials
Screw Top Vial Kits Pre-Assembled, 2 mL Wide Opening Screw Top Glass Vial Packs
Screw Top Vial Pre-assembled Packs come ready to use with cap and septum of your choice attached to the vial. A time/labor-saving product for use on your Agilent Autosampler or any rotating tray automatic sampler. Note: Review the Septum Selection Guide to choose the septum best suited for your application.
Pre-Assembled Screw Top Kits Clear vials with write-on spot, blue screw caps, PTFE/red silicone rubber septa* Amber vials with write-on spot, green screw caps, PTFE/red silicone rubber septa* Clear vials, blue screw caps, PTFE/silicone/PTFE septa Clear vials with write-on spot, blue screw caps, PTFE/silicone/PTFE septa Amber vials with write-on spot, green screw caps, PTFE/silicone/PTFE septa Clear vials, blue screw caps, PTFE/silicone septa Clear vials with write-on spot, blue screw caps, PTFE/silicone septa Amber vials with write-on spot, green screw caps, PTFE/silicone septa Clear vials, blue screw caps, pre-slit blue PTFE silicone septa Clear vials with write-on spot, blue screw caps, pre-slit blue PTFE silicone septa Unit 100/pk 100/pk 100/pk 100/pk 100/pk 100/pk 100/pk 100/pk 100/pk 100/pk Part No. 5182-0553 5182-0864 5182-0554 5182-0555 5182-0866 5182-0556 5182-0557 5182-0865 5182-0558 5183-2082 5183-2083
Preassembled Vial Pack
Clear vials, blue screw caps, PTFE/red silicone rubber septa* 100/pk
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Vials
Crimp Top Vials
Agilent Crimp Top Vials, Caps and Kits

The wide opening crimp cap provides a larger target area for improved auto-sampler needle accuracy. Select from four cap colors and a variety of septa. Whatever your crimp top vial needs, Agilent has what you are looking for.
Highlights
2 mL, 12 x 32 mm Tightly controlled crown for improved crimping Precision-formed neck for improved autosampler handling 40% larger opening than standard narrow opening vials Rigorous quality assurance provides dimensional consistency from lot to lot Uniformly flat bottom ensures compatibility with inserts Optional ceramic write-on spot with fill marks
Vials
Wide Opening Crimp Top Glass Vials
Wide opening 2 mL, 12 x 32 mm vials. The outer diameter of the crimp head is 11 mm. Microvolume inserts for microsampling are shown on pages 2728.
Crimp Top, 2 mL Vials Clear wide opening crimp top Clear wide opening crimp top, write-on spot Amber wide opening crimp top, write-on spot Clear wide opening crimp top Clear wide opening crimp top, write-on spot
P/N 5181-3375, 5182-0543, 5181-3376
Unit 100/pk 100/pk 100/pk 1000/cs* 1000/cs* 1000/cs*
Part No. 5181-3375 5182-0543 5181-3376 5183-4491 5183-4492 5183-4493

Amber wide opening crimp top, write-on spot
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Vials
Crimp Top Vials Deactivated Wide Opening Crimp Top Glass Vials
The same vials as described above with the addition of a deactivation process to eliminate sample interactions. Ready-to-use for polar compound analysis such as phenols, proteins, antibodies, amines, metabolites, herbicides and pesticides.
Deactivated Crimp Top Vials Clear deactivated wide opening crimp top, 2 mL Clear deactivated wide opening crimp top, write-on spot, 2 mL Amber deactivated wide opening crimp top, write-on spot, 2 mL Unit 100/pk 100/pk 100/pk Part No. 5183-4494 5183-4495 5183-4496
P/N 5183-4494, 5183-4495, 5183-4496
1.5 mL Wide Opening Crimp Top High Recovery Glass Vials

Wide opening, 1.5 mL vials with 30 L reservoir. For sample concentration and injection without transferring to microvolume inserts.
High Recovery Crimp Top Vials Clear high recovery wide opening crimp top, 1.5 mL with 30 L reservoir Clear deactivated high recovery wide opening crimp top, 1.5 mL with 30 L reservoir Unit 100/pk 100/pk Part No. 5182-3454 5183-4497
P/N 5182-3454
Crimp Top Micro-V Vial

12 x 32 mm, Type 1 Borosilicate glass Compatible with standard crimp closures 1.5 mL total volume Less than 5 L dead volume Designed for center needle position during sample injection Not recommended for Vortex or in-vial sample concentration
Micro-V Crimp Top Vials Micro-V vial, clear, crimp top Micro-V vial, amber, crimp Unit 100/pk 100/pk Part No. 5184-3551 5184-3555
P/N 5184-3551
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Vials
Crimp Top Vials New Extreme Recovery Vial
Patented design with stable base for GC autosamplers Uniform internal surface without any ridges to hold back sample It has wider neck opening for easy pipetting Increased sample volume capacity versus standard inserts Low residual volume for smaller volume injections without using the inserts Safe to use with Vortex for sample concentration
Extreme Recovery Crimp Top Vials
P/N 5185-5821
Unit 100/pk
Part No. 5185-5821
1.5 mL Extreme Recovery clear crimp top vial
Wide Opening Crimp Top Polypropylene Vials

Wide opening 12 x 32 mm vials manufactured from virgin polypropylene, meeting the requirements of 21 CFR 177.1520. Polypropylene is chemically resistant and the material of choice for pH sensitive samples, sodium or heavy metals analysis. Polypropylene is translucent and able to withstand temperatures to 135C. Use with crimp or snap caps.
Polypropylene Crimp Top Vials Polypropylene wide opening crimp top, 1 mL
P/N 5182-0567, 9301-0978
Unit 100/pk
Part No. 5182-0567
Polypropylene wide opening crimp top, 300 L
1000/pk 9301-0978
Wide Opening Crimp Top Glass/Plastic Vials

Most popular 12 x 32 mm wide opening vials for economical and effective microsampling. Polypropylene body with a glass flanged insert molded to the inside. Sample comes in contact only with the 100 L high quality glass insert and the septum. Use with crimp or snap caps. Note: Microvolume inserts are shown on pages 2728.
Glass/Plastic Crimp Top Vials
P/N 9301-0977
Unit
Part No. 9301-0977
Glass lined polypropylene wide opening crimp top, 100 L 100/pk
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Vials
Crimp Caps/Vial Kits
Caps
Crimp Caps with Septa
Note: Review the Septum Selection Guide to choose the septum best suited for your application.
11 mm Crimp Caps with Septa Silver aluminum, clear PTFE/red natural rubber septa Blue aluminum, clear PTFE/red natural rubber septa Green aluminum, clear PTFE/red natural rubber septa Red aluminum, clear PTFE/red natural rubber septa Silver aluminum, PTFE/silicone/PTFE septa Silver aluminum, PTFE/silicone septa Silver aluminum, black Viton septa Silver aluminum, clear PTFE/red natural rubber septa Silver aluminum, PTFE/silicone/PTFE septa Silver aluminum, PTFE/silicone septa Silver aluminum, PTFE septa Unit 100/pk 100/pk 100/pk 100/pk 100/pk 100/pk 100/pk 1000/pk 1000/pk 1000/pk 1000/pk Part No. 5181-1210 5181-1215 5181-1216 5181-1217 5181-1211 5182-0552 5181-1212 5183-4498 5183-4499 5183-4500 5182-0871
P/N 5181-1210, 5181-1215, 5181-1216, 5181-1217
Vial Kits
Non-Assembled, 2 mL Wide Opening Crimp Top Glass Vial Convenience Packs
Crimp Top Vial Convenience Packs are an easy way to get 500 of each vial component using one catalog number. Packed in our six drawer reusable blue plastic cabinet, all items are kept handy and dust-free.
Crimp Top Vials and Cap Kits Clear crimp top vials, silver aluminum crimp caps, PTFE/ red rubber septa Amber crimp top vials with write-on spot, silver aluminum crimp caps, PTFE/red rubber septa Unit 500/pk 500/pk Part No. 5181-3400 5181-8801
Crimp Vial Kit
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Snap Top Vials
Agilent Snap Top Vials, Caps and Kits

The simple, efficient way to a secure seal without crimping. It's as easy as push-on, pull-off. A consistently secure seal outperforms the loose fit of other snap caps. A variety of snap caps with septa are available on page 26. Crimp caps can also be used with wide opening snap top vials. Snap cap vials cannot be pressurized. For microsampling, see microvolume inserts for wide opening vials.
Highlights
2 mL, 12 x 32 mm Use with any 11 mm snap or crimp cap Provides a total seal around the entire circumference of the vial crown 40% larger opening than standard narrow opening vials Precision-formed neck improves autosampler handling Optional ceramic write-on spot Uniformly flat bottom ensures compatibility with inserts
Vials
Wide Opening Snap Top Glass Vials
Wide opening 2 mL, 12 x 32 mm vials. Microvolume inserts for microsampling are shown on pages 2728. Use with snap or crimp caps.
Snap Top Vials Clear wide opening snap top, 2 mL
P/N 5182-0544, 5182-0546, 5182-0545
Unit 100/pk 100/pk 100/pk 1000/cs* 1000/cs* 1000/cs*
Part No. 5182-0544 5182-0546 5182-0545 5183-4504 5183-4505 5183-4506

Clear wide opening snap top, write-on spot, 2 mL Amber wide opening snap top, write-on spot, 2 mL Clear wide opening snap top, 2 mL Clear wide opening snap top, write-on spot, 2 mL Amber wide opening snap top, write-on spot, 2 mL
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Vials
Snap Top Vials Deactivated Wide Opening Snap Top Glass Vials
The 2 mL vials undergo a deactivation process to eliminate sample interactions. Ready-to-use for the analysis of polar compounds such as phenols, proteins, antibodies, amines, metabolites, herbicides and pesticides.
Deactivated Snap Top Vials (100/Pk) Clear deactivated wide opening snap top, 2 mL Clear deactivated wide opening snap top, write-on spot, 2 mL Amber deactivated wide opening snap top, write-on spot, 2 mL Part No. 5183-4507 5183-4508 5183-4509
P/N 5183-4507, 5183-4508, 5183-4509
Wide Opening Snap Top High Recovery Glass Vials
Wide opening, 1.5 mL vials with 30 L reservoir. For sample concentration and injection without transferring to microvolume inserts. Use with snap or crimp caps.
Snap Top High Recovery Glass Vial (100/Pk) Clear high recovery snap top, 1.5 mL with 30 L reservoir Part No. 5183-4510
P/N 5183-4510
Snap Top Micro-V Vial

12 x 32 mm, Type 1 Borosilicate glass Compatible with standard snap or crimp closures 1.5 mL total volume Less than 5 L dead volume Designed for center needle position during sample injection Not recommended for Vortex or in-vial sample concentration
Snap Top Micro-V Vials Micro-V vial, clear, snap top Micro-V vial, amber, snap top Unit 100/pk 100/pk Part No. 5184-3552 5184-3556
P/N 5184-3550, 5184-3551
Wide Opening Snap Top Polypropylene Vials

Wide opening 12 x 32 mm vials manufactured from virgin polypropylene, meeting the requirements of 21 CFR 177.1520. Polypropylene is chemically resistant and the material of choice for pH sensitive samples, sodium or heavy metals analysis. Polypropylene is translucent and able to withstand temperatures to 135C. Use with snap or crimp caps.
Snap Top Polypropylene Vials Polypropylene wide opening snap top, 1 mL Polypropylene wide opening snap top, 300 L Polypropylene wide opening snap top, 300 L Unit 100/pk 1000/pk 1000/pk Part No. 5182-0567 9301-0978 5183-4731
P/N 5182-0567, 9301-0978
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Vials
Snap Top Vials and Caps Wide Opening Snap Top Glass/Plastic Vials
The most popular 2 mL, 12 x 32 mm wide opening vials for economical and effective microsampling. Polypropylene body with a glass flanged insert molded to the inside. Sample comes in contact only with the 100 L high quality glass insert and the septum. Use with snap or crimp caps.
Snap Top Glass/Plastic Vials (100/Pk) Glass lined polypropylene wide opening snap top, 100 L
P/N 9301-0977
Part No. 9301-0977
Helpful Hint: For a low cost alternative, use microvolume inserts shown on page 28.
Caps
Snap Caps with 11 mm Septa
Review the Septum Selection Guide to choose the septum best suited for your application.
Snap Caps, 11 mm (100/Pk) Clear polyethylene cap, solid polyethylene membrane septa Clear polyethylene cap, clear PTFE/red silicone rubber septa* Blue polyethylene cap, clear PTFE/red silicone rubber septa* Green polyethylene cap, clear PTFE/red silicone rubber septa* Red polyethylene cap, clear PTFE/red silicone rubber septa* Clear polyethylene cap, PTFE septa
P/N 5182-0542, 5182-0550, 5182-3458, 5182-3457, 5182-3459
Part No. 5182-0542 5182-0550 5182-3458 5182-3457 5182-3459 5182-0540 5182-0566 5182-0541 5183-4511 5182-0870
Clear polyethylene cap, PTFE/silicone/PTFE septa Blue polyethylene cap, PTFE/silicone septa Clear polyethylene cap, pre-slit PTFE/silicone septa Clear polyethylene cap, Viton septa
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Vials
Snap Top Vials and Caps/Wide Opening Inserts
Vial Kits
Non-Assembled, 2 mL Wide Opening Snap Top Glass Vial Convenience Packs
Snap top convenience packs are an easy way to get 500 of each vial component using one catalog number. Packed in our six drawer reusable blue plastic cabinet, snap top vials and snap caps with septa are kept handy and dust-free.
Snap Top Vials and Caps Kits Clear snap top vials, clear polypropylene snap caps, PTFE/red rubber septa Amber snap top vials with write-on spot, clear polypropylene snap caps, PTFE/red rubber septa Part No. 5182-0547 5182-0548
Snap Top Vial Kit
Agilent Microvolume Inserts for Wide Opening Vials

To meet your microsampling needs, Agilent has a variety of microvolume inserts with capacities and designs that can transform our vials from full-capacity to limited volume with one simple step. These inserts are compatible with our wide opening screw top, crimp top, and snap top vials.
Insert Highlights
Conical Inserts with Polymer Feet Feet act as shock absorber for needle Dead-volume is virtually eliminated Inserts are self-aligning and stand straight Flat Bottom Inserts Custom-fit to vial Extremely flat bottomno push-up Conical Glass Inserts Pulled-point interior Economical alternative
27
Vials
Wide Opening Inserts Conical Inserts with Polymer Feet
With the correct default setting on an autosampler, these inserts have practically no dead volume. The syringe needle can strike the bottom of the insert without being damaged. The polymer feet act as a shock absorber. This allows all of the sample to be withdrawn. These inserts are self-aligning and stand straight.
Conical Inserts with Polymer Feet (100/Pk)* 100 L glass inserts with polymer feet and mandrel interior 100 L deactivated glass inserts with polymer feet and mandrel interior 100 L polypropylene inserts with polymer feet Part No. 5181-1270 5181-8872 5182-0549
*For use with Agilent Wide Opening Vials
P/N 5181-1270
Conical Glass Inserts

Conical inserts require no springs and are ready to drop into the vial for injections. The most economical choice for microvolume sampling.
Conical Glass Inserts (100/Pk)* 100 L pulled-point glass inserts Part No. 5183-2085
P/N 5183-2085
Flat Bottom Inserts

An economical limited-volume alternative. Manufactured with tight tolerances for a custom fit. The extremely flat bottom without any push-up protects syringe needles.
Flat Bottom Inserts (500/Pk)* 250 L glass flat bottom inserts 250 L deactivated glass flat bottom inserts 250 L polypropylene flat bottom inserts
P/N 5181-3377
Part No. 5181-3377 5183-2086 5183-2087

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Vials
8-425 Screw Top Vials
Agilent Standard Opening Screw Top Vials

The original, smaller opening vial with an 8-425 thread size screw cap. Available with black caps and a variety of septa to meet the needs of your application. Note: For microvolume sampling, see our Microvolume Inserts section.
Vial Highlights
8-425 thread design The original, narrow neck size Uniformly flat bottom ensures compatibility with inserts
Vials
8-425 Screw Top Glass Vials
These 2 mL, 12 x 32 mm 8-425 vials accommodate a variety of autosamplers originally designed for narrow neck vials. Use with 8-425 screw caps.
8-425 Screw Top Glass Vials (100/Pk) Clear 8-425 screw top, 2 mL Amber 8-425 screw top, 2 mL Part No. 5183-4428 5183-4429
P/N 5183-4428, 5183-4429
Deactivated 8-425 Screw Top Glass Vials

The same vials as described previously with the addition of a deactivation process to eliminate sample interactions. Ready-to-use for polar compound analysis such as phenols, proteins, antibodies, amines, metabolites, herbicides and pesticides. Use with 8-425 screw caps.
Deactivated 8-425 Screw Top Glass Vials (100/Pk) Clear deactivated 8-425 screw top, 2 mL Amber deactivated 8-425 screw top, 2 mL Part No. 5183-4432 5183-4433
Helpful Hint: Microvolume inserts are shown on page 31.
P/N 5183-4432, 5183-4433
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Vials
Caps and Septa
Caps
8-425 Screw Caps with Septa
Open top screw caps with the 8 mm septa installed. Note: See the Septum Selection Guide to choose the septum best suited for your application.
8-425 Screw Caps with Septa (100/Pk) Black 8-425 screw caps, red PTFE silicone septa Part No. 5183-4442
8-425 Screw Caps (no septa)
P/N 5183-4442, 5183-4438
Open top polypropylene screw caps without septa. Recommended instead of phenolic caps. Phenolic is a sulfur-based product that can flake and contaminate samples when syringe needle pushes through septum.
8-425 Screw Caps no Septa (100/Pk) Black 8-425 screw caps Part No. 5183-4438
Septa
Septa for 8-425 Screw Top Vials
Septa for 8-425 Screw Top Vials (100/Pk) Red PTFE silicone/red PTFE septa, 8 mm
Septa for 8-425 Screw Top Vials
Part No. 5183-4436 5183-4437
Red PTFE silicone septa, 8 mm
30
Vials
Inserts for 8-425 Vials/LC Vials
Inserts for 2 mL, 12 x 32 mm 8-425 Screw Top Vials

To meet your microsampling needs, Agilent has a variety of microvolume inserts with capacities and designs that can transform our vials from full-capacity to limited volume with one simple step. The inserts featured here are compatible with 8-425 screw top vials. (Not for use with wide opening vials.)
Conical Glass Inserts with Polymer Feet

Polymer feet act as the inserts' shock absorber to allow the syringe needle to strike the bottom without being damaged. This insert is self-aligning and stands straight.
Conical Glass Inserts with Polymer Feet (100/Pk) 100 L glass inserts with polymer feet. For 8-425 vials. Part No. 5183-2088
P/N 5183-2088
Conical Glass Inserts

Ready-to-use and require no springs. The most economical for microvolume sampling.
Conical Glass Inserts (100/Pk) 100 L pulled-point interior glass inserts for 8-425 vials Part No. 5183-2089
P/N 5183-2089
Flat Bottom Inserts

An economical limited-volume alternative. Manufactured with tight tolerances for a custom fit. The extremely flat bottom without any push-up protects syringe needles.
100 L Flat Bottom Inserts (100/Pk) 100 L glass flat bottom inserts for 8-425 vials Part No. 5183-2090
P/N 5183-2090
Vials for Agilent Technologies Liquid Chromatographs

Agilent Technologies offers a variety of vials for Agilent's LC instruments. Choose from small volume vials, 2 mL, and 6 mL vials. Agilent minivials are designed to allow for reproducible injections of minute quantities, making your limited sample applications more effective and more accurate. Our microvials provide limited volumes for microsampling and the 6 mL capacity vial allows for larger sample injections. For a complete selection of vials, caps and septa for LC, refer to the LC section of this catalog.
31
Vials
4 mL Vials
Agilent 4 mL Screw Top Vials, Caps and Septa

4 mL, 15 x 45 mm screw top glass vials are a custom fit to Agilent 6850 autosampler tray (G2880A) for use as sample as well as wash vials. Vials with fill marks are also appropriate for use as wash vials and waste vials in 7673 and 7683 autosamplers. These vials can also be used in Perkin-Elmer Autosystem GC, Waters 48 position tray autosampler, Shimadzu AOC-14/1400, LC-10A and the SIL-2AS. Use the 13 x 425 caps with septa for use as sample vials and with diffusion caps for wash vials. These vials can also be used as storage vials. Vial Highlights 4 mL, 15 x 45 mm Precision formed screw threads (13 x 425) for consistently secure seal Custom fit for 7673, 7683 and 6850 autosamplers Can be used as wash, waste or storage vials
P/N 5183-4448, 5183-4450
Agilent 4 mL Screw Top Vials (100/Pk) Clear screw top, 4 mL, 15 x 45 vials Amber screw top, 4 mL, 15 x 45 vials Agilent Caps and Septa for 4 mL Screw Top Vials Black 13 x 425 caps, PTFE/silicone septa Black open top screw caps Diffusion caps PTFE/natural rubber septa Red PTFE/white silicone septa White virgin PTFE septa Agilent 4 mL Vials, Caps and Septa Kits Clear screw top wash vials with screw caps (no septa) 4 mL wash vials with fill markings and caps Unit 100/pk 100/pk 12/pk 144/pk 100/pk 1000/pk Unit 144/pk 25/pk
Part No. 5183-4448 5183-4450 Part No. 5183-4464 5183-4461 07673-40180 9301-1031 5183-4460 5183-4459 Part No. 9301-0723 5182-0551
P/N 5183-4464, 07673-40180, 5183-4464
P/N 9301-0723, 5182-0551
32
Vials
Headspace Vials
Agilent Headspace Crimp Top Vials

These beveled neck vials are available in both 10 mL and 20 mL capacities, flat or rounded bottom. The 20 mm crimp caps provide a consistently secure seal. You can save when you purchase a pack quantitywith vial, cap and septa packaged together in a single container.
Vial Highlights
Beveled top for maximum secure seal Two neck lengths available Choice of a pressure safety release cap at 45 psi Available in flat or rounded bottom designs
Helpful Hint: For additional Headspace supplies see the GC Instruments Parts and Supplies section in this catalog.
Vials
Flat Bottom Headspace Crimp Top Glass Vials
20 mm beveled crimp top creates a much tighter seal than flat crimp surface. The slightly longer neck is custom fit for Agilent Technologies and Carlo Erba. Manufactured to exacting tolerances. Use with 20 mm crimp caps.
Flat Bottom Headspace Crimp Top Glass Vials (100/Pk) Flat bottom Headspace, 20 mL, 23 x 75 mm Flat bottom Headspace, 10 mL, 23 x 46 mm Part No. 5182-0837 5182-0838
P/N 5182-0837, 5182-0838
Rounded Bottom Headspace Crimp Top Glass Vials

20 mm beveled crimp top creates a much tighter seal than flat crimp surface. Designed for Perkin-Elmer. Also use with Tekmar and Varian. Heat annealed to 530C. Use with 20 mm crimp caps.
Rounded Bottom Headspace Vials (100/Pk) Rounded bottom Headspace, 20 mL, 23 x 75 mm Rounded bottom Headspace, 10 mL, 23 x 46 mm Part No. 5183-4474 5183-4475
P/N 5183-4474, 5183-4475
33
Vials
Caps
Caps
20 mm Crimp Caps Only
For use with all Headspace crimp top vials. Caps with safety feature release pressure at approximately 45 psi. Note: Review the Septum Selection Guide to choose the septum best suited for your application.
20 mm Crimp Caps Without Septa (100/Pk)
P/N 9301-0721, 9301-0718
Part No. 9301-0721
Silver aluminum, one piece crimp caps, 20 mm
Silver aluminum, one piece crimp caps with safety feature, 20 mm 9301-0718
20 mm Crimp Caps with Septa

Pre-assembled 20 mm crimp caps with septa. Ready-to-use with any Headspace crimp top vials.
20 mm Crimp Caps with Septa (100/Pk)
P/N 5183-4477, 5183-4479
Part No. 5183-4478 5183-4479 5183-4480
Silver aluminum, one piece crimp caps, 20 mm PTFE/silicone septa 5183-4477 Silver aluminum, one piece crimp caps with safety feature, 20 mm PTFE/silicone septa Silver aluminum, one piece crimp caps, 20 mm molded PTFE/butyl septa Silver aluminum, one piece crimp caps with safety feature, 20 mm molded PTFE/butyl septa
34
Vials
Septa/Vial Kits/Storage Vials
Septa
20 mm Septa
20 mm Septa (100/Pk) Gray butyl stopper, 20 mm Gray PTFE/black butyl molded septa, 20 mm Tan PTFE/white silicone septa, 20 mm Specs -40C to 120C -40C to 125C -60C up to 180C Part No. 5183-4476 9301-0976 9301-0719
P/N 5183-4476, 9301-0719, 9301-0976
Vial Kits
Supply Kit
Supply Kit (100/Pk) 20 mL flat bottom Headspace crimp top vials, silver aluminum one-piece crimp caps with safety feature, molded PTFE/Butyl Septa 20 mL flat bottom Headspace crimp top vials, silver aluminum one-piece crimp caps with safety feature, molded PTFE/Silicone Septa Specs <120C Part No. 5182-0839
>125C
5182-0840
Supply Kits
General Purpose Sample and Storage Vials

With six vial capacities available in clear or amber glass, Agilent vials are ideal for sampling or storage. Our Teflon-lined storage caps offer broad chemical resistancethe ultimate in glass closures. The polypropylene screw caps with Teflon/silicone septa are extra clean to eliminate sample contamination.
Vial Kits
Clear Glass Sample Vials with Teflon-Lined Storage Caps
Packaged as 100 pack of shrink-wrapped clear glass sample vials, with Teflon-lined solid-top storage caps in resealable plastic bagsall in our handy container to keep items dust-free.
Vials with Solid Storage Caps 4 mL, 15 x 45-vials with 13-425 caps 12 mL, 19 x 65-vials with 15-425 caps 22 mL, 23 x 85-vials with 20-400 caps 40 mL, 28 x 95-vials with 24-400 caps Unit 100/Pk 200/Pk 200/Pk 100/Pk Part No. 5183-4519 5183-4521 5183-4523 5183-4524
35
Vials
Storage Vials Amber Glass Sample Vials with Teflon-lined Storage Caps
Packaged as 100 pack of shrink-wrapped amber glass sample vials, with Teflon-lined solidtop storage caps in resealable plastic bags all in our handy container to keep items dust-free.
Amber Vials with Solid Storage Caps 4 mL, 15 x 45-vials with 13-425 caps 12 mL, 19 x 65-vials with 15-425 caps 40 mL, 28 x 95-vials with 24-400 caps Unit 100/pk 200/pk 200/pk Part No. 5183-4526 5183-4528 5183-4530
Clear Glass Sample Vials with Caps and Septa
Vial packs are shrink-wrapped in quantities of 100 or 200. Natural colored Teflon/clear silicone septa and polypropylene open top caps are each in resealable plastic bags. All items are packed in our handy container to keep items dust-free.
Clear Vials with Caps and Septa 4 mL, 15 x 45-vials with 13-425 caps 12 mL, 19 x 65-vials with 15-425 caps 22 mL, 23 x 85 vials with 20-400 caps 40 mL, 28 x 95-vials with 24-400 caps Unit 100/pk 200/pk 200/pk 100/pk Part No. 5183-4532 5183-4534 5183-4536 5183-4537
36
Vials
Electronic Crimpers and Decappers
Agilent Crimping and Decapping Tools

Electronic Crimpers and Decappers
Is your hand getting tired from crimping or decapping too many vials? Should your crimp seals be more consistent vial to vial? Try the new Electronic Crimpers and Decappers from Agilent Technologies. They crimp or decap vials easily and consistently with the push of a button. These devices, with battery-powered motors, will make crimping and decapping vials a breeze and even fun. The units use standard power tool batteries and allow you to crimp and decap hundreds of vials before you have to recharge the battery. You can adjust the crimp or decap force to your specifications.
Electronic Crimpers and Decappers Electronic Crimper: 11 mm, complete with one VersaPak Gold rechargeable battery and charger. Recommended for 2 mL, 12 x 32 mm crimp top autosampler vials. Part No. 5183-4763
Electronic Decapper P/N 5184-3567, 5184-3573
Electronic Crimper P/N 5183-4763, 5184-3572
Electronic Decapper: 11 mm, complete with one VersaPak Gold 5184-3567 rechargeable battery and charger. Recommended for 2 mL, 12 x 32 mm crimp top autosampler vials. Electronic Crimper: 20 mm, complete with one VersaPak Gold rechargeable battery and charger. Recommended for 20 mm headspace or serum vials. 5184-3572
Electronic Decapper: 20 mm, complete with one VersaPak Gold 5184-3573 rechargeable battery and charger. Recommended for 20 mm headspace or serum vials. Black & Decker VersaPak Gold NiMH rechargeable battery 5183-4799
Black & Decker Rechargeable Battery P/N 5183-4799
37
Vials
Manual Crimpers and Decappers/Vial Racks
Manual Crimpers and Decappers

8 mm Crimping and Decapping Tools
Use with all 8 mm crimp top vials and crimp caps.
Manual Crimper and Decapper
Manual Crimping and Decapping Tools Manual crimper, 8 mm Manual decapper, 8 mm Manual crimper, 11 mm Manual decapper, 11 mm Manual crimper, 20 mm Manual decapper, 20 mm Crimping System and Crimp Heads Holder without crimp head, to be mounted Crimp head, 11 mm Crimp head, 20 mm Decapper head, 11 mm Decapper head, 20 mm
Part No. 8710-1643 5181-8827 8710-0979 5181-1213 9301-0720 5181-1214 Part No. 5062-2495 5001-3755 5001-3756 5001-3758 5001-3759
Vial Racks
Vial Racks
P/N 9301-0722
Part No. 5061-3349 5062-2405 9301-0722
Rack for 8 mm, 100 and 300 L minivials, solid plexiglass Rack for 8 mm, 500 and 700 L minivials, solid plexiglass Rack for 12 mm, microvials, blue plastic
P/N 5061-3349
38
Syringes
General Information
Syringes
With a broad selection of syringes for manual and auto injection, Agilent has what you need for accurate and effective sampling.
Typical Needle Gauge Dimensions

OD Gauge 22 23s 25 26s mm 0.71 0.635 0.5 0.47 inches 0.028 0.025 0.02 0.185 mm 0.41 0.11 0.2 0.11 ID inches 0.016 0.0045 0.008 0.0045
Needle and Point Styles

Needle gauge, length, and point styles are referenced in syringe listings as gauge/length in mm/point style (e.g., 26s/42/HP).
Needle Styles
Fixed Needle Syringe: The epoxy-cemented, stainless steel needle is centered in the syringe barrel at the depth of the zero graduation mark. Do not heat above 50C. Removable Needle Syringe: Removable needle allows use of various point styles. The threaded hub connection and Teflon sealing ferrule can be tightened to compensate for wear.
Point Styles
HP Point Style: This proprietary design yields optimal performance and reliability and reduces coring by parting, not cutting, the septum. Cone Tip: On Perkin-Elmer and Shimadzu syringes.
Cone Tip HP Point
Point Style 2: A standard, general purpose point style. Needle has a 20 bevel.
Point Style 2
Point Style 3: For manual LC injectors. The needle has a 90 bevel, ideal for a volume displacement syringe. Side Hole Point Style 5: Syringe needle has a side hole for sample filling and dispensing.
Point Style 3
Side Hole/Point Style 5
Note: For more detailed information on syringe selection, ask for pub. no. 5968-5283E.
39
Syringes
Autosampler Syringes
Agilent Autosampler Syringes

Tapered Needle, 23-26s Gauge Autosampler Syringes
Use one needle and get the benefits of two. The upper portion of the tapered needle offers the strength of a 23 gauge, while the lower portion at 26s gauge allows for use with split/splitless or on-column injections with 0.53 mm id columns. All standard plungers are stainless steel.
Volume (L)
Tapered Needle Syringes
Unit
Needle
Part No.
Fixed needle Fixed needle Removable needle 6/pk 3/pk 6/pk 3/pk 6/pk
23-26s/42/HP 23-26s/42/HP 23-26s/42/HP 23-26s/42/HP 23-26s/42/HP 23-26s/42/HP 23-26s/42/HP 23-26s/42/HP 23-26s/42/HP 23-26s/42/HP 23-26s/42/HP 23-26s/42/HP 23-26s/42/HP
5181-1273 5181-8810 5182-0835 5182-0832 5181-1267 5181-3360 5181-3321 5181-3319 5181-3354 5181-3361 5181-3365 5181-3356 5181-3358 5183-0314 5183-2042
Replacement needle for 5 L syringe 10 Fixed needle Fixed needle Removable needle Replacement needle for 10 L syringe Fixed needle, Teflon-tipped plunger Fixed needle, Teflon-tipped plunger Removable needle, Teflon-tipped plunger
Replacement plunger with Teflon tip for fixed needle 10 L syringe Replacement plunger with Teflon tip for removable needle 10 L syringe 50 100 Fixed needle, Teflon-tipped plunger Fixed needle, standard plunger
Helpful Hint: Teflon-tipped plungers are gas tight
Fixed Needle
Removable Needle
40
Syringes
Autosampler Syringes Straight Needle, 23s and 26s Gauge Autosampler Syringes
Volume (L) Straight Needle Syringes Unit Needle Part No.
Fixed needle, 26 gauge Fixed needle, 26 gauge Fixed needle, 23 gauge Fixed needle, 23 gauge Removable needle, 23 gauge Replacement needle for 5 L syringe 3/pk 6/pk 6/pk 6/pk 6/pk 3/pk 6/pk 6/pk
26s/42/HP 26s/42/HP 23s/42/HP 23s/42/HP 23s/42/HP 23s/42/HP 26s/42/HP 26s/42/HP 23s/42/HP 23s/42/HP 23s/42/HP 23s/42/HP 23s/42/HP 23s/42/HP 23s/42/HP 23s/42/HP 23s/42/HP 23s/42/HP
9301-0891 5183-4728 9301-0892 5182-0875 5182-0834 5182-0830 9301-0714 5183-4729
10
Fixed needle, 26 gauge Fixed needle, 26 gauge Fixed needle, 23 gauge Fixed needle, 23 gauge Fixed needle, Teflon-tipped plunger Fixed needle, Teflon-tipped plunger Replacement plunger for 10 L fixed needle syringe Removable needle, 23 gauge Removable needle, Teflon-tipped plunger Replacement needle for 10 L syringe Replacement plunger for 10 L removable needle syringe
General Cmatography
9301-0713 9301-0725 5181-8809 5183-4730 5181-8808 5181-8806 5181-8813 5181-8811 5181-3358 5183-0316 5183-0318 5183-2058
25 50 100
Fixed needle, Teflon-tipped plunger Fixed needle, Teflon-tipped plunger Fixed needle, standard plunger
Autosampler Syringes 6-pack
41
Syringes
Autosampler Syringes Agilent 7673/7683 On-Column Syringes
Agilent Technologies 7673/7683 on-column syringes with needle diameters for columns ranging from 0.25 mm to 0.53 mm. Specifically designed for the 7673/7683 Autosampler it's another Perfect Fit.
Volume (L) 5
Removable Needle
7673/7683 On-Column Syringes Removable needle, barrel only SS needle for 0.53 mm column SS steel needle for 0.32 mm column SS steel needle for 0.25 mm column Plunger button
Unit 3/pk 3/pk 3/pk 10/pk
Part No. 5182-0836 5182-0832 5182-0831 5182-0833 5181-8866
HP 7670/71/72 Syringes
This syringe has a long needle and regular plunger button for compatibility with HP 7670/71/72 autosamplers. Available with a fixed or removable needle.
Volume (L) 1 10 10 10
7670/71/72 Syringes Removable needle Fixed needle Removable needle Fixed needle, Teflon-tipped plunger Replacement plunger for 10 L syringe
Unit
Needle 23s/56/2
Part No. 5182-9622
23s/50/HP 5182-9734 23s/50/HP 5182-9626 23s/50/HP 5182-9799 5182-9800
42
Syringes
Manual Syringes
Manual Syringes
Manual, Low Volume Plunger-in-Needle Syringes
Removable needle syringes with capacities from 0.5 L to 5.0 L. The plunger is in the needle and the sample is contained within the needle.
Volume (L) 0.5 1.0 1.0 5.0
Plunger In Needle Syringes Removable needle Removable needle Replacement needle/plunger for 1.0 L syringe Removable needle Replacement needle/plunger for 1.0 L syringe Removable needle Replacement needle/plunger for 5.0 L syringe
Needle 23s/70/HP 26s/70/HP 26s/70/HP 23s/70/HP 23s/70/HP 23s/70/HP 23s/70/HP
Part No. 5182-9651 5182-9605 5182-9635 5182-9627
5182-9712 5182-9623 5182-9683
Manual, Regular Plunger Syringes

Volume (L) 10 Regular Plunger Syringes Fixed needle with plunger protection Fixed needle 25 50 100 250 500 Fixed needle Fixed needle Fixed needle Fixed needle Fixed needle 10/pk Unit Needle 26s/50/2 26s/50/2 25/50/2 25/50/2 25/50/2 25/50/2 25/50/2 Part No. 5182-3499 5182-3428 5182-9625 5182-9636 5182-9615 5182-9640 5182-9641
25-500 L Fixed Needle 10 L Fixed Needle
43
Syringes
Manual Syringes Manual Syringes with Teflon-Tipped Plunger (Gas-Tight)
Available with a fixed needle, these syringes are for general purpose injections. With gastight construction, they are ideal for sampling gases and volatile materials.
Volume Gas-Tight Manual Syringes 10 L 25 L 50 L Fixed needle, Teflon-tipped plunger Replacement plunger with Teflon tip for 10 L syringe Fixed needle, Teflon-tipped plunger Fixed needle, Teflon-tipped plunger 25s/50/2 25s/50/2 25s/50/2 25s/50/2 25/50/2 23/50/2 23/50/2 23/50/2 23/50/2 Needle 26s/50/2 Part No. 5182-9606 5182-9621 5182-9629 5182-9612 5182-9610 5182-9608 5182-9609 5182-9604 5182-9661 5182-9642 5182-9671 5182-9638 5182-9643 5182-9709
100 L Fixed needle, Teflon-tipped plunger 250 L Fixed needle, Teflon-tipped plunger
Fixed Needle
500 L Fixed needle, Teflon-tipped plunger 1.0 mL Fixed needle, Teflon-tipped plunger Replacement plunger with Teflon tip for 1.0 mL syringe 2.5 mL Fixed needle, Teflon-tipped plunger Replacement plunger with Teflon tip for 2.5 mL syringe 5.0 mL Fixed needle, Teflon-tipped plunger 10 mL Fixed needle, Teflon-tipped plunger Replacement plunger with Teflon tip for 10 mL syringe
SuperfleX Plunger Syringes (not available in Japan)

The super elastic alloy plunger, together with the plunger protection guide and button reinforcement, ensure precise and smooth injections. The plunger is flexible and returns to its original shape after injection.
Volume (L) 5
Superflex Syringe
Superflex Plunger Syringes Removable needle, SuperfleX syringe Fixed needle, SuperfleX syringe (6/pk) Fixed needle, SuperfleX syringe
Needle 26s/50/2 26s/50/2 26s/50/2
Part No. 5182-3498 5182-3495 5182-3496
10 10
44
Syringes
Manual Syringes On-Column Syringes
Ideal for manual on-column injections. Depending on column diameter, choose either fused silica or durable stainless steel.
Vol. (L) Manual On-Column Syringes 10 Removable needle, stainless steel Replacement needle for 10 L syringe 3/pk 10 Removable needle, fused silica (syringe only) Fused Silica needle for 10 L syringe 6/pk Replacement Teflon ferrule for P/N 9301-0658
Unit
Needle 0.23 mm /10 cm 0.23 mm /10 cm
Part No. 5182-9633 5182-9645 9301-0658

On-Column Syringe
0.18 mm/11.5 cm 19091-63000 0100-1389
Gas-Tight Syringes with Luer Lock Valve

Offer pressure tight sample containment up to 100 psi for liquid and gas samples.
Volume (mL) 1 2.5 5 10 25 50
Gas-Tight Syringes w/Luer Lock Valve Gas-tight syringe, fitted with push-pull luer lock valve Gas-tight syringe, fitted with push-pull luer lock valve Gas-tight syringe, fitted with push-button luer lock valve Gas-tight syringe, fitted with push-button luer lock valve Gas-tight syringe, fitted with push-button luer lock valve Gas-tight syringe, fitted with push-button luer lock valve
Part No. 5183-4549 5183-4550 5183-4551 5183-4552 5183-4553 5183-4554

Push Button Push Pull
45
Syringes
Manual Syringes Gas-Tight Syringes with Luer Lock
With the Teflon luer lock syringe your sample comes in contact with glass and Teflon reducing the risk of contamination. Use with syringe filters and for pump priming in LC applications.
Teflon Leur Lock
Volume (mL) 1.0 2.5 5.0
Gas-Tight Syringes w/Luer Lock Teflon luer lock Replacement plunger for 1.0 mL syringe Teflon luer lock Replacement plunger for 2.5 mL syringe Teflon luer lock Teflon luer lock Replacement plunger for 10 mL syringe Teflon luer lock
Part No. 5182-9710 5182-9661 5182-9711 5182-9671 5182-9630 5182-9620 5182-9709 5182-9639
10 25
Helpful Hint: Use this product with syringe filters.
Luer Lock Needles

Luer Lock Needles
Luer Lock Needle
Unit 5/pk 2/pk 2/pk
Needle 23/50/2 23/50/5 22/51/3
Part No. 5183-4606 5183-4613 5183-4614
Needle, luer lock Needle, luer lock Needle, luer lock
LC Injector Valve Syringes

Manual Injector Valve Syringes, Rheodyne, Altex, Valco Injector Syringes
Use with a variety of liquid chromatographs including the Rheodyne, Altex and Valco sampling valves. The blunt-tip point style (3) prevents tearing of the LC injector valve needle seats.
Volume (mL) 10
Fixed Needle
Manual Injection Valve Syringes Fixed needle Fixed needle Fixed needle Fixed needle Fixed needle Fixed needle Removable needle
Needle 22/51/3 22/51/3 22/51/3 22/51/3 22/51/3 22/51/3 22/50/3
Part No. 5182-9644 5182-9628 5182-9619 5182-9613 5182-9624 5182-9658 5183-4547
25 50 100 250 500 500
46
Gas Management
Gas Purifiers
Gas Purifiers
Agilent Technologies brings the highest performance and largest variety of gas purifiers to gas chromatographers. We manufacture purifiers in an astonishing variety of sizes and configurations, to remove oxygen, moisture, and hydrocarbons. Our product line contains in-line gas purifiers including refillable, indicating, S-shaped, and metal body types. In line gas purifiers are made to remove specific contaminants. We also carry gas purification systems with removable cartridges. These systems provide the ability to design the right combination of filters needed for your application. Please refer to the following Gas Purifier Selection Guide to determine which gas purifiers you should use.
47
Gas Management
Gas Purifiers Gas Purifier Selection Guide
In Line Purifiers
Contaminant Removed Description Series Unique Features Refillable Page No.
Moisture(H20)
Economy Indicating Moisture Traps Glass Indicating Moisture Traps S-Traps Big Moisture Traps Refills for Moisture Traps
MT, MT-D, MT-S GMT, LGMT
Economical plastic Lexan body for durability Glass body for no moisture diffusion Can be reconditioned in GC oven Ultra-high capacity for moisture Makes gas purification more economical Glass construction with plastic safety shield Economical non-indicating oxygen trap Ultra-high capacity for oxygen Economical trap for hydrocarbons Ultra-high capacity for hydrocarbons Can be reconditioned in GC oven High surface area adsorbent, purged with high purity helium Makes gas purification more economical Bi-functional trap, leak-free onepiece design Bi-functional trap, leak-free onepiece design Gas-specific (for purifying He, N2, or H2), ultra-high capacity, removes H2O, O2, HCs, CO, and CO2, recommended for GC and GC/MS gas supplies Makes gas purification more economical
Yes Yes No Yes
5153 54 55 55 55
BMT MSR-1, MSR-2, SGR, GMSR, BMSR-1 IOT, LIOT OT1 BOT HT BHT HT3 ACR, BACR OT3 HMT RMS
Oxygen (02)
Indicating Oxygen Traps Oxygen Traps Big Oxygen Trap
No No No Yes Yes No Yes
56 56 57 57 58 58 58 5758
Hydrocarbons (HCS)
Hydrocarbon Traps Big Hydrocarbon Trap S-Trap Capillary Grade Hydrocarbon Trap Refills for Hydrocarbon Traps
Combination Traps
Oxygen/Moisture Trap Hydrocarbon/Moisture Trap Big Universal Trap
No Yes No
6061 61 62
Refill for Hydrocarbon/Moisture Trap HCRMS
61
Purification Systems
Contaminants Description Unique Features Refillable Page No.
Multiple
High Capacity Gas Purification System
1 or 3 position cartridge system with ultra-high capacity and rugged, allwelded stainless steel construction for optimal purity, available with triple combination trap 1,2,3, or 4 position cartridge system with quick change (QC) connections and unique valves to change cartridges without interrupting gas flow to the instrument 1,2,3, or 4 position modular, quickconnect cartridge system with visible indicators
No
6364
Quick Change + Point of Operation Panel
No
6567
Super-Clean Gas Filter System
No
6869
48
Gas Management
Gas Purifiers Gas Purifier Selection Guide (continued)
Other
Contaminant Removed Description Series Unique Features Refillable Page No.
Organics
Split Vent Trap
RDT
Cartridge design with durable cartridge holder and replacement cartridges
No
70
Accessories
Description Series Unique Features Page No.
Mounting Clips Trap Mounting Panel
MC, UMC UMP
Designed to hold In-Line Purifiers Designed to hold In-Line Purifiers
70 70
Carrier Gas Purification
49
Gas Management
Oxygen and Moisture Trap Capacities
Oxygen Removal
Type of Trap OT3 series IOT series LIOT series OT3 + IOT GC-1 GC-4 RBC series RQC series BOT series Removal Capacity (mg) 600.0 30.0 100.0 630.0 396.0 40.0 436.0 436.0 3,400.0 Performance (ppb) 15 2 1 2 5 2 2 2 1
Moisture Removal
Type of Trap MT120 series MT120-D series MT120-S series MT200 series MT200-D series MT200-S series MT400 series MT400-D series MT400-S series GMT series GMT-GC series GC-2 GC-2-I BMT-series Removal Capacity (g) 21.6 21.6 31.5 36.0 36.0 52.5 72.0 72.0 105.0 16.3 11.4 15.0 7.0 130.0 Performance (ppb) 20 22 40,000 18 20 39,000 14 16 39,000 6 7 9 9 5
Performance numbers are the lowest levels observed using N2 @ 1 L/min.
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Gas Management
Moisture Traps
Moisture Removal Traps

Economy Indicating Refillable Moisture Traps
Agilent Indicating Moisture Traps are designed to remove water, oil, and organics from gases employed in, but not limited to, gas chromatography. Refillable moisture traps are constructed from Lexan brand polycarbonate tubing. Lightweight, chemically resistant and of superior strength when compared to traps manufactured from acrylic. Agilent Indicating Moisture Traps have become the chromatographers choice for water adsorption from GC gases. Available in three refillable sizes and three different packings Both inlet and outlet connectors are equipped with stainless steel frits to prevent particulates from entering the gas stream Mixed packing bed makes improper installation impossible Easily refillable Tested to 150 psi (helium)
Refillable Moisture Trap MT120
Molecular Sieve 13X and Indicating 4

The preferred adsorbent for GC gas drying. Blue indicating sieve turns buff at 20% relative humidity.
Molecular Sieve 13X (MT Series)

Maximum Effluent H2O Removal Concentration Fitting (in.) Capacity (g) (ppb) Part No.
Description
Size (cc)
Refillable Moisture Trap Refillable Moisture Trap Refillable Moisture Trap Refillable Moisture Trap Refillable Moisture Trap Refillable Moisture Trap Adsorbent Refill (1 pint) for MT Series Mounting Clip for 120 & 200 cc traps Universal Mounting Clip for 400 cc traps
120 120 200 200 400 400
1/8 1/4 1/8 1/4 1/8 1/4
21.6 21.6 36.0 36.0 72.0 72.0
20 20 18 18 14 14
MT120-2 MT120-4 MT200-2 MT200-4 MT400-2 MT400-4 MSR-1
MC-1
UMC-4
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Gas Management
Moisture Traps
Molecular Sieve 5 and Indicating Drierite
High moisture capacity and simultaneously removes hydrogen sulfide and oil. Indicating Drierite changes dramatically from bright blue to pink as the gas stream approaches 40% relative humidity.
Molecular Sieve 5 (MT-D Series)

Description
Size (cc)
Refillable Moisture Trap
120 120 200 200 400 400
1/8 1/4 1/8 1/4 1/8 1/4
21.6 21.6 36.0 36.0 72.0 72.0
22 22 20 20 16 16
MT120-2-D MT120-4-D MT200-2-D MT200-4-D MT400-2-D MT400-4-D MSR-2
Refillable Moisture Trap Refillable Moisture Trap Refillable Moisture Trap Refillable Moisture Trap Refillable Moisture Trap Adsorbent Refill (1 pint) for MT-D Series
Mounting Clip for 120 & 200 cc traps Universal Mounting Clip for 400 cc traps
MC-1 UMC-4
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Gas Management
Moisture Traps
Silica Gel, Grade 40, and Indicating Silica Gel, Grade 42
Highest moisture capacity adsorbent. Adsorbs as much as 40% of its weight in water. High affinity for hydrocarbons. Blue indicating gel turns from a deep blue to pale pink at 40% relative humidity.
Silica Gel 40 (MT-S Series)

Description
Size (cc)
Refillable Moisture Trap Refillable Moisture Trap Refillable Moisture Trap Refillable Moisture Trap Refillable Moisture Trap Refillable Moisture Trap Adsorbent Refill (1 pint) for MT-S Series
Refillable Moisture Trap MT400
120 120 200 200 400 400
1/8 1/4 1/8 1/4 1/8 1/4
31.5 31.5 52.5 52.5 105.0 105.0
40 40 39 39 39 39
MT120-2-S
MT120-4-S MT200-2-S MT200-4-S MT400-2-S MT400-4-S SGR
Mounting Clip for 120 & 200 cc traps Universal Mounting Clip for 400 cc traps
MC-1 UMC-4
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Gas Management
Moisture Traps Glass Indicating Moisture Traps
Ideal for GC/MS Systems, Electron Capture Detectors, and Electrolytic Conductivity Detectors
Outlet Gas has less than 1% inlet concentration Polishing Zone Active Desiccant
Molecular Sieve 13X and a band of Indicating Molecular Sieve 4 Available in 70 cc, 100 cc and 250 cc sizes Heavy wall borosilicate glass tube no moisture diffusion Refillable
Capacity and Efficiency
Mass Transfer Zone (MTZ) Gas at this point has less than 5% of inlet concentration Scrubber Zone
The 100 cc unit will treat 10 standard A cylinders with up to 30 ppm water or 16.3 grams to less than 10 ppb. Molecular sieves differ from all other commercially available adsorbents, as they have an extremely high adsorption capacity for water and polar compounds even at relatively low concentrations. The color change takes place in the middle of the trap bed, giving ample warning. In addition to the inherent qualities of the sieve material, we treat the bed material under high vacuum and heat to ensure maximum scrubbing efficiency and capacity.
Glass Indicating Moisture Traps (GMT and LGMT Series)

Maximum Moisture Effluent H2O Removal Concentration Size (cc) Fitting (in.) Capacity (g) (ppb) Part No.
Description
Desiccant is at equilibrium with inlet gas adsorbat concentration
Glass Indicating Moisture Trap Glass Indicating Moisture Trap
70 70 100 100 250 250
1/8 1/4 1/8 1/4 1/8 1/4
11.4 11.4 16.3 16.3 40.09 40.09
7 7 6 6 6 6
GMT-2GC-HP GMT-4GC-HP GMT-2-HP GMT-4-HP LGMT-2-HP LGMT-4-HP GMSR
Inlet Gas
Glass Indicating Moisture Trap Glass Indicating Moisture Trap Glass Indicating Moisture Trap Glass Indicating Moisture Trap
Refillable Glass Moisture Trap
Molecular Sieve 250 Refill for GMT and LGMT Series Mounting Clip for 70 & 100 cc traps Mounting Clip for 250 cc traps
UMC-3
UMC-4
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Gas Management
Moisture Traps Moisture Removal S-Traps
Contains Molecular Sieve 5, 45/60 mesh Can be reconditioned
Moisture Removal S-Traps

Description Moisture S trap,* preconditioned. Moisture trap: metal casing, S-shaped traps for carrier gas cleanup, 1/8 in. fittings Moisture S trap, not conditioned, 1/8 in. fittings Part No. 5060-9084
Moisture S-Trap
5060-9077
*These traps can be reconditioned by heating at a minimum of 350C, with flow for 6 hours.
Helpful Hint: Purifier log books are useful for determining maintenance schedules.
Big Moisture Traps

Capacity: 130 grams H2O Pressure: Up to 250 psig Efficiency: reduction of H2O to less than 5 ppb 750 cubic centimeter gas purifiers Ideal for bulk purification applications or where several instruments are plumbed from a single gas source One piece of heavy-walled aluminum tube. This one-piece design eliminates potential leaks. Equipped with sintered stainless steel frits to prevent particulate contamination Refillable
Big Moisture Traps (BMT Series)

Description Big Moisture Trap Big Moisture Trap Refill for Big Moisture Trap (enough for 2 refills) Big Mounting Clip, 2/pk Size (cc) Fitting (in.) Part No. 750 750 1/8 1/4 BMT-2 BMT-4 BMSR-1 UMC-5-2
Moisture Trap Refills

Description Adsorbent Refill (1 pint) Adsorbent Refill (1 pint) Adsorbent Refill (1 pint) Molecular Sieve Refill (250 cc) Use with MT series MT-D series MT-S series GMT & LGMT series Part No. MSR-1 MSR-2 SGR GMSR BMSR-1
Big Moisture Trap
Refills for Big Moisture Trap (enough for 2 refills) BMT series
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Gas Management
Oxygen Traps
Oxygen Removal Traps

Indicating Oxygen Traps
Not just an Indicator, but an Indicating Trap
Indicating Oxygen Trap
Reduces oxygen to less than 1 ppb Environmentally safe Available in two sizes, standard and largethree times the capacity This trap actually removes the oxygen rather than converting it to another form of contamination. Oxygen and a wide range of oxides react with the activated bed material to form a manganese oxide. This reaction results in a progressive and dramatic color change, from light green in the adsorbents active state to deep brown as oxygen saturation occurs. The Indicating Oxygen Trap is designed to be used in conjunction with any of our high capacity, non-indicating oxygen traps. When properly installed downstream from the non-indicating trap, the Indicating Oxygen Trap provides a visual indication of oxygen breakthrough BEFORE contamination reaches sensitive system components. Unlike adsorbent materials utilized in competitive products, our indicating media can be exposed to air and water in virtually any quantity without resulting in an exothermic reaction. The spent product is nontoxic, nonhazardous, nonflammable, and nonreactive. The high impact Lexan shield prevents lab catastrophes if the trap is exposed to pressure beyond its stated pressure limit. Safe for land-fill refuse.
Indicating Oxygen Traps (IOT and LIOT Series)

Description Indicating O2 Trap (IOT) Indicating O2 Trap Large Indicating O2 Trap (LIOT) Large Indicating O2 Trap Mounting Clip for IOT Trap Mounting Clip for LIOT Trap Size (cc) 30 30 150 150 Fitting (in.) 1/8 1/4 1/8 1/4 Part No. IOT-2-HP IOT-4-HP LIOT-2 LIOT-4 UMC-2 UMC-3
Economy Non-Indicating Oxygen Trap (OT1 Series)

Description Oxygen Trap Oxygen Trap Mounting Clip for OT1 Trap
Economy Non-Indicating Oxygen Trap
Size (cc) 70 70
Fitting (in.) 1/8 1/4
Part No. OT1-2 OT1-4 MC-1
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Gas Management
Oxygen Traps/Hydrocarbon Traps Big Oxygen Traps
Capacity: 3 liters O2 or 3,200 mg Pressure: Up to 250 psig Efficiency: reduction of O2 to less than 1 ppb 750 cubic centimeter gas purifiers Ideal for bulk purification applications or where several instruments are plumbed from a single gas source One piece of heavy-walled aluminum tube. This one-piece design eliminates potential leaks. Equipped with sintered stainless steel frits to prevent particulate contamination
Big Oxygen Trap
Big Oxygen Traps (BOT Series)

Description Big Oxygen Trap Big Oxygen Trap Big Mounting Clip, 2/pk Size 750 cc 750 cc Fitting (in.) 1/8 1/4 Part No. BOT-2 BOT-4 UMC-5-2
Hydrocarbon Removal Traps

Hydrocarbon Traps
Our Hydrocarbon Traps offer you flexibility: refill your trap and remove extremely low levels of hydrocarbons. Remove organics from carrier gases, air and hydrogen High capacity200 cc of filtering media Impregnated carbon filter media Mounting Panel on page 70 Refillable
Hydrocarbon Trap
Hydrocarbon Traps (HT Series)

Description Refillable Hydrocarbon Trap Refillable Hydrocarbon Trap Mounting Clip for HT200 Series Adsorbent Refill (1 pint) 2 Recharges for Hydrocarbon Trap Size 200 cc 200 cc Fitting (in.) 1/8 1/4 Part No. HT200-2 HT200-4 MC-1 ACR
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Gas Management
Hydrocarbon Traps Big Hydrocarbon Trap
Capacity: 80 grams of medium to heavy molecular weight hydrocarbons Pressure: Up to 250 psig Efficiency: reduction of C4 hydrocarbons to less than 15 ppb 750 cubic centimeter gas purifiers Ideal for bulk purification applications or where several instruments are plumbed from a single gas source One piece of heavy-walled aluminum tube. This one-piece design eliminates potential leaks. Equipped with sintered stainless steel frits to prevent particulate contamination Refillable
Big Hydrocarbon Trap
Big Hydrocarbon Traps
Description Big Hydrocarbon Trap Big Hydrocarbon Trap Refill for Big Hydrocarbon Trap (enough for two refills) Big Mounting Clip, 2/pk
Size 750 cc 750 cc
Part No. BHT-2 BHT-4 BACR UMC-5-2
Hydrocarbon Removal S-Trap

Filled with 40/60 mesh activated charcoal Can be reconditioned
Hydrocarbon Removal S-Trap

Description
Hydrocarbon S-Trap
Part No. 5060-9096
Hydrocarbon S Trap: S-shaped trap used for trapping organics from gases, 1/8-in. fittings
*This trap can be reconditioned by heating at a minimum of 350C, with flow for 6 hours.
Capillary Grade Hydrocarbon Traps

Extremely high surface area, coconut shell-based, activated carbon 100 cc of filtering media Purged with ultra-high purity helium Refillable
Capillary Grade Hydrocarbon Traps

Description Capillary Grade Hydrocarbon Trap Capillary Grade Hydrocarbon Trap Mounting Clip for HT3 Series Adsorbent Refill (1 pint) 3 Recharges for Capillary Grade Hydrocarbon Trap Size 100 cc 100 cc Fitting (in.) 1/8 1/4 Part No. HT3-2 HT3-4 MC-1 ACR
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Gas Management
Hydrocarbon Traps
Helpful Hint: Agilent Hydrocarbon Traps remove many types of compounds from gas streams.
Ability of Activated Carbon to Remove Substances from Gases Using Hydrocarbon Traps
Compound acetone hypochlorous acid alcohol amines inorganic acids ammonia iodine amyl acetate isopropyl acetate amyl alcohol isopropyl alcohol benzene ketones butyl acetate butyl alcohol lactic acids by-products-organic lysol calcium hypochlorite mercaptans carbon dioxide methyl acetate chlorobenzene methyl alcohol chlorine methyl bromide chlorophenol methyl chloride chlorophyl methyl ethyl ketone cresol Efficiency excellent excellent excellent very good none poor excellent excellent excellent excellent excellent excellent excellent excellent excellent excellent very good excellent excellent excellent none excellent excellent excellent excellent excellent excellent excellent excellent excellent excellent Compound dissolved oils nitrobenzenes nitrotoluene ethyl acetate ethyl alcohol organic acids ethyl chloride oxalic acid ethyl ether ozone fluoride phenol formaldehyde potassium permanganate propyl acetate glycol propyl alcohol propyl chloride hydrogen bromide hydrogen chloride sodium hypochlorite hydrogen fluoride solvents hydrogen iodide sulfuric acid hydrogen selenide hydrogen sulfide toluene trichloroethylene xylene Efficiency excellent excellent excellent excellent excellent excellent excellent excellent excellent excellent poor excellent poor excellent excellent excellent excellent excellent satisfactory poor excellent none excellent satisfactory satisfactory satisfactory satisfactory excellent excellent excellent
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Gas Management
Combination Traps
Combination Traps
Oxygen/Moisture Traps
Oxygen/moisture adsorbents team up to give you two functionalities in the same trap. Unlike some oxygen/moisture traps, these traps are disposable. Consider the safety, performance, and cost advantages of the Agilent capillary grade oxygen/ moisture traps (0T3) when comparing to heated, catalytic traps. Optimized for maximum surface area and capacity Leak-free, one-piece design(tested to 2000 psi) Bed material treated with ultra-high purity helium Filter design: prevents channeling, promotes efficient scrubbing
We recommend this trap for these applications:
Electron Capture Detectors Recommended by Agilent for electron capture detectors, oxygen/water removal effectively prevents detector degradation from oxygen and water contamination. The all-metal housing of the Agilent 0T3 trap virtually eliminates contamination and resultant signal noise. GC/MS
GC/MS Evaluation of the Agilent OT3 Trap - Heres the Proof:
1. Mass spectral data was acquired to determine background. 2. The trap was installed between the gas cylinder and the GC and data was acquired. Experimental Conditions A. High purity helium B. 80-100 scans of mass spectral data were acquired. Helium admitted to MS during scans 25-45. Ten scans were summed and scans 10-20 were used to correct for inherent instrument background. C. Agilent trap was installed and experiment was repeated. D. After additional use, the experiment was repeated. The data shows about a twentyfold reduction in the M/Z 18 ratio (water) and about a fortyfold reduction in the M/Z 32 ratio (oxygen).
M/Z Ratio* Without Agilent OT3 Trap With Agilent OT3 Trap
18/28 0.098 0.005
32/28 0.266 0.0118
32/40 11.08 0.247
*Contribution of inherent instrument background removed. Analysis by: California Analytical Laboratories, Inc. Sacramento, California
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Gas Management
Combination Traps
In addition to inert gases (nitrogen, helium, argon and krypton), the Agilent OT3 Trap treats streams of hydrogen, alkanes, alkenes, aliphatic hydrocarbon gases, low boiling aromatics, carbon dioxide, carbon monoxide, and argon-methane.
Why use Oxygen Traps?
Traces of oxygen in your carrier gas system can destroy a GC column, especially polar phases.
How does it work?
The Agilent OT3 Trap contains a highly active, metal-containing, scrubbing material in an inert, aluminum body. This material removes 500 mL oxygen from a gas stream to less than 15 ppb. Additionally, up to 2 grams of moisture can be removed by this trap without affecting the oxygen capacity.
Oxygen/Moisture Traps (OT3 Series)

Description Agilent OT3 Trap Agilent OT3 Trap Mounting Clip Size 100 cc 100 cc Fitting (in.) 1/8 1/4 Part No. OT3-2 OT3-4 MC-1
Agilent OT3 Trap
Hydrocarbon/Moisture Traps
Our hydrocarbon/moisture traps offer you flexibility: refill your trap and remove extremely low levels of both moisture and hydrocarbons. Replace most mixed bed traps supplied by GC manufacturers Mounting panel on page 70 Refillable
Hydrocarbon/Moisture Traps (HMT Series)

Description Refillable Hydro-Moisture Trap Refillable Hydro-Moisture Trap Mounting Clip for HMT Series Adsorbant Refill (1 pint) 2 Recharges for Hydrocarbon/Moisture Trap Size 200 cc 200 cc Fitting (in.) 1/8 1/4 Part No. HMT200-2 HMT200-4 MC-1 HCRMS
Hydrocarbon Moisture Trap
Combination Traps for Chemical Ionization MS and LC/MS

Gas Type Chemical Ionization for MS* LC/MS Nitrogen Gas Purifier** Fitting (in.) 1/8 1/4 Part No. G1999-80410 G1946-80047
*Isobutane or methane applications only. **Can be used up to 15 L/min. for LC/MS applications.
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Gas Management
Combination Traps Big Universal TrapsSuperior Gas Purifiers
Big Universal Traps utilize a layered, multi-adsorbent bed packing of the most effective, highest capacity adsorbent materials available today for the removal of oxygen, moisture, hydrocarbons, carbon dioxide and carbon monoxide from helium gas streams. The volume of the various adsorbent materials in the Big Universal Trap was developed through rigorous testing and evaluation in order to assure that breakthrough of the five major contaminant groups occurs as simultaneously as possible as each material achieves complete saturation. One Big Universal Trap unit will easily purify the contents of thirteen K size cylinders of 99.997% purity helium to a cumulative level of 100 parts per billion of O2, H2O, CO2, CO and hydrocarbons at a flow rate of up to 8 liters/minute. All tube fittings are Swagelok brand stainless steel, fitted with 40 micron stainless steel frits for particulate control. Maximum pressure is 500 psi.
Big Universal Traps are more economical than other purifiers. They cost only slightly more than competitive products, but offer nearly three times the capacity. Big Universal Traps are ideal for any GC or GC/MS application where helium is employed and assurance of purity is essential. Combining the contents of three individual in-line traps into a single unit reduces the number of potential leaks and the possibility of aspirating contaminants into the gas stream. A single purifier also simplifies installation and replacement.
Capacity Data
High Purity Helium99.997% Removal Capacity
Effluent Concentration
Research Grade Helium99.9999%
Impurities N2 O2 THC* CO+CO2 H2 O H2 Ar Ne
< 1.0 ppm < 0.5 ppm < 0.5 ppm < 0.1 ppm < 0.1 ppm < 0.2 ppm < 0.2 ppm < 0.1 ppm < 0.5 ppm
*Total Hydrocarbons, analysis limited to three contaminate groups
O2 THC* H2 O
< 5 ppm < 1 ppm < 5 ppm
1.07 L 20g 46g
*Total Hydrocarbons, analysis limited to three contaminate groups
Big Universal Traps have nearly three times the capacity of the competitors purifier
Big Universal Traps (RMS Series)

Gas Type Hydrogen Hydrogen
Big Universal Trap
Fitting (in.) 1/8 1/4 1/8 1/4 1/8 1/4
Part No. RMSHY-2 RMSHY-4 RMSH-2 RMSH-4 RMSN-2 RMSN-4 UMC-5-2

+Recommended for GC Mass Spec
Helium Helium
(Ar/Me)+ (Ar/Me)+
Nitrogen Nitrogen Big Mounting Clip, 2/pk
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Gas Management
Gas Purification Systems

Three Cartridge System
Agilents highest capacity and most economical gas purification system. Provides low-cost gas contaminant removal for up to 18 cylinders of carrier gas. Includes a manifold with cartridge mounts and three replaceable cartridges: one moisture/hydrocarbon cartridge; one oxygen cartridge; and one oxygen indicating cartridge. Designed to minimize column damage from impurities such as oxygen and to maximize performance by reducing sulfur compounds, moisture and organics to very low levels. First cartridge removes moisture and organics. Second cartridge provides the capacity to remove at least 1000 cc of pure oxygen. Third cartridge (indicating oxygen) changes color to warn of system saturation. Consists of a permanent all-welded stainless steel manifold to minimize the potential for leaks. Suitable for bench or wall mounting.
Single-Cartridge System
Three Cartridge High Capacity Gas Purification System
Contains a triple-combination cartridge that offers the same highly efficient contaminant removal properties from eight cylinders of carrier gas but without the visual indicator. Other cartridges are also available for detector gas supplies and as individual filters for specialized requirements.
Specifications: Three Cartridge High Capacity GPS

Max. Pressure: 200 psi Capacity: 1000 mL of oxygen, 18 tanks of 99.997% purity gas 15 g H20 (inlet at saturation) 0.7 g H20 (inlet moisture 10 ppm) 12 g hydrocarbon (as n-butane) Dimensions: Weight: Purity Level: 25 cm x 30 cm x 8 cm 4.8 kg 0.2 ppm oxygen; 99.8% removal of moisture and hydrocarbons
Specifications: Single Cartridge GPS

Max. Pressure: 200 psi Capacity: 450 mL of oxygen, 8 tanks of 99.997% purity gas 6.0 g H20 (inlet at saturation) 0.3 g H20 (inlet moisture 10 ppm) 5.2g hydrocarbon (as n-butane) Dimensions: Weight: Purity Level: 12 cm x 30 cm x 8 cm 1.6 kg 0.2 ppm oxygen; 99.8% removal of moisture and hydrocarbons
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Gas Management
Description Three Cartridge SystemIncludes manifold for wall or bench mount and 3 cartridges (H2O/hydrocarbon, oxygen, and indicating O2) With 1/8 in. fittings With 1/4 in. fittings Replacement Cartridge KitIncludes all three cartridges for above system. Single Cartridge SystemIncludes single cartridge manifold brackets for wall or bench mount, and a triple combination cartridge (H2O/hydrocarbon, and O2 ) 5183-1907 5182-9776 5182-9780 Part No.
With 1/8 in. fittings

Single Cartridge Gas Purification System
5183-4598 5183-4599 5182-9793 5182-9795
With 1/4 in. fittings Detector-Specific PurifiersFID and ECD FID Purifier (includes two hydrocarbon/moisture cartridges with separate connections for air and hydrogen lines) 1/8 in. fittings ECD Purifier (includes moisture cartridges and high capacity oxygen cartridge in series) 1/8 in. fittings Individual Cartridge Filters and Replacement Cartridges Moisture/hydrocarbon Oxygen Indicating oxygen Moisture only Hydrocarbon only Triple combination replacement cartridge for single cartridge system Single cartridge manifold, 1/8 in. fittings, to use with any of above cartridges for wall or bench mount Single cartridge manifold, 1/4 in. fittings, to use with any of above cartridges for wall or bench mount
5182-9777 5182-9778 5182-9779 5182-9792 5182-9791 5183-4600 5183-4607 5183-4608
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Gas Management
Gas Purification Systems QC+ Point of Operation Panel
The QC+ Point of Operation Panel contains purifier cartridges that can be quickly changed (thats the QC). The cartridges are removed from the panel without interruption of gas flow to the system (thats the +), drastically minimizing costly instrument downtime (and thats a real +). Filter cartridges are of all metal or glass construction, eliminating infusion and resultant signal noise associated with filters constructed from plastics. Cartridges are quickly installed via a simple knurled retaining nut. No wrenches are needed! As many as four cartridges can be replaced in a matter of seconds, and because there is low dead volume, a minimal amount of gas system purge is required after installation. Each QC+ filter head is equipped with an internal flow valve. This valve serves two functions. In the down position, gas is passed through adsorbent filter cartridges for purification (Figure 1b). In the up position, the gas is diverted through the filter head. With the valve up (Figure 1a), the filter cartridge can then be easily replaced with a fresh cartridge. But, because the gas continues to flow through the bypass valve, the need to shut down instruments is eliminated while the filter cartridges are removed. QC+ is the only GC purification system with this revolutionary flow through/bypass feature. The QC+ system has a unique D-shaped cartridge top that is self-aligning into the matching slotted filter head, assuring trouble-free installations. As many as four cartridges can be replaced in a matter of seconds without exposing downstream plumbing to atmospheric contaminants. QC+ systems are available in single-head kits or 2-, 3- and 4-head panel configurations, available exclusively with 1/8 in. Swagelok fittings. QC+ panels can be wall mounted or used freestanding. Oxygen, water, hydrocarbons and other organics are reduced to low part per billion levels.
1a
Replacement Cartridges
High Capacity Oxygen
The GC-1 oxygen cartridge is an all metal assembly that contains a highly active metal catalyst, supported on an alumina silicate substrate. Oxygen removal efficiency to less than 15 ppb. P/N: GC-1; Capacity: 396 mg
High Capacity Moisture
1b Figures 1a and 1b. The plus valve (a) features a closed gas path when changing cartridges. When placed in the down position (b), the plus valve shifts gas through the cartridge.
The GC-2 moisture cartridge is an all metal assembly that contains 13X molecular sieve, 1620 mesh sphere size. Water removal efficiency to less than 9 ppb. P/N: GC-2; Capacity: 15 g
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Gas Management
Replacement Cartridges (continued)
Indicating Moisture
The GC-2-I indicating moisture cartridge is a glass assembly that contains a combination of 5 molecular sieve and indicating Drierite. This cartridge provides a visual indicator of adsorbent saturation. Water removal efficiency to less than 9 ppb. P/N: GC-2-I; Capacity: 7 g
Hydrocarbon
The GC-3 hydrocarbon cartridge is an all metal assembly that contains a high surface area and coconut shell-based, activated carbon medium. Efficiencies to low ppb levels for C2 and heavier compounds. Hydrocarbon removal efficiency to less than 30 ppb. P/N: GC-3; Capacity: 8 g
Indicating Oxygen
The GC-4 indicating oxygen cartridge is constructed from glass. It contains a manganese oxide adsorbent that experiences a dramatic and progressive color change during adsorption. Oxygen removal efficiency to less than 2 ppb. P/N: GC-4; Capacity: 40 mg
2-, 3- and 4-Head Panels

2-Head GC Carrier Gas
QC+ System RBC-P Two-head carrier gas purification system is strongly recommended when employing columns with bonded phases or any capillary column. Consists of high capacity oxygen/moisture adsorber and indicating trap that signals breakthrough of high capacity, non-indicating cartridge. Flow at Pressure: 13 LPM at 50 psi helium
2-Head Detector Gas Supply for FID
QC+ System RBC-D-P Two-head air/hydrogen system is strongly recommended for both hydrogen and air supply to Flame Ionization Detectors (FIDs). Includes high capacity moisture and hydrocarbon cartridges. Flow at Pressure: 14 LPM at 50 psi helium
3-Head GCs with Selective Detectors and Carrier Gas
QC+ System RTC-P Three-head cartridge system with high capacity oxygen, moisture, and hydrocarbon cartridges. The perfect compliment to any system that employs nitrogen, helium, or argon/methane mixtures. Flow at Pressure: 7 LPM at 50 psi helium
3-Head Electron Capture and ELCD Detectors
QC+ System RTC-I-P Three-head cartridge panel that includes high capacity oxygen, moisture, and indicating oxygen cartridges. Flow at Pressure: 7 LPM at 50 psi helium
4-Head GC/MS and any Critical GC Application
QC+ System RQC-P Consists of high capacity oxygen, moisture, and hydrocarbon removing filters, plus an indicating oxygen cartridge to signal oxygen breakthrough of upstream unit. The ultimate in gas purification for any GC application. Flow at Pressure: 6 LPM at 50 psi helium
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Gas Management
Quick Change Plus (QC+) Point of Operation Panels
Description
1-Head
Part No. R2D2-P R2D2-I-P
High capacity oxygen Indicating oxygen

2-Head
High capacity oxygen, ind. oxygen Moisture, hydrocarbon Indicating moisture and hydrocarbon Moisture and indicating moisture
3-Head
1/8 1/8 1/8 1/8
RBC-P RBC-D-P RBCD-P-2I/3 RBCDP-GC2/I
RBC-P
Oxygen, moisture, hydrocarbon Moisture, oxygen, ind. oxygen

4-Head
1/8 1/8
RTC-P RTC-I-P
O2, indicating O2, HC, H2O
1/8
RQC-P
RTC-I-P
Replacement Cartridges
Description High capacity oxygen High capacity moisture Indicating moisture Hydrocarbon Indicating oxygen Replacement O-ring set Part No. GC-1 GC-2 GC-2-I GC-3 GC-4 R2D2-ORINGS
RQC-P
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Gas Management
Gas Purification Systems Super-Clean Gas Filter System
Super-Clean gas filter systems are designed to provide the utmost in convenience and contamination reduction. The system is tested for leak-tightness and the glass and metal construction of the cartridges eliminates diffusion of contaminants into the gas stream. During cartridge replacement, check valves close off the system to the atmosphere, further minimizing the entry of contaminants. Filter systems are available in several configurations, including 3 and 4 cartridge systems for GCs equipped with Flame Ionization Detectors (FIDs). Additionally, baseplates are available for 2, 3, or 4 filters, allowing the user to design a gas filter system for any application. Unit is ready to connect with 1/8 in. tubing. Maximum pressure is 150 psi (11 bar). Triple filter cartridge for carrier gas purification with a single cartridge. Fuel gas filter for removal of hydrocarbons and moisture from FID fuel gases. The filter's gas flow path is made from high quality glass and stainless steel. Filters can be changed easily, without tools. Base plate design allows customization for your gas filtration needs (see figure). Indicating action tells when to replace cartridges.
Gas Filter Base Plate Design
Helpful Hint: Built-in check valves automatically stop gas flow to allow for fast filter changes without the introduction of ambient air into the system.
Specifications Max pressure: Capacity: 150 psi (11 bar) Triple Filter1.8 g H2O, 0.5 L O2 Fuel gas filter3.6 g H2O Moisture filter7.2 g H2O Oxygen1000 mL of O2 Dimensions: Weight: Purity level: 33 cm x 31 cm x 11 cm4 filter system 8 kg 0.1 ppm at max flow rate of 2 L/min (max pressure 10 bar)
Super-Clean Gas Purification Systems

Description Part No. FID Gas Purification System: Dedicated three position system for purifying 5182-9703 all gases used in an FID-equipped GC. Includes the Triple Filter Cartridge for carrier gas, and two fuel gas filter cartridges for both hydrogen and zero air. Carrier Gas Purification System: Single position system perfect for GC/MS, ECD and NPD detectors. Includes the triple filter cartridge. Super-Clean Gas Purification System: For your most demanding GC application, includes four position baseplate manifold with four filter cartridges: oxygen, moisture (both with indicator), and two hydrocarbon cartridges. 5182-9704 5182-0816
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Gas Management
Super-Clean Gas Purification Systems (continued)
Description FID Fuel Gas Purification System: Two position system dedicated to purifying the incoming hydrogen and zero grade air directly at your FIDequipped GC. Replacement Filter Cartridges Triple Filter Cartridge: A single carrier gas filter now with hydrocarbon, moisture, and oxygen trapping capability. Includes moisture and oxygen indicator so you know exactly when to replace the cartridge. Fuel Gas Filter Cartridge: Combination hydrocarbon and moisture trap with moisture indicator. Insures the highest quality hydrogen and zero air for your FID. Moisture filter cartridge with indicator Oxygen filter cartridge with indicator Hydrocarbon filter cartridge Filter cartridge bundle of 4 (oxygen, moisture, and 2 hydrocarbon) FID Filter Cartridge Bundle - includes one Triple Filter Cartridge and two Fuel Gas Filter Cartridges. Design your own gas purification system - use the following baseplates and available filter cartridges to design a customized gas solution specific to your application needs! Single position baseplate/manifold 2 position baseplate/manifold 3 position baseplate/manifold 4 position baseplate/manifold Wall mount bracket for Super-Clean GPS Super-Clean GPS O-ring kit (8/pk, 4 ea. of 2 sizes) 5182-0815 5182-9706 5182-9707 5182-9713 5182-0821 5182-3423 5182-9705 Part No. 5183-4774
5183-4771
5182-0817 5182-0818 5182-0820 5183-4770 5183-4769
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Gas Management
Gas Purifier Accessories Universal/External Split Vent Trap
Split vent trap stops environmental pollution. The split vent trap was designed to protect the lab environment from the contaminants released by split injection systems, which can vent up to 500 times the amount of sample reaching the detector into the laboratory's air. A replaceable, impregnated carbon filter media traps and eliminates a broad range of contaminants. The traps are also easy to change and come with three packs of replacement cartridges each. Replace approximately every six months.
Split Vent Trap and Cartridges P/N RDT-1020
Description Universal/external split vent trap with 3 cartridges (1/8 in. Swagelok fitting) Replacement cartridges (3/pk)
Part No. RDT-1020 RDT-1023
Chemical Filters/Traps for GC 5890

These filters are packed with molecular sieve 5, 60/80 mesh, and can be reconditioned by heating in the GC oven. (Recommended replacement for P/N 5060-8299)
Description Chemical filter for 5890 internal Detector trap replacement kit Replacement detector stainless steel trap Replacement O-rings Plastic fitting Split vent trap for 5890 GC (internal) 12/pk 10/pk Units Part No. 05890-61260 19231-60790 19231-80590 5180-4181 5181-3394 19251C
Trap Mounting Panel and Mounting Clips

17 in. x 24 in. Five rows for mounting traps Designed for MC-1, UMC-2, UMC-3, UMC-4, and UMC-5 mounting clips
Trap Mounting Panel

Description Trap Mounting Panel Part No. UMP-1724
Mounting Clips for Traps

Description Clip, 120-200 cc, 7/8 1 in. od Use with Trap Series MT120, MT200, OT1, OT3, MT3, HT200, HMT Part No. MC-1 UMC-2 UMC-3 UMC-4 UMC-5 UMC-5-2
*Big Traps require two mounting clips.
Clip, universal 1-1/8 1-3/8 in. od IOT

Mounting Clip P/N MC-1
Clip, universal 1-3/8 1-5/8 in. od GMT, LIOT Clip, universal 1-5/8 1-7/8 in. od MT400, LGMT Clip, universal 2 2-1/2 in. od, 1 each Clip, universal 2 2-1/2 in. od, (2/pk) Big Traps* Big Traps*
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Gas Management
GC Installation Kits
Agilent GC Installation Kits

Agilent Installation Kits for GC are designed to provide you with the essential items necessary for a successful installation while providing a substantial cost savings over individual purchases.
Description Installation Kit with Tools for Agilent 6890 GCs without Gas Purifiers, includes: Snoop leak detector Nuts and ferrules kit, 1/8 in., brass Copper tubing, 50 ft x 1/8 in. 1/8 in. Tees, brass Tubing cutter, 1/8 in. to 5/8 in. od tubing 1/8 in. brass caps Nut driver, 7 mm Screwdriver, Torx T 10 Screwdriver, Torx, T 20 Wrench, column nut, 1/4 in. x 5/16 in. Wrench, open end, 7/16 in. x 9/16 in. Wrench, open end, 7/16 in. and 3/8 in. Wrench, open end, 7/16 in. and 1/2 in. Basic Installation Kit for Agilent 6890 GCs with Carrier Gas Purifier Train (no tools), includes: Snoop Nuts and ferrules kit, 1/8 in., brass Copper tubing, 50 ft. x 1/8 in. 1/8 in. Tees, brass Tubing cutter, 1/8 in. to 5/8 in. od tubing 1/8 in. brass caps Oxygen trap, 1/8 in. glass indicating Big Oxygen Trap, 1/8 in. high capacity, 750 cc Moisture trap, 100 cc, 1/8 in. glass indicating Activated charcoal trap, S-type, 1/8 in. Split vent trap kit 6/pk 2/pk (2 packs of 2) 20/pk 6/pk 2/pk (2 packs of 2) 20/pk Unit Part No. 19199M 9300-0311 5080-8750 5180-4196 5180-4160
8710-1709 5180-4121 8710-1217 5182-3466 5182-3465 8710-0510 8710-0803 8710-0972 8710-0806 19199N 9300-0311 5080-8750 5180-4196 5180-4160 8710-1709 5180-4121 IOT-2-HP BOT-2 GMT-2-HP 5060-9096 RDT-1020
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Gas Management
GC Installation Kits
Description Basic Installation Kit for Agilent 4890 GCs, includes: Nuts and ferrules kit, 1/8 in. brass Cap, 1/8 in. brass Tee, 1/8 in. brass Union
Basic installation kit for 4890 GC
Unit 20/pk 6/pk 2/pk (2 packs of 2)
Part No. 19199P 5080-8750 5180-4121 5180-4160 5180-4196 8710-1709 9300-0311 0101-0113
Copper tubing, 50 ft. x 1/8 in. Tubing cutter, 1/8 in. to 5/8 in. od tubing Snoop leak detector 8 oz. Glass Bubble Flowmeter 100 mL Syringe 10 L, FN, 23 ga. 6/pk Open end wrench, 7/16 in. and 3/8 in. Nut, driver Screwdriver, 4 in. Pozidriv shaft No. 2 pt Screwdriver, 3 in. Pozidriv shaft No. 1 pt Wrench, column nut, 1/4 in. x 5/16 in. Wrench, open end, 7/16 in. and 9/16 in. Wrench, open end, 1/2 in. and 7/16 in.
9301-0511 8710-0972 8710-1217 8710-0900 8710-0899 8710-0510 8710-0803 8710-0806
Miscellaneous Items Teflon tape Copper tubing, 50 ft. x 1/8 in. Tubing cutter, 1/8 in. to 5/8 in. od tubing General purpose fittings kit GC basic installation kit, includes copper tubing, column nuts, two moisture traps, and five wrenches 1 roll 0460-1266 5180-4196 8710-1709 5180-4161 5182-3453
Fittings Kit P/N 5780-4161
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Gas Management
Flowmeters
Flowmeters
Agilent has a flowmeter for every application...
Agilent manufactures the largest selection of flowmeters for chromatography. We have developed flowmeters for measuring capillary column flows, calibrating air pumps and flow controllers, and verifying instrument gas flows. Setting and maintaining GC flow rates greatly affect the instrument accuracy and sensitivity. Choosing a flowmeter for your application depends upon measurement speed, ease of use, accuracy and flow rate range.
Measurement Speed
Agilent offers two types of volumetric flowmetersbubble (Optiflow) and acoustic displacement (ADM and FlowTracker series)and one mass flowmeter (Veri-Flow 500). Volumetric measurements rely upon the volume of gas passing through the flow meter but not the composition. Additionally, volumetric flowmeters accurately measure from a mixed or multicomponent gas stream. Mass flowmeters are calibrated to specific gas compositions and determine the flow rate for those calibrated gases. Mass measurements are independent of temperature and pressure. With volumetric flowmeters, no adjustments are needed when switching from one gas stream to a different composition stream. So an analyst can immediately change from measuring nitrogen from a GC detector to helium split rate from an injection port, to methane from an anaerobic digestion vessel. If a mass flowmeter is used, the analyst must change instrument settings and purge the flowmeter of the initial gas before accurate readings can be taken.
Ease of Use
When setting gas flows with many flowmeters, the analyst often needs more than two hands to hold and start the flowmeter. With continuous flow readings, the analyst does not have to start or stop the flowmeter with each measurement. The FlowTracker, Veri-Flow 500, and ADM flowmeters read flow values continuously.
Flow Rate Range
Low flow rates? No problem (down to 0.1 mL/min). Very high flow rates? No problem (up to 5 L/min). A wide range of flow rates? No problem (0.5-1000 mL/min using a single flowmeter).
Accuracy
Agilent accurately calibrates all flowmeters to NIST-traceable standards.
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Gas Management
Flowmeters
Flowmeter Selection Guide
Digital Flowmeters: For those who prefer data acquisition and logging with completely electronic, multifunction devices. For noncorrosive gas streams.
Description Low Flow (mL/min) High Flow (mL/min) Flow through design Gases measured (non-corrosive) Volumetric1 or mass2 flowmeter RS232 capability Unique features Power supply Page No.
Accuracy
FlowTracker 2000 03 plus Leak Detector
500
2%
Yes
N2, H2, He, Air, CO2, CH4, 95% Ar/CH4 N2, H2, He, Air, CO2, CH4, 95% Ar/CH4 All
Both
RS232 output Multifunction digital volumetric flowmeter, with leak detection and pressure, temperature, and calculated mass flow measurements RS232 output Multifunction digital volumetric flowmeter, with pressure, temperature, and calculated mass flow measurements RS232 output Easy-to-use digital volumetric flowmeter, with calculated mass flow measurement and RS232 output none Economical digital volumetric flowmeter for most chromatographic flow measurements True mass flow measurement with RS232 input and output capability
6 AA batteries or 110v/220v AC
75
FlowTracker 1000
03
500
2%
Yes
Both
6 AA batteries or 110v/220v AC 9v battery or 110v/220v AC 9v battery
75
ADM 2000
0.5
1000
3%
No
Both
7677
ADM 1000
0.5
1000
3%
No
All
Volumetric
7677
Veri-Flow 500
500
3%
Yes
N2, H2, He, Mass Air, 95% Ar/CH4
RS232 input and output
Rechargeable battery or 110v/220v AC
77
Bubble Flowmeters: For those who prefer classic bubblemeter technology combined with digital readout. Durable, for all gases, including mildly corrosive and humid gas streams.
Description Low Flow (mL/min) High Flow (mL/min) Flow through design Gases measured Volumetric1 or mass2 flowmeter RS232 capability Unique features Power supply Page No.
Accuracy
Optiflow Model 420 Optiflow Model 570 Optiflow Model 650
0.1 0.5
50 700
3% 3%
No No
All All
Volumetric Volumetric
none none
Low flow range for measuring flows of capillary columns Medium flow range for general chromatography applications, also available in a field portable case High flow range for demanding flow measurements, including negative (vacuum) flows, also available in a field portable case
9v battery 9v battery
7879 7879
5000
2%
No
All
Volumetric
none
9v battery
7879
Liquid Flowmeters: For those who need to verify and set HPLC column flow rates
Description Low Flow (mL/min) High Flow (mL/min) Flow through design Liquids measured Volumetric1 or mass2 flowmeter RS232 capability Unique features Power supply Page No.
Accuracy
Optiflow Model 1000
30
2%
No
HPLC mobile phases
Volumetric
none
Unique flowmeter for measuring flows of common HPLC mobile phases
110v/220v AC
7879
1 Volumetric measurements are accurate and are independent of the gas that is being measured, thus allowing measurements of mixed gases commonly found in chromatography. 2 Mass flow measurements are very accurate for specific gases and are not affected by temperature and pressure fluctuations. 3 FlowTrackers measure flow rates very accurately down to 5 mL/min; from 0 - 5 mL/min, the standard deviation increases to approximately 5%
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Gas Management
Flowmeters FlowTrackers
Agilents FlowTracker Flowmeters are the ultimate gas flowmeters for chromatography applications. These handheld flowmeters incorporate industry leading performance and features in a highly accurate and reliable package. The inherent stability of the rugged, solid state components allows us to provide the longest calibration interval on the market, all traceable to NIST standards. There are two models available:
FlowTracker 1000
Highly accurate and reliable measurement of common carrier and fuel gases used in GC including: nitrogen, air, oxygen, hydrogen, helium, and argon/methane Two year guaranteed calibration period traceable to NIST standards Measures flow (0-500 mL/min.) based on gas viscosity properties with an accuracy of +/-2%, (+/-5% from 0-5 mL/min.) Flow rate range from 0-500 mL/min (no conversion necessary from SCCM) eliminates the need for two separate meters to measure capillary and standard flows Displays mass flow, volumetric flow, temperature, and pressure readings simultaneously Screen displays for flow, linear velocity, and split ratio modes
FlowTracker 1000 P/N 5183-4779
FlowTracker 2000
Combination Flowmeter/Leak Detector: the ultimate GC troubleshooting tool! All the features of the FlowTracker 1000 plus an economical Thermal Conductivity Leak Detector in a single handheld design (leak detection at 10-3 cc/s vs. 10-5 cc/s for most dedicated leak detectors, see page 80) First of its kind product to hit the market Industry-leading flowmeter performance with leak detection for less than the price of a typical leak detector Visual and audible detection signals Cost effective alternative to purchasing two separate instruments to measure GC flows and detect gross leaks in your GC and GC/MS systems For complete specifications, ordering information, and availability, please contact your local Agilent sales office, an authorized Agilent dealer, or visit our web site.
FlowTracker 2000 P/N 5183-4780
FlowTracker Flowmeters
Description FlowTracker 1000 Flowmeter FlowTracker 2000 Flowmeter and Leak Detector FlowTracker Accessory Kit, includes adapters for various tubing sizes FlowTracker Universal AC Adapter (optional, not supplied with FlowTracker units) Part No. 5183-4779 5183-4780 5183-4788 5183-4781
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Gas Management
Flowmeters The ADM Flowmeter
Want to use the simplest flowmeter for gas chromatographic analysis? ADM flowmeters allow most gas measurements with the touch of only one buttonturn it on, and the instrument provides continuous, hands-free flow readings. You can measure column, detector, and carrier gas flows without any adjustments. ADM flowmeters are ideal for measuring gas streams with a changing gas composition. For example, if you measure the gas flow from a digestion system, concentration changes in methane, carbon dioxide, and oxygen will not affect accuracy. All ADM flowmeters are battery powered and field portable. Agilent calibrates each instrument to 5-point, NIST-traceable standards to ensure the highest available accuracy. The ADM family measures flow volumetrically, so you dont have to make any adjustments when changing from one gas to another.
If you need rapid, real-time measurements of a gas stream, ADM Flowmeters are perfect for you. These flowmeters are designed for basic gas chromatography laboratories.
ADM 1000 features include:

Accuracy 3% Operating temperature range0 to 45C for the instrument, -70 to 135C for the tubing. Calibrationtraceable to NIST primary standards Real time, split ratio measurement CE mark certified Measures flow rates from 0.5- to 1000 mL/min Split ratioscompare the ratio from one gas measurements to another (i.e., injection port split ratios)
ADM 1000
ADM 2000
In addition to the features of the ADM 1000, the ADM 2000 includes: Mass flow measurementsmeasure flow rate, independent of atmospheric pressure and temperature (calculated) Data output through RS-232 port 9V battery and AC power adapter (120 or 220 VAC)
ADM 2000
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Gas Management
Flowmeters
ADM Flowmeters
Description Flow Rate (mL/min) Low High Gases* Measured Accuracy (%) Power Supply RS-232 Data Output Part No.
ADM1000 ADM2000 ADM2000E Carrying Case for ADM
0.5 0.5 0.5
1000 1000 1000
All All All
3 3 3
9V Battery Battery or 120 VAC Battery or 220 VAC
None Yes Yes
220-1170 220-1171-U 220-1171-E 907-0056

*Non-corrosive Gases Only
Veri-Flow 500
Need the ultimate in versatility? The Veri-Flow 500 packs an incredible amount of features into a highly accurate flowmeter. The solid state gas sensor used in the Veri-Flow 500 measures mass flow rates of the 5 common GC laboratory gases from 5 to 500 mL/min. Above all, the Veri-Flow 500 has big features for a small, small price.
What Does it Measure?
5 gasesH2, He, N2, Air, Argon/Methane In-line flow Injection port split flows Carrier gas linear velocity
What Else?
Two-way data communication through serial port Rechargeable battery pack 3% accuracy Calibrated to a NIST primary standard CE mark certified
Electronic Mass Flowmeters

Description Flow Rate (mL/min) Low High Gases* Measured Accuracy (%) Power Supply RS-232 Data Output Part No.
Veri-Flow 500 (110 V) Veri-Flow 500 (220 V)
5.0 5.0
500 500
He, H2, Ar/CH4 N2, Air He, H2, Ar/CH4 N2, Air
3 3
Rechargeable Battery or 110 VAC Rechargeable Battery or 220 VAC
Yes Yes
HVF-500 HVF-500-2
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Gas Management
Flowmeters Digital Bubble Flowmeters
Agilent Digital Bubble Flowmeters have been making flow measurement easy for years. Today, they are still widely used to measure GC flows, calibrate air sampling pumps, and verify various gas flows. The Optiflow series offers the widest range of flow rates and allows you to visually ensure gas flows. This tried and true flowmeter measures volumetrically and therefore can measure any gas composition with the push of one button and the squeeze of a bulb.
Optiflow Features:
Calibrated to NIST primary standards Wide flow range from 0.1 to 50 mL/min (Optiflow 420) up to 5 to 5000 mL/min (OptiFlow 650) 9V Long-lifetime battery Available with hard field case (Models 570 and 650 only)
Optiflow 420
The Optiflow 420 is the best bubble flowmeter for measuring low gas flow ratesespecially GC columns. Flow range0.1 to 50 mL/min +/- 3% accuracy
Optiflow 420
Optiflow 570
Want a bubble flowmeter for measuring carrier, makeup, injection port, and detector gases? The Optiflow 570 handles most of your GC needs. We also manufacture Optiflow 570 in a hard metal case, which makes it portable and field ready. Flow range0.5 to 700 mL/min for the Optiflow 570 Available in hard field case (Optiflow 570 only) Split ratio measurement +/- 3% accuracy
Optiflow 570
Optiflow 650
If you calibrate air sampling equipment, verify mass flow controller settings, or monitor higher gas flow rates, these Optiflow flowmeters are simple and efficient. Flow range5.0 to 5000 mL/min for the Optiflow 650 +/- 2% accuracy Available in hard field case Measures both positive and negative (vacuum) flow sources
Optiflow 650
Optiflow 1000Liquid Flowmeter

Optiflow 1000 measures liquids! Verify and set HPLC column flow rates with the Optiflow 1000. Flow range1 to 30 mL/min +/- 2% accuracy
Optiflow 1000
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Gas Management
Flowmeters
Optiflow Flowmeters
Gas Flowmeters Description Field Case Flow Rate (mL/min) Low High Gases* Measured Accuracy (%) Power Supply RS-232 Data Output Part No.
Optiflow 420 Optiflow 570 Field-Cal 570 Optiflow 650 Field-Cal 650
No No Yes No Yes
0.1 0.5 0.5 5.0 5.0
50 700 700 5,000 5,000
All All All All All
+/- 3 +/- 3 +/- 3 +/- 2 +/- 2
9V Battery 9V Battery 9V Battery 9V Battery 9V Battery
None None None None None
HFM-420 HFM-570 HFM-570-FC HFM-650 HFM-650-FC
Liquid Flowmeters
Optiflow 1000 (110V) Optiflow 1000 (220V)
1 1
30 30
+/- 2 +/- 2
AC Adapter, 110V AC Adapter, 220V
None None
HFM-1000
HFM-1000-2
*Noncorrosive and Mildly Corrosive Gases Only
Economy Glass Bubble Flowmeters

Description 10 mL Glass Bubble Flowmeter 100 mL Glass Bubble Flowmeter Part No. 0101-0030 0101-0113
Accessories for Bubblemeters

Description Replacement Bulbs for Optiflow and Glass Bubble Flowmeters Snoop, 8 oz. Part No. HFM-BULB 9300-0311
Glass Bubble Flowmeters
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Gas Management
Leak Detectors
Leak Detection
Gas Leak Detector
The Agilent Technologies Gas Leak Detector enables quick and easy detection and measurement of gas leaks. Based on a dual cell micro volume thermal conductivity system, this unit provides very high sensitivity and eliminates contamination caused by soap solution methods. This hand-held, battery-powered detector indicates leak magnitude on the display and provides a proportional audible signal. Detachable probe and flex-and-stay extension allows access to the most difficult to reach areas. Built-in calibration for 12 gases. AC adapter/battery charger included. Helpful Hint: Using a leak detector avoids system contamination from soap solution detection methods.
Specifications
Response time:
Gas Leak Detector
Approximately one second Hydrogen, Helium, Neon, Xenon, R11, R12, R21, R22, Methane, Argon, CO2, Water Vapor He 1x10-5, R12 8x10-5, CH4 1x10-4, CO2 1x10-4 (mL/sec) Dual cell micro volume thermal conductivity 6.75 in. (l) x 3 in. (w) x 1 in. (d) (172 mm x 77 mm x 26 mm) 8 in. (l) x 1.85 in. (d) (204 mm x 28 mm) Rechargeable NiCad battery, charger/AC adapter
Built-in calibration: Max. sensitivity under ideal conditions: Detection principle Console size: Probe size: Power:
Gas Leak Detector Kit

Description Leak detector includes probe, extended flexible probe, range extension nozzle, probe clip and template, cable, AC power adapter/battery charger, battery, user manual, cleaning wipe, and carrying case (available in 115 V or 220 V). Gas leak detector, 115 V Gas leak detector, 220 V Part No.
5182-9646 5182-9648
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Gas Management
Regulators and Accessories
Regulators & Tubing

Gas Cylinder Regulators
To plumb your system properly, select one of Agilents economical brass regulators and the proper gas purification devices from our newly expanded product offering. These regulators and our broad spectrum of in-line filters, combination traps, and gas purification systems are an excellent combination to plumb your systems. Our new gas purification selection guide and diagrams on pages 4849 will help you determine which products can be used with the regulators below. Helpful Hint: Always depressurize a regulator before closing the adjusting knob and removing the regulator from the cylinder.
Brass Body, Dual Stainless Steel Diaphragms

Specifications
Max inlet pressure: 3000 psig Operating -40F to 165F Temperature range: Inlet-outlet port: Outlet Valve: 1/4 in., MNPT 1/4 in., MNPT 2 1/2 in. Dual scale gauges CGA-346, 350, 340, 580, 590 Internal self-reseating relief valve 5.6 lbs
Materials of Construction
Body: Bonnet: Diaphragms: Seat material: Seat assemblies: Gauges: Adjusting knob: Brass Brass Stainless steel P.T.F.E. Encapsulated Brass A.B.S. plastic
Brass Body Regulator
Outlet needle valve: Brass
Delivery pressures: 1 to 125 psig Weight:
Brass Body, Dual Stainless Steel Diaphragms (1/8 in.)*

Description CGA 346, 125 psig max (8.6 bar), Air CGA 350, 125 psig max (8.6 bar), H2, Ar/Me CGA 540, 125 psig max (8.6 bar), O2 CGA 580, 125 psig max (8.6 bar), He, Ar, N2 CGA 590, 125 psig max (8.6 bar), Air Part No. 5183-4641 5183-4642 5183-4643 5183-4644 5183-4645
*For 1/4 in. tubing, purchase a 1/4 in. adapter listed below
Regulator Outlet AdaptersFemale NPT to Swagelok-style

Description 1/4 in. to 1/8 in. brass (included with brass regulators) 1/4 in. to 1/4 in. brass* Part No. 0100-0118 0100-0119
*Required for plumbing 1/4 in. tubing to regulators above
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Gas Management
Regulators and Accessories TubingPrecleaned
Description Copper tubing, 1/8 in., 50 ft. Copper tubing, 1/8 in., 12 ft. Part No. 5180-4196 5021-7107
Spectra-Link is No Ordinary Flexible Tubing!

Stainless Steel: no outgassing or permeation through polymeric materials Quick Connection: prevents air from entering gas lines during tank changeover Tested: each system has leak rates lower than 1x10-5 cc/sec
Description Part No. SL-8 SL-4
Spectra-Link with 1/8 in. fittings and 36 in. SS tubing Spectra-Link with 1/4 in. fittings and 36 in. SS tubing
Spectra-Link Tubing Connecting System on a two-stage regulator
Cylinder Bracket
Description Cylinder wall bracket with strap and chain (cylinder size up to 14 in., 35 cm) Part No. 5183-1941
Cylinder Wall Bracket P/N 5183-1941
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Hardware
Fittings
Nuts, Ferrules, Caps, and Plugs

Nuts, Front and Back Ferrule Sets
Description 1/16 in. SS 1/8 in. Brass 1/8 in. SS 1/4 in. Brass 1/4 in. SS Unit 10/pk 20/pk 20/pk 20/pk 20/pk Part No. 5180-4149 5080-8750 5080-8751 5080-8752 5080-8753
Nut Nut, Front and Back Ferrule Sets
Nuts
Description 1/16 in. nut, SS* 1/8 in. nut, Brass 1/4 in. nut, Brass Unit 10/pk 10/pk 10/pk Part No. 5180-4102 5180-4103 5180-4105
Front Ferrules
Description 1/16 in. front ferrule, SS 1/16 in. front ferrule, Valco one piece, SS 1/8 in. front ferrule, Brass 1/4 in. front ferrule, Brass Unit 10/pk 10/pk 10/pk 10/pk Part No. 5180-4108 5181-1292 5180-4109 5180-4111
Front Ferrules
Back Ferrules
Back Ferrules
Description 1/16 in. back ferrule, SS 1/8 in. back ferrule, Brass 1/4 in. back ferrule, Brass Unit 10/pk 10/pk 10/pk Part No. 5180-4114 5180-4115 5180-4117
Cap
Caps
Description 1/8 in. cap, Brass 1/4 in. cap, Brass Unit 6/pk 6/pk Part No. 5180-4121 5180-4120
Plug
Plugs
Description 1/8 in. plug, Brass 1/4 in. plug, Brass Unit 6/pk 6/pk Part No. 5180-4124 5180-4125
Blanking Nut, Adapter, and Hose Connector

Description 1/16 in. blanking nut, SS 1/8 in. x 1/8 in. adapter, Brass 1/16 in. hose connector, Brass Unit 1/ea 1/ea 10/pk Part No. 01080-83202 0100-0420 5180-4148
* SS = Stainless Steel
Blanking Nut
Adapters
Hose Connector
83
Hardware
Fittings
Tees, Crosses, and Elbows

Tees
Description
Tee
Unit 1/ea 2/pk 1/ea 2/pk
Part No. 0100-0782 5180-4160 0100-0542 5180-4129
1/16 in. tee, SS 1/8 in. tee, Brass 1/8 in. tee, SS 1/4 in. tee, Brass
Low Dead Volume Tee

Low Dead Volume Tee
Description 1/16 in. union tee, low dead volume, SS
Unit 1/ea
Part No. 0100-0969
Crosses
Description 1/8 in., Brass
Cross
Unit 1/ea 1/ea
Part No. 0100-0161 0100-0985
1/4 in., Brass
Elbows
Description 1/8 in., Brass Unit 1/ea Part No. 0100-0091
Elbow
Unions and Connectors

Unions
Description 1/16 in. union, SS 1/16 in. union, Brass Unit 1/ea 1/ea 2/pk 1/ea 2/pk 1/ea Part No. 0100-0124 0100-1316 5180-4127 0100-0126 5180-4128 0100-0128
Union
1/8 in. union, Brass 1/8 in. union, SS 1/4 in. union, Brass 1/4 in. union, SS
Zero Dead Volume Union

Zero Dead Volume Union
Description 1/16 in. union, zero dead volume, SS
Unit 1/ea
Part No. 0100-0900
Bulkhead Unions
Description 1/8 in. bulkhead union, Brass
Bulkhead Union
Unit 1/ea 1/ea
Part No. 0100-0132 0100-0133
1/8 in. bulkhead union, SS
84
Hardware
Fittings
Connectors (Swagelok-style to Male NPT)
Description 1/8 in. (A) 1/4 in. (B) connector, Brass 1/4 in. (A) 1/4 in. (B) connector, Brass Unit 2/pk 2/pk Part No. 5180-4143 5180-4145
Connector (Swagelok-style to Male NPT)
Connectors (Swagelok-style to Female NPT)

Description 1/8 in. (A) 1/4 in. (B) connector, Brass 1/4 in. (A) 1/4 in. (B) connector, Brass Unit 1/ea* 1/ea* Part No. 0100-0118 0100-0119
* Use with regulators on page 81.
Adapters and Reducers

Reducing Unions
Description 1/16 x 1/8 in. reducing union, Brass 1/16 x 1/8 in. reducing union, SS 1/8 x 1/4 in. reducing union, Brass 1/8 x 1/4 in. reducing union, SS Unit 2/pk 1/ea 2/pk 1/ea Part No. 5180-4130 0100-0241 5180-4131 0100-0121
Connector (Swagelok-style to Female NPT)
Reducing Union
Reducers
Description 1/8 in. (A) 1/4 in. (B) reducer, Brass 1/4 in. (A) 1/8 in. (B) reducer, Brass Unit 2/pk 2/pk Part No. 5180-4135 5180-4134
Reducer
Fittings Kit
Description Fittings Kit-includes items below Nut, 1/8 in., Brass Nut, 1/4 in., Brass Ferrules, front 1/8 in., Brass Ferrules, front 1/4 in., Brass Ferrules, back 1/8 in., Brass Ferrules, back 1/4 in., Brass Cap, 1/4 in., Brass Cap, 1/8 in., Brass Plug, 1/8 in., Brass Plug, 1/4 in., Brass Union, 1/8 in., Brass Union, 1/4 in., Brass Union, tee, 1/8 in. Brass Union, tee, 1/4 in., Brass Union, 1/4 x 1/8 in. reducing, Brass Reducer, 1/4 x 1/8 in., Brass Reducer, 1/8 x 1/4 in., Brass www.agilent.com/chem 10/pk 10/pk 10/pk 10/pk 10/pk 10/pk 6/pk 6/pk 6/pk 6/pk 2/pk 2/pk 2/pk 2/pk 2/pk 2/pk 2/pk Unit Part No. 5180-4161 5180-4103 5180-4105 5180-4109 5180-4111 5180-4115 5180-4117 5180-4120 5180-4121 5180-4124 5180-4125 5180-4127 5180-4128 5180-4160 5180-4129 5180-4131 5180-4134 5180-4135
Fittings Kit
85
Hardware
Tools
Tools
The General Laboratory Tool Kit includes the following items: Toolbox Flashlight 8 in. bastard file 6 in. adjustable wrench 12 in. adjustable wrench for use with regulator No. 1 pt, 3 in. Pozidriv shaft, fits no. 2-4 screws No. 2 pt, 4 in. Pozidriv shaft, fits no. 5-10 screws Flathead screwdriver, 2 in. steel shaft, 1/8 in. blade Flathead screwdriver, 4 in. steel shaft, 1/4 in. blade Hex key set, 1.5, 2, 2.5, 3, 4, 5, and 6 mm keys 6 in. long jaw needle nose pliers with side cutters Three open-end wrenches (1/4 in. x 5/16 in., 1/2 in. x 9/16 in., 7/16 in. x 3/8 in.) Snoop-liquid leak detector Teflon tape
Description General Laboratory Tool Kit Part No. 5180-4162
General Laboratory Tool Kit
Wrenches
Description Open end, 1/4 in. and 5/16 in. Open end, 1/2 in. and 7/16 in. Open end, 7/16 in. and 9/16 in. Open end, 7/16 in. and 3/8 in. Adjustable, 12 in., for use with regulators Open end, 9/16 in. and 5/8 in. Open end, 1/2 in. and 9/16 in. Open end, 14 mm Part No. 8710-0510 8710-0806 8710-0803 8710-0972 8710-1712 8720-0010 8720-0025 8710-1924
86
Hardware
Tools
Screwdrivers
Description No. 1 pt, 3 in. Pozidriv shaft, fits no. 2-4 screws No. 2 pt, 4 in. Pozidriv shaft, fits no. 5-10 screws Part No. 8710-0899 8710-0900
Pliers and Tweezers

Description Needle nose, pointed serrated jaws Tweezers, 4 3/4 in. long Truarc pliers Part No. 8710-0004 8710-0007 8710-0018
Tube Cutters and Replacement Blades for SS or Copper Tubing
Description Hi-duty tube cutter (1/8 to 1 1/8 in. od tubing) Hi-duty tube cutter blades Tube cutter (1/8 to 5/8 in. od tubing) Tube cutter blades
Unit 5/pk 5/pk
Part No. 8710-1707 8710-1708 8710-1709 8710-1710
Hex Keys
Description Hex key set, 1.5, 2, 2.5, 3, 4, and 5 mm keys, 1090A (5880/90A) Hex key, 4.0 mm, 15 cm long T-handle Hex key, 3.0 mm, 12 cm long straight-handle Hex key, 2.5 mm, 15 cm long straight-handle Hex key, 1.5 mm, 10 cm long straight-handle Hex key, 9/64 in. 15 cm long T-handle Hex key, 4.0 mm, 10 cm long straight-handle Hex key, 2.5 mm, 10 cm long straight-handle Part No. 8710-0641 8710-2392 8710-2411 8710-2412 8710-2393 8710-2394 5965-0027 5965-0028
87
Hardware
Tools
GC Column Installation Kit

The Capillary Column Installation Kit contains: Diamond-tipped pencil 20x magnifier Ferrule removal tool Septum removal tool Refillable 2 oz. bottle of Snoop
Column Installation Kit
Description Kit with diamond-tipped cutting pencil
Part No. 430-2000
GC Column Rinse Kit

This kit makes solvent rinsing easy! Solvent rinsing removes many contaminants that accumulate in columns. The contaminants are removed by passing solvents through the column. Only bonded and cross-linked phase columns should be rinsed, or permanent damage to the column will result.
Bonded Phase Column Rinse Kit
Description Capillary rinse kit
Part No. 430-3000
Fused Silica Tubing Cutters

We offer two conveniently designed, pencil-shaped tools and a ceramic wafer that allow you to make clean and easy cuts in fused silica, glass and aluminum-clad capillary columns.
Description Diamond-tipped column cutting pencil
Fused Silica Tubing Cutters
Unit
Part No. 420-1000 420-2000
Sapphire column cutting pencil Ceramic wafer column cutter GC Column cutting tool with rotating diamond Replacement diamond blade (for 5183-4620) 4/pk
5181-8836 5183-4620 5183-4621
Column Cutting Tool P/N 5183-4620
88
Hardware
Tools
Miscellaneous Tools
Ferrule Tools
Remove ferrules stuck inside fittings. Screw the stainless steel ferrule tool into the ferrule and pull it out. Use the smaller capillary ferrule tool for capillary column ferrules (0.32 mm id column and smaller). The megabore ferrule tool is used for megabore ferrules. The liner tool can be used to remove polyimide and tubing from inside a megabore liner. The column ferrule installation tool positions a ferrule onto a capillary column for proper installation.
Liner Tool
Description Capillary ferrule tool Megabore ferrule tool Liner tool Column ferrule installation tool
Part No. RFT-2500 RFT-5300 RFT-5100 19251-80680
20x Magnifier
Examine column ends for proper cuts. Jagged, rough, or nonperpendicular cuts negatively affect chromatographic performance.
Column Ferrule Installation Tool P/N 19251-80680
MicroprobesIdeal for Removing GC Septa and Ferrules

Hardened stainless steel probes remove septum and ferrule pieces stuck in fittings. Five different styles give you all of the angles necessary for getting into those hard-to-reach places.
Mirror
Inspect injection ports, the inside of fittings, and other hard-to-reach places.
Description Mirror 20x Magnifier Five Microprobes Part No. 707-0027 430-1020 RMP-5005
Magnifier, Mirror, and Microprobes
Five Microprobes
89
Hardware
Tools Snoop
Leak test most pressurized gas systems. We recommend that leak detecting solutions be used with care where there is any possibility of the fluid being aspirated into gas flow streams supplying the column or detector. This squeezable bottle comes with a Snooper Tube extendable up to 12 inches (30 cm). Electronic leak detectors are preferred (see pages 75 and 80). In their absence, a volatile nonreactive solvent like isopropanol may be used. Use caution to avoid aspiration of materials such as Snoop into the column.
Description Snoop, 8 ounce (236 mL) bottle Snoop, 1 gallon bottle Part No. 9300-0311 460-1002
Injection Port Cleaning Kit

If changing the liner and trimming the column does not remove contamination from your injection port, you probably need to clean and flush your injection port. Three stainless steel brushes and a scraper are the ideal tools for removing sample residue and septum particles. The stainless steel brushes have diameters of 5 mm, 1/4 in. and 3/8 in.
Injection Port Cleaning Kit
FID Cleaning Kit

This easy-to-use maintenance kit improves sensitivity, reduces noise and decreases detector spiking.
Description FID cleaning kit Injection port cleaning kit
FID Cleaning Kit
Part No. 9301-0985 480-0003
12-Piece File Set

This file set scores glass, removes burrs from metal tubing, and cleans threads. This 12-piece file set covers all the laboratory filing needs. The files are 5 1/2 in. long.
Description 12-Piece file set Part No. RSF-1200
90
Hardware
Tools Combination GC Tools
Description Agilent GC Buddy-multi-purpose lab tool for GC Agilent GC Buddy Junior-multi-purpose lab tool for GC Part No. 5182-9765 5183-4789
GC Buddy
Miscellaneous Tools
Description Septum tool, knurled handle Kit, ferrule tool Capillary inlet cleaning wires Swabs 5/pk 100/pk Unit Part No. 450-1000 440-1000 5180-4153 5080-5400
GC Buddy, Jr.
Septum Tool with Knurled Handle
Ferrule Tool
Capillary Inlet Cleaning Wires
91
Chemical Standards
GPC/GFC Standards
Standards
When performing chemical analysis, you can't afford to settle for anything less than the highest standards. That is why Agilent Technologies standards are the perfect solution for you. Every Agilent standard must meet the uncompromising parameters of our ISO-9001 registered quality system. Our quality system means the strictest quality control of incoming raw materials, the meticulous validation of analyte concentrations, homogeneity and stability. So, when it's uncompromising quality you're after, whether it's analytical standards, columns or instrumentation, you can purchase them all from the same reliable source Agilent Technologies.
Gel Permeation Chromatography (GPC) Calibration Standards

Three different polystyrene standard kits are available for organic GPC column calibration. Each kit contains individual monodisperse polymers that cover low, medium, and high molecular weight ranges. Each standard lot is characterized using low-angle laser light scattering, membrane osmometry, and GPC. Typical dispersity values for Agilent's polystyrene standards are 1.05 to 1.07.
GPC Calibration Standards*

Description Low molecular weight (mol wt) range, 10 standards; 162-20 K (0.5 g/ea) Medium mol wt range, 10 standards; 580-3 M (0.5 g/ea) High mol wt range, 10 standards; 300 K-15 M (0.5 g/ea) Part No. 79911-60500 79911-60501 79911-60502
*The exact molecular weights of these standards vary from batch to batch, but each batch is individually certified. The nominal molecular weights of standards are as follows: Low mol wt: 162, 580, 900, 1.4 K, 2.2 K, 3.4 K, 5.1 K, 8.1 K, 12.8 K, 20 K Medium mol wt: 580, 1.5 K, 4 K, 10 K, 27 K, 66 K, 180 K, 460 K, 1.2 M, 3 M High mol wt: 300 K, 460 K, 700 K, 1.1 M, 1.7 M, 2.6 M, 4 M, 6.2 M, 9.5 M, 15 M
GFC Calibration Standards**

Description Polysaccharides: 10 0.2 g standards in mol wt range 180-788 K PEG Standards: 10 individual 0.5 g standards in mol wt range 100-23 K Part No. 1535-4546 1535-4545
**Polysaccharides 10 individual standards 0.2 g ea. mw: 180, 738, 5900, 11800, 22800, 47300, 112000, 212000, 404000, 788000, Polyethylene glycol 10 individual standards, 0.5 g ea. mw: 106, 194, 400, 600, 1000, 2100, 4120, 7100, 11.84 K, 22.8 K
92
Chemical Standards
CE and UV-Vis Standards
CE Standards
Description CE OQ/PV Kit CE IQ Kit Part No. 5063-6520 5063-6514
Note: OQ/PV=Operational Qualification/Performance Verification; IQ=Installation Qualification
Checkout Samples* for UV-Vis Spectrophotometers

Description Holmium oxide glass filter Test sample for UV-Vis (Caffeine solution 10g/mL in water) Part No. 08450-60117 5063-6524
*These are checkout samples only (not certified)
Certified Calibration Standards for UV-Vis Spectrophotometers

In recent years, quality requirements as outlined by ISO 9000, GLP, GMP and NAMAS have assumed increasing importance. As a consequence, the recommendations of pharmacopoeias have become more influential in the pharmaceutical industry. Formal performance verification of UV-Visible spectrophotometers is now essential, but because of the non-availability of appropriate and/or easy-to-use standards, it has been done in ways which do not fully meet regulatory requirements or it has been very time consuming. Agilent Technologies has made performance verification of the Agilent 8453 spectrophotometer much easier by making available sets of liquid standards in snap-open ampoules. The standards can be used with standard open-topped cuvettes, but to reduce the time required and to minimize errors due to cross-contamination from standard to standard, Agilent recommends the use of flow cells for the verification. A hardware kit is available which includes all the items necessary. For reasons of safety in shipment the standards are packaged in two kits.
Standards Kit-I
Standard 60.06 mg/L potassium dichromate 0.01 N sulphuric acid 0.01 N sulphuric acid 12 g/L potassium chloride in water 10 g/L sodium iodide in water 50 g/L sodium nitrite in water 0.02% v/v toluene in hexane Purpose Photometric accuracy at 235, 257, 313 and 350 nm Blank for photometric accuracy test Stray light at 200 nm Stray light at 220 nm Stray light at 340 nm Resolution (ratio of the absorbance maximum at 269 nm to the absorbance minimum at 266 nm) Blank for resolution test
Standards Kit-II
Standard 40 g/L holmium oxide in 10% v/v perchloric acid 10% v/v perchloric acid Purpose Wavelength accuracy in UV and visible ranges Blank for wavelength accuracy
Note: HPLC grade water is recommended as blank for the stray light tests.
Hexane
93
Chemical Standards
HPLC, LC/MS and RI Standards
UV-Vis Standards Kits and Accessories (continued)
Description OQ/PV Standards Kit-I for UV-Vis OQ/PV Standards Kit-II for UV-Vis OQ/PV Hardware kit, contains 2 flow cells (10 mm), tubings and syringes, cell cleaning fluid, Multicell transport adjustment tool Hoya 056 filter, used for performance verification of 8452A Caffeine Standard, IQ Test Sample for UV-Vis, 10 g/mL in water Part No. 5063-6503 5063-6521 5063-6523 89075-23800 08450-60300 5063-6524
Note: OQ/PV=Operational Qualification/Performance Verification; IQ=Installation Qualification
LC-MS Standards
Description Caffeine Standard kit for LC/MS, 0.5, 1.0, 5.0, 25.0, and 50.0 g/mL in water Part No. 8500-6917
General LC Standards
Description Caffeine Standard for HPLC, 5.0, 25.0, 125.0, 250.0, and 500.0 g/mL in water Micro-LC Caffeine Standard kit, 2.0, 4.0, 20.0, 100.0, and 200.0 g/mL in water Fluorescence Detector Calibration Sample, 1 g glycogen Part No. 8500-6762 5065-4420 5063-6597
Qualitative LC Evaluation Standards

Description RI Detector Sample: Contains 0.3% biphenyl, 0.9% o-terphenyl in methanol. One 0.5 mL ampoule Isocratic and Gradient Standards: Contains 0.15% diethylphthalate, 0.01% biphenyl, and 0.03% terphenyl in MeOH (w/w). The gradient standard includes 0.32% dioctyl phthalate as well. Two 0.5 mL ampoules of each. Isocratic Standard: As above. One 0.5 mL ampoule. RI Detector OQ/PV Test sample, 5, 10, 15, 25, and 50 mg/mL glycerin in water Part No. 79877-68701 01080-68702 01080-68704 5064-8220
94
Chemical Standards
HPLC Standards HPLC Column Selectivity Evaluation Standard
This standard is a mix of three PAH materials developed by the National Institute of Standards and Technology (NIST) for the evaluation and comparison of C18 reverse-phase materials. The sample probes the surface of the alkyl chain coating of the silica and measures the degree of polymeric coating. Highly cross-linked phases retain BaP relatively longer. Columns with similar TBN/BaP ratios will elute aromatic non-polar compounds in a very similar fashion, making the array of different C18 materials easier to manage. Each kit comes complete with full NIST certification and documentation on how to use the results. How is the sample useful? TroubleshootingBy logging the performance behavior of new columns, a variable can be eliminated whenever selectivity differences arise. Method developmentUse of the probe can help clarify a C18 phase and simplify column selection in method development.
TBN TBN
PhPh PhPh BaP BaP
Polymeric aTBN/BaP<1.0
Monomeric aTBN/BaP>1.7
Separation of SRM 869 on C18
Standard Reference Material (SRM) 869

Description SRM 869 contains: 0.00112% phenanthrophenanthrene, 0.00032% tetrabenzonaphthalene and 0.00021% benzo(a)pyrene in acetonitrile. Five 1.1 mL ampoules. Part No. 5181-3353
95
Chemical Standards
GC Column Test Standards J&W GC Column Test Standards
Compare your columns performance to the test chromatogram shipped with your J&W column from Agilent. The column test standard contains components that test the column for resolution characteristics, efficiency, and inertness. The test mixes are supplied at a concentration of 250 ng/L in 2 mL vials. Match the phase and column diameter in the chart below to find the test mix for your column. If you would like a gas test mixture for a PLOT column, please call or fax our Technical Support Department.
J&W GC Column Test Standards

Column Description CAM Carbowax20M OV-351 Cyclodex-B CycloSil-B DB -1ht
TM TM
Microbore (0.05 & 0.10 mm ID) Part No.
Capillary (0.18 to 0.32 mm ID) Part No. 200-0021 200-0020 200-0032 200-0025 200-0066 200-0010
Megabore (0.45 & 0.53 mm ID) Part No.
DB-1 DB-1ms DB-5 DB-5ht DB-5ms DB-5.625 DB-17ht DB-17 DB-17ms DB-23 DB-35 DB-35ms DB-200 DB-210 DB-225 DB-502.2 DB-608 DB-624 DB-1301 DB-1701 DB-2887 DB-ALC1
200-0010 200-0010
200-0310 200-0001 200-0310 200-0010 200-0185 200-0056 200-0017
200-0110 200-0110 200-0185 200-0056 200-0117 200-0123 200-0019 200-0019 200-0200 200-0102 200-0225 200-0014 200-0117 200-0113 200-0103 200-0107 200-0110 200-0091
200-0017
200-0317 200-0317 200-0023 200-0019 200-0019 200-0200 200-0002 200-0022 200-0014 200-0017 200-0113 200-0003 200-0007 200-0091
96
Chemical Standards
GC Column Test Standards
J&W Column Test Standards (continued)
Column Description DB-ALC2 DB-Dioxin DB-FFAP DB-HT Sim Dis DB-MTBE DB-Petro DB-TPH DB-VRX DB-WAX DB-WAXetr DB-XLB DX-1 DX-3 DX-4 SE-30 SE-52 SE-54 200-0070 200-0210 200-0210 200-0016 200-0015 200-0370 200-0370 200-0012 200-0061 200-0061 200-0064 200-0010 200-0010 200-0010 200-0010 200-0016
TM
Microbore (0.05 & 0.10 mm ID) Part No.
Capillary (0.18 to 0.32 mm ID) Part No. 200-0092 200-0024 200-0032
Megabore (0.45 & 0.53 mm ID) Part No. 200-0092 200-0132 200-0111
200-0015 200-0070 200-0070 200-0012
Agilent GC Column Test Standards

Our line of Column Test Standards validate the performance of your Agilent columns by checking such components as polarity and inertness. These standards help insure the quality of your chromatograms.
Agilent GC Column Test Standards

Description Megabore Test Sample: 530 m HP-1, HP-5 columns (0.35-1.0 mg/mL) 1-Dodecanol Acenaphthylene n-Pentadecane n-Tridecane 4-Chlorophenol Decylamine n-Tetradecane n-Undecane Ultra Test Mix Sample: 200 m, 250 m and 320 m HP-1 and HP-5 columns (0.35-1.0 mg/mL) 1-Dodecanol Decylamine n-Pentadecane n-Tridecane 4-Chlorophenol Methylcaprate n-Tetradecane n-Undecane Acenaphthylene n-Pentane FFAP Column Test Sample: Used for all Free Fatty Acid Phase columns (0.5-1.8 mg/mL) Acetophenone Heptadecane n-Octanol n-Pentane Butyric acid Hexadecane n-Pentadecane n-Tetradecane Quantity 2 x 1 mL Part No. 8500-6812
1 x 1 mL
5080-8858
1 x 1 mL
8500-6813
97
Chemical Standards
GC and GC/MS Instrument Standards Qualitative Instrument Evaluation Standards
GC, GC/MS, and Headspace Evaluation
Description Capillary Inlet Evaluation Sample (Split Mode): Solution of 1.0% (w/w) of C9, C16, in C14 . Three 0.5 mL ampoules. FID and TCD Sample: This sample is used for the HP 5880, 5890 and 6890 with a FID or TCD. Solution of 0.033% C14, C15, and C16 normal alkanes in hexane. Three 0.5 mL ampoules. Nitrogen-Phosphorus Detector Sample: This sample is used for the HP 5880, 5890 and 6890 with a NPD. Solution of 0.65 ppm azobenzene, 1,000 ppm octadecane, and 1.00 ppm malathion in isooctane (w/v). Three 0.5 mL ampoules. Thermal Conductivity Detector Sample: Solution of 0.33% C14, C15, and C16 normal alkanes in hexane (w/w). Three 0.5 mL ampoules. Part No. 8500-4789 18710-60170 18789-60060
18711-60060 18713-60040 19305-60580
Electron Capture Detector Sample: This sample is used for the HP 5880, 5890 and 6890 with an ECD. Solution of 33 pg/mL (0.033 ppm) (w/v) each of lindane and aldrin in isooctane. Three 0.5 mL ampoules. Flame Photometric Detector Sample: This sample is used for the HP 5880, 5890 and 6890 with an FPD. Solution of 20 ng/L (20 ppm) dodecanethiol and tributylphosphate in isooctane. Three 0.5 mL ampoules.
Atomic Emission Detector Sample: Sample 1: Contains (w/w) 0.07% nitrobenzene, 0.43% n-tridecane, 0.07% 8500-5067 4-fluoroanisole, 0.05% tert-butyldisulfide, 0.08% 1,2,4-trichlorobenzene, 0.05% tetraethyl orthosilicate, 0.05% n-decane (perdeuterated), 4.3% n-dodecane, 0.13% n-tetradecane, 0.07% 1-bromohexane, 4.04% n-octane in isooctane. Sample 2: Contains the same components as Sample 1 plus 0.06% triethylphosphate. Six 0.5 mL ampoules. Three of each sample. ECD Test Sample Lindane, 0.033 ppm in iso-octane, 3 ampoules PFTBA MS Sample kit (0.5 mL ampoule) OQPV Headspace Sample, contains 0.2-0.3% t-butyl disulfide, 1,2-dichlorobenzene, and nitrobenzene in ethanol Capillary Inlet Evaluation Sample, contains 1% n-nonane and 1% n-hexadecane in n-tetradecane 5183-0379 05971-60571 5182-9733 8500-4789
Headspace Sample, contains 0.2-10.0% t-butyl disulfide, 1,2-dichlorobenzene, nitrobenzene, and n-nonane in n-dodecane 8500-4328
Hint: For more GC/MS standards see page 216.
98
Chemical Standards
GC and GC/MS Instrument Standards Qualitative Simulated Distillation Standards
Qualitative Boiling Point Calibration StandardsNot for Quantitative Use
Description Boiling Point Calibration No. 1: Mixture of the following normal hydrocarbons, approximate concentrations % weight/weight: 8.3% n-pentane, 4.4% n-hexane, 4.6% n-heptane, 4.7% octane, 4.8% nonane, 9.7% decane, 4.9% n-undecane, 19.9% n-dodecane, 10.2% n-tetradecane, 5.1% n-pentadecane, 10.3% n-hexadecane, 5.3% n-heptadecane,2.2% n-octadecane, 1.3% n-eicosane, 1.3% n-tetracosane, 0.9% n-octacosane, 0.9% n-dotriacontane, 0.9% n-hexatricontane, 0.9% n-tetracontane. For use with ASTM method D 2887. Six 1 mL ampoules. Part No. 5080-8716
Low Boiling Point Calibration No. 220: Mixture of the following normal hydrocarbons, approximate concentrations 5080-8768 % weight/weight: 8.8% n-pentane, 4.7% n-hexane, 4.8% n-heptane, 4.9% n-octane, 5.1% n-nonane, 10.3% n-decane, 5.2% n-undecane, 21.1% n-dodecane, 10.8% n-tetradecane, 5.4% n-pentadecane, 10.9% n-hexadecane, 5.5% n-heptadecane,2.4% n-octadecane. Six 1 mL ampoules.
General Catography
Boiling Point Calibration No. 320: Mixture of the following components, approximate concentrations % weight/weight: 6.9% n-pentane, 4.8% 2-methyl pentane, 3.6% n-hexane, 3.7% 2,4-dimethyl pentane, 3.8% n-heptane, 4.8% toluene, 3.9%n-octane, 4.8% p-xylene, 4.8% n-propyl benzene, 8.1% n-decane, 4.7% n-butyl benzene, 16.5% n-dodecane, 4.3% n-tridecane, 8.4% n-tetradecane, 4.2% n-pentadecane, 8.5% n-hexadecane, 4.3% n-heptadecane. Six 1 mL ampoules. Internal Standard Mixture 20: Mixture of the following components, approximate concentrations % weight/weight: 33.3% n-tetradecane, 13.3% n-pentadecane, 26.7% n-hexadecane, 26.7% n-heptadecane. For use with proposed method for boiling point distribution of crude petroleum. ASTM Standards, 1976, Vol 25, pp 716-722, one 25 mL bottle.
5080-8769
5080-8723
Petrochemical
Qualitative Petrochemical Standards
Description Alcohol in Gasoline: Test mixture containing approximately 1% each of ethanol, isopropanol, n-propanol, sec-butanol, isobutyl alcohol, tert-amyl alcohol, and n-butanol; 2% each of methanol and tert-butyl alcohol; and 3% MTBE in gasoline. Three sealed 0.5 mL ampoules. Natural Gas: Approximate concentrations % volume/volume: 69% methane, 6% nitrogen, 1% carbon dioxide, 0.5% oxygen, 9% ethane, 1% n-pentane, 6% propane, 3% isobutane, 3% n-butane, 1% iso-pentane and 0.5% hexanes. One pressurized aerosol-type container (370 mL at 25 psig). Transformer Gas: Approximate composition: 0.1% each of carbon dioxide, propylene, hydrogen, carbon monoxide, propane, ethane, methane, ethylene, acetylene, butene-1, and 99% nitrogen. One pressurized aerosol-type container (370 mL at 25 psig). Refinery Gas: Approximate concentrations % volume/volume: 15% hydrogen, 5% propane, 1% propylene, 10% iso-butane, 2% iso-pentane, 1% n-pentane, 15% nitrogen, 5% methane, 5% n-butane, 10% 1-butene, 5% trans-2-butene, 5% cis-2-butene, 5% carbon dioxide, 5% carbon monoxide, 1% ethylene, and 10% ethane. One pressurized aerosol-type container (370 mL at 25 psig). Reference Gas Oil No. 1 Batch 2: Multiple component mixture of hydrocarbons from petroleum fractions. Used for performance verification of the Agilent simulated distillation analyzer, Option 840. The analyzer performs analysis of petroleum fractions according to ASTM Method D 2887. Three 1 mL ampoules. Part No. 18900-60640
5080-8756
5080-8759
5080-8755
5060-9086
99
Chemical Standards
GC and GC/MS Instrument Standards Qualitative Standards
Miscellaneous Qualitative Standards
Description Valve Checkout: Mixture of 0.5% each of methane, pentane, and hexane in helium (98.5%). For checking backflush, gas sampling, column isolation, and other valve systems. One pressurized aerosol-type container (370 mL at 25 psig). Nickel Catalyst: Mixture of 105 ppm each of methane, carbon monoxide, and carbon dioxide in helium. One pressurized aerosol-type container (370 mL at 25 psig). Nickel Catalyst Refill MIDI System Calibration Standard: Quantitative calibration standard for the Microbial ID System. Fatty acid methyl esters w/v%. 0.01% n-C9:0, 0.02% n-C10:0, 0.01% n-C11:0, 0.02% C12:0, 0.01% C13:0, 0.02% C14:0, 0.01% n-C15:0, 0.02% n-C16:0, 0.01% n-C17:0, 0.02% n-C18:0, 0.01% n-C19:0, 0.02% n-C20:0, 0.005% C10:0 2OH, 0.0025% C10:0 3OH, 0.005% C14:0 2OH, 0.0025% C14:0 3OH and 0.005% C16:0 2OH. Four 2 mL ampoules. Hint: For a complete selection of single and multi-element reference standards for ICP-MS, refer to the ICP-MS section of this catalog on pages 699-700. Part No. 5080-8765 19354-60510 5080-8761 19298-60500
Gas Analyzer Accessories and Standards

Accessories
Description Regulator for Calib Mix Cylinders, 1 ea, Regulator type: Single stage, with swivel connector, Fittings: 1/4 in. female NPT swivel to cylinder, 1/16 in. SS compression fitting to GC, Pressure gauge range: 0 to 400 psi, Flow range: Dedicated 20 mL/min 5% Carry Case for Calib Mix Cylinders, 1 ea, Dimensions: 13.5 in. x 10 in. x 4 3/8 in., Material of construction: Outer shell: High density polyethylene, Inner foam: Polyester Urethane, Holds: 2 cylinders, 2 regulators, and 2 SS pieces of gas sampling tubing Gas Sampling Tubing 1/16 in. SS with Fttgs, Specifications: 1/16 in. SS tubing, 610 mm long, 0.038 in. id with two 1/16 in. SS Swagelok nuts, front ferrules, and back ferrules Part No. 5184-3539
5184-3540
5185-5817
Gas Standard Cylinders*

Description Universal Calib Mix Cylinders, Box of 2, 0.10% Helium, 0.05% Neon, 0.10% Hydrogen, 0.05% Oxygen, 0.10% Nitrogen, Balance 99.05% Methane, 0.05% Ethane, 0.05% Ethylene, 0.05% Carbon Dioxide, 0.10% Carbon Monoxide, 0.05% Acetylene, 0.05% Propane, 0.05% Methyl Acetylene, 0.05% n-Butane, 0.05% n-Hexane, 0.05% n-Heptane NGA (Natural Gas Analyzer) Calib Mix Cylinders, Box of 2, 5.17% Nitrogen, Balance 72.24% Methane, 8.997% Ethane, 1.495% Carbon Dioxide, 6.001% Propane, 2.999% Isobutane, 2.000% n-Butane, 0.50% Isopentane, 0.50% n-Pentane, 0.10% n-Hexane RGA (Refinery Gas Analyzer) Calib Mix Cylinders, Box of 2 Part No. 5184-3541
5184-3542
5184-3543
*Cylinder specifications: Pressure (when full): 240 psi, Volume: 8.5 liters, Temperature range: 0F to 450F, Diameter: 2.75 in., Height (w/valve): 7.25 in., Tank material: Carbon steel, Concentrations: 5% accuracy, w/w.
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Chemical Standards
GC and GC/MS Instrument Standards
Gas Standard Cylinders (continued)
Description Univ/NGA Calib Mix Cylinder Combo Box** Univ/RGA Calib Mix Cylinder Combo Box** Part No. 5184-3544 5184-3545
Gas Standard Kits***

Description Universal Calibration Kit, 1 ea, with two Universal mix cylinders NGA Calibration Kit, 1 ea, with one Universal and one NGA mix cylinder RGA Calibration Kit, 1 ea, with one Universal and one RGA mix cylinder Part No. 5184-3546 5184-3547 5184-3548
**Combo boxes contain: One box of 2 calibration mix cylinders, containing one cylinder of Universal mix, and the other is either an NGA or RGA cylinder. ***Calibration kits contain: One box of 2 calibration mix cylinders, 2 regulators, 2 pieces of SS 1/16 in. gas sampling tubing, and 1 carry case, all packaged separately.
Regulator, Carry Case and Mix Cylinders
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Integrators, Integrator Supplies, and Cables

Ink Cartridges/Integrator Paper

We Can Handle Your Data Handling Needs Perfectly, Too.
At Agilent Technologies, our goal is to provide high quality columns and supplies that are the perfect fit for your instrumentation. In between our rigorous designing, manufacturing and testing, we have also taken the time to listen. We understand that your laboratory needs encompass far more than simply columns and consumables. Which is why we offer our established line of integrators, data handling supplies and cables. Information generation and its presentation has never been more important. Agilent understands that data handling supplies are indeed also laboratory supplies. So it is essential that you never run out of them at a crucial moment. We provide a means to purchase ink cartridges and integrator supplies just as conveniently as you can order our other laboratory products.
Integrator Cartridges
Our replacement print cartridges are the perfect fit for your integrator. They have been designed and tested specifically for use with our Integrators, and provide the quality you've come to expect.
Agilent Integrator Ink Cartridges and Printheads

Description Agilent 3397A, 3396 Series III, 3396A and B, 3395, 3394 Printhead for 3388A and 5880A Part No. 5181-1220 19350-60540
Helpful Hint: Agilent Integrator Cartridges have an expected lifetime of 18 months, 6 months after installation.
Integrator Paper and Supplies

Paper and Supplies for the Agilent 3397A, 3396 Series III, and 3396A/B/95/94
Description Z-fold paper stand, acrylic Z-fold paper, sequentially numbered, 8.5 x 11 in. InkJet Perforated paper, 8.5 x 11 in., InkJet Perforated paper, sequentially numbered, 8.5 x 11 in., Inkjet Perforated paper, A4 size, InkJet Replacement paper roll rod Replacement paper roll brackets 2/pk 5 pk/box, 2,500 sheets 4 rolls/pk 4 rolls/pk 4 rolls/pk Unit Part No. 5062-3560 5062-3561 5181-1219 5183-2009 5181-1255 03394-20500 03396-40050
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Integrator Paper/Integrators and Accessories
Integrator Paper and Supplies

Thermal Paper for the Agilent 3390/92/93
Description Thermal Printing Paper, blue print, 4.5 in. wide Unit 4 rolls/pk Part No. 5080-8800
Thermal Paper for the Agilent 338X/5830/3370

Description Z-fold paper for 3388A and 5880A, blue print 8.5 in. wide Z-fold paper for 3388A and 5880A, black print, 8.5 in. wide Z-fold paper for 3380/85A Integrators and 5830/40A terminals, blue print, 8.5 in. wide Unit 8 pks of 200 sheets each 8 pks of 200 sheets each 8 pks of 200 sheets each Part No. 5080-8778 9270-0658
5080-8735
Helpful Hint: You can use sequentially numbered paper to better track your analyses. Simply record the numbers in your lab notebook for later reference.
Integrators and Accessories

Integrator Features
Integrator Agilent 3396 Series III Integrator Features BASIC programming Reintegration/replot Negative peak integration Industry standard RS-232 communications port Offers 256 kb RAM Multiple-level calibration Instrument Control (INET)
Agilent 3395B Series III Integrator Stores multiple data files in RAM Multiple-level calibrations Reintegration/replot for up to 63 levels 100% memory backup for Offers 128 kb RAM for power failure protection storing up to 20 hrs of chromatographic data at 4 Hz.
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Integrators and Accessories/Integrator Cables Integrators and Accessories
Description 3396 Series III Integrator with BASIC programming 3395 Series III Integrator with 128 kb of RAM, general purpose signal, and remote cables
Agilent Technologies 3396 Series III
Part No. 3396C 3395B G2101A G2100A G2104A G2105A
GC Dual-channel Appak-Series III, 5890 and 6890 GC control software for the 3396 series III integrator* Integrator Starter Kit (8.0 MB data storage card, Z-fold paper stand, Z-fold paper (500 sheets), and three ink cartridges) Integrator Data Storage card, 8 MB Integrator Data Storage card, 16 MB
*Requires Agilent 5890 GC communications board, option 552 or accessory 19242B
Integrator Cables
Agilent 3396 Series III Cable Sets
Description Series III Integrator General Purpose cables, signal/remote cable pair includes 35900-60630 and 03396-61031 INET interface for Agilent integrators, provides INET communications and dual-channel output, includes 1 INET cable Part No. G2108A G1553A
Cables to Connect any Agilent 3397A or 3396/95 Series III Integrator

Cable Type Instrument 6890 Series GC (non-INET) 5890 GC (non-INET) 1100 Series LC Analog G1530-60570 03396-61010 35900-60610 03394-60560 35900-60750 03396-61010 03396-60560 1090 Series LC 35900-60600 03396-60650 03396-60560 Remote Sample
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Integrator Cables Cables to Connect any Agilent 3397A or 3396/95 Series III Integrator (continued)
Cable Type Instrument 1050 Series LC Analog 35900-60750 03396-61010 03396-60560 1040 diode array detector 1046A fluorescence detector 7673 automatic sampler (non-INET) All other instruments (general-purpose use) 35900-60630 03394-60540 03396-60500 35900-60600 03396-60650 35900-60750 03394-60600 Remote Sample
03396-60550 03396-60560
Cables to Connect any Agilent 3394/95A and 3396A/B Integrator

Cable Type Remote 03396-61010 03394-60560 1090 Series LC 35900-60600 35900-60710 03396-60650 03396-60560 1050 Series LC 35900-60750 01046-60210 03396-60560 1040 diode array detector 1046A fluorescence detector 7673 automatic sampler General Purpose 35900-60630 35900-60920(5m) 35900-60900(5m) 03394-60540 03396-60500 www.agilent.com/chem 35900-60610 03396-60650 35900-60750 01046-60210 03395-60540 03396-60560 Instrument 6890 Series GC 5890 GC Analog G1530-60570 03396-61020 35900-60610 Sample
Helpful Hint: Need help selecting replacement cables? Call us. We'd be happy to assist you.
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HPIB/GPIB/Instrument Interface Cables HPIB and GPIB Cables
Description GPIB Interface (82341C) PCI GPIB Card for Win95/98/NT HPIB Cable 2m (10833B) General Purpose Cable GPIO-Open End GPIB Interface for MS Windows and DOS HPIB Cable, 8 meter (10833G) Cable, 6890 to PC 9F/9F RS232 Crossover Ethertwist Cable, 10 ft Adapter, extends GPIB connector (10834A) Part No. G1680-63711 G1680-63715 10833B-2310 G1103-61611 G1680-63710 10833G-2310 G1530-60600 5183-4649 10834A-2310
Instrument Interface Cables

Description Signal cable, general purpose analog output cable assembly, spade lugs/6 pins External event control, cable, 8 pins/spade lugs Remote start cable for general use with lug 6890 to PC via RS 232-C, 9 female/25 male user card cable General purpose binary-coded decimals cable with spade lugs Automatic liquid sampler; remote start/stop cable, 2 m 9 male/9 female HPIB cable, 2 m HPIL cable, 5 m Remote Y Cable, G1512/5890A to 3396C Remote Cable to 5890 Part No. G1530-60560 G1530-60590 35900-60670 G1530-60610 G1530-60630 G1530-60930 10833B-2310 82167-60003 G1512-60530 35900-60700
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Books and Learning Software Books, Learning Software, and CDs

PerfectFit: We Wrote the Book. Agilent Technologies offers texts, computer-based tutorials, quick reference guides, and owner manuals. For a complete list of instrument manuals and applications for CE, GC, LC, UV-Vis, Integrators, AED, and ICP-MSplease go to our website at www.agilent.com/chem. There you will find a complete up-to-date list of literature and manuals.
CBT Software
The Basics of Chromatography at Your Fingertips
Training programs for GC and LC instrument operators Take advantage of these new, self-paced tutorial programs designed for novice GC and LC instrument operators. With these informative, interactive, computer-based training programs, your laboratory staff will learn chromatography basics quickly and easily. Interactive software for maximum involvement in learning the fundamentals of chromatography. Chromatographic simulation and testing scenarios save hours of trial and error experimentation. This feature shows the results of changes on chromatograms when experimental parameters are varied. Progress reports record user's study history and document exam results for proof-of-training in audit trails. Customization by adding graphics and/or text unique to your own laboratory applications. Ability to learn at your own pace without an instructor. Available anytime at your convenience; at your home, lab or office; on your computer. Self-testing and rapid feedback ensure comprehension displaying test results and directing users to subjects needing further study. Search and find any word or phrase of text. Hot Words provide instant on-screen definitions of many important chromatographic terms. A glossary offers comprehensive listings and a book mark allows easy later return.
CBT Software
Description Introduction to Gas Chromatography Introduction to Gas Chromatography, Spanish Version Introduction to HPLC Method Development in HPLC HPLC Equipment Separation Modes of HPLC Identification and Quantitation Techniques in HPLC Troubleshooting HPLC Part No. 5183-4654 5183-4727 5183-4655 5183-4657 5183-4656 5183-4659 5183-4660 5183-4658
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Books and Learning Software

Retention Time Locking (RTL) Software for the Agilent 6890 GC System
Retention time locking (RTL) software is an easy to use software product which allows users to match retention times from one capillary Agilent 6890 GC to another Agilent 6890 GC System. RTL software is integrated into the GC ChemStation. Requires Agilent Multi-technique GC ChemStation revision A.05.02 and the 6890 GC with EPC inlet.
RTL Software for the Agilent 6890 GC System

Description Retention Time Locking Software
Philip Stremple
Part No. G2080AA
Books
General Chromatography Books
Description Handbook of Chemistry and Physics, Edited by C. Weast (CRC Press, latest edition) System Validation of Computerized Analysis Systems by L. Huber Validation and Qualification in Analytical Laboratories by L. Huber Part No. 9320-5798 5959-3879 5965-0036
Gas Chromatography Books

Description Detectors for Gas ChromatographyA Practical Primer by R. Buffington and M. Wilson (Agilent Technologies, 1987)
Dean Rood
Part No. 5958-9433 5183-2008 5958-9468 5182-0842 5950-3562 9320-5710 900-1010 900-1013
Gas Chromatography by I. Fowlis, 2nd edition, (John Wiley & Sons, 1995) GC InletsAn Introduction by M. S. Klee (Agilent Technologies, 1990) Electronic Pressure Control, Primer with Flocalc Disk (Agilent Technologies) High-Resolution Gas Chromatography, 3rd Edition Edited by K. J. Hyver, guest author P. Sandra (Agilent Technologies, 1989) Modern Practice of Gas Chromatography Edited by R. L. Grob (John Wiley & Sons, 1995) Analytical Gas Chromatography, 2nd Ed., W.G. Jennings, E. Mittlefehldt, P. Stremple A Practical Guide to the Care, Maintenance and Troubleshooting of Capillary GC Systems, 3rd Ed., D. Rood
Walt Jennings, Cofounder, J & W Scientific
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Books and Learning Software

Liquid Chromatography Books
Description Introduction to Modern Liquid Chromatography, 2nd Edition by L. R. Snyder and J. J. Kirkland (John Wiley & Sons, 1979) Maintaining and Troubleshooting HPLC Systems by D. J. Runser (J. Wiley Interscience, 1981) Practical HPLC Methodology and Applications by Brian A. Bidlingmeyer (John Wiley & Sons, 1993) Practical HPLC Method Development, 2nd edition by L. R. Snyder, J. J. Kirkland, and Joseph Glajch (John Wiley & Sons, 1997) Part No. 9320-5713
9320-5790 5183-2006 5183-2005
Educational Primers
Description High Performance Capillary ElectrophoresisAn Introduction Good Laboratory Practice Pub. No. 5968-9963E 5968-6193E
Helpful Hint: A variety of educational primers and literature is available from Agilent at no charge. Simply call your local sales office, authorized distributor, or consult our website for more information.
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Sample Preparation
Table of Contents
Sample Preparation Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .111 Zorbax SPE Products Zorbax SPE Cartridges and 96-Well Plates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .112 Zorbax SPE Formats . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .113 Zorbax C18 SPE Products . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .114115 Zorbax C18 EC SPE Products . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .116117 Zorbax C8 SPE Products . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .118 Zorbax Silica SPE Products . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .119 AccuBONDII SPE Products AccuBONDII SPE Cartridges . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .120 AccuBONDII Reversed Phase (Non-Polar) SPE Products . . . . . . . . . . . . . . . . . . .120122 AccuBONDII Normal Phase (Non-Polar) SPE Products . . . . . . . . . . . . . . . . . . . . . . . .122 AccuBONDII Normal Phase (Polar) SPE Products . . . . . . . . . . . . . . . . . . . . . . . .123124 AccuBONDII Ion Exchange SPE Products . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .124125 Comparative Herbicide Extractions Using AccuBONDII SPE Products . . . . . . . . . . . . .126 AccuBONDII Non-Silica SPE Products . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .127128 AccuBONDII Cartridge Starter Kits and Bulk SPE Sorbents . . . . . . . . . . . . . . . . . . . . .129
Sample Preparation
EvidexII SPE Products EvidexII SPE Products . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .130131 Manifolds and Accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .132 Vacuum Manifolds . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .133 Adapters and Replacement Parts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .134 Vacuum Manifolds for 96-Well Plates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .135137 AccuBONDII Empty Reservoirs and Frits . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .138 CombiZorb Scavengers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .139 CombiZorb Physical Data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .140141 Sample Filtration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .142147
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SPE Solutions for Your Challenging Sample Preparation Problems

Effective sample preparation can make the difference between a definitive measurement and a record of impurity-driven interferences. If samples are taken incorrectly or the sample preparation protocols do not provide good recovery or interference removal, then all the subsequent downstream steps can be negated. Agilent Technologies provides several sample preparation product lines that can help you to solve some of those challenging sample preparation problems. All of Agilents sample preparation products are manufactured under a quality program that conforms to ISO-9000 to ensure that these products meet the consistency and uniformity that you require in your everyday work.
Solid-Phase Extraction
Solid-Phase Extraction (SPE) is a technique for the cleanup and concentration of analytes from various matrices. SPE improves and simplifies separations, increases chromatography column lifetime, and improves detection limits. The following information outlines the primary steps in the application of SPE to liquid samples.
Sample Preparation
Agilent Technologies covers the full range of modern SPE requirementscartridges, 96-well plates and accessories. Zorbax SPE and AccuBONDII are SPE product families. Whatever your SPE requirements, think Agilent first.
Combinatorial Chemistry Scavengers

These products resemble SPE products except that they have chemical functionality that permits these solid phases to react with and remove undesirable reaction by-products and excess reactants from chemical reactions and combinatorial compound library syntheses. Agilent provides a large number of scavengers that will remove just about any interference encountered.
Sample Filtration
Membrane sample filters are used to clarify any samples that need further analysis or use where particulate matter may cause a problem. They are most often used for the filtration of HPLC samples to prevent damage to the injection valve or to the HPLC column inlet. Agilent provides a range of standard and economy syringe filters conveniently housed in inert polymeric housings for easy use and disposal.
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SPE
Zorbax SPE Products
Zorbax SPE Cartridges and 96-Well Plates the High Performance Choice
ZORBAX is the name you have come to know and trust for HPLC columns. Now Agilent adds Zorbax SPE productsproducts that set the same high standard to meet your most exacting sample preparation requirements. Zorbax SPE products, based on proven ZORBAX spherical base silica technology, bring you these advantages: Best reproducibility batch-to-batch, tube-to-tube, plate-to-plate Unique spherical particles that reduce pressure drop, dramatically increase flow reproducibility compared to competitive irregular SPE particles, that may contain fines and may allow replacement of vacuum manifolds with gravity-driven flow Lowest extractablespre-washed cartridges, well plates, frits, and packing materials Triple-laminated bag that ensures cleanliness and excludes oxygen and moisture before use Ultra high purity base silica, resulting in low trace metals, low acidity, and no strong irreversible adsorption of basic compounds, enhancing separation sensitivity High, repeatable recovery, leading to the most accurate quantitation with little loss of analyte on the sorbent Flow-tested to confirm fewer fines and narrow size distribution compared to sorbents from other sources, for more predictable packing density and reproducible flow characteristics Certificate of performance, based on the most rigid chromatographic tests in the industry, supplied with each lot helping you to standardize your separations Wide variety of productscartridges, 96-well plates (both fixed and flexible), and complete line of accessories, including vacuum manifolds and collection plates
Sample Preparation
ZORBAX Spherical Particles vs Competitive Irregular SPE Particles
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SPE
Zorbax SPE Products
Agilent Technologies Offers a Choice of SPE Formats from Cartridges to 96-Well SPE Plates
SPE Cartridgesthe traditional syringe barrel design cartridges in sizes from 1 mL volume to 6 mL volume packed with 25 mg to 1000 mg of packing per cartridge. The cartridges are used with standard vacuum manifolds.
SPE Cartridges
96-Well SPE Fixed Platesdesigned for high throughput SPE processing, these flowthrough design plates consist of 96 small SPE cartridges in a single polypropylene housing that fits in most standard 96-well plate vacuum manifolds. Each well has a total volume of 2 mL and weights of 25, 50 or 100 mg/well.
Sample Preparation
96-Well Fixed Plate
96-Well SPE Flexible Platesin this design, a flexible well base with 96 individual holes accommodates individual cartridges comparable in size and packing volumes to the wells in the fixed 96-well plates. You can prepare your own custom designed plates by placing individual cartridges in the base plate holes. Individual cartridges come in packs of 100. The flow-through flexible plates are useful for method development since they allow you to test a variety of phases, masses and volumes to optimize their choice of SPE conditions. The small SPE flexible cartridges can also be used with standard vacuum manifold system using a small Luer adapter. Each flexible cartridge has a total volume of 1 or 2 mL and weights of 25, 50 or 100 mg.
Flexible 96-Well Plate Configuration
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SPE
Zorbax SPE Products
Zorbax C18 SPE Products

Reversed phase mode Octadecylsilane (ODS, C18) Monomeric Bonding (11.1% carbon loading) Non-endcapped, enhanced polar interactions relative to endcapped (EC) version Spherical silica, reduced pressure, more consistent flow 80 Angstrom average pore size; 50 m average particle diameter Recommended as general purpose SPE phase for both polar and non-polar analytes Retention mechanisms: - Non-polar - Polar - Cation exchange by virtue of ionized silanols
Structure of C18 octadecylsilane (ODS), covalently bonded to the surface of a silica particle
Sample Preparation
Description
C18 Cartridges
Unit 100/pk 100/pk 100/pk 50/pk 50/pk 30/pk 30/pk
Part No. 5184-3588 5184-3589 5184-3590 5184-3591 5184-3592 5184-3593 5184-3594
25 mg, 1 mL 50 mg, 1 mL 100 mg, 1 mL 200 mg, 3 mL 500 mg, 3 mL 500 mg, 6 mL 1000 mg, 6 mL
C18 96-Well SPE Plates
25 mg/well 25 mg/well 50 mg/well 50 mg/well 100 mg/well 100 mg/well

C18 Flexible Plate Cartridges*
5185-5721 5/pk 5/pk 5/pk 5185-5722 5185-5723 5185-5724 5185-5725 5185-5726
25 mg, 1 mL 50 mg, 1 mL 100 mg, 1 mL 100 mg, 2 mL
100/pk 100/pk 100/pk 100/pk
5185-5745 5185-5746 5185-5747 5185-5769
*Requires base plate (P/N 5185-5787) for 96-well operation
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SPE
Zorbax SPE Prodcuts

Application Example
Analysis of Naproxen in Serum: Preparation with a Zorbax SPE C18 Cartridge Solid Phase Extraction Method CARTRIDGE: PRE-TREAT: LOAD: WASH: ELUTE: INTERNAL STANDARD: RECOVERY: 100 mg, 1 mL Zorbax C18 (P/N 5184-3590, 100/pk) 1% Acetic acid in methanol, deionized water 1.0 mL sample 4% Isopropyl alcohol in 100 mM formic acid, deionized water 50% Acetonitrile in 40 mM ammonium acetate Nitrobenzene in methanol
Comparison of Naproxen-spiked serum before (A) and after (B) extraction using the Zorbax SPE C18 cartridge and analysis by HPLC. Note substantial reduction of protein extractables, providing better resolution and quantitation of the drug. HPLC column lifetime was extended due to cleaner sample injections. HPLC Column used: ZORBAX SB-C8, 4.6 x 150 mm (P/N: 863953-906)
Sample Preparation
HIGH LOAD (10 g): X = 99.25%, n = 3 LOW LOAD (2 g): X = 102.2%, n = 3
Application Example
Analysis of Ranitidine in Serum: Preparation with a Zorbax SPE C18 Cartridge Solid Phase Extraction Method CARTRIDGE: PRE-TREAT: LOAD: WASH: ELUTE: INTERNAL STANDARD: RECOVERY: 100 mg, 1 mL Zorbax C18 (P/N 5184-3590, 100/pk) Methanol, deionized water 1.0 mL sample Deionized water Methanol Prednisolone in deionized water X = 100%, n = 3
Comparison of Ranitidine-spiked serum before (A) and after (B) extraction using the Zorbax SPE C18 cartridge and analysis by HPLC. SPE can help to lower interferences and provide enhanced resolution for routine drug testing. HPLC Column Used: ZORBAX Bonus RP, 4.6 x 150 mm (P/N: 883688-904)
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SPE
Zorbax SPE Products
Zorbax C18 EC SPE Products

Reversed phase mode Octadecylsilane (ODS, C18) Monomeric Bonding (14.8% carbon loading) Endcapped, reduced polar interactions relative to non-endcapped version Spherical silica, reduced pressure, more consistent flow 80 Angstrom average pore size; 50 m average particle diameter Recommended for non-polar analytes Retention mechanisms: - Non-polar
Structure of C18 silane and trimethyl silyl endcapping group, covalently bonded to the surface of a silica particle
Description
C18 EC Cartridges
Part No. 5184-3595 5184-3596 5184-3597 5184-3598 5184-3599 5184-3600 5185-5701
25 mg, 1 mL
Sample Preparation
50 mg, 1 mL 100 mg, 1 mL 200 mg, 3 mL 500 mg, 3 mL 500 mg, 6 mL 1000 mg, 6 mL
C18 EC 96-Well Plates

C18 EC Flexible Plate Cartridges*
5185-5727 5/pk 5/pk 5/pk 5185-5728 5185-5729 5185-5730 5185-5731 5185-5732
100/pk 100/pk 100/pk 100/pk
5185-5748 5185-5749 5185-5750 5185-5770
*Requires base plate (P/N 5185-5787) for 96-well operation
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SPE
Zorbax SPE Products
Zorbax C18 EC SPE Products

Application Example
Analysis of Clozapine and Metabolites in Plasma: Preparation with a Zorbax SPE C18 EC Cartridge* Solid Phase Extraction Method CARTRIDGE: PRE-TREAT: LOAD: WASH: ELUTE: INTERNAL STANDARD: RECOVERY: 100 mg, 1 mL Zorbax C18 EC (P/N 5184-3597, 100/pk) Methanol, deionized water Plasma, flurazepam, deionized water Deionized water, methanol/water Methanol Flurazepam
Analysis of clozapine and its metabolites using the Zorbax SPE C18 EC cartridge.
Sample Preparation
Clozapine (200 g/mL): X = 103%, n = 10 LOW LOAD (2 g): Norclozapine (200 g/mL) X = 111, n = 10
* Analysis of Clozapine: Zorbax SPE C18 EC Cartridge courtesy of Dr. David Freeman, Dept. of Pharmacology and Toxicology, J. P. Robarts Research Institute, London, Ontario, Canada
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SPE
Zorbax SPE Products

Reversed phase mode Octylsilane (ODS, C8) Monomeric Bonding (8.3% carbon loading) Non-endcapped, polar interactions Spherical silica, reduced pressure, more consistent flow 80 Angstrom average pore size; 50-m average particle diameter Recommended as a general purpose sorbent for both polar and non-polar analytes less retentive compared to Zorbax C18 (SPE) Retention mechanisms: - Non-polar - Polar - Cation exchange by virtue of ionized silanols
Structure of C8 octyl silane, covalently bonded to the surface of a silica particle
Description
Part No. 5184-3574 5184-3575 5184-3576 5184-3577 5184-3578 5184-3579 5184-3580
Sample Preparation
C8 Cartridges
96-Well SPE Plates, 5/pk
C8 96-Well SPE Plates

C8 Flexible Plate Cartridges*
5185-5709 5/pk 5/pk 5/pk 5185-5710 5185-5711 5185-5712 5185-5713 5185-5714
C8, 25 mg, 1 mL C8, 50 mg, 1 mL C8, 100 mg, 1 mL C8, 100 mg, 2 mL
100/pk 100/pk 100/pk 100/pk
5185-5739 5185-5740 5185-5741 5185-5796
*Requires base plate (P/N 5185-5787) for 96-well operation.
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SPE
Zorbax SPE Products
Zorbax Silica SPE Products

Normal phase (adsorption) mode Unbonded, activated silica gel adsorbent Polar interactions with silanols via hydrogen bonding Recommended as adsorbent for polar analytes in non-polar matrices Retention mechanisms: - Non-polar matrix, polar analytepolar retention mechanism - Aqueous matrix, cationic analyte, intermediate pHcation exchange retention mechanism
Chemical structure of silanol groups on the surface of a silica particle
Description
Silica Cartridges
Part No. 5185-5702 5185-5703 5185-5704
Silica 96-Well SPE Plates
Sample Preparation
5185-5705 5185-5706 5185-5707 5185-5708

Silica Flexible Plate Cartridges*
5185-5733 5/pk 5/pk 5/pk 5185-5734 5185-5735 5185-5736 5185-5737 5185-5738
100/pk 100/pk 100/pk 100/pk
5185-5751 5185-5752 5185-5753 5185-5774
*Requires base plate (P/N 5185-5787) for 96-well operation.
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SPE
AccuBOND" SPE Products
AccuBONDII SPE Cartridges A Versatile Sample Preparation Family

The AccuBONDII name on SPE cartridges indicates high quality in a wide selection of phases for use in routine analyses that call for consistent results with higher sample volumes. Based on high purity, irregularly shaped silica packing, AccuBONDII cartridges bring you these valuable characteristics: High quality, low extractables for dependable separations Solvent pre-washed sorbents, frits, and cartridge bodies Wide variety of phases Larger mass cartridges for extraction of environmental samples Suitable for general SPE method development Specific phases for routine extractions of drugs of abuse from biological fluids Attractive pricing for routine, high volume laboratory use
Sample Preparation
AccuBONDII Reversed Phase (Non-polar) SPE Products

Reversed phase sorbents are non-polar, and used to retain (extract) non-polar analytes. For reversed phase sorbents, solvent strength increases as the solvent becomes more non-polar.
AccuBONDII C18 Products

AccuBONDII C18 products are based on bonded, reversed phase (octadecylsilane), irregular silica gel (silica) particles. This non-polar, non-endcapped sorbent provides additional polar interactions associated with surface silanol groups. It also enhances retention of basic compounds compared with the corresponding endcapped sorbent. It is recommended as a general purpose SPE phase for both polar and non-polar analytes.
Structure of octadecyl silane, covalently bonded to the surface of a silica particle
Description
Octadecylsilane (ODS) (C18)
Unit 100/pk 50/pk 50/pk 30/pk 30/pk
Part No. 188-1310 188-1320 188-1350 188-1356 188-1360
100 mg, 1 mL 200 mg, 3 mL 500 mg, 3 mL 500 mg, 6 mL 1000 mg, 6 mL
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SPE
AccuBOND" SPE Products AccuBONDII Octyl (C8) SPE Products
AccuBONDII C8 products are based on bonded, reversed phase (octylsilane), irregular silica gel (silica) particles. This non-polar, non-endcapped sorbent is recommended as a general purpose SPE phase for non-polar analytes. It is slightly less retentive than AccuBONDII C18 products. For basic analytes, octyl sorbents often can increase the extraction efficiency and enhance their purity.
Structure of octyl (C8) silane, covalently bonded to the surface of a silica particle
Description
Octyl (C8)
Part No. 188-0310 188-0320 188-0350 188-0356 188-0360
Sample Preparation
AccuBONDII Ethyl (C2) SPE Products

AccuBONDII Ethyl (C2) SPE products are manufactured using a trifunctional silane bonded to irregular silica gel (silica) particles. They are not endcapped, allowing additional polar interactions with surface silanol groups. The short chain ethyl group provides less retention than the longer C8 and C18 chains, where retention is primarily based on non-polar interactions. The stronger interactions of this phase with basic and polar analytes can provide additional extraction selectivity.
Structure of ethyl (C2) silane, covalently bonded to the surface of a silica particle
Description
Ethyl (C2)
Part No. 5185-5811 5185-5812 5185-5813 5185-5814 5185-5815
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AccuBOND" SPE Products AccuBONDII Phenyl SPE Products
AccuBONDII Phenyl SPE products are based on bonded, reversed phase (phenyl), irregular silica gel (silica) particles. This phase exhibits additional polar secondary interactions, which can enhance retention of basic compounds. It exhibits a different selectivity compared with the octadecyl and octyl phases when both aromatic and nonaromatic compounds are being extracted.
Structure of phenyl silane, covalently bonded to the surface of a silica particle
Description
Phenyl
Part No. 188-0510 188-0520 188-0550 188-0556 188-0560
Sample Preparation
AccuBONDII Normal Phase (Non-polar) SPE Products

Normal phase sorbents are polar and used to retain (extract) polar analytes. For normal phase sorbents, solvent strength increases as the solvent becomes more polar.
AccuBONDII Silica SPE Products

AccuBONDII Silica SPE products are based on unbonded, activated irregular silica gel (silica) particles. The primary interaction available is polar (e.g., hydrogen bonding). The silanol groups are ionizable, so it can be used as a weak cation exchanger at intermediate pH values.
Structure of silanol groups on the surface of a silica particle
Description
Silica
Part No. 188-0110 188-0120 188-0150 188-0156 188-0160
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AccuBONDII Normal Phase (Polar) SPE Products

AccuBONDII Cyano (CN) SPE Products
AccuBONDII Cyano (CN) SPE products are based on irregular silica gel (silica) particles. This polar, bonded sorbent can exhibit both polar and non-polar interactions. It can be used as a non-polar sorbent for extraction of both polar and non-polar molecules from aqueous samples, and for extraction of polar molecules from relatively non-polar solvents.
Structure of cyano silane, covalently bonded to the surface of a silica particle
Description
Cyano (CN)
Part No. 188-0610 188-0620 188-0650 188-0656 188-0660
Sample Preparation
AccuBONDII Diol SPE Products

AccuBONDII Diol SPE products are based on irregular silica gel (silica) particles. This polar, bonded sorbent can exhibit both polar and weak non-polar interactions, depending on the cartridge preparation and sample matrix. While, with appropriate cartridge conditioning, it can be used as a non-polar sorbent to extract relatively non-polar molecules from aqueous samples, it is more frequently used to extract polar molecules from relatively non-polar solvents using hydrogen bonding interactions.
Structure of diol silane, covalently bonded to the surface of a silica particle
Description
Diol
Part No. 188-0710 188-0720 188-0750 188-0756 188-0760
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AccuBOND" SPE Products AccuBONDII Amino (NH2) SPE Products
AccuBONDII Amino (NH2) SPE products are based on irregular silica gel (silica) particles. This dual purpose sorbent can act either as a polar phase or weak anion exchanger. When conditioned with a non-polar solvent, e.g., hexane, it can hydrogen bond with any molecule containing OH, NH, or SH functional groups. In an aqueous environment with pH 7.8 or less, it can function as a weak anion exchanger.
Structure of amino (NH2) silane, covalently bonded to the surface of a silica particle
Description
Amino (NH2)
Part No. 188-1010 188-1020 188-1050 188-1056 188-1060
Sample Preparation
1000 mg, 6 mL
AccuBONDII Ion Exchange SPE Products

Ion exchange phases are more dependent on pH, ionic strength, and counter-ion strength than on solvent strength. These phases depend on ionic interactions as the primary retention mechanism.
AccuBONDII Strong Anion Exchange (SAX) SPE Products

AccuBONDII SAX SPE products are based on irregular silica gel (silica) particles. This strong anion exchanger is used to extract compounds capable of carrying a negative charge from both aqueous and non-aqueous solutions. They are ideally suited to extraction of weak acids. Nominal capacity 0.6 meq/g.
Structure of SAX silane, covalently bonded to the surface of a silica particle
Description
Strong Anion Exchange (SAX)
Part No. 188-1610 188-1620 188-1650 188-1656 188-1660
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AccuBOND" SPE Products AccuBONDII Strong Cation Exchange (SCX) SPE Products
AccuBONDII SCX SPE products are based on irregular silica gel (silica) particles. These strong cation exchange sorbents are used to extract positively charged basic compounds. This benzene-sulphonic acid-based sorbent has significant non-polar secondary interactions, with nominal capacity 0.3 meq/g.
Structure of SCX silane, covalently bonded to the surface of a silica particle
Description
Strong Cation Exchange (SCX)
Part No. 188-1510 188-1520 188-1550 188-1556 188-1560
Sample Preparation
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Comparative Herbicide Extractions Using SPE Products

Application Example
Detection of triazine herbicides can be critical to food safety. Analysis of these persistent chemicals in various matrices is simplified by the use of AccuBONDII SPE sample preparation. Examples in soilcontaining a large number of charged species, muscle tissuewith large amounts of relatively non-polar components such as lipids and corn oilwith glycerides and fatty acids that are non-polar compared to the triazines show effective separation of the three prominent triazine species. Refer to the chromatograms to see the chromatographic results. Analysis of Triazines in a Variety of Samples: Preparation with AccuBONDII SPE Cartridges
General Structure of Triazine Herbicides
Solid Phase Extraction Method
Sample Preparation
Results in Chromatogram Soil CARTRIDGE EXTRACTION

Soil
Muscle Tissue ODS (C18) Homogenized in methanol Methanol Diluted with water Water
Corn Oil Diol None Methanol, hexane Diluted with hexane Hexane
SCX Shaken in acetonitrile Acetic acid Diluted with acetic acid Acetic acid, acetonitrile, water, 0.1 M K2HPO4 Acetonitrile/ K2HPO4
PRE-TREAT LOAD WASH
ELUTE
Methanol
Methanol
Key to Chromatograms
Muscle Tissue
Peak 1 2 3 Simazine Atrazine Propazine
R1 CH2CH3 CH2CH3 CH(CH3)2
R2 CH2CH3 CH(CH3)2 CH(CH3)2
Corn Oil
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AccuBONDII Non-Silica SPE Products

Non-silica-based SPE phases have varying degrees of polarity and surface acidity or basicity. They are primarily used to retain polar analytes. For these phases, solvent strength generally increases as the solvent becomes more polar.
AccuBONDII Alumina A (Acidic) SPE Products

AccuBONDII Alumina A sorbents, with 50-200 m particle size, can adsorb molecules by interaction with the aluminum metal center, hydrogen bonding with the surface hydroxyl groups, or ion exchange if the surface carries a charge. Acid washing results in a surface with decreased capacity for basic compounds.
Description
Alumina A (Acidic)
Part No. 188-2110 188-2120 188-2150 188-2156 188-2160
Sample Preparation
AccuBONDII Alumina B (Basic) SPE Products

AccuBONDII Alumina B sorbents, with 50-200 m particle size, can adsorb molecules by interaction with the aluminum metal center, hydrogen bonding with the surface hydroxyl groups, or ion exchange if the surface carries a charge. Washing with a basic solution results in a net negative charge, allowing retention of certain compounds as cations on the surface or by specific interaction with the center.
Description
Alumina B (Basic)
Part No. 188-2210 188-2220 188-2250 188-2256 188-2260
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AccuBOND" SPE Products AccuBONDII Alumina N (Neutral) SPE Products
AccuBONDII Alumina N sorbents, with 50-200 m particle size, can adsorb molecules by interaction with the aluminum metal center, hydrogen bonding with the surface hydroxyl groups, or by ion exchange if the surface carries a charge. The neutralized surface allows interaction with compounds whose heteroatoms are electronegative (e.g., N, O, P, S) or with an electron-rich, highly aromatic structure.
Description
Alumina N (Neutral)
Part No. 188-2310 188-2320 188-2350 188-2356 188-2360
Sample Preparation
AccuBondII Florisil SPE Products

AccuBONDII Florisil is a selective synthetic magnesia-silica adsorbent, specially processed to give consistent results when used for column cleanup and separation of chlorinated pesticide residues prior to identification and measurement of the pesticide by gas, thin layer, or paper chromatography.
Description
Florisil
Part No. 188-2410 188-2420 188-2450 188-2456 188-2460
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SPE
AccuBONDII Cartridge Starter Kits and Bulk SPE Sorbents

AccuBONDII sorbents are available in bulk, along with a selection of reservoirs and frits, for those who wish to pack their own SPE cartridges or to perform flash chromatography.
Description
SPE Starter Kits 10 each in 100 mg, 1 mL configuration
Part No.
AccuBONDII Bulk SPE Sorbents
Kit A (Reversed Phase) C2, C8, C18, CN, and Phenyl Phases Kit B (Normal Phase) Silica, Amino, Diol, and CN Phases Kit C (Ion-Exchange Phase) SCX, SAX, and Amino Phases
009-4003 009-4004 009-4005
Sample Preparation
Description
AccuBOND Bulk SPE Sorbents
II
Unit Bottle of 25 g Bottle of 25 g Bottle of 25 g Bottle of 25 g Bottle of 25 g Bottle of 25 g Bottle of 25 g Bottle of 25 g Bottle of 25 g Bottle of 25 g Bottle of 25 g Bottle of 25 g Bottle of 25 g Bottle of 25 g
Part No. 189-1302 189-0302 5185-5816 189-0502 189-0102 189-0702 189-0602 189-1002 189-1602 189-1502 189-2102 189-2202 189-2302 189-2402
ODS (C18) Octyl (C8) Ethyl (C2) Phenyl Silica Diol Cyano (CN) Amino (NH2) SAX SCX Alumina A Alumina B Alumina N Florisil
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SPE
Evidex" SPE Products Application Example
Opiates in Urine by GC/MS Conditions COLUMN: CARRIER: OVEN: INJECTOR: DETECTOR: DB-5ms-EVDX (Agilent Technologies P/N 128-8522) Helium at 40 cm/sec 65C for 1 min, 65-325C at 20 degrees/min Splitless, 250C MSD, 30C transfer line
EvidexII SPE Products

EvidexII SPE CartridgesOptimum Separation for Drugs-of-Abuse Testing
Effective preparation is an important step in building compelling evidence in drugs-of-abuse cases. The EvidexII SPE cartridgesuitable for all necessary methodssupports the kind of reliable, accurate separation that you need, with characteristics like: Cartridge designed for NIDA-5 drug classes (NIDANational Institute of Drug Abuse is now called SAMSHA Substance Abuse and Mental Health Services Administration) Amphetamine/Methamphetamine PCP Benzoylecgonine Codeine/Morphine THC-COOH (Marijuana) Step-by-step instructions included, for testing consistency Accurate, reproducible results (< 5% RSD)
Sample Preparation
Codeine 30-ng
Morphine 30-ng
Proprietary bonding chemistry (mixed phase) Tested with actual drugs of abuse to ensure lot-to-lot reproducibility, high recovery, and clean extracts with low background Forgiving of minor errors of volumes and concentrations of reagents Specific GC Evidex capillary columns for optimized separations of drug classes
Description 200 mg, 3 mL Unit 50/pk 30/pk Part No. 188-2920 188-2946
Morphine
Codeine
400 mg, 6 mL
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Evidex" SPE Products
Drugs of Abuse Gas Chromatography Columns

DB-EVDX
Specially configured and tested for drugs of abuse confirmation DB-1-EVDX: 100% Dimethylpolysiloxane DB-5ms-EVDX: Equivalent to (5%-Phenyl)- methylpolysiloxane DB-17-EVDX: (50%-Phenyl)- methylpolysiloxane Consistent rentention and peak shape. Test mix includes: caffeine. gluthethimides, lidocaine, phenobarbital, EDDP, Methaqualone, methadone, cocaine, desipramine, carbamazepine Low bleed for GC/MS analysis Bonded and cross-linked Solvent rinsable
Sample Preparation
Helpful Hint: Please contact Technical Support for the test mix specifications.
Phase
ID (mm)
Length (m)
Film (m)
Temp Limits (C)
Part No.
DB-1-EVDX DB-5ms-EVDX DB-17-EVDX
0.20 0.20 0.20
25 25 25
0.33 0.33 0.33
-60 to 325/350 -60 to 325/350 40 to 280/300
128-8122 128-8522 128-8722
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Manifolds and Accessories
Manifolds and Accessories for Convenient, Successful Sample Preparation

Agilent manifolds and accessories complement the quality of ZORBAX and AccuBONDII sorbents. Flexible configurations and availability as complete assemblies or individual components mean that the user has the necessary capability at any stage from method development to high throughput operation.
Cartridge Vacuum Manifold Processing Stations

Vacuum manifold processing stations for individual cartridges are suitable for use from method development to lower volume production. They can be used with individual cartridges or flexible well-plate cartridges. Shown here is a 10-port manifold assembly, (P/N 5185-5754) with all inputs plugged. Your capability is enhanced with features like: Available for either 10 or 20 positions Tank constructed of chemically resistant, vacuum-safe glass
Sample Preparation
Vacuum Manifold Processing Station P/N 5185-5754
Ultra high molecular weight polyethylene lid that ensures excellent solvent resistance Vacuum gauge with coarse and fine control valves for monitoring and adjustment of vacuum levels, plus a safety release valve Choice of height-adjustable racks to accommodate a variety of collection vessels (rack options to fit 10, 12 and 16 mm tube diameters); standard rack for 16 mm tubes Compatible with standard Luer-tipped SPE and filtration columns Individually controlled PTFE stopcocks, permitting flow control at each port position (optional) Clearly marked ports and racks to ensure correct positioning of collection vessels
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Vacuum Manifolds for Individual Cartridges
Description
10-Port Manifolds for Cartridges
Part No. 5185-5754 5185-5755 5185-5756 5185-5757 5185-5760 5185-5761 5185-5762

Stacking Adapter (P/N 5185-5794)
Vacuum manifold assembly, 10 port, w/16 mm rack (adjustable)* Rack, optional, 10 x 10 mm, adjustable height Rack, optional, 10 x 12 mm, adjustable height Rack, optional, 10 x 16 mm, adjustable height Lid, 10 position for 10-port vacuum manifold Lid gasket for 10-port vacuum manifold Glass tank for 10-port vacuum manifold
Luer adapters (P/N 5185-5789) allow use of flexible well-plate cartridges in the 10-or 20-port manifold.
Sample Preparation
* Vacuum manifold can be used with Flexible Plate Cartridges, with addition of Luer adapter for 25/pk (P/N 5185-5789)
Needle Retainer (P/N 5185-5764)
Stopcock Valve (P/N 5185-5758)
Multi-stage preparations are straightforward, with use of the accessories shown.
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Adapters and Replacement Parts

A full selection of adapters allows stacking cartridges for multi-step separation or use of flexible cartridges on 10 or 20-port manifolds. Parts are available for replacement of worn or contaminated parts and subassemblies.
Description
20-Port Manifolds for Cartridges
Unit
Part No. 5185-5765 5185-5766 5185-5767 5185-5768 5185-5771 5185-5772 5185-5773
Vacuum manifold assembly, 20 port, w/16 mm rack (adjustable)* Rack, optional, 20 x 10 mm, adjustable height Rack, optional, 20 x 12 mm, adjustable height
Sample Preparation
The 20-port manifold assembly (P/N 5185-5765) is shown with all three sizes of SPE cartridge, illustrating its adaptability.
Rack, optional, 20 x 16 mm, adjustable height Lid, 20 position for 20-port vacuum manifold Lid gasket for 20-port vacuum manifold Glass tank for 20-port vacuum manifold
Parts and Disposables for Individual Cartridge Manifolds
Stopcock valve for vacuum manifold

Cartridge contents are retained and protected on top by use of the appropriately sized cap (available for 1 mL, 3 mL, and 6 mL cartridges) and on the bottom by a Luer cap (P/N 5185-5795).
10/pk** 10/pk** 10/pk** 10/pk** 100/pk 100/pk 100/pk 10/pk 100/pk
5185-5758 5185-5759 5185-5763 5185-5764 5185-5791 5185-5792 5185-5793 5185-5794 5185-5795
Stainless steel needles for vacuum manifold Port seal plugs for vacuum manifold Needle retainer for 10-port vacuum manifold Caps for 1 mL SPE cartridges Caps for 3 mL SPE cartridges Caps for 6 mL SPE cartridges Adapters, stacking 1, 3, or 6 mL cartridges Luer caps (fit all cartridge sizes)
*Vacuum manifold can be used with Flexible Plate Cartridges, with addition of Luer adapter (P/N 5185-5789) **For a 20 port manifold, order 2 packs of 10.
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Vacuum Manifolds for 96-Well Plates

96-Well Vacuum Manifold Processing Stations
These stations allow configurations that are usable for both method development and routine testing. Flexible 96-well trays are designed for easy configuration with the selection of cartridges you need for method development. Fill a row, column, or other arrangement, and cap the rest. Fixed 96-well trays support higher volume, automated production. All wells are packed with the same sorbent and mass as the comparable flexible well cartridge, ensuring uniform, predictable separations. Vacuum manifolds for 96-well trays are designed to allow both convenient method development and high throughput operation. The base accommodates lids that hold either a userdefined selection of flexible cartridges, or fixed format 96-well plates with each well loaded with the same sorbent. The processing station can accommodate either fixed position plates or flexible format plates with quality features like: Constructed with polypropylene base and polyethylene lid (choose flexible and/or fixed format lid) On-off switch, vacuum gauge, and fine vacuum control valve for full control Disposable reservoir tray to collect excess sample and wash solvents Allows spacer inserts to be placed into the base so that collection plates of differing heights can be processed (both deep well and standard microtiter plates); ensures maximum penetration of the SPE plate into the collection plate and reduces possible well-to-well contamination Solvent-resistant manifold lid gasket Small footprint
1 9 8 7 3 6 1 4 5 2
The system is shown here with a fixed format tray, comprising: 1. Basecomplete assembly (P/N 5185-5797) Replacement parts: 2. Vacuum gauge (P/N 5185-5786) 3. On/off valve (P/N 5185-5785) 4. Needle valve (P/N 5185-5783) 5. Vacuum outlet (P/N 5185-5784) The setup shown includes: 6. Lid for fixed well manifold (P/N 5185-5798) 7. Fixed 96-well plate
Sample Preparation
Note: Must order base (P/N 5185-5797) and one lid, either fixed plate lid (P/N 5185-5798) or flexible plate lid (P/N 5185-5799). See page 137 for prices.
A 96-well manifold system is shown here equipped for flexible format plates, comprising: 1. Basecomplete assembly (P/N 5185-5797) 8. Lid for flexible format manifold (P/N 5185-5799) 9. Collection plate
Vacuum Manifolds for 96-Well Fixed or Flexible Plates

Description Base for 96-well vacuum manifold (includes vacuum gauge, needle valve, etc.). Select the appropriate lid, etc., for fixed (5185-5798) or flexible format (5185-5799). See page 137. Part No. 5185-5797
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Parts and Disposables for 96-Well Plates
Description
1 2 3
Unit 32/pk
Part No. 5042-1387 5065-4402
96-well deep well collection plates, 1 mL, polypropylene 96-well collection plates with glass inserts, caps, and septa, pre-assembled, 0.35 mL 96-well collection plates, 0.5 mL, polypropylene 96-well collection plates, 0.5 mL, polypropylene Base O ring for 96-well plate manifolds Base plate/flexible format 96-well cartridges Closing mats Collection plate spacer for Agilent 1.0 mL deep well, 12 mm
10/pk 120/pk
5042-1386 5042-1385 5185-5779 5185-5787
Collection plates for 96-well manifolds: 1. Pre-assembled system with glass inserts, caps, and septa (P/N 5065-4402) 2. Deep well collection plate (P/N 5042-1387) 3. 0.5 mL collection plate (P/N 5042-1386)
50/pk
5042-1389 5185-5775 5185-5781
Sample Preparation
Collection plate spacer for microtiter plate and Agilent 0.5 mL shallow well plate, 29 mm
Base O-ring (P/N 5185-5779)
Collection plate, showing 96-position closing mat (P/N 5042-1389)
Collection plate spacer, in sizes to match the collection plate used (P/N 5185-5775)
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Vacuum Manifolds for Individual Cartridges
Description Collection plate spacer for most industrystandard deep well plates, 2 mm Disposable reservoir tray, 96-well manifold Lid for 96-well fixed well vacuum manifold Lid for 96-well flexible well vacuum manifold Lid gasket for 96-well plate manifold Luer adapters for 96-well flexible cartridge Needle valve for 96-well manifold On/off valve for 96-well manifold Strip of 8 base plate Luer plugs Vacuum gauge for 96-well manifold Vacuum outlet (Ni plated) 96-well manifold Well insertion/removal tool for flexible plate cartridge 50/pk 25/pk 25/pk Unit Part No. 5185-5780 5185-5782 5185-5798 5185-5799 5185-5778 5185-5789 5185-5783 5185-5785 5185-5788 5185-5786 5185-5784 5185-5790
Lid for flexible format manifold (P/N 5185-5799) Disposable reservoir tray (P/N 5185-5782)
Sample Preparation
SPE Accessories for Discontinued J&W Manifold 600-4000

Description Vacuum gauge 1/8 in. NPT, 0-30 in. Hg Port sealing plugs Lid gasket Luer fittings, female Teflon tubing guides 10/pk 12/pk 10/pk Unit Part No. 715-0006 5185-5763 701-4000 5185-5764 701-4005
Luer adapters for flexible cartridges for use in standard 10 and 20-port vacuum manifold (P/N 5185-5789)
Strip of base plate Luer plugs for capping unused base plate holes in flexible format configuration (P/N 5185-5788)
Well insertion/removal tool for flexible plate cartridges (P/N 5185-5790)
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AccuBOND" Reservoirs and Frits
AccuBONDII Empty Reservoirs and Frits

Empty reservoirs and separate or integrated frits are available if you would like to prepare your own solid phase extraction cartridges. By purchasing bulk SPE sorbents, page 129, you can pack different masses of sorbent, mixed phases or any combination that you desire. The larger volume reservoirs can be used for low pressure or flash chromatography.
Description Empty reservoirs, Luer fitting on bottom, 3 mL Empty reservoirs, Luer fitting on bottom, 6 mL Empty reservoirs, Luer fitting on bottom, 25 mL Empty reservoirs, Luer fitting on bottom, 70 mL Frits for reservoirs 3 mL, polythylene* Frits for reservoirs 6 mL, polythylene* Reservoirs with 2 x 20 m frits, 3 mL** Reservoirs with 2 x 20 m frits, 6 mL**
Unit 50/pk 50/pk 50/pk 50/pk 100/pk 100/pk 50/pk 50/pk
Part No. 700-4005 700-4006 700-4007 700-4008 700-4030 700-4031 700-4015 700-4016
*Use two frits in each tube. **Used with high-suspended-solids samples.
Sample Preparation
138
CombiZorb Scavengers CombiZorb Scavengers A Purification Tool for Combinatorial Chemistry

Less Swelling Broad Solvent Compatibility More Capacity per Unit Volume Drug discovery frequently requires compounds to be of high purity in order to properly evaluate their bioactivity and to produce pure combinatorial compound libraries. CombiZorb Scavengers allow the rapid removal of undesired reaction by-products and excess reactants. CombiZorb Scavengers have been developed to solve some of the recurrent problems associated with commercial products such as the polystyrene-divinylbenzene (PS-DVB) copolymersswelling, lack of solvent compatibility, static charge, and handling difficulties. CombiZorb Scavengers come in two varieties: CombiZorb S Silica-Based Scavengers and CombiZorb MP Macroporous Polymer-Based Scavengers. They are sold in bulk in 10 g and 100 g bottles (one kilogram bottles on request as a special quote). CombiZorb Scavengers can be packed into flow-through cartridges, 96-well plates or used in solution.
CombiZorb S Scavengers
CombiZorb S Silica-based Scavengers are synthesized from Type B high purity spherical, rigid silica gel particles with low trace metal content. Scavenger phases are covalently bonded to the base silica using organosilane-bonding chemistry. The CombiZorb S products: Show no swellingunlike conventional functionalized gel-type polystyrene/divinylbenzene resins that can swell up to 500% of their dry volume. Free-flowing, easy to handle when packing columns, 96-well plates, and when transferring the sorbent; no static charge. Are based on high purity (99.995%) porous silica microspheres with low metal content; no interfering reactions or catalysis with metals or contamination from sorbent.
Sample Preparation
CombiZorb MP Scavengers
CombiZorb MP Polymeric Scavengers were created to overcome the problems with lightly crosslinked polymer products. The CombiZorb MP products: Have significantly less swelling (maximum 50%) than standard PS/DVB resins (>500%); they show higher, more consistent capacity/volume and do not need to pre-swell. Show broad solvent compatibility and can be used with polar or non-polar solvents between reaction and scavenging steps and there is no need to switch solvents. Have high purity with no extractables to foul reaction products. Helpful Hint: If you are unsure as to the CombiZorb Scavenger product that may fit your needs, a convenient Variety Pack (P/N 550550-901) is available. This pack contains six of the most popular CombiZorb phases.
139
CombiZorb Scavengers
CombiZorb Physical Data
CombiZorb Scavengers Structure Characteristics Loading Capacity Applications
CombiZorb S Scavengers
S-Triamine
Si
H N
)2 NH2
Spherical, ultra-pure silica Density: 1.3 g/mL Particle Size: 2080 m No swelling Spherical, ultra-pure silica Density: 1.3 g/mL Particle Size: 2080 m No swelling Spherical, ultra-pure silica Density: 1.3 g/mL Particle Size: 2080 m No swelling Spherical, ultra-pure silica Density: 1.3 g/mL Particle Size: 2080 m No swelling
1.21.6 meq/g
Scavenging acids, acid chlorides, acid anhydrides, chloroformates, isocyantes, aldehydes and many other electrophiles Scavenging acids, acid chlorides, acid anhydrides, chloroformates, isocyantes, aldehydes and many other electrophiles Scavenging amines and other basic compounds
S-Monoamine
Si
NH2
0.60.9 mmol/g
S-Sulfonic acid
Si
SO3H
0.40.6 mmol/g
Sample Preparation
S-Tertiary amine
Si
NMe2
0.60.9 mmol/g
Scavenging acids generated from condensation reactions and may catalyze acylation reactions
CombiZorb MP* Scavengers
MP-Aldehyde
MP
Spherical, macroporous polystyrene/DVB particle Density: 0.6 g/mL CHO Particle Size: 30100 m Swelling: 2030% in THF or CH2Cl2 Spherical, macroporous polystyrene/DVB particle Density: 0.6 g/mL Particle Size: 30100 m Swelling: 2030% in THF or CH2Cl2 Spherical, macroporous polystyrene/DVB particle Density: 0.6 g/mL Particle Size: 30100 m Swelling: 3050% in THF or CH2Cl2
1.21.8 mmol/g
Scavenging hydrazines, hydroxylamines, primary amines, organometallic reagents (organolithium and Grignard reagents), and other nucleophiles Scavenging hydrazines, hydroxylamines, primary amines, organometallic reagents (organolithium and Grignard reagents), and other nucleophiles Scavenging aldehydes and ketones and used as solid phase reagents in some catch and release applications
0.91.3 mmol/g
MP-Isocyanate
MP
NCO
1.52.5 mmol/g
MP-Sulfonyl hydrazide MP
SO2NHNH2
* MP-macroporous polystyrene/divinylbenzene
140
CombiZorb Scavengers
CombiZorb Physical Data (continued)
CombiZorb Scavengers Structure Characteristics Loading Capacity Applications
CombiZorb MP Scavengers
MP-Sulfonyl chloride
MP
SO2Cl
Spherical, macroporous polystyrene/DVB particle Density: 0.6 g/mL Particle Size: 30 100 m Swelling: 30 50% in THF or CH2Cl2 Spherical, macroporous polystyrene/DVB particle Density: 0.6 g/mL Particle Size: 30 100 m Swelling: 30 50% in THF or CH2Cl2
1.6 2.0 mmol/g
Scavenging alcohols, phenols, and amines, more widely used as solid phase reagents in some catch and release applications
1.7 2.5 mmol/g
MP-Piperidinomethyl
MP
Use as catalyst for acylation reactions in place of tertiary amine and for scavenging acids generated from condensation reactions
Note: To get the complete CombiZorb Scavenger story with practical applications examples, please go to the Agilent website www.agilent.com/chem and print a copy of Publication #5968-7313E.
Sample Preparation
CombiZorb Scavenger S-Triamine* S-Monoamine* S-Sulfonic acid* S-Tertiary amine* MP-Aldehyde* MP-Isocyanate* MP-Sulfonyl hydrazide MP-Sulfonyl chloride MP-Piperidinomethyl Variety pack
Part No. 550145-138 550245-238 550145-118 550245-218 550145-124 550245-224 550145-148 550245-248 510100-150 510200-250 510100-160 510200-260 510100-134 510200-234 510100-114 510200-214 510100-170 510200-270 550550-901
Size 10 g 100 g 10 g 100 g 10 g 100 g 10 g 100 g 10 g 100 g 10 g 100 g 10 g 100 g 10 g 100 g 10 g 100 g (6) 5 g Bottles
*Included in Variety Pack
CombiZorb Scavenger
141
Sample Filtration
Syringe Filters
Sample Filtration
Syringe Filters
Membrane syringe filters are recommended to clarify any samples that need further analysis or use where particulate matter in the sample solution may cause a problem. Most often they are used for small volume HPLC samples. The membrane filters are contained in an inert polymeric housing. No glue or binders are used in their construction to ensure that no extractables are present. The housing is designed to ensure that the sample is spread over the whole surface of the membrane so that maximum membrane capacity is used. Syringe filters are ready-to-use and are quite convenient. You merely attach the Luer-tipped syringe filled with the sample solution to the housing and push the sample through the pre-cleaned filters. Why Filter Your Samples for HPLC? Protects your column, particularly the newer sub-five micron microparticulate columns, capillary columns and column inlet frits, against plugging (blockage) from sample particulate matter. Extends column lifetime.
Sample Preparation
Protects your injection valve components from possible damage, scratching, and increased wear by sample particulate matter. Minimizes instrument downtime. Why Purchase Agilent Syringe Filters? We offer the most popular sizes, porosities, and membrane types at attractive pricing Both Luer and the lowest volume mini-tips available All syringe filters are pre-sterilized by gamma irradiation Low extractables for high sensitivity work High protein recovery for biological samples Regenerated cellulose filters are batch-tested under HPLC conditions New Econofilters are attractively priced in larger quantity packages How to Select the Right Membrane Syringe Filter? 1) Choose the size of filter based on the volume of sample that must be filtered. 2) Choose the porosity of the filter based on the size of potential particulates that may be present in your sample. Remember, the finer the porosity the more pressure it will take to pass sample through the filter. If you have a great deal of particulate matter, you may want to use a glass fiber filter, place a glass fiber pre-filter in front of your membrane filter to prevent rapid plugging or use a 2-in-1 filter that has a built-in prefilter in a single housing. 3) Choose the membrane type based on the solvent that you want to filter.
142
Sample Filtration
Syringe Filters Filter Types
All filter inlets are female Luer-compatible, have inert polypropylene or polycarbonate housings and come in four diameters: 30 mm filters are designed for larger sample volumes or for solvent filtration. The wide cross sectional area (5.1 cm2) offers an increased filtration speed over the smaller diameter filters. The holdup volume is less than 50 L. 25 mm Econofilters are designed for high throughput laboratories that require an economical filter packaged in larger quantities at a substantial cost savings. The filters have a moderately wide cross sectional area (4.2 cm2) with a holdup volume of less than 50 L. 13 mm filters are ideal for most applications. They offer the best compromise between holdup volume and sample volume and are our most popular size. Sample volumes are typically in the 1-10 mL range and the holdup volume is less than 10 L. 3 mm filters are ideal for small sample (< one mL), where a minimum loss of sample is desirable. The membranes and housings are compatible with both organic and aqueous samples. The holdup volume is less than 7 L.
Sample Preparation
Two porosities are available: 0.45 micron pores and 0.20 micron pores. Use the 0.45 micron version to remove particles that are detrimental to most columns; the 0.20 micron filters are used to remove the smallest particles. Pre-filters have a 100% borosilicate glass fiber membrane that is chemically inert and resistant to most solvents. The high surface area of the rigid fiber structure provides outstanding particle retention capacity while maintaining low flow resistance. The GF53 will retain coarse particles down to approximately 3 m in diameter and the GF92 down to approximately 2 m in diameter. They can be used standalone or in series with a membrane filter. 2-in-1 filters are a two-layered filter in a single housing with a built-in glass fiber pre-filter on the top layer and a membrane filter on the bottom layer. The coarse pre-filter removes the larger particulates that would plug the membrane filter. This type of filter is recommended for difficult-to-filter samples. Because the pre-filter retains coarse particles, it requires less force to push liquid sample through the filter, thereby providing higher throughput. The combination costs less than two individual filters and saves time, sample and money.
Glass Fiber Prefilter Membrane Filter
Membrane Types
Agilent Technologies provides the most popular type of membranes to cover a very wide range of applications. The main selection criteria are solvent-compatibility and porosity. To assist you in making sure that the former criterion is met, the chemical resistance table lists the most popular solvents used in HPLC along with the compatible membrane types. As you can see, the regenerated cellulose and PTFE membranes have the widest range of solvent compatibility and are the preferred membranes for HPLC use. Our most popular membrane type, the regenerated cellulose membrane is priced slightly lower than the PTFE.
2-in-1 Filter
143
Sample Filtration
Syringe Filters
PTFE membranes are compatible with almost all solvents, acids and bases. Nylon 66 membranes are compatible with most solvents, organic and aqueous; but use with strong acids, methylene chloride and DMF is not recommended. Cellulose nitrate is primarily used for pre-filters and is compatible with many but not all aqueous or non-aqueous solvents (see Chemical Resistance Table). Cellulose acetate membranes are not compatible with organic solvents. They are well suited for aqueous solutions and are specially recommended for proteins and protein-related samples. Regenerated cellulose membranes are recommended for general HPLC sample preparation as well as filtration of aqueous biological samples and organic solvents. The regenerated cellulose filter is close to a universal filter for HPLC. In addition to its wide range of solvent compatibility, low protein binding, and attractive pricing, it offers extremely low extractables. Extractables with uv-absorbance can be particularly troublesome to liquid chromatographers. Thus, all regenerated cellulose filters are batch-certified for water, acetonitrile and methanol extractables as determined under stringent HPLC test conditions (wavelengths of 214 and 254 nm) on an Agilent Technologies Series 1100 HPLC System. A certificate of analysis (C of A) is available for each batch of regenerated cellulose filters. In addition, we have changed the packaging and provide the regenerated cellulose in a reusable, easy-opening plastic box.
Sample Preparation
Chemical Resistance Table for Membrane Filters*

Substances Cellulose nitrate 1-Hexanol 1.4-Dioxan Acetic acid, 10% & 25% Acetone Acetonitrile Aliphatic hydrocarbons Ammonia, 1 M Aromatic hydrocarbons Benzene Boric acid Carbon tetrachloride Carboxylic acid Chloroacetic acid Chloroform Cyclohexane Cyclohexanol + + + + + + + + + + o + + o + + + + + o + o + Membrane filters Cellulose acetate Regenerated cellulose + + + + + + + + + + + + o + + + Nylon 66 + N/A + + + + N/A + o + + + PTFE + o + + + + + + + + + + o + +
*Code for Table: + = Resistance, o = Limited resistance, = Not resistant, N/A = Not available
144
Sample Filtration
Syringe Filters
Chemical Resistance Table for Membrane Filters (continued)*
Substances Cellulose nitrate Diethylether Dimethyl formamide Dimethyl sulfoxide Ethanol=<98% Ethyl acetate Ethylene chloride Ethylene glycol Formic acid, 25% Hexane Hydrochloric acid, 25% i-Propanol Methanol Nitric acid, 25% Pentane Phosphoric acid, 25% Phosphoric acid, 45% Potassium hydroxide, 1 M Salt solutions, aqueous Sodium hydroxide, 1 M Tetrachloroethane Tetrahydrofuran Toluene Trichloroacetic acid, 10% Trichloroethane Trichloroethylene Xylene o o o + + + + o + + o + + + + + + + o + o + o + + + + o + + o + o o + o o + Membrane filters Cellulose acetate Regenerated cellulose + o o + + + + + + + + + + + + o o + + + + + + + Nylon 66 + + N/A + + + + + + o + o o + o + + o o o + PTFE o + + + + + + + + + + + + + + + + + + + o + + + + +
Sample Preparation
*Code for Table: + = Resistance, o = Limited resistance, = Not resistant, N/A = Not available
145
Sample Filtration
Syringe Filters Recovery
Sample recovery is an important performance criterion for membrane filters. Nobody wants to spend time collecting and preparing their precious sample only to find that in the filtration step, the sample is irreversibly sorbed, affecting the analytical results or reducing the amount of sample analyte for future steps in the process.
Sample Preparation
Recovery of Polynuclear Aromatic Hydrocarbons
Low Protein Adsorption of Cellulose Acetate and Regenerated Cellulose Membranes
Pre-Filters (Stand alone) (100/pk)

Description Part No.
Pre-filter glass fiber GF53 30 mm Pre-filter glass fiber GF91 30 mm
5042-1393 5042-1370
Membrane Filters (100/pk)

Filter PTFE 30/20 with Luer tip Filter PTFE 30/45 with Luer tip Filter PTFE 13/45 with Luer tip Filter PTFE 13-45 with mini tip Filter cellulose acetate 30/45 with Luer tip Filter nylon 3/45 with Luer tip Filter nylon 3/20 with Luer tip
3150-0753 3150-0754 5185-5836 5185-5837 5061-3363 5061-3367 5061-3368
146
Sample Filtration
Syringe Filters
Regenerated Cellulose Membrane Filters (100/pk)
Filter regenerated cellulose 30/20 with Luer tip Filter regenerated cellulose 30/45 with Luer tip Filter regenerated cellulose 13/20 with Luer tip Filter regenerated cellulose 30/45 with Luer tip Filter regenerated cellulose 13/20 with Luer tip Filter regenerated cellulose 13/45 with Luer tip
5061-3354 5061-3364 5064-8222 5064-8221 5061-3366 5061-3365
2-in-1 Filters
2-in-1 Filter cellulose nitrate glass fiber 2-in-1 Filter regenerated cellulose glass fiber
5042-1391 5042-1392
Sample Preparation
Membrane Econofilters (200/pk)

Econofilter regen cellulose 25/20 Econofilter regen cellulose 25/45 Econofilter nylon 25/20 Econofilter nylon 25/45 Econofilter PTFE 25/20 Econofilter PTFE 25/45
5185-5830 5185-5831 5185-5832 5185-5833 5185-5834 5185-5835
Syringes for Use with Sample Filters (10/pk)

Disposable syringes 20 mL
5062-8534
147
GC and GC/MS
Table of Contents
GC and GC/MS Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .149 GC Supplies Septa, Premium and General Purpose . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .149153 Merlin Microseal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .154 Inlet Liners . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .155159 Ferrules . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .160163 Column Connectors/Splitters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .164166 Valves and Loops . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .167168 GC Instrument Parts and Supplies Purge and Trap Supplies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .169172 Automatic Liquid Sampler Supplies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .173 Headspace Supplies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .174175 Split/Splitless Inlet Supplies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .175176 6890 PTV Supplies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .177 6890 Cool On-Column Inlet Supplies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .178179 6890/6850 Packed Port Inlet Supplies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .180 6890/6850 Detector Supplies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .181 6890/6850 FID Supplies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .182183 6890 NPD Supplies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .184185 6890/6850 TCD Supplies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .185186 6890 ECD Supplies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .187 6890 FPD Supplies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .187 5890 Supplies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .188192 5890 Detector Supplies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .192-198 GC/MS Parts and Supplies MSD Maintenance Schedule . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .199203 MSD Ion Source . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .204207 MSD Filaments . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .207 MSD Vacuum System and Pumps . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .208209 MSD Electron Multipliers and Replacement Horn . . . . . . . . . . . . . . . . . . . . . . . . . . .210 MSD NoVents . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .211212 8270 Semi-Volatiles Application Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .212 MS Engine Supplies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .213215 MS Test and Performance Sample . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .216 GC and GC/MS Columns . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .217323 GC/MS Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .324340 GC/MS Applications Retention Data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .341347 Environmental . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .347380 Food and Flavors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .381393 Industrial Chemicals . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .394414 Life Sciences . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .415424 Petroleum . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .425441
GC and GC/MS
148
GC Supplies
Septa
Ensuring Peak Performance in Your Application

Agilent has expanded its offering of GC columns and supplies by adding not just new products, but a powerful brandJ & W Scientific. The selection listed here now presents a range that is virtually unmatched in the industry. That means you can find precisely what you need from a single supplier, whatever your application. What's more, you'll know that each product has been developed and tested to optimize your Agilent system. And the performance of every column is certified to match our published quality control specifications for reliable, repeatable results. In this section, you'll see not only listings of parts and supplies, you'll see highly useful product selection guides, compatibility charts, and chromatograms to help you make the right choices for your specific applications. To make it still more useful, we have integrated a troubleshooting and maintenance guide into this section. Now everything you need to ensure peak performance is right at your fingertips.
Septa
One of the key components of sample introduction is the inlet septum. All columns must have carrier gas head pressure to establish flow through the column. Septa maintain the leak-free seal and exclude air from the inlet. They come in many different sizes and are made from many different types of material specific to inlet type and analysis needs. Septa are usually available according to their recommended upper temperature limits. Lower temperature septa are usually softer, seal better, and can withstand more punctures (injections) than their high-temperature counterparts. If used above their recommended temperatures, however, they can leak or decompose. This causes sample losses, lower column flow, decreased column life and ghosting.
GC and GC/MS
What is the Function The septum isolates the sample flow path from the outside world. It must provide a barrier that is readily penetrated by the injector needle while maintaining internal pressure without contaminating the analysis. Septa are generally made of special high temperature, low-bleed silicone rubber formulations.
Why Replace Septa should be replaced regularly to avoid: leaks decomposition sample loss reduced column or split vent flow ghost peaks column degradation
How to Minimize Problems Avoid problems by: using with the recommended temperature range changing regularly installing the retainer nut hand tight using septum purge when available using autoinjectors using sharp syringe needles
149
GC Supplies
Septa Septa Troubleshooting
Symptom Extra Peaks/Humps Possible Causes Septum bleed. Remedy Turn off injector heater. If extra peaks disappear, use septum specified for higher temperature or analyze at lower inlet temperature.
Baseline Change After Large Peak
Large leak at septum Replace septum and use during injection and smaller diameter needles. for a short time thereafter (common with large diameter needles).
GC and GC/MS
Retention Times Prolonged
Carrier gas leaks at Check for leaks. Replace septum or column septum or tighten connection. connections if necessary.
Summary of Premium Inlet Septum Characteristics

Septum Type BTO (Bleed and Temperature Optimized) Long Life Advanced Green Bleed

Lifetime

Temperature Limits to 400C
(optimized for high temperature)

to 350C to 350C
= best = very good = good
150
GC Supplies
Premium Septa Premium Septa
Premium CenterGuide Septa for Improved Performance
Our premium septa have a recess on the injection side, to guide the syringe needle to the same point with every injection. Center point guides the needle for easy penetration and less coring Reduces needle bending Precision molding assures accurate fit in the inlet Each batch tested on an Agilent 6890 GC-FID for bleed
Bleed and Temperature Optimized Septa (BTO)

Extended temperature range, low-bleed Maximum injection port temperature 400C Virtually eliminates injection-port sticking Pre-conditioned; packaged in glass to prevent contamination Ideal for use with low-bleed, Mass Spec capillary columns
P/N 5183-4757
Bleed and Temperature Optimized Septa

Description 11 mm septa for 4890, 5890, 6850 and 6890 GCs 5 mm septa through-hole for on-column Unit 50/pk 50/pk Part No. 5183-4757 5183-4758
GC and GC/MS
Long-Life Septa
Pre-pierced for extended life and reduced coring The preferred septum for autosamplers Ideal for overnight runs Up to 400 injections per septum Maximum injection port temperature 350C Soft, 45 Durometer, easy on autosampler needles
P/N 5183-4761
Long-Life Septa
151
GC Supplies
Premium/General Purpose Septa
Advanced Green Septa
True long-life, high temperature green septum More injections per septum Reduced injection port sticking Maximum injection port temperature 350C Packaged in glass vials for high purity Economical alternative to competitors Green septa
P/N 5183-4759
Agilent Advanced Green Septa

Bleed Test - Comparison of GC Septa

GC conditions for all chromatograms Instrument: Agilent 6890
GC and GC/MS
Column: P/N: Carrier: Oven:
Injector: Detector:
DB-5 15 m x 0.53 mm id, 1.5 pm film 125-5012 Helium at 4 mL/min constant flow 50C for 15 min 50-250 at 20C/min 250C for 15 min Splitless, 250C 10 minute purge activation time FID, 280C
General Purpose Septa

Agilents General Purpose Septa are made from an enhanced injection-molded silicone rubber material. The septa material, dark red or gray in color, is specified to withstand over 200 automatic injections at an injection port temperature of 350C. You can have confidence in your chromatographic results knowing that each lot of septa is placed through a demanding QC test to ensure that only the highest quality product is delivered to your laboratory.
152
GC Supplies
General Purpose Septa
General Purpose Septa are a cost effective choice which provide
Low bleed for reduced instrument maintenance downtime and increased laboratory productivity Less frequent replacement for long life time and the ability to withstand more than 200 injections at maximum injection port temperatures Easy penetration and the durability to remain resistant to coring and leaking from multiple injections Packaged in airtight jars to minimize the risk of contamination and the need for septa reconditioning
General Purpose
Description Low-Bleed Gray Septa 11 mm for 5880, 5890, 6850 and 6890 GCs 11 mm for 5880, 5890, 6850 and 6890 GCs 9.5 mm (3/8 in.) for 5700 series and 5830/40 GCs 9.5 mm (3/8 in.) for 5700 series and 5830/40 GCs Low-Bleed Red Septa 11 mm solid for 5880, 5890, 6850 and 6890 GCs 11 mm with partial through-hole for 5880, 5890, 6850 and 6890 GCs 5 mm through-hole for on-column inlets, automatic or manual injections 5 mm solid for high column backpressure, on-column inlets 9.5 mm (3/8 in.) for 5700 series and 5830/40 GCs Blue Septa 11 mm for 5880, 5890, 6850 and 6890 series GCs White Septa 9.5 mm (3/8 in.) Teflon backed for 5700 series and 5830/40 GCs 9.5 mm (3/8 in.) Teflon backed for 5700 series and 5830/40 GCs 12/pk 100/pk 5080-8745 5080-8707 5/pk 9301-1083 25/pk 25/pk 25/pk 25/pk 24/pk 5181-1263 5181-3383 24/pk 144/pk 24/pk 144/pk 5080-8896 5080-8894 5080-8728 5080-8726 Unit Part No.
GC and GC/MS
5181-1260 5181-1261 5181-1283
153
GC Supplies
Merlin Microseal The Merlin Microseal
Low bleed, longer life alternative to standard septa for split/splitless injection Has a lifetime of more than 2000 injections, depending on samples and operating conditions Greatly reduced instrument downtime for septa changes and injection port liner changes due to septa particulates Two distinct sealing mechanisms: Double O-ring type seal around the syringe needle Spring assisted duckbill to seal the injection port
Merlin Microseal
Description High Pressure High pressure Merlin Microseal starter kit (microseal septa and nut) Microseal high pressure septum* Microseal high pressure nut High sample volume septum kit contains: Merlin high pressure Microseal, six 23-gauge syringes, 500 vials and caps Standard Pressure Microseal septum, SS, rubber (30 psi) Microseal PTFE nut liners Merlin Microseal Kit original low pressure system includes nut and septum Merlin Microseal Kit original low pressure system includes nut and 2 septum 2/pk 5182-3442 5182-3444 5182-3445 5181-8839 Unit Part No.
GC and GC/MS
5181-8815 5182-0853 5181-8816 5181-8833

*Not interchangeable with older version.
154
GC Supplies
Inlet Liners
Inlet Liners
Agilent offers a complete selection of liners for your Agilent GCs inlet. These liners are designed and engineered to meet strict Agilent specifications for dimensional accuracy and inertness towards demanding compounds. For example, the biggest difference between Agilent splitless liners versus competitor designs is the OD of our splitless liners are ground and polished to exacting dimensional tolerances. This ensures a perfect fit into our inlets and optimum splitless injection performance. Choosing the proper liner for a specific application can be a difficult and challenging task. The three liner characteristics that must be considered for each application are: Liner volume Liner treatments or deactivation Any liner design features that might affect carrier gas flow through the inlet or sample vaporization What is the Function Liners are the centerpiece of the inlet system in which the sample is vaporized and brought into the gas phase. Why Replace These problems will occur if the liner is not changed on a regular basis or if the correct liner is not used: peak shape degradation solute discrimination poor reproducibility sample decomposition ghost peaks How to Minimize Problems Change liners on a regular basis determined by: previous use pattern sample cleanliness chromatographic abnormalities such as: peak shape changes peak discrimination poor reproducibility sample pyrolysis
GC and GC/MS
Liner Troubleshooting
Symptom Tailing Peaks Possible Causes Sample components adsorbed by column, inlet liner or contaminated gold inlet seal. Remedy Use new, deactivated liner or clean old liner and replace glass wool.
Needle hitting and breaking packing in inlet liner. Partially remove packing from liner or use without packing. Column end poorly cut (sample absorption). Normal Problem Remove column. Make a clean, square cut using a reliable capillary fused silica cutting tool (such as a ceramic wafer or the Agilent Column Cutter), then reinstall column. Make sure total flow in inlet is above 40 mL/min. Remove inlet liner and check cleanliness. Use new, deactivated liner or replace glass wool and packing.
Broken or chipped inlet liner. Leading Peaks Sample decomposing.
Normal
Problem
155
GC Supplies
Inlet Liners
Symptom Baseline Rise Before or After Peak Possible Causes Remedy
Sample decomposing. Remove inlet liner and check cleanliness. Use new, deactivated liner or replace glass wool and packing.
Normal
Problem
Problem Column and inlet liner misaligned. Check installation of column end and inlet liner, adjust if necessary.
Baseline Change After Large Peak
Normal Unresolved Peaks
Problem
Problem Column or inlet liner Use a guard column to contaminated or prolong column life. column deteriorating. Remove inlet liner and check cleanliness. Use new, deactivated liner or place or replace glass wool and packing. Trim the front end of the column a minimum of 6 inches.
GC and GC/MS
Normal
Problem
Problem
156
GC Supplies
Inlet Liners Inlet Liners
Liner Volume Description
Split/Splitless Inlet Liners
Unit 5/pk 25/pk
(L) 870
1/pk 5183-4711
Liner, general purpose split/splitless, glass wool, taper, deactivated

Splitless Inlet Liners
5183-4712 5183-4713
Liner, splitless, single-taper, no glass wool, deactivated Liner, splitless, single-taper, glass wool, deactivated Liner, splitless, double-taper, no glass wool, deactivated
Split Inlet Liners
900
5181-3316 5183-4695 5183-4696
900
5062-3587 5183-4693 5183-4694
800
5181-3315 5183-4705 5183-4706
Liner, split, low press. drop, glass wool, taper, deactivated
870
5183-4647 5183-4701
GC and GC/MS
5183-4702 Split Inlet Liner 990 Liner, split, glass wool, non-deactivated Split Inlet Liners for Manual Injection Liner, split, with cup, no glass wool Liner, split, with cup, glass wool, and packing (not recommended for use with electronic pressure control [EPC]), for manual injection
Direct Inlet Liners
19251-60540 5183-4691 5183-4692 18740-80190 5183-4699 5183-4700
800
800
18740-60840 5183-4697 5183-4698
Liner, direct, 2 mm id, deactivated
250
5181-8818 5183-4703 5183-4704
Liner, direct, 2 mm id, non-deactivated, quartz Liner, direct, 1.5 mm id, nondeactivated (use for gas samples, headspace, or purge and trap applications) Liner, straight, splitless 4.0 mm id
250
18740-80220 5183-4707 5183-4708
140
18740-80200 5183-4709 5183-4710
990
210-3003 210-3003-5
157
GC Supplies
Inlet Liners
Direct Connect Liners
Press fit connection to the column No sample exposure to the inlet Included in 8270 EPA application kit Optimized hole size and placement
Description Single taper direct connect liner, splitless 4 mm id, deactivated Dual taper direct connect liner, splitless 4 mm id, deactivated Part No. G1544-80730 G1544-80700
Focus Liners
Improved reproducibility Precise amount of glass wool in the ideal position traps non-volatile sample residue Glass wool wipes any residual sample from the needle
Description Unit Part No.
Focus liner, tapered, 4.0 mm, glass wool, 6.3 mm x 78.5 mm Focus liner, tapered, 4.0 mm, glass wool, 6.3 mm x 78.5 mm
5/pk 5/pk
210-4004-5 210-4022-5
Megaliners
GC and GC/MS
Directly inject samples into 0.45 or 0.53 mm ID columns Column seals to the base of the liner reducing solvent tailing Virtually all of the sample transfers to the column
Megaliner 22 gauge, 4.0 mm, no glass wool, 6.3 x 78.5 mm Megaliner 26 gauge (T-top), 4.0 mm, no glass wool, 6.3 x 78.5 mm
210-2063H22 210-2063H26
Liner O-Rings
Liners are sealed in the inlet with O-rings or graphite seals Fluorocarbon O-ring seals are easier to replace than graphite that deforms and flakes Graphite seals are used when inlet temperatures exceed 350C
Fluorocarbon O-ring Graphite O-ring for splitless liner Graphite O-ring for split liner
12/pk 10/pk 10/pk
5180-4182 5180-4173 5180-4168
P/N 5180-4182
158
GC Supplies
Inlet Liners Megabore Injection Port Liners for J&W Injection Port Conversion Kits
Direct Injection Liners
Minimizes the potential for sample backflash Most efficient transfer of the sample from the inlet to the column
Hot On-Column Liners

Inject samples directly into the column in a standard 26 gauge syringe
Flash Vaporization Megabore Liner

Can be packed in glass wool to minimize the amount of non-volatile residues reaching the column
Liners
Description Direct Injection Liner 1/4 in. Port Direct Injection Liner - 5890 Direct Injection Liner 1/4 in. Port Direct Injection Liner - 5890 Hot On-column Liner 1/4 in. Port Hot On-column Liner - 5890 Silanized Flash Vaporization Megabore Liner Length (mm) 220.0 85.0 220.0 85.0 220.0 85.0 220.0 Part No. 210-1064 210-1064-G 210-1063* 210-1063-G* 210-1066 210-1066-G 210-1070**
Hot On-column Direct Injection
GC and GC/MS
*These liners are not precut to fit specific injectors. They must be cut to the proper length before use. **This liner has no top restriction; it can be packed and cleaned easily.
Liner Ferrules for J&W Injection Port Conversion Kits

Description Graphite/Vespel Ferrule 6 mm, 1/4 in. Graphite Ferrule for 6 mm Mega/Split-Splitless Injection Port Liner, Double Taper Unit 10/pk 10/pk Part No. 500-1060 500-2066
Note: These liners are designed to fit J&W MegaBore conversion kits only and are not direct replacements for Agilent part numbers.
159
GC Supplies
Ferrules
Ferrules
Using the wrong ferrule or a worn out ferrule to seal your column connection can result in inconsistent and unreliable chromatography. An improper ferrule can cause leaks which allow air and other contaminants to enter the instrument through the column seal, causing major interference with column and detector performance. For optimum performance, ferrules should be replaced every time the column is replaced and when performing column maintenance. At Agilent, we offer a comprehensive selection of ferrules made of different materials and configurations for a PerfectFit with your instrument and a leak-free connection between the column and injector. Three main types of ferrules are used with capillary GC columns: graphite, Vespel and Vespelgraphite composites. Graphite ferrules can withstand temperatures as high as 450C, and Vespel and Vespel-graphite ferrules are rated to 280C and 350C respectively.
What is the Function Ferrules seal the connection of the column or liner to the system. The ideal ferrule provides a leak-free seal, accommodates various column outer diameters, seals with minimum torque, will not stick to the column or fittings, and will tolerate temperature cycling.
Why Replace
How to Minimize Problems General technique for installing ferrules:
GC and GC/MS
Signals that a ferrule is damaged include: background noise from oxygen diffusing into the system column bleed catalyzed by oxygen sample degradation loss of sample increase in detector signal/noise poor retention time reproducibility
dont overtightenfingertighten column nut, then use wrench to tighten a 1/4 turn maintain cleanliness bake out ferrules prior to use avoid contaminationfingerprints, oils inspect used ferrules with magnifier for cracks, chips, or other damage before reusing them change ferrules when new columns or injector/detector parts are installed
160
GC Supplies
Ferrules Ferrule Troubleshooting
Problems possible after changing ferrules
Normal Peaks Correct column positioning in both injection port and FID
Tailing Solvent Peaks Column positioned incorrectly in the injection port or possible ferrule particle in the carrier gas flow path
Wrong Peak Ratios Column positioned in the inlet (either too far or not far enough; verify 4-6 installation distance)
Ferrule Selection Recommendations

Ferrule/ Seal Type Upper Temperature Limit Usages Advantages Limitations
Graphite (100%)
450C
General purpose for capillary columns. Suitable for FID and NPD. Recommended for high temperature and cool on-column applications. General purpose for capillary columns. Recommended for MS or oxygen sensitive detectors. Most reliable leak-free connection. Isothermal operation. Can be reused or removed easily Excellent sealing material when making metal or glass connections.
Easy to use stable seal Higher temperature limit Can be removed easily Mechanically robust Long lifetime
Not for MS or oxygen sensitive detectors. Soft, easily deformed or destroyed Possible system contamination
GC and GC/MS
Vespel/Graphite 350C (85%/15%)
Not reusable Flows at elevated temperature Must retighten frequently Leaks after temperature cycle Flows at elevated temperature Must retighten frequently
Vespel (100%)
280C
Mechanically robust Long lifetime Can be reused or removed easily
161
GC Supplies
Capillary Column Ferrules Capillary Column Ferrules
Ferrule ID (mm) Column ID (mm) Unit Part No. General Purpose Graphite Ferrules (Short Ferrules)
0.5 1.0 0.4 0.8
0.1, 0.2, 0.25, 0.32 0.53 0.05-0.25 0.45, 0.53
10/pk 10/pk 10/pk 10/pk
5080-8853 5080-8773 500-2114 500-2118
85% Vespel, 15% Graphite Ferrules (Short Ferrules)
0.4 0.5 0.8
0.1, 0.2, 0.25 0.32 0.45, 0.53
10/pk 10/pk 10/pk
5181-3323 5062-3514 5062-3512
Preconditioned 85% Vespel, 15% Graphite Ferrules (Long Ferrules)*
0.3 0.4 0.5 0.8
0.1 0.1, 0.2, 0.25 0.32 0.53
10/pk 10/pk 10/pk 10/pk
5062-3507 5062-3508 5062-3506 5062-3538
100% Vespel High-Performance Ferrules (Short Ferrules)**
GC and GC/MS
0.4 0.5 0.8
0.1, 0.2, 0.25 0.32 0.45, 0.53
10/pk 10/pk 10/pk
5181-3322 5062-3513 5062-3511
Specialty Ferrules, 85% Vespel, 15% Graphite Two Hole
0.4 id holes 0.5 id holes

No Hole
0.1, 0.2, 0.25 0.32
10/pk 10/pk 10/pk
5062-3580 5062-3581 5181-3308
*These ferrules are recommended for use with GC/MS **These ferrules are recommended for use in isothermal analysis only.
162
GC Supplies
Column Nuts/Ferrules
Column Nuts
Description Short Nuts Universal column nut, 1/16 in. hex Finger-tight column nut for 0.53 mm columns* Finger-tight column nut for 0.32 mm columns and smaller* Blanking plug, finger-tight style 6850 column nut
Long Nuts
Unit 2/pk
Part No. 5181-8830 5020-8293 5020-8292 5020-8294
2/pk
5183-4732
MS interface column nut Column nut for GC/MS ferrules

Column Wrench
05988-20066 05921-21170
Column nut wrench, 1/4 in. and 5/16 in.
8710-0510
*For use with graphite ferrules only.
Helpful Hint: Always match short nuts with short ferrules and long nuts with long ferrules for a PerfectFit.
Straight Ferrules
Description 1/4 in. Teflon 1/4 in. graphite 1/8 in. graphite 1/8 in. 15% graphite/85% Vespel 1/16 in. Teflon 1/16 in. graphite 1/16 in. VG-2 Vespel, 40% graphite 6.4 mm Vespel 1/4 in. 15% graphite/85% Vespel 10/pk Unit 10/pk 10/pk 10/pk 10/pk 10/pk 10/pk 10/pk Part No. 0100-1378 0100-1324 0100-1325 0100-1332 0100-1375 0100-1326 0100-1379 0100-1104 0100-1331
GC and GC/MS
Reducing Ferrules
Description 1/8 to 1/16 in Vespel 1/8 to 1/16 in. VG-1 Vespel, 15% graphite 1/16 in. to 0.4 mm VG-2 Vespel, 40% graphite Unit 10/pk 10/pk 10/pk Part No. 0100-1342 0100-1344 0100-1381
163
GC Supplies
Column Union Supplies/Column Connectors
Column Union Supplies
Description Column ID Unit Part No.
Self-compensating capillary nut assembly* Split/splitless seal disk, gold plated stainless steel Fused silica column union assembly Quartz liner replacement for fused silica union Ferrule, reusable, Vespel Ferrule, reusable, Vespel Ferrule, reusable, Vespel 200, 250 m 320 m 530 m 10/pk 10/pk 10/pk
19258-20920 5182-9652 5182-0744 5182-0743 5182-0740 5182-0741 5182-0742
*The split/splitless seal disk (P/N 5182-9652) must be used with this assembly. Use Vespel ferrules only.
Column Connectors/Splitters
GC and GC/MS
Glass Capillary Column Connectors
Description
Glass Press-Fit Connector for:
Unit 10/pk 10/pk 10/pk 10/pk 10/pk 10/pk 10/pk 10/pk 5/pk 10/pk 10/pk 10/pk 5/pk 10/pk 25/pk 5/pk 25/pk
Part No. 5041-2178 5062-3553 5062-3552 5041-2176 5041-2175 5041-2173 5062-3556 5062-3554 5062-3550 5041-2174 5041-2172 5062-3555 5062-3551 5041-2171 705-0825 705-0905 705-0925 705-0903 500-1200
0.1 to 0.1 mm tubing 0.1 to 0.25 mm tubing 0.1 to 0.32 mm tubing 0.2 to 0.2 mm tubing 0.2 to 0.32 mm tubing 0.2 to 0.53 mm tubing 0.25 to 0.25 mm tubing 0.32 to 0.25 mm tubing 0.32 to 0.25 mm tubing 0.32 to 0.32 mm tubing 0.32 to 0.53 mm tubing 0.53 to 0.25 mm tubing
Glass Press-Fit Connectors
0.53 to 0.25 mm tubing 0.53 to 0.53 mm tubing Glass Union, universal 2-way Fused Silica Union, universal 2-way Fused Silica Union, universal 2-way Fused Silica Union, universal 3-way Polyimide sealing resin (5 grams)
164
GC Supplies
Glass Capillary Column Splitters
Description
Glass Press-Fit Splitter for:
Part No. 5021-1860 5062-3549 5041-2179 5021-1861
200 m columns 250 m columns 320 m columns 530 m columns

Glass Connector Trial Kit for Capillary Columns:
Kit contains 3 ea of 530 to 530 m, 530 to 320 m, 530 to 200 m, 200 to 320 m, 200 to 200 m
5062-3509
Quartz Column Connector P/N 5181-3395
Quartz Capillary Column Connectors/Splitters

Description Quartz column connector, 0.1 to 0.53 mm Quartz deactivated column connector Quartz splitter Quartz deactivated splitter Unit 5/pk 5/pk Part No. 5181-3395 5181-3396 5181-3397 5181-3398
GC and GC/MS
Mechanical Capillary Column Connectors

Description Connector, body and nut
Vespel Ferrules, for connecting:
Quartz Deactivated Splitter P/N 5181-3398
Unit
Part No. 5061-5801
0.2 to 0.53 mm id tubing 0.32 to 0.32 mm id tubing 0.32 to 0.53 mm id tubing Fused silica, undeactivated 530 m tubing, 10 m
2/pk 2/pk 2/pk
5061-5804 5061-5805 5061-5806 160-2530-10

Connector for Agilent Split/Splitless Injection Ports P/N 5061-5801
Graphpak Capillary Column Connectors

Column ID (mm) 0.32/0.25 0.53 Connector ID (mm) 0.4 0.7 Part No. 5021-7166 5021-7164
Graphpak Connector for Agilent Capillary Detector
Graphpak Connector for Agilent Capillary Detector Ports*
Graphpak Divider for Simultaneous Sampling to Two Capillary Columns
0.32/0.25 0.53
0.4 0.7
5021-7148 5021-7146
*Order ferrules in addition to connector to fit your column. Ferrules must be ordered separately.
165
GC Supplies
Graphpak Capillary Column Connectors (continued)
Column ID (mm) 0.2 0.32/0.25 0.53 Plug ferrule
Graphpak Divider for Simultaneous Sampling
Connector ID (mm) Unit 0.3 0.4 0.7 10/pk 10/pk 10/pk 10/pk 5/pk
Part No. 5021-7136 5021-7137 5021-7134 5021-7133 5062-3525
Ferrules for Graphpak Connection Systems
Replacement Graphpak column nut
Graphpak Connector for Agilent Capillary Injection Ports
Column ID (mm) 0.2 0.32/0.25 0.53
ID (mm) 0.3 0.4 0.7
Part No. 5021-7169 5021-7170 5021-7168
*Order ferrules in addition to connector to fit your column. Ferrules must be ordered separately.
Injector Transfer Line Splitter Union
Capillary Column Splitters

Description
Fixed inlet splitter
Tubing ID (mm) 0.22 mm 0.32 mm 0.53 mm
Part No. 5181-3390 5181-3391 5181-3392
GC and GC/MS
Column 1
Column 2
Fixed Inlet Splitter
Fixed outlet splitter (not for use with 0.53 mm)
1:1 ratio splitter

Detector 1 Detector 2
0.22 mm to 0.22 mm 0.22 mm to 0.15 mm 0.21 mm to 0.11 mm
0101-0594 5181-3389 0101-0595
1:5 ratio splitter 1:10 ratio splitter
Two-hole replacement ferrules (10/pk)

Transfer Lines
0.3 mm 0.4 mm 0.5 mm
5181-3388 5062-3580 5062-3581
Splitter Union
Column
Inlet
Variable outlet splitter for capillary columns
Fixed Outlet Splitter
Outlet splitter system includes metal body and 5 m 0.22 mm id transfer line with 0.4 mm 2-hole ferrule.
5181-3393
Variable Outlet Splitter P/N 5181-3393
166
GC Supplies
Valves and Loops
Valves and Loops

Gas Sampling General-Purpose Valves Compatible with the 5890/6890
Description 6-port replacement valve WE series, 400 psi, 225C 6-port replacement valve WE series, Hastelloy C, 400 psi, 225C 10-port replacement valve WE series, 400 psi, 225C 6-port replacement valve WT series, 300 psi, 350C 10-port replacement valve WT series, 300 psi, 350C Part No. 5062-9508 5062-9509 5062-9510 0101-0584 0101-0585
General Purpose Gas Sampling Valves
10-port replacement valve WE series, Hastelloy C, 400 psi, 225C 5062-9511
Liquid Sampling General-Purpose Valves

Description 0.2 L replacement valve UWP series, 1,000 psi, 75C 0.5 L replacement valve UWP series, 1,000 psi, 75C 1.0 L replacement valve UWP series, 1,000 psi, 75C 0.5 L replacement valve UW series, 5,000 psi, 75C Part No. 0101-0636 0101-0637 0101-0638 0101-0639
GC and GC/MS
Parts for Interfacing Capillary Columns to W Series Valves

Description SS bulkhead ZDV union (for interfacing 530 m columns with 1/16 in. transfer line) SS bulkhead ZDV union (for interfacing 320 m columns with 1/16 in. transfer line) Polyimide 1/16 in. ferrule Polyimide liner for 530 m columns Polyimide liner for 320 m columns 1/16 in. SS counterbored nut for use with above liners Installation tool for liners Part No. 0100-1515 0100-1527 0100-1512 0100-1513 0100-1514 0100-1511 18900-20850
General Purpose Liquid Sampling Valves
167
GC Supplies
Valves and Loops
Valve-Related Parts
Description 1/16 in. SS nut (10/pk) 1/16 in. SS ferrule (10/pk) Metering valve (for LSVs as a sample-out restrictor or as a flow-balancer for 10-100 mL/min) Air actuator Solenoid valve for controlling actuator Solenoid valve for controlling actuator (for GCs after serial number 3223A43573) Straight metering valve, 1/16 in. SS Angle metering valve, 1/16 in. SS Part No. 5181-1291 5181-1292 0101-0355
Micrometering valve (for flow balancing gas flows of 2-50 mL/min) 0101-0633 19325-60660 05890-61090 05890-61095 0101-0355 0101-0403
GC Loops Compatible with Agilent 5890/6890 (Includes Loop, Nut and Ferrule, 1/16 in.)
Description 0.25 cc 0.50 cc 1.00 cc 2.00 cc 5.00 cc 10.00 cc A. Tube, 1/16 in. SS, 280 mm long Tube, 1/16 in. SS, 400 mm long Tube, 1/16 in. SS, 375 mm long Tube, 1/16 in. SS, 560 mm long B. Tube, 1/16 in. with 1/8 in. flare, SS, 360 mm long Tube, 1/16 in. with 1/8 in. flare, SS, 520 mm long C. Tube, 1/16 in. with 1/8 in. bulkhead fitting, 520 mm long Part No. 0101-0303 0101-0282 0101-0299 0101-0300 0101-0301 0101-0302 18900-20250 18900-20280 18900-20281 18900-20300 1530-2163 1530-2167 07675-80050
GC and GC/MS
Tube, nickel, 1/16 in. with 1/8 in. bulkhead fitting, 460 mm long 18900-80255
Replacement Rotors for Gas Sampling Valves with External Loops

A B C
Description 6-port replacement rotor WE series, 400 psi, 225C 10-port replacement rotor WE series, 400 psi, 225C 6-port replacement rotor WT series, 300 psi, 350C 10-port replacement rotor WT series, 300 psi, 350C
Part No. 5181-7459 5181-7460 1535-4952 1535-4954
168
GC Instrument Parts and Supplies

Purge and Trap Supplies
GC Instrument Parts And Supplies

When it comes to instrument replacement parts for your Agilent GCs, dont settle for an imitation, use genuine Agilent parts. Our inlets are designed, tested and manufactured by Agilent engineers to work perfectly with our instruments and give you superior performance. Plus we supply every replacement part you would ever need for your entire system, not just a few select parts. So keep your instrument operating at its very best with Agilent consumables and replacement partsthe PerfectFit for optimum performance.
Tekmar 3100 and Agilent 7695A Purge and Trap Supplies

Glassware
Fritless Sparger: Compared to a frit sparger, the fritless sparger may be the better choice when a water sample has a tendency to foam. This sparger is not appropriate for soil samples, which tend to clog the capillary tube. Available in 1/2 and 3/4 in. mount sizes. Disposable (Needle) Tubes: The most practical choice for soil (and other solid or semisolid) samples, these tubes permit easy sample loading and inexpensive disposal. Available only in 3/4 in. mount size, a 3/4 in. tube can be used on the 7695A Purge and Trap concentrator with the adapter (to convert from 7695A Purge and Trap 1/2 in., to this 3/4 in. size). Needle Sparger with Foam Trap: This sparger is designed for samples that foam, particularly if the foaming cannot be controlled by a fritless sparger. Available only in 1/2 in. mount size. Frit Sparger: This is the most efficient design in terms of purging and it is the standard choice for clean drinking water. Available in 1/2 in. and 3/4 in. mount sizes. Note: The 7695A Purge and Trap concentrator has a 1/2 in. mount, and the automatic sampler has a 3/4 in. mount. An adapter is available to mount 3/4 in. glassware on the 7695A Purge and Trap concentrator, but there is no adapter for using 1/2 in. glass on the automatic sampler.
7695 Purge and Trap
GC and GC/MS
169

Agilent 7695A and Tekmar 3100 Purge and Trap Supplies
Description
Glassware Supplies 1/2 in. Mount
Part No. 5182-0852 5182-0846 5182-0851 5182-0845 5182-0850 5182-0844 5182-0849 5182-0796 5182-0848 5182-0795 5182-0847 5182-0794
5 mL frit sparger (glassware only) 5 mL frit sparger kit with fittings 25 mL frit sparger (glassware only) 25 mL frit sparger kit with fittings 5 mL fritless sparger (glassware only) 5 mL fritless sparger kit with fittings 25 mL fritless sparger (glassware only) 25 mL fritless sparger kit with fittings 5 mL needle sparger (glassware only) 5 mL needle sparger kit 25 mL needle sparger (glassware only) 25 mL needle sparger kit
Glassware and Corresponding Needles 3/4 in. Mount
5 mL frit sparger Needle, 8 in.
5182-0893 14-3195-053TMR 5182-0894 5182-0789 5182-0895 5182-0789 12-0507-024TMR 14-5186-153TMR
GC and GC/MS
25 mL frit sparger Needle, 9 1/8 in. 5 mL fritless sparger Needle, 9 1/8 in. 150 mm disposable needle tube sparger Needle, pointed 8 in.
Sample Handling Supplies
Sample valve, 3-port assembly

Miscellaneous Supplies
5182-0793 5182-0792 14-5681-102 5182-0789 5182-0788 5182-0746 9301-1185
Female luer connector for sample valve Purge line assembly Sample needle, for 25 mL frit or fritless sparger Needle for 5 mL frit or fritless sparger Conversion kit adapter for use with 3/4 in. tube on 1/2 in. mount G1900A concentrators 5 mL sample syringe with luer connector
170

Tekmar 3100 and Agilent 7695A Purge and Trap Autosampler Supplies
Glassware Assembly (3/4 in.)
Item Description 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 Sample drain line assembly Sample valve assembly, 3 port Female luer connector for sample valve Sample needle ferrule Sample needle nut Nut Ferrule Union 3/4 in. ferrules 3/4 in. nut Glassware Sample purge line top Sample purge line bottom 1/4 in. to 1/16 in. union Ferrules Nut Part No. 14-3137-200TMR 14-0036-050TMR 14-0216-016TMR 14-3123-016TMR 14-3124-016TMR 14-2087-016TMR 14-3979-016TMR 14-3914-016TMR 14-0739-016TMR 14-0857-016TMR (see table below) 14-3197-100TMR 14-3198-100TMR
1 2 3 4 5 6 7 12 8 13 9 14 10 15
11 11
GC and GC/MS
14-2261-016TMR 14-3965-016TMR 14-2087-016TMR
7695A and Tekmar 3100 Purge and Trap Autosampler Traps

Description Trap, Tenax Trap, Tenax/silica gel/charcoal Trap, Tenax/charcoal Trap, OV-1/Tenax/silica gel/charcoal Trap, OV-1/Tenax/silica gel Trap, Vocarb 3000 Trap, Bocarb 4000 Trap, BTEX Heater assembly trap Hydrocarbon trap assembly Part No. 5182-0783 5182-0781 5182-0780 5182-0779 5182-0778 5182-0775 5182-0774 5182-0773 5182-0772 5182-0771
171

Tekmar AQUATek 70 Purge and Trap Autosampler Supplies
Description
Sample Loops
Unit
Part No.
5 mL sample loop, AQUATek 70 25 mL sample loop, AQUATek 70 20 mL sample loop, AQUATek 70

Glassware for 3000/2000 Agilent 7695 (glassware only)
5182-9689 5183-4733 5183-4734
Sparger, 25 mL frit Sparger, 5 mL frit Sparger, 5 mL fritless Sparger, 25 mL fritless

Vials
5183-4735 5183-4736 5183-4737 5183-4738
Screw caps for 40 mL vials
24/pk
5183-4744
Miscellaneous Purge and Trap Supplies

Description Spare/Alternative transfer line tubing, passivated nickel, for 0.53 mm id fused silica or packed columns, 72 in. long (tubing only) Chemical Standard, Method 501 1 mL ampoule (0.2 mg in MeOH) dichlorobromomethane, dibromochloromethane
Interface Cables
Part No. 5181-8822
GC and GC/MS
8500-5901
Agilent 5890 to 7695 and TMR-3100 P&T (P/N G1900A-010) Agilent 5890/MSD to 7695 and TMR-3100 P&T (P/N G1900A-011)
5183-4745 5183-4746
172

Automatic Liquid Sampler Supplies

Description
Miscellaneous Autosampler Supplies
Unit
Part No.
4 mL wash vial with screw caps Diffusion caps for 4 mL vials Septa for 4 mL vial 4 mL wash vial with fill marking, caps Screw for mounting syringe Quadrant kit (4 tray sections) 7673 Basic Supply Kit: contains: 10 mL syringes (6/ea), 23/26 gauge needles, 4 mL vials with diffusion caps (144/pk), 2 mL automatic sampler vials with screw caps (1,000/pk) GC septa (25/pk), Vial racks (5/pk)
144/pk 12/pk 144/pk 25/pk
9301-0723 07673-40180 9301-1031 5182-0551 07673-20570 18596-40015 07673-60840
Note: For crimping and decapping tools, see page 37.
GC and GC/MS
18587A Bar Code Reader Labels

Description
Labels numbered (1,000/roll)
Part No.
1 to 1,000 1,001 to 2,000 2,001 to 3,000 3,001 to 4,000 4,001 to 5,000 5,001 to 6,000 Label applicator
5958-9450 5958-9441 5958-9442 5958-9443 5958-9444 5958-9445 18587-60560

Bar Code Reader Labels
Helpful Hint: For a complete selection of Vials and Caps, see the General Chromatography section of this catalog.
173

Headspace Supplies
19395A and 7694 Headspace Analysis Supplies

Agilent 19395A Miscellaneous
Description Silicone oil (oil bath capacity = 3.5 pt), 1 pt Thermosetting tape Headspace evaluation sample: 2% t-butyldisulfide, 10% nonane, 2% 1,2-dichlorobenzene, 2% nitrobenzen, Solvent: dodecane Part No. 8500-0010 0460-0186 8500-4328
Helpful Hint: Don't forget about your vials and caps for Headspace. See the General Chromatography section of this catalog.
Agilent 7694 Sampler Supplies

7694 Headspace Sampler
Description Transfer line, Silcosteel, 1 m Needle, transfer line, Silcosteel Needle, transfer line, Silcosteel Transfer line nut Transfer line ferrule
Part No. 301-211-HSP 301-222-HSP 301-221-HSP 19258-20830 19258-20870 301-017-HSP 300-301-HSP 321-055-HSP 321-056-HSP 301-220-HSP 301-015-HSP 301-016-HSP 321-002-HSP 330-086-HSP 330-055-HSP 425-553-HSP 301-169-HSP 301-212-HSP 321-058-HSP 321-059-HSP 301-011-HSP 301-152-HSP 301-013-HSP 325-045-HSP 425-554-HSP
GC and GC/MS
Oven adaptor for 10 mL vials Tray adaptors for 10 mL vials, (25/pk) Sample loop, 1 cc, nickel Sample loop, 3 cc, nickel Needle assembly vial probe, Silcosteel Needle for transfer line, 0.4 mm id, 0.8 mm od, nickel Needle for transfer line, 0.25 mm id, 0.5 mm od, nickel Restrictor, stainless steel Pressure regulator Flow controller Solenoid valve, pressure Tube, needle, 6-port valve, nickel Tube, needle, 6-port valve, Silcosteel Sample loop, 1 cc, Silcosteel Sample loop, 3 cc, Silcosteel Transfer line, 80 cm, nickel Transfer line, 1 m, nickel Needle assembly (vial probe) Zero dead volume union Solenoid valve-vent
174

Headspace Supplies/6890/6850 Split/Splitless Inlet Supplies
Agilent 7694 Sampler Supplies (continued)
Description Bulkhead fitting kit Strain relief Tube, vent-valve stainless steel Vial, 10 mL Headspace OQ/PV standard Sensor tube, 125 mm PTFE Union FF 6MB 5 piece set Union T6 MB 5 piece set, brass Union T5 MA Nut 5 mm 100/pk Unit Part No. 325-024-HSP 301-205-HSP 301-170-HSP 5182-0838 5182-9733 321-057-HSP 325-062-HSP 325-132-HSP 325-185-HSP 325-455-HSP
6890/6850 Split/Splitless Inlet Supplies

Split/Splitless Inlet Supplies*
Item Description 1 2 3 Septum retainer nut for headspace Septum retainer nut Septa, bleed temperature optimized** Insert Weldment Standard manual pneumatics Original standard EPC using 1/4 in. split vent filter Similar to G1544-60575 except allowed insertion for 1/4 in. chemical filters to clean carrier gas for ECD operation S/SL insert weldment. Used with large charcoal canister type filter, for 6890/6850 Similar to G1544-60575 except carrier lines separated for interface to valved systems of a G1540A instrument S/SL insert assembly for 1540A with valved system option. This insert assembly uses the large charcoal canister split vent filter, for 6890/6850 4 Graphite O-rings for split liner Graphite O-rings for splitless liner Fluorocarbon elastomer O-ring 12/pk 10/pk 10/pk 50/pk Unit Part No. 18740-60830 18740-60835 5183-4757 19251-60575 G1544-60575 G1544-80580
6 4 5 1 2 3
GC and GC/MS
G1544-60585 G1580-60575
G1580-60585
7 8 9
5180-4168 5180-4173 5180-4182
10
11 12 13 14
6890/6850 Series GC Split/Splitless Inlet
175

6890/6850 Split/Splitless Inlet Supplies
Split/Splitless Inlet Supplies* (continued)
Item Description
1 2 3 4 6 5
Unit 25/pk 25/pk
Part No. 5183-4647*** 5183-4702 5181-3316*** 5183-4696 G1544-60610 G1544-80530 G1544-80550 G1544-20590 18740-20880 18740-20885 5182-9652
Liner, split, low pressure drop, glass wool, single taper, deactivated Liner, splitless, Single taper, deactivated liner, 900 L
Split trap vent kit w/repl. cartridge, optional Replacement cartridge for P/N G1544-60610 Split vent trap assembly
7 8
Retaining nut SS seal Gold-plated seal Gold-plated seal with cross
9 10
7 8 9
Washer, 0.375 in. od Reducing nut Insulation (requires 3) Lower insulation cover Ferrule **** Column nut for 6890 Column nut for 6850 Angled wrench for split/splitless inlet Split/splitless injection port kit contains:
12/pk
5061-5869 18740-20800 19243-00067 19243-00070
11 12 13 14
GC and GC/MS
10
2/pk 2/pk
5181-8830 5183-4732 19251-00100 5181-8838 18740-20885 19251-60540 5062-3587 5180-4182 5181-8818 5183-4757 5180-4153
11 12 13 14
6890/6850 Series GC Split/Splitless Inlet
Gold plated seal, 3 ea. Liner, split, straight, glasswool, non-deactivated, 4 ea. Liner, splitless, single-taper, glasswool, deactivated, 2 ea. 1/4 in. Viton O-ring, 12/pk Liner, direct, 2 mm id, deactivated Bleed/Temp optimized inlet septa 11 mm, 50/pk Capillary inlet cleaning wires, 5/pk
*For a complete parts breakdown, see the 6890/6850 Series GC Instrument User and/or Service Manuals. **For a complete selection of septa, see pages 149153. ***For complete list of liners, see pages 157158. ****For a complete selection of capillary column ferrules, see pages 162163.
Gold Plated Seal P/N 18740-20885
Helpful Hint: The new optional replacement split vent trap, G1544-60610, can replace the standard 1/4 inch trap G1544-80550 using the same type of connections as the standard trap and is installed in the same location of the pneumatics carrier.
Capillary Split Vent Trap Assembly P/N G1544-80550
Optional Capillary Split Vent Trap Assembly P/N G1544-60610
176

6890 PTV Inlet Supplies
Programmable Temperature Vaporizing (PTV) Inlet

PTV Inlet
Item Description 1 2 3 4 5 6 7 8 Septumless head Septum head Septum nut PTV inlet assy PTV LCO2 cooling jacket PTV LN2 cooling jacket Silver seal Graphpak inlet adapter 0.20 mm 0.25-0.33 mm 0.53 mm 9 Ferrules for Graphpak inlet 0.20 mm 0.25 mm 0.32 mm 0.53 mm 10 11 Split nut for inlet adapters PTV insulation block PTV cryo insulator (not shown) Teflon ferrule (needle seal) Kalrez seal Valve body Pressure spring Viton seal Sealing element CO2 Cryo inline filter Service kit for septumless head contains Kalrez seal, valve body, and pressure spring Graphpak 3D ferrules Installation tool for 3D ferrules 5/pk 10/pk 10/pk 10/pk 10/pk 5/pk Column ID Unit Part No. G2617-60507 G2618-80500 18740-60835 G2617-60506 G2617-60508 G2619-60501 5182-9763 5182-9754 5182-9761 5182-9762 5182-9756 5182-9768 5182-9769
4 1 3 2
GC and GC/MS
5182-9770 5062-3525 G2617-20510 G2617-60510 5182-9748 5182-9759 5182-9757 5182-9758 5182-9775 5182-9760 3150-0602 5182-9747
PTV Inlet
5, 6
7 8
9 10
11
5182-9749 G2617-80540
PTV Liners
Description PTV Liner Single Baffle, 2 mm id 180 L volume, deactivated, glasswool PTV Liner Single Baffle, 2 mm id 200 L volume, deactivated PTV Liner Multi Baffle, 1.5 mm id 150 L volume, deactivated PTV Liner Fritted Glass, 1.5 mm id 150 L volume, deactivated www.agilent.com/chem Part No. 5183-2038 5183-2036 5183-2037 5183-2041
177

6890 Cool On-Column Inlet Supplies

Like the split/splitless inlet, the cool on-column inlet may be used with manual or automated injection. Required inlet hardware for manual injection can include either a septum nut and septum or cooling tower and duckbill septum (indicated on the part shown), along with the spring and insert. Automated injection requires the septum nut and septum, spring, and insert.
6890 Series GC Cool On-Column Inlet Supplies*

Item Description
Automatic injection
Unit
Part No.
1a 1b 2
1a 1b 1c 1d
Septum nut base for 320 mm assembly Septum nut base for 530 mm assembly Advanced green 5 mm through hole septa** BTO 5 mm through hole septa** 50/pk 50/pk
19245-80521 G1545-80520 5183-4760 5183-4758
Manual injection
1c 1d
2 3 4
Cooling tower assembly Duckbill septum 10/pk 6/pk
19320-80625 19245-40050 19091-63000 9301-0658
Fused silica syringe needles Syringe barrel for use with fused-silica needles,10 L
GC and GC/MS
Common Supplies
3 4
Spring Inserts For 200 m columns (one ring) For 250 m columns (six rings) For 320 m columns (five rings) For 530 m columns (no rings) For 530 m Al clad columns (four rings)
19245-60760 19245-20510 19245-20515 19245-20525 19245-20580 19245-20780 5080-8853 2/pk 5181-8830
5
5 6
Ferrule*** Column nut
6890 Cool On-Column Inlet
*For a complete parts breakdown, see the 6890 Series GC Instrument User and/or Service Manuals. **For a complete selection of septa, see pages 149153. ***For a complete selection of ferrules, see pages 162163.
178


Be sure to select a needle, septum nut, and insert based on the inside diameter of the column selected. Use the following tables to make this determination for automatic and manual injections.
Automatic or Manual Injection Using Stainless Steel Needles

Column Type and ID Fused silica Needle Part No.* Price Septum Nut** Part No.* Price Insert Part No.
530 m
5182-0832
$27
G1545-80520
$58
19245-20580
320 m
5182-0831
43
19245-80521
57
19245-20525 (5 rings)
Programmable COC, Septum Nut, 250/320 m P/N 19245-80521
250 m
5182-0833
43
19245-80521
57
19245-20515 (6 rings)
200 m
Use cooling and tower duckbill septum
19245-20510 (1 ring)
Programmable COC Septum Nut, 530 m P/N G1545-80520
GC and GC/MS
Aluminum clad
530 m
5182-0832
27
G1545-80520
58
19245-20515 (4 rings)
*All needles use 5 L syringe barrel, P/N 5182-0836. **Use a solid septum, P/N 5181-1261, for manual injection. Use through-hole septum, P/N 5181-1260, for automatic injection.
Column/Retention Gap Installation Consumables

Item Description Column nut 250 m graphite/Vespel ferrule 320 m 0.5 mm graphite/Vespel ferrule 250 m retention gap (one 5 m piece) 320 m retention gap (one 5 m piece) 530 m retention gap (one 5 m piece) Quartz deactivated column connector fits 0.18-0.53 mm 5/pk Unit 2/pk 10/pk 10/pk Part No. 5181-8830 5181-3323 5062-3514 160-2255-5 160-2325-5 160-2535-5 5181-3396
179

6890/6850 Packed Port Inlet Supplies
6890/6850 Packed Port Inlet Supplies

Selecting Adapters and Liners for Packed Inlets
1 2 3 4
For purged-packed inlets, adapter selection is dependent on the type of column used: 1/4 in. packed, 1/8 in. packed, or 0.53 mm. It allows the columns to be connected to the inlet. Glass liners are often used with metal adapters to reduce reactivity and trap nonvolatile residues. Liners are installed from the top of the inlet and should be installed before the column.
6890 Series GC Packed Port Supplies*

Item Description 1 2 3 4 5 6 7 Septum nut Septa Bleed temperature, optimized,** 11 mm Top insert weldment Viton O-rings Glass liner, disposable Glass liner, disposable/deactivated
Liner
Unit 50/pk 12/pk 25/pk 5/pk 10/pk 10/pk
Part No. 18740-60835 5183-4757 19243-80570 5080-8898 5080-8732 5181-3382 5080-8774 5180-4105
Vespel/graphite ferrules, 1/4 in. id Tubing nut, 1/4 in. brass
Adapters with glass liners
GC and GC/MS
8
5
0.53 mm column adapter 1/8 in. column adapter 1/4 in. column adapter
19244-80540 19243-80530 19243-80540 19243-00067 19234-60720 2/pk 5181-8830
6 7 8 1/4 in. Vespel/ graphite ferrule 1/4 in. nut 1/8 in. or 1/4 in. stainless steel liner
9 10 11
Upper insulation Nut warmer cup with insulation Column nut for 0.53 mm column
Inside of Oven 9 10
*For a complete parts breakdown, see the 6890 Series GC Instrument User and/or Service Manuals. **For a complete selection of septa see pages 149153.
Installation Matrix for Packed Metal Columns

11
6890 Series GC Packed Port Inlet
Inlet or Detector
1/4 in. Columns Where to Install
Key Points
1/8 in. Columns Where to Install
Key Points
Purged packed inlet Adaptable NPD* or FID ECD TCD
1/4 in. liner
1/8 in. liner
1/4 in. adapter Remove or install 1/8 in. adapter (P/N 19231-80530) adapter as desired (P/N 19231-80520) Detector fitting 1/4 in. adapter (P/N G1532-20710) Remove adapter if necessary 1/8 in. adapter (P/N 19301-80530) Detector fitting Remove adapter if necessary
*Do not remove plugs from your NPD until you are ready to connect the column and operate the detector.
180

6890/6850 Detector Supplies
6890/6850 Series GC Detector Supplies

Detectors available for use with 6890 and 6850:
Flame ionization detector (FID) for packed or capillary columns Thermal conductivity detector (TCD) Mass-selective detector (MSD)
6890 only:
Electron capture detector (ECD) Nitrogen-phosphorus detector (NPD) for packed or capillary columns
GC Detector Specifications
GC/ Detector Max Op Temp Sensitivity (MDL) Dynamic Range Makeup Gas Types Selectivity
6890 GC/FID and 6850 GC/FID 6890 GC/NPD
450C 375C 400C
<5 pg carbon/sec as propane using N2, 0.2794 mm jet <0.4 pg N/sec, <0.2 pg P/sec with azobenzene/ malathion mixture <400 pg propane/mL He <0.04 pg/sec lindane
Linear: <10%, with N2 and 0.2794 mm jet >105 N, >105 P with azobenzene/ malathion mixture Linear: 105 (5%) 104 with lindane
N2 H He
not applicable
35000 to 1 gN/gC 75000 to 1 gN/gC not applicable
6890 GC/TCD and 6850 GC/TCD 6890 GC/ECD
400C 375C 400C
He N2 He N2 Ar He N2 Ar
GC and GC/MS
6890 GC/MSD 6850 GC/MSD
320C (Interface)
Full scan: 10:1 RMS on 10 pg hexachlorobenzene (splitless mode) Selected monitoring: 10:1 RMS on 200 pg hexachlorobenzene (splitless mode)
Linear: 104 Total ADC: 106
not applicable
Note: All 6890 Series GC detectors are available with manual or electronic regulation of gases; and all detectors, both manual and electronic, include electronic ON/OFF gas switching from the 6890 keyboard.
181

6890/6850 FID Supplies
6890/6850 Flame Ionization Detector (FID) Supplies

Item Description
1
Unit
Part No. 19231-21050 19231-20940
1 2 3 4
5
PTFE chimney (optional)** Collector nut Spring washer Ignitor castle Optional Hastelloy Ignitor Castle* Ignitor glow plug assembly Collector insulator Collector body FID collector assembly 1 Detector insulation assembly FID collector cleaning brush Collector Housing FID retainer nut wrench (5880, 5890, 6890) 2/pk 10/pk
2 3
5181-3311 19231-20910 19231-21060 19231-60680 G1531-20700 G1531-20690 G1531-60690 G1531-20700 8710-1346 G1531-20740 19301-00150 8710-1561 5182-3450 18710-20119 18710-60170 19231-60680 19244-80560 19301-60660 5180-4150 5180-4152 19301-20720 5180-4153 9301-0985
4 8 6 7
5 6 7 8
GC and GC/MS
1/4 in. Nut Driver for FID jet-drilled shaft FID supplies kit-Includes: Jet, packed standard, 3 each FID performance evaluation sample kit, 2 each Ignitor glow plug assembly, 2 each Jet, cap. series 530 m, 3 each FID flow measuring insert, 2 each Cleaning wires for 0.03 in. id jet Cleaning wires: 0.018 in. id/530 m jet, 1/pk Wire, jet cleaning, 5 each Capillary inlet cleaning wires Detector cleaning kit
6890 Series GC FID Detector
Collector Body P/N G1531-20690
*Hastelloy components may be used when analyzing highly corrosive materials. **Not included in assembly. 1 Does not include Hastelloy component.
FID Jets
Description
Jets for capillary dedicated FID
Length (mm) 48 48
Part No. G1531-80560 G1531-80620
Capillary jet (0.011 in./0.29 mm id tip) High-temperature jet (SimDIS 0.018 in. id tip)
182

6890/6850 FID Supplies
FID Jets (continued)
Description
Adaptable FID
Length (mm) 61.5 63.5 63.5 61.5
Part No. 19244-80560 18710-20119 18789-80070 19244-80620

FID Jet
Capillary 0.53 mm jet (0.011 in. id tip) Packed (0.018 in. id tip) Packed (0.030 in. id tip) 0.53 mm high temp (0.018 in. id tip) for SimDIS
FID Consumables
Description FID flow measuring insert FID ignitor cable FID Performance evaluation sample kit, 0.03% C14, C15, and C16 normal alkanes in hexane O-rings Cleaning wire Jet cleaning wire for 0.03 in. id jet 5/pk Jet cleaning wire for Series 530 mm and 0.018 in. id jet 5/pk 12/pk Unit Part No. 19301-60660 G1531-60680 18710-60170 5080-4978 19301-20720 5180-4150 5180-4152
GC and GC/MS
Cleaning Your FID Jet

It is often more convenient to replace dirty jets than to clean them, especially if they have been badly contaminated. However, if you decide to clean a jet, be careful not to scratch the jet internally; scratches will ruin the jet. To be on the safe side, you may want to clean the jet using an aqueous bath only. To do this you will need the following materials: Small ultrasonic cleaning bath Aqueous detergent GC-grade methanol in a Teflon wash bottle Flame detector cleaning kit (P/N 9301-0985) Dry, filtered compressed air or nitrogen Forceps or tweezers 1. Run a cleaning wire through the top of the jet. Run it back and forth a few times until it moves smoothly. Be careful not to scratch the jet. 2. Fill the ultrasonic cleaning bath with aqueous detergent, and place the jet in the bath. Sonicate for five minutes. 3. Use a jet reamer to clean the inside of the jet. 4. Sonicate again for five minutes. Note: From this point on, handle the parts only with forceps! 5. Remove the jet from the bath and rinse it thoroughly first with hot tap water and then with a small amount of the methanol. 6. Blow the jet dry with a burst of the compressed air or nitrogen, and then place the jet on a paper towel and allow it to air dry.
FID Cleaning Kit P/N 9301-0985
183

6890 NPD Supplies
6890 Nitrogen Phosphorus Detector (NPD) Supplies

The NPD for the 6890 GC features a ceramic bead selective for nitrogen and phosphorus compounds. All Agilent NPD beads are preconditioned and come with a proof-of-performance chromatogram. The redesigned NPD bead is self-aligning for easy installation.
NPD Ceramic Bead
Like the FID, there are two types of NPD is available. The capillary optimized NPD is only used with capillary columns and the adaptable NPD fits packed columns and can be adapted to fit capillary columns.
2
6890 NPD Supplies

Item Description 1 2 Screws, M3 x 0.5 x 8 mm (Pozidriv) NPD white ceramic bead assembly* NPD black ceramic bead assembly** 3 4 5 6 7 8 9 10 11 12 13 14 Lid weldment Metal C-ring, top Alumina insulator, upper Collector funnel Alumina insulator, lower Metal C-ring bottom Screw, M4 x 10 mm J-Clamp Screw, M4 x 10 mm NPD interconnect assembly Mounting pallet
Jets
Part No. 0515-0655 G1534-60570 5183-2007 G1534-80510 0905-2580 G1534-40020 G1534-20530 G1534-40030 0905-1284 0515-2495 1400-0015 0515-2495 G1534-60610 G1531-40020 G1531-80560 19244-80560 18710-20119
G1534-80500
9 10 4 5 6 12
11
GC and GC/MS
13 14
15 16
Fused silica capillary (0.011 in.), dedicated, 48 mm length Packed capillary (0.011 in.), 61.5 mm length Packed column (0.018 in.), 63.5 mm length 15
Base weldment, Capillary NPD
17
18
Base Weldment, Packed NPD 16 Lid stop NPD Ceramic Insulator Kit includes items 4, 5, 7, and 8
G1534-80540 G1534-20590 5182-9722
*This bead is more sensitive but exhibits some tailing for phosphorous compounds. Quantity discounts available. **The black bead is potentially a little less sensitive but does not exhibit peak tailing and typically has longer lifetime.
6890 Series GC NPD Detector
184

6890 NPD/6890/6850 TCD Supplies
Item Description 17
Column adapters for packed NPD
Unit
Part No. 19244-80610 19231-80520 19231-80530 19234-60720
Capillary column 1/8 in. packed column 1/4 in. packed column 18 Nut warmer cup with insulation 1/4 in. Vespel ferrule 1.0 mm graphite ferrule 0.5 mm graphite ferrule 1/4 in. brass tubing nut Column nut NPD chemical sample kit solution of 0.65 ppm azobenzene, 1000 ppm octadecane, 1 ppm malathion in isooctane, 3 ampoules 10/pk 10/pk 10/pk 10/pk 2/pk
5080-8774 5080-8773 5080-8853 5180-4105 5181-8830 18789-60060
6890/6850 Thermal Conductivity Detector (TCD) Supplies

Item Description 1 2 3 4 5 TCD replacement cell TCD cap column adapter seal TCD cap column adapter Graphite ferrules, 1.0 mm id Graphite ferrules, 0.5 mm id Capillary column nut, for 6890 Capillary column nut, for 6850
Miscellaneous hardware for packed columns
Unit 10/pk 10/pk 10/pk 2/pk 2/pk 10/pk 10/pk 10/pk 10/pk
Part No. G1532-60675 18740-20950 18740-20960 5080-8773 5080-8853 5181-8830 5183-4732 5080-8774 0100-1332 5180-4103 5180-4105 G1532-20710 18711-60060
Agilent GC TCD
2 3 4 1
GC and GC/MS
Vespel/graphite ferrules, 1/4 in. id Vespel ferrules, 1/8 in. id Tubing nut, 1/8 in. brass Tubing nut, 1/4 in. TCD packed column adapter TCD chemical sample kit, 3 ampoules, 0.5 mL, solution of 0.3% C14, C15, and C16, normal alkanes in hexane
185

6890/6850 TCD Supplies TCD Column Ferrule Sealing System
Beginning in early 1999 the TC detector for the 6890 Series GC began using a new method of sealing the column into the base of the detector. The new fitting, which reduces noise slightly, forms a better seal without air leaks. It may still require retightening after wide temperature programming ranges. This new ferrule system improves the analytical results for samples that may react with oxygen. It consists of a brass back ferrule, a graphite/Vespel front ferrule, which is available in three different hole sizes and a no hole version. The same size brass back ferrule is used for all column sizes and is reusable over a long lifetime. Step 1: The column is placed through the nut first and then the back ferrule. Step 2: The front ferrule is placed next and must have the tapered end towards the detector (just like a standard ferrule) Step 3: The protrusion in the front ferrule must enter into a matching recess in the back ferrule. Tighten the fitting until it holds the column enough to resist a pull on the column to remove it. Step 4: Tighten the fitting until it holds the column enough to resist a pull on the column to remove it. Below is a list of part numbers, which will allow you to use the new system with all of your columns.
Column ID 0.53 mm 0.32 mm 0.25 / 0.2 / 0.1 mm No hole Back Ferrule (10/pk) 5182-3477 5182-3477 5182-3477 5182-3477 Price $44 44 44 44 Front Ferrule (10/pk) 5182-9673 5182-9676 5182-9677 5182-9679
GC and GC/MS
Note: It is still possible to use the older system of sealing, which will require the purchase of the adapter fitting (P/N G1532-80540), now requiring two sealing ferrules.
Column
TC Detector
Front ferrule
Back ferrule
1/8" Brass nut
186

6890 ECD Supplies/FPD Supplies
6890 Electron Capture Detector (ECD) Supplies
Item 1 2, 3 Description ECD makeup gas adapter* Nut warmer cup with insulation ECD adapter replacement liner, fused silica ECD end cap ECD chemical sample kit, 3 ampoules solution of 33 pg/mL (0.033 ppm) each of lindane and aldrin in isooctane ECD wipe test kit Part No. G1533-80565 19234-60720 19233-20625 19233-20755 18713-60040
18713-60050
*Includes one each of P/N 19233-20625 and P/N 19233-20755
ECD Wipe Test: A wipe test kit (P/N 18713-60050) is supplied with each new ECD. The kit includes an information card with instructions for performing the test. Records of tests and results must be maintained for possible inspection by the NRC (Nuclear Regulatory Commission) and/or responsible state agency.
6890 Series GC ECD Detector
6890 Flame Photometric Detector (FPD) Supplies

Item Description 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 Exit tube assembly Nut, brass, 1/4 in. Ferrule, Vespel/graphite 1/4 in. id Weldment, block O-ring, ignitor, Kalrez Spacer, ignitor Glow plug Ignitor cable assembly Gasket, heat shield Window, first heat shield Disk, heat shield Coupling, SS Lockwasher (4 required) Screw, M3 x 12 (4 required) Clamp O-ring, inner window Window, second heat shield O-ring, outer window Flange adapter Flange ring O-ring, Viton, 1.239 in. id 12/pk 12/pk 12/pk Unit Part No. 19256-60700 5180-4105 5080-8774 19256-80560 0905-1102 19256-20590 0854-0141 G1535-60600 19256-80040 19256-80030 19256-20580 19256-20550 2190-0108 0515-0911 19256-00090 5061-5886 19256-80060 5061-5891 19256-20510 19256-00200 5061-5890
16 17 18 19 20 21 8 7 6 5 4 9 10 11 1 2 3
GC and GC/MS
12 13 14
15
187

5890 Split/Splitless Inlet Supplies
5890 Inlet Supplies

1 2 3 14 13 12 11 2 4
Split/Splitless Multimode Inlet Supplies

Item Description 1 2 3 Septum retainer nut or Retainer nut for headspace sampling Split/splitless insert weldment with tubing Graphite O-ring for split liner Graphite O-ring for splitless liner Fluorocarbon elastomer O-ring for liners 4 5 6 Inlet liners, split, glass wool** Retaining nut SS seal Gold plated seal Gold plated seal, cross 7 8 9 10 Washer Reducing nut Insulation (must order quantity 3) Lower insulation cover 12/pk 12/pk 10/pk 10/pk Unit Part No. 18740-60835 18740-60830 19251-60575 5180-4168 5180-4173 5180-4182 19251-60540 19251-20620 18740-20880 18740-20885 5182-9652 5061-5869 18740-20800 19243-00067 19243-00070
5 6 7 8
10
5890 Split/Splitless Inlet
GC and GC/MS
For Split/Splitless Inlets Using Electronic Pressure Control
11 12 13 14
Splitter tube* Front ferrule, 1/8 in. brass Back ferrule, 1/8 in. brass Tubing nut, 1/8 in. brass 10/pk 10/pk 10/pk 5/pk
19251-80525 5180-4109 5180-4115 5180-4103 19251-00100 5180-4153 8500-4789
Angled wrench for split/splitless inlet Capillary inlet cleaning wires

Capillary Inlet Evaluation Sample (Split Mode):
Gold Plated Seal P/N 18740-20885
Solution of 1.0% of nonane (C9), hexadecane (C16), in tetradecane (C14). Three ampoules.
*Includes 1 each of P/N 5180-4109, 5180-4115, 5180-4103. **For a complete selection of inlet liners, see pages 157158.
Helpful Hint: For optimum gas chromatographic performance, see our complete selection Inlet Septa pages 149153.
188

5890 Split/Splitless Inlet Supplies
Split/Splitless Multimode Inlet Kits
Item Description Split/Splitless Injection Port Kit includes: Gold plated seal Linear, split, with cup, glass wool and packing Liner, split, straight, glass wool, non-deact Ferrule install tool Blanking plug, finger-tight style Washers 0.375 od Liner, splitless, single-taper, glass wool, deact Ferrule, graphite 530 m, 1.0 mm id Ferrule, graphite 320 m, 0.5 mm id Graphite O-ring, 5700/5840/6890 6.52 mm id, 9.63 mm od, 0.62 in. od seals O-rings, ungreased for trap fitting 1/4 in. Viton O-ring Glass wool, pesticide grade Plastic fitting Liner, direct, 2 mm id, deactivated Column nut Capillary tubing cutter Bleed/temp optimized inlet septa 11 mm Tool, wrench Capillary inlet supplies kit includes: Gold plated seal Liner, split, straight, glass wool, non-deact. Liner, splitless, single-taper, glass wool, deact. 1/4 in. Viton O-ring Liner, direct, 2 mm id, deactivated Bleed/Temp optimized inlet septa 11 mm 50/pk 3 each 4 each 2 each 12/pk 10/pk 2 each 2/pk 4/pk 50/pk 2 each 12/pk 3 each 10/pk 10/pk 10/pk 10/pk 12/pk 12/pk 3 each 3 each 3 each Unit Part No. 5181-8838 18740-20885 18740-60840 19251-60540 19251-80680 5020-8294 5061-5869 5062-3587 5080-8773 5080-8853 5180-4168 5180-4173 5180-4181 5180-4182 5181-3317 5181-3394
GC and GC/MS
5181-8818 5181-8830 5181-8836 5183-4757 8710-0510 5182-3449 18740-20885 19251-60540 5062-3587 5180-4182 5181-8818 5183-4757
189

5890 Cool On-Column (COC) Inlet Supplies
Item Description
Manual Injection
Unit
Part No. 19320-80625
1 2 3 4
Cooling tower assembly Duckbill Spring Inserts for capillary columns: For 530 m columns (no rings) For 530 m Al clad columns (4 rings) For 320 m columns (5 rings) For 200 m columns (1 ring) 10/pk
19245-40050 19245-60760 19245-20580 19245-20780 19245-20525 19245-20510
2 3 4
Cool On-Column Inlets for Manual Injection
Fused silica syringe needles Syringe for use with fused silica needles Automatic Injection 1a 1b 2 Septum nut (5890 Series II GC) Needle guide (for 7673A only) Septa Advanced Green 5 mm, through-hole* Septa, BTO 5 mm, solid manual, high pressure* Spring Inserts for capillary columns: For 530 m columns (no rings) For 320 m columns (5 rings) For 250 m columns (6 rings)
6/pk
19091-63000 9301-0658 19245-80520 19245-20670
50/pk 50/pk
5183-4760 5183-4758 19245-60760 19245-20580 19245-20525 19245-20515
GC and GC/MS
3 4
1B 1A 2 3
Common Supplies
5 6
Weldment (refer to instrument manuals) Capillary Column Ferrule** Column nut 2/pk 5080-8853 5181-8833
Column/Retention Gap Installation Consumables
320 m Vespel/graphite ferrule 250 m Vespel/graphite ferrule 320 m retention gap (one 5 m piece) Deactivated quartz column connector, fits 0.18-0.53 mm
10/pk 10/pk 5/pk
5062-3514 5181-3323 160-2325-5 5181-3396
6 7
5890 COC Inlet
*For a complete selection of septa see pages 149153. **For a complete selection of capillary column ferrules see pages 162163.
190

5890 Packed Column Inlet Supplies
5890 Packed Column Inlet Supplies
Item Description Purged Packed Column Inlet Kit: Includes Ferrule Vespel/graphite 1/4 in. for inserts/glass columns 10/pk O-ring, Viton Disposable glass insert 1 Septum retainer nut Headspace septum retainer nut Nonpurging septum nut assembly for manual flow control only, not EPC 2 3 4 5 6 7 8 Septa, BTO 11 mm od Top insert weldment Fluorocarbon elastomer O-rings Weldment (refer to instrument manuals) Vespel ferrules Tubing nut, 1/4 in. brass Adapters without glass liners: 1/8 in. column adapter 1/4 in. column adapter Adapters with glass liners: Agilent Series 530 mm column adapter 1/8 in. column adapter 1/4 in. column adapter Glass liner, disposable, 170 L internal volume Deactivated glass liner, disposable, 170 L internal volume
Universal Packed Column Inlet (non-purged)
Unit
Part No. 5181-8837 5080-8774 5080-8898 5181-3382 18740-60835 18740-60830 19243-60570
12/pk 5/pk
50/pk 12/pk 10/pk 10/pk
5183-4757 19243-80570 5080-8898 5080-8774 5180-4105 19243-80510 19243-80520 19244-80540 19243-80530 19243-80540
Septum-Purged Packed Column Inlet
GC and GC/MS
25/pk 5/pk
5080-8732 5181-3382 19243-60505
1 2 3 4 5 6
Septum retainer nut for headspace sampling, nonpurging Septa, BTO 11 mm od Weldment (refer to instrument manuals) Graphite/Vespel ferrule Brass nut, 1/4 in. Adapters without glass liners: 1/8 in. column adapter 1/4 in. column adapter Adapters with glass liners: Agilent Series 530 mm column adapter 1/8 in. column adapter 1/4 in. column adapter 10/pk 10/pk 50/pk
5183-4757 5080-8774 5180-4105 19243-80510 19243-80520 19244-80540 19243-80530 19243-80540

Universal Packed Column Inlet
191

5890 FID/NPD Supplies
Universal Packed Column Inlet (non-purged) Supplies (continued)
Description Headspace septum nut, non purged inlet, non EPC Deactivated glass liners, disposable, 170 L internal volume 5/pk Glass liner, disposable, 170 L internal volume 25/pk Unit Part No. 19243-60500 5181-3382 5080-8732
5890 Detector Supplies

Flame Ionization and Nitrogen Phosphorus Detector Supplies
2 3 4 5 6
Item Description
FID Supplies
Unit
Part No.
1 2 3 4 5 6 7
Collector assembly (contains items 2-9) PTFE chimney (optional) Collector nut Spring washer Ignitor castle Optional Hastelloy ignitor castle Ignitor (glow plug) assembly Upper collector insulator Collector body Optional hastelloy collector Lower collector insulator Detector nut warmer (not shown) FID performance evaluation sample kit: Solution of 0.03% C14, C15, and C16 in hexane, 3 ampoules, 0.5 mL volume FID supplies kit-Includes: Jet, packed standard 0.018 in. id tip FID performance evaluation sample kit Ignitor glow plug assembly Jet, capillary series 530 m, 0.011 in. id tip FID flow measuring insert Cleaning wires for 0.03 in. id jet Cleaning wires: 0.018 in. id/530 m jet Wire, jet cleaning Capillary inlet cleaning wires Detector clean kit 3 each 2 each 2 each 3 each 2 each 5/pk 5/pk 5 each 5/pk 10/pk
19231-60690 19231-21050 19231-20940 5181-3311 19231-20910 19231-21060 19231-60680 19231-20970 19231-20960 19231-21080 19231-20950 19234-60720 18710-60170
8 9
GC and GC/MS
5890 FID Supplies
8 9
5182-3450 18710-20119 18710-60170 19231-60680 19244-80560 19301-60660 5180-4150 5180-4152 19301-20720 5180-4153 9301-0985
Helpful Hint: Hastelloy components should be used when analyzing highly corrosive materials such
as chlorinated solvents for HCl via flame.
192

5890 FID/NPD Supplies
Flame Ionization and Nitrogen Phosphorus Detector Supplies (continued)
Item Description FID flow measuring insert FID collector cleaning brush 1/4 in. nut driver for 5890A FID jet, drilled shaft
Jets:
Unit 2/pk
Part No. 19301-60660 8710-1346 8710-1561
Capillary/Series 530 mm jet (0.011 in. id tip) Packed standard 0.018 in. id jet Packed wide-bore 0.030 in. id jet (for high-bleed applications) High-temperature jet (for simulated distillation) FID flow measuring adapter O-ring for flow adapter Jet cleaning wire for 0.03 in. id jet Jet cleaning wire for Series 530 mm and 0.018 in. id jet
NPD Supplies
19244-80560 18710-20119 18789-80070 19244-80620 19301-60660 5080-4978 5/pk 5/pk 5180-4150 5180-4152
NPD collector (NPD bead) Recoating kit, sufficient for 10 collectors NPD sample: solution of 0.65 ppm azobenzene, 1,000 ppm octadecane, and 1.00 ppm malathion in isooctane, 3 ampoules, 0.5 mL volume
FID and NPD Adapters
19234-60540 5080-8872 18789-60060
Adapters
5 6 2
GC and GC/MS
1 2 3 4 5
FID/NPD 1/4 in. packed column FID/NPD 1/8 in. packed column FID/NPD capillary column FID/NPD capillary column nut FID/NPD Vespel ferrule, 1/4 in. FID/NPD graphite ferrule, 1.0 mm (not shown) Graphite ferrule, 0.5 mm (not shown) 2/pk 10/pk 10/pk 10/pk 10/pk
19231-80530 19231-80520 19244-80610 5181-8830 5080-8774 5080-8773 5080-8853 5180-4105
1/8 in. Packed Column Use 5 6 1
1/4 in. Packed Column Use 5 6 3 4
Tubing nut, 1/4 in. brass
Capillary Column Use
193

5890 TCD/ECD Supplies
7
3 4
Thermal Conductivity Detector TCD Supplies

Item Description Unit Part No.
TCD
1
2 8 1
Capillary column nut Graphite ferrule, 0.5 mm id Graphite ferrule, 1.0 mm id Vespel ferrule, 1/8 in. id Tubing nut, 1/8 in. brass Makeup gas adapter, TCD TCD column adapter for capillary columns TCD column adapter seal TCD column adapter, 1/8 to 1/4 in. glass
2/pk 10/pk 10/pk 10/pk 10/pk
5181-8830 5080-8853 5080-8773 0100-1332 5180-4103 19232-80550 18740-20960 18740-20950 19302-80020 19232-60676 18711-60060
2 3
TCD Capillary Adapter without Make up
TCD for Packed or 1/4 in. Glass Column
4 5 6 7 8
TCD replacement cartridge TCD performance evaluation sample: C14, C15, and C16 (0.3%) in hexane, 3 ampoules, 0.5 mL volume
TCD Adapter with Make up
Electron Capture Detector ECD Supplies

Item Description Unit 10/pk 10/pk Part No. 5080-8774 0100-1331 5180-4105 19301-80530 19233-80565 G2397-80520 19233-20625 G2397-20540 19233-20755 18713-60040 1 2 Vespel graphite ferrule, 1/4 in. id Vespel/graphite 85%/15% ferrule, 1/4 in. id Tubing nut, 1/4 in. brass ECD column adapter, 1/4 to 1/8 in. metal Makeup gas adapter, ECD, new style Makeup gas adapter for micro ECD adapter ECD fused silica liner (new style) ECD adapter fused silica liner micro ECD Stainless steel cap for ECD makeup gas adapter (new style and micro ECD)
GC and GC/MS
12 11
1 2
3 5 10 11 12
5, 10
ECD Make up Gas Adapter
ECD Packed for 1/4 in. Glass Columns
ECD Performance evaluation sample lindane and aldrin (0.033 ppm) in Isooctane 3 ampoules, 0.5 mL
Helpful Hint: To increase your GC performance, see our complete line of gas specific or contaminant specific purifiers.
194

5890 FPD Supplies
Flame Photometric Detector FPD Supplies
Description Adapter weldment, 1/8 in. columns Adapter weldment, capillary Start-up kit Exit tube Window O-ring, inner Window O-ring, outer Window, heat shield, 1st Window, heat shield, 2nd Ferrule, 1/4 in., Vespel Glow plug FPD O-ring O-ring, Viton Liner/ferrule kit Sulfur filter Phosphorus filter O-ring, greased Kalrez O-ring, size 2-002 Kalrez O-ring, size 2-010 Kalrez O-ring, size 2-011 Fluorocarbon Elastomer O-ring, brown, 0.926 in. id Fluorocarbon Elastomer O-ring, brown, 1.239 in. id FPD Performance Evaluation Sample: Solution of 20 ng/mL (20 ppm) dodecanethiol and tributylphosphate in isooctane, 3 ampoules 12/pk 12/pk 12/pk 12/pk 12/pk 10/pk 12/pk 12/pk Unit Part No. 19256-80590 19256-80570 19256-60500 19256-60700 5061-5886 5061-5891 19256-80030 19256-80060 5080-8774 0854-0141 5061-5867 5180-4182 19256-60590 19256-80000 19256-80010 5080-8846
GC and GC/MS
0905-1101 0905-1102 0905-1103 5061-5889 5061-5890 19305-60580
195

AED Supplies
G2350 Atomic Emission Detector Supplies

The G2350A Atomic Emission Detector Brings Speed and Selectivity
Environmental laboratories are using the Atomic Emission Detector (AED) to make GC analysis of unknowns faster and more accurate. The AED quickly and efficiently screens for a wide range of substances without losing unknown compounds. At picogram levels, the AED selectively detects contaminants containing Cl, N, S, and P without interference from elements such as phthlates and ketones. The Agilent AEDs are also able to detect organometallic compounds containing Hg, Fe, Sn, As, Ni, V, Se, and Si. Atomic emission detection makes routine work out of the most difficult analyses.
5921 Atomic Emission Detector
G2350 AED Supplies

Description Lens keeper O-ring, Viton Gas Union 1/16 in. tubing nut, SS Discharge Tube Unit 12/pk 10/pk Part No. 5061-5889 5180-4102 G2350-61090
GC and GC/MS
5921/G2350A AED Supplies

Description Window O-ring Spectrometer window replacement kit Gas union brazement Purge manifold assembly 0.53 mm ferrules, brass 0.32 mm ferrules, brass 0.20 mm ferrules Plug ferrules 1/16 in. to 0.5 mm Vespel Graphite reducing ferrule 1/16 in. to 0.4 mm Vespel Graphite reducing ferrule Ferrule nut Ferrule key Discharge Tube 10/pk 10/pk 10/pk 10/pk 10/pk 10/pk Unit Part No. 0905-1161 05921-80350 05921-80600 05921-61390 5021-7134 5021-7137 5021-7136 5021-7133 5062-3506 5062-3508 05921-21850 05921-21860 05921-61090
196

AED Supplies
Agilent 5921/G2350A Common Supplies
Description GC chamber O-ring Optical chamber O-ring Receiver liner O-ring (large) Receiver liner fitting nut Receiver liner ferrules Receiver liner O-ring (small) 1/8 in. Teflon ferrules 1/4 in. Vespel ferrules 1/16 in. Vespel ferrules Nut, 1/16 in. SS Transfer line 0.5 mm graphite ferrule Fly ash filter 1/4 in. filter, 5 m water filter element (internal water system only) 1/4 in. filter, 60 m water filter element (external water system only) Cavity brush, small Cavity brush, large AED performance sample Sample 1: Contains (w/w) 0.07% nitrobenzene, 0.43% n-tridecane, 0.07% 4-fluoroanisole, 0.05% tert-butyldisulfide, 0.08% 1,2, 4-trichlorobenzene, 0.05% tetraethyl orthosilicate, 0.05% n-decane (perdeuterated), 4.3% n-dodecane, 0.13% n-tetradecane, 0.07% 1-bromohexane, 4.04% n-octane in isooctane. Sample 2: Contains the same components as Sample 1 plus 0.06% triethylphosphate. Six 0.5 mL ampoules. Three of each sample. AED check-out column (HP-1) 25 m, 0.32 mm, 0.17 m 19091Z-012 10/pk 10/pk 10/pk 10/pk 10/pk Unit Part No. 0905-1159 0905-1160 0905-1163 0100-1505 0100-1504 0905-1162 0100-1365 0100-1327 0100-1329 5180-4102 5080-8853 3150-0417 3150-0617 3150-0374 8710-1876 8710-1938 8500-5067
GC and GC/MS
197

6890/5890 Regulators, Mass Flow Controllers, and Restrictors
Backpressure Regulators
Description 0-15 psig backpressure regulator/gauge kit 0-60 psig backpressure regulator/gauge kit 0-15 psig (0-100 kPa) 0-30 psig (0-200 kPa) 0-60 psig (0-400 kPa)
Backpressure Regulator
Part No. 19246-60620 19246-60630 19246-60560 19246-60570 19246-60580 19246-60690
0-100 psig (0-700 kPa)
Pressure Gauges
Description 0-15 psig (0-100 kPa) 0-30 psig (0-200 kPa) 0-60 psig (0-400 kPa) 0-100 psig (0-700 kPa) Part No. 19246-60526 19320-60656 19363-60506 19361-60566
Mass Flow Controllers

Description Part No. 19362-60565 19362-60575 Mass flow controller with 20 mL/min restrictor for use with packed column inlets Mass flow controller with 400 mL/min restrictor for use with capillary inlets
GC and GC/MS
Restrictor-New Style
Description 0-20 mL/min 0-60 mL/min 0-110 mL/min
Mass Flow Controller Restrictor (old style screw type)
Color Dot Code Red and Purple Blue and Purple Green and Purple Black and Purple Yellow and Purple
Part No. 19362-60515 19362-60525 19362-60535 19362-60545 19362-60555
0-400 mL/min 0-750 mL/min
Restrictor-Old Style
Description 0-20 mL/min 0-60 mL/min 0-110 mL/min 0-400 mL/min 0-750 mL/min Color Code Red Red Green Black Silver Green Color Dot Silver Part No. 19362-60510 19362-60520 19362-60530 19362-60540 19362-60550
198
GC/MS Parts and Supplies

MSD Maintenance Schedule
Maintaining Mass Selective Detectors (MSD)

Your GC/MSD system contains a high level of sensitivity. To continue achieving optimal results, it is critical to maintain your system properly by performing the essential tasks within this section. Some of the benefits for maintaining your GC/MSD include: less downtime for repairs a longer lifetime for your MSD system a reduction in overall operating costs
Maintenance Schedule
Some parts of the MSD require regularly scheduled maintenance. Common maintenance tasks are listed below. It is advisable to keep a log book of system performance, Autotune, and maintenance operations performed. This makes it easier to identify variations from normal performance and to take corrective action.
Every Day
Check, and if necessary, replace the septum. Check the injection port liners. Check the tightness of the column nuts.
Every Week
Check the foreline pump oil level and diffusion pump fluid. Change the injection port liners and O-rings. Gas ballast the foreline pump.
GC and GC/MS
Every Month
Clean the split/splitless inlet vent line trap. Check for leaks (inlet and column connections).
Every Three Months

Replace gas cylinders (when below 500 psig).
Every Six Months

Replace the foreline pump oil. Check, and if necessary, refill the calibration vial.
199

MSD Maintenance Schedule Every Year
Replace the diffusion pump fluid. Recondition or replace internal and external traps and chemical filters on the GC.
As Needed
Tune the MSD. Clean the ion source. Replace the carrier gas trap. Replace worn-out parts (filaments, EM, etc.). Replace the column. Lubricate seals.
Monitor
Record all tune values such as electron multiplier and ion source parameters in a log book to monitor instrument performance. In addition, note the high vacuum and foreline vacuum pressures.
Every week Every 6 months Every year As needed
Task Tune the MSD Change injection port liners Check the foreline pump oil level Gas ballast the foreline pump Check the calibration vial Replace the foreline pump oil Check the diffusion pump fluid Replace the diffusion pump fluid Replace the traps and filters Clean the ion source Change the carrier gas trap(s) and purifier Replace the worn out parts Lubricate seals (where appropriate) Replace column
GC and GC/MS
200

MSD Contamination
Contamination
Contamination is usually identified by excessive background in the mass spectra. It can come from the GC or from the MSD. The source of the contamination can sometimes be determined by identifying the contaminants. Some contaminants are much more likely to originate in the GC, others are likely to originate in the MSD. Contamination sources originating in the MSD: Air leaks Cleaning solvents and materials Fingerprints inside the manifold Diffusion pump fluid Foreline pump oil
Air Leaks
Air leaks are a problem for any instrument that requires a vacuum to operate. Leaks are generally caused by vacuum seals that are damaged or not fastened correctly. Symptoms of leaks include: Higher than normal vacuum manifold pressure or foreline pressure Higher than normal background Peaks characteristic of air (m/z 18, 28, 32, and 44 or m/z 14 and 16) Poor sensitivity Low relative abundance of m/z 502 (this varies with the tune program and MSD used) Remedy Check interface nut for tightness. Replace if necessary. Check, and leak test the GC injection port. Helpful Hint: The most likely point for an air leak is a seal you recently opened.
Description MS interface column nut Column nut for GC/MS and two hole ferrules Universal column nut 2/pk Unit Part No. 05988-20066 05921-21170 5181-8830
GC and GC/MS
201

MSD Contamination Cleaning Solvents
It is common to see cleaning solvent peaks in the mass spectra shortly after the ion source is cleaned. Remedy Dry all cleaned metal parts in the GC oven before reassembling and reinstalling them. Refer to specific cleaning procedures in your MSD Hardware Manual. Use a temperature above the boiling point of the solvent but below the limit of the column.
Fingerprints
Fingerprints contain hydrocarbons that can appear in mass spectra. Hydrocarbon contamination is characterized by a series of mass peaks 14 amu apart. The abundances of these peaks decrease as peak mass increases. Fingerprint contamination is usually caused by the failure to wear lint-free, nylon gloves during ion source cleaning, GC inlet maintenance, or from installing the column. Use special care to avoid recontamination of parts after you clean them. This typically occurs after some maintenance or part replacement. Remedy Reclean using clean, nylon gloves and proper cleaning techniques.
Description Nylon gloves, lint-free, large Nylon gloves, lint-free, small Part No. 8650-0030 8650-0029
GC and GC/MS
Diffusion Pump Fluid

If the diffusion pump is allowed to operate with no column (carrier gas) flow into the vacuum system, vapor from the diffusion pump fluid can drift up into the vacuum manifold. A more serious problem is when fluid is back streamed into the vacuum manifold by sudden or improper venting of the vacuum system. If a diffusion pump has back streamed, a prominent peak will often be seen at m/z 446 and the spectral baseline will exhibit increased background noise. Remedy If m/z 446 appears please call Agilent for assistance.
Foreline Pump Oil

Foreline pump oil contamination is characterized by peaks spaced 14 amu apart (hydrocarbons). Contamination with foreline pump oil is less common than contamination with diffusion pump fluid. Remedy Call Agilent for assistance.
202

MSD Contamination Contamination Identification
The following table lists some of the more common contaminants, the ion characteristic of those contaminants, and the likely sources of those contaminants.
Ions (m/z) 13, 14, 15, 16 18, 28, 32, 44 or 14, 16 31, 51, 69, 100, 119, 131, 169, 181, 214, 219, 264, 376, 414, 426, 464, 502, 576, 614 31 43, 58 78 91, 92 105, 106 151, 153 69 73, 147, 207, 221, 281, 295, 355, 429 77, 94, 115, 141, 168, 170, 262, 354, 446 149 Compound Methane H2O, N2 , O2 , CO2, CO2 or N, O PFTBA and related ions Possible Source CI gas Residual air and water, air leaks, outgassing from Vespel ferrules PFTBA (tuning compound)
Methanol Acetone Benzene Toluene or xylene Xylene Trichloroethane Foreline pump fluid or PFTBA Dimethylpolysiloxane Diffusion pump fluid Plasticizer (phthalates)
Cleaning solvent Cleaning solvent Cleaning solvent Cleaning solvent Cleaning solvent Cleaning solvent Foreline pump oil vapor or calibration valve leak
GC and GC/MS
Septum bleed or methyl silicone column coating Diffusion pump fluid and related ions Vacuum seals (O-rings) damaged by high temperatures, use of vinyl or plastic gloves Fingerprints, foreline pump oil
Peaks spaced 14 amu apart
Hydrocarbons
203

MSD Ion Source
Maintaining the Ion Source

Cleaning procedures for MSDs vary. Refer to your MSD Hardware Manual for specific ion source cleaning procedures. Common measures of instrument performance: Abundance of certain ions (e.g., percentage of the 502 ion from the Autotune report) Shape of lens ramps and the chosen voltages, especially Repeller Ramp Sensitivity obtainable for a given analysis Ability to tune to a given reference compound (e.g., DFTPP) When to clean: The ion source should be cleaned: According to a customers predefined schedule Based on instrument performance (e.g., deteriorated performance over time)
5973 Ion Source Assembly (EI)
Frequency of cleaning: The number of samples run (throughput) The type of samples Unique, established laboratory protocol Selecting a cleaning method: The primary action of any cleaning procedure is to remove contamination from surfaces. Removing this contamination restores the electrostatic properties of the ion source lensing system. Numerous cleaning methods have been developed for restoring ion source performance. The cleaning methods include abrasive, sonic, and electropolish. Abrasive methods offer several advantages: Provide adequate energy to remove contamination from surfaces Require minimal equipment Pose minimal risks to the user
GC and GC/MS
204

MSD Ion Source
Cleaning and Maintenance Supplies
Description
Cleaning and Maintenance
Part No. 8650-0030 8650-0029 9310-4828 5181-8863

Cleaning and Maintenance Supplies
Nylon gloves, lint-free, Large Nylon gloves, lint-free, Small Lint-free industrial wipes, 100% cotton, 9 x 9 in. (300/pk) Generic ion source cleaning kit for all GC/MS types Includes: Cloths, lint-free (15/pk), Abrasive sheets (5/pk), Cotton swabs (100/pk), Nylon gloves, lint-free, Alumina powder, abrasive Cloths, lint-free (15/pk) Abrasive Sheets, aluminum oxide green lapping paper for ion source cleaning, 600 mesh (5 sheets) Alumina powder, abrasive PFTBA sample, certified (10 g) PFTBA sample kit, 0.5 mL Activated alumina, absorbent pellets for Edwards rough pump traps, non-LC/MS (1 lb can) PFTBA glass vial Cotton swabs (100/pk)
Tools
05980-60051 5061-5896 8660-0791 8500-0656 05971-60571 8500-1233 05980-20018 5080-5400
GC and GC/MS
Screwdriver, Pozidriv #1 pt, 3 in., fits no. 2 - 4 screws Screwdriver, Pozidriv #2 pt, 4 in., fits no. 5 - 10 screws Wrench, open-end, 1/4 x 5/16 in. Hex nut driver, 5.5 mm Screwdriver, TORX, T20 Screwdriver, TORX, T15 Screwdriver, TORX, T10 Ferrules and O-rings Teflon 1/4 in. ferrule (back) Teflon 1/4 in. ferrule (front) Retainer rings (10/pk)
8710-0899 8710-0900 8710-0510 8710-1220 8710-1615 8710-1622 5182-3466 0100-0160 0100-0787 5181-1258
205

MSD Ion Source
Cleaning and Maintenance Supplies (continued)
Description 5973 One Year Maintenance Kit (for diffusion pump systems) Includes: Big Universal Trap for He (1/8 in.), Abrasive sheets (5/pk), Cloths, lint-free (15/pk), Cotton swabs (100/pk), SantoVac Ultra, 18.5 mL (2 each), Rough pump oil, 1 liter, Filament assembly, Octafluoronapthalene (OFN) MSD Tool Kit Includes: Small cleaning rod, Large cleaning rod, Source hold tool, Cotton swabs (100/pk), Nylon gloves, lint-free, Abrasive sheet, 30 mm (5/pk), Tool kit (wrenches, driving tools) Part No. 5183-2096
05971-60561
Helpful Hint: Details about specific MSD maintenance can be found in your models specific hardware manual.
MSD Ion Source Parts (EI) 5973

Item (A) (B) (C) Description Ion source body Repeller Interface socket Drawout plate Drawout cylinder Ion focus lens Entrance lens Repeller insulator Set screw Washer, M3 (5/pk) Nut, 5.5 mm Ion Source Sensor Lens insulator, (2/pk) Ion source assembly Source heater assembly Repeller assembly Screw (for filament on the source) 5973 Part No. G1099-20130 G1099-20132 G1099-20136 05971-20134 G1072-20008 05971-20143 05971-20126 G1099-20133 0515-1446 3050-0891 0535-0071 G1099-60104 05971-20130 G1099-60106 G1099-60177 G1099-60170 0515-1046
GC and GC/MS
C H M L
(D) (E) (F) (G) (H) (I) (J) (K) (L) (M)
F E D
5973 Ion Source Parts (EI)
B I I H K J L H
5973 Repeller Assembly (EI)
206

MSD Ion Source/MSD Filaments
5972/5971/GCD MSD Ion Source Parts (EI)
Item (A) (B) (C) (D) (E) (F) (G) (H) (I) Description Entrance lens Lens insulator Ion focus lens Drawout cylinder Drawout plate Ion source body Set screw Repeller assembly Screw (for filament on the source) Ion source assembly (5972) Ion source assembly (5971/GCD) Transfer line tip, gold plated (5972/5971) Transfer line tip, gold plated (GCD) 2/pk Unit Part No. 05971-20126 05971-20130 05971-20143 G1072-20008 05971-20134 05971-20128 0515-1446 05971-60170 0515-1046 05971-60226 05971-60102 05971-20305 G1800-20305
5972/5971/GCD Ion Source (EI)
B A D F C B E G H
Helpful Hint: It is good practice to replace scratched lenses and other ion source parts. Scratched source parts lead to poor performance.
GC and GC/MS
Maintaining the Filaments

Like the filament in an incandescent light bulb, the ion source filaments will eventually burn out. Certain practices will reduce the chance of early failure: When setting up data acquisition parameters, set the solvent delay so that the analyzer will not turn on while the solvent peak is eluting. When the software prompts Override solvent delay at the beginning of a run always select No. Higher emission current will reduce filament life. If you are controlling your MSD from the Edit Parameters screen, always select MS Off before changing any of the filament parameters. Description Filament, 5973 (EI) Filament, 5973 (CI) Filament, 5972 (EI/CI) Filament, 5971 (EI/CI) / GCD (EI) 2/pk Unit Part No. 05971-60053 G1099-80053 05971-60053 05971-60140
5973/5972 Filament
Helpful Hint: It is very useful to switch from one filament to the other every three months so that when one filament fails, you know the other will fail soon. This will allow you to change both filaments at the same time.
207

MSD Vacuum Systems and Pumps
Vacuum Systems and Pumps

A properly maintained vacuum system will: Prevent premature filament failure Provide better sensitivity Require less frequent source cleaning Extend quadrupole lifetime Prevent premature EM Horn failure Helpful Hint: Keeping a pan under the vacuum pump helps to detect and identify the origin of oil leaks.
Calibration
The calibration valve is an electromechanical valve with a vial for tuning compounds. Perfluorotributylamine (PFTBA) is the most commonly used tuning compound. It is required for automatic tuning of the MSD in EI mode. The tuning compound is usually a liquid but can be a volatile or semi-volatile solid.
Description PFTBA sample, certified PFDTD sample Unit 10 g 10 g Part No. 8500-0656 8500-8130
Note: If using the 5973 with a CI source, use Perfluorodimethyltrioxadodecane (PFDTD) sample.
GC and GC/MS
Diffusion Pump
It is not necessary to change the diffusion pump fluid more than once a year, unless you observe symptoms that suggest a problem with the diffusion pump fluid. The MSD must be vented in order to check the diffusion pump fluid (except for the 5973). Therefore, the best time to check the fluid is when the instrument is already vented for other maintenance. How to Check the Fluid Level: 1. If it is not vented already, shut down and vent the MSD according to instrument manual. 2. Unplug the MSD power cord. 3. Remove the pump and cover the top with aluminum foil. 4. After heating the pump in a GC oven at 60C for 15 minutes to make the fluid flow down into the reservoir at the bottom, remove the stack parts. 5. Inspect the pump fluid. If the fluid is discolored or contains particulate material, the fluid must be changed. 6a. Use a metal ruler to determine the depth of the fluid. A pump that has been in operation should have a pool 9 mm +/- 1 mm deep. Fluid in freshly charged pumps will be 12 mm deep. It is normal that up to 2 mL of oil may be in the rear portion of the vacuum manifold. The recommended total fluid charge for the 5971/5972 is 18 mL (+/- 2 mL). 6b. For the 5973 use the sight glass to determine the depth of the fluid. The recommended total fluid charge is approximately 37 mL. Helpful Hint: Use chemical-resistant gloves and safety glasses when replacing pump fluid. Avoid all contact with the fluid.
208

MSD Vacuum Systems and Pumps
Description Diffusion pump fluid: SantoVac Ultra 5P, 18.5 mL (5973, 5972 or 5971/GCD) Ion gauge controller (5973/5972A) Ion gauge tube for measuring vacuum (5971/5972) Triode gauge tube for measuring vacuum (5972/5973) Part No. 6040-0809 59864B 0960-0376 0960-0897
Foreline Pump
The oil in the foreline or rough pump should be replaced on average once every six months, but can vary depending upon applications. After oil replacement, if a foreline trap is present, the molecular sieves should be replaced. Avoid contact with the pump oil. The residue from some samples may be toxic. Dispose of used oil properly.
Description Rough pump oil, 1 gal Inland 45 (5973, 5972 or 5971/GCD) Rough pump oil, 1 liter Inland 45 (5973, 5972 or 5971/GCD) Molecular sieve (5973, 5972 or 5971/GCD) Part No. 6040-0798 6040-0834 9301-1104
General Instructions on How to Replace the Pump Oil
GC and GC/MS
1. Vent and shut down the MSD. 2. Place a container under the drain plug on the foreline pump. 3. Remove the fill cap from the top of the pump to expose the fill hole. 4. Remove the drain plug from the pump. 5. Reconnect the MSD to its power source. Switch on for 2 or 3 seconds, and then switch it off again. This displaces old oil from the internal pump cavities. Disconnect the power cord again. 6. Reinstall the drain plug and pour pump oil into the fill hole. 7. Reinstall the fill cap. 8. Reconnect the MSD power cord. 9. Start up and pump down the MSD according to the Instrument Manual procedure. Note: Subtle differences may exist between MSD models. Consult your hardware manual for specific instruction.
209

Electron Multipliers and Replacement Horn
Electron Multipliers and Replacement Horn

Maximize Lifetime
The lifetime of an EM is directly related to the current that flows through it and the extent of contamination or condensation that it experiences. To maximize electron multiplier life: Maintain the best possible vacuum, especially in the analyzer manifold. Use extreme caution and be conservative with venting, pumpdown, and all vacuum system procedures to keep pump fluid background to a minimum. After venting, allow four hours for pump-down and thermal equilibration before scanning. Actively look for background contamination and leaks and repair them immediately. Dont tune excessively. PFTBA can result in higher background over an extended period of time. Symptom Voltage is over 2500 volts Poor vacuum Remedy Replace electron multiplier or horn
Description Electron multiplier replacement horn (5973, 5972, 5971, GCD) High energy dynode (5973 only) Part No. 05971-80103 G1099-80001 05971-80102
Electron Multiplier Replacement Horn
GC and GC/MS
Electron multiplier kit (5971 & 5972 only)
Note: These are the recommended replacement multipliers and horns for the MSD. Substituting other manufacturers products can result in reduced sensitivity, lifetime, and noise problems.
210

MSD NoVents
MSD NoVents
A Timesaving Solution
Agilent Technologies' MSD NoVents allow users to turn on the purge gas and change the column or service the inlet or column without venting. No air or water enters the system. The MSD remains stable and is operational in minutes. Advantages of Agilent's MSD NoVents No oxygen or air in your system during inlet maintenance, prolonging column life A constantly stable MSD, saving hours with each column change or inlet maintenance No MSD or interface temperature cycling, minimizing the possibility of leaks A cleaner ion source, with less downtime for servicing Convenient accessibility in the GC oven Proven fitting design for easy column removal or installation Simple operation, perfect for inexperienced users MSD NoVents are available in two different kits that include all the necessary tools, fittings and instructions: For Agilent 6890/5973/5972 GC/MSD. Designed for seamless integration with the Agilent 6890 GC system. Available exclusively from Agilent. For Agilent 5890/5972/5971 and GCD. Both MSD NoVent kits include a helium purge gas control module that, for the Agilent 6890 MSD NoVent, fits into one of the GC detector slots and is controlled by the Agilent 6890 electronics. For the Agilent 5890 MSD NoVent, the module sits externally and is controlled manually. Both setups require a specific restrictor tube that acts as the transfer line from the GC to the MS source.
GC and GC/MS
MSD NoVent Tee
The MSD NoVent uses a unique fluidic valve design and deactivated fused silica interface tube that mounts directly on the MS interface inside the GC oven. A soft brass threaded insert connects to the MS interface, providing no interface damage. This design ensures that a helium purge maintains a positive flow from the valve to the atmosphere when a column is disconnected, preventing air or water from entering the MS.
211

MSD NoVents/8270 SemiVolatiles Applications Kit
MSD NoVents
Description 6890 MSD NoVent Includes two each restrictors for 5973 and 5972.
6890 MSD NoVent
Part No. 5183-4782 5183-4783
5890 MSD NoVent Restrictors not included and must be ordered separately.
MSD NoVent Replacement Parts

Description MSD NoVent Restrictors ID (mm) Unit Part No.
For use with 5971/5972 For use with 5973 For use with GCD
SilTite Metal Ferrules
2/pk 2/pk 2/pk
5183-4785 5183-4786 5183-4787
For use with 0.20-0.25 mm id capillary columns. Includes 2 column nuts For use with 0.32 mm id capillary columns. Includes 2 column nuts For use with 1/16 in. od SS tubing. Includes 2 column nuts Interface Tee
0.4 0.5
10/pk 10/pk 10/pk
5184-3569 5184-3570 5184-3571 5185-5800
GC and GC/MS
G2860A 8270 Semi-Volatiles Applications Kit

The G2860A 8270 Semi-Volatiles Applications Kit is designed for use in Agilent 6890/5973A and 6890/5973N GC/MSD Systems. The kit provides modified and/or pretested components to improve system performance for USEPA Method 8270. With the kit, system linearity is maximized and activity is minimized. Contents: Start-Up Guide, pub. no. 5988-3073EN Application Note, Improvements in the Agilent 6890/5973 GC/MSD System for use with USEPA Method 8270, pub. no. 5988-3072EN Ultra Ion Source Chamber Ultra Repeller Ultra Large Aperture Drawout Plate Pre-tested column, 30 m x 250 m x 0.5 m HP5 MS, P/N 19091S-139 Single-taper splitless liner, 4 mm id, deactivated, P/N 5181-3316 Direct Connect Liner, single taper, 4 mm id, deactivated, P/N G1544-80730 Direct Connect Liner, dual taper, 4 mm id, deactivated, P/N G1544-80700 Floppy Disk with tuning macros
Description 8270 Semi-volatiles Application Kit Part No. G2860A
212

MS Engine Supplies
Maintaining the MS Engine

Common maintenance tasks are listed in the following maintenance schedule table. Performing these tasks on a regular basis can reduce overall operating costs. Keep a record (logbook) of system performance characteristics and maintenance operations performed. This makes it easier to detect variances from normal operation and to take corrective action.
Task Autotune or manual tune (save results)
Cabinet Maintenance
Every Week
Every 3 Months
Every 6 Months
As Needed

Clean the cabinet Inspect hoses and cords Vacuum the fan filter
Vacuum System Maintenance

Check mech. pump oil Replace mech. pump oil Replace mech. pump traps Check diffusion pump fluid Degas ion gauge tube Replace ion gauge tube Replace seals & O-rings
Analyzer Maintenance

GC and GC/MS
Clean ion source Replace filament Replace ion source heater Replace mass filter heater Replace electron multiplier horn
GC/MS Interface Maintenance

Refill EI calibration vial Refill CI calibration vial Replace interface heater

Helpful Hint: We recommend keeping a separate set of tools that have been thoroughly cleaned for working with the MS engine. These tools are needed to prevent contamination when reassembling or installing clean assemblies such as the ion source.
213

MS Engine Supplies Cleaning the Ion Source
There is not a regular interval for ion source cleaning. The ion source should be cleaned when symptoms indicate. Symptoms of a dirty ion source include poor sensitivity and inadequate abundances at high masses. See the Troubleshooting chapter in your hardware manual for more information about these symptoms. Many of the tools and supplies needed to service the 5989 MS Engine are included in the installation kit supplied with the instrument. The following table lists common cleaning supplies and consumable parts used in the maintenance of the MS Engine.
Cleaning and Maintenance

Description Abrasive paper, 30 mm Alumina powder, abrasive Cloths, lint-free Lint-free industrial wipes, 100% cotton, 9 x 9 in. Cotton swabs Nylon gloves, lint-free Grease, high-vacuum PFTBA sample, certified PFTBA sample kit 15/pk 300/pk 100/pk 1 pair 10 g 0.5 mL Unit 5/pk Part No. 5061-5896 8660-0791 05980-60051 9310-4828 5080-5400 8650-0030 6040-0289 8500-0656 05971-60571
GC and GC/MS
5989 MS EngineIon Source Parts and Supplies

Item (A) (B)
5989 Lens Stack
Description Clamp insulator, ion source Entrance lens Lens insulator, ion source Plate insulator, ion source EI/CI repeller Repeller insulator Filament block Filament assembly Heater cartridge
Part No. 05989-20110 05989-67002 05989-20111 05989-20109 05989-20145 05989-20119 05989-20165 05985-60179 05989-60098
(C) (D) (E) (F)
5989 Repeller Assembly
214

MS Engine Supplies
MS Engine Pump Lubricants and Oils
Lubricant Oil Turbo pump lubricant: 5989 or 5988 Thermo or Electro Spray 5985 or 5987 5999x, 5993x or 5995x Part No.
Balzers turbo pump lubricant, 0.25 liter

Diffusion pump oil:
6040-0468
SantoVac 5, 1 liter SantoVac 5, 128 mL

Rough pump oil:

6040-0370 6040-0819
Rough pump oil, 1 gal Inland 45 Rough pump oil, 1 liter Inland 45 Particle Beam pump oil, Fomblin

6040-0798 6040-0834 6040-0730
Replacement Parts and Supplies

The electron multiplier lifetime on the 5989 may be 6 to 9 months or shorter, especially with the following applications: Thermospray Particle Beam Purge and trap, with no jet separator Extensive CI High sensitivity work with high EM voltage
Description
5989
GC and GC/MS
Unit
Part No. 05989-80043 05971-80103
Electron multiplier kit Electron multiplier replacement horn

Parts and Supplies
Ferrule Ion gauge tube, K-25 flange

O-rings and Seals
2/pk
5181-3366 0960-0799
Insulating ring O-ring, detector flange Diffusion pump fluid fitting fill and drain cap O-ring KF 25 centering ring and O-ring O-ring, manifold window 5/pk 12/pk
05989-20705 5181-3367 0905-1145 3162-0110 0905-1189
215

MS Test and Performance Samples
MS Test and Performance Samples

Each GC/MS has a specific test and performance sample. Refer to the chart below for the exact sample.
Tuning Samples Performance Verification Samples El Cl MSD Tune Tune 5973 PFTBA PFDTD 5972 PFTBA PFTBA 5971 PFTBA PFTBA GCD PFTBA NA El OFN 1pg/L HCB 10 pg/L HCB 10 pg/L Sample A 10 ng/L Negative Mode Cl OFN 1pg/L NA NA NA Positive Mode Cl Checkout Samples SemiVolatile Volatile BFB BFB BFB BFB
Benzophenone DFTPP 100 pg/L Benzophenone DFTPP 100 pg/L Benzophenone DFTPP 100 pg/L NA DFTPP
MS Engine
5989A PFTBA PFTBA 5989B PFTBA PFTBA
HCB 50 pg/L HCB 20 pg/L
OFN 1 pg/L OFN 500 fg/L
Benzophenone DFTPP 100 pg/L Benzophenone DFTPP 100 pg/L Part No. 05970-60045
BFB BFB
GC and GC/MS
Description Evaluation Sample for GC/MS Systems Contains 6 vials: 4 vials (Sample A, 10 ng/L), 1 vial (Sample B, 100 pg/L), and 1 vial (Sample C, 100 ng/L) each of dodecane biphenyl, p-chlorodiphenyl, and methyl palmitate in isooctane, 1 mL ampoules GC/MS Tuning Standard contains: DFTPP, Benzidine, Pentachlorophenol, and p,p-DDT 1 mg/mL in methylene chloride 5989 Installation Sample Kit contains: HCB 50 pg/L, HCB 20 pg/mL, Benzophenone 100 pg/L, OFN 1 pg/L, mix of HCB, OFN and Benzophenone 5 ng/L Extended Mass Performance Sample, 19 g, fomblin oil PFTBA certified, 1 bottle, 10 g PFTBA Sample Kit, 0.5 mL PFDTD, 10 g Benzophenone, 100 pg/L, 5 ampoules Hexachlorobenzene, 10 pg/L, 4 ampoules 1 ng/L, 2 ampoules Hexachlorobenzene, 20 pg/L Methyl stearate (in methanol); 1 ng/L, 2 ea PFTBA not certified Octafluoronapthalene (OFN), 1 pg/L, 5 ampoules Octafluoronapthalene (OFN), 500 fg/L p-Bromofluorobenzene (BFB), 25 g/mL
8500-5995
8500-6406
8500-5500 8500-0656 05971-60571 8500-8130 8500-5440 8500-5808 8500-6405 05990-60075 8500-5441 8500-6572 8500-5851 www.agilent.com/chem
216
GC Columns The J&W Scientific Brand of Technically Advanced GC Columns

In 2000 Agilent Technologies merged with J&W Scientific. The worlds two largest column manufacturers combined to become one. HP- and DB- are both dependable GC column names that you have relied upon for over 25 years. Now you can find both in one box, under one brand nameJ&W Scientificfrom one company, Agilent Technologies. The box is new, but the quality production processes and your results will remain the same. Your J&W Scientific columns are backed by decades of chromatography experience and column manufacturing know-how. This enables us to provide superior-quality, dependable GC columns and unmatched service and support. The DB- and HP- capillary column product lines and the manufacturing operations have been consolidated in Folsom, California, USA. As always, every GC column meets the tighest quality control specifications in the industry. So if youre worried about the consistency of your next HP-5ms, DB-1, or any other column, rest assured that you will be getting the same GC columns youve been relying on for years. Our goal is to continually refine our materials and processes to provide the best columns in the world. And each one will continue to deliver the high quality and superior performance you have come to expect.
GC and GC/MS
With the Industrys Best Low-bleed Columns

Agilent invests heavily in R&D to develop superior-performing, low-bleed GC columns, gaining us respect industrywide as the low-bleed leader.
Wed rather be known for no bleed.

Which is precisely why were obsessed with reducing column bleed to the bare minimum and developing new stationary phase chemistries. Why all the concern about column bleed, you ask? Because column bleed is the degradation of the stationary-phase polymer that accelerates at a higher temperature, elevating the baseline as the temperature rises to the columns upper thermal limit. Ultimately, the signal-to-noise ratio dictates analytical sensitivities and detection limits. Decrease the noise and the sensitivity will increase, which is a good thing. Get rid of column bleed and you help eliminate the noise that can decrease MS spectral integrity, reduce instrument uptime, decrease column lifetime and lower reproducibility at trace levels.
217
GC Columns
Why low-bleed columns are a smart choice.
Standard 35%Phenyl
If you perform trace analyses where sensitivity and instrument performance are important, column bleed (or the lack thereof) should be important to you. Many GC detectors are sensitive to contamination from bleed. By using low-bleed columns, these detectors require less maintenance. Due to the unique relationship between ion storage capacity of the trap and sensitivity levels, ion trap MS users will especially benefit from low-bleed columns. J&W brands low-bleed GC columns also give you the advantages of superior inertness and increased upper temperature limits.
DB-35ms
10 12 14 16
Time (min)
What makes J&W Scientific low-bleed columns so special?

20 22
18
Primary Quant Ion is hidden
276
Standard 35% Phenyl
GC and GC/MS
In short, its J&Ws unique polymer chemistry and proprietary deactivation. Our MS columns utilize special surface deactivation and siloxane chemistries that enhance the chromatographic performance of siloxane polymers. To provide selectivities virtually identical to their conventional counterparts, we offer DB-5ms, DB-35ms, DB-17ms and DB-225ms. The HP-5ms uses the same stationary phase as the standard HP-5, providing low bleed without the use of arylene technology. DB-1ms and HP-1ms provides the same selectivity as the standard 100% dimethylpolysiloxane (DB-1 type) column. Plus, our unique DB-1ms polymer chemistry and proprietary deactivation techniques help us increase temperature limits (340/360), improving thermal stability and optimizing performance. Because the polysiloxane backbone of the stationary phase is optimized to yield the lowest-bleed column possible, DB-XLB doesnt have any conventional counterparts. You can gain sensitivity by replacing your general-purpose non-polar columns, such as 100%-dimethylpolysiloxane and 5%-diphenyl/95% dimethylpolysiloxane, with DB-XLB.
276
Primary Quant Ion is enhanced
With the Tightest QC Specs in the Business!

DB-35ms
One nanogram of benzo[g,h,i]perylene (m/z 276) at 340C on a standard 35%-phenyl column and on DB 35ms. The increased signal-to-noise ratio is apparent in the chromatograms (top), and the cleaner spectra (lower) will result in more confident peak identifications.
We make more capillary GC columns than anyone else in the world. And we still build them with our consistently rigid performance criteria, such as separation efficiency, retention characteristics, peak height ratios and bleed rates.
Better precision. For better results.

J&W Scientific columns adhere to very tight retention factor (k) specifications, helping ensure consistent retention from column to column. To assure separation reproducibility for the widest range of compounds, we measure at least three retention indices for each column. Narrow retention index ranges help ensure reproducible peak separations from column to column, while a wide range indicates a failure to precisely control stationary phase structure.
218
GC Columns
Higher performance. And nice and narrow peaks.
Theoretical plates per meter measures column efficiency (N). Columns with higher plate numbers generate the narrowest peaks, improving the resolution of closely eluting peaks. Paying close attention to the surface deactivation process and coating efficiency of the stationary phase is critical to obtain the highest number of theoretical plates.
Increased sensitivity. With very low bleed levels.

Bleed is the columns contribution to the background signal. The lowest-bleed columns provide the best analytical sensitivity, which is why we devote substantial research to reduce column bleed. We work hard to create the widest range of low-bleed stationary phases. Weve also improved our standard phase columns, resulting in even lower bleed levels.
Excellent peak shape.

Agilents stringent QC performance evaluation tests provide the utmost confidence in the qualitative and quantitative results, especially for difficult to chromatograph compounds. We measure peak height ratios for acidic and basic compounds to ensure the highest column performance for the widest range and types of compounds. We also carefully monitor peak symmetry and tailing for a broad range of chemically active compounds.
GC and GC/MS
What Do Our Tighter Performance Criteria Do For You?

Feature Narrow Retention Index and Retention Factor Window Increased Plates per Meter Lowest Bleed Advantage Highest level of column-to-column reproducibility Minimal method adjustment when changing columns Highest level of resolving power Improved sample throughput Increased analytical sensitivity for all detectors Fast baseline stabilization Faster column exchange Excellent thermal stability Benefit Confidence in analytical results Improved sample throughput, reduced downtime Accurate quantification Potentially shorter run times Improved detection limits Reliable compound identification Minimized conditioning time Increased sample throughput Increased column lifetime, reduced downtime Increased sample throughput
Specify J&W chemistry for intra-company methodologies Confidence in method transfer and intra-company results
219
GC Columns
Choosing a Column
Choosing a Capillary GC Column

Selecting the best capillary column for an analysis can be an uncertain and sometimes difficult task. The first step should always be to refer to what has already been done. Our chemists have put together a variety of resources to help you find the right column for your analysis. Take a look at:
Chromatograms
Our chemists continue to evaluate and develop methods for a multitude of applications. Weve put together some of the more common chromatograms with column recommendations and method parameters for your reference in this catalog (pages 347441). If you dont find what youre looking for, take a look on our website for a more extensive chromatogram library and a compound search function (go to www.agilent.com/chem, then click on Library).
Method Guides
Weve evaluated the most common Environmental/EPA methods, ASTM methods, and general compound analyses and have put together simple guides which specify the best column recommendation for these methods. These are available for your reference on pages 315323.
Retention Data Lists
GC and GC/MS
Take a look at our solvent and pesticide retention data lists (pages 341347). Weve analyzed hundreds of compounds on several different phases to help you determine which column will be the best choice for your list of compounds.
Reference Section
If you are still unable to find an existing method for your application, take a look at our reference section on pages 324340. While there are no foolproof techniques, shortcuts, tricks or secrets to column selection, there are some guidelines. Our reference guide gives you helpful hints for choosing a stationary phase, picking the right column dimensions, developing temperature programs and determining the right inlet and detector for the application.
Contact Us
Even though weve developed many reference tools to help you find the right column, we are always ready and happy to give the best column recommendation and method development advice possible. Our Technical Support Chemists have over a 100 years of combined experience running samples and developing methods. We are the gas chromatography column experts and we are at your disposal! Send in questions on the Technical Support question form on our website www.agilent.com/chem or contact your local Agilent office.
220
GC Columns
Columns for GC/MS
Columns for GC/MS

There is a rapidly increasing population of benchtop GC/MS instruments in analytical laboratories that analyze a broadening range of trace level, higher temperature samples. These samples require increasingly inert, lower bleed, higher temperature columns. In response to this growing need, Agilent Technologies deliberately designed several ms columns to chromatograph a broader range of low level samples and generating lower bleed even at higher temperatures. What makes a J&W low bleed column from Agilent unique? Unique polymer chemistry and proprietary surface deactivation, both of which have contributed to columns that adhere to the tightest quality control specifications in the industry for bleed, inertness, selectivity and efficiency. J&W ms columns utilize special surface deactivation and siloxane chemistries which enhance the chromatographic performance of siloxane polymers.
DB-1ms
100% Dimethylpolysiloxane, identical selectivity to DB-1 Non-polar Very low bleed characteristics, ideal for GC/MS Improved acid performance compared to standard 100% Dimethylpolysiloxane columns Improved signal-to-noise ratio for better sensitivity and mass spectral integrity 340/360C upper temperature limit Excellent general purpose column Bonded and cross-linked Solvent rinsable Test mix available. See pages 9697 for test mix information. Similar Phases: HP-1ms, Rtx-1ms, CP-Sil 5 CB Low Bleed/MS, MDN-1, AT-1
CH O Si CH
3 3
100%
GC and GC/MS
Structure of Dimethylpolysiloxane
DB-1ms Chromatograms
Drug Screen page 416
DB-1ms
ID (mm) Length (m) Film (m) Temp Limits (C) Part No.
0.10 0.10 0.10 0.10 0.20 0.20
10 10 20 20 12 25
0.10 0.40 0.10 0.40 0.33 0.33
-60 to 340/360 -60 to 340/360 -60 to 340/360 -60 to 340/360 -60 to 340/360 -60 to 340/360
127-0112 127-0113 127-0122 127-0123 128-0112 128-0122
221
GC Columns
Columns for GC/MS
DB-1ms (continued)
0.25 0.25 0.25 0.25 0.32 0.32 0.32 0.32
15 30 30 60 15 30 30 60
0.25 0.10 0.25 0.25 0.25 0.10 0.25 0.25
-60 to 340/360 -60 to 340/360 -60 to 340/360 -60 to 340/360 -60 to 340/360 -60 to 340/360 -60 to 340/360 -60 to 340/360
122-0112 122-0131 122-0132 122-0162 123-0112 123-0131 123-0132 123-0162
HP-1ms
CH O Si CH
3 3
100%
GC and GC/MS
100% Dimethylpolysiloxane Identical selectivity to HP-1 Non-polar Low bleed characteristics Excellent general purpose column Improved signal-to-noise ratio for better sensitivity and mass spectral integrity Bonded and cross-linked Solvent rinsable Similar Phases: HP-1ms, Rtx-1ms, CP-Sil 5 CB Low Bleed/MS, MDN-1, AT-1
HP-1ms
0.20 0.25 0.25 0.25 0.25 0.25 0.25 0.32 0.32 0.32 0.32 0.32 0.32
25 15 30 30 30 30 60 15 25 30 30 30 60
0.33 0.25 0.10 0.25 0.50 1.00 0.25 0.25 0.52 0.10 0.25 1.00 0.25
-60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350
19091S-602 19091S-931 19091S-833 19091S-933 19091S-633 19091S-733 19091S-936 19091S-911 19091S-612 19091S-813 19091S-913 19091S-713 19091S-916
222
GC Columns
Columns for GC/MS
DB-5ms
Phenyl Arylene polymer virtually equivalent to a (5%-Phenyl)-methylpolysiloxane Non-polar Very low bleed characteristics, ideal for GC/MS Excellent inertness for active compounds Improved signal-to-noise ratio for better sensitivity and mass spectral integrity Bonded and cross-linked Solvent rinsable Ion trap testing and certification available Exact replacement of HP-5TA Close equivalent to USP Phase G27 Test mix available. See pages 9697 for test mix information. Similar Phases: Rtx-5ms, PTE-5, CP-Sil 8 CB Low Bleed/MS, BPX-5, AT-5ms
CH O Si CH
3 3
CH Si CH
CH O Si CH
3 m
Structure of poly(dimethylsiloxy)poly(1,4-bis(dimethylsiloxy) phenylene)siloxane
50 ppb Formaldehyde page 380 Amines and Nitriles page 397 Diesel Fuel page 348 EPA Air Analysis Compendium page 379 Method TO-15 (1 ppV Standard) EPA Method 525.2 page 367 EPA Method 551.1 page 377 EPA Method 8061 page 368 (Phthalate Esters) Narcotics page 420 Organochlorine Pesticides page 350 (EPA Method 8081A) Organophosphorous Pesticides page 356 (EPA Method 8141 A) Phenols pages 369, 412 Polyethyleneamines page 396 Substituted Anilines page 411 Sulfur in Air page 380
GC and GC/MS
DB-5ms
0.18 0.18 0.20 0.20 0.20 0.25 0.25 0.25 0.25 0.25 0.25
20 40 12 25 50 15 15 15 15 25 25
0.18 0.18 0.33 0.33 0.33 0.10 0.25 0.50 1.00 0.25 0.40
-60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350
121-5522 121-5542 128-5512 128-5522 128-5552 122-5511 122-5512 122-5516 122-5513 122-5522 122-552A
223
GC Columns
Columns for GC/MS
DB-5ms (continued)
0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32
30 30 30 30 50 60 60 60 15 15 15 25 30 30 30 30 50 50 60 60 60 60 15 15 15 30 30 30
0.10 0.25 0.50 1.00 0.25 0.10 0.25 1.00 0.10 0.25 1.00 0.52 0.10 0.25 0.50 1.00 0.25 1.00 0.10 0.25 0.50 1.00 0.50 1.00 1.50 0.50 1.00 1.50
-60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 300/320 -60 to 300/320 -60 to 300/320 -60 to 300/320 -60 to 300/320 -60 to 300/320
122-5531 122-5532 122-5536 122-5533 122-5552 122-5561 122-5562 122-5563 123-5511 123-5512 123-5513 123-5526 123-5531 123-5532 123-5536 123-5533 123-5552 123-5553 123-5561 123-5562 123-5566 123-5563 125-5517 125-551J 125-5512 125-5537 125-553J 125-5532
GC and GC/MS
0.32 0.32 0.32 0.32 0.32 0.32 0.53 0.53 0.53 0.53 0.53 0.53
DB-5ms.ITD
0.25 30 0.25 -60 to 325/350 122-4132
224
GC Columns
Columns for GC/MS
HP-5ms
(5%-Phenyl)-methylpolysiloxane Identical selectivity to HP-5 Non-polar Very low bleed characteristics, ideal for GC/MS Excellent inertness for active compounds including acidic and basic compounds Improved signal-to-noise ratio for better sensitivity and mass spectral integrity Bonded and cross-linked Solvent rinsable Equivalent to USP Phase G27 Similar Phases: Rtx-5MS, Rtx-5 Amine, DB-5ms, PTE-5, CP-Sil 8CB Low Bleed/MS, BPX-5
CH 3 O Si CH 3
95%
C6H5 O Si C6 H5
5%
Structure of Diphenyldimethylpolysiloxane
HP-5ms Chromatograms
Chlorinated Pesticides: EPA page 352 Method 508 Nitrogen/Phosphorus Containing page 356 Pesticides: EPA Method 507 Organohalide Pesticides in page 352 Water, EPA Method 505 Phenols on HP-5MS page 412 USEPA Method 8270 page 366 Semivolatile Compounds
GC and GC/MS
HP-5ms
0.20 0.20 0.20 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.25
12 25 50 15 15 15 30 30 30 30 60 60
0.33 0.33 0.33 0.10 0.25 1.00 0.10 0.25 0.50 1.00 0.10 0.25
-60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350
19091S-101 19091S-102 19091S-105 19091S-331 19091S-431 19091S-231 19091S-333 19091S-433 19091S-133 19091S-233 19091S-336 19091S-436
225
GC Columns
Columns for GC/MS
HP-5ms (continued)
0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32
15 15 15 25 30 30 30 30 60 60
0.10 0.25 1.00 0.52 0.10 0.25 0.50 1.00 0.10 0.25
-60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350
19091S-311 19091S-411 19091S-211 19091S-112 19091S-313 19091S-413 19091S-113 19091S-213 19091S-316 19091S-416
DB-XLB
EXceptionally Low Bleed Low polarity Extended temperature limit of 340/360C Unique selectivity Excellent inertness for active compounds Ideal for confirmational analyses Excellent for pesticides, herbicides, PCBs and PAHs Ideal for GC/MS Ion trap testing and certification available Bonded and cross-linked Solvent rinsable Test mix available. See pages 9697 for test mix information. Similar Phase: MDN-12 Note: DB-XLB is designed for inhibiting column bleed at high temperatures. It also appears to have inadvertently inherited an exceptional ability for separating many PCB congeners when used with MS detection. This stellar performance was maximized after careful optimization of the column dimensions, temperature programs, and carrier gas flow conditions... (Frame, G. Analytical Chemistry News & Features, Aug. 1, 1997, 468A-475A)
GC and GC/MS
226
GC Columns
Columns for GC/MS
DB-XLB Chromatograms
Aroclors 1016-1268 (without 1221) CLP Pesticides PCB Congeners in DIN Method EPA 508.1 Pesticides EPA 8151A Phenoxy Acid Herbicides - Methyl Derivatives page 361 page 349 page 362 page 351 page 357 EPA 552.2 Garlic Oil Herbicides PCBs by EPA Method 8082 Phenols Ylang Ylang Oil page 370 page 384 page 358 page 363 page 369 page 386
DB-XLB
0.18 0.18 0.18 0.20 0.20 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.45 0.53 0.53
20 30 60 12 25 15 15 15 30 30 30 30 60 15 15 30 30 30 30 60 30 15 30
0.18 0.18 0.18 0.33 0.33 0.10 0.25 1.00 0.10 0.25 0.50 1.00 0.25 0.25 1.00 0.10 0.25 0.50 1.00 0.25 1.27 1.50 1.50
30 to 340/360 30 to 340/360 30 to 340/360 30 to 340/360 30 to 340/360 30 to 340/360 30 to 340/360 30 to 340/360 30 to 340/360 30 to 340/360 30 to 340/360 30 to 340/360 30 to 340/360 30 to 340/360 30 to 340/360 30 to 340/360 30 to 340/360 30 to 340/360 30 to 340/360 30 to 340/360 30 to 320/340 30 to 320/340 30 to 320/340
121-1222 121-1232 121-1262 128-1212 128-1222 122-1211 122-1212 122-1213 122-1231
GC and GC/MS
122-1232 122-1236 122-1233 122-1262 123-1212 123-1213 123-1231 123-1232 123-1236 123-1233 123-1262 124-1232* 125-1212 125-1232
*No special ferrules required for high speed 0.45 mm id columns
DB-XLB.ITD
.25 30 .25 30 to 340/360 122-4232
227
GC Columns
Columns for GC/MS
DB-35ms
Virtually equivalent to a (35%-Phenyl)-methylpolysiloxane Midpolarity Very low bleed characteristics, ideal for GC/MS Extended temperature limit of 340/360C Excellent inertness for active compounds Ideal for confirmational analyses Bonded and cross-linked Solvent rinsable Replaces HP-35ms Close equivalent to USP Phase G42 Test mix available. See pages 96-97 for test mix information. Similar Phases: Rtx-35, SPB-35, AT-35, Sup-Herb, MDN-35, BPX-35
Anilines Barbiturates Benzodiazepines Chlordane CLP Pesticides EPA 508.1 Pesticides EPA 552.2 EPA 8151A Phenoxy Acid Herbicides - Methyl Derivatives page 411 page 418 page 419 page 364 page 349 page 351 page 370 page 357 Organochlorine Pesticides (EPA Method 8081A) Organophosphorous Pesticides (EPA Method 8141 A) PCBs by EPA Method 8082 Toxaphene page 350 page 357 page 363 page 364
GC and GC/MS
DB-35ms
0.20 0.20 0.25 0.25 0.25 0.25 0.32 0.32 0.32 0.53 0.53 0.53
15 25 15 30 30 60 15 30 60 15 30 30
0.33 0.33 0.25 0.15 0.25 0.25 0.25 0.25 0.25 1.00 0.50 1.00
50 to 340/360 50 to 340/360 50 to 340/360 50 to 340/360 50 to 340/360 50 to 340/360 50 to 340/360 50 to 340/360 50 to 340/360 50 to 320/340 50 to 320/340 50 to 320/340
128-3812 128-3822 122-3812 122-3831 122-3832 122-3862 123-3812 123-3832 123-3862 125-3812 125-3837 125-3832
228
GC Columns
Columns for GC/MS
DB-17ms
Virtually equivalent to (50%-Phenyl)-methylpolysiloxane 320/340C upper temperature limit Very low bleed midpolarity column, ideal for GC/MS Excellent inertness for active compounds Enhanced mass spectral integrity Bonded and cross-linked Solvent rinsable Best column for CLP pesticides Test mix available. See pages 9697 for test mix information. Similar Phases: HP-50+, Rtx-50, 007-17, SP-2250, SPB-50, BPX-50, SPB-17, AT-50
Hallucinogens PAHs Tocopherols page 420 page 368 page 419
DB-17ms
0.18 0.25 0.25 0.25 0.25 0.25 0.32 0.32 0.32 0.32
20 15 15 30 30 60 15 15 30 30
0.18 0.15 0.25 0.15 0.25 0.25 0.15 0.25 0.15 0.25
40 to 320/340 40 to 320/340 40 to 320/340 40 to 320/340 40 to 320/340 40 to 320/340 40 to 320/340 40 to 320/340 40 to 320/340 40 to 320/340
121-4722
GC and GC/MS
122-4711 122-4712 122-4731 122-4732 122-4762 123-4711 123-4712 123-4731 123-4732
229
GC Columns
Columns for GC/MS
DB-225ms
Virtually equivalent to (50%-Cyanopropylphenyl)-methylpolysiloxane Mid/high polarity Excellent for separations of cis- and trans-fatty acid methyl esters (FAMEs) Low bleed Bonded and cross-linked Solvent rinsable Close equivalent to USP Phase G7 Similar Phases: HP-225, SP-2330, CP-Sil 43 CB, Rtx-225, BP-225, OV-225, 007-225, AT-225
Tetrachlorodibenzo-p-furans page 359
DB-225ms
0.25 0.25 0.25 0.32 0.32
15 30 60 15 30
0.25 0.25 0.25 0.25 0.25
40 to 240 40 to 240 40 to 240 40 to 240 40 to 240
122-2912 122-2932 122-2962 123-2912 123-2932
GC and GC/MS
230
GC Columns
Polysiloxane Polymers
General Purpose Stationary Phases

Agilent makes more capillary columns than anyone else in the world and our commitment to product reliability and repeatability has raised the level of quality that chromatographers demand. We still continue to tighten our performance criteria such as separation efficiency, retention characteristics, peak height ratios and bleed rates. Our vast selection of high quality products is sure to meet the chromatographic needs for virtually any application.
Polysiloxanes are the most common stationary phases. They are available in the greatest variety and are stable, robust and versatile. Standard polysiloxanes are characterized by the repeating siloxane backbone. Each silicon atom contains two functional groups. The type and amount of the groups distinguish each stationary phase and its properties. Youll also find our GC/MS phases listed in this section. While some of the GC/MS phases utilize different polymer chemistries, their selectivities mimic the standard polysiloxane phases and offer the advantage of low bleed and in some cases extended temperature ranges. With the merger of Agilent and J&W Scientific there were many similar columns with the same type of polymer. In some cases the manufacturing and QC processes were exactly the same. In these cases the DB version was kept. In the cases where the HP and the DB columns had any manufacturing or QC differences, we opted to keep both phases available as in the case of DB-1 and HP-1. Each of these columns is a high quality product made to meet exacting quality control testing. However there may be some subtle performance differences. For example the DB-35 and the HP-35 have slightly different selectivities. Therefore, we are still offering both the DB and the HP version for our customers who have methods already developed on these columns.
R O Si R
n
R=CH3 CH2CH2CH2CN CH2CH2CF3
methyl cyanopropyl trifluoropropyl phenyl

Polysiloxanes
GC and GC/MS
DB-1
100% Dimethylpolysiloxane Non-polar Excellent general purpose column Wide range of applications Low bleed High temperature limit Bonded and cross-linked Solvent rinsable Wide range of column dimensions available Equivalent to USP Phase G2 Test mix available. See pages 9697 for test mix information. Similar Phases: HP-1, Ultra-1, SPB-1, CP-Sil 5 CB Low Bleed/MS, Rtx-1, BP-1, OV-1, OV-101, 007-1(MS), SP-2100, SE-30, CP-Sil 5 CB MS, ZB-1, AT-1, MDN-1
O
CH Si CH
100%
231
GC Columns
DB-1 Chromatograms
Aldehydes and Ketones Anabolic Steroids Anticonvulsants Aromatics Aromatics in Finished Gasoline ASTM Method 5769 EPA Air Analysis Compendium Method TO-14 Standard EPA Method 551 Esters Formaldehyde - DNPH Derivative Formaldehyde - PFBHA Derivative page 398 page 422 page 417 page 399 page 439 page 378 page 376 page 402 page 399 page 399 Fragrance Reference Standard page 381 Glycols page 404 Halogenated Hydrocarbons page 405 Nitrogen Based Solvents page 409 Polyethylene page 441 Pyrethrins page 364 Regular Unleaded Gasoline page 440 (California Phase 1) "Fast" GC Run Triethylene Glycol and Impurities page 404 Volatile Amines page 396 Volatile Sulfur Compounds page 432
DB-1
0.05 0.05 0.10 0.10 0.10 0.10 0.10 0.10 0.10 0.10 0.15 0.15 0.18 0.18 0.18 0.18 0.18 0.18 0.18 0.20 0.20 0.20 0.20 0.25
10 10 5 10 10 10 20 20 40 40 10 25 10 10 10 20 20 20 40 12 15 25 50 10
0.05 0.20 0.12 0.10 0.12 0.40 0.10 0.40 0.20 0.40 1.20 1.20 0.18 0.20 0.40 0.18 0.20 0.40 0.40 0.33 0.20 0.33 0.33 0.12
-60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 280/300 -60 to 280/300 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350
126-1012 126-1013 127-100A 127-1012 127-101A 127-1013 127-1022 127-1023 127-1046 127-1043 12A-1015 12A-1025 121-1012 121-101A 121-1013 121-1022 121-102A 121-1023 121-1043 128-1012 128-10H7 128-1022 128-1052 122-10H7
GC and GC/MS
232
GC Columns
DB-1 (continued)
0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32
15 15 15 15 25 30 30 30 30 50 60 60 60 60 100 105 150 15 15 15 15 15 15 25 25 25 25 30 30 30 30 30 30 30 30 50 50
0.10 0.25 0.50 1.00 0.25 0.10 0.25 0.50 1.00 0.25 0.10 0.25 0.50 1.00 0.50 0.50 1.00 0.10 0.25 0.50 1.00 3.00 5.00 0.12 0.25 0.52 1.05 0.10 0.25 0.50 1.00 1.50 2.00 3.00 5.00 0.25 0.52
-60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 280/300 -60 to 280/300 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 300/320 -60 to 280/300 -60 to 280/300 -60 to 280/300 -60 to 325/350 -60 to 325/350
122-1011 122-1012 122-101E 122-1013 122-1022 122-1031 122-1032 122-103E 122-1033 122-1052 122-1061 122-1062 122-106E 122-1063 122-10AE 122-10BE
GC and GC/MS
122-10G3 123-1011 123-1012 123-101E 123-1013 123-1014 123-1015 123-1027 123-1022 123-1026 123-102F 123-1031 123-1032 123-1033E 123-1033 123-103B 123-103G 123-1034 123-1035 123-1052 123-1056
233
GC Columns
DB-1 (continued)
0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.45 0.45 0.45 0.45 0.45
50 50 50 60 60 60 60 60 60 60 60 15 15 30 30 30 30 60 5 5 10 10 15 15 15 15 15 15 15 25 25 30 30 30
1.05 1.20 5.00 0.10 0.25 0.50 1.00 1.50 2.00 3.00 5.00 1.27 2.55 0.42 1.27 2.55 4.25 1.27 2.65 5.00 2.65 5.00 0.15 0.25 0.50 1.00 1.50 3.00 5.00 1.00 5.00 0.10 0.25 0.50
-60 to 325/350 -60 to 325/350 -60 to 280/300 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 300/320 -60 to 280/300 -60 to 280/300 -60 to 280/300 -60 to 300/320 -60 to 260/280 -60 to 300/320 -60 to 300/320 -60 to 260/280 -60 to 260/280 -60 to 300/320 -60 to 260/280 -60 to 260/280 -60 to 260/280 -60 to 260/280 -60 to 340/360 -60 to 320/340 -60 to 300/320 -60 to 300/320 -60 to 300/320 -60 to 260/280 -60 to 260/280 -60 to 300/320 -60 to 260/280 -60 to 340/360 -60 to 320/340 -60 to 300/320
123-105F 123-105C 123-1055 123-1061 123-1062 123-106E 123-1063 123-106B 123-106G 123-1064 123-1065 124-1012* 124-1014* 124-1037* 124-1032* 124-1034* 124-1005* 124-1062* 125-100B 125-1005 125-10HB 125-10H5 125-1011 125-101K 125-1017 125-101J 125-1012 125-1014 125-1015 125-102J 125-1025 125-1039 125-103K 125-1037
GC and GC/MS
0.45 0.45 0.53 0.53 0.53 0.53 0.53 0.53 0.53 0.53 0.53 0.53 0.53 0.53 0.53 0.53 0.53 0.53
*No special ferrules required for high speed megabore 0.45 mm id columns
234
GC Columns
DB-1 Order Guide (continued)
0.53 0.53 0.53 0.53 0.53 0.53 0.53 0.53 0.53 0.53 0.53 0.53
30 30 30 30 30 30 50 60 60 60 60 105
0.88 1.00 1.50 2.65 3.00 5.00 5.00 1.00 1.50 3.00 5.00 5.00
125-103D 125-103J 125-1032 125-103B 125-1034 125-1035 125-1055 125-106J 125-1062 125-1064 125-1065 125-10B5
HP-1
100% Dimethylpolysiloxane Non-polar Excellent general purpose columnIndustry Standard Wide range of applications Superior performance for low molecular weight alcohols (<C5) High temperature limit Bonded and cross-linked Solvent rinsable Wide range of column dimensions available Equivalent to USP Phase G2 Similar Phases: DB-1, Ultra-1, SPB-1, CP-Sil 5 CB, Rtx-1, BP-1, OV-1, OV-101, 007-1(MS), SP-2100, SE-30, CP-Sil 5 CB MS, ZB-1, AT-1, MDN-1
CH O Si CH
3 3
GC and GC/MS
100%
HP-1 Chromatograms
Common Industrial Solvents Denatured Fuel Ethanol-ASTM D5501 Glycols/Diols Inorganic Hydride Gases Organotin Compounds page 408 page 437 page 404 page 414 page 365 Oxygenates in Gasoline ASTM D5599 (GC-FID) Solvents Sulfur Compounds in Natural Gas Synthetic Mixture page 436 page 408 page 434
HP-1
0.20 0.20 0.20 0.20 0.20
12 25 25 25 50
0.33 0.11 0.33 0.50 0.11
-60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350
19091-60312 19091Z-002 19091Z-102 19091Z-202 19091Z-005
235
GC Columns
HP-1 (continued)
0.20 0.20 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.32 0.32 0.32 0.32 0.32
50 50 15 15 15 30 30 30 60 60 100 15 15 25 25 25 30 30 30 30 30 30 50 50 50 60 60 60 5 5 5 7.5 10 10 15 15 15 15
0.33 0.50 0.10 0.25 1.00 0.10 0.25 1.00 0.25 1.00 0.50 0.25 1.00 0.17 0.52 1.05 0.10 0.25 1.00 3.00 4.00 5.00 0.17 0.52 1.05 0.25 1.00 5.00 0.15 0.88 2.65 5.00 0.88 2.65 0.15 1.50 3.00 5.00
-60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 260/280 -60 to 260/280 -60 to 260/280 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 260/280 -60 to 320/400 -60 to 320/400 -60 to 260/280 -60 to 260/280 -60 to 300/320 -60 to 260/280 -60 to 320/400 -60 to 300/320 -60 to 260/280 -60 to 260/280
19091Z-105 19091Z-205 19091Z-331 19091Z-431 19091Z-231 19091Z-333 19091Z-433 19091Z-233 19091Z-436 19091Z-236 19091Z-530 19091Z-411 19091Z-211 19091Z-012 19091Z-112 19091Z-212 19091Z-313 19091Z-413 19091Z-213 19091Z-513 19091Z-613 19091Z-713 19091Z-015 19091Z-115 19091Z-215 19091Z-416 19091Z-216 19091Z-716 19095Z-220 19095Z-020 19095S-100 19095Z-627 19095Z-021 19095Z-121 19095Z-221 19095Z-321 19095Z-421 19095Z-621
GC and GC/MS
0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.53 0.53 0.53 0.53 0.53 0.53 0.53 0.53 0.53 0.53
236
GC Columns
HP-1 (continued)
0.53 0.53 0.53 0.53 0.53 0.53
30 30 30 30 30 60
0.88 1.50 2.65 3.00 5.00 5.00
-60 to 300/320 -60 to 300/320 -60 to 260/280 -60 to 260/280 -60 to 260/280 -60 to 260/280
19095Z-023 19095Z-323 19095Z-123 19095Z-423 19095Z-623 19095Z-626
DB-1ms
100% Dimethylpolysiloxane Identical selectivity to DB-1 Non-polar Very low bleed characteristics, ideal for GC/MS Improved acid performance compared to standard 100% Dimethylpolysiloxane columns Improved signal-to-noise ratio for better sensitivity and mass spectral integrity 340/360C upper temperature limit Excellent general purpose column Bonded and cross-linked Solvent rinsable Test mix available. See pages 9697 for test mix information. Similar Phases: HP-1ms, Rtx-1ms, CP-Sil 5 CB Low Bleed/MS, MDN-1, AT-1
CH Si CH
100%
GC and GC/MS
Drug Screen
page 416
DB-1ms
0.10 0.10 0.10 0.10 0.20 0.20 0.25 0.25 0.25 0.25 0.32 0.32 0.32 0.32
10 10 20 20 12 25 15 30 30 60 15 30 30 60
0.10 0.40 0.10 0.40 0.33 0.33 0.25 0.10 0.25 0.25 0.25 0.10 0.25 0.25
-60 to 340/360 -60 to 340/360 -60 to 340/360 -60 to 340/360 -60 to 340/360 -60 to 340/360 -60 to 340/360 -60 to 340/360 -60 to 340/360 -60 to 340/360 -60 to 340/360 -60 to 340/360 -60 to 340/360 -60 to 340/360
127-0112 127-0113 127-0122 127-0123 128-0112 128-0122 122-0112 122-0131 122-0132 122-0162 123-0112 123-0131 123-0132 123-0162
237
GC Columns
CH Si CH
HP-1ms
100%
100% Dimethylpolysiloxane Identical selectivity to HP-1 Non-polar Low bleed characteristics Excellent general purpose column Improved signal-to-noise ratio for better sensitivity and mass spectral integrity Bonded and cross-linked Solvent rinsable Similar Phases: HP-1ms, Rtx-1ms, CP-Sil 5 CB Low Bleed/MS, MDN-1, AT-1
HP-1ms
0.20 0.25 0.25 0.25 0.25 0.25 0.25 0.32 0.32 0.32 0.32 0.32 0.32
25 15 30 30 30 30 60 15 25 30 30 30 60
0.33 0.25 0.10 0.25 0.50 1.00 0.25 0.25 0.52 0.10 0.25 1.00 0.25
-60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350
19091S-602 19091S-931 19091S-833 19091S-933 19091S-633 19091S-733 19091S-936 19091S-911 19091S-612 19091S-813 19091S-913 19091S-713 19091S-916
GC and GC/MS
DB-5
CH 3 O Si CH 3
95%
C6H5 O Si C6 H5
5%
(5%-Phenyl)-methylpolysiloxane Non-polar Excellent general purpose column Wide range of applications Low bleed High temperature limit Bonded and cross-linked Solvent rinsable Wide range of column dimensions available Equivalent to USP Phase G27 Test mix available. See pages 9697 for test mix information. Similar Phases: HP-5, Ultra-2, SPB-5, CP-Sil 8CB, Rtx-5, BP-5, OV-5, 007-2(MPS-5), SE-52, SE-54, XTI-5, PTE-5, HP-5MS, ZB-5, AT-5, MDN-5
238
GC Columns
DB-5 Chromatograms
Amphetamines and PrecursorsTMS derivatives Antihistamines Bacterial Fatty Acid Methyl Esters Cold-Pressed Orange Oil Common Drug Screen page 416 page 417 page 390 page 385 page 415 9-THC (Marijuana) and Major Metabolites-TMS Derivatives Lemon Oil Narcotics and Adulterants Over-the-Counter Pain KillersTMS Derivatives Organochlorine Pesticides page 422 page 384 page 421 page 421 page 353
DB-5
0.05 0.05 0.10 0.10 0.10 0.10 0.10 0.10 0.10 0.10 0.15 0.18 0.18 0.18 0.18 0.18 0.18 0.20 0.20 0.20 0.20 0.20 0.20 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.25
10 10 5 10 10 10 10 20 20 40 10 10 10 20 20 40 40 12 15 25 25 50 50 15 15 15 15 25 25 30 30
0.05 0.20 0.12 0.10 0.17 0.33 0.40 0.10 0.40 0.20 1.20 0.18 0.40 0.18 0.40 0.18 0.40 0.33 0.20 0.11 0.33 0.11 0.33 0.10 0.25 0.50 1.00 0.25 1.20 0.10 0.25
-60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 300/320 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 300/320 -60 to 325/350 -60 to 325/350
126-5012 126-5013 127-500A 127-5012 127-501E 127-501N 127-5013 127-5022 127-5023
GC and GC/MS
127-5046 12A-5015 121-5012 121-5013 121-5022 121-5023 121-5042 121-5043 128-5012 128-50H7 128-5021 128-5022 128-5051 128-5052 122-5011 122-5012 122-501E 122-5013 122-5022 122-502C 122-5031 122-5032
239
GC Columns
DB-5 (continued)
0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32
30 30 50 60 60 60 60 10 15 15 15 15 25 25 25 25 30 30 30 30 30 50 50 50 50 50 60 60 60 60 15 30 30 30 10 10 10
0.50 1.00 0.25 0.10 0.25 0.50 1.00 0.25 0.10 0.25 0.50 1.00 0.17 0.25 0.52 1.05 0.10 0.25 0.50 1.00 1.50 0.17 0.25 0.52 1.00 1.05 0.10 0.25 0.50 1.00 1.27 0.42 1.27 4.25 2.00 2.65 5.00
-60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 300/320 -60 to 300/320 -60 to 300/320 -60 to 260/280 -60 to 300/320 -60 to 260/280 -60 to 260/280
122-503E 122-5033 122-5052 122-5061 122-5062 122-506E 122-5063 123-5002 123-5011 123-5012 123-501E 123-5013 123-502D 123-5022 123-5026 123-502F 123-5031 123-5032 123-503E 123-5033 123-503B 123-505D 123-5052 123-5056 123-5053 123-505f 123-5061 123-5062 123-506E 123-5063 124-5012* 124-5037* 124-5032* 124-5035* 125-50H3 125-50HB 125-50H5
GC and GC/MS
0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.45 0.45 0.45 0.45 0.53 0.53 0.53
240
GC Columns
DB-5 (continued)
0.53 0.53 0.53 0.53 0.53 0.53 0.53 0.53 0.53 0.53 0.53 0.53 0.53 0.53 0.53 0.53 0.53 0.53 0.53 0.53
15 15 15 15 15 15 25 25 30 30 30 30 30 30 30 30 50 60 60 60
0.25 0.50 1.00 1.50 3.00 5.00 2.00 5.00 0.25 0.50 0.88 1.00 1.50 2.65 3.00 5.00 1.00 1.50 3.00 5.00
-60 to 300/320 -60 to 300/320 -60 to 300/320 -60 to 300/320 -60 to 260/280 -60 to 260/280 -60 to 300/320 -60 to 260/280 -60 to 300/320 -60 to 300/320 -60 to 300/320 -60 to 300/320 -60 to 300/320 -60 to 260/280 -60 to 260/280 -60 to 260/280 -60 to 300/320 -60 to 300/320 -60 to 260/280 -60 to 260/280
125-501K 125-5017 125-501J 125-5012 125-5014 125-5015 125-5023 125-5025 125-503K 125-5037 125-503D 125-503J 125-5032 125-503B 125-5034 125-5035
GC and GC/MS
125-505J 125-5062 125-5064 125-5065
HP-5
(5%-Phenyl)-methylpolysiloxane Non-polar Excellent general purpose column Wide range of applications High temperature limit Bonded and cross-linked Solvent rinsable Wide range of column dimensions available Equivalent to USP Phase G27 Similar Phases: DB-5, Ultra-2, SPB-5, CP-Sil 8 CB, Rtx-5, BP-5, OV-5, 007-2(MPS-5), SE-52, SE-54, XTI-5, PTE-5, HP-5MS, ZB-5, AT-5, MDN-5
CH 3 O Si CH 3
95%
C6H5 O Si C6 H5
5%
HP-5 Chromatograms
Organomercury Compounds Organotin Compounds
page 365 page 365
241
GC Columns
HP-5
0.20 0.20 0.20 0.20 0.20 0.20 0.20 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.32 0.32
12 25 25 25 50 50 50 15 15 30 30 30 60 60 15 15 25 25 25 30 30 30 30 30 50 50 50 60 60 10 15 15 30 30 30 30
0.33 0.11 0.33 0.50 0.11 0.33 0.50 0.25 1.00 0.10 0.25 1.00 0.25 1.00 0.25 1.00 0.17 0.52 1.05 0.10 0.25 0.50 1.00 1.00 0.17 0.52 1.05 0.25 1.00 2.65 1.50 5.00 0.88 1.50 2.65 5.00
-60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 260/280 60 to 300/320 -60 to 260/280 60 to 300/320 60 to 300/320 -60 to 260/280 -60 to 260/280
19091J-101 19091J-002 19091J-102 19091J-202 19091J-005 19091J-105 19091J-205 19091J-431 19091J-231 19091J-333 19091J-433 19091J-233 19091J-436 19091J-236 19091J-411 19091J-211 19091J-012 19091J-112 19091J-212 19091J-313 19091J-413 19091J-113 19091J-213 19091J-213E 19091J-015 19091J-115E 19091J-215 19091J-416 19091J-216 19095J-121 19095J-321 19095J-621 19095J-023 19095J-323 19095J-123 19095J-623
GC and GC/MS
0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.53 0.53 0.53 0.53 0.53 0.53 0.53
242
GC Columns
DB-5ms
Phenyl Arylene polymer virtually equivalent to a (5%-Phenyl)-methylpolysiloxane Non-polar Very low bleed characteristics, ideal for GC/MS Excellent inertness for active compounds Improved signal-to-noise ratio for better sensitivity and mass spectral integrity Bonded and cross-linked Solvent rinsable Ion trap testing and certification available Exact replacement of HP-5TA Close equivalent to USP Phase G27 Test mix available. See pages 9697 for test mix information. Similar Phases: Rtx-5MS, HP-5MS, PTE-5, CP-Sil 8 CB low bleed/MS, BPX-5, AT-5MS
CH 3 O Si CH 3 CH 3 Si CH 3
m
CH 3 O Si CH 3
n
Structure of poly(dimethylsiloxy)poly(1,4-bis (dimethylsiloxy) phenylene)siloxane
50 ppb Formaldehyde page 380 Amines and Nitriles page 397 Diesel Fuel page 348 EPA Air Analysis Compendium page 379 Method TO-15 (1 ppV Standard) EPA Method 525.2 page 367 EPA Method 551.1 page 377 EPA Method 8061 page 368 (Phthalate Esters) Narcotics page 420 Organochlorine Pesticides (EPA Method 8081A) Organophosphorous Pesticides (EPA Method 8141 A) Phenols Phenols Polyethyleneamines Substituted Anilines Sulfur in Air page 350 page 356 page 369 page 412 page 396 page 411 page 380
GC and GC/MS
DB-5ms
0.18 0.20 0.20 0.20 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.25
40 12 25 50 15 15 15 15 25 25 30 30 30 30
0.18 0.33 0.33 0.33 0.10 0.25 0.50 1.00 0.25 0.40 0.10 0.25 0.50 1.00
-60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350
121-5542 128-5512 128-5522 128-5552 122-5511 122-5512 122-5516 122-5513 122-5522 122-552A 122-5531 122-5532 122-5536 122-5533
243
GC Columns
DB-5ms (continued)
0.25 0.25 0.25 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32
50 60 60 15 15 15 25 30 30 30 30 50 50 60 60 60 60 15 15 15 30 30 30
0.25 0.10 0.25 0.10 0.25 1.00 0.52 0.10 0.25 0.50 1.00 0.25 1.00 0.10 0.25 0.50 1.00 0.50 1.00 1.50 0.50 1.00 1.50
-60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 300/320 -60 to 300/320 -60 to 300/320 -60 to 300/320 -60 to 300/320 -60 to 300/320
122-5552 122-5561 122-5562 123-5511 123-5512 123-5513 123-5526 123-5531 123-5532 123-5536 123-5533 123-5552 123-5553 123-5561 123-5562 123-5566 123-5563 125-5517 125-551J 125-5512 125-5537 125-553J 125-5532
GC and GC/MS
0.32 0.53 0.53 0.53 0.53 0.53 0.53
DB-5ms.ITD
0.25 30 0.25 -60 to 325/350 122-4132
HP-5ms
CH 3 O Si CH 3
95%
C6H5 O Si C6H5
5%
(5%-Phenyl)-methylpolysiloxane Identical selectivity to HP-5 Non-polar Very low bleed characteristics, ideal for GC/MS Excellent inertness for active compounds including acidic and basic compounds Improved signal-to-noise ratio for better sensitivity and mass spectral integrity Bonded and cross-linked Solvent rinsable Equivalent to USP Phase G27 Similar Phases: Rtx-5MS, Rtx-5 Amine, DB-5MS, PTE-5, CP-Sil 8 CB low bleed/MS, BPX-5, AT-5, MDN-5
244
GC Columns
HP-5ms Chromatograms
Chlorinated Pesticides: page 352 EPA Method 508 Nitrogen/Phosphorus Containing page 356 Pesticides: EPA Method 507 Organohalide Pesticides in Water, EPA Method 505 Phenols on HP-5MS USEPA Method 8270 Semivolatile Compounds page 352 page 412 page 366
HP-5ms
0.20 0.20 0.20 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32
12 25 50 15 15 15 30 30 30 30 60 60 15 15 15 25 30 30 30 30 60 60
0.33 0.33 0.33 0.10 0.25 1.00 0.10 0.25 0.50 1.00 0.10 0.25 0.10 0.25 1.00 0.52 0.10 0.25 0.50 1.00 0.10 0.25
-60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350
19091S-101 19091S-102 19091S-105 19091S-331 19091S-431 19091S-231 19091S-333 19091S-433 19091S-133 19091S-233
GC and GC/MS
19091S-336 19091S-436 19091S-311 19091S-411 19091S-211 19091S-112 19091S-313 19091S-413 19091S-113 19091S-213 19091S-316 19091S-416
245
GC Columns
DB-XLB
EXceptionally Low Bleed Low polarity Extended temperature limit of 340/360C Unique selectivity Excellent inertness for active compounds Ideal for confirmational analyses Excellent for pesticides, herbicides, PCBs and PAHs Ideal for GC/MS Ion trap testing and certification available Bonded and cross-linked Solvent rinsable Test mix available. See pages 9697 for test mix information. Similar Phase: MDN-12 Note: DB-XLB is designed for inhibiting column bleed at high temperatures. It also appears to have inadvertently inherited an exceptional ability for separating many PCB congeners when used with MS detection. This stellar performance was maximized after careful optimization of the column dimensions, temperature programs, and carrier gas flow conditions... (Frame, G. Analytical Chemistry News & Features, Aug. 1, 1997, 468A-475A)
GC and GC/MS
DB-XLB Chromatograms
Aroclors 1016-1268 (without 1221) CLP Pesticides PCB Congeners in DIN Method EPA 508.1 Pesticides EPA 552.2 EPA 8151A Phenoxy Acid Herbicides - Methyl Derivatives page 361 page 349 page 362 page 351 page 370 page 357 Garlic Oil Herbicides PCBs by EPA Method 8082 Phenols Ylang Ylang Oil page 384 page 358 page 363 page 369 page 386
246
GC Columns
DB-XLB
0.18 0.18 0.18 0.20 0.20 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.45 0.53 0.53
20 30 60 12 25 15 15 15 30 30 30 30 60 15 15 30 30 30 30 60 30 15 30
0.18 0.18 0.18 0.33 0.33 0.10 0.25 1.00 0.10 0.25 0.50 1.00 0.25 0.25 1.00 0.10 0.25 0.50 1.00 0.25 1.27 1.50 1.50
30 to 340/360 30 to 340/360 30 to 340/360 30 to 340/360 30 to 340/360 30 to 340/360 30 to 340/360 30 to 340/360 30 to 340/360 30 to 340/360 30 to 340/360 30 to 340/360 30 to 340/360 30 to 340/360 30 to 340/360 30 to 340/360 30 to 340/360 30 to 340/360 30 to 340/360 30 to 340/360 30 to 320/340 30 to 320/340 30 to 320/340
121-1222 121-1232 121-1262 128-1212 128-1222 122-1211 122-1212 122-1213 122-1231 122-1232 122-1236 122-1233 122-1262 123-1212 123-1213 123-1231
GC and GC/MS
123-1232 123-1236 123-1233 123-1262 124-1232* 125-1212 125-1232
DB-XLB.ITD
0.25 30 0.25 30 to 340/360 122-4232
247
GC Columns
DB-35
(35%-Phenyl)-methylpolysiloxane Midpolarity slightly more polar than HP-35 Low bleed Inert to active solutes Ideal for confirmational analyses Bonded and cross-linked Solvent rinsable Equivalent to USP Phase G42 Test mix available. See pages 9697 for test mix information. Similar Phases: Rtx-35, SPB-35, AT-35, Sup-Herb, HP-35, BPX-35
DB-35
0.25 0.25 0.25 0.25 0.32 0.32 0.32 0.32 0.53 0.53 0.53 0.53 0.53
30 30 30 60 30 30 30 60 15 30 30 60 60
0.25 0.50 1.00 0.25 0.25 0.50 1.00 0.25 1.00 0.50 1.00 1.00 1.50
40 to 300/320 40 to 300/320 40 to 280/300 40 to 300/320 40 to 300/320 40 to 300/320 40 to 300/320 40 to 300/320 40 to 280/300 40 to 280/300 40 to 280/300 40 to 280/300 40 to 280/300
122-1932 122-1933 122-1934 122-1962 123-1932 123-1933 123-1934 123-1962 125-1912 125-1937 125-1932 125-1962 125-1963
GC and GC/MS
248
GC Columns
HP-35
(35%-Phenyl)-methylpolysiloxane Midpolarity slightly less polar than DB-35 Inert to active solutes Ideal for confirmational analyses Bonded and cross-linked Solvent rinsable Equivalent to USP Phase G42 Similar Phases: Rtx-35, SPB-35, AT-35, Sup-Herb, DB-35, BPX-35
HP-35 Chromatograms
Primary Amines page 396
HP-35
0.25 0.25 0.25 0.32 0.32 0.32
15 30 60 30 30 30
0.25 0.25 0.25 0.15 0.25 0.50
40 to 300/320 40 to 300/320 40 to 300/320 40 to 300/320 40 to 300/320 40 to 300/320
19091G-131 19091G-133 19091G-136 19091G-013 19091G-113
GC and GC/MS
19091G-213
249
GC Columns
DB-35ms
Equivalent to a (35%-Phenyl)-methylpolysiloxane Midpolarity Very low bleed characteristics, ideal for GC/MS Extended temperature limit of 340/360C Excellent inertness for active compounds Ideal for confirmational analyses Bonded and cross-linked Solvent rinsable Replaces HP-35ms Close equivalent to USP Phase G42 Test mix available. See pages 9697 for test mix information. Similar Phases: Rtx-35, SPB-35, AT-35, Sup-Herb, MDN-35, BPX-35
Anilines Barbiturates Benzodiazepines Chlordane CLP Pesticides EPA 508.1 Pesticides EPA 552.2 EPA 8151A Phenoxy Acid Herbicides - Methyl Derivatives page 411 page 418 page 419 page 364 page 349 page 351 page 370 page 357 Organochlorine Pesticides (EPA Method 8081A) Organophosphorous Pesticides (EPA Method 8141 A) PCBs by EPA Method 8082 Toxaphene page 350 page 357 page 363 page 364
GC and GC/MS
DB-35ms
0.20 0.20 0.25 0.25 0.25 0.25 0.32 0.32 0.32 0.53 0.53 0.53
15 25 15 30 30 60 15 30 60 15 30 30
0.33 0.33 0.25 0.15 0.25 0.25 0.25 0.25 0.25 1.00 0.50 1.00
128-3812 128-3822 122-3812 122-3831 122-3832 122-3862 123-3812 123-3832 123-3862 125-3812 125-3837 125-3832
250
GC Columns
DB-17
(50%-Phenyl)-methylpolysiloxane Midpolarity slightly more polar than HP-50+ Excellent for confirmational analyses Bonded and cross-linked Solvent rinsable Equivalent to USP Phase G3 Test mix available. See pages 9697 for test mix information. Similar Phases: HP-50+, Rtx-50, CP-Sil 24 CB, 007-17(MPS-50), HP-17, SP-2250, SPB-50, ZB-50, AT-50
CH 3 O Si CH 3
50%
C6H5 O Si C6H5
50%
DB-17 Chromatograms
Common Drug Screen Free Steroids page 415 page 421 Triazine Herbicides page 359
DB-17
0.05 0.10 0.10 0.10 0.10 0.18 0.18 0.18 0.18 0.20 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.32 0.32 0.32 0.32
10 10 10 20 20 10 10 20 20 25 15 15 15 30 30 30 60 60 15 15 15 30
0.10 0.10 0.20 0.10 0.20 0.18 0.30 0.18 0.30 0.16 0.15 0.25 0.50 0.15 0.25 0.50 0.25 0.50 0.15 0.25 0.50 0.15
40 to 280/300 40 to 280/300 40 to 280/300 40 to 280/300 40 to 280/300 40 to 280/300 40 to 280/300 40 to 280/300 40 to 280/300 40 to 280/300 40 to 280/300 40 to 280/300 40 to 280/300 40 to 280/300 40 to 280/300 40 to 280/300 40 to 280/300 40 to 280/300 40 to 280/300 40 to 280/300 40 to 280/300 40 to 280/300
126-1713 127-1712 127-1713
GC and GC/MS
127-1722 127-1723 121-1712 121-1713 121-1722 121-1723 128-1721 122-1711 122-1712 122-1713 122-1731 122-1732 122-1733 122-1762 122-1763 123-1711 123-1712 123-1713 123-1731
251
GC Columns
DB-17 (continued)
0.32 0.32 0.32 0.32 0.45 0.45 0.53 0.53 0.53 0.53 0.53 0.53 0.53 0.53 0.53 0.53
30 30 60 60 15 30 5 15 15 15 15 15 30 30 30 30 60 60
0.25 0.50 0.25 0.50 0.85 0.85 2.00 0.10 0.25 0.50 1.00 1.50 0.25 0.50 1.00 1.50 1.00 1.50
40 to 280/300 40 to 280/300 40 to 280/300 40 to 280/300 40 to 260/280 40 to 260/280 40 to 260/280 40 to 260/280 40 to 260/280 40 to 280/300 40 to 260/280 40 to 260/280 40 to 260/280 40 to 260/280 40 to 260/280 40 to 260/280 40 to 260/280 40 to 260/280
123-1732 123-1733 123-1762 123-1763 124-1712* 124-1732* 125-1704 125-1719 125-1711 125-1717 125-1712 125-1713 125-1731 125-1737 125-1732 125-1733 125-1762 125-1763
GC and GC/MS
0.53 0.53
HP-50 +
(50%-Phenyl)-methylpolysiloxane Midpolarity-slightly less polar than DB-17 Excellent for confirmational analyses Bonded and cross-linked Solvent rinsable Equivalent to USP Phase G3 Similar Phases: DB-17, Rtx-50, CP-Sil 24 CB, 007-17(MPS-50), SP-2250, SPB-50, ZB-50, AT-50
HP-50+
0.20 0.20 0.20 0.20 0.20 0.25 0.25 0.25 0.25 0.25
12 12 25 25 50 15 15 15 30 30
0.16 0.31 0.16 0.31 0.31 0.15 0.25 0.50 0.15 0.25
19091L-001 19091L-101 19091L-002 19091L-102 19091L-105 19091L-331 19091L-431 19091L-131 19091L-333 19091L-433
252
GC Columns
HP-50+ (continued)
0.25 0.25 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.53 0.53 0.53
30 60 15 15 30 30 30 60 60 15 30 30
0.50 0.25 0.25 0.50 0.15 0.25 0.50 0.25 0.50 1.00 0.50 1.00
19091L-133 19091L-436 19091L-411 19091L-111 19091L-313 19091L-413 19091L-113 19091L-416 19091L-116 19095L-021 19095L-523 19095L-023
DB-17ms
Virtually equivalent to (50%-Phenyl)-methylpolysiloxane 320/340C upper temperature limit Very low bleed midpolarity column, ideal for GC/MS Excellent inertness for active compunds Enhanced mass spectral integrity Bonded and cross-linked Solvent rinsable Best column for CLP pesticides Test mix available. See pages 9697 for test mix information. Similar Phases: HP-50+, Rtx-50, 007-17, SP-2250, SPB-50, BPX-50, SPB-17, AT-50
GC and GC/MS
Hallucinogens PAHs page 420 page 368 Tocopherols page 419
DB-17ms
0.18 0.25 0.25 0.25 0.25 0.25 0.32 0.32 0.32 0.32
20 15 15 30 30 60 15 15 30 30
0.18 0.15 0.25 0.15 0.25 0.25 0.15 0.25 0.15 0.25
121-4722 122-4711 122-4712 122-4731 122-4732 122-4762 123-4711 123-4712 123-4731 123-4732
253
GC Columns
DB-23
CN CH 2 CH 2 CH 2 O Si CH 3 n
(50%-Cyanopropyl)-methylpolysiloxane High polarity Designed for separation of fatty acid methyl esters (FAMEs) Excellent resolution for cis- and trans-isomers Bonded and cross-linked Solvent rinsable Replaces HP-23 Close equivalent to USP Phase G5 Test mix available. See pages 9697 for test mix information. Similar Phases: SP-2330, Rtx-2330, 007-23, AT-Silar, CP-Sil 88, BPX-70, SP-2340
Structure of Cyanopropylmethylpolysiloxane
DB-23 Chromatograms
Canola Oil Margarine Partially Hydrogenated FAMEs page 393 FAMEs page 391
DB-23
0.18 0.25 0.25 0.25 0.25 0.25 0.32 0.32 0.32 0.32 0.32 0.53 0.53
20 15 30 30 60 60 15 30 30 60 60 15 30
0.20 0.25 0.15 0.25 0.15 0.25 0.25 0.15 0.25 0.15 0.25 0.50 0.50
40 to 250/260 40 to 250/260 40 to 250/260 40 to 250/260 40 to 250/260 40 to 250/260 40 to 250/260 40 to 250/260 40 to 250/260 40 to 250/260 40 to 250/260 40 to 230/240 40 to 230/240
121-2323 122-2312 122-2331 122-2332 122-2361 122-2362 123-2312 123-2331 123-2332 123-2361 123-2362 125-2312 125-2332
GC and GC/MS
DB-200
CF 3 CH 2 CH 2 O Si CH 3 n
(35% Trifluoropropyl)-methylpolysiloxane 300/320C temperature limit Midpolarity (more polar than DB-1701 or DB-17) Ideal for difficult to separate positional isomers Unique interactions with compounds containing nitro, halogen and carbonyl groups Low ECD bleed Unique selectivity Close equivalent to USP Phase G6 Test mix available. See pages 9697 for test mix information. Similar Phases: Rtx-200
Structure of Trifluoropropylmethylpolysiloxane
254
GC Columns
DB-200 Chromatograms
Acrylate Impurities Aromatic Solvents page 410 page 407 Solvents page 408
DB-200
0.18 0.25 0.25 0.25 0.25 0.32 0.32 0.45 0.53 0.53 0.53
20 15 15 30 30 30 30 30 15 30 30
0.40 0.25 0.50 0.25 0.50 0.25 0.50 0.85 1.00 0.50 1.00
30 to 300/320 30 to 300/320 30 to 300/320 30 to 300/320 30 to 300/320 30 to 300/320 30 to 300/320 30 to 280/300 30 to 280/300 30 to 280/300 30 to 280/300
121-2023 122-2012 122-2013 122-2032 122-2033 123-2032 123-2033 124-2032* 125-2012 125-2037 125-2032
DB-210
(50%-Trifluoropropyl)-methylpolysiloxane High polarity Excellent for U.S. EPA Methods 8140 and 609 Bonded and cross-linked Solvent rinsable Exact replacement of HP-210 Close equivalent to USP Phase G6 Test mix available. See pages 9697 for test mix information. Similar Phases: SP-2401
CF3 CH 2 CH 2 O Si CH 3 n
GC and GC/MS
Structure of Trifluoropropylmethylpolysiloxane
DB-210
0.18 0.25 0.25 0.25 0.32 0.32 0.32 0.32 0.32 0.53 0.53
20 15 30 30 15 15 30 30 30 15 30
0.30 0.25 0.25 0.50 0.25 0.50 0.15 0.25 0.50 1.00 1.00
121-0223 122-0212 122-0232 122-0233 123-0212 123-0213 123-0231 123-0232 123-0233 125-0212 125-0232
255
GC Columns
DB-225
CN CH 2 CH 2 CH 3 O Si CH 3
50%
CH 2 O Si C6H5
50%
(50%-Cyanopropylphenyl)-dimethylpolysiloxane Mid/high polarity Excellent for separations of cis- and trans-fatty acid methyl esters (FAMEs) Bonded and cross-linked Solvent rinsable Exact replacement of HP-225 Close equivalent to USP Phase G7 Test mix available. See pages 9697 for test mix information. Similar Phases: SP-2330, CP-Sil 43 CB, Rtx-225, BP-225, OV-225, 007-225, AT-225
Structure of Cyanopropylphenylmethylpolysiloxane
Alditol Acetates FAME Standard page 388 page 392 Tetrachlorodibenzo-p-furans page 359
DB-225
0.10 0.18 0.25
20 20 15 30 30 15 30 30 15 30 30
0.10 0.20 0.25 0.15 0.25 0.25 0.15 0.25 1.00 0.50 1.00
127-2222 121-2223 122-2212 122-2231 122-2232 123-2212 123-2231 123-2232 125-2212 125-2237 125-2232
GC and GC/MS
0.25 0.25 0.32 0.32 0.32 0.53 0.53 0.53
DB-225ms
Virtually equivalent to (50%-Cyanopropylphenyl)-methylpolysiloxane Mid/high polarity Excellent for separations of cis- and trans-fatty acid methyl esters (FAMEs) Low bleed Bonded and cross-linked Solvent rinsable Close equivalent to USP Phase G7 Similar Phases: HP-225, SP-2330, CP-Sil 43 CB, Rtx-225, BP-225, OV-225, 007-225, AT-225
Tetrachlorodibenzo-p-furans page 359
256
GC Columns
DB-225ms
0.25 0.25 0.25 0.32 0.32
15 30 60 15 30
0.25 0.25 0.25 0.25 0.25
40 to 240 40 to 240 40 to 240 40 to 240 40 to 240
122-2912 122-2932 122-2962 123-2912 123-2932
DB-624
Specifically designed for the analysis of volatile priority pollutants No cryogenics needed for U.S. EPA Method 502.2 Excellent for U.S. EPA Methods: 501.3, 502.2, 503.1, 524.2, 601, 602, 8010, 8015, 8020, 8240, 8260 Excellent inertness for active compounds Bonded and cross-linked Solvent rinsable Exact replacement of HP-624 Equivalent to USP Phase G43 Test mix available. See pages 9697 for test mix information. Similar Phases: AT-624, Rtx-624, PE-624, 007-624, 007-502, CP-624, ZB-624
GC and GC/MS
1,3-Butadiene Alcohols EPA Method 504 by GC/MS EPA Volatiles GC/MS EPA Method 8021 Esters page 428 page 394 page 376 page 371 page 373 page 402 Ethers Fast VOA Analysis Glycols Halogenated Hydrocarbon Nitrogen Based Solvents Residual Solvents, USP <467> page 403 page 375 page 403 page 405 page 409 page 424
DB-624
0.18 0.20 0.25 0.25 0.32 0.32 0.45 0.45 0.53 0.53 0.53
20 25 30 60 30 60 30 75 30 60 75
1.00 1.12 1.40 1.40 1.80 1.80 2.55 2.55 3.00 3.00 3.00
-20 to 260 -20 to 260 -20 to 260 -20 to 260 -20 to 260 -20 to 260 -20 to 260 -20 to 260 -20 to 260 -20 to 260 -20 to 260
121-1324 128-1324 122-1334 122-1364 123-1334 123-1364 124-1334* 124-1374* 125-1334 125-1364 125-1374
257
GC Columns
DB-1301 and DB-1701

DB-1301: (6%-Cyanopropyl-phenyl) methylpolysiloxane DB-1301: Equivalent to USP Phase G43 DB-1701: (14%-Cyanopropyl-phenyl)-methylpolysiloxane Low/midpolarity Bonded and cross-linked Exact replacement of HP-1301 and HP-1701 Solvent rinsable Test mix available. See pages 9697 for test mix information. Similar Phases to DB-1301: Rtx-1301, PE-1301 Similar Phases to DB-1701: SPB-1701, CP-Sil 19 CB, Rtx-1701, BP-10, OV-1701, 007-1701, ZB-1701
DB-1301
0.18 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.32 0.32 0.32 0.32 0.32 0.32 0.53 0.53 0.53 0.53 0.53
10 15 15 30 30 30 60 60 15 15 30 30 60 60 15 15 30 30 60
0.40 0.25 1.00 0.25 0.50 1.00 0.25 1.00 0.25 1.00 0.25 1.00 0.25 1.00 1.00 1.50 1.00 1.50 1.50
-20 to 280/300 -20 to 280/300 -20 to 280/300 -20 to 280/300 -20 to 280/300 -20 to 280/300 -20 to 280/300 -20 to 280/300 -20 to 280/300 -20 to 280/300 -20 to 280/300 -20 to 280/300 -20 to 280/300 -20 to 280/300 -20 to 260/280 -20 to 260/280 -20 to 260/280 -20 to 260/280 -20 to 260/280
121-1313 122-1312 122-1313 122-1332 122-1336 122-1333 122-1362 122-1363 123-1312 123-1313 123-1332 123-1333 123-1362 123-1363 125-1312 125-1313 125-1332 125-1333 125-1363
GC and GC/MS
Acrylate Impurities Fentanyls Organochlorine Pesticides page 410 page 419 page 354 Phenoxy Acid Herbicides TMS Sugars Triazine Herbicides page 358 page 389 page 359
258
GC Columns
DB-1701
0.10 0.10 0.18 0.18 0.20 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.45 0.45 0.53 0.53 0.53 0.53 0.53 0.53 0.53 0.53 0.53 0.53
20 20 10 20 25 15 15 15 30 30 30 60 60 60 60 15 15 15 30 30 30 30 50 60 60 60 30 30 15 15 15 30 30 30 30 30 60 60
0.10 0.40 0.40 0.40 0.20 0.15 0.25 1.00 0.15 0.25 1.00 0.15 0.25 0.50 1.00 0.15 0.25 1.00 0.10 0.15 0.25 1.00 1.00 0.15 0.25 1.00 0.42 0.85 0.25 0.50 1.00 0.10 0.25 0.50 1.00 1.50 1.00 1.50
-20 to 280/300 -20 to 280/300 -20 to 280/300 -20 to 280/300 -20 to 280/300 -20 to 280/300 -20 to 280/300 -20 to 280/300 -20 to 280/300 -20 to 280/300 -20 to 280/300 -20 to 280/300 -20 to 280/300 -20 to 280/300 -20 to 280/300 -20 to 280/300 -20 to 280/300 -20 to 280/300 -20 to 280/300 -20 to 280/300 -20 to 280/300 -20 to 280/300 -20 to 280/300 -20 to 280/300 -20 to 280/300 -20 to 280/300 -20 to 260/280 -20 to 260/280 -20 to 260/280 -20 to 260/280 -20 to 260/280 -20 to 260/280 -20 to 260/280 -20 to 260/280 -20 to 260/280 -20 to 260/280 -20 to 260/280 -20 to 260/280
127-0722 127-0723 121-0713 121-0723 128-0722 122-0711 122-0712 122-0713 122-0731 122-0732 122-0733 122-0761 122-0762 122-0766 122-0763 123-0711
GC and GC/MS
123-0712 123-0713 123-0739 123-0731 123-0732 123-0733 123-0753 123-0761 123-0762 123-0763 124-0737* 124-0732* 125-0711 125-0717 125-0712 125-0739 125-0731 125-0737 125-0732 125-0733 125-0762 125-0763
259
GC Columns
PEG Columns
H -HO C H
H C H O n H
Polyethylene Glycol (PEG) Columns

Agilent offers a full range of PEG columns. Even though each phase is based on the polyethylene glycol polymer, strict control of the cross-linking and deactivation processes results in a variety of unique phase characteristics to meet the varying analysis needs of your laboratory.
PEG Column Features Lowest operating temperature limit Most similar to Carbowax 20M Available in 0.10 mm id Highly inert Benefits Analyze low boiling point analytes Transfer older methods to bonded phase Used for Fast GC for high sample throughput Broad analyte compatibility Compromise for high and low boiling analytes Analyze high boiling compounds Excellent general purpose column Best choice for MS use Broad analyte compatibility Can inject organic acids without derivitization Good peak shape for basic compounds *Cannot be solvent rinsed
Structure of Polyethylene Glycol
DB-WAX
DB-WAXetr HP-INNOWax
Middle operating temperature range Highest upper temperature limit Wide chemical compatibility Lowest bleed at elevated temperatures Highly inert Acid modified
DB-FFAP, HP-FFAP
GC and GC/MS
CAM (see page 296) Base modified *Non-bonded
DB-WAX
Polyethylene glycol (PEG) Close equivalent to USP Phase G16 High polarity Lower temperature limit of 20C is lowest of any bonded PEG phase; improves resolution of low boiling point analytes Column-to-column reproducibility Bonded and cross-linked Exact replacement of HP-WAX Solvent rinsable Test mix available. See pages 9697 for test mix information. Similar Phases: HP-20M, SUPELCOWAX 10, CP-WAX 52 CB, SUPEROX II, CB-WAX, Stabilwax, BP-20, 007-CW, Carbowax, HP-INNOWax, Rtx-WAX, ZB-WAX
260
GC Columns
PEG Columns
DB-WAX Chromatograms
Aldehydes and Ketones Aromatics Ethylene Oxide FAME Standard Fast Styrene Analysis Formaldehyde-Underivatized page 398 page 400 page 414 page 392 page 436 page 399 Fragrance Reference Standard Glycols Peppermint Oil Phenols Spearmint Oil (Western) page 382 page 403 page 385 page 413 page 386
DB-WAX
0.05 0.05 0.10 0.10 0.10 0.10 0.18 0.18 0.18 0.18 0.18 0.20 0.20 0.20 0.20 0.20 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.25
10 10 10 10 20 20 10 10 20 20 40 25 25 50 50 60 15 15 15 30 30 30 60 60 60
0.05 0.10 0.10 0.20 0.10 0.20 0.18 0.30 0.18 0.30 0.30 0.20 0.20 0.20 0.40 0.20 0.15 0.25 0.50 0.15 0.25 0.50 0.15 0.25 0.50
20 to 250/260 20 to 240/250 20 to 250/260 20 to 240/250 20 to 250/260 20 to 240/250 20 to 250/260 20 to 240/250 20 to 250/260 20 to 240/250 20 to 240/250 20 to 250/260 20 to 250/260 20 to 250/260 20 to 240/250 20 to 250/260 20 to 250/260 20 to 250/260 20 to 240/250 20 to 250/260 20 to 250/260 20 to 240/250 20 to 250/260 20 to 250/260 20 to 240/250
126-7012 126-7013 127-7012 127-7013 127-7022 127-7023 121-7012 121-7013 121-7022 121-7023 121-7043 128-7022 128-7032 128-7052 128-7053 128-7062 122-7011 122-7012 122-7013 122-7031 122-7032 122-7033 122-7061 122-7062 122-7063
GC and GC/MS
261
GC Columns
PEG Columns
DB-WAX (continued)
0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.45 0.45 0.45 0.53 0.53 0.53 0.53 0.53
15 15 15 30 30 30 60 60 15 30 60 15 15 30 30 30 60
0.15 0.25 0.50 0.15 0.25 0.50 0.25 0.50 0.85 0.85 0.85 0.50 1.00 0.25 0.50 1.00 1.00
20 to 250/260 20 to 250/260 20 to 240/250 20 to 250/260 20 to 250/260 20 to 240/250 20 to 250/260 20 to 240/250 20 to 230/240 20 to 230/240 20 to 230/240 20 to 230/240 20 to 230/240 20 to 230/240 20 to 230/240 20 to 230/240 20 to 230/240
123-7011 123-7012 123-7013 123-7031 123-7032 123-7033 123-7062 123-7063 124-7012* 124-7032* 124-7062* 125-7017 125-7012 125-7031 125-7037 125-7032 125-7062
GC and GC/MS
0.53
DB-WAXetr
Polyethylene glycol (PEG) Extended Temperature Range (etr) High polarity Excellent column-to-column repeatability Bonded and cross-linked Solvent rinsable Close equivalent to USP Phase G16 Test mix available. See pages 9697 for test mix information. Similar Phases: HP-20M, SUPELCOWAX 10, CP-WAX 52 CB, SUPEROX II, CB-WAX, Stabilwax, BP-20, 007-CW, Carbowax, HP-INNOWax, ZB-WAX
262
GC Columns
PEG Columns
DB-WAXetr Chromatograms
Alcohols Impurities in Mixed Xylenes Impurities in Styrene page 394 page 401 page 401 Organic Acids Solvents Solvents page 395 page 406 page 407
DB-WAXetr
0.20 0.25 0.25 0.25 0.25 0.25 0.25 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.45 0.53 0.53 0.53 0.53 0.53 0.53 0.53 0.53
25 15 15 30 30 60 60 15 15 15 30 30 30 50 60 60 60 5 5 15 15 30 30 30 60 60
0.40 0.25 0.50 0.25 0.50 0.25 0.50 0.25 0.50 1.00 0.25 0.50 1.00 1.00 0.25 0.50 1.00 1.70 2.00 1.00 2.00 1.00 1.50 2.00 1.00 1.50
30 to 250/260 30 to 260/280 30 to 250/260 30 to 260/280 30 to 250/260 30 to 260/280 30 to 250/260 30 to 260/280 30 to 250/260 30 to 250/260 30 to 260/280 30 to 250/260 30 to 250/260 30 to 250/260 30 to 260/280 30 to 250/260 30 to 250/260 50 to 230/250 50 to 230/250 30 to 240/260 50 to 230/250 30 to 240/260 30 to 230/240 50 to 230/250 30 to 240/260 30 to 230/240
128-7323 122-7312 122-7313 122-7332 122-7333 122-7362 122-7363 123-7312 123-7313 123-7314 123-7332 123-7333
GC and GC/MS
123-7334 123-7354 123-7362 123-7363 123-7364 124-7304* 125-7304 125-7312 125-7314 125-7332 125-7333 125-7334 125-7362 125-7363
263
GC Columns
PEG Columns
HP-INNOWax
Polyethylene glycol (PEG) High polarity Highest upper temperature limits of the bonded PEG phases Column-to-column repeatability Bonded and cross-linked Solvent rinsable Close equivalent to USP Phase G16 Similar Phases: HP-20M, SUPELCOWAX 10, CP-WAX 52 CB, SUPEROX II, CB-WAX, Stabilwax, BP-20, 007-CW, Carbowax, DB-WAXetr, ZB-WAX, DB-WAXetr
HP-INNOWax Chromatograms
Alcohols Aldehydes and Acids Aromatics Anlalysis-Ethylbenzene Impurities ASTM D16 Aromatics Analysis Bourbon Chlorinated Isooctane Esters Free Fatty Acids Free Organic Acids/C4-C5 Isomers page 395 page 397 page 435 page 435 page 388 page 406 page 402 page 389 page 395 Impurities in Ethylbenzene Impurities in p-Xylene-ASTM D3798 Monomers in Latex Paint by Headspace Perfume Polyunsaturated FAMEs Rapid Analysis of Water in Organic Solvents Strawberry Syrup Sulfur and Selenium in Garlic by Headspace page 401 page 435 page 410 page 383 page 391 page 409 page 388 page 389
GC and GC/MS
HP-INNOWax
0.20 0.20 0.20 0.20 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.32 0.32 0.32 0.32 0.32 0.32
25 25 50 50 15 15 30 30 30 60 60 60 15 15 30 30 30 60
0.20 0.40 0.20 0.40 0.25 0.50 0.15 0.25 0.50 0.15 0.25 0.50 0.25 0.50 0.15 0.25 0.50 0.15
40 to 260/270 40 to 260/270 40 to 260/270 40 to 260/270 40 to 260/270 40 to 260/270 40 to 260/270 40 to 260/270 40 to 260/270 40 to 260/270 40 to 260/270 40 to 260/270 40 to 260/270 40 to 260/270 40 to 260/270 40 to 260/270 40 to 260/270 40 to 260/270
19091N-102 19091N-202 19091N-105 19091N-205 19091N-131 19091N-231 19091N-033 19091N-133 19091N-233 19091N-036 19091N-136 19091N-236 19091N-111 19091N-211 19091N-013 19091N-113 19091N-213 19091N-016
264
GC Columns
PEG Columns
HP-INNOWax (continued)
0.32 0.32 0.53 0.53 0.53
60 60 15 30 60
0.25 0.50 1.00 1.00 1.00
40 to 260/270 40 to 260/270 40 to 240/250 40 to 240/250 40 to 240/250
19091N-116 19091N-216 19095N-121 19095N-123 19095N-126
DB-FFAP
Nitroterephthalic acid modified polyethylene glycol High polarity Temperature range from 40 to 250C Designed for the analysis of volatile fatty acids and phenols Replaces OV-351 Bonded and cross-linked Solvent rinsable Close equivalent to USP Phase G35 We do not recommend the use of water or methanol to rinse DB-FFAP GC columns. Test mix available. See pages 9697 for test mix information. Similar Phases: Stabilwax-DA, HP-FFAP, Nukol, 007-FFAP, BP21, CP-Wax 58 (FFAP) CB, AT-1000, OV-351, CP-FFAP-CB
GC and GC/MS
DB-FFAP Chromatograms
Aspirin and Ibuprofen in Methanol page 421 Organic Acids page 390
DB-FFAP
0.20 0.25 0.25 0.25 0.25 0.25 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32
25 15 30 30 60 60 15 15 25 30 30 30 50 60 60 60
0.30 0.25 0.25 0.50 0.25 0.50 0.25 1.00 0.50 0.25 0.50 1.00 0.50 0.25 0.50 1.00
40 to 250 40 to 250 40 to 250 40 to 250 40 to 250 40 to 250 40 to 250 40 to 250 40 to 250 40 to 250 40 to 250 40 to 250 40 to 250 40 to 250 40 to 250 40 to 250
128-3222 122-3212 122-3232 122-3233 122-3262 122-3263 123-3212 123-3214 123-3223 123-3232 123-3233 123-3234 123-3253 123-3262 123-3263 123-3264
265
GC Columns
PEG Columns
DB-FFAP (continued)
0.45 0.45 0.53 0.53 0.53 0.53 0.53 0.53 0.53 0.53
15 30 10 15 15 30 30 30 30 60
0.85 0.85 1.00 0.50 1.00 0.25 0.50 1.00 1.50 1.00
40 to 250 40 to 250 40 to 250 40 to 250 40 to 250 40 to 250 40 to 250 40 to 250 40 to 250 40 to 250
124-3212* 124-3232* 125-32H2 125-3217 125-3212 125-3231 125-3237 125-3232 125-3233 125-3262
HP-FFAP
Nitroterephthalic acid modified polyethylene glycol High polarity Temperature range from 60 to 240/250C (230/240C for 0.53 mm) Designed for the analysis of volatile fatty acids and phenols Replaces OV-351 Bonded and cross-linked Solvent rinsable Close equivalent to USP Phase G35 We do not recommend the use of water or methanol to rinse HP-FFAP GC columns. Similar Phases: Stabilwax-DA, DB-FFAP, Nukol, 007-FFAP, BP21, CP-WAX 58 (FFAP) CB, AT-1000, OV-351, CP-FFAP-CB
GC and GC/MS
HP-FFAP Chromatograms
Acrylates Alcohol Beverage Standard page 410 page 388 Ethoxyethanol page 395
HP-FFAP
0.20 0.20 0.25 0.25 0.32 0.32 0.32 0.32 0.53 0.53 0.53
25 50 15 30 15 25 30 50 10 15 30
0.30 0.30 0.25 0.25 0.25 0.50 0.25 0.50 1.00 1.00 1.00
60 to 240/250 60 to 240/250 60 to 240/250 60 to 240/250 60 to 240/250 60 to 240/250 60 to 240/250 60 to 240/250 60 to 240 60 to 240 60 to 240
19091F-102 19091F-105 19091F-431 19091F-433 19091F-411 19091F-112 19091F-413 19091F-115 19095F-121 19095F-120 19095F-123 www.agilent.com/chem
266
GC Columns
Chiral Columns
Application Specific Stationary Phases

Agilents chemists have developed many columns with unique characteristics designed to solve the most difficult separation problems of a given method. As a result, we offer a comprehensive line of specialty columns for a variety of applications to enhance the standard phase portfolio. From columns for volatiles to pesticides to petrochemical and more Agilent exceeds standard QA/QC procedures for the manufacturing and testing of all of our specialty columns to ensure they meet the stringent demands for their application. These columns offer reliable, accurate results with the shortest run times possible on complex sample lists and matrices.
Chiral Columns
Our proven Cyclodex-B, CycloSil-B and HP-Chiral B offer the chiral analyst a broad range of chiral separations. Although no single column resolves every enantiomeric pair, our no-hassle return policy allows you to try the column for your application and if it doesnt work, simply return it. Recommendations for choosing a chiral column: Contact Technical Support through your local Agilent office for a more specific recommendation Refer to existing applications and literature Choose CycloSil-B as a general purpose column Use HP-Chiral B when using a nitrogen specific detector
GC and GC/MS
CycloSil-B
30% heptakis (2,3-di-O-methyl-6-O-t-butyl dimethylsilyl)--cyclodextrin in DB-1701 Chiral separations without chiral specific derivatization New stationary phase for improved resolution of many chiral separations Ideal for many chiral -lactones and terpenes
Test mix available. See pages 9697 for test mix information. Because CycloSil-B GC columns are not bonded or cross-linked, we do not recommend solvent rinsing.
Similar Phases: LIPODEX C, Rt- DEXm, -DEX 110, -DEX 120
CycloSil-B Chromatograms
Citrus Flavored Carbonated Beverage (Soda) page 387 Rosemary Oil page 387
30% Heptakis (2,3-di-O-methyl-6-O-t-butyldimethylsilyl)-b-cyclodextrin
CycloSil-B
0.25 0.32
30 30
0.25 0.25
35 to 260/280 35 to 260/280
112-6632 113-6632
267
GC Columns
Chiral Columns
Cyclodex-B
10.5% Beta-Cyclodextrin in DB-1701 Chiral separations without chiral specific derivatization Broad range of resolving potential Excellent peak shape Test mix available. See pages 9697 for test mix information. Because Cyclodex-B GC columns are not bonded or cross-linked, we do not recommend solvent rinsing. Similar Phases: LIPODEX C, Rt- DEXm, -DEX 110, -DEX 120
Cyclodex-B Chromatograms
Menthol page 383
Cyclodex-B
0.25 0.25 0.32 0.53
30 60 30 30
0.25 0.25 0.25 0.50
50 to 230/250 50 to 230/250 50 to 230/250 50 to 210/230
112-2532 112-2562 113-2532 115-2537
GC and GC/MS
HP-Chiral
Beta-cyclodextrin in (35%-Phenyl)-methylpolysiloxane Chiral separations without chiral specific derivatization Phenyl-based polymer provides low bleed and does not interfere with nitrogen-specific detectors. Available in two concentrations of beta-cyclodextrin: 10% and 20% 20% beta-cyclodextrin best choice for initial screening Similar Phases: LIPODEX C, Rt-DEXm, -DEX 110, -DEX 120
HP-Chiral-10
0.25 0.32
30 30
0.25 0.25
30 to 240/250 30 to 240/250
19091G-B133 19091G-B113
HP-Chiral-20
0.25 0.32
30 30
0.25 0.25
30 to 240/250 30 to 240/250
19091G-B233 19091G-B213
268
GC Columns
Food and Flavors
Food and Flavors

Food and flavor analyses place stringent demands on capillary columns. Samples have many components that are difficult to resolve and column-to-column reproducibility becomes critical. J&W Scientific GC columns are ideal for meeting these needs. Our rigorous quality control specifications and extensive QC testing ensure that the column you buy today will perform just like the column you buy tomorrow. Recommended Columns for Food and Flavors DB-23 for FAMEs and cis- and trans-isomers (page 391) DB-XLB and DB-17ht for triglycerides (page 393) DB-FFAP for organic acids (page 390) DB-1, DB-WAX for fragrance compounds (pages 381382) Microbore (0.1 mm id) DB-1, DB-5 or DB-Wax for fast analysis of fragrances and FAMEs (page 392)
Ultra 1 and Ultra 2

Non-polar Ultra 1: 100% Dimethylpolysiloxane Ultra 2: (5%-Phenyl)-methylpolysiloxane HP-1 and HP-5 with tighter specifications for retention index and capacity factors Intended for use with Sadtler Retention Index Library Four volume reference set that identifies compounds by retention indices alone Catalogs over 2000 compounds by their retention behavior on three HP columns Bonded and cross-linked Solvent rinsable Similar Phases: Ultra1: DB-1, HP-1, SPB-1, CP-Sil 5 CB, Rtx-1, BP-1, 007-1(MS) Ultra 2: DB-5, HP-5, SPB-5, CP-Sil 8 CB, Rtx-5, BP-5, CB-5, 007-5, 2B-5
GC and GC/MS
Ultra 1 and Ultra 2 Chromatograms

Ethylene Glycol Mixture Antiepileptic Drugs Flavor Mixture page 404 page 418 page 383 Tricyclic Antipsychotics Urine Drug Screen page 418 page 416
HP-Ultra 1
0.20 0.20 0.20
12 25 25
0.33 0.11 0.33
-60 to 325/350 -60 to 325/350 -60 to 325/350
19091A-101 19091A-002 19091A-102
269
GC Columns
Food and Flavors/High Temperature Columns
HP-Ultra 1 (continued)
0.20 0.20 0.32 0.32 0.32 0.32
50 50 25 25 50 50
0.11 0.33 0.17 0.52 0.17 0.52
-60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350
19091A-005 19091A-105 19091A-012 19091A-112 19091A-015 19091A-115
HP-Ultra 2
0.20 0.20 0.20 0.20 0.20 0.32 0.32 0.32 0.32
12 25 25 50 50 25 25 50 50
0.33 0.11 0.33 0.11 0.33 0.17 0.52 0.17 0.52
-60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350
19091B-101 19091B-002 19091B-102 19091B-005 19091B-105 19091B-012 19091B-112 19091B-015 19091B-115
GC and GC/MS
High Temperature Columns DB-1ht

100% Dimethylpolysiloxane Non-polar Specially processed for extended temperature limit of 400C High temperature, polyimide-coated, fused silica tubing Excellent peak shape and faster elution times for high boilers Bonded and cross-linked Solvent rinsable Test mix available. See pages 9697 for test mix information. Similar Phase: None
DB-1ht
0.25 0.25 0.32 0.32
15 30 15 30
0.10 0.10 0.10 0.10
-60 to 400 -60 to 400 -60 to 400 -60 to 400
122-1111 122-1131 123-1111 123-1131
270
GC Columns
High Temperature Columns
DB-5ht
(5%-Phenyl)-methylpolysiloxane Non-polar Specially processed for extended temperature limit of 400C High temperature, polyimide-coated, fused silica tubing Excellent peak shape and faster elution times for high boilers Bonded and cross-linked Solvent rinsable Test mix available. See pages 9697 for test mix information. Similar Phase: HT5
DB-5ht Chromatograms
Butter Triglycerides page 393
DB-5ht
0.25 0.25 0.32 0.32
15 30 15 30
0.10 0.10 0.10 0.10
-60 to 400 -60 to 400 -60 to 400 -60 to 400
122-5711 122-5731 123-5711 123-5731
GC and GC/MS
DB-17ht
(50%-Phenyl)-methylpolysiloxane Midpolarity Extended upper temperature limit of 365C High temperature, polyimide-coated, fused silica tubing Excellent peak shape and faster elution times for high boilers Improved resolution for triglycerides Ideal for confirmational analyses Bonded and cross-linked Solvent rinsable Test mix available. See pages 9697 for test mix information. Similar Phases: Rtx-65TG, BPX50, CP-TAP CB
DB-17ht Chromatograms
Butter Triglycerides page 393
DB-17ht
0.25 0.25 0.32 0.32 0.32
15 30 15 30 60
0.15 0.15 0.15 0.15 0.15
40 to 340/365 40 to 340/365 40 to 340/365 40 to 340/365 40 to 340/365
122-1811 122-1831 123-1811 123-1831 123-1861
271
GC Columns
Life Sciences
Life Sciences
The life sciences offer some difficult challenges to capillary chromatographers. These include complex sample matrices, the necessity for low level detection and the chemically active characteristics of many of the samples. In response to this, Agilent offers a line of columns which are designed specifically for drugs of abuse testing. Recommended Columns for Life Sciences DB-ALC1 and DB-ALC2 for U.S. Blood Alcohol analysis (page 422) DB-ALC2 and HP-Blood Alcohol column for European Blood Alcohol analysis Low-bleed columns for controlled substances DB-35ms for barbiturates (page 418) DB-17ms for hallucinogens (page 420) DB-EVDX for analysis of drugs of abuse DB-624, DB-1, DB-WAX or HP-INNOWax for Residual Solvent analysis (page 424) See pages 341344 for solvent retention data on the DB-624, DB-1 and DB-WAX
DB-ALC1 and DB-ALC2

Reliable blood alcohol analysis Optimized primary and confirmation column pair for U.S. blood alcohol analysis Faster GC run times Improved resolution of key ethanol/acetone peaks Available in 0.32 and 0.53 mm id Bonded and cross-linked Test mix available. See pages 9697 for test mix information. Similar Phases: Rtx-BAC1, Rtx-BAC2
GC and GC/MS
DB-ALC1 and DB-ALC2 Chromatograms

Blood Alcohols (Static Headspace/Split) Blood Pollutants page 422 page 423
DB-ALC1 and DB-ALC2

Phase ID (mm) Length (m) Film (m) Temp Limits (C) Part No.
DB-ALC1 DB-ALC1 DB-ALC2 DB-ALC2
0.32 0.53 0.32 0.53
30 30 30 30
1.80 3.00 1.20 2.00
20 to 260/280 20 to 260/280 20 to 260/280 20 to 260/280
123-9134 125-9134 123-9234 125-9234
272
GC Columns
Life Sciences
HP-Blood Alcohol Column

Reliable blood alcohol analysis Excellent confirmation column with DB-ALC2 for method using t-butanol as internal standard
HP-Blood Alcohol Column

0.32
7.5
20
-60 to 270/290
19091S-510
DB-EVDX
Specially configured and tested for drugs of abuse confirmation DB-1-EVDX: 100% Dimethylpolysiloxane DB-5ms-EVDX: Equivalent to (5%-Phenyl)-methylpolysiloxane DB-17-EVDX: (50%-Phenyl)-methylpolysiloxane Consistent retention and peak shape Test mix includes: caffenine, gluthethimide, lidocaine, phenobarbital, EDDP, methaqualone, methadone, cocaine, desiprmine, carbamazepine Low bleed for GC/MS analysis Bonded and cross-linked Solvent rinsable
DB-EVDX Chromatograms
Anesthetics page 417 Sedative Hypnotics page 420
GC and GC/MS
DB-EVDX
DB-1 EVDX DB-5ms EVDX DB-17 EVDX
0.20 0.20 0.20
25 25 25
0.33 0.33 0.33
-60 to 325/350 -60 to 325/350 40 to 280/300
128-8122 128-8522 128-8722
HP-Fast Residual Solvent Column

Equivalent to USP Phase G43 Thinner film reduces run time by 2.5 times and increases MDL by 2 times compared to standard film thickness used for this method Bonded and cross-linked Similar Phases: DB-624, PE-624, 007-624, 007-502, CP-624, ZB-624
HP Fast Residual Solvents Chromatograms

Fast GC Analysis of USP Solvents page 424
HP Fast Residual Solvents

0.53
30
1.00
-20 to 260
19095V-420
273
GC Columns
Pesticides
Pesticides
J&W Scientific low bleed columns are ideal for the analysis of pesticides. Not only do they possess less bleed than a standard polymer which improves the signal to noise ratio and minimum detectable quantities, but they also have higher upper temperature limits which allow for faster run times. See pages 345347 for retention information for 100 common pesticides analyzed on several of our low bleed phases. Agilent also offers several common phases with additional pesticide specific testing to ensure performance for your application. Recommended Columns for Pesticides DB-35ms (P/N 123-3832) and DB-XLB (PN 122-1236) for CLP pesticides, chlorinated herbicides, and EPA Method 508.1 pesticides (pages 349, 351) Also ideal for other dual ECD applications such as 8082 PCBs (Aroclors) and haloacetic acids DB-5ms (PN 122-5532) and DB-35ms (P/N 122-3832) for organophosphorous pesticides (EPA Method 8141A) (pages 356357) HP-5ms for over 550 pesticides using retention time locking software and database See page 317 for Environmental/EPA Method Guide for more column recommendations (pages 317318)
HP-PAS5 and DB-1701P

HP-PAS5: (5%-Phenyl)-methylpolysiloxane Non-polar DB-1701: (14%-Cyanopropyl-phenyl)-methylpolysiloxane Low/midpolarity Exact replacement of HP-PAS1701 Specifically designed and processed for the analysis of organochlorine pesticides ECD tested to assure minimal pesticide breakdown and low ECD bleed Bonded and cross-linked Solvent rinsable Similar Phases: HP-PAS5: DB-5, Ultra-2, SPB-5, CP-Sil 8CB, RSL-200, Rtx-5, BP-5, CB-5, OV-5, 007-2 (MPS-5), SE-52, SE-54, XTI-5, PTE-5, HP-5MS, CC-5, ZB-5 DB-1701P: SPB-1701, CP-Sil 19CB, Rtx-1701, BP-10, CB-1701, OV-1701, 007-1701
GC and GC/MS
274
GC Columns
Pesticides
HP-PAS5
0.32
25
0.52
-60 to 325/350
19091S-010
DB-1701P
0.25 0.32 0.32 0.53
30 25 30 30
0.25 0.25 0.25 1.00
-20 to 280/300 -20 to 280/300 -20 to 280/300 -20 to 260/280
122-7732 123-7722 123-7732 125-7732
DB-608
Specifically designed for the analysis of chlorinated pesticides and PCBs U.S. EPA Methods: 608, 508, 8080 Excellent inertness and recoveries without pesticide breakdown Bonded and cross-linked Solvent rinsable Exact replacement of HP-608 Test mix available. See pages 9697 for test mix information.
GC and GC/MS
Similar Phases: SPB-608, NON-PAKD Pesticide, 007-608
Organochlorine Pesticides page 353
DB-608
0.25 0.32 0.45 0.45 0.53 0.53 0.53
30 30 30 30 15 30 30
0.25 0.50 0.42 0.70 0.83 0.50 0.83
40 to 280/300 40 to 280/300 40 to 260/280 40 to 260/280 40 to 260/280 40 to 260/280 40 to 260/280
122-6832 123-1730 124-6837* 124-1730* 125-1710 125-6837 125-1730
275
GC Columns
Pesticides
J&W Dual Columns

Specific configurations for pesticides, PCBs and volatile priority pollutants No assembly required! Includes two columns, three-way union and 10 meter 0.53 mm id guard column that are connected Matched column dimensions for even sample splitting Leak-free Y connections Performance tested Meets U.S. EPA Method requirements
Dual Columns Chromatograms

Organochlorine Pesticides, DB-5/1701P Dual Column page 355
DB-5/DB-1701P
DB-5 DB-1701P
0.32 0.32
30 30
0.25 0.21
40 to 280/300
009-5007
DB-17/DB-1701P
GC and GC/MS
DB-17 DB-1701P
0.32 0.32
30 30
0.25 0.21
40 to 280/300
009-1707
DB-5/DB-17
DB-5 DB-17
0.32 0.32
30 30
0.25 0.25
40 to 280/300
009-5017
276
GC Columns
Petroleum
Petroleum
Petroleum applications vary greatly in character. From the noble gases to simulated distillation, Agilent offers a broad range of columns designed to meet the needs of the petroleum/petrochemical chromatographer. Refer to the PLOT column section for columns for the analysis of light gases. Recommended Columns for Petroleum DB-HT Sim Dis for Simulated Distallation (page 441) HP-PONA, DB-5 or HP-1 for PONA and PIANO analysis (page 437438) See PLOT columns for recommendations for light hydrocarbons (page 286) See pages 318322 for ASTM Method Guide for more column recommendations
DB-2887
100% Dimethylpolysiloxane Specifically designed for simulated distillation using ASTM Method D2887 Rapid conditioning, fast run time and low bleed when compared to packed columns Bonded and cross-linked Solvent rinsable Test mix available. See pages 9697 for test mix information. Similar Phases: HP-1, Petrocol EX2887, MXT-2887, MXT-1
GC and GC/MS
Reference Gas Oil Simulated Distillation page 439 page 439
DB-2887
I.D. Length Film Temp Limits (C) Part No.
0.45 0.53 0.53
10 10 30
2.55 3.00 3.00
-60 to 350 -60 to 350 -60 to 350
124-2814* 125-2814 125-2834
277
GC Columns
Petroleum
DB-HT Sim Dis

100% dimethylpolysiloxane Boiling point phase for high temperature simulated distillation Durable stainless steel tubing 430C upper temperature limit Distillation range of C6 to C110+ Low bleedeven at 430C! Bonded and cross-linked Solvent rinsable Test mix available. See pages 9697 for test mix information. Similar Phases: Petrocol EX2887, CP-SimDist Ultimetal, MXT-2887, Rtx-2887, AC Controls High Temp Sim Dist, AT-2887
DB-HT Sim Dis Chromatograms

n-Paraffin Standard page 441
DB-HT Sim Dis

0.53
0.15
-60 to 400/430
145-1001
GC and GC/MS
HP-PONA
100% Dimethylpolysiloxane Configured for the analysis of petroleum process products Tested to ensure the resolution of m-xylene from p-xylene and of cyclopentane from 2, 3-dimethylbutane PONA, PIANO High resolution Bonded and cross-linked Solvent rinsable Test mix available. See pages 9697 for test mix information. Similar Phases: Petrocol DH, HP-PONA, SPB-1, 007-1, Rtx-1, MXT-1 Note: 100 psi regulator required to reach optimum carrier gas velocity
278
GC Columns
Petroleum
HP-PONA Chromatograms
Refinery Gas Sulfur Compounds in Naptha page 431 page 434
HP-PONA and Recommended PONA Columns

HP-PONA HP-1 HP-1
0.20 0.20 0.25
50 50 100
0.50 0.50 0.50
-60 to 325/350 -60 to 325/350 -60 to 325/350
19091S-001 19091Z-205 19091Z-530
DB-Petro
100% Dimethylpolysiloxane Configured for the analysis of petroleum process products PONA, PIANO High resolution Bonded and cross-linked Solvent rinsable Test mix available. See pages 9697 for test mix information. Similar Phases: Petrocol DH, HP-PONA, SPB-1, 007-1, Rtx-1, MXT-1
GC and GC/MS
Note: 100 psi regulator required to reach optimum carrier gas velocity
DB-Petro Chromatograms
Propylene, Butene-1 and Ethylene page 428 Regular Unleaded Gasoline page 440 (California Phase 1) Normal GC Run Unleaded Gasoline PONA Mix as Specified by AFNOR Method #2 page 437 page 438
DB-Petro
I.D. Length Film Temp Limits (C) Part No.
0.20 0.25
50 100
0.50 0.50
-60 to 325/350 -60 to 325/350
128-1056 122-10A6
279
GC Columns
Semivolatiles
Semivolatiles
Recommended Columns for Semivolatiles HP-5ms for EPA methods 8270 and 525 (page 366) DB-XLB for PCB congeners (pages 361362) HP-5ms or DB-35ms for PAHs DB-5ms or DB-XLB for phenols (page 369) See pages 317318 for Environmental/EPA Method Guide for more column recommendations
DB-Dioxin
Specifically engineered for the analysis of polychlorinated dibenzodioxins (PCDDs) and dibenzofurans (PCDFs) Resolves 2,3,7,8-TCDD and 2,3,7,8-TCDF from all other isomers in one run Low bleed Bonded and cross-linked Solvent rinsable Test mix available. See pages 9697 for test mix information. Similar Phases: SP-2331, 007-23, Rtx-2332, CP-Sil 88 Note: 100 psi regulator required to reach optimum carrier gas velocity
GC and GC/MS
DB-Dioxin Chromatograms
Dioxins and Furans page 360
DB-Dioxin
0.25 0.25 0.32 0.32
60 60 60 60
0.15 0.25 0.15 0.25
40 to 250/270 40 to 250/270 40 to 250/270 40 to 250/270
122-2461 122-2462 123-2461 123-2462
280
GC Columns
Semivolatiles
DB-5.625
Equivalent to a (5%-Phenyl)-methylpolysiloxane Non-polar Specially processed to exhibit excellent inertness for EPA Semivolatiles Methods 625, 1625, 8270 and CLP protocols* Surpasses EPA performance criteria for semivolatiles Inert for base, neutral and acidic compounds High temperature limit with excellent thermal stability and low bleed Bonded and cross-linked Solvent rinsable
* Pentachlorophenol, 2, 4-Dinitrophenol, Carbazole, and N-Nitrosodiphenylamine used to test response factors.
Test mix available. See pages 9697 for test mix information. Similar Phases: XTI-5, Rtx-5, PTE-5
DB-5.625 Chromatograms
European Red List Volatiles page 377
DB-5.625
0.25 0.25 0.25 0.25 0.32 0.32 0.32 0.53
30 30 30 60 30 30 30 30
0.25 0.50 1.00 0.25 0.25 0.50 1.00 1.00
-60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 300/320
122-5631 122-5632 122-5633 122-5661 123-5631 123-5632 123-5633 125-5632
GC and GC/MS
281
GC Columns
Volatiles
Volatiles
The DB-VRX, an industry standard, handles the extensive target list of volatiles in less than half the time as the standard 105m columnswith the fewest coelutions compared to any other commercially available GC column! Another member of the J&W volatiles family is the DB-MTBE which resolves the highly publicized oxygenated fuel additives. Recommended Columns for Volatiles DB-VRX and DB-624 for EPA methods 502.2 and 8021 (pages 373374) DB-VRX (60 m, 0.25 mm id) for GC/MS volatiles methods (page 371) DB-VRX (20 m, 0.18 mm id) for fast GC/MS volatiles analysis using a 5973 MSD (page 372) (Not recommended for Ion Trap MS or older MSDs) DB-MTBE for extended EPA method 8020 (page 347) DB-TPH for the analysis of BTEX and gasoline total petroleum hydrocarbons See pages 317318 for Environmental/EPA Method Guide for more column recommendations
DB-VRX
Unique selectivity engineered for optimum resolution of volatiles analysis: U.S. EPA Methods 502.2, 524.2 and 8260 0.45 mm id columns provide more plates per meter compared to 0.53 mm id columns for the fewest coelutions for GC method (an industry first)** No subambient cooling required to resolve the six gases Fast run time: < 30 minutes for optimum sample throughput < 8 minutes with 0.18 mm id Low polarity Excellent peak shape Bonded and cross-linked Solvent rinsable
** Two coelutions: 1) m- and p-xylene, for which U.S. EPA does not require separation, and 2) 1,1,2,2-tetrachloroethane and o-xylene which are separated by detectors PID and ELCD, respectively. Note to GC/MS analysts: These coeluting compounds have different primary characteristic ions of 83 and 106, respectively.
GC and GC/MS
Test mix available. See pages 9697 for test mix information. Similar Phases: VOCOL, NON-PAKD, Rtx-Volatiles, PE-Volatiles, 007-624, HP-624, CP-624, Rtx-VRX, Rtx-VGC
DB-VRX Chromatograms
EPA Method 504 by GC/MS EPA Volatiles by GC/MS (Split Injector) EPA Method 8021 High Speed VOC EPA 8260 page 376 page 371 page 373 page 372 Unleaded Gasoline page 348
DB-VRX
0.18 0.18 0.25
20 40 30
1.00 1.00 1.40
-10 to 260 -10 to 260 -10 to 260
121-1524 121-1544 122-1534
282
GC Columns
Volatiles
DB-VRX (continued)
0.25 0.32 0.32 0.45 0.45
60 30 60 30 75
1.40 1.80 1.80 2.55 2.55
- 10 to 260 - 10 to 260 - 10 to 260 - 10 to 260 - 10 to 260
122-1564 123-1534 123-1564 124-1534* 124-1574*
DB-624
Specifically designed for the analysis of volatile priority pollutants No cryogenics needed for U.S. EPA Method 502.2 Excellent for U.S. EPA Methods: 501.3, 502.2, 503.1, 524.2, 601, 602, 8010, 8015, 8020, 8240, 8260 Excellent inertness for active compounds Bonded and cross-linked Solvent rinsable Exact replacement of HP-624 Equivalent to USP Phase G43 Test mix available. See pages 9697 for test mix information. Similar Phases: AT-624, Rtx-624, PE-624, 007-624, 007-502, CP-624, ZB-624
GC and GC/MS
1,3-Butadiene Alcohols EPA Method 504 by GC/MS EPA Volatiles GC/MS EPA Method 8021 Esters page 428 page 394 page 376 page 371 page 373 page 402 Ethers Fast VOA Analysis Glycols Halogenated Hydrocarbon Nitrogen Based Solvents Residual Solvents, USP <467> page 403 page 375 page 403 page 405 page 409 page 424
DB-624
0.18 0.20 0.25 0.25 0.32 0.32 0.45 0.45 0.53 0.53 0.53
20 25 30 60 30 60 30 75 30 60 75
1.00 1.12 1.40 1.40 1.80 1.80 2.55 2.55 3.00 3.00 3.00
-20 to 260 -20 to 260 -20 to 260 -20 to 260 -20 to 260 -20 to 260 -20 to 260 -20 to 260 -20 to 260 -20 to 260 -20 to 260
121-1324 128-1324 122-1334 122-1364 123-1334 123-1364 124-1334* 124-1374* 125-1334 125-1364 125-1374
283
GC Columns
Volatiles
HP-VOC
Selectivity engineered for: U.S. EPA Methods 502.2, 524.2 and 8260 Low polarity: slightly more polar than DB-VRX Excellent peak shape Bonded and cross-linked Solvent rinsable Similar Phases: DB-VRX, NON-PAKD, Rtx-Volatiles, PE-Volatiles, 007-624, HP-624, CP-624, Rtx-VRX, Rtx-VGC
HP-VOC
0.20 0.20 0.32 0.32 0.32 0.53 0.53
30 60 60 90 90 90 105
1.12 1.12 1.80 1.00 1.80 3.00 3.00
-60 to 280/290 -60 to 280/290 -60 to 280/290 -60 to 280/290 -60 to 280/290 -60 to 280/290 -60 to 280/290
19091R-303 19091R-306 19091R-316 19091R-219 19091R-319 19095R-429 19095R-420
GC and GC/MS
DB-502.2
Available in 105 meters for volatiles analyses Excellent peak shape Bonded and cross-linked Solvent rinsable Test mix available. See pages 9697 for test mix information. Similar Phases: Rtx-502.2, VOCOL, HP-VOC
DB-502.2
0.25 0.32 0.45 0.45 0.53
60 60 75 105 105
1.40 1.80 2.55 2.55 3.00
0 to 260/280 0 to 260/280 0 to 260/280 0 to 260/280 0 to 260/280
122-1464 123-1464 124-1474* 124-14A4* 125-14A4
284
GC Columns
Volatiles
DB-MTBE
Low polarity stationary phase Resolves MTBE from 2-methylpentane and 3-methylpentane for better quantitation Engineered for purge and trap injection without the need for cryofocusing Bonded and cross-linked Solvent rinsable Test mix available. See pages 9697 for test mix information. Similar Phases: None
DB-MTBE Chromatograms
Methyl-tert-butyl Ether (MTBE) page 347 FID, Extended 8020 Analysis Methyl-tert-butyl Ether (MTBE) page 347 PID, Extended 8020 Analysis
DB-MTBE
0.45 0.53
30 30
2.55 3.00
35 to 260/280 35 to 260/280
124-0034* 125-0034
DB-TPH
Specifically designed for the analysis of total petroleum hydrocarbons (TPHs), soil analysis, and LUFT Three analyses in one injection: gas range organics, diesel range organics and motor oil Fast run time Bonded and cross-linked Solvent rinsable Test mix available. See pages 9697 for test mix information. Similar Phase: None
GC and GC/MS
DB-TPH
0.32 0.45
30 30
0.25 1.00
-10 to 320 -10 to 290
123-1632 124-1632*
285
GC Columns
PLOT Columns
PLOT Columns
PLOT columns are ideal for separating compounds that are gases at room temperatures. Agilent Technologies offers a comprehensive line of PLOT columns for analysis of fixed gases, low molecular weight hydrocarbon isomers, volatile polar compounds and reactive analytes such as sulfur gases, amines and hydrides. Our PLOT phases are offered in dimensions from 0.25 to 0.53 mm id, allowing for easy column selection for various detector and system requirements. For GC/MS systems, we offer several small diameter columns with truly bonded and immobilized stationary phases, eliminating potential detector fouling due to particle generation.
PLOT Column Application Recommendations

Column Stationary Phase Typical Applications
HP-PLOT Molesieve
5 molecular sieve zeolite
Permanent and noble gases. Thick and thin films available. Thick film column will resolve argon and oxygen at 35C. Least polar Al2O3 phase. Lowest retention of olefins relative to comparable paraffin. C1 to C8 hydrocarbon isomers. Column of choice for accurate quantitation of dienes, especially propadiene and butadiene from ethylene and propylene streams. Excellent general use Al2O3 column. C1 to C8 hydrocarbon isomers. Best for resolving acetylene from butane and propylene from isobutane. Most polar of the Al2O3 columns. Highest retention of olefins relative to comparable paraffin. Excellent general use Al2O3 column. C1 to C8 hydrocarbon isomers. Best for resolving cyclopropane from propylene. Good stability and recovery from water saturation. C1 to C3 isomers, alkanes to C12, CO2, methane, air/CO, water, oxygenated compounds, sulfur compounds, solvents. More polar than HP-PLOT Q and GS-Q. C1 to C7 hydrocarbons, CO2, methane, air/CO, water, glycol dimethacrylate oxygenates, amines, solvents, alcohols, ketones, aldehydes. C1 to C12 hydrocarbons, CO2, trace-level sulfurs, hydride gases, inorganic gases, halocarbons, SF6, oxygen/nitrogen separation at 80C. C1 to C5 hydrocarbons, CO2, air/CO, trace acetylene in ethylene, methane.
HP-PLOT Al2O3 KCl Aluminum oxide deactivated with KCl
GC and GC/MS
HP-PLOT Al2O3 S
Aluminum oxide deactivated with sodium sulfate
GS-Alumina
Aluminum oxide with proprietary deactivation
HP-PLOT Q
Polystyrene-divinylbenzene
HP-PLOT U
Divinylbenzene/ethylene
GS-GasPro
Proprietary, bonded silica-based
GS-CarbonPLOT
Bonded, monolithic carbon-layer
286
GC Columns
PLOT Columns
HP-PLOT Al2O3 KCl

Least polar Alumina phase Aluminum oxide deactivated with KCl Standard column choice for light hydrocarbon analysis: C1 to C8 hydrocarbon isomers Low retention of olefins relative to comparable paraffin Excellent for quantitation of dienes, especially propadiene and butadiene from ethylene and propylene streams Recommended phase for many ASTM methods Prefered KCl deactivated Alumina Similar Phases: CP-Al2O3/KCl PLOT, Rt-Alumina PLOT, Alumina PLOT, Al2O3/KCl
HP-PLOT Al2O3 KCl

0.25 0.32 0.53 0.53
30 50 30 50
5.00 8.00 15.00 15.00
-60 to 200 -60 to 200 -60 to 200 -60 to 200
19091P-K33 19091P-K15 19095P-K23 19095P-K25
GS-Alumina KCl
Least polar Alumina phase Aluminum oxide deactivated with KCl Good choice for light hydrocarbon analysis Good resolution of propadiene and butadiene from ethylene and propylene streams Similar Phases: CP-Al2O3/KCl PLOT, Rt-Alumina PLOT, Alumina PLOT, Al2O3/KCl
GC and GC/MS
GS-Alumina KCl Chromatograms

Impurities in Ethylene Impurities in Propylene page 426 page 427
GS-Alumina KCl
ID (mm) Length (m) Temp Limits (C) Part No.
0.53 0.53
30 50
-60 to 200 -60 to 200
115-3332 115-3352
287
GC Columns
PLOT Columns
HP-PLOT Al2O3 S
Middle range of polarity for Alumina phases Aluminum oxide deactivated with sodium sulfate Excellent general use column for light hydrocarbon analysis: C1 to C8 hydrocarbon isomers Best for resolving acetylene from butane and propylene from isobutane Similar Phases: CP-Al2O3 PLOT Na2SO4
HP-PLOT Al2O3 S Chromatograms

Ethylene Natural Gas page 426 page 425 Refinery Gas page 431
HP-PLOT Al2O3 S
0.25 0.25 0.32 0.53 0.53 0.53
15 30 50 15 30 50
5.00 5.00 8.00 15.00 15.00 15.00
-60 to 200 -60 to 200 -60 to 200 -60 to 200 -60 to 200 -60 to 200
19091P-S31 19091P-S33 19091P-S15 19095P-S21 19095P-S23 19095P-S25
GC and GC/MS
GS-Alumina
Most polar Alumina phase Aluminum oxide with proprietary deactivation Excellent general use column for light hydrocarbon analysis: C1 to C8 hydrocarbon isomers Separates C1-C4 saturated and unsaturated hydrocarbons Best for resolving cyclopropane from propylene Faster, more efficient and provides more sensitivity than packed equivalents Minimal conditioning time required* Preferred substitution for sodium sulfate deactivated Alumina because of its regenerative nature Note: Alumina columns have a tendency to adsorb water and CO2 which, over time, results in changes in retention time. We use an advanced, proprietary deactivation process which allows for rapid regeneration. Fully water saturated GS-Alumina columns regenerate in 7 hours or less at 200C. Similar Phases: Al2O3/KCl, Al2O3/Na2SO4, Rt-Alumina PLOT, Alumina PLOT
GS-Alumina Chromatograms
1,3-Butadiene Purity page 429 Extended Hydrocarbon Analysis page 430 Propylene page 427
288
GC Columns
PLOT Columns
GS-Alumina
0.53 0.53
30 50
-60 to 200 -60 to 200
115-3532 115-3552
HP-PLOT Al2O3 M
Most polar Alumina phase (similar to GS-Alumina) Aluminum oxide deactivated with proprietary deactivation Good general use column for light hydrocarbon analysis: C1 to C8 hydrocarbon isomers Good for resolving acetylene from butane and propylene from isobutane Similar Phases: GS-Alumina
HP-PLOT Al2O3 M
0.32 0.53 0.53
50 30 50
8.00 15.00 15.00
-60 to 200 -60 to 200 -60 to 200
19091P-M15 19095P-M23 19095P-M25
GS-GasPro
GC and GC/MS
Unique bonded PLOT column technology Excellent choice for light hydrocarbons and sulfur gases Retention stability not affected by water Separates CO and CO2 on a single column Ideal PLOT column for GC/MSno particles Similar Phase: CP-Silica PLOT
GS-GasPro Chromatograms
C1 and C2 Halocarbons (Freons) page 380 Extended Hydrocarbon page 430 Analysis GS-Alumina and GasPro Halocarbons page 413 Haloethane page 414 Inorganic Gases page 414 Mercaptans Sulfur Compounds in Propylene (1 ppm) Sulfur Gas Analysis in Light Hydrocarbon Streams page 434 page 434 page 433
GS-GasPro
0.32 0.32 0.32 0.32
5 15 30 60
-80 to 260/300 -80 to 260/300 -80 to 260/300 -80 to 260/300
113-4302 113-4312 113-4332 113-4362
289
GC Columns
PLOT Columns
GS-CarbonPLOT
High stability, bonded carbon layer stationary phase Unique selectivity for inorganic and organic gases Extended temperature limit of 360C Similar Phases: Carbopack, CLOT, Carboxen-1006 PLOT, CP-CarboPLOT P7
GS-CarbonPLOT
0.32 0.32 0.32 0.32 0.53 0.53
15 30 30 60 15 30
1.50 1.50 3.00 1.50 3.00 3.00
0 to 360 0 to 360 0 to 360 0 to 360 0 to 360 0 to 360
113-3112 113-3132 113-3133 113-3162 115-3113 115-3133
HP-PLOT Molesieve
GC and GC/MS
A PLOT column for the analysis of permanent gases O2, N2, CO and CH4 resolve in less than 5 minutes Durable molecular sieve 5 coating minimizes baseline spiking and damage to multiport valves Select a thick film for Ar/O2 separation without cryogenic cooling Select thin film HP-PLOT Molesieve columns for routine air monitoring applications Replaces GS-Molesieve Note: Molecular sieve columns will absorb water which, over time, results in changes in retention time. We use an advanced, proprietary deactivation process which allows for rapid regeneration. Fully saturated HP-PLOT Molesieve columns regenerate in 7 hours or less at 200C.
HP-PLOT Molesieve Chromatograms

Noble Gases page 425 Permanent Gases page 425
HP-PLOT MoleSieve
0.32 0.32 0.32 0.32 0.53 0.53 0.53 0.53
15 30 15 30 30 15 30 15
12.00 12.00 25.00 25.00 50.00 25.00 25.00 50.00
-60 to 300 -60 to 300 -60 to 300 -60 to 300 -60 to 300 -60 to 300 -60 to 300 -60 to 300
19091P-MS3 19091P-MS4 19091P-MS7 19091P-MS8 19095P-MS0 19095P-MS5 19095P-MS6 19095P-MS9
290
GC Columns
PLOT Columns
HP-PLOT Q
Bonded polystyrene-divinylbenzene based column A PLOT column with polarity between Porapak-Q and Porapak-N Excellent column for C1 to C3 isomers and Alkanes to C12, CO2, methane, air/CO, oxygenated compounds, sulfur compunds and solvents A PLOT column to replace packed gas-solid columns Separates ethane, ethylene and ethyne (acetylene) Improved resolution in less time than conventional packed columns Minimal conditioning time required1 hour Preferred Q column due to its robust nature Similar Phases: CP PoraPLOT Q, CP PoraPLOT Q-HT, Rt-QPLOT, SupelQ PLOT, GS-Q
HP-PLOT Q Chromatograms
Baseline Resolution of Air/CO, page 425 CO2, and Methane in Natural Gas Sample Ethylene Oxide page 436 Synthetic Standard Oxygenates Separation of Refinery Gas Sample page 436 page 431
HP-PLOT Q
0.32 0.32 0.53 0.53
15 30 15 30
20.00 20.00 40.00 40.00
-60 to 270/290 -60 to 270/290 -60 to 270/290 -60 to 270/290
19091P-QO3 19091P-QO4 19095P-QO3 19095P-QO4
GC and GC/MS
Particle Traps for Use with PLOT Columns

Though highly stabilized, it is impossible to guarantee that no particles will dislodge from the column wall. When used in valve-switching applications, the use of a particle trap can prevent scarring of the rotors.
Particle Traps
ID (mm) Length (m) Part No.
0.32 0.53
2.5 2.5
5181-3351 5181-3352
291
GC Columns
PLOT Columns
GS-Q
Porous divinylbenzene homopolymer A PLOT column with polarity between Porapak-Q and Porapak-N Separates ethane, ethylene and ethyne (acetylene) Not recommended for quantification of polar compounds Minimal conditioning time required1 hour Similar Phases: CP PoraPLOT Q, CP PoraPLOT Q-HT, Rt-QPLOT, SupelQ PLOT, HP-Q PLOT Note: GS-Q has been proven useful for separations of various gases including freons and sulfides (except for SO2). Although GS-Q is not suitable for quantitating water or alcohols, aqueous injections will not harm the column.
GS-Q Chromatograms
Sulfur Gas Analysis in Light Hydrocarbon Streams page 433
GS-Q
0.32 0.32 0.32 0.53 0.53 0.53 0.53
15 25 30 10 15 25 30
-60 to 250 -60 to 250 -60 to 250 -60 to 250 -60 to 250 -60 to 250 -60 to 250
113-3412 113-3422 113-3432 115-34H2 115-3412 115-3422 115-3432
GC and GC/MS
HP-PLOT U
Bonded divinylbenzene/ethylene glycol dimethacrylate More polar than HP-PLOT Q Excellent column for C1 to C7 hydrocarbons, CO2, methane, air/CO, water, oxygenates, amines, solvents, alcohols, ketones, and aldehydes Improved resolution in less time than conventional packed columns Similar Phases: PoraPlot U, RTU PLOT
HP-PLOT U
0.32 0.32 0.53 0.53
15 30 15 30
10 10 20 20
-60 to 190 -60 to 190 -60 to 190 -60 to 190
19091P-UO3 19091P-UO4 19095P-UO3 19095P-UO4
292
GC Columns
Metal Columns
Metal Columns DB-ProSteel Series Stainless Steel GC Columns

Excellent inertness Virtually unbreakable Available in a wide variety of stationary phases Bonded and cross-linked Ideal for high temperature analysis and process applications Our easy to handle DB-ProSteel metal columns are deactivated with a new formula (this is not glass lined steel) to provide inertness which truly rivals fused silica. DB-ProSteel metal columns can be custom wound upon request for small GC ovens. Several of our most popular bonded phases are available in metal. DB-ProSteel GC columns have the same outer diameter as standard Megabore (0.53 mm id), so no special ferrules are required.
DB-ProSteel
DB-PS1 DB-PS1 DB-PS1 DB-PS1 DB-PS1 DB-PS1 DB-PS1 DB-PS1 DB-PS5 DB-PS624 DB-PS2887 DB-PSWAX DB-PSWAX
0.53 0.53 0.53 0.53 0.53 0.53 0.53 0.53 0.53 0.53 0.53 0.53 0.53
15 15 15 30 30 30 30 30 30 30 10 15 30
0.15 1.50 0.50 0.15 1.50 3.00 5.00 0.50 1.50 3.00 3.00 1.00 1.00
-60 to 340/360 -60 to 300/320 -60 to 300/320 -60 to 340/360 -60 to 300/320 -60 to 260/280 -60 to 260/280 -60 to 300/320 -60 to 300/320 -20 to 260 -60 to 350 20 to 230/240 20 to 230/240
145-1011 145-1012 145-1017 145-1031 145-1032 145-1034 145-1035 145-1037 145-5032 145-1334 145-2814 145-7012 145-7032
GC and GC/MS
293
GC Columns
Non-Bonded Phases
Non-Bonded Phases
Whenever possible Agilent recommends the use of bonded and cross-linked columns. Bonded columns are more rugged, will have longer lifetimes and can be solvent rinsed. However, Agilent recognizes that some methods have been developed on these non-bonded phases and therefore maintains these columns to support established methods.
HP-101
100% polydimethylsiloxane Similar Phases: OV-101, SP 200, DB-1, HP-1 Because HP-101 columns are not bonded or cross-linked, we do not recommend solvent rinsing.
HP-101
0.20 0.20 0.20 0.32 0.32
12 25 50 25 50
0.25 0.20 0.20 0.30 0.30
0 to 280 0 to 280 0 to 280 0 to 280 0 to 280
19091-60010 19091Y-102 19091Y-105 19091Y-012 19091Y-015
GC and GC/MS
HP-1 is the recommended bonded replacement for HP-101, see page 235
HP-17
50% Phenyl and 50% Methyl siloxane Similar Phases: OV-17, SP-2250, DB-17, HP-50+ Because the HP-17 is not bonded or cross-linked, we do not recommend solvent rinsing.
HP-17
0.53
10
2.00
25 to 260/280
19095L-121
294
GC Columns
Non-Bonded Phases
Carbowax 20M and HP-20M

Polyethylene glycol, MW 20,000 Equivalent to USP Phase G16 Similar Phases: DB-WAX, HP-INNOWax, DB-WAXetr Because the Carbowax 20M and HP-20M are not bonded or cross-linked, we do not recommend solvent rinsing.
Carbowax 20M
0.25 0.25 0.32 0.32
30 60 30 60
0.25 0.25 0.25 0.25
60 to 220/240 60 to 220/240 60 to 220/240 60 to 220/240
112-2032 112-2062 113-2032 113-2062
DB-WAX is the recommended bonded replacement for HP-20M, see page 260
HP-20M
0.20 0.20 0.32 0.32 0.53 0.53
25 50 25 50 10 30
0.10 0.10 0.30 0.30 1.33 1.33
60 to 220 60 to 220 60 to 220 60 to 220 60 to 220 60 to 220
19091W-102 19091W-105 19091W-012 19091W-015 19095W-121 19095W-123
GC and GC/MS
DB-WAX is the recommended bonded replacement for HP-20M, see page 260
OV-351
Nitroterephthalic acid modified polyethylene glycol Equivalent to USP Phase G35 Similar Phases: DB-FFAP and HP-FFAP Because the OV-351 is not bonded or cross-linked, we do not recommend solvent rinsing.
OV-351
I.D. 0.25 0.32 Length 30 30 Film 0.25 0.25 Temp Limits (C) 60 to 220/240 60 to 220/240 Part No. 112-5132 113-5132
DB-FFAP is the recommended bonded replacement for OV-351, see page 265
295
GC Columns
Non-Bonded Phases
CAM
Base deactivated polyethylene glycol Specifically designed for amine analysis Excellent peak shape for primary amines Replaces HP-Basicwax Test mix available. See pages 9697 for test mix information. Similar Phases: CAM: Stabilwax-DB, Carbowax Amine Because the CAM is not bonded or cross-linked, we do not recommend solvent rinsing.
CAM Chromatograms
Amines in Water Primary Amines page 397 page 396
CAM
0.25 0.25 0.25 0.25 0.32 0.32 0.32 0.53 0.53 0.53
15 30 30 60 15 30 30 15 30 60
0.25 0.25 0.50 0.25 0.25 0.25 0.50 1.00 1.00 1.00
112-2112 112-2132 112-2133 112-2162 113-2112 113-2132 113-2133 115-2112 115-2132 115-2162
GC and GC/MS
296
GC Columns
Non-Bonded Phases
DX-1, DX-3 and DX-4

DX-1: 90% Dimethylpolysiloxane 10% Polyethylene Glycol DX-3: 50% Dimethylpolysiloxane 50% Polyethylene Glycol DX-4: 15% Dimethylpolysiloxane 85% Polyethylene Glycol Test mix available. See pages 9697 for test mix information. Because DX series GC columns are not bonded and cross-linked, we do not recommend solvent rinsing.
DX-1
0.25 0.25 0.32
15 30 30
0.25 1.00 1.00
50 to 250/270 50 to 250/270 50 to 250/270
122-6112 122-6133 123-6133
DX-3
0.25 0.25 0.32
15 30 30
0.25 0.25 0.25
50 to 250/270 50 to 250/270 50 to 250/270
122-6312 122-6332 123-6332
GC and GC/MS
DX-4
0.25 0.25 0.25 0.32 0.32 0.32
15 30 60 15 30 60
0.25 0.25 0.25 0.25 0.25 0.25
50 to 250/270 50 to 250/270 50 to 250/270 50 to 250/270 50 to 250/270 50 to 250/270
122-6412 122-6432 122-6462 123-6412 123-6432 123-6462
297
GC and GC/MS
SE Series GC Columns
SE Series GC Columns
SE-30: 100% Dimethylpolysiloxane SE-54: (5%-Phenyl)(1%-Vinyl)-methylpolysiloxane Test mix available. See pages 9697 for test mix information. Because SE series GC columns are not bonded or cross-linked, we do not recommend solvent rinsing.
SE-30
0.25 0.32 0.32
30 30 60
0.25 0.25 0.25
0 to 325/350 0 to 325/350 0 to 325/350
112-3032 113-3032 113-3062
DB-1 is a bonded replacement for SE-30, see page 231
SE-54
0.25 0.25 0.32 0.32
30 60 15 30
0.25 0.25 0.25 0.25
0 to 325/350 0 to 325/350 0 to 325/350 0 to 325/350
112-5432 112-5462 113-5412 113-5432
GC and GC/MS
DB-5 is a bonded replacement for SE-54, see page 238
298
GC Columns
Custom Columns
Custom Columns
Even though we offer over a thousand readily available columns, Agilent recognizes that sometimes you need something a little out of the ordinary. Thats why we developed our Custom Column Shop. So if you cant find what you are looking for in our standard order guides we will design, build and test capillary GC columns for customers to meet your needs. We can create columns with non-standard lengths or unusual film thickness. Well connect columns together in series or as dual columns. We also recognize that sometimes customers have specific column performance requirements for their applications that might not be met with standard test mixes. As a result we can also custom-test your columns with your desired test mixture and test conditions to meet your specific performance requirements. DuraGuard columns with an integrated guard column. Most phases can be manufactured with a built-in guard column which means you get the advantages of a guard column without the union. Custom columns are ordered under the part number 100-2000. Be sure to provide the details of your desired custom service or column including phase, length, id, and film thickness. Contact your local Agilent office or your authorized Agilent Distributor to receive a quote for your Custom Column needs.
GC and GC/MS
299
GC Columns
GC Columns on 5 in. Cage
Columns for the 6850 GC

GC capillary columns are typically wound on 7 in. cages that fit into standard-sized GCs like the 6890. The 6850 GC has a smaller oven which requires the use of columns wound on a smaller cage diameter. The 5 in. cage configurations listed here are available as standard products. If you require a column for your 6850 that is not listed below the column, simply order part number 100-2000 and specify: the standard cage part number, on 5 in. cage. or the phase and dimensions (length, internal diameter, film thickness), on 5 in. cage. The price of the 5 in. cage column will be the same as the same column on a standard cage.
CAM - 5 in. cage

0.25 0.32
30 30
0.50 0.25
60 to 220/240 60 to 220/240
112-2133E 113-2132E
DB-1 - 5 in. cage
GC and GC/MS
ID (mm)
Length (m)
Film (m)
Temp Limits (C)
Part No.
0.10 0.10 0.10 0.25 0.25 0.32 0.32 0.32 0.53 0.53 0.53 0.53
10 10 40 30 30 30 60 60 15 15 30 30
0.10 0.40 0.40 0.25 1.00 1.00 3.00 5.00 0.15 1.50 3.00 5.00
127-1012E 127-1013E 127-1043E 122-1032E 122-1033E 123-1033E 123-1064E 123-1065E 125-1011E 125-1012E 125-1034E 125-1035E
DB-1ms - 5 in. cage

0.10
10
0.40
-60 to 340/360
127-0113E
300
GC Columns
DB-5 - 5 in. cage
0.25 0.32 0.32 0.32 0.53 0.53
30 15 30 60 30 60
0.25 0.25 0.25 1.00 1.50 1.50
-60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 300/320 -60 to 300/320
122-5032E 123-5012E 123-5032E 123-5063E 125-5032E 125-5062E
DB-5ms - 5 in. cage

0.25 0.32
30 30
1.00 0.25
-60 to 325/350 -60 to 325/350
122-5533E 123-5532E
DB-17 - 5 in. cage

0.32
30
0.25
40 to 280/300
123-1732E
DB-225ms - 5 in. cage
GC and GC/MS
ID (mm)
Length (m)
Film (m)
Temp Limits (C)
Part No.
0.25
15
0.25
40 to 220/240
122-2212E
DB-624 - 5 in. cage

0.25 0.25 0.32 0.32 0.53 0.53
30 60 30 60 30 75
1.40 1.40 1.80 1.80 3.00 3.00
-20 to 260 -20 to 260 -20 to 260 -20 to 260 -20 to 260 -20 to 260
122-1334E 122-1364E 123-1334E 123-1364E 125-1334E 125-1374E
DB-1301 - 5 in. cage

0.25 0.32
60 30
1.00 1.00
-20 to 280/300 -20 to 280/300
122-1363E 123-1333E
DB-1701 - 5 in. cage

0.25 0.32 0.32 0.32 0.53
30 30 30 60 30
0.25 0.25 1.00 1.00 1.00
-20 to 280/300 -20 to 280/300 -20 to 280/300 -20 to 280/300 -20 to 260/280
122-0732E 123-0732E 123-0733E 123-0763E 125-0732E
301
GC Columns
DB-1701P - 5 in. cage
0.32
30
0.25
-20 to 280/300
123-7732E
DB-FFAP - 5 in. cage

0.32
30
0.25
40 to 250
123-3232E
DB-MTBE - 5 in. cage

0.53
30
3.00
35 to 260/280
125-0034E
DB-Petro - 5 in. cage

0.25
100
0.50
-60 to 325/350
122-10A6E
DB-PS1 - 5 in. cage

0.53
30
3.00
-60 to 260/280
145-1034E
GC and GC/MS
DB-VRX - 5 in. cage

0.25
60
1.40
-10 to 260
122-1564E
DB-WAX - 5 in. cage

0.10 0.10 0.25 0.25 0.32 0.32 0.32 0.53
10 10 30 30 30 30 60 30
0.10 0.20 0.25 0.50 0.25 0.50 0.50 1.00
20 to 250/260 20 to 240/250 20 to 250/260 20 to 240/250 20 to 250/260 20 to 240/250 20 to 240/250 20 to 230/240
127-7012E 127-7013E 122-7032E 122-7033E 123-7032E 123-7033E 123-7063E 125-7032E
GS-Q - 5 in. cage

0.53
30
-60 to 250
115-3432E
302
GC Columns
HP-1 - 5 in. cage
0.20 0.25 0.25 0.25 0.25 0.25 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.53 0.53 0.53 0.53 0.53 0.53 0.53 0.53 0.53 0.53
25 30 30 60 60 100 15 15 25 25 30 30 30 30 50 60 5 5 10 15 15 15 30 30 30 30
0.33 0.25 1.00 0.25 1.00 0.50 0.25 1.00 0.17 0.52 0.25 1.00 3.00 5.00 0.52 1.00 0.15 2.65 2.65 0.15 3.00 5.00 1.50 2.65 3.00 5.00
-60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 260/280 -60 to 260/280 -60 to 325/350 -60 to 325/350 -60 to 320/360 -60 to 260/280 -60 to 260/280 -60 to 320/360 -60 to 260/280 -60 to 260/280 -60 to 300/320 -60 to 260/280 -60 to 260/280 -60 to 260/280
19091Z-102E 19091Z-433E 19091Z-233E 19091Z-436E 19091Z-236E 19091Z-530E 19091Z-411E 19091Z-211E 19091Z-012E 19091Z-112E 19091Z-413E 19091Z-213E 19091Z-513E 19091Z-713E 19091Z-115E 19091Z-216E
GC and GC/MS
19095Z-220E 19095S-100E 19095Z-121E 19095Z-221E 19095Z-421E 19095Z-621E 19095Z-323E 19095Z-123E 19095Z-423E 19095Z-623E
HP-1ms - 5 in. cage

0.25 0.25 0.25
30 30 60
0.10 0.25 0.25
-60 to 325/350 -60 to 325/350 -60 to 325/350
19091S-833E 19091S-933E 19091S-936E
303
GC Columns
HP-5 - 5 in. cage
0.20 0.25 0.25 0.25 0.32 0.32 0.32 0.32 0.53 0.53 0.53 0.53
25 30 60 60 15 25 30 50 10 30 30 30
0.33 0.25 0.25 1.00 0.25 0.52 0.25 0.52 2.65 0.88 1.50 2.65
19091J-102E 19091J-433E 19091J-436E 19091J-236E 19091J-411E 19091J-112E 19091J-413E 19091J-115E 19095J-121E 19095J-023E 19095J-323E 19095J-123E
HP-5ms - 5 in. cage

0.20
25 30 30 30
0.33 0.10 0.25 1.00
-60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350
19091S-102E 19091S-333E 19091S-433E 19091S-233E
GC and GC/MS
0.25 0.25 0.25
HP-50+ - 5 in. cage

0.32 0.32 0.53
30 30 30
0.25 0.50 1.00
40 to 280/300 40 to 280/300 40 to 260/280
19091L-413E 19091L-113E 19095L-023E
HP-101 - 5 in. cage

0.20 0.20
12 25
0.25 0.20
-60 to 280 -60 to 280
19091-60010E 19091Y-102E
HP Blood Alcohol Column - 5 in. cage

0.32
7.5
20.00
-60 to 270/290
19091S-510E
HP Fast GC Residual Solvents - 5 in. cage

0.53
30
1.00
-20 to 260
19095V-420E
304
GC Columns
HP-FFAP - 5 in. cage
0.20 0.25 0.32 0.32 0.53 0.53
50 30 25 50 15 30
0.30 0.25 0.50 0.50 1.00 1.00
60 to 240/250 60 to 240/250 60 to 240/250 60 to 240/250 60 to 240 60 to 240
19091F-105E 19091F-433E 19091F-112E 19091F-115E 19095F-120E 19095F-123E
HP-INNOWax - 5 in. cage

0.25 0.25 0.25 0.32 0.32 0.32 0.32 0.32 0.53 0.53
30 30 60 30 30 30 60 60 15 30
0.25 0.50 0.25 0.15 0.25 0.50 0.25 0.50 1.00 1.00
19091N-133E 19091N-233E 19091N-136E 19091N-013E 19091N-113E 19091N-213E 19091N-116E 19091N-216E 19095N-121E 19095N-123E
GC and GC/MS
HP-PLOT Al2O3 KCl - 5 in. cage

0.32
50
8.00
-60 to 200
19091P-K15E
HP-PLOT Al2O3 M - 5 in. cage

0.53
50
15.00
-60 to 200
19095P-M25E
HP-PLOT Al2O3 S - 5 in. cage

0.32 0.53
50 50
8.00 15.00
-60 to 200 -60 to 200
19091P-S15E 19095P-S25E
HP-PLOT Molesieve - 5 in. cage

0.32 0.53 0.53
30 30 30
12.00 25.00 50.00
-60 to 300 -60 to 300 -60 to 300
19091P-MS4E 19095P-MS6E 19095P-MS0E
305
GC Columns
HP-PLOT Q - 5 in. cage
0.32 0.53 0.53
25 15 25
20 40 40
-60 to 270/290 -60 to 270/290 -60 to 270/290
19091P-QO4E 19095P-QO3E 19095P-QO4E
HP-PLOT U - 5 in. cage

0.32 0.53
30 30
10 20
-60 to 190 -60 to 190
19091P-UO4E 19095P-UO4E
HP-PONA - 5 in. cage

0.20
50
0.50
-60 to 325/350
19091S-001E
Ultra 1 - 5 in. cage

0.20
25
0.33
-60 to 325/350
19091A-102E
GC and GC/MS
Ultra 2 - 5 in. cage

0.20 0.32
25 25
0.33 0.17
-60 to 325/350 -60 to 325/350
19091B-102E 19091B-012E
Cage for 68550

Description 5 in. cage for 6850, 13 cm/5 in. od Part No. 19091-80060
306
GC Columns
Quality Control Specifications
The Tightest QC Specifications in the Industry

Highest level of column-to-column reproducibility Decreased column bleed for increased sensitivity All J&W Scientific columns are individually tested and must live up to our stringent QC requirements We take column manufacturing seriously and strive to make the best columns possible. And to prove it, QC for column bleed, retention index window, and plates per meter are published for our most popular phases and column configurations. The individual bleed specifications represent the difference, in pico amperes of the FID response, measured at the upper temperature limit of the column and the isothermal test temperature (generally 110C to 125C). We strive for the lowest bleed GC columns in the industry. The specifications for RI represent our narrow retention index windows. A small number means you get the highest level of column-to-column reproducibility. Plates per meter is a measurement of the resolving potential for a GC column. We publish the minimum plate specifications that a column must have before it can become a J&W capillary column.

ID (mm) DB-1 0.18 0.18 0.18 0.18 0.18 0.20 0.20 0.20 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 10 10 20 20 40 12 25 50 15 15 15 30 30 30 60 60 60 15 15 15 25 30 30 30 60 60 60 0.18 0.40 0.18 0.40 0.40 0.33 0.33 0.33 0.10 0.25 1.00 0.10 0.25 1.00 0.10 0.25 1.00 0.10 0.25 1.00 0.52 0.10 0.25 1.00 0.10 0.25 1.00 4 6 6 6 8 4 6 8 4 4 10 6 6 15 7 7 15 4 4 10 15 6 6 15 8 8 15 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 5,150 4,850 5,150 4,850 4,600 4,800 4,800 4,650 3,900 3,900 3,500 3,900 3,900 3,500 3,700 3,700 3,300 3,100 3,100 3,000 3,100 3,100 3,100 3,000 3,000 3,000 2,800 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 121-1012 121-1013 121-1022 121-1023 121-1043 128-1012 128-1022 128-1052 122-1011 122-1012 122-1013 122-1031 122-1032 122-1033 122-1061 122-1062 122-1063 123-1011 123-1012 123-1013 123-1026 123-1031 123-1032 123-1033 123-1061 123-1062 123-1063 Length (m) Film Thickness (m) Maximum Allowable Bleed (pA) RI Tolerance (+/- I) Minimum Plates/ Meter Temperature Limits (C)
GC and GC/MS
Part No.
307
GC Columns
Quality Control Specifications (continued)
ID (mm) DB-1ms 0.20 0.20 0.25 0.25 0.25 0.25 0.32 0.32 0.32 0.32 DB-5 0.18 0.18 0.18 0.18 0.18 0.20 0.20 0.20 0.25 0.25 0.25 10 10 20 20 40 12 25 50 15 15 15 30 30 30 60 60 60 15 15 15 25 30 30 30 50 60 60 60 0.18 0.40 0.18 0.40 0.40 0.33 0.33 0.33 0.10 0.25 1.00 0.10 0.25 1.00 0.10 0.25 1.00 0.10 0.25 1.00 0.52 0.10 0.25 1.00 1.00 0.10 0.25 1.00 4 8 7 8 15 5 7 10 4 5 10 6 6 15 8 10 15 6 6 10 12 6 8 15 15 10 10 15 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 5,150 5,150 5,150 5,150 4,850 4,600 4,600 4,300 3,900 3,900 3,500 3,900 3,900 3,500 3,700 3,700 3,500 3,100 3,100 2,800 3,100 3,100 3,100 2,800 2,800 3,100 2,950 2,800 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 121-5012 121-5013 121-5022 121-5023 121-5043 128-5012 128-5022 128-5052 122-5011 122-5012 122-5013 122-5031 122-5032 122-5033 122-5061 122-5062 122-5063 123-5011 123-5012 123-5013 123-5026 123-5031 123-5032 123-5033 123-5053 123-5061 123-5062 123-5063 12 25 15 30 30 60 15 30 30 60 0.33 0.33 0.25 0.10 0.25 0.25 0.25 0.10 0.25 0.25 3 4 3 3 4 7 3.5 3 4 8 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 4,800 4,800 3,900 3,900 3,900 3,700 3,100 3,100 3,100 3,000 -60 to 340/360 -60 to 340/360 -60 to 340/360 -60 to 340/360 -60 to 340/360 -60 to 340/360 -60 to 340/360 -60 to 340/360 -60 to 340/360 -60 to 340/360 128-0112 128-0122 122-0112 122-0131 122-0132 122-0162 123-0112 123-0131 123-0132 123-0162 Length (m) Film Thickness (m) Maximum Allowable Bleed (pA) RI Tolerance (+/- I) Minimum Plates/ Meter Temperature Limits (C) Part No.
GC and GC/MS
0.25 0.25 0.25 0.25 0.25 0.25 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 DB-5ms 0.18 0.18 0.20 0.20 0.20 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.25
20 40 12 25 50 15 15 15 30 30 30 30 60 60 60
0.18 0.18 0.33 0.33 0.33 0.10 0.25 1.00 0.10 0.25 1.00 0.50 0.10 0.25 1.00
3 3 3 4 5 2.5 3 6 3 4 6 5 6 8.5 12
0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5
4,900 4,900 4,600 4,600 4,300 3,700 3,700 3,500 3,700 3,700 3,500 3,700 3,500 3,700 3,500
-60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350
121-5522 121-5542 128-5512 128-5522 128-5552 122-5511 122-5512 122-5513 122-5531 122-5532 122-5533 122-5536 122-5561 122-5562 122-5563
308
GC Columns
ID (mm) 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 DB-5ms ITD 0.25 DB-WAX 0.18 0.18 0.18 0.18 0.18 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.32 0.32 0.32 0.32 0.32 0.32 0.32 DB-17 0.18 0.18 0.18 0.18 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.32 0.32 0.32 0.32 0.32 0.32 10 10 20 20 15 15 15 30 30 30 60 15 15 15 30 30 30 0.18 0.30 0.18 0.30 0.15 0.25 0.50 0.15 0.25 0.50 0.25 0.15 0.25 0.50 0.15 0.25 0.50 5 10 10 15 10 10 15 10 10 15 15 10 10 15 10 10 15 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 4,850 4,500 4,850 3,950 3,700 3,700 2,900 3,700 3,700 2,900 3,500 3,000 3,000 2,650 3,000 3,000 2,650 40 to 280/300 40 to 280/300 40 to 280/300 40 to 280/300 40 to 280/300 40 to 280/300 40 to 280/300 40 to 280/300 40 to 280/300 40 to 280/300 40 to 280/300 40 to 280/300 40 to 280/300 40 to 280/300 40 to 280/300 40 to 280/300 40 to 280/300 121-1712 121-1713 121-1722 121-1723 122-1711 122-1712 122-1713 122-1731 122-1732 122-1733 122-1762 123-1711 123-1712 123-1713 123-1731 123-1732 123-1733 10 10 20 20 40 15 15 15 30 30 30 60 60 60 15 15 30 30 30 60 60 0.18 0.30 0.18 0.30 0.30 0.15 0.25 0.50 0.15 0.25 0.50 0.15 0.25 0.50 0.25 0.50 0.15 0.25 0.50 0.25 0.50 10 15 15 15 20 15 20 20 15 20 20 15 30 30 20 20 15 20 20 30 30 2.5 2.0 2.5 2.0 2.0 2.0 2.0 2.5 2.0 2.0 2.5 2.5 2.5 2.5 2.0 2.0 2.0 2.0 2.0 2.0 2.0 4,600 4,600 4,600 4,600 4,300 3,600 3,500 3,300 3,600 3,500 3,300 3,600 3,500 3,300 3,000 2,800 3,000 3,000 2,800 2,800 2,800 20 to 250/260 20 to 240/260 20 to 250/260 20 to 240/260 20 to 240/260 20 to 250/260 20 to 250/260 20 to 240/260 20 to 250/260 20 to 250/260 20 to 240/260 20 to 250/260 20 to 250/260 20 to 240/260 20 to 250/260 20 to 240/260 20 to 250/260 20 to 250/260 20 to 240/260 20 to 250/260 20 to 240/260 121-7012 121-7013 121-7022 121-7023 121-7043 122-7011 122-7012 122-7013 30 0.25 4 0.5 3,700 -60 to 325/350 122-4132 Length (m) 15 15 15 25 30 30 30 30 60 60 60 Film Thickness (m) 0.10 0.25 1.00 0.52 0.10 0.25 0.50 1.00 0.10 0.25 1.00 Maximum Allowable Bleed (pA) 2.5 3.5 6 5 3 4 5 6 6 9 12 RI Tolerance (+/- I) 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 Minimum Plates/ Meter 3,100 3,000 2,800 3,000 3,100 3,000 3,000 2,800 2,800 2,800 2,800 Temperature Limits (C) -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 Part No. 123-5511 123-5512 123-5513 123-5526 123-5531 123-5532 123-5536 123-5533 123-5561 123-5562 123-5563
DB-5ms (continued)
GC and GC/MS
122-7031 122-7032 122-7033 122-7061 122-7062 122-7063 123-7012 123-7013 123-7031 123-7032 123-7033 123-7062 123-7063
309
GC Columns
ID (mm) DB-17ms 0.18 0.25 0.25 0.25 0.32 0.32 DB-35ms 0.20 0.25 0.25 0.25 0.32 DB-200 0.25 0.25 0.25 0.32 0.32 DB-225ms 0.25 0.25 0.25 15 30 60 15 30 0.25 0.25 0.25 0.25 0.25 15 15 30 15 15 3 3 3 3 3 3,300 3,300 3,300 2,625 2,625 40 to 240 40 to 240 40 to 240 40 to 240 40 to 240 122-2912 122-2932 122-2962 123-2912 123-2932 15 30 30 30 30 0.25 0.25 0.50 0.25 0.50 10 10 15 10 15 1 1 1 1 1 3,500 3,500 3,500 2,800 2,800 30 to 300/320 30 to 300/320 30 to 300/320 30 to 300/320 30 to 300/320 122-2012 122-2032 122-2033 123-2032 123-2033 25 15 30 60 30 0.33 0.25 0.25 0.25 0.25 5 5.5 6 13 7.5 1 1 1 1 1 4,300 3,500 3,500 3,500 2,800 50 to 340/360 50 to 340/360 50 to 340/360 50 to 340/360 50 to 340/360 128-3822 122-3812 122-3832 122-3862 123-3832 20 15 30 60 15 30 0.18 0.25 0.25 0.25 0.25 0.25 5.5 5.5 5.5 11 7 7 1.5 1.0 1.0 1.0 1.0 1.0 3,700 3,700 3,700 3,500 2,800 2,800 40 to 320/340 40 to 320/340 40 to 320/340 40 to 320/340 40 to 320/340 40 to 320/340 121-4722 122-4712 122-4732 122-4762 123-4712 123-4732 Length (m) Film Thickness (m) Maximum Allowable Bleed (pA) RI Tolerance (+/- I) Minimum Plates/ Meter Temperature Limits (C) Part No.
GC and GC/MS
0.32 0.32 DB-XLB 0.18 0.18 0.20 0.20 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.32 0.32 0.32 0.32
20 30 12 25 15 15 30 30 30 30 60 30 30 30 60
0.18 0.18 0.33 0.33 0.10 0.25 0.10 0.25 1.00 0.50 0.25 0.25 1.00 0.50 0.25
6 6 5 6 4 6 4 6 9 9.5 12 7.5 13 9.5 15
1 1 1 1 1 1 1 1 1 1 1 1 1 1 1
5,150 5,150 4,600 4,600 3,700 3,900 3,700 3,900 3,500 3,700 3,700 3,000 2,800 3,100 3,000
30 to 340/360 30 to 340/360 30 to 340/360 30 to 340/360 30 to 340/360 30 to 340/360 30 to 340/360 30 to 340/360 30 to 340/360 30 to 340/360 30 to 340/360 30 to 340/360 30 to 340/360 30 to 340/360 30 to 340/360
121-1222 121-1232 128-1212 128-1222 122-1211 122-1212 122-1231 122-1232 122-1233 122-1236 122-1262 123-1232 123-1233 123-1236 123-1262
310
GC Columns
Fused Silica Tubing
Fused Silica Tubing

Deactivated Tubing
Deactivated tubing can be used as retention gaps, guard columns or transfer lines. Our standard deactivation process is a phenyl methyl deactivation the preferred choice for most applications due to its inertness and robustness.
Fused Silica, Deactivated

ID (mm) OD (mm) Length (m) Part No.
0.05 0.05 0.05 0.10 0.10 0.10 0.10 0.10 0.10 0.10 0.18 0.18 0.18 0.20 0.20 0.20 0.25 0.25 0.25 0.25 0.32 0.32 0.32 0.32 0.45 0.45 0.45 0.53 0.53 0.53 0.53
0.36 0.36 0.36 0.19 0.19 0.19 0.36 0.36 0.36 0.36 0.34 0.34 0.34 0.34 0.34 0.34 0.35 0.35 0.35 0.35 0.43 0.43 0.43 0.43 0.67 0.67 0.67 0.67 0.67 0.67 0.67
1 5 10 1 5 10 1 5 5 10 1 5 10 1 5 10 1 5 10 30 1 5 10 30 1 5 10 1 5 10 30
160-2655-1 160-2655-5 160-2655-10 160-1010-1 160-1010-5 160-1010-10 160-2635-1 160-2635-5 19091-60620E* 160-2635-10
GC and GC/MS
160-2615-1 160-2615-5 160-2615-10 160-2205-1 160-2205-5 160-2205-10 160-2255-1 160-2255-5 160-2255-10 160-2255-30** 160-2325-1 160-2325-5 160-2325-10 160-2325-30** 160-2455-1 160-2455-5 160-2455-10 160-2535-1 160-2535-5 160-2535-10 160-2535-30**
*Wound on a 5 in. cage **Wound on a 7 in. cage
311
GC Columns
Fused Silica Tubing
Fused Silica, High Temp (400C) Deactivated
0.05 0.10 0.18 0.25 0.25 0.32 0.32 0.53 0.53
0.36 0.36 0.34 0.35 0.35 0.43 0.43 0.67 0.67
5 5 5 5 10 5 10 5 10
160-2815-5 160-2825-5 160-2835-5 160-2845-5 160-2845-10 160-2855-5 160-2855-10 160-2865-5 160-2865-10
ProSteel Stainless Steel, Deactivated

0.53
0.67
160-4535-5
GC and GC/MS
312
GC Columns
Fused Silica Tubing Undeactivated Tubing
Undeactivated tubing or bare fused silica is commonly used for capillary electrophoresis. It can also be used for transfer lines and other applications where inertness is not critical.
Fused Silica, Undeactivated

0.02 0.02 0.05 0.05 0.075 0.10 0.10 0.18 0.18 0.20 0.20 0.20 0.25 0.25 0.32 0.32 0.32 0.45 0.45 0.53 0.53
0.36 0.36 0.36 0.36 0.36 0.36 0.36 0.34 0.34 0.34 0.34 0.34 0.35 0.35 0.43 0.43 0.43 0.67 0.67 0.67 0.67
5 10 5 10 10 5 10 5 10 5 10 50 5 10 5 10 50 5 10 5 10
160-2660-5 160-2660-10 160-2650-5 160-2650-10 160-2644-10 160-2634-5 160-2634-10 160-2610-5 160-2610-10 160-2200-5 160-2200-10 19091-20050*
GC and GC/MS
160-2250-5 160-2250-10 160-2320-5 160-2320-10 19091-21050* 160-2450-5 160-2450-10 160-2530-5 160-2530-10

*Wound on a 7 in. cage
Stainless Steel, Undeactivated

ID (mm) OD (mm) Length (ft) Part No.
0.76 (1/32 in.)
1.59 (1/16 in.)
50
700-0013-50
313
GC Columns
Column Installation and Tools

The Capillary Column Installation Kit contains: Diamond-tipped pencil 20x magnifier Ferrule removal tool Septum removal tool Refillable 2 oz. bottle of Snoop
Column Installation Kit P/N 430-2000

Description Column Installation Kit with Diamond Tip Pencil Part No. 430-2000
Column Rinse Kit

This kit makes solvent rinsing easy! Solvent rinsing removes many contaminants that accumulate in columns. The contaminants are removed by passing solvents through the column. Only bonded and cross-linked phase columns should be rinsed, or permanent damage to the column will result.
Column Rinse Kit

Description Capillary Rinse Kit Unit 10/pk Part No. 430-3000 0100-1375
GC and GC/MS
Column Rinse Kit P/N 430-3000
Replacement 1/16 in. Teflon Ferrule
Ceramic Wafers
For a full listing of cutting tools see page 88.
20x Magnifier
Examine column ends for proper cuts. Jagged, rough, or non-perpendicular cuts negatively affect chromatographic performance.
Column Ferrule Installation Tool

Tools
Column Ferrule Installation Tool P/N 19251-80680
Description Ceramic Wafer 20x Magnifier Column Ferrule Installation Tool
Unit 4/pk
Part No. 5181-8836 430-1020 19251-80680
314
GC Columns
Stationary Phase Applications Guide
Method/Column Selection Guides

GC Columns Stationary Phase Applications Guide
GC Columns Stationary Phase Applications Guide Approximate Temperature Range (C) Phases With (Isothermal/Programmed)*Similar Selectivity
Agilent Phase
Composition
Polarity
General Applications
HP-1ms, DB-1ms, Amines, hydrocarbons, HP-1, DB-1 pesticides, PCBs, phenols, sulfur compounds, flavors and fragrances HP-5ms, DB-5, HP-5
100% Dimethylpolysiloxane
Non-polar
From -60 to 325/350
BP-1, SPB-1, CP-Sil 5, Rtx-1, OV-1, SE-30, 007-1, ZB-1 SPB-5, XTI-5, Mtx-5, CP-Sil 8CB, SE-54, Rtx-5, BPX-5, MDN-5, Rtx-5ms, BP-5, ZB-5
Semivolatiles, alkaloids, 5% Phenyl 95% drugs, FAMEs, halogenated dimethylpolysiloxane compounds, pesticides, herbicides
Non-polar
From -60 to 325/350
DB-5ms
Semivolatiles, alkaloids, 5% Phenyl drugs, FAMEs, halogenated 95% dimethyl compounds, pesticides, arylene siloxane herbicides Aroclors, alcohols, pesticides, VOCs CLP-pesticides, aroclors, pharmaceuticals, drugs of abuse CLP-pesticides, aroclors, pharmaceuticals, drugs of abuse Pesticides, herbicides, TMS sugars, aroclors Drugs, glycols, pesticides, steroids Drugs, glycols, pesticides, steroids 6% Cyanopropyl-phenyl Mid-polar 94% dimethyl polysiloxane 35% Phenyl 65% dimethyl Mid-polar polysiloxane 35% Phenyl 65% dimethyl arylene siloxane 14% Cyanopropyl-phenyl Mid-polar 86% dimethyl polysiloxane
From -60 to 325/350
SPB-5, XTI-5, Mtx-5, CP-Sil 8CB, SE-54, Rtx-5, BPX-5, MDN-5, Rtx-5ms Rtx-1301, Mtx-1301, CP-130 1 Rtx-35, SPB-35, AT-35, Sup-Herb, MDN-35, BPX-35 Rtx-35, SPB-35, AT-35, Sup-Herb, MDN-35, BPX-35 SPB-1701, CP-Sil 19 CB, Rtx-1701, CB-1701, OV-1701, 007-1701, BPX-10 Rtx-50, CP-Sil 19 CB, BPX-50, SP-2250
DB-1301 DB-35, HP-35
From -20 to 280/300 From 40 to 300/320
DB-35ms
From 50 to 340/360
GC and GC/MS
DB-1701, DB-1701P HP-50+, DB-17 DB-17ms
From -20 to 280/300
DB-200 DB-210 DB-225ms, DB-225 HP-INNOWax
50% Phenyl Mid-polar 50% dimethylpolysiloxane 50% Phenyl 50% dimethyl arylene siloxane Residual solvents, pesticides, 35% Trifluoropropyl Polar herbicides 65% dimethyl polysiloxane 50% Trifluoropropyl 50% dimethyl polysiloxane FAMEs, alditol acetates, 50% Cyanopropyl-phenyl Polar neutral sterols 50% dimethyl polysiloxane Polar
From 40 to 280/300 From 40 to 320/340
From 30 to 300/320 From 45 to 240/260 From 40 to 220/240
Rtx-200
Alcohols, free organic acids, Polyethylene glycol solvents, essential oils, flavors and fragrances DB-WAX Solvents, glycols, alcohols Polyethylene glycol CAM Amines, basic compounds Polyethylene glycol-base modified HP-FFAP, DB-FFAP Organic acids, alcohols, Polyethylene glycolaldehydes, ketones, acid modified acrylates DB-23 FAMEs (requiring 50% Cyanopropyl cis/trans resolution) 50% dimethyl polysiloxane CycloSil-B Chiral compounds 30%-heptakis (general purpose) (2,3-di-O-methyl-6-O-t-butyl dimethylsilyl)-B-cyclodextrin in DB-1701 HP-Chiral Chiral compounds (using a beta-Cyclodextrin in phenylColumns Nitrogen selective detector, based stationary phase NPD)
From 40 to 260/270
Polar Polar Polar
From 20 to 250/260 From 60 to 220/240 From 40 to 250
Polar Mid-polar
From 40 to 250/260 From 35 to 260/280
SP-2330, CP-Sil 43 CB, OV-225, Rtx-225, BP-225, 007-225 BP-20, 007-CW, CP-WAX 52 CB, Stabilwax, Supelcowax-10 Rt-Wax Carbowax Amine, Stabilwax-DB, CP-51 WAX OV-351, SP-1000, Stabilwax-DA, 007-FFAP, Nukol Rtx-2330, 007-23, SP-2330/2340/2380/2560 LIPODEX C, Rt-BDEXm, B-DEX 110, B-DEX 120
Mid-polar
From 30 to 240/250
LIPODEX C, Rt-BDEXm, B-DEX 110, B-DEX 122
315
GC Columns
Stationary Phase Applications Guide
GC Columns Stationary Phase Applications Guide (continued)
Approximate Temperature Range (C) Phases With (Isothermal/Programmed)*Similar Selectivity
Agilent Phase
Composition
Polarity
PLOT Column Applications
GC and GC/MS
HP-PLOT Molesieve Permanent and noble gases. Argon and oxygen separation at 35C HP-PLOT C1-C6 hydrocarbons in Al2O3 KCl natural gas, refinery gas, fuel gas, synthetic gas, dienes HP-PLOT C1-C6 hydrocarbons in Al2O3 S natural gas, refinery gas, fuel gas, synthetic gas, dienes GS-Alumina C1-C6 hydrocarbons in natural gas, refinery gas, fuel gas, synthetic gas, dienes HP-PLOT Q Hydrocarbons including isomers, CO2, methane, air/CO, water, polar solvents, sulfur compounds HP-PLOT U C1 to C7 hydrocarbons, CO2, methane, air/CO, water, oxygenates, amines, solvents, alcohols, ketones, aldehydes GS-GasPro C1 to C12 hydrocarbons, CO2, trace-level sulfurs, hydride gases, inorganic gases, halocarbons, SF6, oxygen/nitrogen separation at 80C GS-CarbonPLOT C1 to C5 hydrocarbons, CO2, air/CO, trace acetylene in ethylene, methane
Specialty Phases - Environmental Applications
5 molecular sieve zeolite
From -60 to 300
Rt-Molesieve 13X, Molesieve 5 AluminaPlot, Rt-Alumina, CP-Al2O3/KCl Plot
Aluminum Oxide "KCl" deactivated Aluminum Oxide "Sodium Sulfate" deactivated Aluminum Oxide with proprietary deactivation Polystyrene-divinylbenzene
Least polar
From -60 to 200
Mid-polar
From -60 to 200
Most polar
From -60 to 200
From -60 to 270/290
PoraPlot Q/S, Rt-Q, Supel-Q PLOT
Divinylbenzene/ethylene glycol dimethacrylate
From -60 to 190
PoraPlot U
Proprietary, bonded silica-based
From -80 to 260/300
CP-SilicaPLOT
Bonded monolithic carbon layer
From 0 to 360
CP-CarboBond
DB-624 DB-VRX DB-35ms
DB-XLB (Confirmation Column) HP-5ms
6% Cyanopropyl-phenyl Mid-polar 94% dimethyl polysiloxane Volatile Organic Compounds Proprietary phase Non-polar using MSD, ELCD/PID CLP Pesticides, Chlorinated 35% Phenyl Mid-polar Herbicides, PCBs, 65% dimethyl 508.1 Pesticides arylene siloxane Proprietary phase Non-polar
From -20 to 260C From -10 to 260C From 50 to 340/360C
Rtx-624, AT-624, SPB-624, CP-624, PE-624,007-624 Rtx-VRX MDN-35, BPX-35
From 30 to 340/360C
No equivalent
Semivolatiles by EPA Method 8270 PCB Congener Analysis (209 Congeners) CLP Pesticides, Chlorinated Herbicides, PCBs, 508.1 Pesticides Leaking Underground Fuel Tank (LUFT) testing MTBE in Soil and Water
5% Phenyl 95% dimethylpolysiloxane Proprietary phase
Non-polar
DB-XLB
Non-polar
From -60 to 325/350C SPB-5, XTI-5, Mtx-5, CP-Sil 8CB, SE-54, Rtx-5, BPX-5, MDN-5, Rtx-5ms From 30 to 340/360C
DB-TPH DB-MTBE
Proprietary phase Proprietary phase
Non-polar Non-polar
From -10 to 290C From 35 to 260/280 From -20 to 260C
Other Specialty Phases
HP-Fast GC Residual Solvents Residual Solvent Column DB-ALC1 Blood Alcohol Testing DB-ALC2 HP-Blood Alcohol Column
6% Cyanopropyl-phenyl Mid-polar 94% dimethyl polysiloxane Proprietary phase Proprietary phase Proprietary phase Mid-polar Mid-polar
From 20 to 260/280C From 20 to 260/280C From -60 to 270/290
*Temperature limits vary with column dimensions. Always confirm temperature limits prior to column use.
316
GC Columns
Environmental/EPA Methods Environmental/EPA Methods
Many possible column and instrument combinations can be used to obtain successful Environmental and EPA Analyses. Listed below are a few of the columns Agilent would recommend for these analyses. The following recommendations are based upon GCs equipped with split/splitless injectors (except for the volatiles methods). Other column configurations may be suitable with different instrument configurations. To tailor your analytical system to your particular needs, contact your local Aglilent office for the best column recommendation.
Environmental/EPA Methods
Analyte Type
Volatiles
EPA Method Common Sample Reference Preparation 501 Purge and trap, direct injection, headspace Purge and trap, direct injection, headspace
Detector Types ELCD, ECD
Sample Matrix Drinking water
Agilent Column Recommendation (Part No.) DB-VRX, 30 m x 0.45 mm x 2.55 m (124-1534) DB-624, 30 m x 0.45 mm x 2.55 m (124-1334) DB-VRX, 75 m x 0.45 mm x 2.55 m (124-1574) DB-624, 75 m x 0.45 mm x 2.55 m (124-1374) DB-VRX, 75 m x 0.45 mm x 2.55 m (124-1574) DB-624, 75 m x 0.45 mm x 2.55 m (124-1374) DB-VRX, 30 m x 0.45 mm x 2.55 m (124-1534) DB-624, 30 m x 0.45 mm x 2.55 m (124-1334) DB-VRX, 60 m x 0.25 mm x 1.4 m (122-1564) DB-624, 60 m x 0.25 mm x 1.4 m (122-1364) HP-VOC, 60 m x 0.20 mm x 1.1 m (19091R-306) DB-VRX, 20 m x 0.18 mm x 1.0 m (121-1524) DB-624, 20 m x 0.18 mm x 1.0 m (121-1324) DB-VRX, 30 m x 0.45 mm x 2.55 m (124-1534) DB-624, 30 m x 0.45 mm x 2.55 m (124-1334) DB-VRX, 30 m x 0.45 mm x 2.55 m (124-1534) DB-624, 30 m x 0.45 mm x 2.55 m (124-1334) HP-5ms, 30 m x 0.25 mm x 0.5 m (19091S-133)
Trihalomethanes
Volatile Organic Compounds (VOCs)
502.2, 8021, CLP-Volatiles
PID, ELCD
Purgeable 601, 8010 Halogenated Organics Purgeable Aromatic Organics Volatile Organic Compounds (VOCs) Using MSD Volatile Organic Compounds (VOCs) Using 5973 MSD EDB and DBCP Acrylonitrile and Acrolein
Semivolatiles
Purge and trap, PID, ELCD headspace for screening Purge and headspace for screening Purge and trap, direct injection, headspace Purge and trap, direct injection, headspace Microextraction with Hexane Purge and trap, liquid extraction, sonication Liquid extraction, sonication, soxhlet extraction, SPE Liquid extraction, sonication, soxhlet extraction, derivatization Liquid extraction, sonication, soxhlet extraction, SPE Liquid extraction PID
Drinking water, waste water, solid wastes Waste water, solid wastes
503.1, 602, 8020 524.2, 624, 8240, 8260, CLP-VOCs 524.2, 624, 8240, 8260, CLP-VOCs 504.1, 8011 603, 8015, 8031 525, 625, 8270 528, 604, 8040, 8041
Drinking water, trap, waste water, solid wastes MSD Drinking water, waste water, solid wastes MSD (5973) Drinking water, waste water, solid wastes ECD Drinking water, solid wastes FID, NPD Waste water, solid wastes MSD Drinking water, waste water, solid wastes Waste water, solid wastes
GC and GC/MS
Semivolatile Organic Compounds Phenols
ECD, FID
Phthalate Esters
506, 606, 8060, 8061 605
ECD, FID
Benzidines
ECD
Drinking water, waste water, solid wastes Waste water
Nitrosamines
607, 8070
Nitroaromatics and Isophorone Polynuclear Aromatic Hydrocarbons (PAHs) Chlorinated Hydrocarbons
609, 8090
610, 8100
612, 8120, 8121
Chlorinated Disinfection 551, 551.1A Byproducts Halogenated 552, 552.1, Acetic Acids 552.2
Liquid extraction, sonication, soxhlet extraction, SPE Liquid extraction, sonication, soxhlet extraction, SPE Liquid extraction, sonication, soxhlet extraction, SPE Liquid extraction, sonication, soxhlet extraction, SPE Liquid extraction, derivatization Liquid extraction, derivatization
NPD
Waste water, solid wastes Waste water, solid wastes Waste water, solid wastes Waste water, solid wastes Drinking water Drinking water
DB-5ms, 30 m x 0.25 mm x 0.25 m (122-5532) DB-XLB, 30 m x 0.25 mm x 0.25 m (122-1232) DB-5ms, 30 m x 0.53 mm x 1.5 m (125-5532) DB-608, 30 m x 0.53 mm x 0.5 m (125-6837) DB-5ms, 30 m x 0.25 mm x 0.25 m (122-5532) DB-5ms, 30 m x 0.53 mm x 1.5 m (125-5532) DB-608, 30 m x 0.53 mm x 0.5 m (125-6837) DB-5ms, 30 m x 0.25 mm x 0.25 m (122-5532) DB-5ms, 30 m x 0.53 mm x 1.5 m (125-5532) DB-608, 30 m x 0.53 mm x 0.5 m (125-6837) DB-5ms, 30 m x 0.25 mm x 0.25 m (122-5532) DB-5ms, 30 m x 0.53 mm x 1.5 m (125-5532) HP-5ms, 30 m x 0.25 mm x 0.5 m (19091S-133) DB-5ms, 30 m x 0.53 mm x 1.5 m (125-5532) DB-608, 30 m x 0.53 mm x 0.5 m (125-6837) DB-5ms, 30 m x 0.25 mm x 0.25 m (122-5532) DB-5ms, 30 m x 0.32 mm x 0.25 m (123-5532) DB-1ms, 30 m x 0.25 mm x 0.25 m (122-0132) DB-5ms, 30 m x 0.32 mm x 0.5 m (123-5536) HP-5ms, 30 m x 0.32 mm x 0.5 m (19091S-113) DB-1, 30 m x 0.32 mm x 0.5 m (123-103E) DB-5ms, 30 m x 0.25 mm x 1.0 m (122-5533) DB-1, 30 m x 0.25 mm x 1.0 m (122-1033) DB-35ms, 30 m x 0.32 mm x 0.25 m (123-3832) DB-XLB, 30 m x 0.32 mm x 0.5 m (123-1236)
ECD, FID
FID
ECD
ECD ECD
317
GC Columns
ASTM Method Guide
Environmental/EPA Methods (continued)
Analyte Type Organochlorine Pesticides and PCBs Phenoxy Acid Herbicides N- and P-Containing Pesticides and Herbicides PCB Congeners Using MSD EPA Method Common Sample Reference Preparation 508.1, 608, 8081A, 8082, CLP-Pesticides 515, 615, 8150, 8151 507, 614, 619, 622, 8140, 8141A Liquid extraction, sonication, soxhlet extraction, SPE Liquid extraction, sonication, soxhlet extraction, SPE Liquid extraction, sonication, soxhlet extraction, SPE Detector Types ECD Sample Matrix Agilent Column Recommendation (Part No.) DB-35ms, 30 m x 0.32 mm x 0.25 m (123-3832) DB-XLB, 30 m x 0.32 mm x 0.5 m (123-1236) DB-35ms, 30 m x 0.32 mm x 0.25 m (123-3832) DB-XLB, 30 m x 0.32 mm x 0.5 m (123-1236) DB-35ms, 30 m x 0.25 mm x 0.25 m (122-3832) DB-5ms, 30 m x 0.25 mm x 0.25 m (122-5532) DB-XLB, 30 m x 0.18 mm x 0.18 m (121-1232) DB-XLB, 60 m x 0.25 mm x 0.25 m (122-1262)
Pesticides, Herbicides and PCBs
Drinking water, waste water, solid wastes ECD Drinking water, waste water, solid wastes NPD, ELCD, Drinking water, FPD waste water, solid wastes MSD
ASTM
ASTM Methods
Method Designation D 1945 Method Title Standard Test Method for the Analysis of Natural Gas by GC Standard Test Method for the Analysis of Reformed Gas by GC Standard Test Method for Fatty Acid Composition by Gas-Liquid Chromatography of Methyl Esters Standard Test Method for the Analysis of Liquified Petroleum (LP) Gases and Propene Concentrates by GC Column Recommendation HP-Molesieve, 15 m x 0.53 mm id, 50 m HP-PLOT Q, 15 m x 0.53 mm id, 40 m D 1946 HP-Molesieve, 15 m x 0.53 mm id, 50 m HP-PLOT Q, 15 m x 0.53 mm id, 40 m D 1983 DB-WAX, 30 m x 0.25 mm id, 0.25 m film Part No. 19095P-MS9 19095P-QO3 19095P-MS9 19095P-QO3 122-7032
GC and GC/MS
D 2163
HP-Alumina/KCl, 30 m x 0.53 mm id (Agilent preferred) HP-Alumina S, 30 m x 0.53 mm id (Agilent preferred alternate)
19095P-K23
19095P-S23
D 2268 122-106E D 2306 D 2426
Standard Test Method for Analysis of High-Purity n-Heptane and Isooctane by Capillary GC Standard Test Method fo C8 Aromatic Hydrocarbons by GC Standard Test Method for Butadiene Dimer and Styrene in Butadiene Concentrates by GC Standard test Method for Determination of C2 through C5 Hydrocarbons in Gasoline by GC Standard Test Method for Noncondensable Gases in C2 and Lighter Hydrocarbon Products by GC Standard Test Method for Ethylene, Other Hydrocarbons, and Carbon Dioxide in High-Purity Ethylene by GC Standard Test Method for Butadiene Purity and Hydrocarbon Impurities by GC Standard Test Method for Hydrocarbon Traces in Propylene Concentrates by GC Standard Test Method for Purity of Methyl Ethyl Ketone by GC Standard Test Method for Boiling Range Distribution of Petroleum Fractions by GC Standard Test Method for Boiling Range Distribution of Petroleum Fractions by GC, to C60
DB-1, 60 m x 0.25 mm id, 0.50 m film (Agilent preferred) HP-INNOWax, 60 m x 0.25 mm id, 0.25 m film (Agilent preferred) DB-1, 30 m x 0.53 mm id, 5.00 m film (Agilent preferred) DB-1, 30 m x 0.53 mm id, 5.0 m film (Agilent preferred) GS- Alumina, 30 m x 0.53 mm id (Agilent preferred) 19091N-136 125-1035
D 2427
125-1035
115-3532 19095P-MS0
D 2504
HP-Molesieve, 30 m x 0.53 mm id, 50 m film
D 2505
GS-GasPro, 60 m x 0.32 mm id (Agilent preferred)
113-4362
D 2593 D 2712 D 2804
GC-Alumina, 30 m x 0.53 mm id (Agilent preferred) GS-Alumina, 50 m x 0.53 mm id (Agilent preferred) DB-WAX, 30 m x 0.53 mm id, 1.0 m film (Agilent preferred) DB-210, 15 m x 0.53 mm id, 1.0 m film DB-2887, 10 m x 0.53 mm id, 3.00 m film
115-3532 115-3552 125-7032 125-0212 125-2814
D 2887
"Extended D 2887"
HP-1, 10 m x 0.53 mm id, 0.88 m film
19095Z-021
HP-1, 5 m x 0.53 mm id, 0.88 m film
19095Z-020
318
GC Columns
ASTM Method Guide
ASTM Methods (continued)
Method Designation D 2908 Method Title Standard Practice for Measuring Volatile Organic Matter in Water by Aqueous-Injection GC Standard Test Method for Analysis of Cyclohexane by GC Standard Test Method for Aromatics in Mineral Spirits by GC Standard Test Method for Purity of Methyl Isobutyl Ketone by GC Column Recommendation Contact Agilent for recommendation Part No.
D 3054 D 3257 D 3329
DB-1, 60 m x 0.32 mm id, 0.50 m film DB-624, 30 m x 0.53 mm id, 3.0 m film (Agilent preferred) DB-WAX, 30 m x 0.53 mm id, 1.0 m film (Agilent preferred) DB-624, 30 m x 0.45 mm id, 2.55 m film (Agilent preferred)
123-106E 125-1334 125-7032 124-1334 19091S-713
D 3432
Standard Test Method for Unreacted Toluene Diisocyanates in Urethane Prepolymers and Coating Solutions by GC Standard Test Method for Purity of Halogentated Organic Solvents
HP-1ms, 30 m x 0.32 mm id, 1.00 m film
D 3432 D 3447 D 3534 D 3545 D 3687
DB-1, 30 m x 0.45 mm id, 0.42 m film DB-624, 30 m x 0.53 mm id, 3.0 m film (Agilent preferred)
124-1037 125-1334 124-1232 125-1334 125-7032
Standard Test Method for PCBs in Water DB-XLB, 30 m x 0.45 mm id, 1.27 m film (Agilent preferred) Standard Test Method for Alcohol DB-624, 30 m x 0.45 mm id, 2.55 m film Content and Purity of Acetate Esters by GC (Agilent preferred) Standard Practice for Analysis of Organic DB-WAX, 30 m x 0.53 mm id, 1.0 m film Vapors Collected by the Activated Charcoal Tube Adsorption Method DB-WAX, 30 m x 0.45 mm id, 0.85 m film Standard Test Method for Volatile Alcohols in Water by Direct AqueousInjection GC DB-WAX, 30 m x 0.53 mm id, 1.0 m film (Agilent preferred)
124-7032 125-7032
D 3695
D 3710
Standard Test Method for Boiling Range DB-2887, 10 m x 0.53 mm id, 3.00 m film Distribution of Gasoline and Gasoline Fractions by GC Standard Test Method for Analysis of Isopropylbenzene (Cumene) by GC Standard Test Method for Analysis of o-Xylene by GC Standard Test Method for Analysis of p-Xylene by GC Standard Test Method for Purgeable Organic Coumpounds in Water Using Headspace Sampling Standard Test Method for Purity of Methyl Amyl Ketone and Methyl Isoamyl Ketone by GC
125-2814
GC and GC/MS
D 3760
DB-WAX 60 m x 0.32 mm id, 0.25 m film (Column A) 123-7062 HP-1, 50 m x 0.32 mm id, 0.52 m film (Column B) 19091Z-115 19091N-216 19091N-216 124-1574
D 3797 D 3798 D 3871
HP-INNOWax, 60 m x 0.32 mm id, 0.50 m film HP-INNOWax, 60 m x 0.32 mm id, 0.50 m film DB-VRX, 75 m x 0.45 mm id, 2.55 m film (Agilent preferred) DB-624, 30 m x 0.53 mm id, 3.0 m film (Agilent preferred)
D 3893
125-1334
D 3973 D 4415 D 4424 D 4443
Standard Test Method for Low-Molecular DB-VRX, 30 m x 0.45 mm id, 2.55 m film Weight Halogenated Hydrocarbons in Water (Agilent preferred) Standard Test Method for Determination DB-FFAP, 30 m x 0.32 mm id, 0.25 m film of Dimer in Acrylic Acid Standard Test Method for Butylene Analysis by GC HP-AluminaS, 50 m x 0.53 mm id (Agilent preferred)
124-1534 123-3232 19095P-S25 124-1534
Standard Test Method for Residual Vinyl DB-VRX, 30 m x 0.45 mm id, 2.55 m film Chloride Monomer Content in PPB Range (Agilent preferred) in Vinyl Chloride Homo- and Co-Polymers by Headspace GC Standard Test Method for Determination of DB-FFAP, 30 m x 0.45 mm id, 0.85 m film Trace Thiophene in Refined Benzene by GC Standard Test Method for Propylene DB-5, 10 m x 0.32 mm id, 5.0 m film Gycol Monomethyl Ether, Dipropylene Glycol Monomethyl Ether, and Propylene Glycol Monmethyl Ether Acetate DB-5, 30 m x 0.45 mm id, 4.25 m film (Agilent preferred)
D 4735
124-3232
D 4773
100-2000
124-5035 124-7032
D 4864
Standard Test Method for Determination DB-WAX, 30 m x 0.45 mm id, 0.85 m film of Traces of Methanol in Propylene Concentrates by GC
319
GC Columns
ASTM Method Guide
Method Designation D 4947 Method Title Standard Test Method for Chlordane and Heptachlor Residues in Indoor Air Column Recommendation DB-5, 30 m x 0.53 mm id, 1.5 m film DB-608, 30 m x 0.53 mm id, 0.83 m film (confirmation column, Agilent preferred) D 4961 Standard Test Method for GC Analysis of Major Organic Impurities in Phenol Produced by the Cumene Process Standard Test Method for Cyclohexylamine, Morpholine and Diethylaminoethanol in Water and Condensed Steam by Direct Aqueous Injection GC Part No. 125-5032 125-1730
DB-FFAP, 30 m x 0.45 mm id, 0.85 m film (Method A) 124-3232
HP-PLOT Q 15 m x 0.53 mm id (Method B) D 4983 HP-5ms 30 m x 0.32 mm id, 1.00 m film (Agilent preferred)
19095P-QO3 19091S-213
CAM, 30 m x 0.53 mm id, 1.00 m film (alternate column) D 5008 Standard Test Method for Ethyl Methyl Pentonal Content and Purity Value of 2-Ethylhexanol by GC HP-1, 15 m x 0.53 mm id, 5.0 m film
115-2132 19095Z-621
HP-INNOWax, 30 m x 0.32 mm id, 0.25 m film D 5060 D 5075 Standard Test Method for Determining HP-INNOWax, 60 m x 0.32 mm id, 0.50 m film Impurities in High-Purity Ethylbenzene by GC Standard Test Method for Nicotine in Indoor Air Standard Test Method for Detailed Analysis of Petroleum Naphthas Through n-Nonane by Capillary GC Standard Test Method for Analysis of Styrene by Capillary GC DB-5, 30 m x 0.53 mm id, 1.5 m film DB-5, 30 m x 0.32 mm id, 1.0 m film D 5134 HP-PONA, 50 m x 0.20 mm id, 0.50 m film
19091N-113 19091N-216 125-5032 123-5033 19091S-001
D 5135
HP-INNOWax, 60 m x 0.32 mm id, 0.50 m film
19091N-216 123-1033
GC and GC/MS
D 5175
Standard Test Method for Organohalide DB-1, 30 m x 0.32 mm id, 1.0 m film (Column 1) Pesticides and Polychlorinated Biphenyls in Water by Microextraction and GC DB-608, 30 m x 0.32 mm id, 0.5 m film (Column 3) DX-3, 30 m x 0.32 mm id, 0.25 m film (Column 2) DB-XLB, 30 m x 0.25 mm id, 0.25 m film (Agilent preferred)
123-1730 123-6332 122-1232 113-4332 19095P-QO4 19095Z-627
D 5303
Standard Test Method for Trace Carbonyl GS-GasPro, 30 m x 0.32 mm id (Agilent preferred) Sulfide in Propylene by GC HP-PLOT Q 30 m x 0.53 mm id, 40 m Standard Test Method for Determination HP-1, 7.5 m x 0.53 mm id, 5.0 m film of Boiling Range Distribution of Crude (Agilent preferred) Petroleum by GC Standard Test Method for Tar Acid Composition by Capillary GC HP-5ms, 30 m x 0.25 mm id, 0.25 m film DB-225ms, 30 m x 0.25 mm id, 0.25 m film (recommended)
D 5307
D 5310
19091S-433 122-2932 19091S-713
D 5316
Standard Test Method for 1, 2-Dibromoethane and 1, 2-Dibromo-3-Chloropropane in Water by Microextraction and GC
HP-1ms, 30 m x 0.32 mm id,1.00 m film (Column B)
DB-624, 30 m x 0.45 mm id, 2.55 m film (Agilent preferred Column B alternative) DX-3, 30 m x 0.32 mm id, 0.25 m film (Column A) D 5317 Standard Test Method for HP-5ms 30 m x 0.25 mm id, 0.25 m film (Column 1) Determination of Chlorinated Organic Acid Coumpounds in Water by GC with Electron Capture Detector DB-XLB, 30 m x 0.25 mm id, 0.25 m film (Agilent preferred) DB-35ms 30 m x 0.25 mm id, 0.25 m film (Agilent preferred) D 5320 Standard Test Method for Determination DB-1 30 m x 0.53 mm id, 3.0 m film of 1,1-Trichloroethane and Methylene Chloride in Stabilized Trichloroethylene and Tetrachloroethylene DB-VRX, 30 m x 0.32 mm id, 1.80 m film (Agilent preferred)
124-1334 123-6332 19091S-433
DB-1701P, 30 m x 0.25 mm id, 0.25 m film (Column 2) 122-7732 122-1232 122-3832 125-1034
123-1534
320
GC Columns
ASTM Method Guide
Method Designation D 5399 D 5441 Method Title Column Recommendation Standard Test Method fo Boiling Point DB-2887, 10 m x 0.53 mm id, 3.00 m film Distribution of Hydrocarbon Solvents by GC Standard Test Method for Analysis of Methyl Tert-Butyl Ether (MTBE) by GC Standard Test Method for Analysis of Petroleum Waxes by GC Standard Test Method for Nitrogenand Phosphorus-Containing Pesticides in Water by GC with a NitrogenPhosphorus Detector HP-PONA, 50 m x 0.20 mm id, 0.50 m film DB-Petro, 100 m x 0.25 mm id, 0.50 m film D 5442 DB-1, 25 m x 0.32 mm id, 0.25 m film DB-5, 15 m x 0.25 mm id, 0.25 m film D 5475 HP-5ms, 30 m x 0.25 mm id, 0.25 film (recommended in method) Part No. 125-2814 19091S-001 122-10A6 123-1022 122-5012 19091S-433
DB-1701P, 30 m x 0.25 mm id, 0.25 m film (recommended in method) DB-XLB, 30 m x 0.25 mm id, 0.25 m film (Agilent preferred) DB-35ms 30 m x 0.25 mm id, 0.25 m film (Agilent preferred) D 5480 D 5501 Standard Test Method for Engine Oil Volatility by GC DB-PS1 15 m x 0.53 mm id, 0.15 m film (Agilent preferred)
122-7732 122-1232 122-3832 145-1011 19091Z-530
Standard Test Method for Determination HP-1, 100 m x 0.25 mm id, 0.50 m film of Ethanol Content of Denatured Fuel Ethanol by GC Standard Test Method for Determination HP PLOT Q, 15 m x 0.53 mm id of Trace Organic Impurities in Monomer Grade Vinyl Chloride by Capillary Column/Multi-dimensional GC HP PLOT U, 30 m x 0.53 mm id Standard Test Method for Determination HP PLOT Q, 30 m x 0.53 mm id of Residual Acrylonitirle Monomer in Styrene-Acrylonitirle Co-poplymer Resins and Nitrile-Butadiene Rubber by Headspace-Capillary GC Standard Test Method for Determination DB-1, 30 m x 0.53 mm id, 5.0 m film of Benzene, Toluene, Ethylbenzene, (capillary column) p/m-Xylene, C9 and Heavier Aromatics, and Total Aromatics in Finished Gasoline by GC Standard Test Method for Determination HP-1, 60 m x 0.25 mm id, 1.0 m film of Oxygenates in Gasoline by GC and Oxygen Selective Flame Ionization Detection Standard Test Method for Sulfur Compounds in Light Petroleum Liquids by GC and Sulfur Selective Detection HP-1, 30 m x 0.32 mm id, 4.0 m film
D 5507
19095P-QO3
19095P-UO4 19095P-QO4
D 5508
GC and GC/MS
D 5580
125-1035
D 5599
19091Z-236
D 5623
19091Z-613
D 5713
Standard Test Method for Analysis of High DB-PETRO, 50 m x 0.20 mm id, 0.50 m film Purity Benzene for Cyclohexane Feedstock by Capillary GC Standard Practice for Oil Spill Source Identification by GC and Positive Ion Electron Impact Low Resolution Mass Spectrometry DB-5, 30 x 0.25 mm id, 0.25 m film
128-1056
D 5739
122-5032
DB-TPH, 30 m x 0.25 mm id, 0.25 m film D 5769 Standard Test Method for Determination HP-1, 60 m x 0.25 mm id, 1.0 m film of Benzene, Toluene, and Total Aromatics in Finished Gasoline by GC/MS Standard Test Method for Measurement DB-VRX, 60 m x 0.25 mm id, 1.40 m film of Purgeable Organic Compunds in Water (for 5972 and older MSDs) by Capillary Column GC/MS DB-VRX, 20 m x 0.18 mm id, 1.0 m film (for 5973 MSD) DB-624, 60 m x 0.25 mm id, 1.40 m film (for 5972 and older MSDs) DB-624, 20 m x 0.18 mm id, 1.0 m film (for 5973 MSD)
123-1632 19091Z-236
D 5790
122-1564
121-1524 122-1364 121-1324
321
GC Columns
ASTM Method Guide
Method Designation D 5812 Method Title Column Recommendation Standard Test Method for Determination HP-5ms, 30 m x 0.25 mm id, 0.25 m film of Organochlotine Pesticides in Water (recommended in method) by Capillary Column GC DB-1701P, 30 m x 0.25 mm id, 0.25 m film (recommended in method) DB-XLB, 30 m x 0.25 mm id, 0.25 m film (Agilent preferred) DB-35ms 30 m x 0.25 mm id, 0.25 m film (Agilent preferred) D 5917 Standard Test Method for Trace Impurities HP-INNOWax, 60 m x 0.32 mm id, 0.25 m film in Monocyclic Aromatic Hydrocarbons by GC and External Calibration Standard Test Methods for Fatty and Rosin Acids in Tall Oil Fraction Products by Capillary GC DB-23, 60 m x 0.25 mm id, 0.25 m film Part No. 19091S-433
122-7732 122-1232 122-3832 19091N-116
D 5974
122-2362
D 5986
Standard Tests Method for Determination HP-1, 60 m x 0.53 mm id, 5.0 m film of Oxygenates, Benzene, Toluene, C8-C12 Aromatics and Total Aromatics in Finished Gasoline by GC/FTIR Standard Test Method for Trace Impurities HP-1, 60 m x 0.25 mm id, 1.0 m film in Alpha-Methylstyrene by Capillary GC
19095Z-626
D 6144 D 6159
19091Z-236
Standard Test Method for Determination of HP-PLOT Alumina/KCl, 50 m x 0.53 mm id (Column 1) 19095P-K25 Hydrocarbon Impurities in Ethylene by GC GS-Alumina, 50 m x 0.53 mm id (Column 1, Agilent preferred) DB-1, 30 m x 0.53 mm id, 5.0 m film (Column 2) 115-3552 125-1035 19091S-413 122-1232 145-1001
D 6160
Standard Test Method for Determination HP-5ms, 30 m x 0.32 mm id, 0.25 m film of PCBs in Waste Materials by GC DB-XLB, 30 m x 0.25 mm id, 0.25 m film (Agilent preferred)
GC and GC/MS
D 6352
Standard Test Method for Boiling Range DB-HT Sim Dis, 5 m x 0.53 mm id, 0.15 m film Distribution of Petroleum Distillates in Boiling Range from 174 to 700 by GC Standard Test Method for Estimation of Engine Oil Volatility by Capillary GC DB-HT Sim Dis, 5 m x 0.53 mm id, 0.15 m film
D 6417 D2360 E 1616 E 1863 E 202 E 475
145-1001 19091N-116 19091Z-115 123-7364 125-1334 19095J-623
Standard Test Method for Trace Impurities HP-INNOWax, 60 m x 0.32 mm id, 0.25 m film in Monocylic Aromatic Hydrocarbons by GC Standard Test for Analysis of Acetic Anhydride Using GC Standard Test Method for Analysis of Acrylonitrile by GC Standard Test Methods for Analysis of Ethylene Glycols and Propylene Glycols Standard Test Method for Assay of Di-tert-Butyl Peroxide Using GC HP-1, 50 m x 0.32 mm id, 0.52 m film DB-WAXetr, 60 m x 0.32 mm id, 1.0 m film DB-624, 30 m x 0.53 mm id, 3.0 m film (Agilent preferred) HP-5, 30 m x 0.53 mm id, 5.0 m film
322
GC Columns
USP GC Phases
USP GC Phases
United States Pharmacopoeia (USP GC Phases) USP G1 G2 G3 G5 G6 G7 G14 G15 G16 G17 G19 G20 G25 G27 G28 G32 G35 G36 G38 G39 G41 G42 G43 G45 G46 Phase Composition Dimethylpolysiloxane oil Dimethylpolysiloxane gum 50% Phenyl - 50% methylpolysiloxane 3-cyanopropyl polysiloxane Trifluoropropylmethylpolysilicone 50% 3-Cyanopropyl - 50% phenylmethylsilicone Polyethylene glycol (average molecular weight of 950-1,050) Polyethylene glycol (average molecular weight of 3,000-3,700) Polyethylene glycol (average molecular weight of 15,000) 75% Phenyl - 25% methylpolysiloxane 25% Phenyl - 25% cyanopropylmethylsilicone Polyethylene glycol (average molecular weight of 380-420) Polyethylene glycol TPA (Carbowax 20M terephthalic acid) 5% Phenyl - 95% methylpolysiloxane 25% Phenyl - 75% methylpolysiloxane 20% Phenylmethyl - 80% dimethylpolysiloxane 1% Vinyl - 5% phenylmethylpolysiloxane Phase G1 plus a tailing inhibitor Polyethylene glycol (average molecular weight of 1,500) Phenylmethyldimethylsilicone (10% phenyl substituted) 35% Phenyl - 65% dimethylvinylsiloxane 6% Cyanopropylphenyl - 94% dimethylpolysiloxane Divinylbenzene-ethylene glycol-dimethacrylate 14% Cyanopropylphenyl - 86% methylpolysiloxane Agilent Phase Recommendation HP-1*, DB-1*, HP-1ms*, DB-1ms* HP-1*, DB-1*, HP-1ms*, DB-1ms* DB-17*, HP-50+* DB-23 DB-200, DB-210 DB-225, DB-225ms DB-WAX DB-WAX DB-WAX* DB-17, HP-50+ DB-225*, DB-225ms DB-WAX DB-FFAP*, HP-FFAP* DB-5*, HP-5*, HP-5ms*, DB-5ms DB-35, HP-35, DB-35ms DB-35, HP-35, DB-35ms DB-5, HP-5, HP-5ms, DB-5ms DB-1, HP-1, HP-1ms, DB-1ms DB-WAX DB-5, HP-5, HP-5ms, DB-5ms DB-35*, HP-35*, DB-35ms DB-624*, DB-1301 HP-PLOT U* DB-1701* *Indicates an exact equivalent
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Polyethylene glycol & diepoxide esterified with nitroterepthalic acid DB-FFAP*, HP-FFAP*
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Troubleshooting/Maintenance
Column Installation and Tools

Capillary Column Installation Quick Reference Guide
For more detailed installation information, refer to the GC Column Installation Guide which is provided with each column.
Precolumn Installation Check List

1. 2. Replace oxygen, moisture, and hydrocarbon traps as needed. Check gas cylinder pressures to ensure that an adequate supply of carrier, makeup, and fuel gases is available. Minimum recommended carrier gas purity percentages are: Helium 99.995 and Hydrogen 99.995. Clean the injection port, replace critical injection port seals, replace injection port liners, and change septa as needed. Check detector seals, and replace as necessary. Clean or replace detector jets as necessary. Carefully inspect the column for damage or breakage. Gather the necessary installation tools: You will need a column cutter, column nuts, ferrules, a magnifying loupe, and typewriter correction fluid.
3. 4. 5. 6.
Installing the Column

1. 2. 3.
0.4 0.4 0.4 0.4 0.5 0.8 0.8
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Table 1. Ferrule Sizes

Column ID 0.10 0.18 0.20 0.25 0.32 0.45 0.53 Ferrule ID (mm)
4. 5.
6. 7.
8.
Uncoil approximately 0.5 m of tubing from the column basket at both ends of the column for injector and detector installation. Avoid sharp bends in the tubing. Mount the column in the oven. Use the hanging bracket if available. Install the column nut and Vespel or graphite ferrule at each column end; pull the nut and ferrule down the tubing approximately 5 cm. (Table 1) Score (cut) the column. Use a light touch to score the column about 4 to 5 cm from each end. Make a clean break. Grasp the column between the thumb and forefinger as close to the score point as possible. Gently PULL and bend the column. The column should part easily. If the column doesn't break easily, don't force it! Score the column again in a different place, and try for a clean break. Use a magnifying loupe to inspect the cut. Make sure the cut is square across the tubing with no polyimide or glass fragments at the end of the tube. Install the column in the inlet. Check the GC manufacturer's instrument manual for the correct insertion distance. Mark the correct distance on the column with typewriter correction fluid. Insert the column into the injector. Finger tighten the column nut until it starts to grab the column, and then tighten the nut an additional 1/4 to 1/2 turn so that the column cannot be pulled from the fitting when gentle pressure is applied. Turn on the carrier gas and establish the proper flow rate. Set head pressure, split flow, and septum purge flow to appropriate levels. See Table 2 on page 325 for nominal head pressures. If using a split/splitless inlet, check that the purge (split) valve is ON (open).
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Installing the Column (continued)
9. Confirm carrier gas flow through the column. Immerse the end of the column in a vial of solvent, and check for bubbles. 10. Install the column into the detector. Check the instrument manufacturer's manual for the proper insertion distance. 11. Check for leaks. THIS IS VERY IMPORTANT. Don't even THINK about heating the column without thoroughly checking for leaks. 12. Establish proper injector and detector temperatures. 13. Establish proper makeup and detector gas flows. Ignite or turn ON the detector. 14. Purge the column for a minimum of 10 minutes at ambient temperature. Add the appropriate additional purge time following inlet or trap maintenance. 15. Inject a nonretained substance to check for proper injector installation. Examples: butane or methane (FID), headspace vapors from acetonitrile (NPD), headspace vapors from methylene chloride (ECD), air (TCD), argon (mass spectrometer). Proper installation is indicated by a symmetrical peak. If tailing is observed, repeat the injector installation process.
Conditioning and Testing the Column

1. Condition the column at 20C above the maximum temperature of the analysis or at the maximum temperature of the column (whichever is lower) for 2 hours. If after 10 minutes at the upper temperature the background does not begin to fall, immediately cool the column and check for leaks. 2. If you are using Vespel or graphite/Vespel ferrules, recheck tightness after the conditioning process. 3. Confirm final proper average linear velocity by injecting a non-retained substance.
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Table 2. Approximate Head Pressures (psig)

Column Length (m) 10 12 15 20 25 30 40 50 60 75 105 20-40 40-60 30-45 20-30 6-10 8-14 4-8 5-10 7-15 10-20 20-30 15-25 10-20 3-5 2-4 0.18 5-10 10-15 8-12 5-10 1-2 0.2 Column ID (mm) 0.25 0.32 0.45 0.53
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Troubleshooting/Maintenance Capillary GC Columns
On the most basic level, a capillary column is composed of two parts - the tubing and stationary phase (Figure 1). Fused silica (with an external polyimide coating) and stainless steel are the primary tubing materials. There are numerous stationary phases. Most are high molecular weight, thermally stable polymers that are liquids or gums. The most common stationary phases of this type are the polysiloxanes (sometimes incorrectly called silicones) and polyethylene glycols. The next most common type of stationary phases are small, porous particles composed of polymers or zeolites (e.g., alumina, molecular sieves).
Figure 1. GC Capillary Column
Stationary Phase Selection

1. If no information or ideas about which stationary phase to use is available, start with a DB-1 or DB-5. 2. Low bleed (ms) columns are usually more inert and have higher temperature limits. 3. Use the least polar stationary phase that provides satisfactory resolution and analysis times. Non-polar stationary phases have superior lifetimes to polar phases. 4. Use a stationary phase with a polarity similar to that of the solutes. This approach works more times than not; however, the best stationary phase is not always found using this technique. 5. If poorly separated solutes possess different dipoles or hydrogen bonding strengths, change to a stationary phase with a different amount (not necessarily more) of the dipole or hydrogen bonding interaction. Other co-elutions may occur upon changing the stationary phase, thus the new stationary phase may not provide better overall resolution. 6. If possible, avoid using a stationary phase that contains a functionality that generates a large response with a selective detector. For example, cyanopropyl containing stationary phases exhibit a disproportionately large baseline rise (due to column bleed) with NPDs. 7. A DB-1 or DB-5, DB-1701, DB-17 and DB-WAX cover the widest range of selectivities with the smallest number of columns. 8. PLOT columns are used for the analysis of gaseous samples at above ambient column temperatures.
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Troubleshooting/Maintenance Column Diameter Selection
1. Use 0.18-0.25 mm id columns when higher column efficiencies are needed. 0.18 mm id columns are especially well suited for GC/MS systems with low pumping capacities. Smaller diameter columns have the lowest capacities and require the highest head pressures. 2. Use 0.32 mm id columns when higher sample capacity is needed. They often provide better resolution of earlier eluting solutes for splitless injections or large injection volumes (>2 L) than 0.25 mm id columns. 3. Use 0.45 mm id columns when only a Megabore direct injector is available and higher column efficiency is desired. Well suited for high carrier gas flow rate situations such as with purge & trap and headspace samplers. 4. Use 0.53 mm id columns when only a Megabore direct injector is available. Well suited for high carrier gas flow rate situations such as with purge & trap and head-space samplers. 0.53 mm id columns have the highest sample capacities at constant df.
Column Length Selection

1. Start with 25-30 meter columns when the best length is unknown. 2. 10-15 meter columns are well suited for samples containing very well separated solutes or very few solutes. Shorter lengths are used for very small diameter columns to reduce head pressures. 3. 50-60 meters should be used when resolution is not possible by other means (smaller diameter, different stationary phase, change in column temperature). Best suited for complex samples containing a large number of solutes. Long columns have long analysis times and higher cost.
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Column Film Thickness Selection

1. For 0.18-0.32 mm id columns, a film thickness of 0.18-0.25 m is average or standard (i.e., not thin or thick) and used for most analyses. 2. For 0.45-0.53 mm id columns, a film thickness of 0.8-1.5 m is average or standard (i.e., not thin or thick) and used for most analyses. 3. Thick film columns are used to retain and resolve volatile solutes (e.g., light solvents, gases). Thick columns are more inert and have higher capacities. Thick film columns exhibit higher column bleed and decreased upper temperature limits. 4. Thin film columns are used to minimize the retention of high boiling, high molecular weight solutes (e.g., steroids, triglycerides). Thin columns are less inert and have lower capacities. Thin film columns exhibit lower column bleed.
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Equation 1. Average Linear Velocity
u (cm/sec) = L tM
Carrier Gases
The carrier gas linear velocity or flow rate directly influences retention time and efficiency. The proper selection and setting of the carrier gas are essential to obtaining the best analysis times, efficiency and reproducibility. The carrier gas linear velocity or flow rate is controlled by adjusting the carrier gas pressure at the front of the column (commonly called the head pressure). The pressure setting is dependent on the type of carrier gas, the column length and diameter, column temperature, and the desired linear velocity or flow rate. For capillary columns, the average linear velocity ( ) is a better and more meaningful measure of the carrier gas than the flow rate (F). The average linear velocity can be thought of as the average speed of the carrier gas in cm/sec (i.e., centimeters of column traveled per second by a carrier gas molecule). The average linear velocity is calculated using Equation 1. Carrier gas linear velocities (and flow rates) are dependent on column temperature. At a constant head pressure, carrier gas linear velocities decrease as column temperature increases. This means the average linear velocity needs to be set at the same temperature for a given method if reproducible results are desired. Setting the average linear velocity at a different column temperature results in retention and resolution changes. Since the average linear velocity is dependent on column temperature, the velocity decreases during a temperature program run. Electronic pressure controlled injectors can be programmed to maintain a constant average linear velocity or flow rate throughout the temperature program. Using this feature may result in better resolution of the later eluting peaks or shorter analysis times. Contrary to common perception, hydrogen is a safe carrier gas. It is extremely diffusive in air and only explosive when air is present within a narrow concentration range. In other words, it is difficult to create a hydrogen explosion under GC conditions. Finally, safety features built into modern GCs greatly reduce any explosion possibilities and hazards.
L = tM =
column length (cm) retention time of an unretained peak (sec)
Recommended average linear velocities

He: 30-40 cm/sec H2: 50-80 cm/sec
Unretained Compounds
Detector Unretained Compound
FID ECD methane, butane1 methylene chloride2,3, dichlorodifluoromethane NPD acetonitrile4 TCD, MS methane, butane1, air PID, ELCD vinyl chloride 1. 2. From a disposable lighter. Place 1-2 drops in an autosampler vial and tightly cap. Shake and inject 1-2 L from the headspace of the vial. Do not inject any liquid. Use a column temperature above 50C. Use a column temperature above 90C.
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3. 4.
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Troubleshooting/Maintenance Guard Columns or Retention Gap
A guard column and retention gap are the same thing, but they serve different purposes. Both are 1-10 meters of deactivated fused silica tubing attached to the front of the column. Deactivated fused silica tubing does not contain any stationary phase; however, the surface is deactivated to minimize solute interactions. A suitable union is used to attach the tubing to the column. In most cases, the diameter of the retention gap or guard column should be the same as the column. If the tubing sizes are different, it is better to have a larger diameter guard column or retention gap than a smaller one. Guard columns are used when samples contain nonvolatile residues that may contaminate a column. The nonvolatile residues deposit in the guard column and not in the column. This greatly reduces the interaction between the residues and the sample since the guard column does not retain the solutes (because it contains no stationary phase). Also, the residues do not coat the stationary phase which often results in poor peak shapes. Periodic cutting or trimming of the guard column is usually required upon a build-up of residues. Guard columns are often 5-10 meters in length to allow substantial trimming before the entire guard column has to replaced. The onset of peak shape problems is the usual indicator that the guard column needs trimming or changing. Retention gaps are used to improve peak shapes for some types of samples, columns, and GC conditions. Usually a minimum of 3-5 meters of tubing is required to obtain the benefits of a retention gap. The situations that benefit the most from retention gaps are large volume injections (>2 L) and solvent-stationary phase polarity mismatches for splitless, Megabore direct and on-column injections. Peak shapes are sometimes distorted when using combinations of these conditions. Polarity mismatches occur when the sample solvent and column stationary phase are very different in polarity. The greatest improvement is seen for the peaks eluting closest to the solvent front or solutes very similar to the solvent in polarity. The benefits of a retention gap are often unintentionally obtained when using a guard column.
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Detector Injector
Deactivated Fused Silica Tubing
Union Column
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Causes of Column Performance Degradation

Column Breakage
Fused silica columns break wherever there is a weak point in the polyimide coating. The polyimide coating protects the fragile fused silica tubing. The continuous heating and cooling of the oven, vibrations caused by the oven fan, and being wound on a circular cage all place stress on the tubing. Eventually breakage occurs at a weak point. Weak spots are created when the polyimide coating is scratched or abraded. This usually occurs when a sharp point or edge is dragged over the tubing. Column hangers and tags, metal edges in the GC oven, column cutters, and miscellaneous items on the lab bench are just some of the common sources of sharp edges or points. It is rare for a column to spontaneously break. Column manufacturing practices tend to expose any weak tubing and eliminate it from use in finished columns. Larger diameter columns are more prone to breakage. This means that greater care and prevention against breakage must be taken with 0.45-0.53 mm id tubing than with 0.18-0.32 mm id tubing. A broken column is not always fatal. If a broken column was maintained at a high temperature either continuously or with multiple temperature program runs, damage to the column is very likely. The back half of the broken column has been exposed to oxygen at elevated temperatures which rapidly damages the stationary phase (see Thermal and Oxygen Damage on pages 330331). The front half is fine since carrier gas flowed through this length of column. If a broken column has not been heated or only exposed to high temperatures or oxygen for a very short time, the back half has probably not suffered any significant damage. A union can be installed to repair a broken column. Any suitable union will work to rejoin the column (see pages 164166). No more than 2-3 unions should be installed on any one column. Problems with dead volume (peak tailing) may occur with multiple unions.
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Thermal Damage
Exceeding a columns upper temperature limit results in accelerated degradation of the stationary phase and tubing surface. This results in the premature onset of excessive column bleed, peak tailing for active compounds and/or loss of efficiency (resolution). Fortunately, thermal damage is a slower process, thus prolonged times above the temperature limit are required before significant damage occurs. Thermal damage is greatly accelerated in the presence of oxygen. Overheating a column with a leak or high oxygen levels in the carrier gas results in rapid and permanent column damage. Setting the GCs maximum oven temperature at or a few degrees above the columns temperature limit is the best method to prevent thermal damage. This prevents the accidental overheating of the column. If a column is thermally damaged, it may still be functional. Remove the column from the detector. Heat the column for 8-16 hours at its isothermal temperature limit. Remove 10-15 cm from the detector end of the column. Reinstall the column and condition as usual. The column usually does not return to its original performance; however, it is often still functional. The life of the column will be reduced after thermal damage.
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Troubleshooting/Maintenance Oxygen Damage
Oxygen is an enemy to most capillary GC columns. While no column damage occurs at or near ambient temperatures, severe damage occurs as the column temperature increases. In general, the temperature and oxygen concentration at which significant damage occurs is lower for polar stationary phases. It is constant exposure to oxygen that is the problem. Momentary exposure such as an injection of air or a very short duration septum nut removal is not a problem. A leak in the carrier gas flow path (e.g., gas lines, fittings, injector) is the most common source of oxygen exposure. As the column is heated, very rapid degradation of the stationary phase occurs. This results in the premature onset of excessive column bleed, peak tailing for active compounds and/or loss of efficiency (resolution). These are the same symptoms as for thermal damage. Unfortunately, by the time oxygen damage is discovered, significant column damage has already occurred. In less severe cases, the column may still be functional but at a reduced performance level. In more severe cases, the column is irreversibly damaged. Maintaining an oxygen and leak free system is the best prevention against oxygen damage. Good GC system maintenance includes periodic leak checks of the gas lines and regulators, regular septa changes, using high quality carrier gases, installing and changing oxygen traps, and changing gas cylinders before they are completely empty.
Chemical Damage
There are relatively few compounds that damage stationary phases. Introducing nonvolatile compounds (high molecular weight or high boiling point) in a column often degrades performance, but damage to the stationary phase does not occur. These residues can often be removed and performance returned by solvent rinsing the column (see next page for more information on column contamination and solvent rinsing). Inorganic or mineral bases and acids are the primary compounds to avoid introducing in a column. The acids include hydrochloric (HCl), sulfuric (H2SO4), nitric (HNO3), phosphoric (H3PO4) and chromic (CrO3). The bases include potassium hydroxide (KOH), sodium hydroxide (NaOH) and ammonium hydroxide (NH4OH). Most of these acids and bases are not very volatile and accumulate at the front of the column. If allowed to remain, the acids or bases damage the stationary phase. This results in the premature onset of excessive column bleed, peak tailing for active compounds and/or loss of efficiency (resolution). The symptoms are very similar to thermal and oxygen damage. Hydrochloric acid and ammonium hydroxide are the least harmful of the group. Both tend to follow any water that is present in the sample. If the water is not or only poorly retained by the column, the residence time of HCl and NH4OH in the column is short. This tends to eliminate or minimize any damage by these compounds. Thus, if HCl or NH4OH are present in a sample, using conditions or a column with no water retention will render these compounds relatively harmless to the column.
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The only organic compounds that have been reported to damage stationary phases are perfluoroacids. Examples include trifluoroacetic, pentafluoropropanoic and heptafluorobutyric acid. They need to be present at high levels (e.g., 1% or higher). Most of the problems are experienced with splitless or Megabore direct injections where large volumes of the sample are deposited at the front of the column. Since chemical damage is usually limited to the front of the column, trimming or cutting 1/2-1 meter from the front of the column often eliminates any chromatographic problems. In more severe cases, 5 or more meters may need to be removed. The use of a guard column or retention gap will minimize the amount of column damage; however, frequent trimming of the guard column may be necessary. The acid or base often damages the surface of the deactivated fused silica tubing which leads to peak shape problems for active compounds.
Column Contamination
Column contamination is one of the most common problems encountered in capillary GC. Unfortunately, it mimics a very wide variety of problems and is often misdiagnosed as another problem. A contaminated column is usually not damaged, but it may be rendered unusable. There are two basic types of contaminants: nonvolatile and semivolatile. Nonvolatile contaminants or residues do not elute and accumulate in the column. The column becomes coated with these residues which interfere with the proper partitioning of solutes in and out of the stationary phase. Also, the residues may interact with active solutes resulting in peak adsorption problems (evident as peak tailing or loss of peak size). Active solutes are those containing a hydroxyl (-OH) or amine (-NH) group, and some thiols (-SH) and aldehydes. Semivolatile contaminants or residues accumulate in the column, but eventually elute. Hours to days may elapse before they completely leave the column. Like nonvolatile residues, they may cause peak shape and size problems and, in addition, are usually responsible for many baseline problems (instability, wander, drift, ghost peaks, etc.). Contaminants originate from a number of sources, with injected samples being the most common. Extracted samples are among the worst types. Biological fluids and tissues, soils, waste and ground water, and similar types of matrices contain high amounts of semivolatile and nonvolatile materials. Even with careful and thorough extraction procedures, small amounts of these materials are present in the injected sample. Several to hundreds of injections may be necessary before the accumulated residues cause problems. Injection techniques such as on-column, splitless and Megabore direct place a large amount of sample into the column, thus column contamination is more common with these injection techniques. Occasionally, contaminants originate from materials in gas lines and traps, ferrule and septa particles, or anything coming in contact with the sample (vials, solvents, syringes, pipettes, etc.). These types of contaminants are probably responsible when a contamination problem suddenly develops and similar samples in previous months or years did not cause any problems.
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Minimizing the amount of semivolatile and nonvolatile sample residues is the best method to reduce contamination problems. Unfortunately, the presence and identity of potential contaminants are often unknown. Rigorous and thorough sample cleanup is the best protection against contamination problems. The use of a guard column or retention gap (see page 329) often reduces the severity or delays the onset of column contamination induced problems. If a column becomes contaminated, it is best to solvent rinse the column to remove the contaminants. Maintaining a contaminated column at high temperatures for long periods of time (often called baking out a column) is not recommended. Baking out a column may convert some of the contaminating residues into insoluble materials that cannot be solvent rinsed from the column. If this occurs, the column cannot be salvaged in most cases. Sometimes the column can be cut in half and the back half may still be useable. Baking out a column should be limited to 1-2 hours at the isothermal temperature limit of the column.
Solvent Rinsing Columns

Solvent rinsing columns involves removing the column from the GC and passing milliliters of solvent through the column. Any residues soluble in the rinse solvents are washed from the column. Injecting large volumes of solvent while the column is still installed is not rinsing a column nor does it remove any contaminants from the column. A capillary GC column must have a bonded and cross-linked stationary phase before it can be solvent rinsed. Solvent rinsing a nonbonded stationary phase results in severe damage to the column. A column rinse kit is used to force solvent through the column (Figure 2). The rinse kit is attached to a pressurized gas source (N2 or He), and the column is inserted into the rinse kit. Solvent is added to the vial, and the vial is pressurized using the gas source. The pressure forces solvent to flow through the column. Residues dissolve into the solvent and are backflushed out of the column with the solvent. The solvent is then purged from the column, and the column is properly conditioned. Before rinsing a column, cut about 1/2 meter from the front (i.e., injector end) of the column. Insert the detector end of the column into the rinse kit. Multiple solvents are normally used to rinse columns. Each successive solvent must be miscible with the previous one. High boiling point solvents should be avoided especially as the last solvent. The sample solvent(s) is often a good choice. Methanol, methylene chloride and hexane are recommended and work very well for the majority of cases. Acetone can be substituted for methylene chloride to avoid using halogenated solvents; however, methylene chloride is one of the best rinsing solvents. If aqueous based samples (e.g., biological fluids and tissues) were injected, use water before the methanol. Some residues originating from aqueous based samples are only soluble in water and not organic solvents. Water and alcohols (e.g., methanol, ethanol, isopropanol) should be used to rinse bonded polyethylene glycol based stationary phases (e.g., DB-WAX, DBWAXetr, DB-FFAP, HP-Innowax) only as a last resort.
Table 3. Solvent Volumes for Rinsing Columns

Column ID (mm) 0.18-0.2 0.25 0.32 0.45 0.53 Solvent Volume (mL) 3-4 4-5 6-7
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7-8 10-12
Using larger volumes will not damage the column.
Figure 2. Solvent Rinse Kit
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Table 3 (page 333) lists the suggested solvent volumes for different diameter columns. Using larger sovent volumes is not harmful, but rarely better and merely wasteful. After adding the first solvent, pressurize the rinse kit, but stay below 20 psi. Use the highest pressure that keeps the solvent flow rate below 1 mL/min. Except for most 0.53 mm id columns, the rinse kit pressure will reach 20 psi before the flow rate reaches 1 mL/min. Longer rinse times are required when using heavy or viscous solvents, and for longer or smaller diameter columns. When all or most of the first solvent has entered the column, add the next solvent. The previous solvent does not have to vacate the column before the next solvent is started through the column. After the last solvent has left the column, allow the pressurizing gas to flow through the column for 5-10 minutes. Install the column in the injector and turn on the carrier gas. Allow the carrier gas to flow through the column for 5-10 minutes. Attach the column to the detector (or leave it unattached if preferred). Using a temperature program starting at 40-50C, heat the column at 2-3/min until the upper temperature limit of the column is reached. Maintain this temperature for 1-4 hours until the column is fully conditioned.
Column Storage
Capillary columns should be stored in their original box when removed from the GC. Place the GC septa over the ends to prevent debris from entering the tubing. Upon reinstallation of the column, the column ends need to be trimmed by 2-4 cm to ensure that a small piece of septa is not lodged in the column. If a column is left in a heated GC, there should always be carrier gas flow. The carrier gas flow can be turned off only if the oven, injector, detector and transfer lines are turned off (i.e., not heated). Without carrier gas flow, damage to the heated portion of the column occurs.
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Evaluating the Problem

The first step in any troubleshooting effort is to step back and evaluate the situation. Rushing to solve the problem often results in a critical piece of important information being overlooked or neglected. In addition to the problem, look for any other changes or differences in the chromatogram. Many problems are accompanied by other symptoms. Retention time shifts, altered baseline noise or drift, or peak shape changes are only a few of the other clues that often point to or narrow the list of possible causes. Finally, make note of any changes or differences involving the sample. Solvents, vials, pipettes, storage conditions, sample age, extraction, preparation techniques, or any other factor influencing the sample environment can be responsible.
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Troubleshooting/Maintenance Checking the Obvious
A surprising number of problems involve fairly simple and often overlooked components of the GC system or analysis. Many of these items are transparent in the daily operation of the GC and are often taken for granted (set it and forget it). The areas and items to check include: 1. Gases - pressures, carrier gas average linear velocity, and flow rates (detector, split vent, septum purge). 2. Temperatures - column, injector, detector and transfer lines. 3. System parameters - purge activation times, detector attenuation and range, mass ranges, etc. 4. Gas lines and traps - cleanliness, leaks, and expiration. 5. Injector consumables - septa, liners, O-rings and ferrules. 6. Sample integrity - concentration, degradation, solvent, and storage. 7. Syringes - handling technique, leaks, needle sharpness, and cleanliness. 8. Data system - settings and connections.
The Most Common Problems

Ghost Peaks or Carryover
System contamination is responsible for most ghost peaks or carryover problems. If the extra ghost peaks are similar in width to the sample peaks (with similar retention times), the contaminants were most likely introduced into the column at the same time as the sample. The extra compounds may be present in the injector (i.e., contamination) or in the sample itself. Impurities in solvents, vials, caps and syringes are only some of the possible sources. Injecting sample and solvent blanks may help to find possible sources of the contaminants. If the ghost peaks are much broader than the sample peaks, the contaminants were most likely already in the column when the injection was made. These compounds were still in the column when a previous GC run was terminated. They elute during a later run and are often very broad. Sometimes numerous ghost peaks from multiple injections overlap and elute as a hump or blob. This often takes on the appearance of baseline drift or wander. Increasing the final temperature or time in the temperature program is one method to minimize or eliminate a ghost peak problem. Alternatively, a short bake-out after each run or series of runs may remove the highly retained compounds from the column before they cause a problem. Performing a condensation test is a good method to determine whether a contaminated injector is the source of the carryover or ghost peaks.
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Condensation Test
Use this test whenever injector or carrier gas contamination problems are suspected (e.g., ghost peaks or erratic baselines). 1. Leave the GC at 40-50C for 8 or more hours. 2. Run a blank analysis (i.e., start the GC, but with no injection) using the normal temperature conditions and instrument settings. 3. Collect the chromatogram for this blank run. 4. Immediately repeat the blank run as soon as the first one is completed. Do not allow more than 5 minutes to elapse before starting the second blank run. 5. Collect the chromatogram for the second blank run and compare it to the first chromatogram. 6. If the second chromatogram contains a substantially larger amount of peaks and baseline instability, the incoming carrier gas line or the carrier gas is contaminated. 7. If the second chromatogram contains few peaks or very little baseline drift, the carrier gas and incoming carrier gas lines are relatively clean.
Excessive Baseline Noise

Possible Cause Injector contamination Column contamination Solution Bake-out the column Solvent rinse the column Comments Limit the bake-out to 1-2 hours Only for bonded and cross-linked phases Check for inlet contamination Detector contamination Contaminated or low quality gases Column inserted too far into the detector Incorrect detector gas flow rates Leak when using an MS, ECD or TCD Old detector filament, lamp or electron multiplier Septum degradation Clean the detector Use better grade gases; also check for expired gas traps or leaks Reinstall the column Adjust the flow rates to the recommended values Find and eliminate the leak Replace appropriate part Replace septum For high temperature applications use an appropriate septum Usually the noise increases over time and not suddenly Usually occurs after changing a gas cylinder Consult GC manual for the proper insertion distance Consult the GC manual for the proper flow rates Usually at the column fittings or injector Clean the injector, replace liner, gold seal Try a condensation test; gas lines may also need cleaning
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Baseline Instability or Disturbances
Possible Cause Injector contamination Column contamination Unequilibrated detector Incompletely conditioned column Change in carrier gas flow rate during the temperature program Solution Clean the injector Bake-out the column Allow the detector to stabilize Fully condition the column Normal in many cases Comments Try a condensation test; gas lines may also need cleaning Limit a bake-out to 1-2 hours Some detectors may require up to 24 hours to fully stabilize More critical for trace level analyses MS, TCD and ECD respond to changes in carrier gas flow rate
Baseline Instability or Disturbances

Possible Cause Column contamination Solution Trim the column Solvent rinse the column Column activity Solvent-phase polarity mismatch Irreversible. Replace the column Change sample solvent Install a retention gap Solvent effect violation for splitless or on-column injections Too low of a split ratio Poor column installation Some active compounds always tail Decrease the initial column temperature Increase the split ratio Reinstall the column None Comments Remove 1/2-1 meter from the front of the column Only for bonded and cross-linked phases Check the inlet for contamination Only affects active compounds More tailing for the early eluting peaks or those closest to the solvent front 3-5 meter retention gap is sufficient Peak tailing decreases with retention
GC and GC/MS
Flow from split vent should be 20 mL/min or higher More tailing for the early eluting peaks Most common for amines and carboxylic acids
337
GC and GC/MS
Split Peaks
Possible Cause Injection technique Mixed sample solvent to a single solvent Poor column installation Sample degradation in the injector Solution Change technique Change the sample solvent Reinstall the column in the injector Reduce the injector temperature Change to an on-column injection Poor sample focusing Use a retention gap Comments Usually related to erratic plunger depression or having sample in the syringe needle. Use an auto injector. Worse for solvents with large differences in polarity or boiling points Usually a large error in the insertion distance Peak broadening or tailing may occur if the temperature is too low. Requires an on-column injector For splitless and on-column injection
Retention Time Shift

Possible Cause Change in carrier gas velocity Change in column temperature Change in column dimension Large change in compound concentration Solution Check the carrier gas velocity Check the column temperature Verify column identity Try a different sample concentration Leak check the injector Clean or replace the plugged line Replace the septum Change solvent, use a retention gap May also affect adjacent peaks. Sample overloading is corrected with an increase split ratio or sample dilution A change in peak size usually occurs also More common for the split line; also check flow controllers and solenoids Check for needle barb For splitless injection Comments All peaks will shift in the same direction by approximately the same amount Not all peaks will shift by the same amount
GC and GC/MS
Leak in the injector Blockage in a gas line Septum leak Sample solvent incompatibility
338
GC and GC/MS
Change in Peak Size
Possible Cause Change in detector response Solution Check gas flows, temperatures and settings Check background level or noise Change in the split ratio Change in the purge activation time Change in injection volume Change in sample concentration Leak in the syringe Column contamination Column activity Coelution Change in injector discrimination Sample flashback Decomposition from inlet contamination Check split ratio Check the purge activation time Check the injection technique Check and verify sample concentration Use a different syringe Trim the column Solvent rinse the column Irreversible Change column temperature or stationary phase Maintain the same injector parameters Inject less, use a larger liner, reduce the inlet temperature Clean the inlet, replace the liner, replace the gold seal Comments All peaks may not be equally affected May be caused by system contamination and not the detector All peaks will not be equally affected For splitless injectors Injection volumes are not linear Changes may also be caused by degradation, evaporation, or variances in sample temperature or pH Sample leaks passed the plunger or around the needle; leaks are often not readily visible Remove 1/2-1 meter from the front of the column Only for bonded and cross-linked phases Only affects active compounds Decrease column temperature and check for the appearance of a peak shoulder or tail Most severe for split injections
GC and GC/MS
Less solvent and higher flow rates are most helpful Only use deactivated liners and glass wool in the inlet
339
GC and GC/MS
Loss of Resolution
Possible Cause Decrease in separation Different column temperature Different column dimensions or phase Coelution with another peak Increase in peak width Change in carrier gas velocity Column contamination Change in the injector Change in sample concentration or solvent Improper solvent effect, lack of focusing Solution Check column temperature Verify column identity Change the column temperature Check carrier gas velocity Trim the column Solvent rinse the column Check the injector settings Try a different sample concentration Lower oven temperature, better solvent, sample phase polarity match, use a retention gap Comments Differences in other peaks will be visible Differences in other peaks will be visible Decrease column temperature and check for the appearance of a peak shoulder or tail A change in retention time also occurs Remove 1/2-1 meter from the front of the column Only for bonded and cross-linked phases Typical areas: split ratio, liner, temperature, injection volume Peak widths increase at higher concentrations For splitless injectors
GC and GC/MS
340
GC and GC/MS Applications

Solvent Retention Data

DB-624 - Alphabetical Order
Column: P/N: Oven: DB-624 30 m x 0.53 mm id, 3 m 125-1334 40C for 5 min 40-260C at 10/min 260C for 3 min Helium, constant pressure at 30 cm/sec (40C) Split 1:10, 250C FID, 300C
Retention Time 20.02 13.43 10.37 18.69 17.96 18.79 16.46 6.11 9.62 4.00 7.16 5.33 8.72 10.17 12.86 7.16 8.34 11.38 12.30 18.24 21.46 18.04 16.78 9.92 20.35 20.73 12.94 17.06 10.38 7.30 13.73 15.58 20.58 11.22 3.47 10.98 15.57 7.34 10.02 14.42 21.22 12.15 15.84 10.95 8.64 3.72 4.27 18.40 22.65 23.03 10.37 3.72 9.18 3.24 3.72 14.63 15.32 11.93 8.65 15.60 9.15 22.97 17.22 15.63 15.53 15.46 15.29 14.95 17.22 17.22 21.69 26.63 13.14 5.82 21.30 15.06 13.31 13.16 13.01 12.80 15.19 14.88 14.24 14.98 18.10 4.27
Compound 1,2-dibromo-3-chloropropane (DBCP) 1,2-dibromoethane (EDB) dibromomethane 1,2-dichlorobenzene 1,3-dichlorobenzene 1,4-dichlorobenzene trans-1,4-dichloro-2-butene 1,1-dichloroethane 1,2-dichloroethane (ethylene dichloride) 1,1-dichloroethylene (vinylidine chloride) cis-1,2-dichloroethylene trans-1,2-dichloroethylene 1,1-dichloropropane 1,2-dichloropropane 1,3-dichloropropane 2,2-dichloropropane 1,1-dichloropropene cis-1,3-dichloropropene trans-1,3-dichloropropene diethylene glycol diethylene glycol monobutyl ether diethylene glycol monoethyl ether diethylene glycol monomethyl ether diglyme (diethylene glycol dimethyl ether) 1,3-diisopropylbenzene 1,4-diisopropylbenzene 2,4-dimethyl-3-pentanone (diisopropyl ketone) 2,6-dimethyl-4-heptanone (diisobutyl ketone) 1,4-dioxane 1,3-dioxolane DMF (dimethylformamide) DMSO (methyl sulfoxide) dodecane epichlorohydrin ethanol 2-ethoxyethanol (cellosolve) 2-ethoxyethyl acetate ethyl acetate ethyl acrylate ethylbenzene ethyl benzoate ethylene glycol ethylene glycol monobutyl ether ethylene glycol monoethyl ether ethylene glycol monomethyl ether ethyl ether ethyl formate 2-ethyl-1-hexanol -ethylphenethyl alcohol -ethylphenethyl alcohol ethyl propionate ethyl vinyl ether fluorobenzene fluorotrichloromethane (Freon 11) furan furfural furfuryl alcohol glycidol glyme (propylene glycol dimethyl ether) heptanal heptane 1,7-heptanediol 1-heptanol 2-heptanol 3-heptanol 2-heptanone 3-heptanone 4-heptanone trans-2-hepten-1-ol cis-4-hepten-1-ol hexachloro-1,3-butadiene hexadecane hexanal hexane 1,6-hexanediol 1-hexanol 2-hexanol 3-hexanol 2-hexanone 3-hexanone cis-2-hexen-1-ol cis-3-hexen-1-ol 4-hexen-3-one 4-hydroxy-4-methyl-2-pentanone iodobenzene iodomethane
Retention Time 14.75 8.60 12.19 17.68 5.66 8.81 21.04 4.27 8.87 15.88 6.23 6.01 7.53 2.59 8.67 4.60 19.90 11.87 8.73 11.78 9.21 7.91 12.85 14.82 4.80 2.80 16.52 14.73 11.85 11.64 12.28 13.20 19.60 7.88 19.66 17.22 17.68 5.30 12.98 20.35 21.60 22.40 22.79 19.71 14.63 26.02 20.97 17.76 12.11 24.54 19.17 17.63 17.45 27.10 25.26 10.25 3.37 19.52 10.34 12.63 10.60 10.60 10.11 10.34 10.20 12.63 10.03 6.93 18.53 22.65 22.91 13.13 6.34 6.21 3.91 11.89 7.25 10.51 16.56 22.92 13.16 9.05 7.66 13.07 7.55 11.70
Compound iso-amyl acetate iso-butanol iso-butyl acetate iso-butylbenzene iso-butyraldehyde iso-octane isophorone iso-propanol iso-propyl acetate iso-propylbenzene (cumene) iso-propyl ether methacrolein methacrylonitrile methanol 2-methoxyethanol (methyl cellosolve) methyl acetate methyl benzoate 2-methyl-1-butanol (active amyl alcohol) 2-methyl-2-butanol (tert-amyl alcohol) 3-methyl-1-butanol (iso-amyl alcohol) 3-methyl-2-butanone 2-methyl-3-buten-2-ol 3-methyl-2-buten-1-ol 2-methylbutyl acetate methylene chloride methyl formate 5-methyl-3-heptanone 5-methyl-2-hexanone 2-methyl-3-pentanone 4-methyl-2-pentanone(MIBK; methyl isobutyl ketone) 4-methyl-2-pentanol 4-methyl-3-penten-3-one -methylphenyl alcohol methyl propionate 1-methylpyrrolidone -methylstyrene 4-methylstyrene methyl tert-butyl ether (MTBE) morpholine nitrobenzene 2-nitrotoluene 3-nitrotoluene 4-nitrotoluene nonanal nonane 1,9-nonanediol 1-nonanol octanal octane 1,8-octanediol 1-octanol 2-octanone 3-octanone pentachlorobenzene pentadecane pentanal (valeraldehyde) pentane 1,5-pentanediol 2,3-pentanedione 1-pentanol 2-pentanol 3-pentanol 2-pentanone 3-pentanone 1-penten-3-ol 2-penten-1-ol 1-penten-3-one (ethyl vinyl ketone) 3-penten-2-one (methyl vinyl ketone) pentyl ether 2-phenoxyethanol 4-phenyl-2-butanone (benzyl acetone) 1,3-propanediol 1-propanol 2-propen-1-ol (allyl alcohol) propionaldehyde propionic acid propionitrile propyl acetate propylbenzene propyl benzoate propylene glycol (1,2-propanediol) propyl ether propyl formate propyl propionate 2-propyn-1-ol (propargyl alcohol) pyridine
Carrier: Injector: Detector:
Retention Time 10.58 2.46 9.10 4.05 4.27 19.69 3.81 12.21 5.22 9.65 6.41 15.57 17.45 8.69 18.21 21.07 19.27 29.08 16.39 7.59 10.64 4.27 15.61 16.10 17.70 14.14 6.99 9.73 7.19 9.87 9.02 15.89 13.24 18.69 14.41 9.34 7.10 6.84 4.27 8.34 14.25 8.25 13.25 7.75 14.21 23.06 4.57 19.35 16.71 16.82 16.82 9.18 8.10 15.63 16.04 13.16 13.39 16.82 27.43 22.65
Compound acetal (acetaldehyde diethyl acetal) acetaldeyde acetic acid acetone acetonitrile acetophenone acrolein acrylic acid acrylonitrile allyl ether allyl ethyl ether amyl acetate benzaldehyde benzene benzonitrile benzyl acetate benzyl alcohol benzyl ether bromobenzene bromochloromethane bromodichloromethane bromoethane bromoform 1,3-butanediol 1,4-butanediol 2,3-butanediol 2,3-butanedione (diacetal) 1-butanol 2-butanone (MEK) 2-buten-1-ol (crotyl alcohol) 3-buten-1-ol 2-butoxyethanol (butyl cellosolve) butyl acetate butylbenzene butyl ether butyl ethyl ether butyl methyl ether butyraldehyde carbon disulfide carbon tetrachloride chlorobenzene 1-chlorobutane chlorodibromomethane chloroform 1-chlorohexane 1-chloro-4-nitrobenzene 3-chloropropene (allyl chloride) 4-chlorostyrene 2-chlorotoluene 3-chlorotoluene 4-chlorotoluene crotonaldehyde cyclohexane cyclohexanol cyclohexanone cyclopentanol cyclopentanone decane 1,10-decanediol 1-decanol
GC and GC/MS
341

Retention Time 7.55 11.76 17.68 15.28 19.46 8.89 5.01 10.02 17.39 6.90 19.35 24.02 14.38 16.38 12.86 23.80 10.06 7.64 19.63 19.63 19.96 11.93 22.23 21.41 20.35 8.05 12.60 9.80 16.46 4.00 22.24 8.91 21.95 18.18 17.47 16.82 18.78 6.37 14.62 15.28 14.62 Compound sec-butanol sec-butyl acetate sec-butylbenzene styrene styrene oxide tert-amyl methyl ether tert-butanol tert-butyl acetate tert-butylbenzene tert-butyl ethyl ether 4-tert-butyltoluene 1,2,4,5-tetrachlorobenzene 1,1,1,2-tetrachloroethane 1,1,2,2-tetrachloroethane tetrachloroethylene tetradecane tetrahydropyran THF (tetrahydrofuran) m-tolualdehyde o-tolualdehyde p-tolualdehyde toluene 1,2,3-trichlorobenzene 1,2,4-trichlorobenzene 1,3,5-trichlorobenzene 1,1,1-trichloroethane 1,1,2-trichloroethane trichloroethylene 1,2,3-trichloropropane 1,1,2-trichlorotrifluoroethane (Freon 113) tridecane triethylamine triglyme (triethylene glycol dimethyl ether) 1,2,3-trimethylbenzene (hemimellitene) 1,2,4-trimethylbenzene (pseudocumene) 1,3,5-trimethylbenzene (mesitylene) undecane vinyl acetate m-xylene o-xylene p-xylene Retention Time 13.30 15.03 11.40 5.09 7.90 5.41 7.99 6.95 14.72 12.36 14.39 9.18 6.85 18.24 5.29 4.37 4.09 8.18 13.44 7.56 11.81 6.34 13.69 21.34 3.96 18.56 16.07 16.07 16.07 7.03 8.32 14.26 14.26 11.56 11.42 11.90 16.26 17.12 25.84 21.85 18.50 12.10 8.93 17.81 17.20 17.31 14.91 5.02 7.17 3.75 5.98 6.17 8.55 8.93 11.35 6.34 7.85 10.20 10.80 15.60 20.26 16.60 15.09 8.68 20.02 20.42 10.80 11.94 9.18 4.09 20.85 9.35 2.68 9.39 14.40 6.21 8.93 13.90 20.15 8.54 14.68 9.35 6.72 3.50 3.56 17.42 21.38 Compound 1,3-butanediol 1,4-butanediol 2,3-butanediol 2,3-butanedione (diacetal) 1-butanol 2-butanone (MEK; methyl ethyl ketone) 2-buten-1-ol (crotyl alcohol) 3-buten-1-ol 2-butoxyethanol (butyl cellosolve) butyl acetate butyl ether butyl ethyl ether butyl methyl ether butylbenzene butyraldehyde iso-butyraldehyde carbon disulfide carbon tetrachloride chlorobenzene 1-chlorobutane chlorodibromomethane chloroform 1-chlorohexane 1-chloro-4-nitrobenzene 3-chloropropene (allyl chloride) 4-chlorostyrene 2-chlorotoluene 3-chlorotoluene 4-chlorotoluene crotonaldehyde cyclohexane cyclohexanol cyclohexanone cyclopentanol cyclopentanone 2,4-dimethyl-3-pentanone (diisopropyl ketone) 2,6-dimethyl-4-heptanone (diisobutyl ketone) decane 1,10-decanediol 1-decanol 1,2-dibromo-3-chloropropane (DBCP) 1,2-dibromoethane (EDB) dibromomethane 1,2-dichlorobenzene 1,3-dichlorobenzene 1,4-dichlorobenzene trans-1,4-dichloro-2-butene 1,1-dichloroethane 1,2-dichloroethane (ethylene dichloride) 1,1-dichloroethylene (vinylidine chloride) cis-1,2-dichloroethylene trans-1,2-dichloroethylene 1,1-dichloropropane 1,2-dichloropropane 1,3-dichloropropane 2,2-dichloropropane 1,1-dichloropropene cis-1,3-dichloropropene trans-1,3-dichloropropene diethylene glycol diethylene glycol monobutyl ether diethylene glycol monoethyl ether diethylene glycol monomethyl ether diglyme (diethylene glycol dimethyl ether) 1,3-diisopropylbenzene 1,4-diisopropylbenzene DMF (dimethylformamide) DMSO (methylsulfoxide) 1,4-dioxane 1,3-dioxolane dodecane epichlorohydrin ethanol 2-ethoxyethanol (cellosolve) 2-ethoxyethyl acetate ethyl acetate ethyl acrylate ethylbenzene ethyl benzoate ethylene glycol ethylene glycol monobutyl ether ethylene glycol monoethyl ether ethylene glycol monomethyl ether ethyl ether ethyl formate 2-ethyl-1-hexanol -ethylphenethyl alcohol Retention Time 21.71 9.42 3.50 8.36 3.23 3.36 12.54 13.25 9.10 7.56 14.52 9.58 21.03 16.13 14.61 14.54 14.26 14.19 13.88 16.13 16.02 21.46 26.88 11.88 6.25 19.17 13.81 12.06 11.97 11.60 11.52 13.81 13.51 12.76 12.89 17.87 3.75 13.97 6.74 11.30 17.31 9.27 19.31 3.22 7.88 6.21 15.43 4.68 5.36 2.15 6.74 3.79 18.76 10.30 7.14 10.17 7.60 6.17 11.33 14.81 3.85 2.44 15.55 13.46 10.59 10.22 10.93 11.90 18.03 6.78 17.21 16.62 16.99 5.06 13.62 18.84 14.95 24.33 20.11 18.56 20.02 20.72 21.05 16.80 12.48 22.73 18.21 Compound -ethylphenethyl alcohol ethyl propionate ethyl vinyl ether fluorobenzene fluorotrichloromethane (Freon 11) furan furfural furfuryl alcohol glycidol glyme (propylene glycol dimethyl ether) heptanal heptane 1,7-heptanediol 1-heptanol 2-heptanol 3-heptanol 2-heptanone 3-heptanone 4-heptanone trans-2-hepten-1-ol cis-4-hepten-1-ol hexachloro-1,3-butadiene hexadecane hexanal hexane 1,6-hexanediol 1-hexanol 2-hexanol 3-hexanol 2-hexanone 3-hexanone cis-2-hexen-1-ol cis-3-hexen-1-ol 4-hexen-3-one 4-hydroxy-4-methyl-2-pentanone iodobenzene iodomethane iso-amyl acetate iso-butanol iso-butyl acetate iso-butylbenzene iso-octane isophorone iso-propanol iso-propyl acetate iso-propyl ether iso-propylbenzene (cumene) methacrolein methacrylonitrile methanol 2-methoxyethanol (methyl cellosolve) methyl acetate methyl benzoate 2-methyl-1-butanol (active amyl alcohol) 2-methyl-2-butanol (tert-amyl alcohol) 3-methyl-1-butanol (iso-amyl alcohol) 3-methyl-2-butanone 2-methyl-3-buten-2-ol 3-methyl-2-buten-1-ol 2-methylbutyl acetate methylene chloride methyl formate 5-methyl-3-heptanone 5-methyl-2-hexanone 2-methyl-3-pentanone 4-methyl-2-pentanone (MIBK; methyl isobutyl ketone) 4-methyl-2-pentanol 4-methyl-3-penten-3-one -methylphenyl alcohol methyl propionate 1-methyl-2-pyrrolidone -methylstyrene 4-methylstyrene methyl tert-butyl ether (MTBE) morpholine nonanal nonane 1,9-nonanediol 1-nonanol nitrobenzene 2-nitrotoluene 3-nitrotoluene 4-nitrotoluene octanal octane 1,8-octanediol 1-octanol
GC and GC/MS
DB-1 - Alphabetical Order

Column: P/N: Oven: Carrier: Injector: Detector: DB-1 30 m x 0.53 mm id, 3 m 125-1034 40C for 5 min 40-260C at 10/min Helium, constant pressure at 30 cm/sec (40C) Split 1:10, 250C FID, 300C
Retention Time 10.21 2.16 16.87 3.05 2.87 18.13 2.98 19.16 3.43 9.05 6.00 14.04 15.88 8.00 16.26 17.42 27.72 15.54 4.79 9.22 3.75 14.20
Compound acetal (acetaldehyde diethyl acetal) acetaldehyde acetic acid acetone acetonitrile acetophenone acrolein acrylic acid acrylonitrile allyl ether allyl ethyl ether amyl acetate benzaldehyde benzene benzyl acetate benzyl alcohol benzyl ether bromobenzene bromochloromethane bromodichloromethane bromoethane bromoform
342

Retention Time 16.46 16.46 26.38 25.51 8.76 3.51 17.17 8.66 11.11 9.10 9.10 8.46 8.80 8.54 11.11 8.40 5.09 18.51 20.92 21.36 9.95 4.44 4.09 3.11 18.18 4.43 4.57 9.47 16.07 21.91 9.05 9.90 6.48 12.25 10.21 5.80 10.91 17.37 14.55 18.24 8.68 3.72 9.32 16.99 6.85 18.97 23.35 13.43 14.67 12.66 24.06 9.35 6.75 18.23 18.23 18.50 11.33 21.28 20.63 19.85 7.56 11.00 9.22 14.84 4.09 22.51 8.93 20.75 17.61 16.99 16.41 19.07 4.09 14.11 14.69 14.11 Compound 2-octanone 3-octanone pentachlorobenzene pentadecane (valeraldehyde) pentane 1,5-pentanediol 2,3-pentanedione 1-pentanol 2-pentanol 3-pentanol 2-pentanone 3-pentanone 1-penten-3-ol 2-penten-1-ol 1-penten-3-one (ethyl vinyl ketone) 3-penten-2-one (methyl vinyl ketone) pentyl ether 2-phenoxyethanol 4-phenyl-2-butanone (benzyl acetone) 1,3-propanediol 1-propanol 2-propen-1-ol (allyl alcohol) propionaldehyde propionic acid propionitrile 2-propyn-1-ol (propargyl alcohol) propyl acetate propylbenzene propyl benzoate propyl ether propylene glycol (1,2-propanediol) propyl formate propyl propionate pyridine sec-butanol sec-butyl acetate sec-butylbenzene styrene styrene oxide tert-amyl methyl ether tert-butanol tert-butyl acetate tert-butylbenzene tert-butyl ethyl ether 4-tert-butyltoluene 1,2,4,5-tetrachlorobenzene 1,1,1,2-tetrachloroethane 1,1,2,2-tetrachloroethane tetrachloroethylene tetradecane tetrahydropyran THF (tetrahydrofuran) m-tolualdehyde o-tolualdehyde p-tolualdehyde toluene 1,2,3-trichlorobenzene 1,2,4-trichlorobenzene 1,3,5-trichlorobenzene 1,1,1-trichloroethane 1,1,2-trichloroethane trichloroethylene 1,2,3-trichloropropane 1,1,2-trichlorotrifluoroethane (Freon 113) tridecane triethylamine triglyme (triethylene glycol dimethyl ether) 1,2,3-trimethylbenzene (hemimellitene) 1,2,4-trimethylbenzene (pseudocumene) 1,3,5-trimethylbenzene (mesitylene) undecane vinyl acetate m-xylene o-xylene p-xylene
DB-WAX - Alphabetical Order

Column: P/N: Oven: DB-WAX 30 m x 0.53 mm id, 1 m 125-7032 40C for 5 min 40-230C at 10/min 230C for 7 min Helium, constant pressure at 34 cm/sec (40C) Split 1:10, 250C FID, 300C
Retention Time 17.06 17.00 9.10 7.20 2.65 7.84 4.38 9.71 4.95 12.38 3.99 4.56 11.27 12.78 23.91 21.74 19.48 19.06 6.04 15.47 16.20 7.94 12.03 9.65 7.09 15.25 19.21 12.23 11.69 6.46 13.11 14.31 5.03 7.87 11.13 20.27 19.47 16.24 13.11 12.24 2.13 3.78 17.41 23.10 24.12 6.93 2.39 7.72 2.13 3.27 18.32 19.90 17.25 6.04 12.32 2.65 27.26 16.91 14.71 14.29 12.27 11.66 11.06 17.77 17.67 17.84 18.70 10.07 25.75 2.05 15.31 12.95 12.49 10.04 9.34 16.31 15.87 12.55 15.70 18.30 3.46 10.96 10.31 8.36 13.47 3.54 2.44
Compound 1,4-dichlorobenzene trans-1,4-dichloro-2-butene 1,1-dichloroethane 1,2-dichloroethane (ethylene dichloride) 1,1-dichloroethylene (vinylidine chloride) cis-1,2-dichloroethylene trans-1,2-dichloroethylene 1,1-dichloropropane 1,2-dichloropropane 1,3-dichloropropane 2,2-dichloropropane 1,1-dichloropropene cis-1,3-dichloropropene trans-1,3-dichloropropene diethylene glycol diethylene glycol monobutyl ether diethylene gycol monoethyl ether diethylene glycol monomethyl ether diglyme (diethylene glycol dimethyl ether) 1,3-diisopropylbenzene 1,4-diisopropylbenzene 2,4-dimethyl-3-pentanone (diisopropyl ketone) 2,6-dimethyl-4-heptanone (diisobutyl ketone) 1,4-dioxane 1,3-dioxolane DMF (dimethylformamide) DMSO (methyl sulfoxide) dodecane epichlorohydrin ethanol 2-ethoxyethanol (cellosolve) 2-ethoxyethyl acetate ethyl acetate ethyl acrylate ethylbenzene ethyl benzoate ethylene glycol ethylene glycol monobutyl ether ethylene glycol monoethyl ether ethylene glycol monomethyl ether ethyl ether ethyl formate 2-ethyl-1-hexanol -ethylphenethyl alcohol -ethylphenethyl alcohol ethyl propionate ethyl vinyl ether fluorobenzene fluorotrichloromethane (Freon 11) furan furfural furfuryl alcohol glycidol glyme (propylene glycol dimethyl ether) heptanal heptane 1,7-heptanediol 1-heptanol 2-heptanol 3-heptanol 2-heptanone 3-heptanone 4-heptanone trans-2-hepten-1-ol cis-4-hepten-1-ol hexachloro-1,3-butadiene hexadecane hexanal 1,6-hexanediol hexane 1-hexanol 2-hexanol 3-hexanol 2-hexanone 3-hexanone cis-2-hexen-1-ol cis-3-hexen-1-ol 4-hexen-3-one 4-hydroxy-4-methyl-2-pentanone iodobenzene iodomethane iso-amyl acetate iso-butanol iso-butyl acetate iso-butylbenzene iso-butyraldehyde iso-octane
Carrier: Injector: Detector:
Retention Time 5.09 2.47 16.87 3.60 8.12 20.13 4.10 19.61 7.81 6.04 3.05 10.96 17.25 6.46 19.55 21.01 22.82 30.41 15.47 9.26 11.76 2.95 17.00 20.96 23.14 18.70 7.44 11.48 5.35 12.95 12.02 16.31 9.85 14.81 6.97 3.27 2.80 4.72 2.65 4.85 13.00 3.99 14.52 8.58 9.10 23.97 3.46 18.19 14.71 14.90 14.90 9.07 2.27 16.31 14.61 14.57 12.46 7.63 34.11 21.12 20.57 13.80 11.98 17.80 16.60
Compound acetal (acetaldehyde diethyl acetal) acetaldeyde acetic acid acetone acetonitrile acetophenone acrolein acrylic acid acrylonitrile allyl ether allyl ethyl ether amyl acetate benzaldehyde benzene benzonitrile benzyl acetate benzyl alcohol benzyl ether bromobenzene bromochloromethane bromodichloromethane bromoethane bromoform 1,3-butanediol 1,4-butanediol 2,3-butanediol 2,3-butanedione (diacetal) 1-butanol 2-butanone (MEK; methyl ethyl ketone) 2-buten-1-ol (crotyl alcohol) 3-buten-1-ol 2-butoxyethanol (butyl cellosolve) butyl acetate butylbenzene butyl ether butyl ethyl ether butyl methyl ether butyraldehyde carbon disulfide carbon tetrachloride chlorobenzene 1-chlorobutane chlorodibromomethane chloroform 1-chlorohexane 1-chloro-4-nitrobenzene 3-chloropropene (allyl chloride) 4-chlorostyrene 2-chlorotoluene 3-chlorotoluene 4-chlorotoluene crotonaldehyde cyclohexane cyclohexanol cyclohexanone cyclopentanol cyclopentanone decane 1,10-decanediol 1-decanol 1,2-dibromo-3-chloropropane (DBCP) 1,2-dibromoethane (EDB) dibromomethane 1,2-dichlorobenzene 1,3-dichlorobenzene
GC and GC/MS
343

Retention Time 19.47 6.28 5.32 12.13 3.27 4.83 7.54 5.40 12.25 3.78 19.70 12.73 8.43 12.73 6.15 9.11 14.82 12.05 6.18 2.85 12.38 11.40 7.94 8.19 11.89 11.26 22.00 5.54 15.13 15.61 20.71 2.30 13.62 21.41 22.62 21.92 23.14 16.05 4.97 31.29 19.80 14.29 3.22 29.06 18.40 14.20 13.66 25.09 17.28 7.46 1.89 24.43 9.49 13.54 10.94 10.66 7.44 7.44 11.77 14.65 8.56 6.61 12.66 26.14 22.76 18.97 9.11 10.78 3.25 18.18 8.72 7.38 12.86 21.46 18.96 3.05 5.93 9.17 15.16 12.44 8.77 7.69 13.64 13.80 19.46 3.27 5.54 Compound isophorone iso-propanol iso-propyl acetate iso-propylbenzene (cumene) iso-propyl ether methacrolein methacrylonitrile methanol 2-methoxyethanol (methyl cellosolve) methyl acetate methyl benzoate 2-methyl-1-butanol (active amyl alcohol) 2-methyl-2-butanol (tert-amyl alcohol) 3-methyl-1-butanol (iso-amyl alcohol) 3-methyl-2-butanone 2-methyl-3-buten-2-ol 3-methyl-2-buten-1-ol 2-methylbutyl acetate methylene chloride methyl formate 5-methyl-3-heptanone 5-methyl-2-hexanone 2-methyl-3-pentanone 4-methyl-2-pentanone (MIBK; methyl isobutyl ketone) 4-methyl-2-pentanol 4-methyl-3-penten-3-one -methylphenyl alcohol methyl propionate -methylstyrene 4-methylstyrene 1-methyl-2-pyrrolidone methyl tert-butyl ether (MTBE) morpholine nitrobenzene 2-nitrotoluene 3-nitrotoluene 4-nitrotoluene nonanal nonane 1,9-nonanediol 1-nonanol octanal octane 1,8-octanediol 1-octanol 2-octanone 3-octanone pentachlorobenzene pentadecane pentanal (valerlaldehyde) pentane 1,5-pentanediol 2,3-pentanedione 1-pentanol 2-pentanol 3-pentanol 2-pentanone 3-pentanone 1-penten-3-ol 2-penten-1-ol 1-penten-3-one (ethyl vinyl ketone) 3-penten-2-one (methyl vinyl ketone) pentyl ether 2-phenoxyethanol 4-phenyl-2-butanone (benzyl acetone) 1,3-propanediol 1-propanol 2-propen-1-ol (allyl alcohol) propionaldehyde propionic acid propionitrile propyl acetate propylbenzene propyl benzoate propylene glycol (1,2-propanediol) propyl ether propyl formate propyl propionate 2-propyn-1-ol (propargyl alcohol) pyridine sec-butanol sec-butyl acetate sec-butylbenzene styrene styrene oxide tert-amyl methyl ether tert-butanol Retention Time 5.40 13.41 2.47 15.13 22.08 13.80 17.73 8.58 15.75 5.83 4.45 19.77 19.73 20.13 9.06 21.25 20.03 18.19 4.85 13.80 7.84 17.06 2.13 14.09 3.26 20.77 15.29 14.27 13.55 10.10 5.03 11.44 12.39 11.30 Compound tert-butyl acetate tert-butylbenzene tert-butyl ethyl ether 4-tert-butyltoluene 1,2,4,5-tetrachlorobenzene 1,1,1,2-tetrachloroethane 1,1,2,2-tetrachloroethane tetrachloroethylene tetradecane tetrahydropyran HF (tetrahydrofuran) m-tolualdehyde o-tolualdehyde p-tolualdehyde toluene 1,2,3-trichlorobenzene 1,2,4-trichlorobenzene 1,3,5-trichlorobenzene 1,1,1-trichloroethane 1,1,2-trichloroethane trichloroethylene 1,2,3-trichloropropane 1,1,2-trichlorotrifluoroethane (Freon 113) tridecane triethylamine triglyme (triethylene glycol dimethyl ether) 1,2,3-trimethylbenzene (hemimellitene) 1,2,4-trimethylbenzene (pseudocumene) 1,3,5-trimethylbenzene (mesitylene) undecane vinyl acetate m-xylene o-xylene p-xylene
GC and GC/MS
344

Pesticide Retention Data
Pesticide Elution Order Using Low Bleed Phases

Run Conditions: Columns: 30 m x 0.25 mm id, 0.25 m Carrier Gas: Helium at 35 cm/sec, measured at 50C Oven: 50C for 1 min 50-100C at 25/min 100-300C at 5/min (DB-1701 ramped to 280C) 300C for 5 min (DB-1701 held at 280C for 10 min)
Alphabetical Order By Analyte

Compound Alachlor Aldrin Aspon Atrazine Azinphos-ethyl Azinphos-methyl -BHC -BHC -BHC -BHC Bolstar 1-Bromo-2-nitrobenzene (IS) 2-Bromobiphenyl (SS) Captafol Captan Carbophenothion -Chlordane -Chlordane Chlorfenvinphos 4-Chloro-3-nitrobenzotrifluoride (SS) Chlorobenzilate Chloroneb Chloropropylate Chlorothalonil Chlorpyrifos Chlorpyrifos-methyl Coumaphos Crotoxyphos Dacthal p,p-DDD p,p-DDE p,p-DDT Demeton-O Demeton-S Diallate A Diallate B Diazinon 1,2-Dibromo-3-chloropropane DB-5ms 24.29 25.99 25.72 21.11 37.51 36.28 20.01 21.12 21.46 22.70 32.16 11.50 17.31 33.91 27.98 32.56 28.54 29.06 27.61 7.66 31.28 15.53 31.28 22.16 25.84 23.86 38.74 28.16 26.11 31.62 29.97 33.07 17.91 20.52 19.88 20.26 21.99 6.63 DB-XLB 25.84 27.33 26.69 22.65 39.94 38.83 21.83 24.95 23.37 25.98 33.89 12.73 18.49 36.35 30.15 34.49 30.72 30.90 29.34 8.55 32.82 16.87 32.92 26.44 27.52 25.64 41.40 29.48 27.55 33.93 31.82 35.12 18.97 21.83 20.87 21.35 23.05 7.11 DB-35ms 27.40 28.28 28.11 24.82 43.31 42.60 23.50 26.62 25.32 27.83 36.25 14.87 20.24 39.46 33.20 36.69 31.77 32.19 31.47 8.83 34.03 18.68 34.48 28.06 29.31 27.79 44.01 32.13 29.13 35.60 33.20 36.71 20.58 24.03 21.97 22.40 24.59 8.05 DB-17ms 27.91 28.54 27.55 24.50 43.27 42.75 23.83 26.70 25.73 28.08 35.94 15.66 21.01 40.31 34.14 36.26 31.91 32.43 31.15 8.59 34.27 19.37 34.85 28.08 28.86 27.55 43.52 32.09 29.61 35.92 33.53 37.05 20.13 23.67 22.31 22.71 24.21 8.47 31.89 28.82 34.60 31.50 35.37 20.49 24.05 21.42 22.10 24.16 7.90 DB-1701 27.59 26.79 28.22 25.12 48.74 45.86 23.06 24.84 27.97 28.89 34.96 14.68 18.62 40.40 32.25 35.48 30.91 31.21 31.02 10.00 33.78 17.92 33.98 27.73 28.36 26.70
GC and GC/MS
345

Pesticide Retention Data
Alphabetical Order By Analyte (Continued)
Compound ,-Dibromo-m-xylene Dibutylchlorendate (SS) Dichlofenthion Dichlorvos Dicrotophos Dieldrin Dimethoate Dioxathion Disulfoton Endosulfan I Endosulfan II Endosulfan sulfate Endrin Endrin aldehyde Endrin ketone EPN Ethion Ethoprop Ethylparathion Famphur DB-5ms 16.72 36.32 23.64 9.31 19.12 30.14 20.52 21.41 22.37 29.06 31.39 32.88 31.00 31.96 34.68 34.86 31.55 18.47 26.17 32.38 25.19 31.25 26.02 21.76 24.52 27.59 20.12 11.42 10.25 27.17 35.37 26.57 36.17 25.62 30.01 35.22 24.14 13.50 37.09 19.55 18.86 29.18 21.22 38.62 38.89 31.00 19.79 34.73 DB-XLB 18.27 37.75 25.07 9.93 20.58 32.03 22.32 22.78 23.68 30.95 34.11 35.74 32.92 34.57 37.26 37.04 33.09 19.60 28.09 34.38 26.96 33.31 27.62 23.19 25.98 29.32 22.13 11.94 11.10 28.71 37.59 28.71 38.15 26.96 31.47 37.05 26.14 14.48 39.12 21.15 20.15 31.15 23.47 40.27 40.57 32.67 21.02 37.24 DB-35ms 20.60 38.67 26.58 11.53 23.77 33.59 25.80 25.53 25.53 32.37 36.04 37.84 34.89 37.00 40.23 39.61 35.39 21.42 29.94 37.69 29.47 36.44 30.25 25.29 26.92 30.76 22.91 12.08 12.74 30.10 39.54 30.35 40.73 29.31 32.94 39.54 28.56 16.72 40.67 24.70 22.72 31.91 24.84 42.12 42.42 34.29 22.85 40.83 DB-17ms 21.40 38.74 26.02 11.39 23.98 33.90 26.02 25.49 25.09 32.61 36.36 38.13 35.35 37.52 40.99 39.26 35.11 21.02 29.36 37.72 29.22 36.36 30.22 25.03 27.11 31.07 23.01 12.25 12.54 30.48 39.91 30.68 40.55 29.13 32.22 40.31 28.22 16.69 40.99 24.97 22.70 31.91 25.08 42.53 42.80 34.68 22.45 40.91 40.94 29.20 31.89 38.91 28.57 17.56 37.96 26.50 22.41 31.29 23.64 42.25 46.52 32.51 22.33 42.38 DB-1701 19.41 39.65 26.02 12.45 24.66 32.16 26.70 24.80 24.95 30.72 34.97 38.91 32.97 37.01 41.57 41.19 34.85 21.29 30.10 39.69 29.36 37.10 29.07 24.41 25.69 29.68 21.03 11.60 15.46 28.44
GC and GC/MS
Fenitrothion Fensulfothion Fenthion Fonofos Heptachlor Heptachlor epoxide Hexachlorobenzene Hexachlorocyclopentadiene Hexamethylphosphoramide Isodrin Kelthane Kelthane Decomp. Product Leptophos Malathion Merphos Methoxychlor Methylparathion Mevinphos Mirex Monocrotophos Naled trans-Nonachlor Pentachloronitrobenzene (IS) cis-Permethrine trans-Permethrine Perthane Phorate Phosmet
346

Pesticide Retention Data/Environmental-Hydrocarbons
Alphabetical Order By Analyte (Continued)
Compound Phosphamidon Propachlor Ronnel Simazine Stirophos Sulfotep TEPP Terbufos Terrazole Tetrachloro-m-xylene (SS) Thionazin Tokuthion Trichloronate Trifluralin Tri-o-cresylphosphate DB-5ms 23.56 17.88 24.58 20.91 28.66 19.37 16.76 21.64 14.17 18.12 17.72 29.55 26.51 19.30 36.64 DB-XLB 23.40 19.32 26.14 22.65 30.50 20.42 17.91 22.78 15.12 19.81 18.97 31.19 28.09 20.35 38.40 DB-35ms 27.79 21.17 27.95 25.05 32.94 22.46 20.69 24.23 16.60 20.24 21.26 32.83 29.31 19.98 40.93 DB-17ms 27.72 21.81 27.55 24.87 32.69 22.27 20.74 23.67 17.25 20.32 21.12 32.22 28.59 19.47 40.83 DB-1701 28.85 21.74 27.10 25.23 32.27 22.56 21.98 23.91 15.86 18.70 20.85 31.67 28.57 22.15 42.62
Methyl tert-butyl ether (MTBE) PID, Extended 8020 Analysis

Column:
P/N: Carrier: Oven:
Methyl tert-butyl ether (MTBE) FID, Extended 8020 Analysis

Column:
15. 1,3,5-Trimethylbenzene 16. 1,2,4-Trimethylbenzene 17. 1,2,3-Trimethylbenzene 18. Naphthalene SS - Surrogate Standard P/N: Carrier: Oven:
DB-MTBE
30 m x 0.45 mm id, 2.55 m 124-0034 Helium at 10 mL/min 35C for 4 min 35-200C at 20/min 200C for 5 min Purge and trap (O.I.A. 4560) J&W LVI (Low Volume Injector), 150C 40 ppb per component in 5 mL water Tenax only 175C 180C for 3 min FID (O.I.A. 4410), 200C 15. 1,3,5-Trimethylbenzene 16. 1,2,4-Trimethylbenzene 17. 1,2,3-Trimethylbenzene 18. Naphthalene SS - Surrogate Standard
GC and GC/MS
DB-MTBE
30 m x 0.45 mm id, 2.55 m 124-0034 Helium at 10 mL/min 35C for 4 min 35-200C at 20/min 200C for 5 min Purge and trap (O.I.A. 4560) J&W LVI (Low Volume Injector), 150C 40 ppb per component in 5 mL water Tenax only 175C 180C for 3 min PID (O.I.A. 4430), 200C
Injector:
Injector:
Trap: Preheat: Desorb: Detector:
Trap: Preheat: Desorb: Detector:
1. Methyl-tert-butyl ether (MTBE) 2. 2-Methylpentane 3. 3-Methylpentane 4. Diisopropyl ether (DIPE) 5. Ethyl-tert-butyl ether (ETBE) 6. Benzene 7. tert-Amyl methyl ether (TAME) 8. ,,-Trifluorotoluene (SS) 9. Toluene 10. Ethylbenzene 11. m-Xylene 12. p-Xylene 13. o-Xylene 14. Cumene
1. Methyl-tert-butyl ether (MTBE) 2. 2-Methylpentane 3. 3-Methylpentane 4. Diisopropyl ether (DIPE) 5. Ethyl-tert-butyl ether (ETBE) 6. Benzene 7. tert-Amyl methyl ether (TAME) 8. ,,-Trifluorotoluene (SS) 9. Toluene 10. Ethylbenzene 11. m-Xylene 12. p-Xylene 13. o-Xylene 14. Cumene
C539
C540
347

Environmental-Hydrocarbons
Unleaded Gasoline
Column:
P/N: Carrier: Oven:
DB-VRX
30 m x 0.45 mm id, 2.55 m 124-1534 Helium at 109 cm/sec (10.4 mL/min), measured at 40C 40C for 2 min 40-200C at 12/min 200C for 5 min Purge and trap (O.I.A. 4460A) J&W LVI (Low Volume Injector) 115 ppb gasoline in 5 mL water BTEX (Supelco) at 50C during purge 1 min at 270C PID (O.I.A. 4430), 200C FID, 250C
Injector:
Trap: Desorb: Detector A: Detector B:
1. 3-Methylpentane 2. Benzene 3. Isooctane 4. Toluene 5. Ethylbenzene 6. m,p-Xylene 7. o-Xylene 8. 1,2,4-Trimethylbenzene 9. Naphthalene
FID
PID
GC and GC/MS
C174
Diesel Fuel
Column:
P/N: Carrier: Oven:
DB-5ms
30 m x 0.53 mm id, 1.5 m 125-5532 Helium at 48.5 cm/sec, measured at 60C 60C for 2 min 60-300C at 12/min 300C for 10 min Megabore Direct, 280C 1 L injection in hexane FID, 250C Nitrogen makeup gas at 30 mL/min 1. n-C10, Decane 2. n-C12, Dodecane 3. n-C14, Tetradecane 4. n-C16, Hexadecane 5. n-C18, Octadecane 6. n-C20, Eicosane 7. n-C22, Docosane 8. n-C24, Tetracosane 9. n-C26, Hexacosane 10. n-C28, Octacosane
Injector: Detector:
Diesel Fuel Standard 50 ng/component
Diesel Fuel 0.6 mg/mL
C175
348

Environmental-Pesticides and Herbicides
CLP Pesticides
Column:
P/N: 1. Tetrachloro-m-xylene (SS) 2. -BHC 3. -BHC 4. -BHC 5. Heptachlor 6. -BHC 7. Aldrin 8. Heptachlor epoxide 9. -Chlordane 10. -Chlordane 11. Endosulfan I 12. 4,4-DDE 13. Dieldrin 14. Endrin 15. 4,4-DDD 16. Endosulfan II 17. 4,4-DDT 18. Endrin aldehyde 19. Endosulfan sulfate 20. Methoxychlor 21. Endrin ketone 22. Decachlorobiphenyl (SS) SS - Surrogate Standard 12 8 1 5 6 18 4 14 17 19 22 9 10 11 15 16 21 13
DB-35ms
30 m x 0.32 mm id, 0.25 m film 123-3832
Column:
P/N: Carrier: Oven:
DB-XLB
30 m x 0.32 mm id, 0.5 m film 123-1236 Helium at 45 cm/sec (EPC in constant flow mode) 110C for 0.5 min 110-320C at 15 C/min 320C for 2 min Splitless, 250C 30 sec purge activation time 50 pg per component ECD, 350C Nitrogen makeup gas (column + makeup flow = 30 mL/min constant flow) 2
Injector:
Detector:
DB-35ms
3 7
GC and GC/MS
20
C784-2
10 Time (min)
12
14
16
DB-XLB
2
Complete resolution and confirmation of 22 CLP Pesticides in under 16 minutes!

3 7 8 9 10 11 15 6 5 4 17 14 18 19 22 12 13
16 21
20
10 Time (min)
12
14
16
C783
349

Organochlorine Pesticides (EPA Method 8081A)
Column:
P/N: Carrier: Oven: 9. Tetrachloro-m-xylene (SS) 10. , -Dibromo-m-xylene 11. Propachlor 12. Di-allate A 13. Di-allate B 14. Hexachlorobenzene 15. -BHC 16. Pentachloronitrobenzene (IS) 17. -BHC 18. -BHC 19. Heptachlor 20. Alachlor 21. -BHC 22. Chlorothalonil 23. Aldrin 24. Dacthal 25. Isodrin 26. Kelthane 27. Heptachlor epoxide 28. -Chlordane 29. trans-Nonachlor 30. -Chlordane 31. Endosulfan I 32. Captan 33. p,p-DDE 34. Dieldrin 35. Chlorobenzilate 36. Perthane 37. Chloropropylate 38. Endrin 39. p,p-DDD 40. Endosulfan II 41. p,p-DDT 42. Endrin aldehyde 43. Endosulfan sulfate 44. Dibutylchlorendate (SS) 45. Captafol 46. Methoxychlor 47. Endrin ketone 48. Mirex 49. cis-Permethrin 50. trans-Permethrin * Breakdown Products SS - Surrogate Standard IS - Internal Standard Standards used were a composite of individual solutions supplied courtesy of Accustandard Inc., 25 Science Park, New Haven, CT 06511, 800-442-5290.
DB-35ms
30 m x 0.25 mm id, 0.25 m 122-3832 Helium at 35 cm/sec, measured at 50C 50C for 1 min 50-100C at 25/min 100-300C at 5/min 300C for 5 min Splitless, 250C 30 sec purge activation time 1 L of 35 g/mL composite 8081A standards, Accustandard Inc. MSD, 300C transfer line full scan at m/z 50-500
Injector:
Detector:
1. 1,2-Dibromo-3-chloropropane 2. 4-Chloro-3-nitrobenzotrifluoride (SS) 3. Hexachloropentadiene 4. 1-Bromo-2-nitrobenzene (IS) 5. Terrazole 6. Chloroneb 7. Trifluralin 8. 2-Bromobiphenyl (SS)
GC and GC/MS
C512
Organochlorine Pesticides (EPA Method 8081A)

Column:
P/N: Carrier: Oven:
DB-5ms
30 m x 0.25 mm id, 0.25 m 122-5532 Helium at 35 cm/sec, measured at 50C 50C for 1 min 50-100C at 25/min 100-300C at 5/min 300C for 5 min Splitless, 250C 30 sec purge activation time 1 L of 35 g/mL composite 8081A standards, Accustandard Inc. MSD, full scan at m/z 50-500 300C transfer line
Injector:
Detector:
1. 1,2-Dibromo-3-chloropropane 2. 4-Chloro-3-nitrobenzotrifluoride (SS) 3. Hexachloropentadiene 4. 1-Bromo-2-nitrobenzene (IS) 5. Terrazole 6. Chloroneb 7. Trifluralin 8. 2-Bromobiphenyl (SS) 9. Tetrachloro-m-xylene (SS) 10. , Dibromo-m-xylene 11. Propachlor 12. Di-allate A 13. Di-allate B 14. Hexachlorobenzene 15. -BHC 16. Pentachloronitrobenzene (IS) 17. -BHC 18. -BHC 19. Heptachlor 20. Alachlor 21. -BHC
22. Chlorothalonil 23. Aldrin 24. Dacthal 25. Isodrin 26. Kelthane 27. Heptachlor epoxide 28. -Chlordane 29. trans-Nonachlor 30. Chlordane 31. Endosulfan I 32. Captan 33. p, p-DDE 34. Dieldrin 35. Chlorobenzilate 36. Perthane 37. Chloropropylate
38. Endrin 39. p, p-DDD 40. Endosulfan II 41. p, p-DDT 42. Endrin aldehyde 43. Endosulfan sulfate 44. Dibutylchlorendate (SS) 45. Captafol 46. Methoxychlor 47. Endrin ketone 48. Mirex 49. cis-Permethrin 50. trans-Permethrin * Breakdown Products SS - Surrogate Standard IS - Internal Standard
C494
350

EPA 508.1 Pesticides
Column:
P/N: 1. Hexachlorocyclopentadiene 2. Etridiazole 3. Chloroneb 4. Trifluralin 5. Propachlor 6. Hexachlorobenzene 7. -BHC 8. Atrazine 9. Pentachloronitrobenzene 10. Simazine 11. -BHC 12. -BHC 13. Heptachlor 14. Alachlor 15. -BHC 16. Chlorothalonil 17. Aldrin 18. Metribuzin 19. Metolachlor 20. DCPA
28 24 25 26 13 17 32 18 1 12 2 9,10 4 30 31 33 35
DB-35ms
30 m x 0.32 mm id, 0.25 m 123-3832
Column:
P/N: Carrier: Oven:
DB-XLB
30 m x 0.32 mm id, 0.50 m 123-1236 Helium at 45 cm/sec (EPC in constant flow mode) 75C for 0.5 min 75-300C at 10 C/min 300C for 2 min Splitless, 250C 30 sec purge activation time 50 pg per component 7 ECD, 350C Nitrogen makeup gas (column + makeup flow = 30 mL/ 6 min constant flow)
11
Injector:
Detector:
16 15
21. 4,4-Dibromobiphenyl 22. Heptachlor epoxide 23. Cyanazine 24. Chlordane 25. -Chlordane 26. Endosulfan I 27. 4,4-DDE 28. Dieldrin 29. Chlorobenzilate 30. Endrin 31. 4,4-DDD 32. Endosulfan II 33. 4,4-DDT 34. Endrin aldehyde 35. Endosulfan sulfate 36. Methoxychlor 37. cis-Permethrin 38. trans-Permethrin
21,22
DB-35ms
20
27
36 23 3 14 5 8 19 29 37 34 38
GC and GC/MS
C786
10
12
14
16 Time (min) 7 6
18
20
22
24
DB-XLB
8,11
15 16 24 20 13 17 22 26 32 35 33 28 27
25
1 4 2
18 12
30 31
21 23 3 5 8,10 14 19 29 34
36
38 37
10
12
14
C787
16 Time (min)
18
20
22
24
351

Chlorinated Pesticides: EPA Method 508
Column:
P/N: GC: Carrier: Oven:
HP-5ms
30 m x 0.25 mm id, 0.25 m 19091S-433 5890 EPC Helium, 24 psi, 45 cm/sec (80C) constant flow 80C for 1 min 80-180C at 30C/min 180-205C at 3C/min 205C for 4 min 205-290C at 20C/min 290C for 2 min Splitless, 1 L, 1 min purge delay ECD 320C, Nitrogen, 60 mL/min makeup gas 3 mL/min anode purge 6 5 10 13 8 9 15 17 1. Etridiazole 2. Chloroneb 3. Propachlor 4. Trifluralin 5. -BHC 6. Hexachlorobenzene 7. -BHC 8. -BHC 9. -BHC 10. Chlorothalonil 11. Heptachlor 12. Aldrin 13. DCPA 14. Heptachlor epoxide 15. -Chlordane 16. Endosulfan I 17. -Chlordane 18. Dieldrin 19. 4,4'-DDE 20. Endrin 21. Endosulfan II 22. Chlorobenzilate 23. 4,4'-DDD 24. Endrin aldehyde 25. Endosulfan sulfate 26. 4,4'-DDT 27. Methoxychlor 28. cis-Permethrin 29. trans-Permethrin 29
Injection: Detector:
4 1
12 7 11 25 14 16 18 19 27 21 20 26 28
2 3
23 24 22
GC and GC/MS
C1023
10 Time (min)
15
20
Organohalide Pesticides in Water, EPA Method 505

Column: HP-5MS
P/N: Carrier: Oven: 30 m x 0.25 mm id, 0.25 m 19091S-433 Helium, 2.0 m./min, constant flow, 42 cm/sec (22.4 psi at 80C) 1. Hexachlorocyclopentadiene 9. Heptachlor epoxide 80C for 1 min 2. Hexachlorobenzene 10. -Chlordane 80-175C at 30C/min 3. Simazine 11. -Chlordane 175C for 4 min 4. Atrazine 12. trans-Nonachlor 175- 215C at 6C/min 5. Lindane 13. Dieldrin 215C for 2 min 6. Heptachlor 14. Endrin 215- 290Cat 15C/min 7. Alachlor 15. cis-Nonachlor 290C for 5 min 8. Adrin 16. Methoxychlor Splitless, 250C, 1 min purge delay 1 L injection volume Sample: ECD, 300C 16 components EPA-505 targeted pesticides and 14 ppb Makeup gas: N2, 60 mL/min Aroclor 1260 in hexane. Anode purge 6 mL/min Concentration of pesticides: 50 ppb each except 1.25 ppm for atrazine and simazine. 2 4 3 5 8 7 6 1 9 11 10 12 14 13 15 16
Injector:
Detector:
C1038
10
15 Time (min)
20
352

Organochlorine Pesticides
Column:
P/N: Carrier: Oven: Injector: Detector:
DB-5
30 m x 0.53 mm id, 0.5 m 125-5037 Helium at 30 cm/sec (4.0 mL/min) 150-275C at 4/min 275C for 30 min Megabore Direct, 250C 0.7 L of 100 pg/L standard in isooctane ECD, 300C Nitrogen makeup gas at 30 mL/min
1. 2,4,5,6-Tetrachloro-m-xylene (IS) 2. -BHC 3. -BHC 4. -BHC 5. -BHC 6. Heptachlor 7. Aldrin 8. Heptachlor epoxide 9. -Chlordane 10. Endosulfan I 11. -Chlordane 12. Dieldrin 13. p,p-DDE 14. Endrin 15. Endosulfan II 16. p,p-DDD 17. Endrin aldehyde 18. Endosulfan sulfate 19. p,p-DDT 20. Endrin ketone 21. Methoxychlor 22. Decachlorobiphenyl (IS)
GC and GC/MS
C177
Column:
DB-608
C178
353

Column:
DB-1701
GC and GC/MS
C180
Column:
DB-35
C179
354

Organochlorine Pesticides, DB-5/1701P Dual Column
Columns: DB-5
30 m x 0.32 mm id, 0.25 m
DB-1701P
Guard Column: P/N: Carrier: Oven: 30 m x 0.32 mm id, 0.21 m 10 m x 0.53 mm id 3-way union 009-5007 Helium at 29.2 cm/sec, measured at 150C 60C for 0.5 min 60-140C at 20/min 140-280C at 11/min 280C for 23 min Splitless, 200C 2.0 L, 20-200 pg/L ECD, 325 Nitrogen makeup gas at 30 mL/min
Injector: Detector:
1. Etridiazole 2. Chloroneb 3. Propachlor 4. Tetrachloro-m-xylene (IS) 5. Trifluralin 6. -BHC 7. Hexachlorobenzene 8. -BHC 9. -BHC 10. Pentachloronitrobenzene 11. p,p'-Dichlorobiphenyl 12. -BHC 13. Heptachlor 14. Alachlor 15. Aldrin
16. Chlorpyrifos 17. DCPA 18. Isodrin 19. Heptachlor epoxide 20. Captan 21. -Chlordane 22. o,p-DDE 23. Endosulfan I 24. -Chlordane 25. Dieldrin 26. p,p-DDE 27. o,p-DDD 28. Endrin 29. Endosulfan II 30. Chlorobenzilate
31. p,p-DDD 32. o,p-DDT 33. Endrin aldehyde 34. Endrin ketone 35. Carbophenothion 36. p,p-DDT 37. Endosulfan sulfate 38. Hexabromobenzene (HBB) 39. Methoxychlor 40. Mirex 41. cis-Permethrin 42. trans-Permethrin 43. Decachlorobiphenyl (IS)
GC and GC/MS
Enlarged view of critical pair on DB-5
DB-5 DB-1701
Enlarged view of critical pairs on DB-1701 C176
355

Nitrogen/Phosphorus Containing Pesticides: EPA Method 507
Columns:
HP-5MS
30 m x 0.25 mm id, 0.25 m 19091S-433 5890 Series II Helium, 30 cm/sec (13.6 psi) pressure program 80-178C at 30C/min 178C for 4 min 178-205C at 2C/min 205-310C at 30C/min 310C for 4 min Splitless mode, 260C 1 min purge delay NPD, 290C Helium makeup gas at 30 mL/min
Injector: Detector:
1. Dichlorvos 2. EPTC 3. Butylate 4. Mevinphos 5. Vernolate 6. Pebulate 7. Tebuthiuron 8. Molinate 9. Ethoprop 10. Cycloate 11. Chlorpropham 12. Atraton 13. Simazine 14. Prometon 15. Atrazine
16. Propazine 17. Terbufos 18. Pronamide 19. Diazinon 20. Disulfoton 21. Terbacil 22. Metribuzin 23. Simetryn 24. Alachlor 25. Ametryn 26. Prometryn 27. Terbutryn 28. Bromacil 29. Metolachlor 30. Triademefon
41
31. MGK-264 32. Diphenamid 33. Stirofos 34. Butachlor 35. Fenamiphos 36. Napropamide 37. Tricyclazole 38. Merphos 39. Carboxin 40. Norflurazon 41. Hexazinone 42. Fenarimol 43. Fluridone
9 2 5 36 7 8
14 13 15 12 16
23 24 25,26
10
17 20 19 22
27 38 30
40
42
1 4 11 18 21 28
32 29 31
37 36 35 33 34 39
43
GC and GC/MS
C1025
10
15 Time (min)
20
25
Organophosphorous Pesticides (EPA Method 8141A)

Column:
P/N: Carrier: Oven:
DB-5ms
30 m x 0.25 mm id, 0.25 m 122-5532 Helium at 35 cm/sec, measured at 50C 50C for 1 min 50-100C at 25/min 100-300C at 5/min 300C for 5 min Splitless, 250C 2 30 sec purge activation time 1 L of 40 g/mL 8141A standards, Accustandard Inc. MSD, 300C transfer line full scan at m/z 50-500
Injector:
Detector:
Note: All standards used were supplied courtesy of Accustandard Inc., 25 Science Park, New Haven, CT 06511, 800-442-5290.
1 3
1. 4-Chloro-3-nitrotrifluoride 20. Fonofos 30. Aspon 2. Dichlorovos 21. Diazinon 31. Chlorpyrifos 3. Hexamethylphosphoramide 22. Disulfoton 32. Fenthion 4. Mevinphos 23. Phosphamidon 33. Ethyl parathion 5. TEPP 24. Dichlofenthion 34. Trichloronate 6. Thionazin 25. Chlorpyrifos-methyl 35. Chlorfenvinphos 7. Demeton-O 26. Methyl parathion 36. Crotoxyphos 8. Ethoprop 27. Ronnel 37. Stirophos 9. Naled 28. Fenitrothion 38. Tokuthion 10. Dicrotophos 29. Malathion 39. Merphos 11. Sulfotepp 40. Fensulfothion 21 12. Monocrotophos 41. Ethion 13. Phorate 42. Bolstar 22 14. Dimethoate 31 8 13 15. Demeton-S 20 16. Simazine 34 38 19 17. Atrazine 27 32 11 18. Dioxathion 30 7 24 19. Terbufos 6 36 39 37 29 4 17 25 26 35 14,15 23 33 12 16 28
9 18
43. Famphur 44. Carbophenothion 45. Phosmet 46. EPN 47. Leptophos 48. Azinphos methyl 49. Tri-o-cresyl phosphate 50. Azinphos ethyl 51. Coumaphos * Breakdown products
41
42 44 43 49 45 50 48 47 51
40
46
* 5 10 * * * *
10
C516
15
20 Time (min)
25
30
35
356

Organophosphorous Pesticides (EPA Method 8141A)
Column:
P/N: Carrier: Oven: 1. 4-Chloro-3-nitrotrifluoride 2. Dichlorvos 3. Hexamethylphosphoramide 4. Mevinphos 5. TEPP 6. Thionazin 7. Demeton-O 8. Ethoprop 9. Naled 10. Dicrotophos 11. Sulfotepp 12. Monocrotophos 13. Phorate 14. Dimethoate 15. Demeton-S 16. Simazine 17. Atrazine 18. Dioxathion 19. Terbufos 20. Fonofos 21. Diazinon 22. Disulfoton 23. Phosphamidon 24. Dichlofenthion 25. Chlorpyrifos-methyl 26. Methyl parathion 27. Ronnel 28. Fenitrothion 29. Malathion 30. Aspon 31. Chlorpyrifos 32. Fenthion 33. Ethyl parathion 34. Trichloronate 35. Chlorfenvinphos 36. Crotoxyphos 37. Stirophos 38. Tokuthion 39. Merphos 40. Fensulfothion 41. Ethion 42. Bolstar 43. Famphur 44. Carbophenothion 45. Phosmet 46. EPN 47. Leptophos 48. Azinphos methyl 49. Tri-o-cresyl phosphate 50. Azinphos ethyl 51. Coumaphos * Breakdown products
DB-35ms
30 m x 0.25 mm id, 0.25 m 122-3832 Helium at 35 cm/sec, measured at 50C 50C for 1 min 50-100C at 25/min 100-300C at 5/min 300C for 5 min Splitless, 250C 30 sec purge activation time 1 L of 40 g/mL 8141A standards, Accustandard Inc. MSD, full scan at m/z 50-500 300C transfer lin
Injector:
Detector:
Note: All standards used were supplied courtesy of Accustandard Inc., New Haven, CT
GC and GC/MS
C517
EPA 8151A Phenoxy Acid Herbicides - Methyl Derivatives

Column:
P/N:
DB-35ms
30m x 0.32 mm id, 0.25 m 123-3832
Column:
P/N: Carrier: Oven:
DB-XLB
30m x 0.32 mm id, 0.50 m 123-1236 Helium at 45 cm/sec (EPC in constant flow mode) 50C for 0.5 min 50-100C at 25C/min 100-320C at 12C/min 320C for 2 min Splitless, 250C 30 sec purge activation time 50 pg per component ECD, 350C Nitrogen makeup gas (column + makeup flow = 30 mL/min constant flow)
1
1. Dalapon 2. 3,5-Dichlorobenzoic acid 3. 4-Nitrophenol 4. Methyl-2,4-dichlorophenylacetate (SS) 5. Dicamba 6. MCPP 7. MCPA 8. 4,4-Dibromooctafluorobiphenyl (IS) 9. Dichloroprop 10. 2,4-D
11
11. Pentachlorophenol 12. 2,4,5-T,P 13. 2,4,5-T 14. Chloramben 15. Dinoseb 16. 2,4-DB 17. Bentazone 18. DCPA 19. Picloram 20. Acifluorofen
Injector:
Detector:
12 5 2 3 10
18 13 14 15 19 20
DB-35ms
4
16
17
8 Time (min)
10
12 11
14
16
DB-XLB
12 5 1 2 3 4 10 19 14 13 7 9 15 16 18 20
17
8 Time (min)
10
12
14
16
C785
357

Phenoxy Acid Herbicides
Column:
P/N: Carrier: Oven:
DB-1701
30 m x 0.32 mm id, 0.25 m 123-0732 Helium at 35 cm/sec, measured at 50C 50C for 1 min 50-160C at 10/min 160C for 3 min 160-260C at 10/min 260C for 5 min Splitless, 250C 45 sec purge activation time 1 L of 0.1 g/L standard in methanol FID, 300C Nitrogen makeup gas at 30 mL/min
TMS Esters
Injector:
Detector:
1. Dalapon 2. Dicamba 3. MCPP 4. MCPA 5. Dichloroprop 6. 2,4-D 7. Pentachlorophenol 8. Dinoseb 9. 2,4,5-TP 10. 2,4,5-T 11. 2,4-DB 12. Picloram
Methyl Esters
GC and GC/MS
C014
Herbicides
Column:
P/N: Carrier: Oven:
DB-XLB
30 m x 0.25 mm id, 0.25 m 122-1232 Helium at 32 cm/sec, measured at 50C 50C for 1 min 50-180C at 10/min 180-230C at 5/min 230-320C at 10/min 320C for 2 min Splitless, 250C 30 sec purge activation time 2 L x 10-50 ng/L solution in acetone MSD, 50-400 full scan transfer line 300C
Injector:
1. Monuron 2. Diuron 3. EPTC 4. Dichlobenil 5. Vernolate 6. Pebulate 7. Molinate 8. Sulfallate 9. Atraton 10. Prometon 11. Atrazine 12. Propazine
13. Simazine 14. Terbuthylazine 15. Pronamide 16. Secbumeton 17. Terbacil 18. Alachlor 19. Propanil 20. Ametryne 21. Prometryne 22. Simetryn 23. Metribuzin 24. Terbutryn
25. Metolachlor 26. Bromacil 27. Dacthal 28. Diphenamid 29. Butachlor 30. Napropamide 31. Carboxin 32. Tricyclazole 33. Norflurazon 34. Hexazinone 35. Difolotan 36. Fluridone *Impurity
Detector:
C414
358

Environmental-Pesticides and Herbicides/Environmental-Semivolatiles
Triazine Herbicides
Column:
P/N: Carrier: Oven:
Triazine Herbicides
Column:
P/N: Carrier: Oven:
DB-1701
30 m x 0.32 mm id, 0.25 m 123-0732 Helium at 35 cm/sec, measured at 50C 50C for 1 min 50-100C at 25/min 100-250C at 5/min Splitless, 250C, 40 sec purge activation time 1 L of 0.1 g/L in methanol FID, 300C Nitrogen makeup gas at 30 mL/min 1. 2-Nitroxylene 2. Atraton 3. Prometon 4. Propazine 5. Atrazine 6. Simazine 7. Terbuthylazine 8. Secbumeton 9. Ametryne 10. Prometryne 11. Simetryne 12. Terbutryne
DB-17
30 m x 0.25 mm id, 0.25 m 122-1732 Helium at 35 cm/sec, measured at 50C 50C for 1 min 50-100C at 25/min 100-250C at 5/min Splitless, 250C 40 sec purge activation time 1 L of 0.1 g/L in methanol FID, 300C Nitrogen makeup gas at 30 mL/min 1. 2-Nitroxylene 2. Atraton 3. Propazine 4. Prometon 5. Atrazine 6. Simazine 7. Terbuthylazine 8. Secbumeton 9. Prometryne 10. Ametryne 11. Simetryne 12. Terbutryne
Injector: Detector:
Injector:
Detector:
GC and GC/MS
C184
C183
Tetrachlorodibenzo-p-furans
Note the separation between 2,3,7,8-TCDF and 2,3,4,7-TCDF on DB-225 is also easily achievable (actually a little better!) on DB-225ms.
Column:
DB-225
30 m x 0.25 mm id, 0.25 m 122-2232 Helium at 12 mL/min 160-250C at 7/min 250C until compounds elute Splitless, 240C VG Autospec Ultima
Column:
DB-225ms
30 m x 0.25 mm id, 0.25 m 122-2932 Helium at 12 mL/min 160-250C at 7/min 250C until compounds elute Splitless, 240C VG Autospec Ultima
C500
C501
359

Environmental-Semivolatiles
Dioxins and Furans
Column:
P/N: Carrier: Oven:
Tetra Isomers
Penta Isomers
DB-Dioxin
60 m x 0.25 mm id, 0.15 m 122-2461 Helium at 34.3 cm/sec, measured at 250C 180C for 1 min 180-270C at 2.5/min 270C for 40 min
GC and GC/MS
1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. 18. 19. 20. 21. 22. 23. 24. 25. 26. 27. 28. 29. 30. 31. 32. 33.
1,3,6,8-TCDD 2,3,7,8-TCDD 1,2,8,9-TCDD 1,2,4,6,8-/1,2,4,7,9-PeCDD 1,2,3,7,8 + unknown-PeCDD 1,2,4,8,9-PeCDD 1,2,4,6,7,9-/1,2,4,6,8,9-HeCDD 1,2,3,4,7,8-HeCDD 1,2,3,4,6,9-HeCDD 1,2,3,6,7,8-HeCDD 1,2,3,7,8,9-HeCDD 1,2,3,4,6,7-HeCDD 1,2,3,4,6,7,9-HpCDD 1,2,3,4,6,7,8-HpCDD OcCDD 1,3,6,8-TCDF 2,3,7,8-TCDF 1,2,8,9-TCDF 1,3,4,6,8-PeCDF 1,2,3,4,8-PeCDF 1,2,3,7,8-PeCDF 1,2,3,4,6-PeCDF 2,3,4,7,8-PeCDF 1,2,3,8,9-PeCDF 1,3,4,6,7,9-HeCDF 1,2,3,4,7,8/1,2,4,6,8,9-HeCDF 1,2,3,6,7,8-HeCDF 2,3,4,6,7,8-HeCDF 1,2,3,7,8,9-HeCDF 1,2,3,4,8,9-HeCDF 1,2,3,4,6,7,8-HpCDF 1,2,3,4,7,8,9-HpCDF OcCDF
C041A
Hexa Isomers
Hepta Isomers
Octa Isomers
C041A
C041b
360

Aroclors 1016-1268 (without 1221)
Column:
DB-XLB
30.0 m x 0.18 mm id, 0.18 m 121-1232 Helium at 37 cm/sec, measured at 150C 100C for 1 min 100-265C at 1.2/min Hot On-column, 250C 1 L in isooctane, 12.5 ppm MSD, SIM, 340C transfer line
C549
IUPAC 3 2 1 4 10 9 7 6 5 8 19 14 30 11 18 17 12 13 27 24 16 15 32 54 34 23 29 50 26 25 31 53 28 21 33 20 51 45 22 46 73 36
Retention Time 26.03 32.69 33.92 35.61 35.74 40.70 40.85 42.15 43.11 43.64 45.84 46.58 48.01 50.08 50.25 50.60 50.85 51.69 51.74 52.08 52.95 53.46 53.70 54.87 55.33 55.54 56.14 57.51 57.60 57.98 59.42 59.43 59.79 59.84 60.05 60.10 60.36 61.48 61.87 62.44 63.60 63.97
IUPAC 69 43 52 48 49 104 39 65 47 62 38 75 44 59 42 35 71 41 96 64 40 37 72 103 68 100 67 94 57 58 102 61 98 93 76 63 88 74 70 121 95 91
Retention Time 64.03 64.08 64.57 64.85 65.24 65.62 65.80 65.85 65.86 65.87 65.96 66.23 67.36 67.95 67.98 68.52 68.89 68.93 69.31 70.13 70.22 70.33 70.34 70.74 70.90 71.75 71.99 71.99 72.03 72.78 73.18 73.22 73.69 73.83 73.97 74.03 74.33 74.68 75.15 75.40 75.45 75.55
IUPAC 66 155 55 80 84 89 56 92 60 90 101 113 150 99 152 83 119 86 112 108 145 125 97 79 116 148 78 87 136 117 115 85 111 154 110 81 120 82 151 135 77 144
Retention Time 75.65 75.83 76.58 77.19 77.48 77.52 77.69 77.78 78.45 78.64 78.78 79.37 79.43 79.47 80.10 80.57 80.64 80.87 80.89 81.13 81.15 81.18 81.48 81.87 82.12 82.63 82.78 82.85 82.94 83.33 83.48 83.54 83.89 83.96 84.44 84.67 84.93 85.61 85.89 86.21 86.47 86.69
IUPAC 147 149 139 143 140 124 107 123 109 134 106 142 188 131 118 133 184 122 114 165 146 161 132 168 153 179 105 141 176 186 137 127 130 163 164 138 160 129 178 158 175 182
Retention Time 87.25 87.53 87.82 87.83 88.20 88.29 88.69 88.81 88.93 89.02 89.04 89.27 89.54 89.62 89.71 90.17 90.67 90.73 91.05 91.17 91.26 91.66 91.86 92.22 92.28 92.93 93.48 93.90 94.08 94.75 95.11 95.16 95.41 95.76 95.77 96.35 96.50 96.58 96.87 96.99 97.74 97.78
Retention IUPAC 187 166 183 126 159 128 185 162 174 181 167 202 177 171 201 204 197 173 156 172 157 192 180 200 193 191 170 198 199 190 196 169 203 208 189 207 195 194 205 206 209
Time 98.18 98.85 99.04 99.87 100.21 100.37 100.70 101.00 101.25 101.94 101.95 102.58 102.70 103.36 103.39 103.47 103.64 105.18 105.30 105.56 105.72 106.05 106.68 106.76 107.01 107.69 110.82 110.90 111.22 111.48 112.14 112.22 112.33 115.39 116.61 116.62 116.65 120.33 121.34 124.75 128.32
GC and GC/MS
361

Congeners in DIN Method PCBs
Column:
P/N: Carrier: Oven: Injector:
DB-XLB
30 m x 0.25 mm id, 0.5 m 1 m x 0.53 mm id Retention Gap 122-1236 Helium at 34.2 cm/sec, measured at 150C 100C for 1 min 100-320C at 5.6/min Hot On-column, 250C, Split Flow 100 mL/min 2 L dilute Aroclor mixture MSD, SIM of 221.9, 255.9, 291.9, 325.8, 359.8, 395.8, 429.7, 463.7 300C transfer line
Extended Temperature Program Resolving Congeners 52 and 138

Column:
DB-XLB
30 m x 0.25 mm id, 0.5 m 1 m x 0.53 mm id Retention Gap j122-1236 Helium at 34.2 cm/sec, measured at 150C 100C for 1 min 100-275C at 1.6/min Hot On-column, 250C, Split Flow 100 mL/min 2 L dilute Aroclor mixture MSD, SIM of 221.9, 255.9, 291.9, 325.8, 359.8, 395.8, 429.7, 463.7 300C transfer line
Detector:
Detector:
GC and GC/MS
C370
C371
362

PCBs by EPA Method 8082
Column:
P/N: 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. 18. 19. 20. 21. IUPAC 1 Tetrachloro-m-xylene (IS/SS) IUPAC 5 IUPAC 18 IUPAC 31 IUPAC 52 IUPAC 44 IUPAC 66 IUPAC 101 IUPAC 87 IUPAC 110 IUPAC 151 IUPAC 153 IUPAC 141 IUPAC 137 IUPAC 187 IUPAC 183 IUPAC 180 IUPAC 170 IUPAC 206 Decachlorobiphenyl (IS/SS)
DB-35ms
30m x 0.32 mm id, 0.25 m 123-3832
Column:
P/N: Carrier: Oven:
DB-XLB
30 m x 0.32 mm id, 0.50 m 123-1236 Helium at 45 cm/sec (EPC in constant flow mode) 110C for 0.5 min 110-320C at 15C/min 320C for 5 min Splitless, 250C 30 sec purge activation time 50 pg per component ECD, 350C Nitrogen makeup gas (column + makeup flow = 30 mL/min constant flow)
Injector:
Detector:
14 15 18
19
DB-35ms
11 10 12
17 16
20
8 9 5 6 3 4 7
13
22
GC and GC/MS
C790
10 Time (min)
12
14
16
DB-XLB
15 14 17 16 13 9
19 18
10 11 12 8 7 5 6 3 4
20
21
6
C791
10 Time (min)
12
14
16
363

Chlordane
Column:
P/N: Carrier: Oven:
Toxaphene
Column:
P/N: Carrier: Oven:
DB-35ms
30m x 0.32 mm id, 0.25 m film thickness 123-3832 Helium at 45 cm/sec (EPC in constant flow mode) 110C for 0.5 min 110-320C at 15C/min 320C for 5 min Splitless, 250C 30 sec purge activation time 1 g/mL per component in Methanol ECD, 350C Nitrogen makeup gas (column + makeup flow = 30 mL/min constant flow)
DB-35ms
30m x 0.32 mm id, 0.25 m film thickness 123-3832 Helium at 45 cm/sec (EPC in constant flow mode) 110C for 0.5 min 110-320C at 15C/min 320C for 5 min Splitless, 250C 30 sec purge activation time 1 g/mL per component in Methanol ECD, 350C Nitrogen makeup gas (column + makeup flow = 30 mL/min constant flow)
Injector:
Injector:
Detector:
Detector:
DB-35ms
DB-35ms
GC and GC/MS
4
C1083
10 Time (min)
12
14
16
4
C1085
10 12 Time (min)
14
16
18
Pyrethrins
Chromatogram courtesy of Khan Nguyen and Richard Moorman of Sandoz Agro Inc.
Column:
P/N: Carrier: Oven:
DB-1
30 m x 0.32 mm id, 0.25 m 123-1032 Helium at 39 cm/sec, measured at 150C 180C for 11 min 180-200C at 10/min 200C for 8 min 200-210C at 10/min 210C for 18 min 210-245C at 30/min 245C for 4 min Injector: Split 1:20,1 L, 250C Detector: FID, 300C Helium makeup gas at 30 mL/min 1. Heptadecane 2. Octadecane 3. Endo-MGK 264 4. Exo-MGK 264 5. Methoprene 6. Cinerin I 7. Jasmolin I 8. Pyrethrin I 9. BPE (PB) 10. Cinerin II 11. Jasmolin II 12. Pyrethrin II MGK 264 = N-Octylbicycloheptene dicarboximide BPE = Butylcarbityl 6-propylpiperonyl ether
C525
364

Organotin Compounds
Column:
P/N: Carrier: Oven: Injection: Detector:
Organomercury Compounds
Column:
1. 2. 3. 4. 5. 6. 7. Dimethylpentyltin Tetrabutyltin Methtyltripentyltin Tributylpentyltin Dibutylpentyltin Butlypentyltin Tetrapentyltin P/N: Carrier: Oven: Injection: Detector:
HP-1
25 m x 0.32 mm x 0.17 m 19091Z-012 Helium, 100 kPa 50C for 1 min 50-260C at 15C/min Splitless, 1 L AED 330C
HP-5
25 m x 0.2 mm id, 0.11 m 19091J-002 Helium, 0.75 mL/min constant flow 80C for 4 min 80-300C at 5C/min Splitless, inlet 300C, 1 L AED 300C, Tin selective at 303.4 mm
Sn (303 nm)
5 2 3 4 6 7
MeHgPh 40 pg
Ph2Hg 38 pg
7
C1031
9 Time (min)
10
11
12
20
C1029
25
30 Time (min)
35
40
45
GC and GC/MS
Organotin Compounds
Column:
HP-5
25 m x 0.2 mm id, 0.11m 19091J-002 Helium, 0.75 mL/min constant flow 60-360C at 5C/min Splitless, 1 L, inlet 300C AED 300C, Hg selective at 254 mm
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Oct2SnPh
Consult Agilent's online, searchable Chromatogram Database

Containing hundreds of commonly analyzed compounds, this resource helps chromatographers select the right analytical technique, column and conditions for an application. Search the extensive database for GC, LC and CE applications by compound name or CAS number.
Standard Solution
Bu4Sn Bu2SnPh Bu2SnPh2 BuSnPh2
Solvent Vent
10
20
30 Time (min)
40
50
Harbor Sediment
Me2SnPh 10 pg
BuSnPh2 35 pg Bu4Sn 14 pg Bu SnPh 2 2 17 pg Bu2 SnPh2 78 pg Ph4Sn Oct SnPh 2 35 pg
Solvent Vent 0 C1032 10 20 30 Time (min)
To access the Chromatogram Database go to www.agilent.com/chem and click on "Library."

50
40
365

USEPA Method 8270 Semivolatile Compounds
Column:
P/N: Carrier: 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. 18. 19. 20. 21. 22. 23. 24. 25. 26. 27. 28. n-Nitrosodimethylamine Pyridine 2-Fluorophenol Phenol-d5 Phenol Aniline bis(2-Chloroethyl) ether 2-Chlorophenol 1,3-Dichlorobenzene 1,4-Dichlorobenzene-d4 1,4-Dichlorobenzene Benzyl alcohol 1,2-Dichlorobenzene 2-Methylphenol bis(2-Chloroisopropyl) ether 4-Methylphenol n-Nitroso-di-n-propylamine Hexachloroethane Nitrobenzene-d5 Nitrobenzene Isophorone 2-Nitrophenol 2,4-Dimethylphenol Benzoic acid bis(2-Chloroethoxy) methane 2,4-Dichlorophenol 1,2,4-Trichlorobenzene Naphthalene-d8 29. 30. 31. 32. 33. 34. 35. 36. 37. 38. 39. 40. 41. 42. 43. 44. 45. 46. 47. 48. 49. 50. 51. 52. 53. 54. 55. 56. Naphthalene 4-Chloroaniline Hexachlorobutadiene 4-Chloro-3-methylphenol 2-Methylnaphthalene Hexachlorocyclopentadiene 2,4,6-Trichlorophenol 2,4,5-Trichlorophenol 2-Fluorobiphenyl 2-Chloronaphthalene 2-Nitroaniline Dimethylphthalate 2,6-Dinitrotoluene Acenaphthylene 3-Nitroaniline Acenaphthene-d10 Acenaphthene 2,4-Dinitrophenol 4-Nitrophenol Dibenzofuran 2,4-Dinitrotoluene Diethylphthalate 4-Chlorophenyl-phenyl ether Fluorene 4-Nitroaniline 4,6-Dinitro-2-methylphenol n-Nitrosodiphenylamine Azobenzene 57. 58. 59. 60. 61. 62. 63. 64. 65. 66. 67. 68. 69. 70. 71. 72. 73. 74. 75. 76. 77. 78. 79. 80. 81. 82. 83. 2,4,6-Tribromophenol 4-Bromophenyl-phenylether Hexachlorobenzene Pentachlorophenol Phenanthrene-d10 Phenanthrene Anthracene Carbazole Di-n-butylphthalate Fluoranthene Pyrene Terphenyl-d14 Benzidine Butylbenzylphthalate 3,3'-Dichlorobenzidine Benzo[a]anthracene Chrysene-d12 Chrysene bis(2-Ethylhexyl)phthalate Di-n-octylphthalate Benzo[b]fluoranthene Benzo[k]fluoranthene Benzo[a]pyrene Perylene-d12 Indeno[1,2,3-cd]pyrene Dibenz[a,h]anthracene Benzo[g,h,I]perylene
HP-5ms
30 m x 0.25 mm id, 0.5 um 19091S-133 Ramped flow 1.2 mL/min for 0.0 min ramp at 99 mL/min to 2.0 mL/min 2.0 mL/min for 0.35 min ramp at 10 mL/min to 1.2 mL/min 40C for 1.0 min 40-100C at 15C/min 100-240C at 20C/min 240-310C at 10C/min Splitless, 250C 30 mL/min purge flow at 0.35 min 1 uL of 50 ng standard 5973 MSD, 310C transfer line Scan range 35-500 amu, 3.25 scans/sec
Oven:
Inlet: Sample: Detector:
GC and GC/MS
75 45 37 22,23 29 33 38 32 16 4,5 21 28 35 36 42 40 44 41
43
52 48 51 56 55 50
62 63 64
65
66
67 70 68,69 74 76
58 60 72 73
49
53 54 59 60 71 77
78
3 1,2
12 26 30 14 13 25 27 31 11 20 10 19 9 17 8 15 6 18 7
39
47
46
79 57 80 82 81 83
34
24
10
12
14 Time (min)
16
18
20
22
24
C1080
366

EPA Method 525.2
Column: DB-5ms
P/N: Carrier: 30 m x 0.25 mm id, 0.25 m 122-5532 Helium, at 32 cm/sec, measured at 45C, constant flow mode 45C for 1 min 45-130C at 30/min 130C for 3 min 130-180C at 12/min 180-240C at 7/min 240-325C at 12/min 325C for 5 min Splitless 1.0 min purge activation time, 300C FocusLiner MSD, 325C transfer line full scan at m/z 45-450 Composite mixture of Accustandard Method 525.2 standards (M-525.2-SV-ASL, M-525.2-FS-ASL, M-525.2-CP-ASL, M-525.2-NP1-ASL, M-525.2-NP2-ASL): target compounds at 2 ng/L, IS/SS at 5 ng/L Peak No./Compound 28. 2,3-Dichlorobiphenyl 29. Hexachlorobenzene 30. Gesatamine (aka Atraton) 31. Prometon 32. Atrazine 33. Simazine 34. -BHC 35. Pentachlorophenol 36. Propazine 37. -BHC 38. Terbufos 39. Pronamide 40. Diazinon 41. Phenanthrene-d10 (IS #2) 42. Chlorothalonil 43. Phenanthrene 44. Terbacil 45. Methyl paraoxon 46. Disulfoton 47. Anthracene 48. -BHC 49. 2,4,5-Trichlorobiphenyl 50. Metribuzin 51. Alachlor 52. Simetryn 53. Ametryn 54. Heptachlor 55. Prometryne 56. Prebane (aka Terbutryne) 57. Bromacil 58. Di-n-butyl phthalate 59 2,2',4,4'-Tetrachlorobiphenyl 60. Metolachlor 61. Dursban (aka Chlorpyrifos) 62. Cyanazine 63. Dacthal (aka DCPA methyl ester) 64. Aldrin 65. Triadimefon 66. Diphenamid 67. MGK-264 (isomer A) 68. MGK-264 (isomer B) 69. Heptachlor epoxide 70. 2,2',3',4,6-Pentachlorobiphenyl 71. Merphos 72. -Chlordane 73. Tetrachlorvinphos (aka Stirifos) RT 13.74 13.77 13.99 14.14 14.26 14.27 14.28 14.35 14.35 14.52 14.62 14.69 14.76 14.85 14.89 14.92 15.02 15.04 15.05 15.06 15.20 15.59 15.95 16.14 16.23 16.33 16.36 16.40 16.72 16.79 16.90 17.02 17.11 17.15 17.23 17.27 17.29 17.43 17.73 17.78 18.11 18.28 18.34 18.36 18.88 18.95 m/z 222/152 284 196/169 225/168 200/215 201/186 181 266 214/172 181 57 173 137/179 188 266 178 161 109 88 178 181 256 198 160 213 227/170 100 241/184 226/185 205 149 292 162 197/97 225/68 301 66 57 72/167 164/66 164 81 326 209/153 373 109 Peak No./Compound 74. Butachlor 75. Pyrene-d10 (SS) 76. Pyrene 77. -Chlordane 78. Endosulfan I 79. trans-Nonachlor 80. Fenamiphos 81. Napropamide 82. Tricyclazole 83. p,p-DDE 84. DEF 85. 2,2',4,4',5,6'-Hexachlorobiphenyl 86. Dieldrin 87. Carboxin 88. Endrin 89. Chlorobenzilate 90. Endosulfan II 91. p,p-DDD 92. Endrin aldehyde 93. Norflurazon 94. Benzyl butyl phthalate 95. Endosulfan sulfate 96. p,p-DDT 97. Hexazinone 98. bis (2-Ethylhexyl) adipate 99. Triphenylphosphate (SS) 100. Endrin ketone (breakdown product) 101. 2,2',3,3',4,4',6-Heptachlorobiphenyl 102. Benz[a]anthracene 101. Chrysene-d12 (IS #3) 104. 2,2',3,3',4,5',6,6'-Octachlorobiphenyl 105. Methoxychlor 106. Chrysene 107. bis(2-Ethylhexyl) phthalate 108. Fenarimol 109. cis-Permethrin 110. trans-Permethrin 111. Benzo[b]fluoranthene 112. Benzo[k]fluoranthene 113. Fluridone 114. Benzo[a]pyrene 115. Perylene-d12 (SS) 116. Indeno[1,2,3-c,d]pyrene 117. Dibenz[a,h]anthracene 118. Benzo[g,h,i]perylene RT 19.03 19.13 19.18 19.21 19.22 19.28 19.33 19.39 19.61 19.76 19.84 19.90 19.92 19.97 20.43 20.56 20.68 20.77 21.01 21.36 21.49 21.53 21.61 21.68 21.87 21.98 22.52 22.59 22.66 22.68 22.70 22.73 22.74 23.10 23.80 24.38 24.50 25.06 25.12 25.66 25.67 25.78 27.63 27.69 28.11 m/z 176/160 212 202 375/373 195 409 303/154 72 189 246 57/169 360 79 143 67/81 139 195 235/165 67 145 149 272 235/165 171 129 326/325 67/317 394/396 228 240 430/428 227 228 149 139 183 183 252 252 328 252 264 276 278 276
Oven:
Injector: Detector: Sample:
Peak No./Compound 1. Isophorone 2. 1,3-Dimethyl-2nitrobenzene (SS) 3. Dichlorvos 4. Hexachlorocyclo-pentadiene 5. EPTC 6. Mevinphos 7. Butylate 8. Vernolate 9. Dimethyl phthalate 10. Terrazole (aka Etridazole) 11. 2,6-Dinitrotoluene 12. Tillam (aka Pebulate) 13. Acenaphthylene 14. Acenaphthene-d10 (IS #1) 15. Chloroneb 16. 2-Chlorobiphenyl 17. Tebuthiuron 18. 2,4-Dinitrotoluene 19. Molinate 20. Diethyl phthalate 21. Fluorene 22. Propachlor 23. Ethoprop 24. Cycloate 25. Chlorpropham 26. Trifluralin 27. -BHC
RT 5.85 6.65 7.41 8.87 9.17 10.09 10.18 10.42 10.45 10.47 10.56 10.61 10.65 11.00 11.17 11.19 11.37 11.51 11.68 12.21 12.35 12.46 12.82 12.86 13.08 13.14 13.69
m/z 82 134 109 237 128 127 57/146 128 163 211/183 165 128 152 164 191 188 156 165 126 149 166 120 158 83/154 127 306 181
GC and GC/MS
C741
367

EPA Method 8061 (Phthalate Esters)
Column:
P/N: Carrier: 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. Dimethyl phthalate Diethyl phthalate Benzyl benzoate (IS) Diisobutyl phthalate Di-n-butyl phthalate bis(4-Methoxyethyl) phthalate bis(4-Methyl-2-pentyl) phthalate *Two isomers bis(2-Ethoxyethyl) phthalate Diamyl phthalate Dihexyl phthalate Butyl benzyl phthalate 12. 13. 14. 15. 16. 17. 18. 19. 20. Hexyl 2-ethylhexyl phthalate bis(2-n-Butoxyethyl) phthalate Dicyclohexyl phthalate bis(2-Ethylhexyl) phthalate Diphenyl phthalate (SS Diphenyl isophthalate (SS) Di-n-octyl phthalate Dibenzyl phthalate (SS) Dinonyl phthalate
DB-5ms
20 m x 0.18 mm id, 0.18 m 121-5522 Helium at 49 cm/sec, measured at 80C constant flow program 80C for 0.5 min 80-160C at 30/min 160-320C at 15/min Splitless, 300C 30 sec purge activation time 1 L of 20 ng/L Method 8061 mixture (Accustandard) in hexane MSD, m/z 50-400 full scan 325C transfer line
Oven:
Injector:
* Two isomers SS - Surrogate Standard
Detector:
GC and GC/MS
C730
PAHs
Column:
P/N: Guard Column: P/N: Carrier: Oven:
DB-17ms
30 m x 0.25 mm id, 0.25 m 122-4732 1 m x 0.53 mm id 160-2535 Helium at: 34.1 cm/sec, measured at 150C 95C for 0.5 min 95-340C at 5/min 340C for 5 min Split 1:40, 300C 2 L, PAH standard MSD, 340C transfer line Scan 80-330 amu
Injector: Detector:
8. 9. 10. 11. 12. 13. 14. 15. 16. 17. 18. 19. 20. 21. 22. 23. 24. 25. 26. 27. 28. 29. 30. 31. 32. 33.
Name 1. Naphthalene 2. 2-Methylnaphthalene 3. 1-Methylnaphthalene 4. Azulene 5. Acenaphthene 6. Biphenyl 7. 2,6-Dimethylnaphthalene
Ions 128 142,141 142,141 128 154 154 156,155
Acenaphthalene Dibenzofuran Dibenzo-p-dioxin Fluorene 1-Nitronaphthalene 9,10-Dihydroanthracene 2-Nitronaphthalene 2-Nitrobiphenyl Dibenzothiophene Phenanthrene Anthracene 3-Nitrobiphenyl 4-Nitrobiphenyl 5,6-Benzoquinoline Carbazole 2-Methylanthracene 1,2,3,4-Tetrahydrofluoranthene 2-Phenylnaphthalene 9-Methylanthracene 3,6-Dimethylphenanthrene 1,3-Dinitronaphthalene 1,5-Dinitronaphthalene Fluoranthene 2,2'-Dinitrobiphenyl Pyrene 2-Methylfluoranthene
152 168,139 184 166,165 127,173 179,180 127,173 152,115 184 178 178 199,152 199,152 179 167 192,191 178,206 204 192,191 206,191 126,218 218,114 202 198,139 202 216,215
34. 2,3-Benzofluorene 35. Dodecahydrotriphenylene 36. 1-Amino-4nitronaphthalene 37. 9-Phenylanthracene 38. 1,2-Benzanthracene 39. Chrysene 40. Benz[a]anthracene7,12-dione 41. 2,7-Dinitrofluorene 42. Benzo[b]fluoroanthene 43. Benzo[k]fluoranthene 44. 7,12-Dimethylbenz [a]anthracene 45. Benzo[e]pyrene 46. Benzo[a]pyrene 47. Perylene 48. 3-Methylcholanthrene 49. 9,10-Diphenylanthracene 50. 1,2,3,4-Dibenzanthracene 51. 1,2,5,6-Dibenzanthracene 52. Benzo[g,h,i]perylene
216,215 240,198 188,115 254,253 228 240 258,202 256,163 252 252 256,241 252 252 252 268 330 278 278 276
C467A
368

Phenols
Column:
P/N: 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. 18. 19. 20. 21. Phenol 2-Chlorophenol 2-Methylphenol 4-Methylphenol 3-Methylphenol 2-Chloro-5-methylphenol 2,6-Dimethylphenol 2-Nitrophenol 2,4-Dimethylphenol 2,5-Dimethylphenol 2,4-Dichlorophenol 2,3-Dimethylphenol 2,5-Dichlorophenol 2,3-Dichlorophenol 2-Chlorophenol 4-Chlorophenol 3,4-Dimethylphenol 2,6-Dichlorophenol 4-Chloro-2-methylphenol 4-Chloro-3-methylphenol 2,3,5-Trichlorophenol 22. 23. 24. 25. 26. 27. 28. 29. 30. 31. 32. 33. 34. 35. 36. 37. 38. 39. 40. 2,4-Dibromophenol 2,4,6-Trichlorophenol 2,4,5-Trichlorophenol 2,3,4-Trichlorophenol 3,5-Dichlorophenol 2,3,6-Trichlorophenol 3,4,-Dichlorophenol 3-Nitrophenol 2,5-Dinitrophenol 2,4-Dinitrophenol 4-Nitrophenol 2,3,5,6-Tetrachlorophenol 2,3,4,5-Tetrachlorophenol 2,3,4,6-Tetrachlorophenol 3,4,5-Trichlorophenol 2-Methyl-4,6-dinitrophenol Pentachlorophenol Dinoseb 2-Cyclohexyl-4,6-dinitrophenol
DB-5ms
30 m x 0.25 mm id, 0.25 m 122-5532
Column:
P/N: Carrier: Oven:
DB-XLB
30 m x 0.25 mm id, 0.25 m 122-1232 He at 1.2 mL/min Constant Flow 40C for 2.00 min 40-100C at 40C/ min 100C for 0.50 min 100-140C at 2C/min 140-340C at 30C/min Pulsed Splitless, 200C Pulse Pressure & Time: 25.0 psi for 1.00 min Purge Flow & Time: 50.0 mL/min for 0.25 min Gas Saver Flow & Time: 20.0 mL/min for 3.00 min MSD Transfer Line Temp at 320C Quadrapole at 150C 12, 13 Source at 230C
Injector:
Detector:
DB-5ms
15, 17 22, 23
36, 37
24 26 2728 4, 5 6 1 2 3 7 9 10 14 18 11 19 20 21 25 32 29, 30
34 35 33 38 39 40
GC and GC/MS
31
5 C1081
9 Time (min)
10
11
12
36
DB-XLB
38 39 12 7 6
8,10
11,13 17 30,32 14 22 19 16 18 20 29 21 23,24 25 26 27 28
34
35 33 37
40
2 1
3 5 4 9
15
31
6 C1082
10
12
14
16
18 Time (min)
20
22
24
26
28
30
369

EPA 552.2
Column:
P/N:
DB-35ms
30m x 0.32 mm id, 0.25 m film thickness 123-3832 30 m x 0.32 mm id, 0.50 m film thickness 123-1236 Helium at 45 cm/sec (EPC in constant flow mode) 40C for 0.5 min 40-200C at 15C/min 200C for 2 min Splitless, 250C 30 sec purge activation time 50 pg per component ECD, 350C Nitrogen makeup gas (column + makeup flow = 30 mL/min constant flow)
11
DB-XLB
P/N: Carrier: Oven:
Injector:
1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12.
Chloroacetic acid Bromoacetic acid Dichloroacetic acid Dalapon Trichloroacetic acid 1,2,3-Trichloropropane (IS) Bromochloroacetic acid Bromodichloroacetic acid Dibromoacetic acid 2,3-Dibromopropionic acid (SS) Chlorodibromoacetic Tribromoacetic acid
12
Detector:
DB-35ms
9 7
2, 3
4 6
min
C788
GC and GC/MS
DB-XLB
12 11
8, 9 7
3 5 2 4 1 6 10
min
C788
370

Environmental-Volatiles
EPA Volatiles by GC/MS (Split Injector)
Column:
P/N: Carrier: Oven: 9. 10. 11. 12. 13. 14. 15. 16. 17. 18. 19. 20. 21. 22. 23. 24. Acetone Iodomethane Carbon disulfide Allyl chloride Methylene chloride Acrylonitrile Methyl-tert-butyl ether trans-1,2-Dichloroethene Hexane 1,1-Dichloroethane 2-Butanone cis-1,2-Dichloroethene 2,2-Dichloropropane Propionitrile Methyl acrylate Methacrylonitrile 25. 26. 27. 28. 29. Bromochloromethane Tetrahydrofuran Chloroform Pentafluorobenzene (IS) 1,1,1-Trichloroethane 30. 31. 32. 33. 34. 35. 36. 37. 38. 39. 40. 1-Chlorobutane 1,1-Dichloropropene Carbon tetrachloride Benzene 1,2-Dichloroethane 2,2-Dimethylhexane Fluorobenzene (IS) 1,4-Difluorobenzene (IS) Trichloroethene 1,2-Dichloropropane Methyl methacrylate
DB-VRX
60 m x 0.25 mm id, 1.4 m 122-1564 Helium at 30 cm/sec, measured at 45C 45C for 10 min 45-190C at 12/min 190C for 2 min 190-225C at 6/min 225C for 1 min Purge and trap (O.I.A. 4560) 40 ppb per each component in 5 mL water Helium for 11 min at 40 mL/min Tenax/Silica Gel/Carbosieve 0.6 min at 220C Split injector at 110C with split flow set at 30 mL/min MSD, 235C transfer line full scan 35-260 amu (m/z 44 subtracted)
61,62
68,63
Injector: Sample: Purge: Trap: Desorb: Interface: Detector:
60 64 48 57 58
70 71 66 72 73 67 75
Compound list for both chromatograms (NOTE: Some compounds not present in both chromatograms) 1. Dichlorodifluoromethane 2. Chloromethane 3. Vinyl chloride 4. Bromomethane 5. Chloroethane 6. Trichlorofluoromethane 7. Diethyl ether 8. 1,1-Dichloroethene
10,14 13 12 2 3 4 1 7 5 6 9 16 15 11 18,22 19 20 27 24 25 21
74 79 78
53 33 29,30 44 34 28 23 26 31 32 41 36 37 38 42,43 47,45 49 39 40 46
50 69
76
80 83 81 82 84
51 52 54 55 56
59 77
89 85 88 90 91 86
65
GC and GC/MS
87
10
15 Time (min)
20
25
30
C378B
Column:
P/N: Carrier: Oven:
DB-624
60 m x 0.25 mm id, 1.4 m 122-1364 Helium at 31 cm/sec, measured at 40C 40C for 3 min 40-90C at 8/min 90C for 4 min 90-200C at 6/min 200C for 5 min Purge and trap (O.I.A. 4560) 40 ppb per each component in 5 mL water Helium for 11 min at 40 mL/min Tenax/Silica Gel/Carbosieve 0.6 min at 220C Split injector at 110C with split flow set at 30 mL/min MSD, 235C transfer line, full scan 35-260 amu (m/z 44 subtracted)
33,34
Injector: Sample: Purge: Trap: Desorb: Interface: Detector:
53. 54. 55. 56. 57. 58. 59. 60. 61. 62. 63. 64. 65. 66. 67. 68. 69.
1,3-Dichloropropane 2-Hexanone Dibromochloromethane 1,2-Dibromoethane 1-Chloro-3-fluorobenzene (IS) Chlorobenzene 1,1,1,2-Tetrachloroethane Ethylbenzene m-Xylene p-Xylene o-Xylene Styrene Bromoform Isopropylbenzene 4-Bromofluorobenzene (SS) 1,1,2,2-Tetrachloroethane Bromobenzene
48
70. 71. 72. 73. 74.

61,62
1,2,3-Trichloropropane trans-1,4-Dichloro-2-butene n-Propylbenzene 2-Chlorotoluene 1,3,5-Trimethylbenzene
63,64
75. 76. 77. 78. 79. 80. 81. 82. 83. 84. 85. 86. 87. 88. 89. 90. 91.
4-Chlorotoluene tert-Butylbenzene Pentachloroethane 1,2,4-Trimethylbenzene sec-Butylbenzene 1,3-Dichlorobenzene p-Isopropyltoluene 1,4-Dichlorobenzene n-Butylbenzene 1,2-Dichlorobenzene Hexachloroethane 1,2-Dibromo-3-chloropropane Nitrobenzene 1,2,4-Trichlorobenzene Hexachlorobutadiene Naphthalene 1,2,3-Trichlorobenzene
72 52,53 60 66 73 74 70,71 67 75
79 80 78 76 81 82 84 90 85 88 91 83
Compound list continued 41. Dibromomethane 42. Bromodichloromethane 43. 2-Nitropropane 44. Chloroacetonitrile 45. cis-1,3-Dichloropropene 46. 4-Methyl-2-pentanone 47. 1,1-Dichloro-2-propanone 48. Toluene 49. trans-1,3-Dichloropropene 50. Ethyl methacrylate 51. 1,1,2-Trichloroethane 52. Tetrachloroethene
4 1 23 5 6
15,16 14 11 10 18 8,9 12 7 17 35 43,44 47 13 24 23 19,20,21,22 31,32 26,27 38 25 29 36 39 41 42 46 54 30 40 45 50 49 51 55 56 59
58 69 68
65
89
86
87
10
15 Time (min)
20
25
30
35
C378A
371

High Speed VOC EPA 8260
Column:
P/N: Carrier: Oven: 32. 33. 34. 35. 36. 37. 38. 39. 40. 41. 42. 43. 44. 45. 46. 47. 48. 49. 50. 51. 52. 53. 54. 55. 56. 57. 58. 59. 60. 61. 62. 63. 64. 65. 66. 67. 68. 69. 70. 71. 72. 73. 74. 2,2-Dichloropropane Ethyl acetate Ethyl-tert-butyl ether Methyl acrylate Dibromofluoromethane IS Isobutanol Dichloroethane-d4 IS Pentafluorobenzene 1,2-Dichloroethane 1,1,1-Trichloroethane 1-Chlorobutane Crotonaldehyde 2-Chloroethanol 1,1-Dichloropropene 1-Butanol Carbon tetrachloride Chloroacetonitrile Benzene tert-Amylmethyl ether Fluorobenzene IS 2-Pentanone Dibromomethane 1,2-Dichloropropane Trichloroethene Bromodichloromethane 2-Nitropropane 1,4-Dioxane Epichlorohydrin Methyl methacrylate cis-1,3-Dichloropropene Propiolactone Bromoacetone Pyridine trans-1,3-Dichloropropene 1,1,2-Trichloroethane Toluene-d8 IS Toluene 1,3-Dichloropropane Paraldehyde Ethyl methacrylate Dibromochloromethane 3-Chloropropionitrile 1,2-Dibromoethane 41,42 39,40 46 27 26 2.0 3.0 37 38 43,44 50 52 58 4.0 62 45 47 48 51 53 75. Tetrachloroethene 76. 1,1,1,2-Tetrachloroethane 77. 1-Chlorohexane 78. Chlorobenzene 79. Ethylbenzene 80. Bromoform 81. m-Xylene 82. p-Xylene 83. trans-Dichlorobutene 84. 1,3-Dichloro-2-propanol 85. Styrene 86. 1,1,2,2-Tetrachloroethane 87. o-Xylene 88. 1,2,3-Trichlropropane 89. cis-Dichlorobutene 90. 4-Bromofluorobenzene IS 91. Isopropylbenzene 92. Bromobenzene 93. Propylbenzene 94. 2-Chlorotoluene 95. 4-Chlorotoluene 96. 1,3,5-Trimethylbenzene 97. Pentachloroethane 98. tert-Butylbenzene 99. 1,2,4-Trimethylbenzene 100. sec-Butylbenzene 101. 1,3-Dichlorobenzene 102. Benzylchloride 103. 1,4-Dichlorobenzene-d4 IS 104. 1,4-Dichlorobenzene 105. Isopropyltoluene 106. 1,2-Dichlorobenzene 107. Butylbenzene 108. 1,2-Dibromo-3-chloropropane 109. Hexachloroethane 110. Nitrobenzene 111. 1,2,4-Trichlorobenzene 112. Naphthalene 113. Hexachlorobutadiene 114. 1,2,3-Trichlorobenzene 55 54 56,57 59,60 61
DB-VRX
20 m x 0.18 mm id, 1.0 m film 121-1524 Helium at 55 cm/sec (1.5 mL/min) 45C for 3.0 minutes 45-190C at 36C/min 190-225C at 20C/min 225C for 0.5 min Tekmar 3100 Purge and Trap Vocarb 3000 5 mL 11 minutes 245C 1 minute at 250C 10 minutes at 260C 100C
Interface: Agilent 5973 MSD Scan range: Scan rate: Quad temperature: Source temperature: Transfer line temp:
Split injector at 150C, 60:1 split ratio 35-260 amu 3.25 scans/sec 150C 200C 200C
Injector: Trap: Sample volume: Purge: Desorb preheat: Desorb: Bake: Line and valve temp: 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. 18. 19. 20. 21. 22. 23. 24. 25. 26. 27. 28. 29. 30. 31.
Sample Concentration Halogentated and aromatic analytes at 40 ppb Internal standards at 20 ppb Polar analytes (i.e., ethers, alcohols and ketones at 100-800 ppb) 28,29
GC and GC/MS
Dichlorodifluoromethane Chloromethane Hydroxypropionitrile Vinyl chloride Bromomethane Chloroethane Ethanol Acetonitrile Acrolein Trichlorofluoromethane Isopropyl alcohol Acetone Ethyl ether 1,1-Dichloroethene tert-Butyl alcohol Acrylonitrile Methylene chloride Allyl chloride Allyl alcohol 1-Propanol Propargyl alcohol trans-1,2-Dichloroethene MTBE 1,1-Dichloroethane Propionitrile 2-Butanone Diisopropyl ether cis-1,2-Dichloroethene Methacrylonitrile Bromochloromethane Chloroform
Ideal for 5973 MSD
8-10
16
14,15 17 18
22
49
23
2 1 3,4
5 13 6 7 11 12 19 1.0 21 20 25 24 32-34 30 31
35,36
C797a
Time (min)
98 80-82 96,97 77,78 99
101,102 105 100

103,104
86,87
90,91 93 107 98 95 94 92 109 106
79 68
84,85
6.4
6.5
6.6
111
114
113 112
75 76 65 63, 64 66 67 69,70 71 88 72 74 73 83 89 108 110
C797b
5.0
6.0
7.0
8.0
Time (min)
372

Extended Analyte List for EPA Method 8021 DB-624/VRX Dual Column
Columns: DB-624
P/N: 75 m x 0.45 mm id, 2.55 m 124-1374 10. 11. 12. 13. 14. 15. 16. 17. 18. 19. 20. 21. 22. 23. 24. 25. 26. 27. 28. 29. 30. 31. 32. 33. 34. 35. 36. 37. 38. 39. 40. Allyl chloride Methylene chloride trans-1,2-Dichloroethene 1,1-Dichloroethane Chloroprene cis-1,2-Dichloroethene 2,2-Dichloropropane Bromochloromethane Chloroform 1,1,1-Trichloroethane Carbon tetrachloride 1,1-Dichloropropene Benzene 1,2-Dichloroethane Fluorobenzene (IS) Trichloroethene 1,2-Dichloropropane Dibromomethane Trifluorotoluene (IS) Bromodichloromethane 2-Chloroethyl vinyl ether cis-1,3-Dichloropropene Toluene trans-1,3-Dichloropropene 1,1,2-Trichloroethane Tetrachloroethene 1,3-Dichloropropane Dibromochloromethane 1,2-Dibromoethane 1-Chloro-3-fluorobenzene (IS) Chlorobenzene 41. 42. 43. 44. 45. 46. 47. 48. 49. 50. 51. 52. 53. 54. 55. 56. 57. 58. 59. 60. 61. 62. 63. 64. 65. 66. 67. 68. 69. 70. 71. 1,1,1,2-Tetrachloroethane Ethylbenzene m-xlyene p-Xylene Styrene o-Xylene Bromoform Isopropylbenzene cis-1,4-Dichlorobutene 1,1,2,2-Tetrachloroethane Bromobenzene 1,2,3-Trichloropropane n-Propylbenzene 2-Chlorotoluene 1,3,5-Trimethylbenzene 4-Chlorotoluene tert-Butylbenzene 1,2,4-Trimethylbenzene sec-Butylbenzene 1,3-Dichlorobenzene p-Isopropyltoluene 1,4-Dichlorobenzene Benzyl chloride n-Butylbenzene 1,2-Dichlorobenzene bis (2-Chloroisopropyl) ether 1,2-Dibromo-3-chloropropane 1,2,4-Trichlorobenzene Hexachlorobutadiene Naphthalene 1,2,3-Trichlorobenzene
DB-VRX
75 m x 0.45 mm id, 2.55 m Guard column: 5 m x 0.53 mm id deactivated fused silica 3-way universal glass union P/N: 124-1574 Carrier: Helium at 9 mL/min, measured at 35C Oven: 35C for 12 min 35-60C at 5/min 60C for 1 min 60-200C at 17/min 200C for 5 min Injector: Purge and trap (O.I.A. 4560) J&W LVI (Low Volume Injector), 150C 20 ppb per component in 5 mL water Trap: VOCARB 3000 Preheat: 175C Desorb: 260C for 1 min Detector A: PID (O.I.A. 4430), 200C Helium makeup gas at 20 mL/min Detector B: ELCD (O.I.A. 4420), with NiCat reaction tube in the halogen mode, 950C reactor temperature
Compound list for all chromatograms 1. Dichlorodifluoromethane 2. Chloromethane 3. Vinyl chloride 4. Bromomethane 5. Chloroethane 6. Trichlorofluoromethane 7. 2-Chloropropane (IS) 8. 1,1-Dichloroethene 9. Iodomethane
GC and GC/MS
Detector B: ELCD
DB-624
DB-VRX
C432
373

Detector A: PID
DB-624
GC and GC/MS
DB-VRX
374

Fast VOA Analysis
Columns: DB-624
P/N: Carrier: Oven: 20 m x 0.18 mm id, 1.0 m 121-1324 Helium at 37 cm/sec, (constant flow mode) 35C for 4 min 35-200C at 15/min 200C for 0.1 min 60-200C at 17/min Purge and trap (Tekmar LSC 3000) 10 ppb per component in 25 mL water Helium for 11 min at 50 mL/min 250C 260C for 2 min 100C 5973 MSD, 250C transfer line Full scan 35 -260 amu 3.25 scans per second 15. 16. 17. 18. 19. 20. 21. 22. 23. 24. 25. 26. 27. 28. 29. 30. 31. 32. 33. 34. 35. 36. 37. 38. 39. 40. 41. 42. 43. 44. 45.
15 16
Injector: Sample: Purge: Preheat: Desorb: Line temp: Detector:
1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14.
Dichlorofluoromethane Chloromethane Vinyl chloride Bromomethane Chloroethane Trichlorofluoromethane 1,1-Dichloroethene Methylene Chloride trans-1,2-Dichloroethene 1,1-Dichloroethane 2,2-Dichloropropane Bromochloromethane Chloroform 1,1,1-Trichloroethane 7
9 8
Ideal for 5973 MSD
Carbon tetrachloride Benzene Fluorobenzene Trichloroethene 1,2-Dichloropropane Dibromomethane Bromodichloromethane cis-1,3-Dichloropropene Toluene trans-1,3-Dichloropropene 1,1,2-Trichloroethane Tetrachloroethene Dibromochloromethane 1,2-Dibromomethane Chlorobenzene 1,1,1,2-Tetrachloroethane Ethylbenzene m-Xylene p-Xylene o-Xylene Bromoform Isopropylbenzene Bromofluorobenzene Bromobenzene n-Propylbenzene 2-Chlorotoluene 1,3,5-Trimethylbenzene tert-Butylbenzene 1,2,4-Trimethylbenzene sec-Butylbenzene 1,3-Dichlorobenzene
46. 47. 48. 49. 50. 51. 52. 53.
48 32,33 41
4-Isopropyltoluene 1,4-Dichlorobenzene 1,2-Dichlorobenzene 1,2-Dibromo-3Chloropropane 1,2,4-Trichlorobenzene Hexachlorobutadiene Napthalene 1,2,3-Trichlorobenzene
29 34 46
39 31 36 40 23
44 42 43
45 51
26 30 38 37
47
50 53
11
18
6 23 4 1 5
13 10 12
14
19 21 17 20
22 24 25 52 27 28 35 49
GC and GC/MS
8 Time (min)
10
11
12
13
14
15
C804
375

EPA Method 504 by GC/MS
Column:
P/N: Oven: Injector:
EPA Method 504 by GC/MS

Column:
P/N: Oven: Injector:
DB-VRX
30 m x 0.25 mm id, 1.4 m 122-1534 40C for 0.5 min 40-200C at 10/min Splitless, 200C 12 sec purge activation time 25 pg on-column of each component MSD, 220C transfer line, SIM
DB-624
30 m x 0.25 mm id, 1.4 m 122-1334 40C for 0.5 min 40-200C at 10/min Splitless, 200C 12 sec purge activation time 25 pg on-column of each component MSD, 220C transfer line, SIM 6. 7. 8. 9. 10. 11. Bromodichloromethane 1,2-Dibromopropane 1,2-Dibromoethane (EDB) Dibromochloromethane Bromoform 1,2-Dibromo-3-chloropropane (DBCP)
Detector: 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11.
Detector: 1. 2. 3. 4. 5.
1,1,1-Trichloroethane Carbon tetrachloride Trichloroethene Chloroform Tetrachloroethene Bromodichloromethane 1,2-Dibromopropane 1,2-Dibromoethane (EDB) Dibromochloromethane Bromoform 1,2-Dibromo-3-chloropropane (DBCP)
1,1,1-Trichloroethane Carbon tetrachloride Trichloroethene Chloroform Tetrachloroethene
GC and GC/MS
C373
C196
EPA Method 551

Column:
P/N: Carrier: Oven:
DB-1
30 m x 0.25 mm id, 1.0 m 122-1033 Helium at 24.8 cm/sec, measured at 150C 35C for 9 min 35-40C at 10/min 40C for 3 min 40-150C at 6/min 150C for 1 min Splitless, 200C 15 sec purge activation time 1 L of 50 pg/L, AccuStandard ECD, 300C
Injector:
Detector:
1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. 18. 19.
Chloroform 1,1,1-Trichloroethane Carbon tetrachloride Trichloroacetonitrile Dichloroacetonitrile Bromodichloromethane Trichloroethene Chloral hydrate 1,1-Dichloro-2-propanone Chloropicrin Dibromochloromethane Bromochloroacetonitrile 1,2-Dibromoethane Tetrachloroethene 1,1,1-Trichloropropanone 1,2-Dibromopropane (IS) Bromoform Dibromoacetonitrile 1,2-Dibromo-3-chloropropane
C201
376

EPA Method 551.1
Column:
P/N: Carrier: Oven:
DB-5ms
30 m x 0.25 mm id, 1.0 mm 122-5533 Helium at 35 cm/sec, measured at 150C 35C for 9 min 35-40C at 10/min 40C for 3 min 40-150C at 15/min 150C for 5 min Splitless, 200C 0.1 min purge activation time 1 mL of 0.5-10 ng/mL ECD, 325C
Injector:
Detector: 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. 18. 19. 20. 21.
Chloroform 1,1,1-Trichloroethane Carbon tetrachloride Trichloroacetonitrile Trichloroethene Bromodichloromethane Chloral hydrate Dichloroacetonitrile 1,1-Dichloro-2-propanone 1,1,2-Trichloroethane Chloropicrin Dibromochloromethane Tetrachloroethene 1,2-Dibromoethane Bromochloroacetonitrile 1,1,1-Trichloro-2-propanone Bromoform Dibromoacetonitrile 1,2,3-Trichloropropane p-Bromofluorobenzene (IS) 1,2-Dibromo-3-chloropropane
GC and GC/MS
C587
European Red List Volatiles

Column:
DB-5.625
30 m x 0.25 mm id, 0.5 m 122-5632 Helium at 35 cm/sec, measured at 40C 40C for 2 min 40-140C at 12/min Split 1:50, 250C 1 L of headspace of neat mixture FID, 300C Nitrogen makeup gas at 30 mL/min
Column:
DB-624
30 m x 0.25 mm id, 1.4 m 122-1334 Helium at 35 cm/sec, measured at 40C 40C for 2 min 40-140C at 12/min Split 1:50, 250C 1 L of headspace of neat mixture FID, 300C Nitrogen makeup gas at 30 mL/min
1. 2. 3. 4. 5. 6. 7. 8.
1,1-Dichloroethylene 1,1-Dichloroethane Chloroform 1,1,1-Trichloroethane 1,2-Dichloroethane Carbon tetrachloride Trichloroethylene Tetrachloroethylene
1. 2. 3. 4. 5. 6. 7. 8.
1,1-Dichloroethylene 1,1-Dichloroethane Chloroform 1,1,1-Trichloroethane 1,2-Dichloroethane Carbon tetrachloride Trichloroethylene Tetrachloroethylene
5 2 4 3 7 8
4 Time (min)
10
C307
C308
377

Environmental-Air Analysis
EPA Air Analysis Compendium Method TO-14 Standard
Column:
P/N: Carrier: Oven:
DB-1
60 m x 0.32 mm id, 1.0 m 123-1063 Helium at 25 cm/sec measured off of CO2 at 35C constant flow mode 35C for 5 min 35-120C at 5/min 120-220C at 30/min 220C for 5 min Entech 7100 cryogenic sample preconcentrator 400 mL of a 10 ppbV TO-14 standard and 100 mL of a 20 ppbV IS/SS standard MSD, full scan of m/z 40-250
Injector:
Detector:
Agilent wishes to thank Entech Instruments for providing this chromatogram.
1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. 18. 19. 20. 21. 22. 23.
CO2 Freon 12 (Dichlorodifluoromethane) Chloromethane Freon 114 (1,2-Dichloro-1,1,2,2-tetrafluoroethane) Vinyl chloride Bromomethane Chloroethane Freon 11 (Trichlorofluoromethane) 1,1-Dichloroethene Methylene chloride Freon 113 (1,1,2-Trichloro-1,2,2-trifluoroethane) 1,1-Dichloroethane cis-1,2-Dichloroethene Bromochloromethane (IS) Chloroform 1,2-Dichloroethane 1,1,1-Trichloroethane Benzene Carbon tetrachloride 1,4-Difluorobenzene (IS) 1,2-Dichloropropane Trichloroethene cis-1,3-Dichloropropene
24. 25. 26. 27. 28. 29. 30. 31. 32. 33. 34. 35. 36. 37. 38. 39. 40. 41. 42. 43. 44. 45. 46.
trans-1,3-Dichloropropene 1,1,2-Trichloroethane Toluene-d8 (SS) Toluene 1,2-Dibromoethane Tetrachloroethene Chlorobenzene-d5 (SS) Chlorobenzene Ethylbenzene m-Xylene p-Xylene Styrene 1,1,2,2-Tetrachloroethane o-Xylene 4-Bromofluorobenzene (SS) 1,3,5-Trimethylbenzene 1,2,4-Trimethylbenzene 1,3-Dichlorobenzene 1,2-Dichlorobenzene 1,4-Dichlorobenzene 1,2,4-Trichlorobenzene 1,2-Dibromobenzene (IS) Hexachloro-1,3-butadiene
11 22 17 15 8 9 10 14 3 5 6 7 13 12 16 20 23 21 18 19
GC and GC/MS
8 Time (min)
10
11
12
13
14
Ion 91.00 (90.70 to 91.70): 98100107.D
Peak 37 m/z 91 46
Peak 36 m/z 83
39
40 43 41
21.45 21.50 21.55 21.60 21.65 29 32 27 25 31 35 38 30 26 28 24 33
42
34
36 37 44 45
16
17
18
19
20
C731
21 22 Time (min)
23
24
25
26
27
378

EPA Air Analysis Method TO-15 (1 ppbV Standard)
Column:
P/N: Carrier: Oven: Peak # 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. 18. 19. 20. 21. 22. 23. 24. 25. 26. 27. 28. 29. 30. 31. 32. 33. 34. 35. 36. 37. 38. 39. 40. 41. 42. 43. 44. 45. 46. 47. 48. 49. 50. 51. 52. 53. 54. 55. 56. 57. 58. 59. 60. 61. 62. 63. 64. 65. 66. 67. 68. 69. 70. 71. Compound Name Formaldehyde Propene Dichlorodifluoromethane Chloromethane Dichlorotetrafluoroethane Acetaldehyde Vinyl chloride 1,3-Butadiene Bromomethane Chloroethane Bromoethene Trichlorofluoromethane Acetone Propanal Isopropyl alcohol 1,1-Dichloroethene 1,1,2-Trichloro-1,2-2-trifluoroethane Methylene chloride 3-Chloro-1-propene (Allyl chloride) Carbon disulfide trans-1,2-Dichloroethene tert-Butyl methyl ether (MTBE) 1,1-Dichloroethane Vinyl acetate 2-Butanone (MEK) n-Hexane cis-1,2-Dichloroethene Ethyl acetate Bromochloromethane <ISTD> Chloroform Tetrahydrofuran 1,1,1-Trichloroethane 1,2-Dichloroethane Benzene Carbon tetrachloride Cyclohexane 1,4-Difluorobenzene <ISTD> 2,2,4-Trimethylpentane (Isooctane) n-Heptane Trichloroethene 1,2-Dichloropropane 1,4-Dioxane Bromodichloromethane 4-Methyl-2-pentanone (MIBK) cis-1,3-Dichloropropene trans-1,3-Dichloropropene Toluene 1,1,2-Trichloroethane 2-Hexanone Dibromochloromethane Tetrachloroethene 1,2-Dibromoethane Chlorobenzene-d5 <ISTD> Chlorobenzene Ethylbenzene m-Xylene p-Xylene Styrene o-Xylene Bromoform 1,1,2,2-Tetrachloroethane 4-Bromofluorobenzene <SMC> 4-Ethyltoluene 1,3,5-Trimethylbenzene 1,2,4-Trimethylbenzene 1,3-Dichlorobenzene Benzyl chloride 1,4-Dichlorobenzene 1,2-Dichlorobenzene 1,2,4-Trichlorobenzene Hexachlorobutadiene Quantitation Ion 30 41 85 50 85 29 62 39 94 64 106 101 58 29 45 61 101 49 76 76 96 73 63 43 72 57 96 43 128 83 42 97 62 78 117 56 114 57 41 130 63 88 83 43 75 75 91 97 43 129 166 107 117 112 91 91 91 104 91 173 83 95 105 105 105 146 91 146 146 180 225
DB-5ms
60 m x 0.32 mm id, 1.0 m 123-5563 Helium, 1.5 mL/min 35C for 5 min 35-140C at 6C/min 140-220C at 15C/min 220C for 3 min Entech 7100 cryogenic sample preconcentrator 40 cc 10 ppb GC/MS 6890/5973 N Scan 29-180 amu 0-6 min 33-280 amu 6-30 min Electron Impact 70 eV Sample: 400 mL sample load, All compounds at 10 ppbV except Formaldehyde (50 ppbV), Acetaldehyde (20 ppbV), Propanol (20 ppbV), Acetone (30 ppbV), 2-Butanone (30 ppbV)
Injector: Sample: Detector:
37 29
4,5
17 40
34,35 3 12 18,19 13 6,7 8 1,2 10 9 11 14 15 28 31 16 20 21 23,24 22 33 26 27 25 30 32 36
38 39 43 44,45
41
GC and GC/MS
42
4 C1089-a
9 Time (min)
10
11
12
13
14
53
62
71
63 64 55 56 57 51 47 54 58 61
65 66
68 69 70
59
48 50 52 60
67
46 49
15 C1089-b
16
17
18
19
20
21 22 Time (min)
23
24
25
26
27
28
379

50ppb Formaldehyde
Column:
P/N: Carrier: Oven: Injector: Sample: Detector:
Sulfur in Air
Column:
P/N: Carrier: Oven: 1. 2. 3. 4. 5. 6. Formaldehyde Acetaldehyde Acetone-d6 Acetone Propanol 2-Butanone
Injector: Sample: Detector:
DB-5ms
60 m x 0.32 mm id, 1.0 m 123-5563 Helium, 1.5 mL/min 35C for 5 min 35-85C at 10C/min Entech 7100 cryogenic sample preconcentrator 100 cc 50 ppb Formaldehyde/20 ppb others GC/MS 6890/5973 N Scan 29-180 amu 0-6 min 33-280 amu 6-10 min Electron Impact 70 eV
DB-5ms
60 m x 0.32 mm id, 1.0 m 123-5563 Helium, 1.5 mL/min 35C for 5 min 35-140C at 6C/min 140-220C at 15C/min 220C for 3 min 5
Entech 7100 cryogenic sample preconcentrator 400 cc 10 ppb Sulfurs GC/MS 6890/5973 N Scan 29-180 amu 0-6 min 33-280 amu 6-30 min Electron Impact 70 eV
1. 2. 3. 4. 5. 6. 7. 8. 9.
Hydrogen Sulfide Carbonyl Sulfide Methyl Mercaptan Dimethyl Sulfide Carbon Disulfude Bromochloromethane 1,4-Difluorobenzene Chlorobenzene-d5 4-Bromofluorobenzene

8 9
2 7
6 4 2 5 1
GC and GC/MS
3.5
C1078
4.0
4.5
5.0
5.5 6.0 6.5 Time (min)
7.0
7.5
8.0
8.5
C1087
10
12 14 Time (min)
16
18
20
C1 and C2 Halocarbons (Freons)

Column:
P/N: Carrier: Oven:
GS-GasPro
Injector:
Detector:
60 m x 0.32 mm id 113-4362 Helium at 35 cm/sec constant velocity 40C for 2 min, 40-120C at 10/min 120C for 3 min 120-200C at 10/min Splitless, 250C 0.20 min purge activation time, 1.0 L of 100 ppm mixture of Accustandard M-REF & M-REF-X in methanol MSD, 280C, full scan 45-180 amu
Compound 1. Chlorotrifluoromethane (peak not shown) 2. Trifluoromethane 3. Bromotrifluoromethane 4. Chloropentafluoroethane 5. Pentafluoroethane 6. 1,1,1-Trifluoroethane 7. Dichlorodifluoromethane 8. Chlorodifluoromethane 9. 1,1,1,2-Tetrafluoroethane 10. Chloromethane 11. 1,1,2,2-Tetrafluoroethane 12. Bromochlorodifluoromethane
Freon # 13 23 13B1 115 125 143a 12 22 134a 40 134 12B1
Compound 13. 1,1-Difluoroethane 14. 1,2-Dichloro-1,1,2,2-tetrafluoroethane 15. 2-Chloro-1,1,1,2-tetrafluoroethane 16. 1-Chloro-1,1-difluoroethane 17. Dichlorofluoromethane 18. Trichlorofluoromethane 19. Chloroethane 20. Dichloromethane 21. 1,1-Dichloro-1-fluoroethane 22. 2,2-Dichloro-1,1,1-trifluoroethane 23. 1,1,2-Trichloro-1,2,2-trifluoroethane 24. 1,2-Dibromo-1,1,2,2-tetrafluoroethane
Freon # 152a 114 124 142b 21 11 160 141b 123 113 114B2
C664
380

Food and Flavors
Food and Flavors

Fragrance Reference Standard
Column:
DB-1
30 m x 0.25 mm id, 0.25 m 122-1032 Helium at 25 cm/sec, measured at 150C 40C for 1 min 40-290C at 5/min Split 1:50, 250C 1 L of a 1:20 dilution of neat sample in acetone MSD, 300C transfer line
Many thanks to Carl Frey, Manager of Analytical Services, Dragoco, and Kevin Myung, Director of Flavor and Perfumery Research, Bush Boake Allen, Inc. for contributing to this work.
1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. 18. 19. 20. 21. 22. 23. 24. 25. 26. 26a. 26b. 26c. 28. 29. 30. 31. 32. 33. 34. 35. 37. 38. 39. 41. 42.
Acetone 2,3-Butanedione (diacetyl) Ethyl acetate 2,3-Pentanedione (acetyl propionyl) Ethyl propionate Methyl butyrate 3-Methylbutyl alcohol 2-Methylbutyl alcohol Isobutyl acetate Ethyl butyrate Furfural Ethyl isovalerate Hexanol Allyl butyrate Ethyl pentanoate Hexylene glycol -Thujone Benzaldehyde -Pinene Camphene 3,5,5-Trimethylhexanol Sabinene -Pinene Ethyl hexanoate Myrcene Hexyl acetate cis-Linalool oxide Methyl benzoate trans-Linalool oxid Methyl-cresol Benzyl alcohol para-Cymene 1,8-Cineol Limonene 2,6-Dimethylhept-5-enal -Terpinene Octanol Ethyl heptanoate Linalool Benzene ethanol Rose oxide, cis-rose Rose oxide, trans-rose
43. 44. 45. 46. 47. 48. 49. 51. 52. 53. 54. 56. 57. 58. 59. 59a. 60. 61. 62. 63. 64. 66. 67. 68. 69. 70. 71. 72. 73. 74. 76. 78. 79. 80. 81. 82. 83. 85. 88. 89. 90. 91.
Camphor Citronellal Benzyl acetate Menthone Isoborneol Isomenthone Borneol Terpinen-4-ol -Terpineol Ethyl octanoate Octyl acetate Fenchyl acetate Citronellol Neral Carvonel Phenylethyl acetate Geraniol Linalyl acetate Geranial Hydroxycitronellal Citronellyl formate Bornyl acetate Vertenex (isomer 1) Ethyl nonanoate Geranyl formate Vertenex (isomer 2) -Nonalactone Citronellyl acetate Neryl acetate Geranyl acetate Diphenyl oxide Ethyl decanoate -Copaene Florazone (isomer 1) Florazone (isomer 2) -Caryophyllene Citronellyl propionate 3,7-Guaiadiene Dodecanol Ethyl undecanoate Eugenyl acetate Frambione (raspberry ketone)
93. Isoamyl salicylate 94. -Cadinene 95. cis-Nerolidol 96. Rosatol (rosetone) 96a. Geranyl butyrate 97. trans-Nerolidol 98. n-Amyl salicylate 99. Phenylethyl tiglate 100. Ethyl dodecanoate 101. Benzophenone 102. Dibenzyl ether 103. -Dodecalactone 104. Citronellyl tiglate 105. Evernyl 106. Geranyl tiglate 107. Geranyl-2-methyl valerate 108. Celestocide 109. Heptadec-1-ene 110. Benzyl benzoate 111. Ethyl tetradecanoate 112. Benzyl salicylate 113. Tonalid 114. Nonadec-1-ene 115. Isopropylmyristate 116. Ethyl pentadecanoate 116a. Nonadecane 117. Ethyl hexadecanoate 118. Musk T (ethylene brassylate) 119. Eicosane 120. Cinnamyl phenyl acetate 121. Heneicosane 122. Phenyl ethyl cinnamate 123. Ethyl octadecanoate 124. Hercolyn D (tetrahydro & dihydro methyl abietate) 125. Cinnamyl cinnamate 126. Cetearyl octanoate 127. Cetearyl decanoate
GC and GC/MS
C389
381

Food and Flavors
Fragrance Reference Standard
Column:
P/N: Carrier: Oven: 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. 18. 19. 20. 21. 22. 23. 24. 25. 26. 27. 28. 29. 30. 31. 32. 33. 34. 35. 36. 37. 38. 39. Acetone Ethyl acetate Ethyl propionate 2,3-Butanedione (diacetyl) Methyl butyrate Isobutyl acetate -Pinene Ethyl butyrate 2,3-Pentanedione (acetyl propionyl) Camphene Ethyl isovalerate -Pinene Ethyl pentanoate Myrcene Allyl butyrate Limonene 1,8-Cineol 3,5,5-Trimethylhexanol 3-Methylbutyl alcohol 2-Methylbutyl alcohol Ethyl hexanoate -Terpinene p-Cymene Hexyl acetate Terpinolene Ethyl heptanoate 2,6-Dimethylhept-5-enal (MelonalTM) Rose oxide, cis-rose Hexanol Rose oxide, trans-rose Methyl-para-cresol Ethyl octanoate cis-Linalool oxide Menthone Furfural trans-Linalool oxide Octyl acetate Isomenthone -Copaene 40. 41. 42. 43. 44. 45. 46. 47. 48. 49. 50. 51. 52. 53. 54. 55. 56. 57. 58. 59. 60. 61. 62. 63. 64. 65. 66. 67. 68. 69. 70. 71. 72. 73. 74. 75. 76. 77. 78. Camphor Benzaldehyde Ethyl nonanoate Linalool Linalyl acetate Vertenex (isomer 1) Octanol -Caryophyllene Vertenex (isomer 2) Terpinen-4-ol Methyl benzoate Hexylene glycol Ethyl decanoate Citronellyl acetate Isoborneol Neral -Terpineol Geranyl formate Borneol -Bisabolene Benzyl acetate Neryl acetate Geranial Ethyl undecanoate -Cadinene Geranyl acetate Citronellol Ethyl dodecanoate Geraniol Benzyl alcohol Geranyl butyrate Nonadecane Benzene ethanol Nonadec-1-ene Florazone (isomer 1) Florazone (isomer 2) Hydroxycitronellal Dodecanol Diphenyl oxide 79. 80. 81. 83. 84. 85. 86. 87. 90. 91. 92. 93. 94. 95. 96. 97. 98. 99. 100. 101. 102. 103. 104. 105. 106. Citronellyl tiglate Eugenyl methyl ether -Nonalactone Ethyl tetradecanoate n-Amyl salicylate Geranyl tiglate Ethyl pentadecanoate Isopropylmyristate Phenylethyl tiglate Rosatol (rosetone) Eugenyl acetate Ethyl hexadecanoate -Dodecalactone Dibenzyl ether Tonalid Ethyl octadecanoate Benzophenone Benzyl benzoate Cetearyl octanoate Musk T (ethylene brassylate) Cetearyl decanoate Frambione (raspberry ketone) Cinnamyl phenyl acetate Phenyl ethyl cinnamate Cinnamyl cinnamate
DB-WAX
30 m x 0.25 mm id, 0.25 m 122-7032 Helium at 25 cm/sec, measured at 150C 45C for 2 min 45-250C at 3/min 250C for 34 min Split 1:50, 250C 1 L of a 1:20 dilution of neat sample in acetone MSD, 250C transfer line
Injector:
Detector:
Many thanks to Carl Frey, Manager of Analytical Services, Dragoco, and Kevin Myung, Director of Flavor and Perfumery Research, Bush Boake Allen, Inc. for contributing to this work.
GC and GC/MS
C390
382

Food and Flavors
Perfume
Column: HP-INNOWax
P/N: Carrier : Oven: 30 m x 0.25 mm id, 0.25 m 19091N-133 Helium, 30 cm/sec 0.9 mL/min constant flow 80C for 1 min 80-250C at 5C/min 250C for 2 min Split 10:1, 250C MSD 280C
Flavor Mixture
Column: Ultra 2
P/N: Carrier: Injector: Oven: 25 m x 0.32 mm id, 0.52 m 19091B-112 Helium, 90 kPa., 2.2 mL/min constant flow Split 20:1, 250C 80C for 1 min 80-210C at 8C/min 210C for 2 min IRD, 280C, Wide Band MCT, 550 to 4000 cm-1
Injector: Detector:
1. 2. 3. 4. 5. 6. 7. 8. 9. 10.
Limonene Linalool Linalyl acetate Benzyl acetate Citronellol Benzene ethanol -Methyl Ionone Carvocrol and geraiol Isoamyl salicylate n-Amyl salicylate
11. 12. 13. 14. 15. 16. 17. 18. 19.
Commamyl acetate Acetylcedrene Diethyl phthalate Tonalid Coumarin Musk xylene Benzyl benzoate Benzyl salicylate Musk ketone
Detector:
1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13.
Fenchone Thujone Benzaldehyde trans-Carveol Farnesol cis-Carveol trans-Geraniol Citral Eugenol Vanillan trans-Isoeugenol trans-Citronellyl tiglate cis-Citronellyl tiglate
13 9 18 6 4 3 2 1 5 7 11 8 10 2 12
15 12 14
16 17 1 4 3 19 5 7 68 11 0 C1060 5 10 Time (min) 15 10
GC and GC/MS
C1056
10
15
20 Time (min)
25
30
Separation of Chiral Compounds Commonly Found in Essential Oils and Fragrances

Column:
P/N: GC: Carrier: Oven: Injection: Sample: Detection:
Menthol
Column:
1,2-Dimethylbenzene Myrcene (-)-Camphene (+)-Camphene (+)--pinene 1S-(-)--pinene Cineole (R)-(+)-Citronellal 1S,2R,5S-(+)-Menthol 1R,2S,5R-(-)-Menthol -Terpineol (+/-)-isoborneol (+)-borneol trans-Cinnamaldehyde P/N: Carrier: Oven: Injector: Detector:
Cyclodex-B
30 m x 0.25 mm id, 0.25 m, 112-2532 Hydrogen, 55 cm/sec 105 C isothermal Split ratio 1:100, 250C 1 L of 1 g/L each chloroform FID, 300C Nitrogen makeup gas at 30 mL/min
HP-Chiral-20 b
30 m x 0.25 mm id, 0.25 m, 19091G-B233 6890 Hydrogen, 39 cm/sec, Constant pressure 65 C for 1 min 65-170 C at 5 C/min Split ratio 30:1, 1 L, 250C 0.25 ng/L each analyte in Hexane FID, 300C 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14.
1. 2. 3. 4.
(+)-Neomenthol (-)- Neomenthol (+)-Menthol (-)-Menthol
14
7 5 6
9 10 13 11 12
4 3
C1079
10 Time (min)
15
20
C082
383

Food and Flavors
Garlic Oil
Column:
P/N: Guard Column: Carrier : Oven: 1. 2. 3. 4. 5. 6. 7. 8. 9. Allymethylsulfide Methyldisulfide Allylsulfide Allylmethyldisulfide cis-1-(Methyldithio)-1-propene Methylpropyldisulfidde l trans-1-(Methyldithio)-1-propene Dimethyltrisulfide Dipropenyldisulfide 10. 11. 12. 13. 14. 15. 16. 17. 18. Allylpropenyldisulfide Allyldisulfide Dipropyldisulfide Allylmethyltrisulfide Methylpropyltrisulfide 3-Vinyl-1,2-dithiin 2-Vinyl-1,3-dithiin Dimethyltetrausulfide Allyltrisulfide
DB-XLB
30 m x 0.25 mm id, 0.5 m 122-1236 1 m x 0.53 mm id deactivated tubing Helium at 41 cm/sec, measured at 45C 45C for 3 min 45-240C at 5/min 240C for 3 min Hot On-column, 100C 108 mL/min split vent flow 1.2 L dichloromethane plug with 1.2 L of 1.0 mg/mL sample of garlic oil in dichloromethane MSD, 200C transfer line full scan 35-215 amu
Injector:
Detector:
GC and GC/MS
C461
Lemon Oil
Column:
P/N: Carrier : Oven:
DB-5
20 m x 0.10 mm id, 0.1 m 127-5022 Hydrogen at 60 cm/sec, measured at 40C 40C for 3 min 40-185C at 30/min 185C for 3 min Split 1:275, 275C Nitrogen makeup gas at 30 mL/min
Injector: Detector:
1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14.
-Thujone -Thujone Camphene Sabinene -Pinene Myrcene Octanal -Phellandrene -Terpinene r-Cymene -Limonene -Trepinene Octanol Terpinolene
15. 16. 17. 18. 19. 20. 21. 22. 23. 24. 25. 26. 27. 28.
Linalool Nonanal Citronellal Trepinen-4-ol -Trepineol Decanal Neral Geranial Nerylacetate Geranylacetate -Caryophyllene trans--Bergamotene -Humulene -Bisabolene
Faster Analysis Time With 0.1 mm ID Columns!
C519
384

Food and Flavors
Cold-Pressed Orange Oil
Column:
P/N: Carrier : Oven: 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. -Pinene Sabinene Myrcene Octanal Limonen Linalool Nonanal Citonellal -Terpineol Decanal 11. 12. 13. 14. 15. 16. 17. 18. 19. Neral Geranial Dodecenal Valencene Cadinene -Sinensal -Sinensal Nootkatone Polymethoxyflavones
DB-5
20 m x 0.1 mm id, 0.1 m 127-5022 Hydrogen at 60 cm/sec, measured at 70C 70C for 1 min 70-250C at 30/min 250-310C at 20/min 310C for 2 min Split 1:275, 275C FID, 350C Nitrogen makeup gas at 30 mL/min
Injector: Detector:
Chromatogram courtesy of Tastemaker
GC and GC/MS
C521
Peppermint Oil
Column:
DB-WAX
60 m x 0.25 mm id, 0.25 m 122-7062 Helium at 25 cm/sec (0.73 mL/min) 75C for 8 min 75-200C at 4/min 200C for 5 min Split 1:150, 270C 1L neat FID, 270C Nitrogen makeup gas at 30 mL/min
Injector: Detector:
1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15.
-Pinene -Pinene Sabinene Myrcene -Terpinene /-Limonene 1,8-Cineol cis-Ocimene Terpinene r -Cymene -Terpinolene 3-Octanol 1-Octen-3-ol trans-Sabiene hydrate /-Methone
16. 17. 18. 19. 20. 21. 22. 23. 24. 25. 26. 27. 28. 29.
Methofuran d-Isomethone -Bourbonene Linalool Methylacetate Neomenthol Trepinen-4-ol -Caryophyllene /-Menthol Pulegone -Terpineol Germacrene-D Piperitone Viridiflorol
Thanks to: Mr. William Faas of A.M. Todd Company for providing the sample and assisting with peak identification.
C210
385

Food and Flavors
Spearmint Oil (Western)
Column:
DB-WAX
60 m x 0.25 mm id, 0.25 m 122-7062 Helium at 25 cm/sec (0.73 mL/min) 75C for 8 min 75-200C at 4/min 200C for 5 min Split 1:150, 270C 1 L neat FID, 270C Nitrogen makeup gas at 30 mL/min
Injector: Detector:
1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15.
-Pinene -Pinene Sabinene Myrcene -Terpinene /-Limonene 1,8-Cineol cis-Ocimene -Terpinene r-Cymene Terpinolene 3-Octylacetate 3-Octanol trans-Sabiene hydrate /-Methone
16. 17. 18. 19. 20. 21. 22. 23. 24. 25. 26. 27. 28. 29. 30.
-Bourbonene Linalool Trepinen-4-ol -Caryophyllene Dihydrocarvone trans-Dihydrocarvyl trans- -Farnesene -Terpineol Germacrene-D /-Carvone cis-Carvylacetate trans-Carveol cis-Carveol cis-Jasmone Viridiflorol
Thanks to: Mr. William Faas of A.M. Todd Company for providing the sample and assisting with peak identification.
GC and GC/MS
C209
Ylang Ylang Oil

Column:
P/N: Carrier : Oven: Injector: Detector:
DB-XLB
30 m x 0.25 mm id, 0.25 m 122-1232 Helium at 34 cm/sec, measured at 50C 50C for 1 min 50-250C at 3.5/min Split 1:125, 250C 1L of 10% oil in methylenechloride MSD, full scan at m/z 35-550 310C transfer line
1. r-Methylansiole 2. Linalool 3. Methylbenzoate 4. Benzylacetate 5. Geraniol 6. Geranylacetate 7. -Caryophyllene 8. Cinnamylacetate 9. Germacrene-D 10. Benzylbnzoate 11. Faneosolacetate 12. Benzylsalicylate
C577
386

Food and Flavors
Rosemary Oil
Column:
P/N: Carrier : Oven: Injector: Detector:
CycloSil-B
30 m x 0.25 mm id, 0.25 m 112-6632 Hydrogen at 40 cm/sec, measured at 60C 55C for 1 min 50-180C at 5/min Split 50:1, 250C FID, 340C 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. (+/-) -Pinene (+/-) Camphene (+/-) -Pinene (+/-) Limonene (+/-) Linalool (+/-) Camphor (+/-) Terpinene-4-ol (+/-) Isoborneol (+/-) Borneol (+/-) -Terpineol
GC and GC/MS
C509
Citrus Flavored Carbonated Beverage (Soda)

Column:
P/N: Carrier : Oven: Detector: SPME Fiber: Extraction:
CycloSil-B
30 m x 0.25 mm id, 0.25 m 112-6632 Helium at 37 cm/sec, measured at 40C 40-190C at 2 min HP5973MSD, 280C transfer line Ployacrylate fiber, 85 m Headspace, no stir, NaCl 1g/10 mL sample 68 min at 27C Adsorption 250C for 15 min, Split 1:5
1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11 12 13
S(-)Limonene p-Cymene R(+)Limonene Octanol -Terpinene Nonanol 2-Ethyl-1-Hexanol Linalool Decanol Terpinen-4-ol Phenethylalcohol -Terpineol BHT
Desorption
C740
387

Food and Flavors
Alcohol Beverage Standard
Column: HP-FFAP
P/N: Carrier: Injection: Oven: 50 m x 0.2 mm id, 0.33 m 19091F-105 Hydrogen Split, 100:1, 1 L 60C for 4 min 60-200C at 6C/min 200C for 2 min FID 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. Acetaldehyde Acetone Ethyl formate Ethyl acetate Acetal Methanol Ethanol Diacetyl sec-butanol n-propanol Isobutanol n-butanol Isoamyl alcohol n-amyl alcohol Acetic acid Propionic acid
Bourbon
Column: HP-INNOWax
P/N: Carrier: Oven: 30 m x 0.25 mm id, 0.25 m 19091N-133 Helium, 33 cm/sec, 15.5 psi (35C) 1.5 mL/min constant flow 35C for 5 min 35-150C at 5C/min 150-250C at 20C/min 250C for 2 min Split 25:1, 220C,1 L FID 280C
Detector:
1. 2. 3. 4. 5. 6. 7. 8.
Acetaldehyde Ethyl acetate Methanol Ethanol Acetic acid n-Propanol Isobutanol 2-Methyl-1-butanol or 3-Methyl-1-Butanol
11 10 9 2 5 4 2 3 8 7 13 14 15 1 3 5 12 8
GC and GC/MS
C1050
20 min
C1052
10 Time (min)
20
30
Alditol Acetates
Column: DB-225
P/N: Carrier: Oven: Injector: Detector: 30 m x 0.25 mm id, 0.15 m 122-2231 Hydrogen a 36.5 cm/sec 220C isothermal Split, 1:50, 1 L, 225C FID, 250C Nitrogen makeup gas at 30 mL/min
Strawberry Syrup
Column: HP-INNOWax
P/N: Carrier: Oven: 30 m x 0.32 mm id, 0.5 m 19091N-213 Helium, 40 cm/sec, 11.7 psi (60C) 2.5 mL/min constant flow 60C for 1 min 60-250C at 10C/min 250C for 2 min Split 60:1, 220C FID 275C
Injector: Detector: 1. 2. 3. 4. 5. 6. 7. 8. Rhamnitol Fucitol Ribitol Arabinitol Mannitol Galactitol Glucitol Inositol
1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12.
Ethyl acetate Ethyl butyrate Isoamyl acetate Amyl acetate Isoamyl butyrate Amyl butyrate Ethyl benzoate Citronellol Geraniol Ethyl-3-phenyl oxiran carboxylate Strawberry aldehyde Benzyl benzoate
2 3 4
11
10
5 7 8 9 12
0 C085 C1062
10 Time (min)
15
20
388

Food and Flavors
Sulfur and Selenium in Garlic by Headspace
Column:
P/N: Carrier:
HP-INNOWax
60 m x 0.32 mm id, 0.25 m 19091N-116 (A): Helium, 28 cm/sec, 18 psi (35C), 1.2 mL/min constant flow (B): Helium, 28 cm/sec, 5.6 psi (35C), 1.2 mL/min constant flow 35C for 3 min 35-230C at 5C/min 230C for 5 min Splitless, SI inlet (140C), AED 190C and MSD 230C
Oven:
Injector: Detector:
1. CH3-Se-CH3 2. C3H5-S-CH3 3. C3H5-Se-CH3 4. CH3-S-S-CH3 5. C3H5-S-C3H5 6. CH3-S-Se-CH3 7. C3H5-S-S-CH3 7a. CH3-Se-Se-CH3
8. 9. 10. 11. 12. 13. 14.
CH3-Se-S-C3H5 CH3-S-S-S-CH3 C3H5-S-S-C3H5 CH3-S-Se-S-CH3 CH3-S-S-S-C3H5 C3H5-S-S-S-C3H5 C3H5-S-S-S-C3H5
(A) AED
(B) MSD
5 2 10
10 2 4 5 6 8 7 9
12
13 7 12 13
1 3 1 0
C1069
4 3 6 10 89 11 25
7a 10 Time (min) 20
11 30
C1070
GC and GC/MS
15 20 Time (min)
Free Fatty Acids

Column: HP-INNOWax
30 m x 0.25 mm id, 0.25m 19091N-133 Helium 42 cm/sec, 24 psi (120C) 1.8 mL/min constant flow 120C for 1 min 120-250C at 10C/min 250C for 5 min Split 40:1, 250C, 1 L 0.05 to 0.11% each in methylene chloride FID 280C
TMS Sugars
Column:
DB-1701
30 m x 0.25 mm id, 0.25 m 122-0732 Hydrogen at 40 cm/sec 180C for 2 min 180-200C at 5/min Split 1:50, 225C FID, 250C Nitrogen makeup gas at 30 mL/min
P/N: Carrier: Oven:
Injection: Sample: Detector:
1. 2. 3. 4. 5. 6. 7. 8. 9.
D-Arabinose D-Ribose anomers D-Zylose D-Mannose D-Fructose -D-Galactose -D-Glucose D-Fructose (anomer) -D-Glucose
1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. 18.
C2 C3 C4 C5 C5-Iso C6 C7 C8 C10 C12 C14 C14:1 C16 C16:1 C18 C18:1 C18:2 C18:3
1 9 8 34 5 6 7 10 11
14 16
12 13 15 17 18
C086
0
C1047
15 Time (min)
389

Food and Flavors
Organic Acids
Column:
P/N: Carrier: Oven:
DB-FFAP
30 m x 0.25 mm id, 0.25 m 122-3232 Helium at 40 cm/sec, measured at 100C 100C for 5 min 100-250C at 10/min 250C for 12 min Split 1:50, 250C FID, 300C Nitrogen makeup gas at 30 mL/min
Injector: Detector:
1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11.
Acetone Formic acid Acetic acid Propionic acid Isobutyric acid Butyric acid Isovaleric acid Valeric acid (pentanoic acid) Isocaproic acid Caproic acid (hexanoic acid) Heptanoic acid
12. 13. 14. 15. 16. 17. 18.
Octanoic acid Decanoic acid Dodecanoic acid Tetradecanoic acid Hexadecanoic acid Octadecanoic acid Arachidic acid (eicosanoic acid)
GC and GC/MS
C077
Bacterial Fatty Acid Methyl Esters

Column:
DB-5
30 m x 0.25 mm id, 0.25 m 122-5032 Hydrogen at 42 cm/sec 150C for 4 min 150-250C at 4/min Split 1:100 FID Nitrogen makeup gas at 30 mL/min Methylundecanoate Methyl 2-hydroxydecanoate Methyl laurate Methyl tridecanoate Methyl 2-hydroxydodecanoate Methyl 3-hydroxydodecanoate Methyl myristate Methyl 12-methyltetradecanoate Methyl pentadecanoate Methyl 2-hydroxytetradecanoate Methyl 3-hydroxytetradecanoate Methyl palmitoleate Methyl palmitate Methyl 14-methylhexadecanoate Methyl cis-9,10-methyl hexadecanoate Methyl heptadecanoate Methyl 2-hydroxyhexadecanoate Methyl oleate Methyl elaidate Methyl stearate Methyl cis-9,10-methylene octadecanoate Methyl nonadecanoate Methyl arachidate
1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. 18. 19. 20. 21.
C11:0 2-OH C10:0 C12:0 C13:0 2-OH C12:0 3-OH C12:0 C14:0 12-Me C14:0 C15:0 2-OH C14:0 3-OH C14:0 C16:1 C16:0 14-Me C16:0 9,10-diMe C16:0 C17:0 2-OH C16:0 C18:1 C18:1 C18:0 9,10-diMe C18:0
22. C19:0 23. C20:0
C068
390

Food and Flavors
FAMES
Column:
P/N: Carrier: Hydrogen at 43 cm/sec Constant pressure mode 130C for 1.0 min 130-170C at 6.5C/min 170-215C at 2.75C/min 215C for 12 min 215-230C at 40C/min 230C for 3 min Split, 270C Split ratio 50:1 FID, 280C 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. 18. 19. 20. 21. C6:0 C7:0 C8:0 C9:0 C10:0 C11:0 C12:0 BHT C13:0 C14:0 C14:1n5 C15:0 C16:0 C16:1n7(trans) C16:1n7(cis) C17:0 C17:1 C18:0 C18:1n9(trans) C18:1n9(cis) C18:1n7 22. 23. 24. 25. 26. 27. 28. 29. 30. 31. 32. 33. 34. 35. 36. 37. 38. 39. 40. 41. C18:2n6 C18:3n6 C18:3n3 C18:2(d9,11) C18:2(d10,12) C20:0 C20:1n9 C20:2n6 C20:3n6 C20:4n6 C20:3n3 C20:5n3 C22:0 C22:1n9 C22:2n6 C22:4n6 C22:5n3 C24:0 C22:6n3 C24:1n9
DB-23
60 m x 0.25 mm id, 0.25 m 122-2362
Oven:
6 9 8 5 3 4 1 2 10 11 13 15 16 17 14 7 12
Injector: Detector:
Baseline Resolution of C16 and C18 cis-, trans- isomers
20
19 21
18
22
23 24
2526 27
28
29 30
3132
Short Analysis Time 33 34 35 36 37 383940 41
GC and GC/MS
2
C796
10
12
14 Time (min)
16
18
20
22
24
26
28
Chromatogram provided courtesy of Steve Watkins and Jeremy Ching, FAME Analytics, http://www.fameanalytics.com
Polyunsaturated Fatty Acid Methyl Esters

Column:
P/N: Sample: Carrier: Oven:
HP-INNOWax
30 m x 0.32 mm id, 0.5 m 19091N-213 50 g/L in methylene chloride Helium, 30 cm/sec, 10.7 psi (150C) 1.5 mL/min constant flow 150C for 1 min 150-200C at 15C/min 200-250C at 2C/min 250C for 5 min Split (60:1), 0.5 L, inlet 220C FID, 275C 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. C14:0 C16:0 C16:1w7 C18:0 C18:1w9 C18:1w7 C18:2w6 C18:3w3 C20:1w9 C20:2w6 C20:3w6 C20:4w6 C20:5w3 C22:4w6 C22:5w3 C22:6w3

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4 6 5 2
12 14
7 3 8 1 9 10 11 13 15 16
0
C1045
10
15 Time (min)
20
25
391

Food and Flavors
FAME Standard
Column:
P/N: Carrier: Oven: 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. Butyric acid methyl ester (C4:0) Caproic acid methyl ester (C6:0) Caprylic acid methyl ester (C8:0) Capric acid methyl ester (C10:0) Undecanoic acid methyl ester (C11:0) Lauric acid methyl ester (C12:0) Tridecanoic acid methyl ester (C13:0) Myristic acid methyl ester (C14:0) Myristoleic acid methyl ester (C14:1) Pentadecanoic acid methyl ester (C15:0) cis-10-Pentadecenoic acid methyl ester (C15:1) Palmitic acid methyl ester (C16:0) Palmitoleic acid methyl ester (C16:1) 14. 15. 16. 17. 18. 19. 20. 21. 22. 23. 24. 25. 26. 27. 28. 29. 30. 31. 32. 33. 34. 35. 36. 37. Heptadecanoic acid methyl ester (C17:0) cis-10-Heptadecenoic acid methyl ester (C17:1) Stearic acid methyl ester (C18:0) Oleic acid methyl ester (C18:1n9c) Elaidic acid methyl ester (C18:1n9t) Linoleic acid methyl ester (C18:2n6c) Linolelaidic acid methyl ester (C18:2n6t) -Linolenic acid methyl ester (C18:3n6) Linolenic acid methyl ester (C18:3n3) Arachidic acid methyl ester (C20:0) cis-11-Eicosenoic acid methyl ester (C20:1) cis-11,14-Eicosadienoic acid methyl ester (C20:2) cis-8,11,14-Eicosatrienoic acid methyl ester (C20:3n6) Heneicosanoic acid methyl ester (C21:0) cis-11,14,17-Eicosatrienoic acid methyl ester (C20:3n3) Arachidonic acid methyl ester (C20:4n6) cis-5,8,11,14,17-Eicosapentaenoic acid methyl ester (C20:5n3) Behenic acid methyl ester (C22:0) Erucic acid methyl ester (C22:1n9) cis-13,16-Docosadienoic acid methyl ester (C22:2) Tricosanoic acid methyl ester (C23:0) Lignoceric acid methyl ester (C24:0) cis-4,7,10,13,16,19-Docosahexaenoic acid methyl ester (C22:6n3) Nervonic acid methyl ester (C24:1)
DB-WAX
10 m x 0.10 mm id, 0.10 m 127-7012 Hydrogen at 77 cm/sec, measured at 40C 40C for 0.5 min 40-195C at 25/min 195-205C at 3/min 205-230C at 8/min 230C for 1 min Split 1:30, 250C FID, 250C
Injector: Detector:
Excellent Resolution in 12 Minutes!
GC and GC/MS
C583
FAME Standard
Column:
P/N: Carrier: Oven:
DB-225
20 m x 0.10 mm id, 0.10 m 127-2222 (cut to 10 m) Hydrogen at 59.3 cm/sec, measured at 35C 35 for 0.5 min 35-195C at 25/min 195-205C at 3/min 205-230C at 8/min 230C for 1 min Split 1:30, 250C FID, 250C
1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13.
Butyric acid methyl ester (C4:0) Caproic acid methyl ester (C6:0) Caprylic acid methyl ester (C8:0) Capric acid methyl ester (C10:0) Undecanoic acid methyl ester (C11:0) Lauric acid methyl ester (C12:0) Tridecanoic acid methyl ester (C13:0) Myristic acid methyl ester (C14:0) Myristoleic acid methyl ester (C14:1) Pentadecanoic acid methyl ester (C15:0) cis-10-Pentadecenoic acid methyl ester (C15:1) Palmitic acid methyl ester (C16:0) Palmitoleic acid methyl ester (C16:1)
Injector: Detector:
14. 15. 16. 17. 18. 19. 20. 21. 22. 23. 24. 25. 26. 27. 28. 29. 30. 31. 32. 33. 34. 35. 36.
Heptadecanoic acid methyl ester (C17:0) cis-10-Heptadecenoic acid methyl ester (C17:1) Stearic acid methyl ester (C18:0) Oleic acid methyl ester (C18:1n9c) Elaidic acid methyl ester (C18:1n9t) Linoleic acid methyl ester (C18:2n6c) Linolelaidic acid methyl ester (C18:2n6t) -Linolenic acid methyl ester (C18:3n6) Linolenic acid methyl ester (C18:3n3) Arachidic acid methyl ester (C20:0) cis-11-Eicosenoic acid methyl ester (C20:1) cis-11,14-Eicosadienoic acid methyl ester (C20:2) cis-8,11,14-Eicosatrienoic acid methyl ester (C20:3n6) Heneicosanoic acid methyl ester (C21:0) cis-11,14,17-Eicosatrienoic acid methyl ester (C20:3n3) Arachidonic acid methyl ester (C20:4n6) cis-5,8,11,14,17-Eicosapentaenoic acid methyl ester(C20:5n3) Behenic acid methyl ester (C22:0) Erucic acid methyl ester (C22:1n9) cis-13,16-Docosadienoic acid methyl ester (C22:2) Tricosanoic acid methyl ester (C23:0) Lignoceric acid methyl ester (C24:0) cis-4,7,10,13,16,19-Docosahexaenoic acid methyl ester (C22:6n3) 37. Nervonic acid methyl ester (C24:1)
C582
392

Food and Flavors
Canola Oil Margarine Partially Hydrogenated FAMEs AOCS Method 1c-89
Column:
P/N: Carrier: Oven: Injector: Detector: 1. 2. 3. 4. 5. 6. 7. 8. 9. 10.
DB-23
60 m x 0.25 mm id, 0.25 m 122-2362 Helium at 15 cm/sec (0.44 mL/min), measured at 150C 150-200C at 1.3/min 200C for 10 min Split 1:100, 1 L, 210C FID, 210C
C16:0 Methyl palmitate C16:1 Methyl palmitoleate C18:0 Methyl stearate C18:1 trans-Methyl elaidate and Multiple isomers C18:1 cis-Methyl oleate and Multiple isomers C18:2 trans-Multiple isomers C18:2 cis-Multiple isomers C18:3 Methyl linolenate C20:0 Methyl arachidate C20:1 Methyl 11-eicosanoate
C211
GC and GC/MS
Butter Triglycerides
Column:
P/N: Carrier: Oven:
Butter Triglycerides
Column:
DB-5ht
30 m x 0.32 mm id, 0.1 m 123-5731 Hydrogen at 55 cm/sec, measured at 250C 35-250C at 70/min 250-400C at 5/min 400C for 20 min Cool On-column 1 L of 9 g/L in toluene (approx. 1% w/w solution) FID, 400C Nitrogen makeup gas at 30 mL/min Baseline Corrected
DB-17ht
30 m x 0.32 mm id, 0.15 m 123-1831 Hydrogen at 40 cm/sec 250-365C at 5/min 365C for 1 min Cool On-column 1 L of 9 g/L in toluene (approx. 1% w/w solution) FID, 400C Nitrogen makeup gas at 30 mL/min Baseline Corrected
Injector:
Detector:
Detector:
T= Total number of Carbons
Peak Identification: P=Palmitic acid, hexadecanoic acid, C16:0 S=Stearic acid, octadecanoic acid, C18:0 O=Oleic acid, cis-9-octadecanoic acid, C18:1
C079
C212
393

Industrial Chemicals
Alcohols
Column:
P/N: Carrier: Oven:
DB-624
-Prosteel version 30 m x 0.53 mm id, 3.0 m 125-1334 Helium at 30 cm/sec, measured at 40C 40C for 5 min 40-260C at 10C/min 260C for 3 min Split 1:10, 250C 1 L of 0.01-0.05% each solvent in CS2 FID, 300C Nitrogen makeup gas at 30 mL/min
3, CS2 7,8 1
Injector: Detector:
1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13.
Methanol Ethanol Isopropanol tert-Butanol 2-Propen-1-ol (allyl alcohol) 1-Propanol 2-Propyn-1-ol (propargyl alcohol) sec-Butanol 2-Methyl-3-buten-2-ol Isobutanol 2-Methoxyethanol (methyl Cellosolve) 3-Buten-1-ol 2-Methyl-2-butanol (tert-amyl alcohol)
14. 15. 16. 17. 18. 19. 20. 21. 22. 23. 24. 25. 26.
1-Butanol 2-Buten-1-ol (crotyl alcohol) Ethylene glycol 1-Penten-3-ol 2-Pentanol Glycidol 3-Pentanol 2-Ethoxyethanol (Cellosolve) Propylene glycol 3-Methyl-1-butanol (isoamyl alcohol) 2-Methyl-1-butanol (active amyl alcohol) 4-Methyl-2-pentanol 1-Pentanol
22,29,30
37,39
41,42,43 44
49,50,52
18,20 28 26,27 24 19 15 17 11 13 12 14 10 9 23 21 16 25 31 34 36 35 32 40 38 33 45 47 46 51 48
27. 28. 29. 30. 31. 32. 33. 34. 35. 36. 37. 38. 39. 40. 41. 42. 43. 44. 45. 46. 47. 48. 49. 50. 51. 52.
2-Penten-1-ol 3-Methyl-2-buten-1-ol Cyclopentanol 3-Hexanol 2-Hexanol 4-Hydroxy-4-methyl-2-pentanone Furfuryl alcohol cis-3-Hexen-1-ol 1-Hexanol cis-2-Hexen-1-ol Cyclohexanol 3-Heptanol 2-Heptanol 2-Butoxyethanol (butyl Cellosolve) cis-4-Hepten-1-ol trans-2-Hepten-1-ol 1-Heptanol Benzyl alcohol 2-Ethyl-1-hexanol a-Methylphenyl alcohol 1-Octanol 1-Nonanol 2-Phenoxyethanol a-Ethylphenethyl alcohol b-Ethylphenethyl alcohol 1-Decanol
5 2 4 6
GC and GC/MS
10 Time (min)
15
20
C397
Alcohols
Column:
P/N: Carrier: Oven:
DB-WAXetr
50 m x 0.32 mm id, 1.0 m 123-7354 Helium at 50 cm/sec, measured at 40C 40C for 5 min 40-230C at 10/min 230C for 5 min Split 1:5, 250C 1 L of 0.15% each solvent in CS2 FID, 250C Nitrogen makeup gas at 35 mL/min
Injector:
14. 15. 16. 17. 18. 19. 20. 21. 22. 23. 24. 25.
1-Butanol 2-Buten-1-ol (crotyl alcohol) Ethylene glycol 1-Penten-3-ol 2-Pentanol Glycidol 3-Pentanol 2-Ethoxyethanol (Cellosolve) Propylene glycol 3-Methyl-1-butanol (isoamyl alcohol) 2-Methyl-1-butanol (active amyl alcohol) 4-Methyl-2-pentanol
26. 27. 28. 29. 30. 31. 32. 33. 34. 35. 36. 37.
1-Pentanol 2-Penten-1-ol 3-Methyl-2-buten-1-ol Cyclopentanol 3-Hexanol 2-Hexanol 4-Hydroxy-4-methyl-2-pentanone Furfuryl alcohol cis-3-Hexen-1-ol cis-2-Hexen-1-ol Cyclohexanol 3-Heptanol
Detector:
38. 39. 40. 41. 42. 43. 44. 45. 46. 47. 48. 49. 50.
2-Heptanol 2-Butoxyethanol (butyl Cellosolve) cis-4-Hepten-1-ol trans-2-Hepten-1-ol 1-Heptanol Benzyl alcohol 2-Ethyl-1-hexanol 1-Octanol 1-Nonanol 2-Phenoxyethanol a-Ethylphenethyl alcohol b-Ethylphenethyl alcohol 1-Decanol
1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13.
Methanol Ethanol Isopropanol tert-Butanol 2-Propen-1-ol (allyl alcohol) 1-Propanol 2-Propyn-1-ol (propargyl alcohol) sec-Butanol 2-Methyl-3-buten-2-ol Isobutanol 2-Methoxyethanol (methyl Cellosolve) 3-Buten-1-ol 2-Methyl-2-butanol (tert-amyl alcohol)
C550
394

Alcohols
Column:
P/N: Carrier: Oven:
Ethoxyethanol
HP-INNOWax
30 m x 0.53 mm id, 1.0 m 19095N-123 Helium, 29 cm/sec, 3.0 psi (45C) 45C for 1 min 45-150C at 10C/min 4 mL/min constant flow Split (25:1), 250C, 1 L FID 250C 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 1-Propanol iso-Butanol 3-Methyl-3-pentanol 1-Butanol 4-Methyl-2-pentanol 1-Pentanol 2-Ethyl-1-Butanol 1-Hexanol Cyclohexanol 1-Heptanol
Column:
P/N. Carrier: Oven:
HP-FFAP
30 m x 0.53 mm id, 1.0 m 19095F-123 Helium, 10 mL/min 60C for 1 min 60-100C at 5C/min 100-210C at 10C/min Split, 10:1 TCD
1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11.
Ethylene oxide Ethyl formate Ethyl alcohol Water 2-Ethoxyethanol 2-Ethoxyethyl acetate Hydroxy acetate Acetic acid Formic acid Ethylene glycol/monoformate Ethylene glycol/monoacetate
10
8 9 5 7
10
3 1 2
5 2 1
0
11 7
4 3
5 10 Time (min)
9
15 20
0 C817
6 Time (min)
10
12
GC and GC/MS
C845
Organic Acids
Column:
P/N: Carrier: Oven:
Free Organic Acids/C4-C5 Isomers

Column:
P/N: Oven:
DB-WAXetr
30 m x 0.53 mm id, 1.0 m 125-7332 Helium at 37 cm/sec, measured at 40C 125C for 5 min 125-180C at 15C/min 180C for 12 min Split, 250C FID, 250C
HP-INNOWax
30 m x 0.25 mm id, 0.25 m 19091N-133 110C for 1 min 110-133 at 2C/min 133-160C at 3C/min Helium 42 cm/sec, 24 psi (120C) 1.8 mL/min constant flow Split (40:1), 1 L, 250C FID 300C 1. 2. 3. 4. 5. 6. 7. 8. 9. 1 2 4 3 5 6 8 9 Isobutyric acid Butyric acid Valerolactone 2-Methyl butyric acid Valeric acid 4-Pentenoic acid trans-2-Methyl-2-butenoic acid trans-3-Pentenoic acid trans-2-Pentenoic acid
Carrier: Injection: Detector:
Injector: Detector: 1. 2. 3. 4. 5. 6. 7. 8. 9.
Acetic acid Propionic acid Isobutyric acid Butyric acid Isovaleric acid Valeric acid (pentanoic acid) Isocaproic acid Caproic acid (hexanoic acid) Heptanoic acid
0 C568
C814
10 Time (min)
15
20
395

Volatile Amines
Column:
P/N: Oven: Injector: Detector:
Primary Amines
Column:
P/N: Carrier: Oven: Sample: Detector:
DB-1
30 m x 0.53 mm id, 5.0 m 125-1035 30C isothermal Split 1:10, headspace injections FID Nitrogen makeup gas at 30 mL/min
HP-35
15 m x 0.25 mm id, 0.25 m 19091G-131 Helium, 1.5 mL/min constant flow 45C for 2 min 45-220C at 25C/min 1 L split (ratio 30:1), 0.1 to 0.35% aliphatic amines in methanol FID 310C
1. Methylamine 2. Dimethylamine 3. Trimethylamine
1. 2. 3. 4. 5. 6. 7. 8. 9.
n-Butylamine n-Amylamine n-Hexylamine n-Cyclohexylamine n-Heptylamine n-Octylamine n-Decylamine n-Dodecanylamine n-Tetradecanylamine
1 3
GC and GC/MS
C101
C822
6 Time (min)
10
Primary Amines
Column:
Polyethyleneamines
Column:
CAM
30 m x 0.25 mm id, 0.25 m 112-2132 Hydrogen at 40 cm/sec 110C isothermal Split FID Nitrogen makeup gas at 30 mL/min
DB-5ms
30 m x 0.25 mm id, 0.5 m 122-5536 Helium at 30 cm/sec, measured at 100C 100C for 1 min 100-320C at 20C/min Split 1:50, 250C 1 L of 100 ng/L standard in methanol FID, 300C Nitrogen makeup gas at 30 mL/min 1. Ethylenediamine 2. Diethylenetriamine 3. Triethylenetetramine 3.* Branched and piperazine analogs of peak #3 4. Tetraethylenepentamine 4.* Branched and piperazine analogs of peak #4
1. 2. 3. 4. 5.
n-Octylamine n-Nonylamine n-Decylamine Benylamine Dicyclohexylamine
C100
C398
396

Amines and Nitriles
Column:
DB-5ms
30 m x 0.25 mm id, 0.5 m 122-5536 Helium at 22 cm/sec, measured at 40C 40C for 1 min 40-260C at 10C/min Split 1:50, 250C 1 L of 100 ng/L standard in methanol FID, 300C Nitrogen makeup gas at 30 mL/min 27. 28. 29. 30. 31. 32. 33. 34. 35. 36. 37. 38. 4-Chloroaniline N,N-Diethylaniline n-Decylamine 4-Bromoaniline 3,4-Diaminotoluene 2,6-Diethylaniline 2-Nitroaniline Dicyclohexylamine 3,4-Dichloroaniline 3-Nitroaniline 4-Nitroaniline Diphenylaniline
1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. 18. 19. 20. 21. 22. 23. 24. 25. 26.
Diethylamine Propionitrile Diisopropylamine Triethylamine Pyridine Pyrimidine Pyrazole Acrylamide Pyridazine Aniline 3-Bromopyridine Benzonitrile 3-Cyanopyridine Benzylamine n-Octylamine 1-Methyl-2-pyrrolidine N,N-Dimethylbenzylamine Phenylethylamine N-Benzylmethylamine 2-Cyanopyridine 2-Chloroaniline n-Nonylamine 2,4-Dimethylaniline 4-Chlorobenzonitrile 2,6-Dimethylaniline 3-Chloroaniline
GC and GC/MS
C399
Amines in Water
Column:
Aldehydes and Acids

Column:
P/N: Carrier: Oven:
CAM
30 m x 0.25 mm id, 0.25 m 112-2132 Hydrogen at 38 cm/sec 120-220C at 10C/min Split FID Nitrogen makeup gas at 30 mL/min 1. 2. 3. 4. 5. 6. 7. Ethylenediamine Piperazine Diethylenetriamine N-(2-Aminoethyl) piperazine Aminoethylethanolamine Triethylenetetramine (4 isomers) Tetraethylenepentamine (4 isomers)
HP-INNOWax
30 m x 0.32 mm id, 0.5 m 19091N-213 Helium, 40 cm/sec, 11.7 psi (60C) 60C for 1 min 60-250C at 10C/min 2.5 mL/min constant flow Split (40:1), 0.5 L, 250C FID 275C
1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17.
Butanal 2-Methyl butanal Pentanal Hexanal Heptanal Octanal Acetic acid Decanal Propanoic acid iso-Butyric acid Butyric acid iso-Valeric acid Valeric acid Hexanoic acid Heptanoic acid Octanoic acid Decanoic acid
9 10 11 12 13 16 2 7 1 14 3 5 4 6 8 15 17
C099
C820
10 Time (min)
15
20
397

Aldehydes and Ketones
Column:
DB-1
30 m x 0.32 mm id, 3.0 m 123-1034 Helium at 32 cm/sec, measured at 40C 40C for 5 min 40-210C at 10C/min Split 1:100, 250C FID, 300C Nitrogen makeup gas at 30 mL/min
GC and GC/MS
Compound list for both chromatograms 1. Acetaldehyde 2. Acrolein 3. Acetone 4. Propionaldehyde 5. Isobutyraldehyde 6. Methacrolein 7. Butyraldehyde 8. 2-Butanone (MEK) 9. Crotonaldehyde 10. 3-Methyl-2-butanone 11. 2-Pentanone 12. 3-Pentanone 13. Valeraldehyde (pentanal) 14. 4-Methyl-2-pentanone (MIBK) 15. 2-Methyl-3-pentanone 16. 3-Hexanone 17. Cyclopentanone 18. 2-Hexanone 19. Hexanal 20. Furfural 21. 4-Heptanone 22. 3-Heptanone 23. 2-Heptanone 24. Cyclohexanone 25. Heptanal 26. Benzaldehyde 27. Octyl aldehyde 28. o-Tolualdehyde 29. m-Tolualdehyde 30. p-Tolualdehyde 31. Nonyl aldehyde
C396
DB-1 provides the best overall resolution; however, DB-WAX provides better resolution of o- and m-tolualdehyde.
Column:
DB-WAX
30 m x 0.32 mm id, 0.5 m 123-7033 Helium at 32 cm/sec, measured at 40C 40C for 5 min 40-210C at 10C/min Split 1:100, 250C FID, 300C Nitrogen makeup gas at 30 mL/min
C397
398

Formaldehyde Underivatized
Column:
DNPH Derivative
Column:
PFBHA Derivative
Column:
DB-WAX
30 m x 0.32 mm id, 0.5 m 123-7033 Helium at 36 cm/sec, measured at 35C 35C isothermal Split 1:100, 200C FID, 300C Nitrogen makeup gas at 30 mL/min
DB-1
15 m x 0.32 mm id, 0.25 m 123-1012 Helium at 35 cm/sec, measured at 150C 150-250C at 20C/min Split 1:100, 300C ECD, 375C Nitrogen makeup gas at 35 mL/min
DB-1
15 m x 0.32 mm id, 0.25 m 123-1012 Helium at 40 cm/sec, measured at 60C 60-100C at 10C/min Split 1:100, 250C FID, 375C Nitrogen makeup gas at 35 mL/min
1. Formaldehyde 2. Methanol *Formaldehyde by-products
1. Formaldehyde-DNPH
1. Formaldehyde-PFBHA
GC and GC/MS
C393
C394
C395
Aromatics
Column:
DB-1
Prosteel version 30 m x 0.53 mm id, 3.0 m 125-1034 Helium at 30 cm/sec, measured at 40C 40C for 5 min 40-260C at 10C/min Split 1:10, 250C FID, 300C Nitrogen makeup gas at 30 mL/min
1. 2. 3. 4. 5. 6. 7. 8. 9.
Benzene Fluorobenzene Toluene Chlorobenzene Ethylbenzene m-Xylene p-Xylene Styrene o-Xylene
10. 11. 12. 13. 14. 15. 16.
Isopropylbenzene (cumene) Bromobenzene Propylbenzene 2-Chlorotoluene 3-Chlorotoluene 4-Chlorotoluene 1,3,5-Trimethylbenzene (mesitylene)
17. -Methylstyrene 18. tert-Butylbenzene 19. 1,2,4-Trimethylbenzene (pseudocumene) 20. 4-Methylstyrene 21. 1,3-Dichlorobenzene 22. 1,4-Dichlorobenzene 23. Isobutylbenzene 24. sec-Butylbenzene 25. 1,2,3-Trimethylbenzene (hemimellitene) 26. 1,2-Dichlorobenzene 27. Iodobenzene 28. Styrene oxide 29. Butylbenzene 30. 4-Chlorostyrene 31. Nitrobenzene 32. 4-tert-Butyltoluene 33. 1,3,5-Trichlorobenzene 34. 2-Nitrotoluene 35. 1,3-Diisopropylbenzene 36. 1,4-Diisopropylbenzene 37. 1,2,4-Trichlorobenzene 38. 3-Nitrotoluene 39. 4-Nitrotoluene 40. 1,2,3-Trichlorobenzene 41. 1-Chloro-4-nitrobenzene 42. 1,2,4,5-Tetrachlorobenzene 43. Pentachlorobenzene
399

Aromatics
Column:
P/N: Carrier: Oven: 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. Benzene Fluorobenzene Toluene Chlorobenzene Ethylbenzene m-Xylene p-Xylene Styrene o-Xylene Isopropylbenzene (cumene) 11. Bromobenzene 12. Propylbenzene 13. 14. 15. 16. 17. 18. 19. 20. 21. 22. 23. 2-Chlorotoluene 3-Chlorotoluene 4-Chlorotoluene 1,3,5-Trimethylbenzene (mesitylene) -Methylstyrene tert-Butylbenzene 1,2,4-Trimethylbenzene (pseudocumene) 4-Methylstyrene 1,3-Dichlorobenzene 1,4-Dichlorobenzene Isobutylbenzene 24. sec-Butylbenzene 25. 1,2,3-Trimethylbenzene (hemimellitene) 26. 1,2-Dichlorobenzene 27. Iodobenzene 28. Styrene oxide (peak not shown) 29. Butylbenzene 30. 4-Chlorostyrene 31. Nitrobenzene 32. 4-tert-Butyltoluene 33. 1,3,5-Trichlorobenzene 34. 2-Nitrotoluene 35. 1,3-Diisopropylbenzene 36. 1,4-Diisopropylbenzene 37. 1,2,4-Trichlorobenzene 38. 3-Nitrotoluene 39. 4-Nitrotoluene 40. 1,2,3-Trichlorobenzene 41. 1-Chloro-4-nitrobenzene 42. 1,2,4,5-Tetrachlorobenzene 43. Pentachlorobenzene
DB-WAX
-Prosteel version 30 m x 0.53 mm id, 1.0 m 125-7032 Helium at 30 cm/sec, measured at 40C 40C for 5 min 40-230C at 10C/min 230C for 7 min Split 1:10, 250C FID, 300C Nitrogen makeup gas at 30 mL/min
Injector: Detector:
See below for enlarged view
GC and GC/MS
C553
400

Impurities in Styrene
Column:
DB-WAXetr
60 m x 0.32 mm id, 0.5 m 123-7363 Helium at 29.4 cm/sec, measured at 70C 80C isothermal Split 1:150, 230C FID, 240C
11 3 9
1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11.
Benzene Toluene Ethylbenzene p-Xylene m-Xylene Isopropylbenzene o-Xylene n-Propylbenzene Styrene -Methylstyrene Heptane (IS)
ASTM D5135
10 6
8 1 4 2 5 7
10 Time (min)
20
30
C588
GC and GC/MS
Impurities in Ethylbenzene
Column:
P/N: Carrier: Oven:
Impurities in Mixed Xylenes

1. 2. 3. 4. 5. 6. 7. 8. 9. 10. Hydrocarbon Benzene Toluene Ethylbenzene p-Xylene m-Xylene Cumene o-Xylene Propylbenzene Styrene
HP-INNOWax
60 m x 0.32 mm id, 0.5 m 19091N-216 Helium, 32 cm/sec, 19.9 psi (60C) 2.5 mL/min constant flow 60C for 1 min 60-92C at 4C/min 92C for 4.5 min 92-220C at 20C/min Split (100:1), 0.5 L, inlet 220C ASTM Method D5060 Neat, 99%+ FID 270C
Column:
P/N: Carrier: Oven:
DB-WAXetr
60 m x 0.32 mm id, 0.25 m 123-7362 Helium at 20 cm/sec, measured at 145C 60C for 10 min 60-150C at 5/min 150C for 10 min Split 1:150, 230C FID, 240C
Injector: Detector:
ASTM D2360
1. 2. 3. 4. 5. 6. 7. Benzene Toluene Ethylbenzene p-Xylene m-Xylene o-Xylene n-Butylbenzene (IS)
4
4
6
1 6 5 7 3 1 2 1 8 9 10 10 Time (min) 5 C848 10 Time (min) 15 C472 11
401

Pyrolysates of Polystyrene
Column:
P/N: Carrier: Oven: Injection: Sample: Detector:
Esters
Column:
1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. Propylene Propane 1-Butene Butene Pentane Toluene Styrene C2H5-C(Ph) = CH2 C4H9-CH2CH2-Ph C4H9-C(Ph) = CH2 C4H9-CH=C(Ph)CH3 Styrene dimer Styrene trimer P/N: Carrier: Oven: Injector: Detector:
HP-Ultra 1
50 m x 0.20 mm id, 0.33 m 19091A-105 Helium, 30 psi, 12 mL/min 0-280 at 5C/min Split 30:1, inlet 280C, Pyrolyzer 600C 100 mg pyrolyzed FID 300C
DB-1
-Prosteel version 30 m x 0.53 mm id, 3.0 m 125-1034 1. Helium at 30 cm/sec, 2. measured at 40C 3. 40C for 5 min 4. 40-260C at 10/min 5. Split 1:10, 250C 6. FID, 300C 7. Nitrogen makeup gas 8. at 30 mL/min 9. 10. 11. 12. 13. Methyl formate Ethyl formate Methyl acetate Vinyl acetate Ethyl acetate Propyl formate Methyl propionate Isopropyl acetate Ethyl acrylate tert-Butyl acetate Ethyl propionate Propyl acetate sec-Butyl acetate 14. 15. 16. 17. 18. 19. 20. 21. 22. 23. 24. Isobutyl acetate Propyl propionate Butyl acetate Isoamyl acetate Amyl acetate 2-Ethoxyethyl acetate 2-Methylbutyl acetate Methyl benzoate Benzyl acetate Ethyl benzoate Propyl benzoate
12
13 10 2 1 4 9 5 10 C835 20 8 11 30 Time (min) 40 50 60 C554
GC and GC/MS
3 0
Esters
Column:
P/N: Carrier: Oven:
DB-624
-Prosteel version 30 m x 0.53 mm id, 3.0 m 125-1334 Helium at 30 cm/sec, measured at 40C 40C for 5 min 40-260C at 10/min 260C for 3 min Split 1:10, 250C FID, 300C Nitrogen makeup gas at 30 mL/min
Injector: Detector:
9. 10. 11. 12. 13. 14. 15. 16. 17. 18. 19. 20. 21. 22. 23. 24.
Ethyl acrylate tert-Butyl acetate Ethyl propionate Propyl acetate sec-Butyl acetate Isobutyl acetate Propyl propionate Butyl acetate Isoamyl acetate Amyl acetate 2-Ethoxyethyl acetate 2-Methylbutyl acetate Methyl benzoate Benzyl acetate Ethyl benzoate Propyl benzoate
Esters
Column: HP-INNOWax
P/N: Carrier: Oven: Injection: Detector: 30 m x 0.53 mm id, 1.0 m 19095N-123 Helium 29 cm/sec, 3.0 psi (45C) 4 mL/min constant flow 45C for 1 min 45-200C at 5C/min Split (25:1), 1 L, inlet 250C FID 250C
1. 2. 3. 4. 5. 6. 7. 8.
Methyl formate Ethyl formate Methyl acetate Vinyl acetate Ethyl acetate Propyl formate Methyl propionate Isopropyl acetate 1
1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. 18. 19.
Ethyl propionate Propyl acetate Ethyl butyrate Propyl propionate Propyl butyrate Ethyl valerate Butyl propionate Propyl valerate Ethyl caproate Butyl valerate Propyl caproate Methyl decanoate Butyl caproate Methyl dodecanoate Butyl heptanoate Methyl tetradecanoate Methyl hexadecanoate Methyl octadecanoate Methyl eicosenoate
6 2 3 4 5 8
9 15 11 13
10 12 7 14 16 17 18 19
0 C558 C824
10
15 Time (min)
20
25
402

Ethers
Column:
P/N: Carrier: Oven: 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. Furan Ethyl vinyl ether Ethyl ether 1,3-Dioxolane Methyl-tert-butyl ether (MTBE) Allyl ethyl ether Isopropyl ether Tetrahydrofuran (THF) tert-Butyl ethyl ether Butyl methyl ether Glyme (propylene glycol dimethyl ether) tert-Amyl methyl ether 13. 14. 15. 16. 17. 18. 19. 20. 21. 22. 23. 24. Diglyme (diethylene glycol dimethyl ether) Propyl ether Allyl ether 1,4-Dioxane Butyl ethyl ether Epichlorohydrin Tetrahydropyran Acetal (acetaldehyde diethyl acetal) Butyl ether Pentyl ether Triglyme (triethylene glycol dimethyl ether) Benzyl ether
DB-624
Injector: Detector:
GC and GC/MS
C558
Glycols
Column:
DB-WAX
30 m x 0.45 mm id, 0.85 m 124-7032 Helium at 35 cm/sec, measured at 50C 50C for 2 min 50-220C at 10/min Megabore Direct, 1 L, 250C FID, 280C Nitrogen makeup gas at 30 mL/min
1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. 18. 19.
Ethylene glycol monomethyl ether Ethylene glycol monoethyl ether 1,3-Propanediol 1,2-Propanediol (propylene glycol) 2,3-Butanediol 1,3-Butanediol Ethylene glycol monobutyl ether Diethylene glycol monomethyl ether 1,4-Butanediol Diethylene glycol monoethyl ether Dipropylene glycol 1,5-Pentanediol 1,6-Hexanediol 1,7-Heptanediol Diethylene glycol monobutyl ether 1,8-Octanediol 1,9-Nonanediol Ethylene glycol 1,10-Decanediol
Glycols Column:
P/N: Carrier: Oven:
DB-624
Injector: Detector:
1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. 18. 19. 20. 21. 22.
Ethylene glycol monomethyl ether Glyme Ethylene glycol Diglyme Ethylene glycol monoethyl ether 1,3-Propanediol 1,2-Propanediol (propylene glycol) 2,3-Butanediol 1,3-Butanediol Ethylene glycol monobutyl ether 1,4-Butanediol Diethylene glycol monomethyl ether Diethylene glycol Diethylene glycol monoethyl ether 1,5-Pentanediol 1,6-Hexanediol Diethylene glycol monobutyl ether Triglyme 1,7-Heptanediol 1,8-Octanediol 1,9-Nonanediol 1,10-Decanediol
C405
C561
403

Glycols
Column:
Triethylene Glycol and Impurities

DB-1
-Prosteel version 30 m x 0.45 mm id, 1.27 m 124-1032 Helium at 35 cm/sec, measured at 50C 50C for 2 min 50-260C at 10/min Megabore Direct, 1 L, 250C FID, 280C Nitrogen makeup gas at 30 mL/min 1. 2. 3. 4. 5. 6. 7. 8. 9. Ethylene glycol monomethyl ether Ethylene glycol monoethyl ether 1,3-Propanediol 1,2-Propanediol 2,3-Butanediol 1,3-Butanediol Ethylene glycol monobutyl ether Diethylene glycol monomethyl ether 1,4-Butanediol 10. 11. 12. 13. 14. 15. 16. 17. 18. 19. Diethylene glycol monoethyl ether Dipropylene glycol 1,5-Pentanediol 1,6-Hexanediol 1,7-Heptanediol Diethylene glycol monobutyl ether 1,8-Octanediol 1,9-Nonanediol Ethylene glycol 1,10-Decanediol
Column:
DB-1
30 m x 0.45 mm id, 1.27 m 124-1032 Helium at 35 cm/sec, measured at 50C 170C isothermal Split 1:50, 0.5 L, 250C FID, 280C Nitrogen makeup gas at 30 mL/min
1. Ethylene glycol 2. Diethylene glycol 3. Triethylene glycol
GC and GC/MS
C404
C406
Ethylene Glycol Mixture

Column:
Glycols/Diols
Column:
P/N: Carrier: Pressure program: Oven: 1. 2. 3. 4. 5. Diethylene glycol Triethylene glycol Polyethylene glycol 200 Polyethylene glycol 400 Polyethylene glycol 600 Injection: Inlet program: Detector:
Ultra-1
12 m x 0.2 mm id, 0.33 m 19091A-101 Helium, 25 cm/sec 100C for 0.5 min 100-200C at 20C/min Split (100:1), 250C, 1 L FID
HP-1
30 m x 0.53 mm id, 0.88 m 19095Z-023 Helium 10 psi to 25 psi (4.25 min) at 1 psi/min 50C for 3 min 50-180C at 8C/min On-column, 1 L 50C (0 min) to 180C (8.45 min) at 12C/min FID 250C 2 1 1. 2. 3. 4. 5. 6. Ethylene glycol 1,3-Butanediol Ethylene glycol phenyl ether 1,7-Hepatanediol 1,9-Nonanediol 1,10-Decanediol
1 3
4
5 2 4 5 6
7 min 20 min
C818
C819
404

Halogenated Hydrocarbons
Column:
P/N: Carrier: Oven: Injector: Detector: 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. Pentane Iodomethane 1,1-Dichloroethene 1,1,2-Trichlorotrifluoroethane (Freon-113) 3-Chloropropene (allyl chloride) Methylene chloride 1,1-Dichloroethane Chloroform 1,1,1-Trichloroethane 1-Chlorobutane Carbon tetrachloride 1,2-Dichloroethane 13. 14. 15. 16. 17. 18. 19. 20. 21. 22. 23. 24. 25. 26. 27. 28. 1,2-Dichloropropane cis-1,2-Dichloropropene trans-1,2-Dichloropropene 1,1,2-Trichloroethane 1,1,1,2-Tetrachloroethane 1,2-Dibromoethane (EDB) 1-Chlorohexane trans-1,4-Dichloro-2-butene Iodoform Hexachlorobutadiene 1,2,3-Trichloropropane 1,1,2,2-Tetrachloroethane Pentachloroethane 1,2-Dibromo-3-chloropropane (DBCP) Hexachloroethane Hexachlorocyclopentadiene
DB-624
30 m x 0.32 mm id, 1.8 m 123-1334 Helium at 35 cm/sec 35C for 5 min 35-245C at 10/min Split 1:50, 250C FID, 300C Nitrogen makeup gas at 30 mL/min
GC and GC/MS
C215
Halogenated Hydrocarbons
Column:
P/N: Carrier: Oven:
DB-1
30 m x 0.32 mm id, 3.0 m 123-1034 Helium at 35 cm/sec, measured at 35C 35C for 5 min 35-245C at 10/min 245C for 2 min Split 1:100 in pentane, 250C FID, 300C Nitrogen makeup gas at 30 mL/min
Injector: Detector:
1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. 18. 19. 20. 21. 22. 23. 24. 25. 26. 27. 28. 29. 30. 31. 32. 33. 34. 35. 36. 37. 38.
1,1,2-Trichlorotrifluoroethane (Freon-113) 1,1-Dichloroethene Bromoethane (ethyl bromide) Iodomethane 3-Chloropropene (allyl chloride) 1-Chlorobutane 2,2-Dichloropropane trans-1,2-Dichloroethene 1,1,1-Trichloroethane Carbon tetrachloride Methylene chloride Trichloroethene Chloroform Tetrachloroethene 1,2-Dichloropropane 1-Chlorohexane Bromochloromethane 1,1-Dichloroethane 1,2-Dichloroethane Iodoform cis-1,3-Dichloropropene Dibromomethane Bromodichloromethane 1,3-Dichloropropane 1,1-Dichloropropane trans-1,3-Dichloropropene 1,1,2-Trichloroethane 1,2-Dibromoethane (EDB) 1,1,1,2-Tetrachloroethane Pentachloroethane Hexachloroethane Bromoform trans-1,4-Dichloro-2-butene 1,2,3-Trichloropropane Hexachlorobutadiene 1,1,2,2-Tetrachloroethane 1,2-Dibromo-3-chloropropane (DBCP) Hexachlorocyclopentadiene
C113
405

Chlorinated Isooctane
Column:
HP-INNOWax
60 m x 0.25 mm id, 0.25 m 19091N-136 Helium, 33 cm/sec, 35.7 psi (80C) 2 mL/min 80C isothermal Split (150:1), 0.5 L, 250C 1. 1-Chloro isooctane FID 300C 2. 4-Chloromethyl 2,2'-dimethyl pentane 3. 3-Chloro isooctane 4. 4-Chloro isooctane

4 2 3
Monochloro isomers

6 7
GC and GC/MS
3 C829
4 Time (min)
Solvents
Column:
DB-WAXetr
30 m x 0.53 mm id, 1.0 m 125-7332 Helium at 30 cm/sec, measured at 40C 40C for 5 min 40-140C at 5/min Split, 250C FID, 250C
1. 2. 3. 4. 5. 6. 7. 8.
3-Methylpentane Hexane Isooctane Methyl-tert-butyl ether (MTBE) Heptane Cyclohexane Octane Nonane
9. 10. 11. 12. 13. 14. 15. 16. 17. 18.
Methanol Ethanol Benzene Decane Toluene Undecane Ethylbenzene p-Xylene m-Xylene Cumene
19. 20 21. 22. 23. 24. 25. 26. 27. 28. 29. 30.
Dodecane o-Xylene Propylbenzene Chlorobenzene Mesitylene Styrene 1,2,4-Trimethylbenzene Naphthalene 4-Chlorotoluene 1,3-Dichlorobenzene 1,4-Dichlorobenzene 1,2-Dichlorobenzene
C569
406

Solvents
Column:
DB-WAXetr
50 m x 0.32 mm id, 1.0 m 123-7354 Helium at 35.2 cm/sec, measured at 50C 50C for 5 min 50-170C at 10/min Split 1:100, 250C FID, 280C Nitrogen makeup gas at 30 mL/min
1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. 18. 19.
Hexane Isooctane Acetone Ethyl formate Tetrahydrofuran Trichloroethane Ethyl acetate Isopropyl acetate Methyl ethyl ketone Methylene chloride Isopropyl alcohol Benzene 2-Pentanone Methyl isobutyl ketone Isobutyl acetate Chloroform sec-Butyl alcohol Toluene n-Propanol
GC and GC/MS
C420
Aromatic Solvents
Column:
DB-200
30 m x 0.25 mm id, 0.25 m 122-2032 Helium at 31 cm/sec 50C for 5 min 50-160C at 10/min Split 1:100, 250C 0.5 L of 0.5 g/L standard in hexane FID, 300C Nitrogen makeup gas at 30 mL/min
Detector:
1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12.
Benzene Toluene Ethylbenzene Chlorobenzene p-Xylene m-Xylene o-Xylene Styrene Isopropylbenzene n-Propylbenzene 2-Chlorotoluene 3-Chlorotoluene
13. 14. 15. 16. 17. 18. 19. 20. 21. 22. 23. 24. 25.
4-Chlorotoluene tert-Butylbenzene sec-Butylbenzene Isobutylbenzene 1,3-Dichlorobenzene 1,4-Dichlorobenzene n-Butylbenzene 1,2-Dichlorobenzene 1,3-Diisopropylbenzene 1,4-Diisopropylbenzene 2-Nitrotoluene 3-Nitrotoluene 4-Nitrotoluene
C505
407

Solvents
Column:
P/N: Carrier: Oven: Injector: 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. 18. 19. 20. 21. 22. 23. 24. 21 23 24 1 9 14 20 19 22 Methanol Ethanol Ethyl ether Isopropanol n-Hexane Methylene chloride tert-Butanol n-Propanol Chloroform Cyclohexane sec-Butanol n-Heptane Acetone Acetonitrile Benzene Tetrahydrofuran (THF) Trichloroethylene n-Butanol Ethyl acetate Methyl ethyl ketone (MEK) Toluene 1,4-Dioxane Pyridine Dimethylformamide (DMF)
DB-200
30 m x 0.25 mm id, 0.5 m 122-2033 Helium at 31 cm/sec 45C for 7 min 45-145C at 20/min Split 1:00, 250C 0.5 L of 0.5-1.0 g/L standard in water FID, 300C Nitrogen makeup gas at 30 mL/min
11,12,13
Detector:
15 6 2,3 5 4 16 18 7 10 8 17
GC and GC/MS
0
C504
6 Time (min)
1 2
Solvents
Column:
HP-1
50 m x 0.2 mm id, 0.52 m 19091Z-205 Helium, 30 psi 70-200C at 5C/min 200C for 2 min Split, 1 L TCD
1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14.
Isopropanol Methyl ethyl ketone Ethyl acetate n-Butyl alcohol Ethyl cellosolve Methyl isobutyl ketone Toluene n-Butyl acetate Diacetone alcohol p-Xylene Cellosolve acetate o-Xylene Butyl cellosolve Butyl cellosolve acetate
Common Industrial Solvents

Column: HP-1
P/N: Carrier: Oven: Injection: Detector: 25 m x 0.32 mm id, 1.0 m 19091Z-212 Helium, 35 kPa 30-140C at 10C/min Split (200:1), 1 L IRD 200C 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. 18. 19. Methanol Methyl formate Ethanol Acetone Isopropanol Dichloromethane n-Propanol Methyl ethyl ketone sec-Butanol Ethyl acetate Isobutanol Isopropyl acetate Nitropropane 1,4-Dioxane Toluene Mesityl oxide Diacetone-alcohol m-Xylene Cyclohexanone
12 2 3 4 9 7 5 6 10 8 13 11 12 13 14 1 8 2 3 1 45 6 7 9 11 10
19 17
14
16 18 15
25 min C847 C839
12 min
408

Rapid Analysis of Water in Organic Solvents
Column:
P/N: Carrier: Oven: Post temp: Inlet: Injector: Injection volume: Detector:
Nitrogen Based Solvents

Column:
HP-INNOWax
15 m x .53 mm id, 1.0 m 19095N-121 15 mL/min helium constant flow mode 100C for 1.5 min 175C (2.5 min) Purge packed 240C 0.5 L, 87 ppm Water in Aniline Thermal conductivity (TCD), 240C
DB-1
-Prosteel Version 30 m x 0.53 mm id, 3.0 m 125-1034 Helium at 30 cm/sec, measured at 40C 40C for 5 min 40-260C at 10/min Split 1:10, 250C FID, 300C Nitrogen makeup gas at 30 mL/min
1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13.
Acetonitrile Acrolein Acrylonitrile Propionitrile Methacrolein Methacrylonitrile Triethylamine Ethyl acrylate Pyridine DMF (dimethylformamide) DMSO (dimethyl sulfoxide) Benzonitrile 1-Methyl-2-pyrrolidinone
12
CS 2 1
87 ppm Water in Aniline
13
7 8 10 11
0 C846
0.2
0.4
0.6
0.8 Time (min)
1.0
1.2
0
C565
8 10 Time (min)
12
14
16
18
GC and GC/MS
Nitrogen Based Solvents

Column:
P/N: Carrier: Oven:
DB-624
Injector: Detector:
1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13.
Acetonitrile Acrolein Acrylonitrile Propionitrile Methacrolein Methacrylonitrile Triethylamine Ethyl acrylate Pyridine DMF (dimethylformamide) DMSO (dimethyl sulfoxide) Benzonitrile 1-Methyl-2-pyrrolidinone
C566
409

Acrylate Impurities
Column:
P/N: Carrier: Oven: Injector: Detector: 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. 18. 19. Methanol Ethanol Methyl acetate Ethyl acetate Methyl acrylate Methyl propionate Isobutanol Butanol Ethyl acrylate Ethyl propionate Methyl methacrylate Isopropyl acrylate Isobutyl acetate Butyl acetate Isobutyl acrylate Dibutyl ether Isobutyl propionate Butyl acrylate Butyl propionate
Acrylate Impurities
Column:
DB-200
30 m x 0.53 mm id, 1.0 m 125-2032 Helium at 34.5 cm/sec measured at 35C 35C for 5 min, 35 - 200C at 10/min Split 1:10, 230C FID, 250C
DB-1701
-Prosteel version 30 m x 0.53 mm id, 1.0 m 125-0732 Helium at 36.8 cm/sec measured at 35C 35C for 5 min, 35-200C at 10/min Split 1:10, 230C FID, 250C
1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. 18. 19.
Methanol Ethanol Methyl acetate Ethyl acetate Methyl acrylate Methyl propionate Isobutanol Butanol Ethyl acrylate Ethyl propionate Methyl methacrylate Isopropyl acrylate Isobutyl acetate Butyl acetate Isobutyl acrylate Dibutyl ether Isobutyl propionate Butyl acrylate Butyl propionate
Methyl acrylate impurities * Ethyl acrylate impurities ] Butyl acrylate impurities
Methyl acrylate impurities Ethyl acrylate impurities ] Butyl acrylate impurities
GC and GC/MS
C694
C693
Acrylates
Column: HP-FFAP
P/N: Carrier: Oven: 10 m x 0.53 mm id, 1.0 m 19095F-121 Hydrogen 35C for 1 min 35-60C at 10C/min 60-160C at 15C/min On-column, 1 L FID
Monomers in Latex Paint by Headspace

Column:
P/N: Carrier: Oven: 1. 2. 3. 4. 5. 6. 7. 8. 9. Methyl methacrylate Ethyl methacrylate sec-Butyl methacrylate Allyl acrylate n-Butyl acrylate Hexyl methacrylate Cyclohexyl methacrylate Hydroxypropyl acrylate Unknown
HP-INNOWax
30 m x 0.53 mm id, 1.0 m 19095N-123 Helium, 45.5 cm/sec (40C) 6 mL/min constant flow 40C for 5 min 40-90C at 15C/min 90-200C at 30C/min Split (10:1), 1 mL headspace, inlet temp 220C 0.200 gm sample with 1 gram sodium sulfate in 5 mL water FID 300C
1. Acrylonitrile 2. Methyl acrylate 3. Butyl acrylate 4 Styrene
4 3
7 2 6 8
2 1
4 1 9
11 min C815 C834
6 Time (min)
10
12
410

Anilines
Column:
P/N: Carrier: Oven:
DB-35ms
25 m x 0.20 mm id, 0.33 m 128-3822 1 m x 0.32 mm id, retention gap Helium at 35 cm/sec, measured at 50C 50C for 2 min 50-340C at 20/min 340C for 10 min Splitless, 280C, 1 L of 5 ng on-column per component 0.50 min purge activation time FID, 320C Nitrogen makeup gas at 30 mL/min
Injector:
Detector:
1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17.
o-Toluidine 4-Chloroaniline 2-Methoxy-5-methylaniline 2,4,5-Trimethylaniline 4-Chloro-2-methylaniline 2,4-Diaminotoluene 2,4-Diaminoanisole 2-Aminonaphthalene 2-Methyl-5-nitroaniline 4,4-Oxydianiline 4,4-Methylenedianiline Benzidine 2-Aminoazotoluene o-Tolidine 4,4-Thiodianiline 3,3-Dimethoxybenzidine 3,3-Dichlorobenzidine
GC and GC/MS
C407
Substituted Anilines
Column:
P/N: Carrier: Oven:
DB-5ms
30 m x 0.25 mm id, 0.5 m 122-5536 Helium at 33.3 cm/sec, measured at 150C 40C for 5 min 40-290C at 12/min 290C for 10 min Splitless, 250C 30 sec purge activation time 1 L of 25 ng/L standard MSD, 325C transfer line
Injector:
Detector:
Compound list for all chromatograms 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. 18. Aniline 2-Chloroaniline 2,6-Dimethylaniline 3-Chloroaniline 4-Chloroaniline 4-Bromoaniline 2-Nitroaniline 3,4-Dichloroaniline 3-Nitroaniline 2,4,5-Trichloroaniline 4-Chloro-2-nitroaniline 4-Nitroaniline 2-Chloro-4-nitroaniline 2,6-Dichloro-4-nitroaniline 2-Chloro-4,6-dinitroaniline 2,6-Dibromo-4-nitroaniline 2,4-Dinitroaniline 2-Bromo-4,6-dinitroaniline (m/z) (93) (127) (121) (127) (127) (171) (138) (161) (65) (195) (172) (138) (172) (176) (217) (266) (183) (261)
C408
411

Phenols
Column: HP 5MS
P/N: Carrier: Oven: Injection: Sample: Purge Time: Detector: 30 m x 0.25 mm id, 0.25 m 19091-5433 Helium, 33 cm/sec, constant flow 35C for 5 min 35-220C at 8C/min 1 L, Splitless, 250C 20 g/mL phenols in methylene chloride 0.75 min FID 300C 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. Phenol 2-Chlorophenol 2-Nitrophenol 2,4-Dimethylphenol 2,4-Dichlorophenol 4-Chloro-3-methylphenol 2,4,6-Trinitrophenol 2,4-Dinitrophenol 4-Nitrophenol 2-Methyl-4,6-dinitrophenol Pentachlorophenol
4 1 5 2 3
6 7 8 9 10 11
0 C832
10 Time (min)
15
20
25
GC and GC/MS
Phenols
Column:
DB-5ms
30 m x 0.25 mm id, 0.5 m 122-5536 Helium at 22 cm/sec, measured at 100C 100C for 1 min 100-270C at 10/min Split 1:50, 250C 1 L of 50 ng/L standard in toluene/p-xylene FID, 300C Nitrogen makeup gas at 30 mL/min
Detector:
1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16.
Phenol 2-Chlorophenol o-Cresol m-Cresol p-Cresol 2,6-Xylenol 2,4-Xylenol 2,5-Xylenol 2-Nitrophenol 3,5-Xylenol 2,3-Xylenol 2,4-Dichlorophenol 3,4-Xylenol 2,4,6-Trichlorophenol 2,4-Dinitrophenol 1-Naphthol
C400
412

Phenols
Column:
P/N: Carrier: Oven: Injector: Detector: 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 2,6-Xylenol 2-Cresol Phenol 2-Ethylphenol 2,5-Xylenol 4-Cresol 2,4-Xylenol 3-Cresol 2-Isopropylphenol 2,3-Xylenol 3,5-Xylenol 4-Ethylphenol 3,4-Xylenol 2,3,5-Trimethylphenol
DB-WAX
30 m x 0.25 mm id, 0.25 m 122-7032 Hydrogen at 43 cm/sec 165C isothermal Split 1:50, 1 L, 250C FID, 300C Nitrogen makeup gas at 30 mL/min
GC and GC/MS
C117
Halocarbons
Column:
P/N: Carrier: Oven:
GS-GasPro
30 m x 0.32 mm id 113-4332 Helium at 30 cm/sec 130C for 4 min 130-225C at 10/min 225C Hold Split 1:67, 1 L, 250C FID, 250C
Injector: Detector: 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. 18. 19. 20. 21. 22.
CH4 CHCIF2 (Freon 22) CCI2F2 (Freon 12) CICF2 CF2 Cl (Freon 114) CHCI2 F (Freon 21) CCI3F (Freon 11) CF2Br2 (Freon 12B2) CH3I CH2CI2 trans-CICH=CHCI CF3CCl3 (Freon 113) cis-CICH=CHCI CHCI3 ? from CCI4 CCI4 ? from CCI4 CH3CH2I CH2Br2 CHCI2Br C4F9I CHCIBr2 CH3CH2CH2I
C532
413

Ethylene Oxide
(Courtesy of J. Chromatogr. Sci., 28:97 [1990])
Halothane
Column:
GS-GasPro
15 m x 0.32 mm 113-4312 Helium at 45 cm/sec 240C, isothermal Split 1:100, 0.2 L, 200C FID, 200C
Column:
DB-WAX
122-7032 Helium at 1 mL/min 60C for 2 min 60-180C at 16/min Split 1:50, 250C FID, 300C Nitrogen makeup gas at 30 mL/min
1. Ethylene oxide 2. 2-Chloroethanol 3. Ethylene glycol (solvent: Dimethylformamide)
1. 2. 3. 4.
Nitrogen Halothane Diethyl ether Acetone
GC and GC/MS
C116
C591
Inorganic Gases
Column:
P/N:
Inorganic Hydride Gases

Column: HP-1
Carrier: Oven: Helium at 53 cm/sec 25C for 3 min 25-200C at 10/min 200C Hold Split 1:50, 200C, 50 L TCD, 250C P/N: Carrier: Oven: Injection: Detector: 50 m x 0.2 mm id, 0.5 m 19091Z-205 Helium, 35 cm/sec A: -70C isothermal B: 30C isothermal Split (25:1), 1 L FPD (535 m filter) 1. Arsine 2. Phosphine 3. Selenide 4. Diborane 5. Tetraborane 6. Pentaborane 7. Dihydropentaborane 0.1% 0.1% 0.1% 0.10 ppm 0.10 ppm 0.10 ppm 0.60 ppm
GS-GasPro
30 m x 0.32 mm id 113-4332
Injector: Detector: 1. 2. 3. 4. 5. 6. 7.
Nitrogen CO2 SF6 COS H2S Ethylene oxide SO2
(A) -70C
(B) 30C
5 6 3 7
C531
C828
414

Life Sciences
Life Sciences
Common Drug Screen
Column:
P/N:
DB-5
30 m x 0.25 mm id, 0.25 m 122-5032
Peak 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. 18. 19. 20. 21. 22. 23. 24. 25. 26. 27. 28. 29. 30. 31. 32. 33. 34.
Drug Nicotine Phenmetrazine Ibuprofen Procaine Allobarbital Aprobarbital Butabarbital Secobarbital Pentobarbital Phenacetin Amobarbital Benzphetamine Acetaminophen Hydroxyphenamate Dimenhydrinate Meprobamate Benactyzine Hexobarbital Doxylamine Caffeine Chlorpheniramine Methapyrilene Thenyldiamine Phenobarbital Bromopheniramine Chlorcyclizine Cocaine Pyrrobutamine Codeine Diazepam Morphine Hydrocodone Oxymorphone Heroin
DB-17 Time 9.87 11.80 12.06 13.48 13.91 14.14 14.56 14.87 15.41 15.72 15.87 16.14 16.34 16.47 16.93 17.12 17.26 17.52 17.69 18.05 18.47 18.72 18.87 19.11 19.71 20.75 21.32 22.79 24.27 25.27 25.36 25.98 28.27 29.32
DB-5 Time 8.57 9.95 10.64 14.82 12.02 12.27 12.76 14.31 13.73 12.94 13.43 14.96 11.12 15.31 13.79 14.44 14.71 15.22 15.87 13.11 16.35 16.68 16.85 16.29 17.39 19.13 18.88 20.89 20.66 21.13 21.12 21.26 22.21 23.14
Column:
P/N: Carrier: Oven:
DB-17
30 m x 0.25 mm id, 0.25 m 122-1732 Hydrogen at 41 cm/sec, measured at 80C 80C for 1 min 80-280C at 10/min 280C for 9 min Split 1:40, 250C FID, 300C
Injector: Detector:
GC and GC/MS
DB-5
DB-17
C131
415

Life Sciences
Drug Screen
Column:
P/N: Carrier: Oven:
DB-1ms
30 m x 0.25 mm id, 0.25 m 122-0132 Helium at 40 cm/sec, measured at 50C 50C for 1.0 min 50-125C at 25/min 125-325C at 10/min 325C for 5 min Cold Splitless using Optic II injector 50-250C at 10/sec, 45 sec purge activation time 1 L injection of 50-150 ppm standard FID, 300C
1. 2. 3. 4. 5. 6. 7. 8. 9.
Nicotine Caffeine Glutethimide Lidocaine PCP Phenobarbital Methadone primary metabolite Methaqualone Methadone
10. 11. 12. 13. 14. 15. 16. 17. 18.
Cocaine Desipramine Carbazepine Trimipramine Heroin Fentanyl Ibogaine Triazolam LSD
Injector:
Sample: Detector:
C759
GC and GC/MS
Urine Drug Screen

Column:
P/N: JCarrier: Injector: Oven: Detector:
Amphetamines and Precursors - TMS Derivatives

Column:
P/N: Carrier: Oven: Injector: Detector: 1. 2. 3. 4. 5. 6. 7. 8.
Ultra 2
50 m x 0.32 mm id, 0.52 m 19091B-115 Hydrogen 80 cm/sec Splitless 45C for 1.5 min 45-300C at 6C/min FID
DB-5
20 m x 0.18 mm id, 0.4 m 121-5023 Helium at 39 cm/sec, measured at 100C 100-240C at 10/min Split 1:100, 250C 1 L of 2 g/L each in pyridine FID, 300C Nitrogen makeup gas at 30 mL/min 9. 10. 11. 12. 13. 14. 15. 16. Phenacetin 3,4-Methylenedioxyamphetamine (MDA) 3,4-Methylenedioxymethylamphetamine 4-Methyl-2,5-dimethoxyamphetamine (STP) Phenyl ephedrine 3,4-Methylenedioxyethylamphetamine (MDE; Eve) Caffeine Benzphetamine
12
1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13.
Amphetamine Methamphetamine Meperidine Phencyclidine (PCP) Methadone Proposyphene Amitriptyline Cocaine Imipramine Cyheptamide (ISTD) Codeine Diazepam Flurazepam
Phenylacetone Dimethylamphetamine Amphetamine Phentermine Methamphetamine Methyl ephedrine Nicotinamine Ephedrine
8 6 7
10
13 11 12
12 min
C100B
C123
416

Life Sciences
Anesthetics
Column:
P/N: Carrier: Oven:
Anticonvulsants
Column:
P/N: Carrier: Oven: Injector: Detector: 1. 2. 3. 4. 5.
DB-5ms EVDX
25 m x 0.20 mm id, 0.33 m 128-8522 Helium at 35 cm/sec, measured at 55C 55C for 1 min 55-130C at 25/min 130-325C at 15/min Splitless, 250C 45 sec purge activation time 1 L of 50-100 ng/L standard in methanol MSD, 280C transfer line full scan at m/z 35-400
DB-1
30 m x 0.53 mm id, 1.5 m 125-1032 Helium at 8 mL/min 160C for 2 min 160-275C at 15/min Megabore Direct, 250C 1 L of 100 ng/L in methanol FID, 300C Nitrogen makeup gas at 30 mL/min 6. 7. 8. 9. 10. Phenobarbital Primidone Carbamazepine Phenytoin 5-Methyl-5-phenylhydantoin
Injector:
Detector:
1. 2. 3. 4. 5. 6. 7. 8. 9.
Salicylamide Benzocaine Lidocaine Procaine Nefopam Mepivacaine Tetracaine Butacaine Dibucaine
Ethosuximide Methsuximide Phensuximide N-Desmethyl methsuximide Phenylethylmalonamide
C216
C141
GC and GC/MS
Antihistamines
Column:
P/N: Carrier: Oven:
DB-5
30 m x 0.32 mm id, 0.25 m 123-5032 Helium at 40 cm/sec, measured at 55C 55C for 1 min 55-175C at 30/min 175-320C at 10/min 320C for 1 min Splitless, 250C 30 sec purge activation time 1 L of 50 ng/L each in methanol FID, 300C Nitrogen makeup gas at 30 mL/min 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. Pheniramine Dimenhydrinate Diphenhydramine Doxylamine Phenyltoloxamine Tripelennamine Methapyrilene Chlorpheniramine Cyclizine Carbinoxamine Diphenylpyraline Bromopheniramine 13. 14. 15. 16. 17. 18. 19. 20. 21. 22. 23. Thonzylamine Chlorcyclizine Pyrilamine Triprolidine Promethazine Antazoline Clemizole Hydroxyzine Meclizine Cinnanzine Buclizine
Injector:
Detector:
C138
417

Life Sciences
Antiepileptic Drugs
Column:
P/N: Carrier: Injection: Oven: Detector:
Tricyclic Antipsychotics
Column:
P/N: Carrier: Oven:
Ultra 2
12 m x 0.32 mm id, 0.17 m 19091B-012 Helium, 14 psi Split, 35:1 100-230C at 15C/min NPD 1. 2. 3. 4. 5. 6. 7. 8. Ethotoin PEMA n-Des Phenobarbital Carbepoxide 10/11 Primidone Phenyltoin ISTD
Ultra 2
12 m x 0.2 mm id, 0.33 m 19091B-101 Hydrogen, 106 cm/sec 250C for 3 min 250-290C at 10C/min 290C for 10 min Split, 75:1 FPD 5954-7614 4 3
Injector: Detector: Pub No.:
1 2
2 1
1. 2. 3. 4. 5.
Triflupromazine Promethazine Promazine Chlorpothixene Thioridazine

5
5 3 7
C1012
GC and GC/MS
C1010
Barbiturates
Column:
P/N:
DB-35ms
30 m x 0.25 mm id, 0.25 m 122-3832
Carrier: Oven:
Injector: Detector:
Helium at 31 cm/sec, measured at 50C 50C for 0.5 min 50-150C at 25/min 150-300C at 10/min Splitless, 250C 30 sec purge activation time MSD, 280C transfer line full scan at m/z 40-270
1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. 18.
Barbital Allobarbital Aprobarbital Butabarbital Butethal Butalbital Amobarbital Talbutal Pentobarbital Methohexital Secobarbital Hexobarbital Thiopental Cyclopentylbarbital Mephobarbital Thiamylal Phenobarbital Alphenal
C572
418

Life Sciences
Benzodiazepines
Column:
P/N: Carrier: Oven:
DB-35ms
30 m x 0.25 mm id, 0.25 m 122-3832 Helium at 31 cm/sec, measured at 50C 50C for 0.5 min 50-150C at 25/min 150-340C at 10/min 340C for 6 min Splitless, 250C 30 sec purge activation time MSD, 280C transfer line full scan at m/z 40-400
Injector: Detector:
1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17.
Medazepam Halazepam Oxazepam Lorazepam Diazepam Demoxepam Desmethyldiazepam Clobazam Temazepam Flunitrazepam Delorazepam Bromazepam Prazepam Flurazepam Clonazepam Alprazolam Triazolam
C574
GC and GC/MS
Fentanyls
Column:
Tocopherols
DB-1701
30 m x 0.53 mm id, 1.0 m 125-0732 Hydrogen at 15 mL/min 270C isothermal Split 1:5, 0.8 L, 250C FID, 300C Nitrogen makeup gas at 30 mL/min
Column:
P/N: Carrier: Oven:
DB-17ms
30 m x 0.25 mm id, 0.25 m 122-4732 Helium at 40 cm/sec, measured at 150C 300C for 1 min 300-320C at 25/min 320C for 4 min Split 1:25, 310C 1 L of 1-10 ng/L in isooctane MSD, 310C transfer line full scan at m/z 45-550
Injector: 1. 2. 3. 4. 5. Fentanyl Sufentanyl Carfentanyl Lofentanyl Alfentanyl Detector:
1. 2. 3. 4.
-Tocopherol -Tocopherol -Tocopherol -Tocopherol
C129
C474
419

Life Sciences
Hallucinogens
Column:
P/N: Carrier: Oven: Helium at 30 cm/sec, measured at 50C 50C for 0.5 min 50-125C at 25/min 125-255C at 10/min 255-320C at 25/min 320C for 16 min Splitless, 250C 30 sec purge activation time 1 L of 10-50 ng/L standard in methanol MSD, 300C transfer line full scan at m/z 40-350 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 4-Methylaminorex Mescaline Dimethyltryptamine Ketimine TCP (Tenocyclidine) PCP Diethyltryptamine Bufotenine Fentanyl Ibogaine LSD Psilocin (peak not shown)
DB-17ms
30 m x 0.25 mm id, 0.25 m 122-4732
Injector:
Detector:
C468
GC and GC/MS
Sedative Hypnotics
Column:
P/N: Carrier: Oven:
Narcotics
Column:
P/N: Carrier: Oven:
DB-5ms EVDX
12 m x 0.20 mm id, 0.33 m 128-8512 Helium at 35 cm/sec, measured at 55C 55C for 1 min 55-130C at 25/min 130-325C at 15/min 325C for 4 min Splitless, 250C 45 sec purge activation time 1 L of 50-100 ng/L standard in methanol MSD, 280C transfer line full scan at m/z 35-400
DB-5ms
30 m x 0.25 mm id, 0.25 m 122-5532 Helium at 31 cm/sec, measured at 50C 50C for 0.5 min 50-150C at 25/min 150-325C at 10/min Splitless, 250C 30 sec purge activation time MSD, 300C transfer line full scan at m/z 40-380 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. Dextromethorphan Codeine Dihydrocodeine Norcodeine Ethylmorphine Morphine Normorphine 6-Acetylcodeine 6-Monoacetylmorphine Heroin
Injector: Detector
Injector:
Detector: 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. Ethchlorvynol Ethinamate Pyrithyldione Talbutal Meprobamate Glutethimide Methaqualone Propiomazine Haloperidol Sulpiride Droperidol
2,3
5 9 4 6 7
10
C218
C424
420

Life Sciences
Narcotics and Adulterants
Column:
Over-the-Counter Pain Killers - TMS Derivatives

Column:
P/N: Carrier: Oven: Injector: Detector: 1. 2. 3. 4. 5. 6. 7.
DB-5
30 m x 0.32 mm id, 0.25 m 123-5032 Helium at 40 cm/sec, measured at 140C 140-320C at 12/min 320C for 4 min Split 1:75, 250C 1 L of 0.5 g/L each in methanol FID, 300C Nitrogen makeup gas at 30 mL/min 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11.
DB-5
20 m x 0.18 mm id, 0.4 m 121-50232 Helium at 39 cm/sec, measured at 100C 100-240C at 10/min Split 1:100, 250C 1 L of 2 g/L each in pyridine FID, 300C Nitrogen makeup gas at 30 mL/min Nicotine Unknown Acetylsalicylic acid (aspirin) Ibuprofen Acetaminophen Unknown Caffeine
Caffeine Ketamine Lidocaine Procaine Cocaine Codeine Morphine 6-Acetylcodeine Diacetylmorphone (Heroin) Quinine Strychnine
C135
C140
GC and GC/MS
Aspirin and Ibuprofen in Methanol

Column:
P/N: Carrier: Oven: Injector: Detector: 1. Aspirin 2. Ibuprofen
Free Steroids
Column:
P/N: Carrier: Oven:
DB-FFAP
30 m x 0.25 mm id, 0.25 m 122-3232 Hydrogen at 24 cm/sec, measured at 180C 180C isothermal Split 1:50, 250C FID, 300C Nitrogen makeup gas at 30 mL/min
DB-17
30 m x 0.25 mm id, 0.15 m 122-1731 Hydrogen at 44 cm/sec 260C isothermal Injector: Detector: Split 1:100, 1 L, 250C FID, 300C Nitrogen makeup gas at 30 mL/min
1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12.
Coprostane (5--cholestane) 5--Androsterone 5--Cholestane Androsterone Epiandrosterone (trans-androsterone) 17--Estradiol -Estradiol Estrone Progesterone Cholesterol Estriol Stigmasterol
C139
C148
421

Life Sciences
Anabolic Steroids
Column:
P/N: Carrier: Oven: Injector: Detector: 1. 2. 3. 4. 5. 6. 7. 8.
DB-1
30 m x 0.25 mm id, 0.1 m 122-1031 Helium at 40 cm/sec, measured at 180C 180-320C at 10/min 320C for 4 min Split 1:40, 2 L of 0.125 g/L each in methanol FID, Nitrogen makeup gas at 30 mL/min 11. 12. 13. 14. 15. 16. 17. Oxymetholone 19-Nortestosterone-17-propionate 4-Chlortestosterone-17-acetate (Clostebal) Stanozolol 1-Dehydrotestosterone benzoate 19-Nortestosterone-17-decanoate 1-Dehydrotestosterone undecylenate
9-THC (Marijuana) and Major Metabolites - TMS Derivatives

Column:
P/N: Carrier: Oven:
DB-5
30 m x 0.32 mm id, 0.25 m 123-5032 Helium at 40 cm/sec, measured at 100C 100C for 1 min 100-175C at 30/min 175-295C at 12/min Splitless, 250C 30 sec purge activation time 1 L of 0.1 g/L each in pyridine FID, 300C Nitrogen makeup gas at 30 mL/min
Dehydroisoandrosterone (Prasterone) 5-Androstan-17-ol-3-one (Stanolone) 19-Nortestosterone (Nandrolone) Mesterolone Testosterone 1-Dehydrotestosterone (Boldenone) 17-Methyltestosterone 1-Dehydro-17--methyltestosterone (Methandrostenolone) 9. Norethandrolone 10. 1-Dehydrotestosterone acetate
Injector:
Detector:
1. 9-THC 2. L-11-Hydroxy-9-THC 3. L-9-Carboxy-11-nor-9-THC
C147
C136
GC and GC/MS
Blood Alcohols (Static Headspace/Split)

Column:
Blood Alcohols (Static Headspace/Split)

Column:
DB-ALC1
30 m x 0.53 mm id, 3.0 m 125-9134 Helium at 80 cm/sec, measured at 40C 40C Isothermal Headspace to split inlet, Split 1:10, 250C FID, 300C Nitrogen makeup gas at 23 mL/min Headspace Conditions Oven: Loop: Transfer Line: Vial Equil. Time: Pressurization Time: Loop Fill Time: Loop Equil. Time: Inject Time: Sample Loop Size: Sample Composition: 70C 80C 90C 10 min 0.20 min 0.20 min 0.05 min 0.1 - 0.2 min 1.0 mL 0.1% Ethanol, 0.001% Others
DB-ALC2
30 m x 0.53 mm id, 2.0 m 125-9234 Helium at 80 cm/sec, measured at 40C 40C Isothermal Headspace to split inlet, Split 1:10, 250C FID, 300C Nitrogen makeup gas at 23 mL/min
3
1. 2. 3. 4. 5. 6.
Methanol Acetaldehyde Ethanol Isopropanol Acetone 1-Propanol
1. 2. 3. 4. 5. 6.
Methanol Acetaldehyde Ethanol Isopropanol Acetone 1-Propanol
Headspace Conditions Oven: Loop: Transfer Line: Vial Equil. Time: Pressurization Time: Loop Fill Time: Loop Equil. Time: Inject Time: Sample Loop Size: Sample Composition:
70C 80C 90C 10 min 0.20 min 0.20 min 0.05 min 0.1 - 0.2 min 1.0 mL 0.1% Ethanol, 0.001% Others
1 2
0.5
1.5 Time (min)
2.5
3.0
C670
C671
422

Life Sciences
Blood Pollutants
Column:
DB-ALC1
30 m x 0.53 mm id, x 3.0 m 125-9134 Helium, 36 cm/sec, measured at 40C 40C for 5 min 40-210C at 10/min 250C, Split 1:10 FID, 300C
1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16.
Methanol* Acetaldehyde* Ethanol* Diethyl ether Isopropyl alcohol* Methylene Chloride Acetone* Acetonitrile Ethyl formate t-Butyl alcohol* 1-Propanol MTBE Hexane Chloroform sec-Butyl alcohol 2-Chlorobutane
17. 18. 19. 20. 21. 22. 23. 24. 25. 26. 27. 28. 29. 30. 31. 32.
MEK (2-Butanone) Ethyl acetate 1,1-Trichloroethane Carbon tetrachloride 1-Chlorobutane Benzene 1-Butanol Heptane Ethylene glycol soamyl alcohol Toluene Isopropyl amine (not shown) Ethylbenzene m,p-Xylene o-Xylene DMSO
C1072
GC and GC/MS
Blood Pollutants
Column:
DB-ALC2
30 m x 0.53 mm id, x 2.0 m 125-9234 Helium, 36 cm/sec, measured at 40C 40C for 5 min 40-210C at 10/min 250C, Split 1:10 FID, 300C
1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16.
Methanol* Acetaldehyde* Ethanol* Diethyl ether Isopropyl alcohol* Methylene Chloride Acetone* Acetonitrile Ethyl formate t-Butyl alcohol* 1-Propanol MTBE Hexane Chloroform sec-Butyl alcohol 2-Chlorobutane
17. 18. 19. 20. 21. 22. 23. 24. 25. 26. 27. 28. 29. 30. 31. 32.
MEK (2-Butanone) Ethyl acetate 1,1-Trichloroethane Carbon tetrachloride 1-Chlorobutane Benzene 1-Butanol Heptane Ethylene glycol soamyl alcohol Toluene Isopropyl amine (not shown) Ethylbenzene m,p-Xylene o-Xylene DMSO
C1073
423

Life Sciences
Residual Solvents, USP <467>
Column:
DB-624
30 m x 0.53 mm id, 3.0 m 125-1334 Helium at 35 cm/sec, measured at 40C 40C for 20 min 40-90C at 5/min Megabore Direct, 250C 5 m phenylmethylsilane deactivated retention gap FID, 300C Nitrogen makeup gas at 30 mL/min
1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12.
Methanol Ethanol Ethyl ether Acetone Isopropanol Acetonitrile Methylene chloride tert-Butanol n-Hexane n-Propanol Methyl ethyl ketone (MEK) Ethyl acetate
13. 14. 15. 16. 17. 18. 19. 20. 21. 22. 23. 24.
Tetrahydrofuran (THF) Chloroform sec-Butanol Cyclohexane Benzene n-Heptane Trichloroethylene n-Butanol 1,4-Dioxane Pyridine Toluene Dimethylformamide (DMF)
C144
GC and GC/MS
Fast GC Analysis of USP Solvents

Column:

HP-Fast GC Residual Solvent Column

30 m x .53 mm id, 1.0 m 19095V-420 6890 Helium, 30 cm/min, constant flow 40C for 1.7 min 40-90C at 6C/min 90-250C at 30C min Headspace, 180C, Split 7/1 FID, 260C
Injector: Detector:
1. 2. 3. 4. 5.
3
Methylene Chloride (25 ppm) Chloroform (12 ppm) Benzene (25 ppm) Trichloroethylene (25 ppm) 1, 4-Dioxane (25 ppm)
4 2

6
4 Time (min)
C843
424

Petroleum
Petroleum
Noble Gases
Column:
Permanent Gases
Column:
P/N: GC: Carrier: Oven: Sample: Detector:
HP-PLOT/MoleSieve
30 m x 0.53 mm i.d., x 50 m 19095P-MS0 6890 Helium, 4 mL/min 35C for 3 min 35-120C at 25C/min 120C for 5 min 250 L, split (50:1) TCD 4 2 5 6
HP-PLOT/MoleSieve
30 m x 0.32 mm i.d., 12 m 19091P-MS4 6890 Helium, 2 mL/min 40C Isothermal 250 L, split (75:1) TCD
Sample: Detector:
1. 2. 3. 4. 5. 6.
Neon Argon Oxygen Nitrogen Krypton Xenon
1. 2. 3. 4. 5. 6.
Neon Argon Oxygen Nitrogen Methane Carbon monoxide
5 3 1 2
GC and GC/MS
2 Time (min) C887
C886
4 Time (min)
Baseline Resolution of Air/CO, CO2, and Methane in a Natural Gas Sample

Column:
Natural Gas
Column:
P/N: Carrier: Oven: 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. Air/CO CO2 Ethane H2S Water C3 i-C4/n-C4 neo-C5 i-C5/n-C5 C6 C7 Injection: Sample: Detector:
HP-PLOT Q
30 m x 0.53 mm i.d., 40 m 19095P-Q04 6890 Helium (8.6 mL/min @ 60C) 60C for 2 min 60-240C at 30C/min 240C for 1 min Split mode (12:1) 0.25 cc natural gas sample, Methane, 80%+ TCD 250C
HP-PLOT/Al2O3 S
15 m x 0.53 mm i.d., 15 m 19095P-S21 Helium, 50 cm/sec (100C), 6 mL/min 100C for 1.5 min 100-180C at 30C/min Split (50:1), 5 L, Inlet 250C Natural gas P/N 5080-8756 FID 250C
1. 2. 3. 4. 5. 6. 7. 8.
Methane Ethane Propane iso-Butane n-Butane iso-Pentane n-Pentane n-Hexane
1 23
45
1 2
6 5 67 8 4 8 9 10 11 0
C860
4 Time (min)
8
C859
2 Time (min)
425

Petroleum
Ethylene
Column:
P/N: GC: Sample: Carrier: Oven: Injection: Detector: 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. 18. 19. 20. 21. 22. 23. Methane Ethane Ethylene Propane Cyclopropane Propylene Isobutane n-Butane Propadiene Acetylene trans-2-Butene Butene-1 Isobutylene cis-2-Butene Isopentane n-Pentane 1,3-Butadiene Propyne trans-2-Pentene 2-Methyl-2-butene Pentene-1 cis-2-Pentene n-Hexane
HP- PLOT/Al2O3 S
50 m x 0. 53 mm i.d., 15 m 19095P-S25 6890 Ethylene 98.4% Helium, 50 cm/sec (35C), 7 mL/min constant flow 35C for 2 min 35-100C at 5C/min Split 65:1, 250C, 5 L FID 250C
1 2
11 12 14 16
17
13 15 18 19 20 10 9 21 22 23 10 Time (min) 15 20 25
GC and GC/MS
5 0
C885
Impurities in Ethylene
Column:
P/N: Carrier: Oven:
GS-Alumina/KCl
50 m x 0.53 mm i.d. 115-3352 Helium at 8 mL/min, measured at 35C 35C for 2 min 35-190C at 10/min 190C for 3 min Split 1:40, 200C 0.2 mL of trace hydrocarbons in ethylene FID, 200C Nitrogen makeup gas at 20 mL/min
Injector:
Detector:
1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. 18. 19.
Methane Ethane Ethylene Propane Cyclopropane Propylene Isobutane Acetylene n-Butane Propadiene trans-2-Butene 1-Butene Isobutylene cis-2-Butene Isopentane n-Pentane Propyne 1,3-Butadiene 1-Pentene
C479
426

Petroleum
Impurities in Propylene
Column:
P/N: Carrier: Oven: 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. 18. 19. Methane Ethane Ethylene Propane Cyclopropane Propylene Isobutane Acetylene n-Butane Propadiene trans-2-Butene 1-Butene Isobutylene cis-2-Butene Isopentane n-Pentane Propyne 1,3-Butadiene 1-Pentene
GS-Alumina/KCl
50 m x 0.53 mm i.d. 115-3552 Helium at 10 mL/min, measured at 35C 35C for 2 min 35-190C at 10/min 190C for 3 min Split 1:30, 200C 0.2 mL of trace hydrocarbons in propylene FID, 200C Nitrogen makeup gas at 20 mL/min
Injector:
Detector:
C480
GC and GC/MS
Propylene
Column:
P/N: Carrier: Oven:
GS-Alumina
50 m x 0.53 mm i.d. 115-3552 Helium at 10 mL/min, measured at 35C 35C for 2 min 35-190C at 10/min 190C for 3 min Split 1:30, 200C 0.2 mL of trace hydrocarbons in propylene FID, 200C Nitrogen makeup gas at 20 mL/min
Injector:
Detector:
1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. 18. 19. 20.
Methane Ethane Ethylene Propane Cyclopropane Propylene Isobutane Acetylene n-Butane Propadiene trans-2-Butene 1-Butene Isobutylene cis-2-Butene Isopentane n-Pentane Propyne 1,3-Butadiene 1-Pentene n-Hexane
C586
427

Petroleum
Propylene, Butene-1 and Ethylene
Column:
P/N: Carrier: Oven: Injector: Detector: Propylene 1. Methane 2. Ethylene 3. Propylene 4. Propane
DB-Petro100
100 m x 0.25 mm i.d., 0.5 m 122-10A6 Helium at 28.5 cm/sec -40C for 20 min -40-120C at 5/min Split 1:16, 100 L, 200C FID, 250C Nitrogen makeup gas at 30 mL/min
Butene-1 1. Isobutane 2. Butene-1 3. 1,3-Butadiene 4. n-Butane 5. trans-2-Butene 6. cis-2-Butene
Ethylene 1. Methane 2. Ethylene 3. Ethane 4. Propylene
C154
1,3-Butadiene
GC and GC/MS
Column:
P/N: Carrier: Oven:
DB-624
25 m x 0.20 mm i.d., 1.12 m 128-1324 Helium at 1.0 mL/min -20C for 3 min -20 - 20C at 4/min 20 - 200C at 8/min 200C for 10 min Split 1:150, 250C 0.5 L injection FID, 250C
Injector: Detector:
Agilent Technologies wishes to thank DCG Industries (Pearland, TX) for providing this chromatogram. 8,9 11 12 2,3 13
7 10
Refined Butadiene Standard Component 1. Acetylene 2. Propane 3. Propylene 4. Propadiene (allene) 5. Propyne (methylacetylene) 6. Cyclopropane 7. Isobutane 8. Butene-1 9. Isobutylene 10. n-Butane 11. 1,3-Butadiene 12. trans-2-Butene 13. cis-2-Butene 14. 1-Butyne (ethylacetylene) 15. 1,2-Butadiene 16. 3-Methyl-1-butene 17. Isopentane 18. Pentene-1 19. n-Pentane 20. 2-Butyne (dimethylacetylene) 21. trans-2-Pentene 22. Isoprene 23. cis-2-Pentene 24. trans-1,3-Pentadiene 25. cis-1,3-Pentadiene 26. Benzene 27. Toluene 28. Dimer (4-vinylcyclohexene-1)
Gravimetric concentration (PPM) 20.7 19.8 296 21.1 21.0 20.0 506 999 495 494 balance 442 1946 20.2 28.9 19.8 50.1 29.8 50.1 150 5.57 20.0 13.9 13.8 7.73 20.3 20.2
28
20
5 4
6 14
5 5 75
15 16
17
19 18
10 10
22 21 23
12 5
24
26 25
15 15 17 5 20 20
27
0 C777
25
22 5
Time (min)
428

Petroleum
1,3-Butadiene Purity
Column:
P/N: Carrier: Oven: Refined Butadiene Standard Component Gravimetric concentration (PPM) 1. Acetylene 20.7 2. Propane 19.8 3. Propylene 296 4. Propadiene (allene) 21.1 5. Propyne (methylacetylene) 21.0 6. Cyclopropane 20.0 7. Isobutane 506 8. Butene-1 999 9. Isobutylene 495 10. n-Butane 494 11. 1,3-Butadiene balance 12. trans-2-Butene 442 13. cis-2-Butene 1946 14. 1-Butyne (ethylacetylene) 20.2 15. 1,2-Butadiene 28.9 16. 3-Methyl-1-butene 19.8 17. Isopentane 50.1 18. Pentene-1 29.8 19. n-Pentane 50.1 20. 2-Butyne (dimethylacetylene) 150 21. trans-2-Pentene 5.57 22. Isoprene 20.0 23. cis-2-Pentene 13.9 24. trans-1,3-Pentadiene 13.8 25. cis-1,3-Pentadiene 7.73 26. Benzene 20.3 27. Toluene 20.2 28. Dimer (4-vinylcyclohexene-1) 5 18
GS-Alumina
50 m x 0.53 mm i.d. 115-3552 Helium, 6.0 mL/min (constant flow mode) 45C for 3 min 6/min to 195C 195C for 15 min 250C, Split 1:50 0.5 L injection FID, 250C
Injector: Detector:
12
13 11
15,19
17
GC and GC/MS
23 16 21
13
14
15
16 Time (min)
17
18
19
20 28
3 7 10
20
14 2 6 4 1 n-C6 24 25 22 26 27
5 C798
10
15
20 Time (min)
25
30
35
429

Petroleum
Extended Hydrocarbon Analysis on GS-Alumina and GS-GasPro
Column: GS-Alumina
30 m x 0.53 mm i.d. P/N: 115-3532 Carrier: Helium at 52 cm/sec (6.7 mL/min), measured at 100C Oven: 100C for 1 min 100-140C at 8/min 140C for 0.5 min 140-200C at 30/min Injector: Split 1:8, 250C 300 L injection of 100 ppm V SUMMA cannister mixture Detector: FID, 275C Nitrogen makeup gas at 29 mL/min (not all peaks shown ) 1. Methane 2. Ethane 3. Ethylene 4. Propane 5. Propylene 6. Isobutane 7. Acetylene 8. n-Butane 9. trans-2-Butene 10. 1-Butene 11. cis-2-Butene 12. Cyclopentane 13. Isopentane 14. n-Pentane 15. Propyne 16. 1,3-Butadiene 17. Cyclopentene 18. 3-Methyl-1-butene 19. trans-2-Pentene 20. 2-Methyl-2-butene 21. 1-Pentene 22. cis-2-Pentene 23. Methylcyclopentane 24. 2,2-Dimethylbutane 25. Cyclohexane 26. 2,3-Dimethylbutane
Enlarged view of peaks 15-30
GC and GC/MS
C608
Column:
P/N: Carrier: Oven:
GS-GasPro
60 m x 0.32 mm i.d. 113-4362 Helium at 40 cm/sec (3.3 mL/min), measured at 80C 80C for 0.5 min 80-175C at 25/min 175C for 2 min 175-250C at 25/min Split 1:17, 250C 500 L injection of 100 ppmV SUMMA cannister mixture FID, 275C Nitrogen makeup gas at 32 mL/min
Enlarged view of peaks 18-40
Injector:
Detector:
27. 28. 29. 30. 31. 32. 33. 34. 35. 36. 37. 38. 39. 40. 41. 42. 43. 44. 45. 46. 47. 48. 49. 50. 51. 52. 53. 54. 55. 56. 57.
2-Methylpentane 3-Methylpentane Isoprene n-Hexane 4-Methyl-1-pentene trans-2-Hexene 2-Methyl-1-pentene cis-2-Hexene 2,4-Dimethylpentane Methylcyclohexane 2,3-Dimethylpentane 2-Methylhexane 3-Methylhexane n-Heptane Benzene Isooctane (2,2,4-trimethylpentane) 2,3,4-Trimethylpentane 3-Methylheptane 2-Methylheptane n-Octane Toluene n-Nonane Ethylbenzene m-Xylene p-Xylene o-Xylene Styrene Isopropylbenzene (cumene) n-Propylbenzene 1,3,5-Trimethylbenzene 1,2,4-Trimethylbenzene
430

Petroleum
Separation of Refinery Gas Sample
Column:
Refinery Gas
Column:
1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. Air/CO C1 CO2 Ethylene C2 H2O COS H2O Propylene C3 MeOH i-C4 t-C4 n-C4 cis-C4 i-C5 n-C5 P/N: GC: Carrier: Oven: Injection: Split: Detector:
HP-PLOT Q
0.53 mm x 30 m i.d., 40 m 19095P-Q04 6890 Helium p=9.0 psi @ 60C 60C for 5 min 60-200C at 20C/min 200C for 1 min 250C Split mode 0.25 cc valve Refinery gas and others 100 mL/min TCD 250C
HP-PLOT/AI203 S
50 m x 0.53 mm i.d., 15 m 19095P-S25 6890 Helium 7 mL/min 100C Isothermal Split (100:1), inlet 250C, 5 L 100 mL/min FID 250C 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. Methane Ethane Ethylene Propane Propylene iso-Butane n-Butane trans-2-Butene 1-Butene cis-2 Butene iso-Pentane n-Pentane
Inlet: Sample: Split flow: Detector:
1 23 5
2 6
13 14 11 12 4 10 6 7 8 9 16 17 3 5 15 1 4
9 8
10 11
GC and GC/MS
12 12
0
C862
6 Time (min)
10
12
0
C863
6 Time (min)
10
Refinery Gas
Column:
P/N: Carrier: Oven:
PONA
50 m x 0.2 mm i.d., 0.5 m 19091S-001 Helium, 1 mL/min 35C for 3 min 35-100C at 10C/min 100C for 5 min Split (200 -400:1), 2 cc loop FID 250C 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. C1 C2, C2= C3= C3 IC4 NC4 1-Butene/t-2-butene cis-2-Butene IC5 NC5
5 67 8
10
3
C864
4 Time (min)
431

Petroleum
Volatile Sulfur Compounds
Column:
P/N: Carrier: Oven: 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. Hydrogen sulfide Carbonyl sulfide Methyl mercaptan Ethyl mercaptan Dimethyl sulfide Carbon disulfide 2-Propanethiol 2-Methyl-2-propanethiol 1-Propanethiol Ethyl methyl sulfide Thiophene 2-Methyl-1-propanethiol 13. 14. 15. 16. 17. 18. 19. 20. 21. 22. 23. 24. Diethyl sulfide 1-Butanethiol Methyl disulfide 2-Methylthiophene 3-Methylthiophene Tetrahydrothiophene 1-Pentanethiol 2-Ethylthiophene 2,5-Dimethylthiophene 1-Hexanethiol Ethyl disulfide 1-Heptanethiol
DB-1
30 m x 0.32 mm i.d., 5.0 m 123-1035 Helium at 23 cm/sec (H2S at 50C) 50C for 4 min, 50-120C at 20/min, 120C for 4 min, 120-220C at 25/min, 220C for 2.5 min Split 1:10, 200C, 600 L of sulfur gas standard, 3 ppmV each component PFPD (OI Analytical), 220C
Injector: Detector:
Agilent wishes to thank Air Toxics, Ltd. (Folsom, CA) for providing the standard mixture shown in this chromatogram.
GC and GC/MS
C775

432

Petroleum
Sulfur Gas Analysis in Light Hydrocarbon Streams
Column:
P/N: Injector: Carrier: Oven: Detector: Sulfur compounds (PFPD) A. Carbonyl sulfide B. Hydrogen sulfide C. Sulfur dioxide D. Carbon disulfide E. Methyl mercaptan F. Ethyl mercaptan Hydrocarbons (FID) 1. Methane 2. Ethane 3. Ethylene 4. Acetylene 5. Propane 6. Propylene 7. iso-Butane 8. n-Butane 9. 1-Butene/Methyl acetylene 10. trans-2-Butene 11. 1,3-Butadiene 12. cis-2-Butene 13. iso-Pentane 14. n-Pentane
GS-GasPro
30 m x 0.32 mm i.d. 113-4332 200C, 1:20 split Helium, 10 psig, 2.0 mL/min @ 60C 60C for 2 min, 20/min to 260C and hold Two separate analyses under identical conditions on FID and PFPD
PFPD
Time (min)
10
FID
1
13 14 12
7 8 5 2
C800
10 11
GC and GC/MS
Sulfur Gas Analysis in Light Hydrocarbon Streams

Column:
P/N: Injector: Carrier: Oven: Detector:
GS-Q
30 m x 0.32 mm i.d. 113-3432 200C, 1:20 split Helium, 10 psig, 1.7 mL/min @ 100C 100C for 2 min, 20/min to 250C and hold Two separate analyses under identical conditions on FID and PFPD
Sulfur compounds (PFPD) A. Hydrogen sulfide B. Carbonyl sulfide C. Methyl mercaptan D. Ethyl mercaptan E. Carbon disulfide
PFPD
Hydrocarbons (FID) 1. Methane 2. Ethylene/Acetylene 3. Ethane 4. Propylene 5. Propane 6. Methyl acetylene 7. iso-Butane 8. 1-Butene 9. 1,3-Butadiene 10. n-Butane/cis-2-Butene 11. trans-2-Butene 12. iso-Pentane 13. n-Pentane
Time (min)
12
FID
4 1 5 6
7 13 11
9 10
C799
3 2
433

Petroleum
Sulfur Compounds in Propylene (1 ppm)
Column:
P/N: Oven: Injector: Sample: Detector: 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. COS H2 S Propylene CS2 Methyl mercaptan Ethyl mercaptan Thiophene Dimethyl sulfide 2-Propanethiol 1-Propanethiol 2-Methyl-2-propanethiol 2-Methyl-1-propanethiol 1-Methyl-1-propnaethiol 1-Butanethiol
Mercaptans
Column:
GS-GasPro
30 m x 0.32 mm i.d. 113-4332 60C for 2.5 minutes 60-250C at 10C/min OI Analytical Volatiles Inlet 5:1 split, 200 L gas sampling valve 1 ppm Sulfur compounds in Propylene OI Analytical Model 5380 PFPD
GS-GasPro
30 m x 0.32 mm i.d. 113-4332 Helium at 25 cm/sec 175C for 2 min 175-260C at 10/min Split, 80 mL/min split flow 0.2 mL 3 FID
1. 2. 3. 4. 5. 6. 7. 8. 9.
Ethyl mercaptan 2-Propyl mercaptan 1-Propyl mercaptan 2-Methyl-2-propyl mercaptan 2-Methyl-1propyl mercaptan 1-Methyl-1-propyl mercaptan 1-Butyl mercaptan 1-Pentyl mercaptan 1-Hexyl mercaptan
1 2
1 1 5 6 4 4 4
2 2
8 9
6 6 5 5
8 7 7 9 9
10 10
13 14 13
14
11 11 12 12 3 3
GC and GC/MS
C801 Chromatogram courtesy of OI Analytical.
Time (min)
0
C530
Sulfur Compounds in Natural Gas-Synthetic Mixture

Column:
Sulfur Compounds in Naphtha

Column:
P/N: Carrier: Oven:
HP-1
50 m x 0.2 mm i.d., 0.5 m 19091Z-205 Helium 35C for 10 min 35-300C at 7C/min Split (100:1) 0.5 mL FPD
3 12 45 7 8 9 11 12 10 13
1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16.
Hydrogen sulfide Methyl mercaptan Ethyl mercaptan Dimethyl sulfide Isopropyl mercaptan tert-Butyl mercaptan n-Propyl mercaptan Thiophene and sec-Butyl mercaptan Isobutyl mercaptan n-Butyl mercaptan tert-Amyl mercaptan Isoamyl mercaptan n-Amyl mercaptan n-Hexyl mercaptan tert-Dibutyl disulfide n-Octyl mercaptan
PONA
50 m x 0.2 mm i.d., 0.5 m 19091S-001 Helium, 26 cm/sec 35C for 15 min 35-70C at 8C/min 70-130C at 15C/min Split (400:1), 3 L FPD 1. 2. 3. 4. Thiophene Methyl thiophenes Ethyl and dimethyl thiophenes Benzothiophene
16
14 15 6
C870
30 min
C868
42 min
434

Petroleum
ASTM D16 Analytes- Aromatics Analysis
Column: HP-INNOWax
P/N: Carrier: Oven: 60 m x 0.32 mm i.d., 0.5 m 19091N-216 Helium at 20 psi, constant pressure mode 75C for 10 min 3C/min to 100C 10C/min to 145C 250C, Split/Splitless 100:1 to 400:1 split ratio 250C, FID Data aquisition rate at 20 Hz 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. Heptane Cyclohexane Octane Nonane Benzene Decane Toluene 1-4-Dioxane Undecane Ethylbenzene p-Xylene m-Xylene Cumene Dodecane 15. 16. 17. 18. 19. 20. 21. 22. 23. 24. 25. 26. 27. o-Xylene Propylebenzene p-Ethyltoluene m-Ethyltoluene tert-Butylbenzene sec-Butylbenzene Styrene Tridecane Diethylbenzene isomer Diethylbenzene isomer n-Butylbenzene -Methylstyrene Phenylacetylene
Injector: Detector:
1 23
6 7 25 9 10 11 12 13 14 15 16 18 17 19 20 21 22 23 27 26
GC and GC/MS
24
2.5
C806
7.5
10
12.5 Time (min)
15
17.5
20
22.5
Aromatics Analysis Ethylbenzene Impurities

Column:
P/N: Carrier: Oven:
HP-INNOWax
60 m x 0.32 mm i.d., 0.5 m 19091N-216 Helium at 20 psi, constant pressure mode 75C for 10 min 3C/min to 100C 10C/min to 145C 250C, Split/Splitless 100:1 to 400:1 split ratio 250C, FID 4 Data aquisition rate at 20 Hz
Injector: Detector:
1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14.
Benzene Toluene Undecane Ethylbenzene p-Xylene m-Xylene Isopropylbenzene o-Xylene n-Propylebenzene p-Ethyltoluene m-Ethyltoluene s-Butylbenzene Diethylbenzene Diethylbenzene
Impurities in p-Xylene-ASTM D3798

Column:
HP-INNOWax
60 m x 0.32 mm i.d., 0.5 m 19091N-216 6890 Helium, 32 cm/sec, 19.9 psi (60C), 2.5 mL/min constant flow 60C for 1 min 60-92C at 4C/min 92C for 4.5 min 92-220C at 20C/min 220C for 5 min Split (split ratio 100:1), 0.5 L 220C Neat, 99%+ FID 270C
1. 2. 3. 4. 5. 6.
Non aromatic hydrocarbon Toluene Ethylbenzene p-Xylene m-Xylene o-Xylene
1 2
5 4 6 7 8 3 9 11 10 13 1 14 2 0 5 Time (min) 10 15 12 5 3
6 10 11 Time (min) 12
5
C809
7.5
10
12.5 Time (min)
15
17.5
20
C897
435

Petroleum
Fast Styrene Analysis
Column:
Ethylene Oxide Synthetic Standard

1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. Nonaromatics Ethylbenzene p-Xylene m-Xylene Isopropylbenzene o-Xylene n-Propylbezene p/m-Ethyltoluene Stryene -Methylstyrene (366 ppm) Phenylacetylene (58 ppm) -Methylstyrene Benzaldehyde
DB-WAX
10 m x 0.10 mm i.d., 0.2 m 127-7013 6890 Helium 46.4 psi (48 cm/sec), constant flow, 0.5 mL/min 80C for 1.4 min 80-150C at 33.8C/min 150C for 0.5 min Split (300:1), 0.1 L FID
Column:
P/N:
HP-PLOT Q
30 m x 0.53 mm i.d., 40 m 19095P-Q04
1. Ethylene oxide 2. 2-Chloropropene
GC: Carrier: Oven: Injection: Detector:
6890 Helium, 25 psi 50C for 2 min 50-250C at 15C/min Split (40:1), 1 L liquid injection, sample 2000 ppm v/v FID 1 2
Injection: Dectector:
10
2 4 3 5 7 8 1 Time (min)
1. 2. 3. 4. 5. 6. 7. 8. Ethanol 2-Propanone Dichloromethane Acetic acid, methyl ester Diethyl ether Pentane Acetic acid, ethyl ester Hexane
6 11 12 13
GC and GC/MS
1 0
C992
0
C867
10 Time (min)
12
14
16
18
20
Oxygenates
Column:
P/N: GC: Oven: Carrier: Detector:
HP-PLOT Q
30 m x 0.32 mm i.d., 20 m 19095P-QO4 6890 150C for 2 min 150-250C at 15C/min Helium, 25 psig FID
Oxygenates in Gasoline ASTM D5599 (GC-OFID)

Column:
P/N: Carrier: Oven:
HP-1
60 m x 0.25 mm i.d., 1 m 19091Z-236 Helium 30 cm/sec constant flow 40C for 6 min 40-50C at 5C/min 50C for 4 min 50-175C at 25C/min 175C for 5 min Split (150:1), 0.5 L Wasson ECE OFID
2 3
1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15.
Water Methanol Ethanol 2-Propanol t-Butanol 1-Propanol MTBE sec-Butanol DIPE Isobutanol ETBE TAA 1,2-Dimethoxyethane 1-Butanol TAME
13
5 2 6 5 6 7 4 3 7 1 8 10 11 12 9 14 15 4
1
C854
4 Time (min)
0
C890
10 Time (min)
15
436

Petroleum
Denatured Fuel Ethanol-ASTM D5501
Column:
HP-1
100 m x 0.25 mm i.d., 0.5 m 19091Z-530 6890 Helium 24 cm/sec 15C for 12 min 15-250C at 19C/min 250C for 20 min Split, 200:1, 0.5 L FID 250C Nitrogen makeup gas at 30 mL/min
1. 2. 3. 4. 5. 6.
Methanol n-Butane Ethanol Isopentane n-Pentane Other hydrocarbons
3 5
6 1 2
GC and GC/MS
10
C892
20 Time (min)
Unleaded Gasoline
Column:
P/N: Carrier: Oven:
DB-Petro100
100 m x 0.25 mm i.d., 0.5 m 122-10A6 Helium at 25.6 cm/sec 0C for 15 min 0-50C at 1/min 50-130C at 2/min 130-180C at 4/min 180C for 20 min Split 1:300, 200C 1 L of neat sample FID, 250C Nitrogen makeup gas at 30 mL/min
Injector: Detector:
1. 2. 3. 4. 5. 6. 7. 8. 9. 10.
Methane n-Butane Isopentane n-Pentane n-Hexane Methylcyclopentane Benzene Cyclohexane Isooctane n-Heptane
*11. 12. 13. 14. 15. 16. **17. 18. 19. 20.
Toluene 2,3,3-Trimethylpentane 2-Methylheptane 4-Methylheptane n-Octane Ethylbenzene m-Xylene p-Xylene o-Xylene n-Nonane
21. 22. 23. 24. 25. 26. 27. 28. 29. 30. 31. 32. 33.
Isopropylbenzene Propylbenzene 1,2,4-Trimethylbenzene Isobutylbenzene sec-Butylbenzene n-Decane 1,2,3-Trimethylbenzene Butylbenzene n-Undecane 1,2,4,5-Tetramethylbenzene Naphthalene Dodecane Tridecane * Valley point with 12=78% **Valley point with 18=87%
C165
437

Petroleum
PONA Mix as Specified by AFNOR Method #2
Column:
P/N: Carrier: Oven: 26. 27. 28. 29. 30. 31. 32. 33. 34. 35. 36. 37. 38. 39. 40. 41. 42. 43. 44. 45. 46. 47. 48. 49. 50. 51. 2,3,3-Trimethylpentane 2-Methylheptane 3-Methylheptane n-Octane Ethylbenzene m-Xylene p-Xylene o-Xylene n-Nonane n-Propylbenzene 1,3,5-Trimethylbenzene 1,2,4-Trimethylbenzene n-Decane 1,2,3-Trimethylbenzene Indan 1,3-Diethylbenzene 1-Methyl-3-propylbenzene 1,3-Diethyl-5-ethylbenzene 1,2-Diethyl-4-ethylbenzene n-Undecane 1,2,4,5-Tetramethylbenzene 1,2,3,5-Tetramethylbenzene Naphthalene n-Dodecane 2-Methylnaphthalene Tetradecane
DB-Petro50
50 m x 0.20 mm i.d., 0.5 m 128-1056 Helium at 16.7 cm/sec, measured at 35C 10C for 15 min 10-70C at 1.3/min 70-250C at 1.7/min Split 1:200, 250C 0.3 L petroleum reformate FID, 250C Nitrogen makeup gas at 30 mL/min
Injector: Detector:
GC and GC/MS
1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. 18. 19. 20. 21. 22. 23. 24. 25.
Ethane Propane n-Butane Ethanol Isopentane 1-Pentene 2-Methyl-1-butene n-Pentane 2-Methyl-2-butene 2,2-Dimethylbutane 1-Cyclopentene Cyclopentane 2,3-Dimethylbutane 2-Methylpentane 3-Methylpentane 2-Methyl-1-pentene n-Hexane 2,2-Dimethylpentane Methylcyclopentane 2,4-Dimethylpentane Benzene 1-Methyl-1-cyclopentene Isooctane n-Heptane Toluene
C421
438

Petroleum
Aromatics in Finished GasolineASTM Method 5769
Column:
P/N: Carrier: Oven: Helium at 35 cm/sec, measured at 50C 50C for 1 min 50-190C at 2/min 190C for 1 min Split 1:100, 250C, 0.3 L unleaded gasoline MSD AccuStandard ASTM/EPA gasoline refinery aromatics calibration standard kit (M-GRA-CAL/IS-SET) 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. 18. 19. 20. 21. 22. 23. 24. 25. 26. 27. 28. 29. 30. 31. 32. 33. Methyl-tert-butyl-ether (MTBE) n-Hexane Benzene-d6 (IS) Benzene Isooctane n-Heptane Toluene n-Octane Ethylbenzene-d10 (IS) Ethylbenzene m-Xylene p-Xylene o-Xylene n-Propylbenzene 1-Methyl-3-ethylbenzene 1-Methyl-4-ethylbenzene 1,3,5-Trimethylbenzene 1-Methyl-2-ethylbenzene 1,2,4-Trimethylbenzene n-Decane 1,2,3-Trimethylbenzene Indan 1,4-Diethylbenzene n-Butylbenzene (valley) 1,2-Diethylbenzene 1,2,4,5-Tetramethylbenzene 1,2,3,5-Tetramethylbenzene Naphthalene-d8 (IS) Naphthalene n-Dodecane Pentamethylbenzene 2-Methylnaphthalene 1-Methylnaphthalene
DB-1
60 m x 0.25 mm i.d., 1.0 m 122-1063
Injector: Detector: Calibration Standard:
GC and GC/MS
C423
Simulated Distillation
Column:
DB-2887
10 m x 0.53 mm i.d., 3.0 m 125-2814 Helium at 7 mL/min 35-350C at 15/min Megabore Direct FID Nitrogen makeup gas at 30 mL/min
1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. 18. 19.
n-Pentane n-Hexane n-Heptane n-Octane n-Nonane n-Decane n-Undecane n-Dodecane n-Tetradecane n-Pentadecane n-Hexadecane n-Heptadecane n-Octadecane n-Eicosane n-Tetracosane n-Octacosane n-Dotriacontane n-Hexatriacontane n-Tetracontane
Reference Gas Oil

Column:
DB-2887
10 m x 0.53 mm i.d., 3.0 m 125-2814 Helium at 7 mL/min 35-350C at 15/min Megabore Direct FID Nitrogen makeup gas at 30 mL/min
C166
C167
439

Petroleum
Regular Unleaded Gasoline (California Phase 1)"Normal" GC Run
Column:
P/N: Carrier: Oven: 35C for 9.5 min 35-45C at 13.3/min 45C for 11 min 45-60C at 1.4/min 60C for 11 min 60-220C at 2.7/min 220C for 3.6 min Split 1:200, 0.2 L FID, 300C
DB-Petro100
100 m x 0.25 mm i.d., 0.5 m 122-10A6 Hydrogen at 31 cm/sec Injector: Detector:
Enlarged view at 55-62 min
GC and GC/MS
C513
Regular Unleaded Gasoline (California Phase 1)"Fast" GC Run

Column:
P/N: Carrier:
Oven:
DB-1
40 m x 0.10 mm i.d., 0.2 m 127-1046 Hydrogen at 34.8 cm/sec Injector: Detector:
35C for 3.6 min 35-45C at 36.1/min 45C for 4.2 min 45-60C at 3.9/min 60C for 4.2 min 60-220C at 6.9/min 220C for 1.4 min Split 1:400, 0.2 L FID, 300C
Compare Resolution
Enlarged view at 19-22 min
C514
440

Petroleum
n-Paraffin Standard
Column:
P/N: Carrier: Oven: Injector: Helium at 18 mL/min, measured at 35C -30-430C at 10/min OPTIC PTV 55-450C at 2/sec 0.5 L of about 2% n-paraffins in CS2 FID, 450C Nitrogen makeup gas at 15 mL/min
DB-HT Sim Dis

5 m x 0.53 mm i.d., 0.15 m 145-1001
Detector:
Peak numbers denote carbon number
GC and GC/MS
C526
Polyethylene
Column:
DB-1
15 m x 0.53 mm i.d., 0.15 m 125-1011 Helium at 8 mL/min 120-360C at 10/min Split 1:500, 0.5 L 3% Solution in CS2 FID, 300C Nitrogen makeup gas at 30 mL/min
C169
441
LC and LC/MS
Table of Contents
LC and LC/MS Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .443 LC General Supplies Tubing and Connectors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .444447 Solvent Filters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .448449 Degasser . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .449 Manual Injection Valves . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .450451 Injection Valve Sample Loops . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .452453 Miscellaneous Supplies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .454456 1100 Series LC Instrument Parts and Supplies Tools . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .457458 Capillaries Connectors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .459 Vacuum Degassers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .460 Pump Parts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .461 Seal Wash . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .462 Purge Valve Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .462 Seals and Pistons . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .463 Solvent Inlet Filters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .464 Pump Accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .464465 Autosampler Accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .465470 Column Compartment Accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .471 Capillary LC Accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .472473 VWD Supplies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .474476 DAD Supplies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .477481 Flourescence . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .481 RID Detector . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .482 Test Solutions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .482 LC/MA Supplies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .483484 1050 Series Instrument Parts and Supplies Pump Accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .485 Degasser . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .486 Flow Connectors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .486 Sample Introduction Supplies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .486488 VWD Supplies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .489490 MWD/DAD Supplies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .491 1090 Series Instrument Parts and Supplies General Supplies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .492 Pump and Flow Path Supplies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .492494 Sample Introduction Supplies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .495497 FPD Accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .497 DAD Supplies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .498 Detector Module Supplies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .499500 LC and LC/MS Columns . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .501599 LC and LC/MS Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .600603 LC and LC/MS Applications BioPharmaceutical . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .604617 Chemical/Industrial . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .618621 Environmental . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .622630 Food and Flavors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .631642 Pharmaceutical . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .643652 www.agilent.com/chem
LC and LC/MS
442
General LC Supplies
Introduction
Reducing your cost of ownership

LC columns and consumables from Agilent are designed for ease of use, enhanced productivity, and extended life. As a result, they help reduce the cost of ownership of your instruments. The comprehensive line of supplies shown in this section, from lamps and flow cells to tubing, fittings, and maintenance parts, are engineered by the teams that created your Agilent instrument. That means you can count on a PerfectFit for maximum reliability and detection. The range of columns offered herea broad selection of phases, particle sizes, and hardware configurationleads the industry. Chances are excellent that you will be able to match the requirements of your application. In fact, you can use our handy selection guide and application chromatograms to help you make a fast, accurate choice. Theres even a troubleshooting and maintenance guide that will send you back to this section again and again, for Agilents LC expertise as well as for our products.
LC and LC/MS
443
General LC Supplies
Tubing and Connectors
LC General Supplies
Agilent offers a wide range of supplies for operation and maintenance of LC systems. These products have been carefully designed or selected by Agilent to work with your Agilent instruments for maximum performance and uptime.
LC Tubing and Connectors

Whether you require stainless or PEEK tubing for your application, Agilent provides the tubing essential to complete your Agilent LC system.
What is the function

PEEK or stainless steel tubing or capillary tubing creates the sample flow path through your LC system, from autosampler or manual injection valve, through the column to the detector. The ideal tubing provides an inert surface, tight and leak free connections, and zero dead volumes.
Why Replace
Periodically inspect your system for Leaking or loose fittings Pinched or constricted tubing Application changes
How to Minimize Problems

Select the most narrow diameter and shortest length tubing your application and system allow to prevent peak dispersion or loss of resolution Smaller id tubing (0.12 mm) is recommended for narrowbore or microbore applications Use color code tubing or track identifiers to facilitate maintenance and troubleshooting
Stainless Steel Tubing

Precisely measured and pre-cut for a perfect fit in Agilent LCs Universal lengths Agilent 1100 capillary tubing with color coding to distinguish internal diameters
Universal Connecting Capillaries
LC and LC/MS
Made of flexible stainless steel (0.6 mm od) with 1/16 in. fittings for both ends Pre-swaged fittings are assembled according to Swagelok specifications Non-swaged capillaries include fittings, but are not assembled Note: All fittings in the 1100 HPLC are Swagelok compatible, EXCEPT the ports on Model 7725 Rheodyne valve.
Color Red Green Blue Orange
ID (mm) 0.12 0.17 0.25 0.50
444
General LC Supplies
Tubing
Universal Connective Capillaries
Length (mm) ID (mm) Connection A Pre-swaged/ non-swaged Connection B Pre-swaged/ non-swaged Recommended Agilent 1100 Use From To Part No.
130 160 320 700 90 130 180 280 380 500 600 800 900 35 70 105 150 180 280 880
0.25 0.25 0.25 0.25 0.17 0.17 0.17 0.17 0.17 0.17 0.17 0.17 0.17 0.12 0.12 0.12 0.12 0.12 0.12 0.12
p-s p-s p-s p-s n-s p-s p-s p-s p-s n-s p-s p-s p-s p-s n-s p-s p-s p-s p-s p-s
p-s p-s p-s n-s n-s n-s p-s n-s n-s n-s n-s n-s n-s p-s n-s n-s n-s p-s n-s n-s
Pump
Autosampler
01090-87308 G1313-87301
Pump Pump
Autosampler Universal (not swaged)
79835-87638 01018-67305 G1316-87300 01090-87305
Autosampler Autosampler Column
Column Column DAD
G1313-87305 01090-87304 G1315-87311 G1328-87600
Pump Autosampler Pump
Autosampler Column Manual Valve
G1312-67305 01048-87302 G1329-87300 79841-87609*
Column
Universal
G1316-87303 01090-87611
Column Autosampler Autosampler
DAD Column Column
G1315-87312
LC and LC/MS
G1313-87304 01090-87610 01040-87602*

*Made of 1.58 mm (rigid) od tubing
Note: Capillaries for the G1361A Preparative Pump can be found on page 465.
Rigid Capillary Tubing

Squarely cut, pre-cleaned and ready to use Use with SS fittings and ferrules (P/N 5062-2418) or PEEK fittings (P/N 0100-1516)
Rigid Capillary Tubing (10/pk)

Length (mm) 100 200 ID (mm) 0.17 0.17 Connection No fittings No fittings Part No. 5061-3361 5061-3362
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General LC Supplies
Tubing Flexible Stainless Steel Capillaries Without Fittings
Color coded SS tubings For use with SS fittings (P/N 5062-2418) or PEEK fingertight fittings (P/N 0100-1516)
Flexible Stainless Steel Connecting Capillaries Without Fittings

Length (mm) 105 150 280 400 105 150 280 400 ID (mm) 0.17 0.17 0.17 0.17 0.12 0.12 0.12 0.12 Color Green Green Green Green Red Red Red Red Part No. 5021-1816 5021-1817 5021-1818 5021-1819 5021-1820 5021-1821 5021-1822 5021-1823
Capillary Kits With Flexible Stainless Steel Capillaries and 1/16 in. Male Fittings
Description Capillary kit containing 0.12 mm id capillaries: 3 each 105 mm long, 1 each 150 mm long, 1 each 280 mm long, plus fittings Capillary kit contains preswaged 0.12 mm id capillaries: 3 each 70 mm long, 1 each 280 mm long, 1 each 35 mm long Part No. 5061-3304
5061-3315
LC and LC/MS
PEEK Tubing
Flexible and easy to cut to desired lengths Color coded for easy tracking Accepts both stainless and PEEK fittings 1/16 in. od
PEEK Tubing
Length (m) 1.5 1.5 5 1.5 5 1.5 5 ID (mm) 0.50 0.25 0.25 0.18 0.18 0.13 0.13 Color Orange Blue Blue Yellow Yellow Red Red Part No. 0890-1761 0890-1762 5042-6463 0890-1763 5042-6462 0890-1915 5042-6461
446
General LC Supplies
Tubing/Fittings Tubing Cutter
Description Cutter for plastic tubing Replacement blades for tubing cutter 5/pk Unit Part No. 8710-1930 8710-1931
Fittings
To ensure leak free connections, use the manufactured recommended style fittings with columns, valves, and unions. Peak shape and resolution may be lost by mixing fittings. Different column fitting requirements (ZORBAX columns use standard Swagelock fittings) Agilent 1100 modules use standard Swagelock fittings Rheodyne injection valves require Rheodyne fittings or long SS fittings
Tubing Cutter P/N 8710-1930 Replacement Blades P/N 8710-1931
Fittings
Description PEEK RheFlex 2-piece fittings PEEK RheFlex 2-piece fittings, colored ChromTrac identifiers, 2 black, 2 white 2 green, 2 gray, 4 yellow, 4 red, 4 blue 1/16 in. SS fittings, front and back ferrules 1/16 in. SS long fittings, front and back ferrules 1/16 in. SS male fittings 1/16 in. SS front ferrules 1/16 in. SS back ferrules Zero dead-volume fitting Finger-tight PEEK fittings, 1/16 in. Finger-tight PEEK long fittings, 1/16 in. Finger-tight PEEK fittings, 1/16 in., yellow, blue, black, green, red, 2 each 10/pk 10/pk 10/pk Unit 5/pk 10/pk 20/pk 10/pk 10/pk 10/pk 10/pk 10/pk Part No. 0100-1631 0100-2175 0350-1402 5062-2418
ChromTrac Identifier P/N 0350-1402
LC and LC/MS
5065-4454 5061-3303 5180-4108 5180-4114 0100-0900 5063-6591 5062-8541 5065-4426

Fittings P/N 5062-2418
PEEK Finger-Tight Fittings
447
General LC Supplies
Solvent Filters
Filter Systems
The use of the appropriate filters in the LC system will prevent blockage. Agilent offers two types of filter kits for use with any HPLC system.
What is the Function

Prevent blockage of the column inlet frit from contamination Maintain column efficiency and back pressure
Why Replace
Protect and extend column lifetime Increase system uptime

Use high capacity filters Ensure even distribution of solvent over filter surface
In Line Filters
Low Volume Column Inlet Filter
Positioned immediately before the LC column Removes particles from the sample and the injection system Minimizes external band spreading due to frit diameter of only 2.1 mm and tapered inserts Can be used with any microbore, highspeed, or standard analytical columns
In Line Filter
Description Low volume column inlet filter kit: includes filter holder, 2 frits, 60 x 0.12 mm tubing. Install between injector and column Replacement frits, 2 m, 2.1 mm
Unit
Part No. 01090-68702
10/pk
280959-904
Universal Solvent Filter

Installed between the LC pump and the injector to remove particles from the solvent Uses a high capacity filter Filter assembly consists of 4.8 mm frit, two inserts and a two-piece holder Frit is placed between the tapered edges of the inserts so the solvent is evenly distributed over the filtering frit
Description Universal solvent filter kit: includes filter holder, 2 frits, 130 x 0.25 mm tubing. Install between pump and injector Replacement frits, 2 m, 4.8 mm for solvent filter 2/pk Unit Part No. 01090-68703 01090-27609
LC and LC/MS
448
General LC Supplies
Solvent Filters/Degasser
Solvent Filters/Degassers
What is the function
Mobil phase filters can improve the performance of your LC system by Removing contaminants from solvents Degassing solvents in systems lacking online degassers
Why use
Filtered solvents decrease piston wear from particulates Increase column life Eliminate pump downtime caused by air locks in check valves

Filter into safe plastic-coated or heavywalled solvent reservoirs
Description Filter degasser assembly with 40 in. long, 1/4 in. od Teflon tubing, 10 filter membranes, and a 1 liter plastic coated graduated solvent reservoir Filter degasser assembly without solvent reservoir with 40 in. od Teflon tubing, 10 filter membranes Solvent filtration kit: 250 mL glass reservoir, tapered funnel base with PTEE coated seive, tapered top, 1000 mL flask and clamp Cellulose filter membranes 47 mm, pore size 0.45 m Nylon filter membranes 47 mm, pore size 0.45 m Teflon filter membranes 47 mm, pore size 2 m
Unit
Part No. 3150-0507
3150-0508 3150-0577 100/pk 100/pk 10/pk 3150-0576 9301-0895 3150-0509

Solvent Filter Degasser P/N 3150-0507
LC and LC/MS
Glass Solvent Filter Degasser P/N 3150-0577
449
General LC Supplies
Manual Injection Valves

Agilent provides the latest developments in LC injection valve technology from Rheodyne. Continuous flow path with make-before-break design Sample capacity Choice of stainless or PEEK flow path Easy access to fittings due to wide 30 port angles
Rheodyne Series 7725i

Stainless steel Most popular injection valve for analytical HPLC Loops range from 1 L to 5 mL (20 L loop installed) Built in position sensing switch provides the LC with reproducible start signals Selected as Agilent 1050 and 1100 manual injectors
Description Manual injection valve, 7725i, SS Part No. 5063-6502

PEEK flow path is perfect for biomoleculessample does not contact metal Similar features as 7725i, including the make-before-break design
Description Manual injection valve, 9725i, PEEK Part No. 0101-1253
LC and LC/MS
Large sample volumes, high flow rates and preparative columns 100 L to 20 mL (10 mL loop installed) For biomolecules, the 3725i has PEEK materials
Description Manual injection valve, Rheodyne 3725i, SS, prep valve Manual injection valve, Rheodyne 3725i, PEEK, prep valve Part No. 0101-1232 0101-1231
Helpful Hint: Autosampler and column switching valves and maintenance parts for the Agilent 1100 system can be found on pages 466471.
450
General LC Supplies
Maintenance and Replacement Parts for Manual Injection Valves
Rotor seals wear with use and need routine replacements Stators only need replacement if the ports are damaged PEEK rotor seals are incompatible with concentrated nitric and sulfuric acids
Valve Replacement parts

Description
Rotor Seals
Part No. 0101-0623 0101-0620 0101-1255 0101-1233 0101-0624 0100-1859

Stators and Seals
Vespel: operating pH 0 to 10 (7125, 7725) Tefzel: operating pH 0 to 14 (7125, 7725) PEEK: pH 0 to 14 (7125, 7725, 9725) PEEK: operating pH 0 to 14 (3725)
Stator Face Assembly
Rheodyne Series 7125 Rheodyne Series 7725

Stators
Rheodyne Series 7725
0100-1860
LC and LC/MS
451
General LC Supplies
Sample Loops
Injection Valve Sample Loops

The right mix of injection valve sample loops are available for your application needs. Agilent offers factory-cut and finished loops of the highest quality. SS loops are square cut, free of burrs, for a flush connection Flexible PEEK loops have a clean, straight cut for low dead volume connections
Stainless Steel Sample Loops

Sample loops for Rheodyne 7725 Series and 7125 Series valves are not interchangeable due to the change in port angle Actual volumes can differ due to tolerance of metal tubing bore Accuracy of large metal loops is 5%, intermediate loops 10%, small loops 30%
Stainless Steel Loops for 7125 and 7010 Injectors

Description 5 L sample loop 10 L sample loop 20 L sample loop 50 L sample loop 100 L sample loop 200 L sample loop 500 L sample loop
Stainless Steel Sample Loop
ID (mm) 0.18 0.30 0.51 0.51 0.51 0.76 0.76 0.76 1.00 1.00
Part No. 1535-4860 0101-0376 0101-0377 0101-0378 0101-0379 0101-1252 0101-1251 0101-1219 0101-1250 0101-1249
1 mL sample loop 2 mL sample loop 5 mL sample loop
LC and LC/MS
Stainless Steel Loops for 7725 Injectors

Description 5 L sample loop 10 L sample loop 20 L sample loop 50 L sample loop 100 L sample loop 200 L sample loop 500 L sample loop 1 mL sample loop 2 mL sample loop 5 mL sample loop ID (mm) 0.18 0.30 0.30 0.51 0.51 0.76 0.76 0.76 1.00 1.00 Part No. 0101-1248 0100-1923 0100-1922 0100-1924 0100-1921 0101-1247 0101-1246 0101-1245 0101-1244 0101-1243
452
General LC Supplies
Sample Loops PEEK Sample Loops
Inert to most organic solvents Wall thickness, temperature, exposure time and concentration of organic solvents affect the durability of PEEK tubing Concentrated nitric acid and sulfuric acid weaken PEEK tubing THF, methylene chloride and DMSO cause PEEK to swell Actual volumes can differ because of tolerance of tubing bore Accuracy of large PEEK loops is 14%, intermediate loops 21%, small loops 65%
PEEK Sample Loops for 9725 Injectors

Description 5 L sample loop 10 L sample loop 20 L sample loop 50 L sample loop 100 L sample loop 200 L sample loop 500 L sample loop 1 mL sample loop 2 mL sample loop 5 mL sample loop ID (mm) 0.18 0.25 0.25 0.51 0.51 0.51 0.76 0.76 0.76 0.76 Part No. 0101-1241 0101-1240 0101-1239 0101-1238 0101-1242 0101-1237 0101-1236 0101-1235 0101-1234 0101-1230
Peek Sample Loop
PEEK Sample Loops for 3725 Injectors

Description 2.0 mL sample loop 5.0 mL sample loop 10.0 mL sample loop 20.0 mL sample loop ID (mm) 1.6 1.6 2.0 2.0 Part No. 0101-1229 0101-1228 0101-1227 0101-1226
LC and LC/MS
Titanium Sample Loops

Titanium Sample Loops for 7725 Injectors
Description 20 L sample loop 100 L sample loop 200 L sample loop 500 L sample loop 2 mL sample loop Part No. 0101-0655 0101-0656 0101-0657 0101-0658 0101-0670
453
General LC Supplies
Manual Injection Valve Syringes Syringes for Manual Injection Valves
The syringes for manual injection have a blunt-tip point style needle to prevent damaging the valves internal parts. They can be used with any type/brand of manual injection valve.
Syringes for Manual Injection Valves

Volume 10 L 10 L 25 L 25 L 50 L 50 L 100 L 100 L 250 L 250 L 500 L 500 L Needle Type Removeable needle Fixed needle Removeable needle Fixed needle Removeable needle Fixed needle Removeable needle Fixed needle Removeable needle Fixed needle Removeable needle Fixed needle Part No. 5182-9725 5182-9644 5182-9719 5182-9628 5183-4538 5182-9619 5183-4539 5182-9613 5182-9720 5182-9624 5183-4540 5182-9658
Agilent LC Supplies at a Glance

LC and LC/MS
Our policy of continuous quality improvement extends from the most complex system to the smallest consumable item. Continued development of our lamp offering to provide the highest performance and longest life Maximum durability of high purity, mono-crystalline sapphire pistons Overall precision in design and alignment to provide the PerfectFit to get the most from your investment For your convenience, popular supplies for Agilent model LCs are listed in the tables that follow. More detail on product selection, as well as the full complement of supplies and consumables to maintain your Agilent LC, follows in the section dedicated to Agilent 1100, 1050, 1090 series LC instruments.
454

HPLC Detector Lamps
Model 1100 Series Configuration G1314A VWD G1315A DAD, G1365A MWD G1315 DAD, G1365A MWD Long life 1050 Series 79853A VWD 79853C VWD, 79854A MWD, G1306A DAD 1090 Series G1321A FLD 79881A FPD 79880A DAD Lamp Type Deuterium Deuterium Deuterium Tungsten Deuterium Deuterium Part No. G1314-60100 2140-0590 5181-1530 G1103-60001 79853-60002 79883-60002
Deuterium Lamp P/N G1314-60100
Deuterium Deuterium Xenon Flashlamp
79880-60002 79883-60002 2140-0600

Deuterium Lamp for VWD, MWD, and DAD P/N 79883-60002
Sapphire Pistons for LC Pumps and Autosamplers

Description Sapphire piston for 1100 and 1050 Sapphire piston, 2 mm for G1329A -LC autosampler Sapphire piston for G1361 prep pump Sapphire piston for HP 1090 pump Sapphire piston for 900 L sampler option Part No. 5063-6586 5064-8293 G1361-22402 3980-0672 5062-8587
Piston Seals
Description Piston seal for 1100 and 1050 Piston seal, polyethylene for normal phase applications for 1100 and 1050 Piston seal, 2 mm for G1329A -LC autosampler Piston seals for HP 1090 Piston seal for buffered solutions for HP 1090 4/pk Unit 2/pk 2/pk Part No. 5063-6589 0905-1420 5022-2175 5062-2494 0905-1194
Pre-aligned lamps for Agilent are easy to replace
LC and LC/MS
Sapphire piston and seals
455

Detector Flow Cells
Cell Standard Micro High pressure Semi-micro flow cell Standard Semi-micro Micro High Pressure Micro/high pressure 500 nl flow cell Preparative SS Preparative Quartz Preparative Quartz Fluorescence Detector Optional cuvette Standard Max Press Volume (L) Path Length (mm) Part No. 40 bar 40 bar 400 bar 40 bar 120 bar 120 bar 400 bar 400 bar 50 bar 120 bar 20 bar 20 bar 20 bar 14 1 14 5 13 4.5 1.7 1.7 500 nl 4 na na 8 10 5 10 6 10 6 6 6 10 3.0 0.3 0.06 G1314-60086 G1314-60081 G1314-60082 G1314-60083 G1315-60012 G1315-60011 79883-60055 G1315-60015 G1315-68714 G1315-60016 G1315-60017 G1315-60018 G1321-60005 G1321-60007 79881-60002
1100 Series Variable Wavelength Detector, (VWD) G1314A
1100 Series Diode Array Detector, G1315A DAD
1100 Series Fluorescence Detector, G1321A Standard
1090 Filter Photometric Detector (FPD)
100 bar
4.5
1090 Diode Array Detector (DAD) Series II
Standard
120 bar 120 bar 400 bar
8 13 1.7
6 10 6
79883-60091 79883-60092 79883-60055
LC and LC/MS
High sensitivity Micro High Pressure
1050 Series Variable Wavelength Detector, (VWD) 79853C
Standard (D version) High pressure Micro Titanium Preparative, Titanium
40 bar 400 bar 40 bar 40 bar 40 bar
14 14 1 14 1.3-13
8 8 5 8 1-10
79853-60000 79853-60013 79853-60010 79853-60011 79853-60012
1050 Series Multiple Wavelength Detector, 79854A Series II Diode Array Detector, G1306A
Standard High sensitivity

Flow Cell 79853C
120 bar 120 bar 400 bar 400 bar
8 13 13 1.7
6 10 10 6
79883-60051 79883-60052 79883-60054 79883-60055
High pressure Micro high pressure
Helpful Hint: For selection of flow cell for Agilent 1100 please refer to pages 474, 477.
456
1100 Series Instrument Parts and Supplies Agilent 1100 Series LC Parts and Supplies
The Agilent 1100 Series HPLC modules were designed for long hours of operation and ease of use. To keep your system operating at optimal performance, scheduled routine maintenance is advised. Regular attention to these tasks will further increase your lab's productivity.
Recommended routine maintenance procedures for the Agilent 1100 Series LC Modules
Clean or replace the solvent inlet filter Flush the degasser Perform pump maintenance Perform autosampler maintenance Perform column switching valve maintenance (if installed) Perform detector maintenance The time needed is approximately 4 hours for a complete system. The frequency of maintenance is dependent upon your usage and applications.
Failure to maintain your HPLC can have these results

Variations in flow will vary area counts, causing poor precision of quantitative analyses Noisy baselines make integration and peak recognition difficult Poor injection volume precision may result if the autosampler is not maintained Retention time fluctuations can lead to improper peak identification If you regularly use UV spectra, the quality of the spectra will be compromised, jeopardizing peak id. Agilent offers carefully planned, effective training courses on operating, maintaining or troubleshooting your 1100. We also offer real-time, interactive e-Seminars. For details, visit our website www.agilent.com/chem
LC and LC/MS
457
1100 Series Instrument Parts and Supplies

Tools/Capillaries
Your Agilent LC system arrives with a full complement of tools needed to perform general maintenance and operation procedures. Should you need additional or replacement tools, Agilent offers a selection of high-precision, high-quality (stainless steel) tools, to avoid any deformation of the screws or nuts. Please refer to pages 8991 for a listing of general laboratory tools. Agilent offers the following specialized LC tools for your convenience:
1100 Tool Kits

Description Tool kit hex keys, Rheotool Specs Includes 3 hex keys, 4 mm, 1.5 mm, 9/64 in., with straight or T-handle, plus 1 Rheotool. The tools are recommended for easier repair and maintenance of LC modules. Used with Nanoliter flow cell for Diode Array Detector to mount capillaries of P/N G1315-68714 cell Part No. 5064-8211
Torque adapter
G1315-45003
Insert tool (seal wash option) Mounting tool for flangeless nuts
01018-23702 0100-1710
Capillary Connectors for Standard 1100 Series Modules

LC and LC/MS
For convenience, Agilent color codes capillary tubing by id.
Color Red Green Blue Orange ID (mm) 0.12 0.17 0.25 0.50
458

Capillaries
1100 Module Flexible Capillary Connectors
From: To: Length (cm) ID (mm) Material Color Fittings (1/16 in. male) Part No.
Pump
Autosampler
60 13 32
0.17 0.25 0.25 0.17 0.17 0.25 0.25 0.1 0.12 0.12 0.17 0.17 0.17 0.17 0.17 0.12 0.17 0.12 0.17 0.17 0.12 0.12 0.12 0.12 0.12 0.17 0.17 0.17 0.17 0.17 0.25+ 0.25
SS SS SS SS SS SS Ta SS SS SS SS SS SS SS SS SS SS SS SS PEEK SS SS SS SS SS SS SS SS SS SS PTFE PEEK
Green Blue Blue Green Green Blue Blue Red Red Red Green Green Green Green Green Red Green Red Green Red Red Red Red Red Green Green Green Green Green
Pre-swaged Pre-swaged Pre-swaged Not swaged Pre-swaged 1 end pre-swaged 1 end pre-swaged 1 end pre-swaged 1 end pre-swaged 1 end pre-swaged 1 end pre-swaged 1 end pre-swaged 1 end pre-swaged 1 end pre-swaged Not swaged 1 end pre-swaged 1 end pre-swaged Pre-swaged Pre-swaged Not swaged Without fittings Without fittings Without fittings Without fittings Not swaged Without fittings Without fittings Without fittings Without fittings Not swaged With fittings With fittings
G1312-67305 01090-87308 79835-87638 G1328-87600 G1329-87300 01018-67305 01019-67305 G1313-87304 01090-87610 01090-87611 G1313-87305 01090-87304 01048-87302 01090-87305 G1328-87600 G1316-87303 G1316-87300 G1315-87312 G1315-87311 5062-8522
Thermostated Autosampler Manual Valve Universal Autosampler Column
50 90 70 70 18 28 10,5 18 28 80 13
Manual valve
Column
50 7 9 15 38 40 10.5 15 28 40 7 10.5 15 28 40 9
Column compartment Column Column DAD VWD Universal
LC and LC
5021-1820 5021-1821 5021-1822 5021-1823 G1316-87303 5021-1816 5021-1817 5021-1818 5021-1819 G1316-87300 0890-1713 5062-8535
DAD VWD
Outlet Outlet
50 48
459

Vacuum Degasser
1100 Series Vacuum Degassers

A vacuum degasser is recommended for
Maximum sensitivity in the low UV wavelength range High injection precision High retention time reproducibility Flow rates below 0.5 mL/min
Maintenance of the vacuum degasser

To generally clean the vacuum degasser tubing, flush the system with isopropanol Flush the degasser with water after using buffers Speed solvent changes by drawing solvent through the degasser and tubing with the syringe adapter kit
G1322A 1100 Series Vacuum Degasser

Description Online degasser accessory kit: includes 8 screws, 8 bushings, 4 markers, tubing, syringe, and syringe adapter Tubing kit (from degasser to pump): includes 30 cm pieces of tubing with premounted screws and bushings Glass filter, solvent inlet Disposable syringes, luer, 20 mL Syringe adapter, 1/16 in. od, 2 in. long
Tubing Kit P/N G1322-67300
Unit
Part No. G1322-68705
4/pk
G1322-67300 5041-2168
10/pk
5062-8534 9301-1337
G1379A 1100 Series Micro Vacuum Degasser

Description Dual-channel micro degasser vacuum chamber Vacuum tube set Mounting tool for degasser screws Part No. G1379-60001 G1379-67300 0100-1710
LC and LC/MS
460

Pump Supplies
1100 Series Pump Maintenance Parts

The Agilent 1100 Series family of HPLC modules has several different pump units to choose from: isocratic, quaternary, binary, capillary, and preparative. The 1100 was designed for ease of useall pump maintenance parts can be accessed from the front by simply removing the front cover. Scheduled routine maintenance of these pumps based on your application is recommended to achieve maximum uptime.
1100 Pump routine maintenance procedures:

Replacing the seals and pistons Replacing the PTFE frit Replacing the cartridge on the Active Inlet Valve Cleaning the outlet ball valve Cleaning or replacing the solvent inlet frits Routine pump maintenance should be done on a regular basis to keep your Agilent 1100 system performing at its optimum. You may perform all the maintenance at once or perform maintenance on an as-needed basis. Some parts may need to be replaced more than others depending upon your application and solvent preparation procedures. Helpful Hint: For a complete selection of vials and syringes, see the General Chromatography section of this catalog.
LC and LC/MS
461

Seal Wash/Purge Valve Seal Wash
The seal wash option can be installed to protect your pistons and seals from the routine use of highly concentrated buffer solutions (>0.01M).
Description Seal wash option kit: includes 2 film washers, 4 m flex tubing, 2 pump seals, 1 flow regulator, 2 support rings, 2 support ring assemblies, 5 mL luer lock syringe, tool, knife, and abrasive paper Support seal assembly Seal keeper Wash seal Insert tool for wash seal Wash seal gasket 6/pk Unit Part No. 01018-68722
5001-3739 5001-3743 0905-1175 01018-23702 5062-2484
Note: Not applicable for the G1361A preparative pump, preparative pump parts on page 465.
Purge Valve Maintenance

Common to all 1100 pump modules, the purge valve has a gold seal held in place with a plastic cup. The most frequently replaced item in the 1100 Series pumps is the purge valve PTFE frit (P/N 01018-22707), which can clog with seal material or solvent particulates. When replacing the frit, remember that the cutout in the frit goes on top so that more surface area will be provided on the side facing the solvent flow.
LC and LC/MS
The gold seal should be replaced after the valve has been removed a few times.
Description PTFE frit Outlet gold seal Outlet cap Replacement purge valve 4/pk Unit 5/pk Part No. 01018-22707 5001-3707 5062-2485 G1311-60009
462

Purge Valve/AIV/Piston Cleaning the Outlet Ball Valve
Before exchanging the outlet ball valve, try cleaning it in a sonicator by placing the valve in an upright position (onto the plastic cap) in a small beaker with alcohol. Sonicate for 5 to 10 minutes. The 1100 Series binary pump has a different outlet ball valve which includes a sieve.
Description Outlet ball valve (quaternary, isocratic) Outlet ball valve (binary) Gold seal Cap Binary pump outlet valve SS sieve 10/pk Unit Part No. G1311-60012 G1312-60012 5001-3707 5062-2485 5063-6505
Replacing the Active Inlet Valve (AIV)

The 1100 Active Inlet Valve was redesigned to include an exchangeable cartridge for easier maintenance, replacing the Gold Seal end cap. Cartridges are not compatible with old style AIV.
Description Cartridge Active inlet valve Part No. 5062-8562 G1312-60010
Replacing Seals and Pistons

Pump seals should be replaced when: There are leaks on the bottom of the pump head Retention times are unstable The pressure ripple is unstable Each time the seals are replaced, the pistons (plungers) should be examined for scratches. Scratched pistons will cause small leaks and can damage the seals.
Description Sapphire Piston Piston Seals, graphite filled Teflon (reversed phase) Piston Seals, polyethylene (normal phase) Lubricant 2/pk 2/pk Unit Part No. 5063-6586 5063-6589 0905-1420 79846-65501
LC and LC/MS
463

Inlet Filter Supplies/Pump Accessories Solvent Inlet Filters for G1311/12/54A Pumps
Unfiltered solvents or microbial growth in the solvent bottle will reduce the lifetime of the solvent inlet filter and will influence the pump performance. Remember to clean or replace the solvent inlet filters on a regular basis, perhaps every 3 months.
Description Frit adapter, 3 mm PTFE (use with P/N 5041-2168) Glass filter, solvent inlet SS Filter, Solvent inlet Unit 4/pk Part No. 5062-8517 5041-2168 01018-60025
Pump Accessories
G1311/12/54A Standard Pump
Description Bottle head assembly with filter for F29/32 tapered inlet bottle Bottle head assembly, screw bottle, with drawing tube and filter Solvent reservoir, screw bottle, 1 L Solvent reservoir, screw bottle, 1 L, with cap Solvent reservoir, 1 L screw top bottle, amber
Bottle Cap with 3-hole insert P/N 5063-6531
Part No. G1312-68706 G1311-60003 9301-1420 9301-1421 9301-1450 5063-6531 G1312-87330 G1312-67301 G1322-67300 4/pk
Bottle cap with 3-hole insert Solvent mixer Capillary, damper to purge valve (to bypass binary pump mixer) Tubing kit, from degasser to pump: includes 30 cm pieces of tubing with premounted screws and bushings
LC and LC/MS
G1382A 1100 Series Capillary Pump

Description Accessory kit for capillary pump Filter assembly with 0.5 m pore size filter Replacement frits, 0.5 m for P/N 5064-8273 Bottle head assembly with tubing and filter 10/pk Unit Part No. G1376-68705 5064-8273 5022-2185 G1376-60003
464

Pump Accessories/Fraction Collector
G1361A 1100 Series Preparative Pump
Description Valve assembly, inlet/outlet Sapphire plunger O-ring, Viton Peristaltic pump for use with 1100 Series LC Frit adapter (use with P/N 3150-0944) Glass filter, solvent inlet Bottle, clear, 2 L, 2 inlets Bottle, amber, 2 L Bottle, clear, 2 L Bottle head assembly SS filter assembly with PEEK ring 2 m pore size SS capillary outlet valve 1 to multi assembly, male/male 0.6 x 173 mm SS capillary outlet valve 1 to multi assembly, male/male 0.5 x 178 mm SS capillary EMPV to next module, male/male SS capillary EMPV to multi assembly, male/male SS capillary union to EMPV2, male/female SS capillary union to mixer, male/female SS capillary EMPV1 to union, male/female 0.6 x 400 mm 0.5 x 160 mm 0.6 x 111 mm 0.6 x 40 mm 0.6 x 367 mm 30 mm Specs Part No. G1361-60012 G1361-22402 0905-1516 5065-4445 G1361-23204 40 m pore size 3150-0944 5065-4421 9301-6341 9301-6342 G1361-60002 5022-2192 G1361-67300 G1361-67301 G1361-67302 G1361-67303 G1361-67304 G1361-67305 G1361-67306
Prep Bottles
Fraction Collector
G1364A 1100 Series Fraction Collector
Description Tray, 4 well plates, cooled Tray, 2 well plates, 10 funnels, cooled Tray holding 40 tubes Tray holding 60 tubes Tray holding 126 tubes Tray holding 215 tubes Round bottom glass vial Round bottom glass vial Round bottom glass vial Funnel seals Preparative tubing kit Analytical tubing kit 0.8 mm 0.25 mm 30 x 100 mm, 50 mL 25 x 100 mm, 35 mL 16 x 100 mm, 14 mL 12 x 100 mm, 7 mL 30 x 100 mm 25 x 100 mm 30 x 48 mm 100/pk 100/pk 100/pk 10/pk Specs Unit Part No. G1364-84501 G1364-84502 G1364-84503 G1364-84504 G1364-84505 G1364-84506 5042-6458 5042-6459 5042-6470 G1364-68730 G1364-68711 G1364-68712
LC and LC/MS
465

Standard Autosampler Accessories
1100 Standard Autosampler Maintenance Parts and Supplies

G1313/27A Autosampler Kits
Description Maintenance Kit: Includes 1 rotor seal (Vespel), 1 needle, 1 needle seat, 2 metering seals, 15 fingercaps Autosampler Accessory Kit: Includes 2 hex keys, 1 wrench, tubing, 1 label halftray, wrist strap, 15 fingercaps, and capillary Part No. G1313-68709
G1313-68705
Injection Valve Maintenance

The most common injection valve maintenance procedure is replacement of the rotor seal. To remove the stator face and disassemble the valve, you need a 9/64 in. Allen wrench. Screws require metric hexagonal keys. Manual injection valves and accessories for the Agilent 1100 Series can be found on pages 450451.
Description Rotor, Vespel, pH 2.3 to 9.5 Rotor, Tefzel, extreme pH ranges >10 Stator head Stator screws Part No. 0100-1853 0100-1849 0100-1850 1535-4857 0100-1851 0100-1852 0101-0921
LC and LC/MS
Injection Valve Assembly P/N 0101-0921
Stator face Isolation seal Injection valve assembly (Rheodyne 7750)
Note: Not applicable to G1377/78/87A micro autosamplers, please see pages 469470.
466

Standard Autosampler Accessories Needle and Needle Seat Maintenance
Replace the needle when it is bent, burred, or blunt The needle seat should be replaced when it is leaking or plugged. Leaking may result in buffer crystals on the needle seat.
Needle and Needle Seat Replacement

Description Needle Needle, 900 L Needle seat, 0.17 mm id capillary Needle seat, 0.12 mm id capillary Needle seat, 0.17 mm id capillary, PEEK Needle Arm Kit includes needle clamp, 2 clamp screws
Vial Gripper Maintenance
Unit
Part No. G1313-87201 G1313-87202 G1313-87101 G1313-87103 G1313-87102 G1313-68713
Finger caps
15/pk
5063-6506
Metering Device
Infrequently, the metering device seal and piston may need replacement if you see loss in injection volume precision or metering device leaking.
Metering Device Maintenance

Description Sapphire piston Piston seals Metering valve seal Sapphire piston assembly Capillary to metering device
Multidraw Upgrade Kit
Specs
Unit 2/pk
Part No. 5063-6586 5063-6589 0905-1294 5062-8587 G1313-87301
900 L 900 L
LC and LC/MS
Includes: 0.5 mL capillary, 1.5 mL capillary and ZDV union Seat capillary Seat capillary Union, SS zero dead volume 500 L, 0.5 mm id 1500 L, 0.9 mm id 1/16 in.
G1313-68711 G1313-87307 G1313-87308 0100-0900
467

Standard Autosampler Accessories
Standard Autosampler Accessories (G1313/27A Autosamplers)
Description 100 position tray 100 position tray, thermostattable 40 position tray 15 position tray External tray, 17 position (disposal position) Disposal tube Waste tubing, 5 m, 6.5 mm id, corrugated polypropylene for external vial tray for 2 mL vials for 6 mL vials Usage for 2 mL vials Part No. G1313-44500 G1329-60001 G1313-44502 G1313-44503 G1313-60004 G1313-27302 5062-2463
6 mL Vials and Caps for 1100 Series Autosampler

Description (100/pk) Clear glass screw cap, 16 mm cap size Clear glass crimp cap, 20 mm cap size Screw caps, 16 mm PTFE/silicone septa, 16 mm PTFE/silicone crimp caps, 20 mm Part No. 9301-1377 9301-1419 9301-1379 9301-1378 9301-1425
LC and LC/MS
468

Micro/Well Plate Autosampler Accessories
G1387A 1100 Series Micro Autosampler
Description Accessory kit sampler Injection valve Rotor seal, Vespel, 2 grooves Stator 6 fittings, 2 plugs, PEEK Sapphire plunger assembly, 2 mm Plunger seal, 2 mm Seal support assembly Seat assembly, 100 m Seat assembly, 50 m Needle Part No. G1329-68715 0101-1050 0100-2088 0100-2089 5065-4410 5064-8293 5022-2175 G1377-60002 G1329-87101 G1329-87103 G1329-80001
G1367A 1100 Series Well Plate Autosampler

Description Accessory kit for well plate sampler Needle assembly Seal assembly MTP Loop capillary assembly Peristaltic pump Tray for well plate sampler Part No. G1367-68705 G1367-87200 G1367-87101 G1367-87300 5065-4445 G1367-60001
Well Plate Autosampler
LC and LC/MS
Well Plates for Well Plate Autosamplers

Description 96 well plates, PP 96 well plates, PP 96 deep well plates, PP 384 well plates, PP 96 well plates with glass inserts, caps and septa pre-assembled Closing mats for 96 well plate 0.35 mL 50/pk Specs 0.5 mL 0.5 mL 1 mL Unit 120/pk 10/pk 50/pk 30/pk Part No. 5042-1385 5042-1386 5042-6454 5042-1388 5065-4402 5042-1389
Micro Well Plates
469

Micro/Well Plate Autosampler Accessories
G1377A 1100 Series Micro Well Plate Autosampler
Description Seat assembly for G1377 -well plate sampler Seat capillary, 150 mm, 50 m Seat capillary, 150 mm, 100 m Needle assembly for well plate sampler Loop capillary, 8 L Loop capillary, 40 L SS capillary, 0.25 mm id, 12 cm long, connecting Rheodyne valve - waste Part No. G1377-87101 G1375-87300 G1375-87317 G1377-87200 G1375-87315 G1377-87300 G1377-87301
G2250A 220 Micro Plate Sampler

Description 205H rack, two 96 deep well plates 200 rack, 13 x 100 mm tubes (9 mL), 96 207 rack, 16 x 100 mm tubes (12 mL), 75 209 rack, 12 x 32 mm tubes (2 mL), 96 94A Special holding 1100 tray 100 L syringe 1000 L syringe 5 L loop Part No. G2250-04504 G2250-04503 G2250-04502 G2250-04501 G2250-04500 G2250-24501 G2250-24500 1535-4860 0101-0377 0101-0378 0101-0379 0101-0282 G2250-23200 G2250-47100 G2250-87300
LC and LC/MS
20 L loop 50 L loop 100 L loop 500 L loop Beveled tip needle Injection port seal Capillary, 1 m, 0.17 mm id, 1/16 in. male
Note: For more loops see pages 452453.
470

Column Compartment Accessories
Column Compartment Accessories

Column Switching Valve (Rheodyne 7750) Replacement Parts
Description Rotor seal, Vespel Rotor seal, Tefzel Stator face, ceramic Isolation seal Column switching valve Part No. 0100-1855 0100-1854 0100-1851 0100-1852 0101-0920
G1316A 1100 Series Thermostated Column Compartment

Description Column identification module Column clamp Temperature calibration kit: Includes spring clamp, 30 thermal pads
Capillaries
Unit 3/pk 6/pk
Part No. 5062-8588 5063-6526 G1316-68707
Column connecting capillary, 7 cm, 0.12 mm id, 1/16 in. male/male connector Column connecting capillary, 19 cm, 0.17 mm id, 1/16 in. male/male connector Column connecting capillary, 18 cm, 0.12 mm id, 1/16 in. male/male connector Column connecting capillary, 18 cm, 0.17 mm id, 1/16 in. male/male connector
G1316-87303 G1316-87300 G1313-87304
LC and LC/MS
G1313-87305
Column Accessories for Capillary LC

Description Column switching valve kit with 4 capillaries and fittings Rotor seal, Vespel, 3 grooves-column switching Stator Column holder for -LC columns Part No. 5065-4447 0100-2087 0100-2089 5001-3702
471

Capillary LC Accessories Connecting Capillaries for the 1100 Series Capillary LC
Generic Connecting Capillaries for Capillary-LC System
Item 1 2
Generic Connecting Capillaries
From SSV Piston 1 Pump Mixing capillary Damper Mixer Filter Needle seat
To AIV Piston 2 Restriction capillary Damper Mixer Filter EMPV Injection valve
Fitting A/A A/A A/A A/A A/A A/A -/C
Part No. G1311-67304 G1312-67300 G1312-67302 G1312-67304 01090-87308 01090-87308 G1375-87400 G1329-87101
3 4 5 5 6 7
PEEK Coated Fused Silica Capillaries for Use with 20 L/min Flow Range
Item a b c d e f g h From EMPV Flowsensor Injection valve Metering device Injection valve Injection valve Column Detector -switching valve To Flowsensor Injection valve Metering device Needle Column Waste Detector Waste Column Fitting ID (m) Part No. B/B B/C B/C B/B C/D C/D/E E/C/D 50 50 50 100 50 100 50 75 50 G1375-87301 G1375-87310 G1375-87302 G1375-87303 G1375-87304 G1375-87307 G1315-68703 G1315-68708 G1375-87309
LC and LC/MS
SSV
EMPV
Pumphead A 1 2 1
Pumphead B 5 2 Mixer
Filter 6
3 5 b 4
Flow sensor
Damper
d e Metering device c Needle Injection valve 7
g Column
Flow cell f Waste
h Waste
472

Capillary LC Accessories
PEEK Coated Fused Silica Capillaries for Use with 100 L/min Flow Range
Item a b c d e f g h From EMPV Flowsensor Injection valve Metering device Injection valve Injection valve Column Detector -switching valve To Flowsensor Injection valve Metering device Needle Column Waste Detector Waste Column Fitting ID (m) Part No. B/B B/C B/C B/B C/D C/D/E E/C/D 100 100 100 100 75 100 75 75 50 G1375-87305 G1375-87306 G1375-87312 G1375-87303 G1375-87311 G1375-87307 G1375-87308 G1315-68708 G1375-87309
Type A P/N 5062-2418
Fittings
Capillaries come with fittings as listed above
Replacement Fittings and Ferrules

Description 1/16 in. fittings, front and back ferrules Nut, SS 1/16 in., 4 mm fittings Ferrule, 1/32 in. with SS lock ring 6 fittings, 2 plugs, PEEK for -valves Double winged nuts and 1/32 in. ferrules 10/pk Unit 10/pk 10/pk 10/pk Type A B B C D Part No. 5062-2418 5063-6593 5065-4423 5065-4410 5065-4422
Type B Nut P/N 5063-6593 Ferrule P/N 5065-4423
LC and LC/MS
Type C P/N 5065-4410
Type D P/N 5065-4422
473

VWD Supplies
Variable Wavelength Detector (VWD)

VWD Replacement Parts
Description Part No. G1314A VW Detector Accessory Kit G1314-68705 Contains: waste accessory, 1 hex key, 2 wrenches, tubing and wrist strap Deuterium Lamp
Variable Wavelength Detector
G1314-60100 5062-8522 Path Length 10 mm 6 mm 5 mm 10 mm Part No. G1314-60086 G1314-60083 G1314-60081 G1314-60082 Cell Volume 14 L 5 L 1 L 14 L
PEEK tubing kit, 400 mm, 0.17 mm id, cell inlet Flow Cell Type Standard Flow Cell Semi-micro Flow Cell Micro Flow Cell Kit High Pressure Flow Cell
Note: A critical parameter of the VWD is backpressure on the cell. Make certain that you use the appropriate capillary from the detector flow cell to waste (P/N 5062-8535).
Typical Column Length 5 cm 10 cm
Typical Peak Width 0.025 0.05 0.1 0.2 Micro Flow Cell
Recommended Flow Cell
Semimicro Flow Cell Standard Flow Cell
LC and LC/MS
20 cm 40 cm
High Pressure Flow Cell For Pressure Above 100 bar
Typical Flow Rate Internal Column Diameter
0.05 0.2 mL/min. 0.2 0.4 mL/min. 0.4 0.8 mL/min. 12 mL/min. 1.0 mm 2.1 mm 3.0 mm 4.6 mm
0.05 5 mL/min.
VWD Flow Cell Selection
474

VWD Flow Cell
G1314-60080 Standard Flow Cell Replacement Parts
Item Description Standard D flow cell kit: Includes 2 windows, 2 gaskets #1, 2 gaskets #2 1 2 3 4 5 6 Cell screw Conical spring Ring, SS Gasket #1, PTFE (10/pk) Quartz windows Gasket #2, PTFE (10/pk) 10/pk 2/pk 10/pk 2/pk 10/pk Unit Part No. G1314-65050 79853-27200 79853-29100 79853-22500 79853-68743 79853-68742 G1314-65051
Standard Flow Cell
G1314-60086 Standard Flow Cell Replacement Parts

Item Description Standard D type flow cell, 10 mm, 14 L, 40 bar Standard D flow cell kit: Includes 2 windows, 2 gaskets #1, 2 gaskets #2 1 2 3 4 5 6 7 Cell screws Conical spring Ring #1, PEEK Gaskets #1, Kapton Quartz windows Gaskets #2, Kapton Ring #2, PEEK 2/pk 10/pk 2/pk 10/pk 2/pk 10/pk 2/pk Unit Part No. G1314-60086 G1314-65061 G1314-65062 79853-29100 G1314-65065 G1314-65063 79853-68742 G1314-65064 G1314-65066
Standard Flow Cell
LC and LC/MS
Note: The cell screw (Item 1) of P/N G1314-60086 is painted black to differentiate flow cell types.
475

VWD Flow Cell
G1314-60083 Semi-micro Flow Cell and Replacement Parts
Item Description Semi-micro flow cell assembly: 6 mm, 5 L, 40 bar Semi-micro flow cell kit, 6 mm, 5 L, 40 bar Includes 2 windows, 4 gaskets: 2 standard #1, 1 semi-micro #1, 1 semi-micro #2 1 2
Semi-micro Flow Cell
Unit
Part No. G1314-60083 G1314-65056
Cell screw Conical spring Ring, SS Quartz windows Gasket #1, PTFE, ordering kit P/N G1314-65056 10/pk 2/pk 2/pk
79853-27200 79853-29100 79853-22500 79853-68742
3 4 5
G1314-60081 Micro Flow Cell and Replacement Parts

Item Description Micro flow cell, 5 mm, 1 L, 40 bar Micro flow cell kit: Includes 2 windows, 2 gaskets #1, 2 gaskets #2 1 2 3 4 5 6 Cell screw Conical spring Ring, SS Quartz windows Gasket #1, PTFE Gasket #2, PTFE Capillary column-detector connector, SS, 400 mm, 0.12 mm id 10/pk 2/pk 2/pk 10/pk 10/pk Unit Part No. G1314-60081 G1314-65052 79853-27200 79853-29100 79853-22500 79853-68742 79853-68743 G1314-65053 5021-1823
LC and LC/MS
Micro Flow Cell
G1314-60082 High Pressure Flow Cell and Replacement Parts

Item Description High pressure flow cell, 10 mm, 14 L, 400 bar High pressure flow cell kit: Includes 2 windows, 2 KAPTON gaskets, 2 PEEK rings 1 2 Cell screw Window, UHP Capillary column-detector connector, SS, 380 mm, 0.17 id 2/pk Unit Part No. G1314-60082 G1314-65054 79853-27200 79853-68734 G1315-87311
High Pressure Flow Cell
476

DAD Supplies
Diode Array Detector (DAD)

Optimal performance through innovative design of the Agilent 1100 Series DAD lamp Lifetime over 2000 hours Precise alignment Thermal stability Easier to handle during installation and removal
DAD Replacement Parts

Four flow cells are available for use with the diode array. Select the flow cell based upon your application.
Flow Cell Path Length (mm) Volume Max Pressure Part No.
Standard Semi micro 500 nL Micro high pressure
10 6 10 6
13 L 8 L 500 nL 1.7 L
120 bar 120 bar 50 bar 400 bar
G1315-60012 G1315-60011 G1315-68714 G1315-60015
A decrease in detector performance or unusual noise levels may mean you have dirty flow cell windows. Clean and reassemble one side of the flow cell before beginning the other side to prevent mixing the front and rear gaskets which have different hole diameters. When you clean or replace flow cell windows, if the washers fall out of the window assembly, they must be inserted in the correct order with a Teflon ring to prevent any leaks from the flow cell window. Clean the cell body with water or isopropanol. After opening the cell you should always use a new gasket.
Typical Column Length 5 cm 10 cm 20 cm 40 cm Typical Peak Width 0.025 0.05 0.1 0.2 0.05-0.2 mL/min. 0.2-0.4 mL/min. 0.4-0.8 mL/min. 0.3-1 mm 2.1 mm 3.0 mm 1-2 mL/min. 4.6 mm 0.05-5 mL/min. 500 nL Flow Cell Semimicro Flow Cell Standard Flow Cell High Pressure Flow Cell Recommended Flow Cell
LC and LC/MS
Typical Flow Rate Internal Column Diameter
DAD Flow Cell Selection
477

DAD Supplies and Flow Cells
G1315A Diode-Array Detector (DAD) Parts and Supplies
Description Deuterium lamp, long life Deuterium lamp, shine through Tungsten lamp Zero dead volume union Holmium oxide filter Lever for holmium oxide filter Capillary, column to detector, 380 mm, 0.17 mm id Capillary, column to detector, 150 mm, 0.12 mm id Unit Part No. 5181-1530 2140-0590 G1103-60001 0100-0900 79880-22711 G1315-45001 G1315-87311 G1315-87312
G1315-60012 Standard Flow Cell and Replacement Parts

Item 1
8 7
Description Standard flow cell, 10 mm, 13 L, 120 bar Window screw Spring washers Compression washer Window holder Quartz window Seal BACK for STD flow cell Seal FRONT for STD flow cell Window assembly (items 1-5) Capillary INLET (0.17 mm, 590 mm long) including heat exchanger Capillary OUTLET (0.17 mm, 200 mm long) Clamp unit Zero dead volume fitting Cell repair kit STD, includes window screw kit, 4 mm hexagonal wrench and seal kit Cell screw kit includes: 2 quartz windows, 2 compression washers, 2 window holders, 2 window screws and 10 spring washers
Unit
Part No. G1315-60012 79883-22402
2
6 5 9 1 10 11 4 3 2
10/pk
5062-8553 79883-28801 79883-22301 1000-0488
3 4 5 6 7 8 9 10 11
12/pk 12/pk
G1315-68711 G1315-68710 G1315-87321 G1315-87302 G1315-84901 0100-0900 G1315-68712 79883-68703
LC and LC/MS
Standard Flow Cell
478

DAD Flow Cells
G1315-60011 Semi-Micro Flow Cell and Replacement Parts
Item 1 2 3 4 5 6 7 8 9 10 11 Description Semi-micro flow cell, 6 mm, 4.5 L, 120 bar Window screw Spring washers Compression washer Window holder Quartz window Seal BACK for semi-micro flow cell Seal FRONT for semi-micro flow cell Window assembly (items 1-5) Capillary INLET (0.12 mm, 290 mm long) including heat exchanger Capillary OUTLET (0.12 mm, 200 mm long) Clamp unit Screw M 2.5, 4 mm long for cell body/clamp Cell repair kit semi-micro, includes window screw kit, 4 mm hexagonal wrench and seal kits Window screw kit, includes 2 quartz windows, 2 compression washers, 2 window holders, 2 window screws and 10 spring washers G1315-87325 G1315-87306 G1315-84901 0515-1056 G1315-68713 79883-68703 12/pk 12/pk 10/pk Unit Part No. G1315-60011 79883-22402 5062-8553 79883-28801 79883-22301 1000-0488 79883-68702 G1315-68710
Semi-micro Flow Cell
G1315-60015 Micro/High Pressure Flow Cell and Replacement Parts
LC and LC/MS
Item 1 2 3 4 5 6 7 8 9
Description Micro/high pressure flow cell, 6 mm, 1.7 L, 400 bar Window assembly, comprises items 2, 3, 4, 5 and 6 Seal ring Quartz window Compression washer Spring washers Window screw Capillary INLET (0.12 mm, 290 mm long) including heat exchanger Capillary OUTLET (0.12 mm, 200 mm long) Clamp unit Screw M 2.5, 4 mm long for cell body/clamp Capillary 0.12 mm, 150 mm long Capillary 0.17 mm, 380 mm long Cell repair kit, includes 1 quartz window, 5 spring washers, 2 seal rings
Unit
Part No. G1315-60015 79883-68703 79883-27101 1000-0953 79883-28802
5 4 2
4 7 9 8
10/pk
5062-8553 79883-22404 G1315-87325 G1315-87306 G1315-84901 0515-1056 G1315-87312 G1315-87311 79883-68700
Micro High Pressure Flow Cell
479

DAD Flow Cells
G1315-68714 500 nL Flow Cell and Replacement Parts
Item Description 500 nL flow cell kit includes: Flow cell assembly, 10 mm, 500 nL, 5 MPa completely assembled, includes items 1, 2, 3, 4, 11, 12, 13, 14, 15, 16 1 Glass-capillary kit inlet, includes: Inlet capillary, 400 mm lg, id 50 m with pre-fixed ferrules (#4) and fitting (#3), plus one PEEK fitting F-120 (#5) Glass-capillary kit outlet, includes: Outlet capillary, 700 mm lg, id 75 m with pre-fixed ferrules (#4) and fitting (#3) Sealing kit, includes items 10, 12 and 7 (Qty=5) 3 4 5 6 7 8 9 10 11 Fitting screw - for 4 mm wrench, QTT=2 Cell ferrules are factory installed PEEK fitting 1/32, not attached to capillaries Upchurch Liteouch ferrules LT-100, (front and back), QTY=4 Union - top - adjustment tool, used for item #7 Union - top - seal, QTY=2 Torque wrench adapter Cell housing 10 mm Cell seal assembly 10 mm Quartz cell body 10 mm Clamp unit Screw M 2.5, 4 mm lg for cell body/clamp Wrench open end 4 mm**
* Part of sealing kit ** Supplied with standard accessory kit P/N G1315-68705
Unit
Part No. G1315-68714
G1315-68703
G1315-68708
G1315-68715 10/pk 5063-6593 5065-4422 10/pk 2/pk 5063-6592 5022-2146 5022-2145 G1315-45003* see kit below G1315-80001 G1315-84901 0515-1056
LC and LC/MS
12 13 14 15
500 nL Flow Cell and Replacement Parts
480

DAD Flow Cells/Fluorescence
Preparative Flow Cells for G1315B DAD and MWD
Description Prep flow cell quartz, 2 MPa Prep flow cell quartz, 2 MPa PTFE tubing, 0.8 mm id PTFE tubing, 0.5 mm id Cell housing Quartz body prep cell Quartz body prep cell Prep flow cell SS, 12 MPa SS capillary Backpressure regulator, 100 psi 0.3 mm 0.06 mm 3 mm 0.5 mm, 250 mm Specs 0.3 mm 0.06 mm 2m 0.8 m Part No. G1315-60017 G1315-60018 G1315-67301 G1315-67302 G1315-27705 G1315-80004 G1315-80003 G1315-60016 G1315-87305 5042-6443
G1321A 1100 Series Fluorescence Detector (FLD)

Description Xenon flash lamp Flow cell Cuvette, kit, 8 L, 20 bar (includes: tubing, SS fitting, front & back ferrule, PEEK fitting, syringe needle, syringe) Cut-off filter kit 389, 408, 450, 500, 550 nm 380, 399, 418, 470, 520 nm 280, 295, 305, 335, 345 nm Corrugated tubing, 6.5 mm id Teflon tubing, 0.7 mm id Fitting male PEEK Capillary column kit to detector, 0.17 mm id, (not assembled) Ferrule front, 1/16 in. SS Ferrule back, 1/16 in. SS Fitting, 1/16 in. SS Screwdriver hexagonal, 100 mm long Screwdriver hexagonal, 100 mm long Calibration sample, glycogen Sample filter, 0.45 m pore size Hex key set 1-5 mm Wrench open end 1/4-5/16 in. 1g 100/pk 3 mm 10/pk 10/pk 10/pk 4 mm 2.5 mm 2/pk 380 mm 5m 5m Unit Specs Part No. 2140-0600 G1321-60005 G1321-60007
5061-3327 5061-3328 5061-3329
LC and LC/MS
5062-2463 5062-2462 0100-1516 G1315-87311 5180-4108 5180-4114 5061-3303 5965-0027 5965-0028 5063-6597 5061-3367 8710-0641 8710-0510
481

RID Detector/Test Solutions
G1362A 1100 Series Refractive Index Detector (RID)
Description Tubing kit, includes: 300 mm recycle valve to recycle port, 200 mm recycle valve to waste port, 120 mm purge valve to recycle valve, 270 mm purge valve to sample cell, 170 mm purge valve to reference cell Interface tubing kit, includes ferrule 1/8 in., nut 1/3 in., Teflon tubing Interfacing capillary, 400 mm, 0.17 mm id Restriction capillary, 3000 mm, 0.17 mm id 400 mm Specs Part No. G1362-68709
G1362-68706 G1362-87300
3000 mm G1362-87301
1100 Series LC Test Solutions

OQ/PV Test Solutions
Description Standard caffeine OQ/PV solution: 5.0, 25.0, 125.0, 250.0, 500 g/mL caffeine in water LC/MS caffeine OQ/PV solution: 0.5, 1.0, 5.0, 25.0, 50.0 g/mL caffeine in water Micro LC caffeine OQ/PV solution: 2.0, 4.0, 20.0, 100.0, 200.0 g/mL caffeine in water RID OQ/PV solution: 5, 10, 15, 25, 50 mg/mL glycerin in water Isocratic/gradient standard (0.15% dimethylpthalate, 0.15% diethyl-phthalate, 0.01% biphenyl, 0.003% O-terphenyl in MeOH [w/w]). The gradient standard includes 0.32% dioctyl phthalate as well. Two 0.5 mL ampoules of each. Isocratic standard as above. One 0.5 mL ampoule
Part No. 8500-6762 8500-6917 5065-4420 5064-8220 01080-68702
LC and LC/MS
01080-68704
482

LC/MS Supplies
LC/MS Supplies
Agilent 1100 Series LC/MSD and LC/MS Trap Supplies
Instrument Supplies
Description EM replacement horn LC/MS nitrogen gas purifier, 1/4 in. fitting Rough pump oil, Inland 45 API-ES nebulizer needle kit APCI nebulizer needle Nebulizer needle ferrule Corona needle Clean cloth Abrasive mesh (micro-grit paper) Precision glass capillary for G1946B and G1946C Precision glass capillary with platinum ends for G1946D Inlet inline filter assembly for LC/MSD Replacement frits for P/N G1946-60180 High pressure semi-prep filter (inline mixer for LC/MSD) SS frits for P/N 5022-2165 10 m 10/pk 5 m 5/pk 15/pk 1 liter Specs Unit Part No. 05971-80103 G1946-80047 6040-0834 G2427A G2428A G1946-20213 G1947-20029 05980-60051 8660-0852 G1946-80009 59987-20040 G1946-60180 0100-2051 5022-2165 5022-2166
LC and LC/MS
Helpful Hint: Nebulizer needle problems can cause low signal. Be sure to inspect and change your nebulizer needle at regular intervals.
Chemical Supplies*
Description Electrospray calibrant solution APCI/APPI calibrant solution Unit 100 mL 100 mL Specs Part No. G2421A G2432A G2423A G2424A
ES/APCI positive ion 5 x 1 mL ampoules Reserpine, 5 ng/L in IPA: H2O performance standard 50:50 (mol. wt. 609.28) ES negative ion 5 x 1 mL ampoules Acid Red 4, 10 ng/L in IPA: H2O performance standard 50:50 (mol. wt. 357.1)
APCI negative ion 5 x 1 mL ampoules 4-Nitrophenol, 10 ng/L in IPA: H2O G2425A performance standard 50:50 (mol. wt. 138.08) Myoglobin standard Caffeine standards kit for LC/MS Electrospray tuning mix for Esquire LC 5 x 5 mL ampoules 0.5, 1.0, 5.0, 25 and 50 g/mL in H2O G2426A 8500-6917 G2431A
Helpful Hint: Our calibrants and standards are designed to be a perfect fit for tuning your 1100 Series LC/MSD and maintaining ultimate performance.
* To keep your LC/MSD running at peak efficiency, Agilent offers a complete selection of calibrants and standards for both Electrospray and APCI applications.
483

LC/MS Supplies Agilent 59987 ElectroSpray Interface
59987 ElectroSpray Interface Supplies
Description Union, U-435 PEEK tubing, 0.0025 in. id PEEK tubing, 0.03 in. id 100 L syringe Inner needle assembly Part No. 0100-1619 0890-1901 0890-1921 5182-9610 59987-60026
Agilent 59980B Particle Beam

59980B Particle Beam Supplies
Description Capillary ferrule Frits Fused silica capillary, 79 mm Oil filter demister Momentum Separator Plastic Parts Kit (nozzle, skimmer 1, skimmer 2) Vacuum pump fluid, (Krytox) Particle beam demo sample 1 qt 10/pk 5/pk Unit Part No. 0100-1443 3150-0559 59980-20133 1535-4970 59980-60104 6040-0730 8500-5275
LC and LC/MS
Agilent 59883B ThermoSpray

59883B ThermoSpray Supplies
Description Thermospray Cartridge Thermospray Probe Seal, Vespel Part No. 05989-60172 05988-20242
484

Pump Accessories
Agilent Technologies 1050 Parts and Supplies

79851A/52 Pump Module Parts and Supplies
Description 1050 maintenance CD-ROM 1050 pump maintenance kit: includes 5 outlet caps, 5 gold outlet seals, 1 cartridge for active inlet valve, 10 Teflon frits, 4 piston seals Outlet caps Outlet gold seal PTFE frits Piston seals, graphite-filled Teflon Active inlet valve Cartridge for active inlet valve Pump seal, polyethylene (for normal phase (Hexane) applications) Sapphire piston (plunger) Velocity regulator Seal wash kit: Includes 2 film washers, 4 m flexible tubing, 2 seals, cable strap, hose clamp, 2 support rings, 5 mL luer lock syringe, adapter Wash seal Insert tool for wash seal Gasket, wash seal Purge valve Replacement purge valve assembly Outlet ball valve, Titanium and standard pump Frit adapter assembly Solvent reservoir, 1 liter Helium sparger assembly (filter, connector) Glass filter, 10-16 m pore size (for helium) Connector for helium sparger Solvent inlet filter assembly 10 m, SS* Solvent inlet filter 10 m, SS Bottle head assembly Sucking tube, includes filter and fitting Tube bushing, Teflon (for bottle head assembly tube) Tube screw (for bottle head assembly tube) Solvent tubing, FEP, 5 m, 1.5 mm id, 3 mm od Helium tubing, 5 m, PTFE, 0.7 mm id Polyethylene actuator air tubing, per meter 6/pk 6/pk 3/pk 2/pk 5/pk 2/pk 4/pk Unit Part No. 01050-60000 01018-68724
1050 Maintenance Kit P/N 01018-68724
5062-2485 5001-3707 01018-22707 5063-6589 01018-60010 5062-8562 0905-1420 5063-6586 5062-2486 01018-68722
Bottle Head Assembly P/N 01018-60017
0905-1175 01018-23702 5062-2484 01018-68723 G1311-60009 G1311-60012 01018-60007 9301-0656 01019-82702 5041-8339 5062-8515 01018-82701 01018-60025 01018-60017 01018-67303 79835-21734 5041-2163 5062-2483 5062-2462 5021-7127
* Includes P/N 01018-60025, 5041-8339, 5062-8515 (1 connector)
Glass Filter and Helium Sparge Connector P/N 5041-8339, P/N 5062-8515
LC and LC/MS
Solvent Inlet Filter P/N 01018-60028
485

Degasser/Flow Connectors/Sample Introduction Supplies
G1303 Online Degasser Parts and Supplies
Description Online degasser accessory kit: Includes: bottle head assemblies, nuts and ferrules, disposable syringe, syringe adapter, tubing, inlet filter Bottle head assembly Ferrules Male nuts Disposable syringes, luer, 20 mL Syringe adapter, 1/16 in. and 2 in. needle Glass filter, solvent inlet Frit adapter, 3 mm Solvent tubing, 5 m, FEP, 1.5 mm id, 3 mm od 4/pk 8/pk 4/pk 10/pk Unit Part No. G1303-60001
01018-60017 5062-8519 5062-8518 5062-8534 9301-1337 5041-2168 5062-8517 5062-2483
1050 Module Flow Connectors

From Pump Pump Injection valve Autosampler To Rheodyne valve Universal Column Column Detector Waste Waste Description 70 cm, 0.25 mm id capillary 70 cm, 0.25 mm id capillary, 2 standard fittings 40 cm, 0.17 mm id capillary 80 cm, 0.17 mm id capillary 60 cm, 0.17 mm id capillary 0.7 mm id Teflon tubing (5 m) Corrugated tubing (5 m) Part No. 01078-87306 01018-67305 79826-87608 01078-87305 01048-87302 5062-2462 5062-2463
Pump/Injector/Detector
LC and LC/MS
Column Detector Pump
Connections for Ti Series
Pump Pump Injector valve Needle Seat
Rheodyne valve Universal Column Rheodyne valve
70 cm, 0.25 mm id capillary, Ta 01079-87306 70 cm, 0.25 mm id capillary, Ta 01019-67305 35 cm, 0.17 mm id capillary, Ta 01019-87608 01078-87303
Manual Injector Valve, Parts and Supplies

Description Rheodyne valve 7125 Rotor seal, Tefzel, for extended pH range Rotor seal, Vespel Stator face assembly for Rheodyne valve 7125 Syringe, 25 L, removable needle Part No. 0101-0607 0101-0620 0101-0623 0101-0624 5182-9719
486

Sample Introduction Supplies
Manual Injector Sample Loops, Part and Supplies
Description 10 L 20 L 50 L 100 L 500 L 1 mL 2 mL
PEEK Valve Supplies
Part No. 0101-0376 0101-0377 0101-0378 0101-0379 0101-1251 0101-1219 0101-1250
Description 500 L safety loop Rotor seal, Tefzel for extended pH range Stator face assembly, SS Stator, PEEK Isolation seal
Part No. 0101-0727 1535-5084 1535-5083 1535-5082 1535-4046
79855A Autosampler Supplies

Description 1050 Autosampler Maintenance kit: Includes 2 needle seats, 2 needles, seat capillary, Vespel rotor seal Needle seat Needle assembly Seat capillary Rotor seal, Vespel Rotor seal, Tefzel for extended pH range Expanded injection volume kit: Includes 500 L and 2 mL seat capillaries, flush union 500 L seat capillary 2 mL seat capillary Flush union Piston seal 2 mL crimp top vial kit: Includes 2 mL vials (100/pk), crimp caps (100/pk), 11 mm crimper 21 position vial tray 34 position vial tray 2/pk Unit Part No. 01078-68721
LC and LC/MS
79846-67101 01078-67200 01078-87303 0101-0626 0101-0627 01078-68704 01078-87307 01078-87308 79846-23203 5063-6589 01078-68705 01078-44501 01078-44511
487

Microvials for 1050 Autosampler
Description Crimp cap microvial with fixed insert Crimp clear microvial, tapered* Crimpl clear microvial, round bottom* Crimp amber microvial round bottom** 8 mm crimp cap with PTFE/silicone septa Sleeve for HP 1050 Unit 100/pk 500/pk 500/pk Volume (mL) 0.2 0.1 0.3 0.5 0.7 Cap Size (mm) 11 8 8 8 8 8 Part No. 9301-1388 5180-0844 5180-0841 5180-0806 5180-0805 5180-0842 5021-1859
*Need sleeves P/N 5021-1859 and 21 position tray **Work directly with 34 position tray
Crimp amber microvial tapered** 500/pk 500/pk 500/pk 25/pk
Manual Injector Parts

Description Rotor seal, Tefzel Stator, Titanium Part No. 0101-0620 0101-0663
Autosampler Parts and Supplies-Ti Series
LC and LC/MS
Description 1050 Autosampler maintenance kit: Includes Titanium needle, Ti/PEEK needle seat, Ta seat capillary, Vespel rotor seal Needle, Titanium Needle seat, Titanium/PEEK Seat capillary, Tantalum Rotor seal Seat capillary, Tantalum, 0.5 mL Seat capillary, Tantalum, 2 mL Zero dead-volume union, Titanium
Part No. 01079-68721 01079-67201 01079-67101 01079-87303 0101-0627 01079-87307 01079-87308 5021-1871
Note: Titanium Manual Injection Loops on page 453.
488

VWD Supplies
79853C Variable Wavelength Detector (VWD) Supplies

79853C Variable Wavelength Detector (VWD) Supplies
Description Deuterium lamp (1050 and 1090, DAD, MWD, VWD) PEEK inlet tubing assembly, 40 cm, .007 in. id, 1/16 in. od PEEK/Teflon outlet tubing assembly, 0.25 mm id 1/16 in. od, joined by PEEK union, 48 cm Standard flow cell, Titanium Part No. 79883-60002 5062-8522 5062-8535 79853-60011
VWD Standard Flow Cell

Item Description Standard cell kit (SS and Titanium versions): Includes 2 windows, 2 gaskets #1, 1 gasket #2, 1 gasket #3 1 2 3 4 5 6 7 Cell screw Conical spring Ring, stainless steel Gasket #1, Teflon Quartz window Gasket #2, Teflon Gasket #3, Teflon 10/pk 2/pk 10/pk 2/pk 5/pk 5/pk Unit Part No. 79853-68718
1 2 2 2 3 4 6 7
7 5 4 3
79853-27201 79853-27203 79853-27202 79853-68720 79853-68719 79853-68721
2 2 2 1
Standard Flow Cell
LC and LC/MS
79853-68722
VWD Standard Flow Cell D Version

Item Description Unit Part No. 79853-60000 79853-68741 79853-27200 10/pk 79853-29100 2/pk 2/pk 5/pk 5/pk 79853-22500 79853-68742 79853-68744 79853-68745
Standard Flow Cell D Version
Standard flow cellD version (for serial numbers 3522J04305 or for detectors with replaced optical units) Standard cell kitD version: Includes 2 windows, 2 gaskets #1, 1 gasket #2, 1 gasket #3 1 2 3 4 5 6 7 Cell screw Conical spring, D version, Ring, stainless steel, D version Gasket #1, D version, Teflon Window, D version Gasket #2, Teflon, D version Gasket #3, Teflon, D version
10/pk 79853-68743
489

VWD Supplies
Micro Flow Cell
Item Description Micro flow cell, stainless steel, 4 L Micro cell kit: Includes 2 standard windows, 2 gaskets #1, 2 micro Teflon gaskets 1 2 3
Micro Flow Cell
Unit
Part No. 79853-60010 79853-68723 79853-27201
Cell screw Conical spring Ring, stainless steel Gasket #1, Teflon Quartz window Gasket #2 micro, Teflon Flexible capillary, 0.12 x 400 mm, no fittings PEEK fingertight fittings 2/pk 10/pk 2/pk 10/pk 2/pk 10/pk
79853-27203 79853-27202 79853-68720 79853-68719 79853-68724 5021-1823 0100-1516
4 5 6
High-Pressure Flow Cell

Description High pressure flow cell kit (for 79853-60009): Includes 2 windows, 2 polyimide rings, 2 gaskets #1, 1 gasket #2 Quartz window 2/pk Unit Part No. 79853-68728 79853-68714
Ultra-High-Pressure Cell
Item Description Ultra-High-pressure cell, 400 bar UHP cell kit: Includes 2 windows, 2 gaskets #1, 1 gasket #2, UHP PEEK ring 1 2 3 Cell screw UHP quartz window UHP gasket #2 2/pk 2/pk Unit Part No. 79853-60013 79853-68733 79853-27200 79853-68734 79853-68738
LC and LC/MS
Ultra-High Pressure Flow Cell
Preparatory Flow Cell

Description Preparatory flow cell, Titanium Gasket #1, preparatory, Teflon Unit Part No. 79853-60012 79853-68720
490

MWD/DAD Supplies
79854A Multiple Wavelength Detector (MWD)/G1306A Diode-Array Detector (DAD) Parts and Supplies
Description Deuterium lamp Standard Flow cell, Series II Standard Flow cell, Series II Gaskets (not for high-pressure cells) High-pressure flow cell, SS High-pressure micro-flow cell, SS Detector inlet capillary Detector outlet capillary Detector inlet capillary Detector outlet capillary Standard cell repair kit: Includes 12 gaskets, 2 window holders assembled with windows and washers, 2 cell screws, 10 washers, hex key Cell screw kit: Includes 2 window holders assembled with windows and washers Gaskets Window, quartz Window holder Window screw Compression washer Hex key High-pressure cell kit: Includes 1 window, 5 washers, 2 seal rings High pressure window, conical High pressure seal ring Slit assembly Slit assembly Slit assembly Specs 6 mm path length, 8 L, 120 bar 10 mm path length, 13 L, 120 bar 12/pk 10 mm path length, 13 L, 400 bar 6 mm path length, 1.7 L, 400 bar 0.17 mm id, 10 cm 0.17 mm id, 15 cm 0.12 mm id, 10 cm 0.12 mm id, 39 cm Unit Part No. 79883-60002 79883-60051 79883-60052 79883-68702 79883-60054 79883-60055 79883-87303 79883-87304 79883-87305 79883-87306 79883-68701
79883-68703 12/pk 79883-68702 1000-0488 79883-22301 79883-22402 79883-28801 8710-2023 79883-68700 1000-0953 79883-27101 79883-80002 79883-80004 79883-80008
6 mm
LC and LC/MS
2 nm 4 nm 8 nm
491

General Supplies
Agilent 1090 Parts and Supplies

General Parts and Supplies
Description Stainless steel fitting, high pressure, blanking nut 1/6 in. Polyethylene actuator air tubing, 1/4 in. od (per meter) Teflon general purpose tubing, 1/8 in. od (per meter) Filters
1090 Liquid Chromatograph
Unit
Part No. 01080-83202 5021-7127 5021-7113 3150-0942 3150-0463 3150-0462
Helium inlet Small dust filter for 1090 mainframe Large air filter for 1090 mainframe Syringes Disposable syringe, 20 mL, luer Zero Dead-Volume Fittings Union, 1/16 x 1/16 in. Tee, 1/16 x 1/16 x 1/16 in. Standard tool kit: includes 1/4 in. and 5/16 in. wrenches, (2/ea); 1/2 in. and 7/16 in. wrench; 3 in. shaft Pozidriv screwdriver; 4 in. shaft Pozidriv screwdriver hexagonal key set, isocratic test sample, tubing, fuses, syringe Hexagonal key set (metric) Wrench, 1/4 in. and 5/16 in. 2/ea Wrench, 1/2 in. and 7/16 in. Pozidriv screwdriver, 3 in. shaft, #1 pt, fits #2-4 screws Pozidriv screwdriver, 4 in. shaft, #2 pt, fits #5-10 screws 10/pk
5062-8534 0100-0900 0100-0969 01090-68721
8710-0641 8710-0510 8710-0806 8710-0899 8710-0900
LC and LC/MS
Pump and Flow Path Supplies

Description Kalrez O-ring, for solvent reservoir Solvent filter/helium Sparger assembly with tubing Solvent filter, SS, 10 m Glass frit for helium sparging, 10-16 m pore size Teflon tubing and glass frit for helium sparging Solvent inlet filter, for external channel only, stainless steel Filter assembly, for external channel, bottle head, filters, and tubing Solvent reservoir, 1 liter capacity, neck 29/32 Replacement sieve, for high-pressure pump check-valve cartridge Replacement sieve, for extended pH (12.5) use Check-valve housing Part No. 0905-1259 01090-82701 01090-60008 5041-8339 01090-60009 01018-60025 01090-60006 9301-0656 79835-65213 79835-65216 79835-25211
492

Pump and Flow Path Supplies (continued)
Item 1 2 3 4 5 6 7 8 9 Description Ball valve cartridge Ball valve cartridge, to pH 12.5 Capillary Low-pressure tube Helium tubing Connector for helium sparger Helium sparger, glass filter Helium sparger with adapter Solvent capillary Solvent filter frit Loop capillaries 25 L 125 L 250 L 10 11 12 13 14 15 ZDV-union Needle insert Needle seat Seat capillary Syringe capillary Syringe Syringe seal 16 Waste sleeve O-ring 17 18 19 20 21 22 23 24 25 Waste tube Oven heat exchanger (also known as heater assembly) Capillary for unheated column compartment Flush connector cap Union, 1/16 in., SS Detector capillary Flush valve Restriction capillary Flexible waste tubing .102 in. od, .058 in. id, .02 in. wall 0.17 mm id, 370 mm 0.12 mm id, 370 mm 190 mm, 0.6 mm id 0.6 mm id 0.6 mm id 0.12 mm id, 105 mm 0.17 mm id, 105 mm 110 mm, 0.25 m id 25 L 250 L 25 L 250 L 25 L 250 L 4 mm id, 4.8 mm od 25 L 250 L 10 L 10-16 m pore size 130 mm, 0.25 mm id 130 mm, 0.6 mm id 5m 6/pk Specs Unit Part No. 79835-67101 79835-67102 79835-67308 79835-67307 5062-2461 5062-8515 5041-8339 01019-82702 01090-87606 01090-60008 79846-87604 79846-87612 79846-87613 0100-0900 79846-87201 79846-87202 79846-67101 79846-87605 01090-87303 79846-87601 9301-0633 9301-0678 79846-27101 79846-27103 79846-24501 79846-24502 0905-0970 79846-27303 01090-61105 01090-87317 01090-87301 01090-87615 0100-1017 79846-87609 79846-67902 79846-87600 5021-7113
LC and LC
493

Pump and Flow Path Supplies (continued)
Item 26 27 28 29 30 31 Description Waste capillary Delivery tube A Delivery tube B Delivery tube C Connection capillary Connection capillary Pump seal, black Vespel Pump seal, orange (use for buffered solutions) Sapphire piston Diaphragm for high pressure solvent delivery system High pressure pump head High pressure pump head compression screw (must be replaced with diaphragm) High pressure torque wrench (for compression screws) High pressure pump hydraulic fluid Spare part kit for 1090 solvent delivery system: Includes 100 mL hydraulic fluid, filter assembly, diaphragm, sieve assemblies (2/pk), ball valve cartridges (2/pk), sapphire plunger, low pressure fittings seals (2/pk) Static Solvent Mixer Replacement frits for solvent mixer 40 L pore size Mounting Kit: For installation of solvent mixer in 1090 includes: connecting capillary (320 mm long, 0.25 mm id with pre-mounted fittings) 6/pk 100 mL Specs 0.6 mm id 0.6 mm id, 600 mm 0.6 mm id, 500 mm 0.6 mm id, 430 mm 0.9 mm id, 290 mm 0.6 mm id, 90 mm 4/pk Unit Part No. 79846-87610 79835-67302 79835-67305 79835-67306 01090-87603 79835-67304 5062-2494 0905-1194 3980-0672 79835-04123 79835-27717 0515-0502 8710-1158 01010-80002 79835-68701
79835-87330 79835-62701 01090-68704
LC and LC/MS
1090 Flow Path
494

Variable Volume Auto Injector Supplies
Description Sample loop, standard Sample loop Sample loop Needle seat Seat capillary Seat capillary Capillary, syringe to injection valve Rotor seal, Tefzel Rotor seal, Vespel 25 L Variable Volume Injector Syringe Syringe Replacement plunger for syringe Small seal at threaded end of syringe, Teflon Needle assembly, standard Waste sleeve O-ring for waste sleeve Spare parts kit for 25 L variable volume injector: Includes 2 syringe seals, syringe plunger, rotor seal, 2 needle assemblies, 2 needle seats, 0.12 mm id seat capillary, wrench 250 L Variable Volume Injector Syringe Supplies 250 L Syringe Upgrade Kit: Includes parts to convert the maximum injection volume of the variable volume injector from 25 L to 250 L, waste sleeve, needle, restriction capillary, 250 L loop capillary 250 L Variable Volume Injector Syringe Replacement plunger for syringe Small seal at threaded end of syringe, Teflon Needle assembly, grooved Waste sleeve for syringe O-ring for waste sleeve 4.48 mm id 79846-68701 4.48 mm id 25 L 9301-0633 9301-0675 79846-27101 79846-87201 79846-24501 0905-0970 79846-68702 0.12 mm id, 110 mm 0.17 mm id 0.12 mm id, 25 m pH up to 12.5 Specs 25 L 125 L 250 L Part No. 79846-87604 79846-87612 79846-87613 79846-67101 79846-87605 01090-87303 79846-87601 1535-4900 1535-4048
Spare parts kit for Variable Volume Injector P/N 79846-68702
LC and LC/MS
9301-0678 9301-0677 79846-27103 79846-87202 79846-24502 0905-0970
Autosampler Parts and Supplies

Description Magazine for 1090 automatic sampler Extraction tool (to remove vials from 1090 magazine) Part No. 79847-60005 79847-44901
495

Microvials for 1090 Autosampler
Description Crimp cap microvial with fixed insert Crimp clear microvials, tapered* Crimp clear microvials, round bottom* Sleeves for HP 1090, black Volume Cap Size (mL) (mm) Unit 11 8 8 0.2 0.1 0.3 100/pk 500/pk 500/pk 500/pk 10/pk Part No. 9301-1388 5180-0844 5180-0841 5180-0842 5180-0843
* Need sleeves P/N 5180-0843
8 mm crimp caps with PTFE/silicone septa 8
7010/7125 Rheodyne Valve Sample Loops (3/16 in. SS)

Description Sample volume: Specs 5 L 10 L 20 L 50 L 100 L 200 L 500 L 1 mL* 2 mL* 7010 Rotor seal, Vespel, 3 grooves 7010 Rotor seal, Tefzel (up to pH 12.5), 3 grooves 7125 Rotor seal, Vespel, 2 grooves 7125 Rotor seal, Tefzel, 2 grooves 7413 Rotor seal, micro valve, Vespel 6-port manual injection valve, Rheodyne 7125* Unit Part No. 1535-4860 0101-0376 0101-0377 0101-0378 0101-0379 0101-1252 0101-0282 0101-1219 0101-1250 1535-4048 1535-4900 0101-0623 0101-0620 1535-4861 0101-0607
* These loops come without ferrules and nuts.
LC and LC/MS
Column Switching Valve Parts and Supplies

Description 0.12 mm id Capillary kit (Capillaries are pre-swaged with valve fittings (long nose) on one end, and standard fittings on the other): Includes one 400 mm, three 280 mm, three 80 mm, one 280 mm both ends with standard fittings, ZDV union. 0.17-mm id Capillary kit (Capillaries are pre-swaged with valve fittings (long nose) on one end, and standard fittings on the other): Includes one 400 mm, three 280 mm, three 80 mm, one 280 mm both ends with standard fittings, ZDV union. Column bypass flexible capillary, 280 mm, 0.12 mm id Flexible capillary, valve connection, 80 mm, 0.12 mm id Part No. 79826-68701
79826-68702
01090-87610 79826-87601
496

Sample Introduction Supplies/FPD Accessories
Column Switching Valve Parts and Supplies (continued)
Description Capillary, valve connection Capillary, valve connection Column bypass capillary Capillary, valve connection Capillary, valve connection Capillary, valve connection Union, zero dead-volume fitting 7010 Rotor seal, Vespel Specs 280 mm, 0.12 mm id 400 mm, 0.12 mm id 280 mm, 0.17 mm id 80 mm, 0.17 mm id 280 mm, 0.17 mm id 400 mm, 0.17 mm id Part No. 79826-87602 79826-87605 01090-87304 79826-87606 79826-87607 79826-87608 0100-0900 1535-4048
79881A Filter Photometric Detector (FPD) Parts and Supplies

Description Deuterium lamp Outlet capillary 1090 spare parts kit for FPD: Includes 10 cell springs, 5 0.6 mm cell gaskets, 5 1.2 mm cell gaskets, 3 cell windows, 0.12 mm capillary, smooth point tweezers, 4 mm wrench Cell spring Cell gasket Cell gasket Cell window Capillary Tweezers, smooth point Air filter Filter Filter Filter Filter Filter 210 nm 254 nm 269 nm 280 nm 340 nm 0.12 mm id, 1 m 4 3/4 in. long 0.6 mm 1.2 mm 0.25 mm id, 1 m Specs Part No. 79880-60002 79881-67302 79881-68701
79881-09103 79881-27101 79881-27102
LC and LC/MS
79881-28101 79881-67301 8710-0007 01090-07101 79881-62761 79881-62763 79881-62769 79881-62764 79881-62765
497

DAD Supplies
Diode-Array Detector (DAD) Series I Parts and Supplies
Description Deuterium lamp Connecting capillary, ZDV Heat exchanger, equipped with female connectors, ZDV Heat exchange insulation kit Replacement heat exchanger Air filter 0.12 mm id capillary 380 mm, 0.12 mm id, 1/16 in. male 0.17 mm id capillary* , 1/16 in. Specs Part No. 79883-60002 79880-67303 01090-87307 01090-68701 79880-67304 01090-07101
* Needs insulation kit (P/N 01090-68707) if no heat exchanger was installed.
Diode-Array Detector (DAD) Series II Parts and Supplies

Description Deuterium lamp Flow cell Flow cell Cell gaskets Cell repair kit: includes gaskets 2 windows, 2 window holders, 2 window screws, 2 compression washers Window Window holder 6 mm path length, 8 L 10 mm path length, 13 L 12/pk 12/pk Specs Unit Part No. 79883-60002 79883-60091 79883-60092 79883-68702 79883-68701
1000-0488 79883-22301 79883-22402 79883-28801 79883-60055 2/pk 79883-68700
LC and LC/MS
Window screw Compression washer High-pressure micro-flow cell 6 mm path length, 1.7 L High-pressure cell kit: includes window, washers (5/pk), seals 1090 DAD slit assembly 1090 DAD slit assembly 1090 DAD slit assembly 1090 DAD inlet capillary 1090 DAD outlet capillary 1090 DAD inlet capillary 1090 DAD outlet capillary Heat exchanger Heat exchanger 2 nm 4 nm 8 nm 0.12 mm id, 10 cm 0.12 mm id, 37 cm 0.17 mm id, 10 cm 0.17 mm id, 37 cm 0.12 mm id, 1/16 in. female, 1/16 in. ZDV 0.17 mm id, 1/16 in. female, 1/16 in. ZDV
79883-80022 79883-80024 79883-80028 79883-87305 79883-87306 79883-87322 01090-87322 79880-67304 01090-87307
498

Detector Module Supplies
1040 Detector Module Supplies

Diode-Array Detector (DAD) 1040 Series Detector Module Supplies
Description Inlet capillary Flow cell Flow cell Inlet capillary Heat exchanger Outlet capillary Detector lamp assembly Inlet capillary 880 mm x 0.12 mm id, rigid Unit 01046-60010 79881-67301 79881-67302 01046-68702 Specs 880 mm x 0.12 mm id 6 mm path length, 8 L 10 mm, 13 L 0.12 mm id, 50 cm 0.12 mm id 0.17 mm id, 37 cm Part No. 01040-87602 79883-60041 79883-60042 79883-87328 79880-67304 79883-87322 01037-60002 01040-87602
1040 Diode-Array Detector Series II
1037/47A Refractive Index Detector
1046 Programmable Fluorescence Detector Flow cell Inlet capillary for flow cell Outlet capillary for flow cell Spare parts kit for flow cell: includes 10 gasket stepwindows, 10 gasket cells, 10 guide rings, 3 stepwindows, 2 cell rings, stepwindow black, 10 guide rings, tweezers, toothpicks Gaskets, stepwindow Gasket, cell Stepwindow Stepwindow, black Cell chamber Guide ring Xenon arc flash lamp Cutoff filter Filter kit: five filters Filter kit: five filters Filter kit: five filters 295 nm 389 nm, 408 nm, 450 nm, 500 nm, 550 nm 380 nm, 399 nm, 418 nm, 470 nm, 520 nm 280 nm, 295 nm, 305 nm, 335 nm, 345 nm 5 L
LC and LC/MS
10/pk 10/pk
01046-67101 01046-67102 01046-28101 01046-28103 01046-28102
5/pk
01046-67103 2140-0549 5062-8530 5061-3327 5061-3328 5061-3329
499

Detector Module Supplies
1046 Programmable Fluorescence Detector (continued)
Description Slit Slit Slit Specs 1 mm 2 mm 4 mm Unit Part No. 01046-60021 01046-60022 01046-60023
Agilent 1049 Electrochemical Detector Parts and Supplies Item Description 1 Working electrode: Glassy carbon Platinum
3
Specs
Unit
Part No. 01049-64105 01049-28801 01049-28802 01049-60013
Gold
4 10 4b 9 8 5 4a
2 3 4
Working electrode assembly a. Silicone tubing Electrode swivel nut Solid state reference electrode a. Internal electrolyte electrode b. Seal (not shown) 5/pk
5062-2474 01049-25701 01049-62901 01049-62902 01049-47102 01049-27708 01049-45201
7 11 6
1 2a 2
5 6 7 8 9 10 11
Cell body black Electrode socket FEP spacer Connector auxiliary electrode Inlet tubing, red Teflon Inlet connection nut and ferrule Outlet tubing, blue Teflon KCl electrolyte, aqueous solution Polishing kit includes: abrasive paper and polishing solution Mounting clamp Inlet capillary PEEK tubing length, orange PEEK finger tight fittings Complete ECD cell 80 cm, 0.17 mm id 1/16 in. od, 0.5 mm id, 1.5 m 2/pk 0.5 m 0.5 m 10/pk
01049-24705 01049-27604 01049-27305 5021-1867 01049-27306 01049-92101 01049-67001 5021-1866 01048-87302 0890-1761 0100-1516 01049-68700
LC and LC/MS
Electrochemical Detector Cell
500
LC and LC/MS Columns

Column Selection

Column Selection
To use the column selection guide diagram below, simply follow the path for your analyte and mobile phase. At the far right, follow your final column selection to the catalog section pages indicated.
Hexane Soluble Organic Solvent Soluble Molecular Weight <2000 MeOH and MeOH:H20 or ACN and ACN:H20 Soluble THF Soluble
Normal-Phase Mode with Bare Silica Normal-Phase Mode with Bonded Phase Reversed-Phase Mode with Bonded Phase Gel-Permeation (Small Molecule)
Pg. 573
Pg. 573
Pg. 522
Pg. 577 Pg. 522 Pg. 522 Pg. 522 Pg. 563
SAMPLE Water Soluble
Non-Ionic
Reversed-Phase Mode with Bonded Phase Reversed-Phase Mode with Bonded Phase Using Ionization Control Reversed-Phase Mode with Bonded Phase Using Ion-Pair Agent Reversed-Phase Mode with Bare Silica Ion-Exchange Mode
Ionic
LC and LC/MS
Pg. 590 Pg. 577 Pg. 585 Pg. 590 Pg. 532
Organic Solvent Soluble Molecular Weight >2000 Water Soluble
Gel-Permeation Chromatography Gel-Filtration Chromatography Ion-Exchange Mode with Wide-Pore Material Reversed-Phase Mode with Wide-Pore Material
Adapted with permission from Practical HPLC Methodology and Applications, Brian A. Bidlingmeyer, John Wiley & Sons, Inc., New York, p. 109
501

Column Selection
Column and Mobile Phase Guidelines: Reversed Phase

HPLC columns consist of two parts, the column chemistry and hardware. For the proper column chemistry, consult the catalog section for each type of bonded phase. For choosing column hardware and particle sizes, consult the section on column sizes and rapid separations, including Rapid Resolution columns and new CombiHT columns.
HPLC Column
Stationary Phase Type of Surface Particle Size
Column Dimensions Inner Diameter
Pore Size
Length
Chemical Properties
Chemical Lifetime/Selectivity Retention Factor
Physical Properties
Efficiency Speed
1. Size Selection. Choose a column packing with small pore (60-80) if the solute molecular weight is less than about 4000. Otherwise, use column packing with 300 pore size. 2. Particle Size Selection. The standard particle for HPLC is 5 m. If high-speed (faster than 5 minutes/run) analyses are required, packing with 3.0 or 3.5 m particles in shorter columns can produce faster high-resolution separations (see Column Hardware Section). Many products that use 3 m packing use 0.5 m frits that plug easily. However, using ZORBAX Rapid Resolution columns (3.5 m particles with a narrow particle-size distribution, no fines, and 2 m frits) offers relief from plugging and permits use of standard equipment while reducing run times. 3. Column Configuration. The column configuration most often recommended for analytical method development is 4.6 x 150 mm. If more resolution is needed, use a longer column, 4.6 x 250 mm. After method development, choose the column internal diameter (e.g., 2.1, 3.0 mm) to accommodate additional application objectives (e.g., sensitivity, solvent usage).
LC and LC/MS
502

Column Selection
4. Silica Type and Bonded Phase A) Silica Type: ZORBAX reversed-phase columns use two different types of porous silica microspheres, the original ZORBAX SIL and ZORBAX Rx-SIL. ZORBAX Rx-SIL is a more highly purified, less acidic silica than the original ZORBAX SIL. Increased acidity means a larger potential for interaction between the analyte and silanol groups on the silica surface, especially if the solutes are basic. If doing research or method development on a new procedure, we strongly recommend using reversed-phase products based on ZORBAX Rx-SIL. However, many excellent methods have been developed on reversed phase columns based on ZORBAX SIL and we continue to manufacture these high quality, reliable products. B) Bonded Phase: A good first choice for bonded phase is C8 or C18. If the sample solutes of interest are not adequately separated on these columns, the CN and Phenyl columns may offer significant differences in selectivity from the straight-chain alkane phases to effect the separation. When using acidic mobile phases (pH <4), the increased bonded-phase stability resulting from StableBond technology means that method ruggedness will be maintained for all of these bonded ligands. In general, larger solutes, such as proteins, are best separated on short-chain reversedphase columns (C3, CN) and peptides and small molecules are separated on longer-chain columns (C8, C18). There are many cases, however, where this conventional wisdom does not apply. For example, peptides can also be effectively separated using short-chain columns, and hydrophobic peptides can show better recovery on longer-chain phases. Therefore, it is best to initially select a phase in the middle of the hydrophobic spectrum (e.g., C8), then change to a more hydrophobic phase or more hydrophilic phase depending on initial results and solubility properties of your sample. 5. pH and Mobile Phase. The choice of mobile phase for a reversed-phase system starts with selecting the type of organic modifier. Selectivity differences and sample retention will vary significantly among mobile phases containing acetonitrile, methanol and tetrahydrofuran (THF). Sample solubility is likely to differ in such solvents and dictate use of a specific solvent or solvents. UV detection at certain wavelengths is not possible with certain modifiers (e.g., methanol at 200 nm). Both pH and ionic strength of the aqueous portion of mobile phases are important parameters in developing rugged methods that are not sensitive to small variations in conditions. With ionic compounds, retention of typical species shows significant changes with pH. It is very important to control pH in such reversed-phase systems to stabilize retention and band spacing. A pH set between 2-4 generally provides the most stable conditions for retention vs. small changes in pH. Stronger acids are an exception to this guideline.
LC and LC/MS
503

Column Selection/Method Development
Column Choice Relative to Application Objective
Application Very high sensitivity, limited sample, LC/MS* High sensitivity, limited sample, LC/MS* Save solvent; special low-volume instrumentation is available Special detectors, e.g. mass spec High sensitivity, limited sample Save solvent; standard HPLC equipment available Standard separations Small-scale (mg) preparative separations Large-scale preparative separations (hundreds of mg-gm) Objective Column Diameter (mm) 0.3, 0.5 1.0 2.1 2.1 2.1 3.0 4.6 9.4 21.2
Consult the Column Hardware section for guard column configurations. *Excellent for peptides and proteins
Method Development from pH 1-12

Chromatographic resolution between two or more peaks depends upon three factors column efficiency, selectivity and retention. With ionizable analytesbases and acidsall of these factors change dramatically with pH. For example, retention can be improved by changing the separation pH, so that analytes are separated in their non-ionized form. Changes in mobile phase pH also improve column efficiency because the ionization of the analyte and the residual silanols can both be altered. This minimizes secondary interactions between analytes and the silica surface that cause poor peak shape. Achieving optimum resolution requires changing the mobile phase pH. The following method development strategy explains how this is done with superior column lifetime. Low, mid and high pH are the three general regions for chromatographic separations as defined in Figure 1. This figure highlights the benefits of performing separations of ionizable analytes in each pH region. Method development proceeds by investigating chromatographic separations first at low pH and then at higher pH until optimum results are achieved. The ideal column is available for each pH region.
LC and LC/MS
504

Method Development
FIGURE 1 Three pH Regions for HPLC Separations of Basic Compounds
Retention Time
B pKa
This figure represents the retention behavior of one basic analyte with respect to pKa and pH. Analyte pKa is 6.5.
10
11
12pH
Analyte charge: Silica surface charge: Ion Exchange Secondary Interaction:
+ neutral NO
+ / neutral neutral / YES -
neutral
NO
Recommended Bonded Phase:
StableBond
Eclipse XDB or Bonus-RP
Extend-C18
Low pH 1-3 Region A

Start method development at low pH, where silanols on a RP-HPLC column are protonated. This minimizes peak tailing by eliminating silanol/base interactions. At low pH, basic compounds are positively charged and their retention may be reduced. Acidic compounds may be protonated and have increased retention. Retention times are usually stable with small changes in pH, producing a robust method. Volatile mobile phase additives, such as formic acid or trifluoroacetic acid (TFA), are often used at low pH with LC/MS.
Mid pH 3-8 Region B

Develop methods at pHs at least 1 pH unit above or below the pKa to minimize changes in retention with small changes in pH. Some silica surface SiOH groups become SiO- above pH 4 to 5; tailing interactions may be possible. Minimize interactions by selecting an endcapped column, using additives such as TEA (less desirable) or using polar-linked bonded phases. Silica breakdown is prevented by innovative bonding chemistry, heavy endcapping and use of Rx-SIL.
High pH 8-12 Region C

In this region, basic compounds may be in their free base form. Increased retention and resolution of basic compounds are likely. Retention changes little in this region-thus robust methods can be developed. Silica breakdown is prevented by innovative bidentate column chemistry, heavy endcapping, use of Rx-SIL and optimum mobile phase. Ammonium hydroxide is an excellent volatile mobile phase modifier at high pH.
LC and LC/MS
505

Method Development Method Development at Low pH is Preferred StableBond Columns
StableBondpH 3
With so many choices available, how do you select a column for method development? This is actually an easy decision. Most analytes today are acidic or basic - ionizable - and the best initial approach is to use a low pH mobile phase (Figure 1, Region A). Using a low pH mobile phase results in the best peak shape for basic compounds because ion-exchange interactions (BH+ + SiOH secondary interaction) are eliminated. Many acidic compounds are noncharged, maximizing their retention at low pH. Therefore, StableBond columns are a good first choice providing the longest lifetime and most robust separations at low pH. For standard analytical work, start method development with acetonitrile as the mobile phase organic modifier and 25-50 mm phosphate buffer (pH 3) as the aqueous component. This provides good pH control, necessary for the most reproducible analyses of ionizable compounds. StableBond SB-C8 or SB-C18 bonded-phases are usually chosen first because they effectively separate most compounds. Changes to the mobile phase (% organic, pH, ionic strength and organic modifier) and column temperature can be used to optimize the separation as necessary. A unique feature of StableBond columns is their high temperature stability. Column temperature can be used as an additional tool to improve resolution.
StableBond Bonded-Phase Selectivity

The other StableBond phasesSB-CN, SB-C3, SB-Phenyl and SB-Aqcan be selected to improve sample resolution. All of these bonded phases are more polar than SB-C8 and SBC18 and can change selectivity. Analysis time for non-polar compounds may also be reduced substantially while retention is maintained for polar compounds. In addition, the SB-CN bonded phase is an excellent choice when you have a mixture of polar and non-polar compounds. It will reduce the retention of the non-polar compounds and possibly eliminate the need for a gradient separation. All of these short-chain bonded phases provide the same exceptional stability as the SB-C8 and SB-C18 columns.
LC and LC/MS
Method Development at Mid pHEclipse XDB and Bonus-RP Columns

Eclipse XDBpH 7
If low pH is not desirable or acceptable for your separation due to poor analyte retention, solubility, selectivity, or stability, then a mid-range pH, such as pH 7, should be investigated (Figure 1, Region B). This usually maximizes differences in retention and selectivity in comparison to low pH. The Eclipse XDB-C18 and C8 bonded-phases are normally the first choice for
506

Method Development
Eclipse XDBpH 7 (continued)
use at this pH because they provide high resolution and superior column lifetime. Phosphate buffer is usually the first choice for mobile phase modifier at pH 7 because its buffer range is pH 6.2-8.2. Phosphate buffers, although commonly used, should be used at temperatures lower than 40C for maximum column life. A second choice for mid pH is acetate buffer since it buffers from pH 3.8-5.8 and is normally selected for LC/MS compatibility. Method development optimization can continue from here by changing the mobile phase, pH, and column temperature (up to 60C) or by changing the bonded phase to Eclipse XDB-Phenyl.
Bonus-RPpH 7
For even more dramatic changes in selectivity or to improve the peak symmetry of basic compounds in the mid-pH range, try the Bonus-RP column. The Bonus-RP column exhibits a modified reversed-phase behavior. When you choose this column, the method development process will be the same as with the Eclipse XDB columns. You can select the same types of buffered mobile phases and use the same organic modifiers; you can even vary the temperature up to 60C. In general, the Bonus-RP is slightly less retentive than alkyl C8 columns because of the polar amine group embedded in the bonded phase. The Bonus-RP column has enhanced stability down to pH 2, so it can also be selected to improve peak shape of difficult basic compounds that tail badly on many other alkyl type phases. Bonus-RP columns can be used with 100% aqueous mobile phases.
Method Development at High pH

Extend-C18 Columns
At low or mid pH, some separations of basic compounds may still not have enough retention or the desired selectivity. For these samples high pH separations may be appropriate (Figure 1, Region C). At high pH, it may be possible to separate bases in their free base form where they are not charged. Retention increases and the chance of obtaining the desired selectivity improves. The Extend-C18 column performs well with high pH mobile phasesup to pH 11.5. For many basic compounds with a pKa of 9-10, this extended pH range allows the separation to take place at least 1 pH unit above the analyte pKa, where it will be in free base form. Method development using the Extend-C18 column is the same as on other columns choose a buffered mobile phase appropriate for the pH range needed and an organic modifier. However, when using the Extend-C18 column organic buffers such as triethylamine, ammonium hydroxide and pyrrolidine are needed for maximum column lifetime; phosphate buffer is not recommended. Methanol is the preferred organic modifier.
LC and LC/MS
507

Method Development ZORBAX Columns Provide Resolution that Lasts
ZORBAX columns have been carefully designed for optimum sample resolution and longest column lifetimes for the low, mid, and high pH ranges. Following the strategy described below simplifies method development using ZORBAX columns. You never have to take a column and make it workyour sample and mobile phases define the best columnso you can always achieve Resolution that Lasts.
A Method Development Strategy that Optimizes Resolution and Column Life

ST ART Basic compounds having low-pH instability SAMPLE Initial separation STEP 1a Add 20 mm TEA or TEA acetate Re-adjust pH Tailing Peaks ZORBAX SB-C18 or SB-C8 pH 2 (1-3) 20-50 mM buffer T = 30C (ambient to 80C, SB-C18 to 90C) Adjust % ACN for 0.5 < k < 20 Band spacing problems STEP 2a Vary column temperature, up to 80C; up to 90C for ZORBAX SB-C18 STEP 2 Band spacing problems Increase or decrease % of organic modifier by 5% (v/v) Band spacing problems STEP 3 Change organic modifier (MeOH or THF) Adjust % organic for 0.5 < k < 20 Restart at STEP [2] Band spacing problems STEP 4 Change bonded-phase functionality to ZORBAX SB-CN, SB-C3 or SB-Phenyl Restart at STEP [1] Band spacing problems STEP 5 OR ZORBAX Eclipse XDB-C8 or XDB-C18 pH 7 (6-9), 20-50 mM buffer T = 30C (ambient to 40C) Adjust % MeOH for 0.5 < k < 20 Band spacing problems STEP 6a Vary temperature within recommended range for bonded phase Band spacing problems Increase or decrease % of organic modifier by 5% (v/v) Band spacing problems Band spacing problems STEP 5d Increase or decrease % of organic modifier by 5% (v/v) STEP 8 Change bonded-phase functionality to ZORBAX XDB-Phenyl or Bonus-RP Restart at STEP [5] Band spacing problems STEP 9 ZORBAX Extend-C18 pH 10.5 (9-12); 5 mM ammonia, or TEA, or 10-50 mM organic buffer, or borate buffer T = 25C (ambient to 40C) Adjust % MeOH for 0.5 < k < 20 Band spacing problems STEP 10a Vary temperature within recommended range for bonded phase Band spacing problems Band spacing problems STEP 10 Change organic modifier (ACN or THF) Adjust for 0.5 < k < 20 Band spacing problems Band spacing problems STEP 5e Change organic modifier (ACN or THF) Adjust for 0.5 < k < 20 Band spacing problems STEP 6 Poor retention or Band spacing problems Poorly retained acidic compounds STEP 5b ACIDIC COMPOUNDS (Ion Pairing) Use MeOH organic modifier, 10-50 mM tetrabutylammonium phosphate, 25 mM pH 7 buffer Adjust % MeOH for 0.5 < k < 20 Band spacing problems STEP 5c Vary temperature within recommended range for bonded phase Band spacing problems STEP 1e Change organic modifier (ACN or THF) Adjust for 0.5 < k < 20 Band spacing problems STEP 1d Increase or decrease % of organic modifier by 5% (v/v) Band spacing problems STEP 1
ZORBAX StableBond Low pH 3
Poorly retained basic compounds
OR
Region A
STEP 1b BASIC COMPOUNDS (Ion Pairing) Use MeOH organic modifier, 10-50 mM hexane sulfonic acid, 25 mM pH 3 buffer Adjust % MeOH for 0.5 < k < 20 Band spacing problems STEP 1c Vary temperature within recommended range for bonded phase
LC and LC/MS
ZORBAX Eclipse XDB ZORBAX Bonus-RP Mid pH 7
Region B
STEP 7 Change organic modifier (ACN or THF) Adjust for 0.5 < k < 20 Restart at STEP [6] Band spacing problems
ZORBAX Extend-C18 High pH 9
Region C
Try different HPLC mode.
Call Agilent Technologies or Your Authorized Agilent Distributor

Adapted and updated by R.D. Ricker, B.A. Bidlingmeyer and J.J. Kirkland from J.J. Kirkland, LC/GC, 14 (1996) 486
508

USP Designations
USP Designations
The US Pharmacopeia (USP) is a standard source for many pharmaceutical methods. The USP specifies columns by packing materials rather than by manufacturer. Listed below are the recommended Agilent Technologies HPLC columns suitable for most LC methods listed with the USP.
USP Designations
USP Packing Materials Columns Particle Size (m) Pore Size ()
L1 Octadecyl silane chemically bonded to porous silica or ceramic micro-particles, 3 to 10 m in diameter
ZORBAX ODS ZORBAX ODS classic ZORBAX Eclipse XDB-C18 ZORBAX Rx-C18, SB-C18 ZORBAX Extend-C18 Hypersil ODS, BDS-C18 LiChrosorb RP-18, LiChrospher RP-18 Purospher RP-18/-e Superspher RP-18/-e Nucleosil 100-5 C18 Vydac 218TP SynChropak C18 ZORBAX SIL ZORBAX Rx-SIL Hypersil LiChrospher 60 Si ZORBAX C8 ZORBAX Eclipse XDB-C8 ZORBAX Rx-C8, SB-C8 Hypersil MOS, BDS-C8 LiChrosorb RP-8 LiChrospher RP-8 LiChrospher RP select B ZORBAX NH2 Hypersil APS LiChrospher NH2
5 5 3.5 and 5 3.5 and 5 3.5 and 5 3 and 5 5, 10 5 5 4 5 5 6.5 5 3.5 and 5 5 5 5 3.5 and 5 3.5 and 5 5 5 5 5 5 5 5
70 70 80 80, 300 (SB) 80, 300 120 100 100 100 100 100 300 300 70 80, 300 120 60 70 80 80, 300 120 100 100 100 70 120 100
L3 Porous silica particles, 5 to 10 m in diameter
L7 Octyl silane chemically bonded to totally porous microsilica particles, 5 to 10 m in diameter
LC and LC/MS
L8 An essentially monomolecular layer of aminopropyl-silane chemically bonded to totally porous silica gel support, 10 m in diameter L9 10 m irregular, totally porous silica gel having a chemically bonded, strongly acidic cationexchange coating
ZORBAX SCX SynChropak SCX
5 spherical 6.5
300 300
509

USP Designations
USP Designations (continued)
USP Packing Materials Columns Particle Size (m) Pore Size ()
L10 Nitrile groups chemically bonded to porous silica particles, 3 to 10 m in diameter
ZORBAX CN ZORBAX SB-CN LiChrospher CN
5 3.5 and 5 5 5 3.5 and 5 3.5 and 5 5
70 80, 300 100 70 80 80 70
L11 Phenyl groups chemically bonded ZORBAX Phenyl to porous silica particles, ZORBAX SB-Phenyl 5 to 10 m in diameter ZORBAX Eclipse XDB Phenyl L13 Trimethylsilane chemically bonded to porous silica particles, 3 to 10 m in diameter L14 Silica gel 10 m in diameter with a chemically bonded, strongly basic quaternary ammonium anion-exchange coating L26 Butyl silane chemically bonded to totally porous silica particles, 5 to 10 m in diameter L33 Packing having the capacity to separate proteins by molecular size over a range of 4,000 to 400,000 daltons. It is spherical, silica-based, and processed to provide pH stability L35 A zirconium-stabilized spherical silica packing with a hydrophilic (diol-type) molecular monolayer bonded phase ZORBAX TMS
ZORBAX SAX SynChropak SAX
5 spherical 6.5
70 300
Vydac C4 SynChropak C4 ZORBAX GF-250 ZORBAX GF-450 Shodex KW Series
5 6.5 4 6
300 300 150 300
GF-250
150
LC and LC/MS
510

Cartridge Column Systems
Cartridge HPLC Column Systems

Agilent offers a variety of popular HPLC analytical columns and guard columns in economical, easy-to-use cartridge configurations.
Cartridges
Icon Type of Cartridge Agilent HPLC Cartridge Features Can reverse collets in the end fitting to add guard cartridges Benefits Inexpensive Extends column lifetime Permits rapid column changes Can use 2,3,4 and 4.6 mm cartridges Helps prevent blockage Protects columns from dirty samples Can be connected by capillaries to different standard cartridge columns Seals at high pressures
Cartridges have a unique filter and sieve at each end Standard Guard Cartridge (nonZORBAX) Rapid exchange Suitable for 2.1 and 4.0 mm id cartridges
ZORBAX Guard Cartridge: Stand alone system
High efficiency, stand-alone, low dead volume cartridge Polymeric cartridge designed for leak-tight seals against metal surfaces Reusable fittings
No gaskets required More solvent-resistant than PEEK Adapt for connection to 1/16 in. LC fittings
LC and LC/MS
ZORBAX Rapid For high-throughput LC/MS, Resolution Cartridge LC/MS/MS and combinatorial Column: 3.5 m packings separations Stand alone system Packed with StableBond for low pH use Packed with Eclipse XDB for mid-range pH use Other packings available on request Sold individually or as three-packs ZORBAX Preparative Guard HPLC Hardware Kit: Stand alone system Easy low dead-volume assembly Tubing (polyphenylene sulfone) designed for leak-tight seals against metal surfaces Reusable fittings Seals up to 2000 psi (135 bar, 13.5 MPa) No gaskets required Low bleed Excellent separations without LC/MS contamination from bonded phase degradation
Adapt for connection to 1/16 in. LC fittings Seals at high pressures
Vydac Guard Cartridge Stand alone system
High efficiency, stand alone, low dead volume cartridge
511

Cartridge/Guard Cartridge Systems Compatibility Guide
Icon Column Type Guard Cartridge Holder ID (mm) Phases
Cartridge column cartridge holder P/N 5021-1845
Guard cartridge (integral system) cartridge holder P/N 5021-1845
2.0 3.0 4.0 4.6
Asahipak Hypersil Lichrospher Nucleosil Purospher Superspher Zorbax Hypersil Lichrosorb
Standard fitting
Column guard cartridge (stand-alone)* 2.1 4.6 cartridge holder P/N 79900CH-010
Standard fitting
Column guard cartridge (stand-alone)* 2.1 3.0 cartridge holder 4.6 P/N 820777-901
ZORBAX
Rapid Resolution
No guard cartridge required
2.1 4.6
ZORBAX
LC and LC/MS
cartridge holder P/N 820222-901
Semi prep. column
Semi prep. guard cartridge (stand-alone)* cartridge holder P/N 840140-901
9.4
ZORBAX
Standard Fitting
Guard cartridge cartridge holder P/N 79918PH-100
2.0 4.0
*Stand-alone guard cartridges fit to all cartridge and standard fitting columns available from Agilent. All columns without icons are standard fitting columns.
512

Cartridge Column Systems Agilent Technologies Cartridge Column System
Agilent offers a variety of popular HPLC packing materials in economical, easy to use cartridge configurations.
Look for this icon identifying Agilent cartridge columns in column ordering tables.
Agilent Cartridge Configuration

Agilents flexible cartridge system has been thoroughly tested to ensure that the design and hardware meet Agilents standards of quality. Finger-tight connections allow rapid column changes without removing the capillaries from the end fittings. The same convenient, easy-touse cartridge holder accommodates 2, 3, 4 and 4.6 mm diameter cartridges of varying lengths. The cartridge columns have a unique filter and sieve at each end that help prevent blockage. By reversing the collets in the end fitting, an inexpensive guard cartridge can be added to further extend column lifetime.
Guard Cartridge Installed
Agilent Cartridge System Accessories

Description Cartridge holder for 2, 3, 4 and 4.6 mm id cartridges Replacement filters for 4 and 4.6 mm id cartridges Replacement filters for 2 and 3 mm id cartridges Mounting tool for replacement filters 10/pk 10/pk Unit Part No. 5021-1845 5063-6574 5063-6519 5021-1846
No Guard Cartridge Installed
LC and LC/MS
513

This icon identifies stand-alone guard cartridge systems for analytical columns.
Guard Cartridge System for Standard Fitting Type Columns

Agilents reusable guard cartridge holder provides for rapid exchange of inexpensive guard columns, which are used to protect valuable columns from dirty samples. This stand-alone system is suitable for both 2.1 and 4.0 mm id guard cartridge columns. It can be connected by capillaries to different standard fitting and cartridge columns. To ensure leak-free and minimum dead-volume connections, Agilent recommends using 1/4 in. wrenches for installation. The guard cartridge system is rigidly tested to meet Agilents high-quality specifications, making it a perfect fit with your HPLC instrumentation. The guard cartridge system is not compatible with PEEK fittings.
Stand Alone Guard Cartridge System
Guard Cartridge System Accessories

Description Guard cartridge holder (for 2.1 and 4 mm id) Connecting capillary 0.12 x 35 mm 4.0 mm id frits and caps for guard column 4.0 mm SS inserts 2.1 mm id frits and caps for guard column 2.1 mm SS inserts 6/pk 6/pk 6/pk 6/pk Unit Part No. 79900CH-010 79841-87609 01090-27613 01090-27614 01090-27606 01090-27611
LC and LC/MS
514

Cartridge Column Systems ZORBAX Guard Cartridge
The ZORBAX Guard Cartridge Series has been developed to provide convenient, cost-effective protection for high performance analytical columns. The cartridge components assemble quickly to provide a high-efficiency, low-dead-volume column that seals at high pressures (up to 3000 psi). The polymeric guard cartridge is specifically designed to make leak-tight seals against metal surfaces without requiring gaskets. This polymeric material (polyphenylenesulfone) is also more solvent-resistant than PEEK. The reusable guard column end fittings adapt the guard cartridge column for connection to 1/16 in. LC fittings and provide a stand-alone guard column system for 2.1-4.6 mm id columns.
This icon identifies stand-alone guard cartridges for ZORBAX analytical columns.
ZORBAX Guard Cartridge System Accessories

Description Guard fittings kit including: guard column holder (1), guard end fitting (1), and column connector (1) Guard end fitting Guard cartridge holder Column connector Part No. 820777-901
ZORBAX Guard Cartridge System
820572-001 820565-001 820563-901
LC and LC/MS
515

Rapid Resolution Cartridge Columns are marked with this icon.
Rapid Resolution Cartridge Column System

For fast, clean high throughput LC/MS, LC/MS/MS and combinatorial separations, we recommend the ZORBAX Rapid Resolution Cartridge Columns. These cartridges are packed with ZORBAX StableBond and Eclipse bonded phases that provide excellent separations without contaminations from bonded phase degradation. Cartridge dimensions are 4.6 x 15 mm or 4.6 x 30 mm and narrow bore sizes 2.1 x 15 mm or 2.1 x 30 mm, all with high purity (99.995%) 3.5 m ZORBAX Rx-SIL-based bonded phase particles. Choose the ZORBAX StableBond SB-C18 and SB-C8 columns for maximum lifetime and exceptional performance at low pH (formic acid and TFA). Use Eclipse XDB-C8 and XDBC18 when mid-pH conditions are required. Upon special request additional packing material can be packed.
Rapid Resolution Cartridge System
These economical and easy-to-use cartridge columns are offered individually and as a convenient three-pack.
Rapid Resolution Cartridge System Accessories

Description Guard fittings kit including: cartridge holder 15 mm (1), cartridge holder 30 mm (1), end fitting assemblies (2) Cartridge holder: 15 mm 30 mm Replacement sealing gaskets End fitting assembly 2/pk Unit Part No. 820222-901
820315-015 820330-030 820370-901 820311-001
LC and LC/MS
516

Cartridge Column Systems/HPLC Column Protection ZORBAX Preparative Guard-Column Hardware Kit
The ZORBAX Preparative Guard-Column has been developed to provide convenient, costeffective protection for high-performance lab-scale preparative columns. The cartridge components assemble quickly and easily to provide a high-efficiency, low-dead-volume column that seals at pressures up to 2000 psi (135 bar, 13.5 MPa). The guard-column tubing made from polyphenylene sulfone is specifically engineered to make leak-tight seals against metal surfaces, without requiring gaskets. The reusable guardcolumn end fittings adapt the cartridge guard-column for connection to standard 1/16 in. LC fittings and provide a stand-alone guard-column system. The ZORBAX materials used in preparative cartridges are matched with chemistry chosen for compatibility with a wide range of applications.
This icon identifies preparative guard-columns.
ZORBAX Preparative Guard-Column Hardware

Description Preparative* guard-column hardware kit including: inlet fitting, end fitting, column connector Part No. 840140-901
Preparative Guard System
*The preparative guard-column hardware is available only as a kit.
HPLC Column Protection

HPLC Column Protection: Guard columns and sample cleanup columns are inexpensive, reproducible, and they increase the accuracy of analysis and analytical column lifetime. Precolumns: The Agilent Technologies precolumn is optimized for guard or sample cleanup tasks and consists of a holder that carries an interchangeable cartridge. With the appropriate cartridge, the precolumn can be used as either a guard column or a sample cleanup column. Guard columns: Guard columns provide protection against contamination without affecting column efficiency. Prepacked cartridges are available for each type of Hypersil material (see pages 565567). Special guard columns are also available see specific table for details. Replacement Column Inlet Frits: If HPLC columns are used without a guard column or in-line precolumn filter, replacement of the column inlet frit may become necessary after extended use with unfiltered samples and/or eluents. PEEK-Encapsulated replacement frits are available for ZORBAX columns packed in 2.1 mm, 3.0 mm, 4.6 mm, and 9.4 mm id standard column hardware.
LC and LC/MS
517

HPLC Column Protection/ZORBAX Silica
Sample Cleanup Kits
Description Sample cleanup column kit: Includes 1 cartridge holder, 2 empty cartridges (2.1 x 20 mm), 4 caps, 4 sieves, 1 funnel, 5 g ODS Hypersil (30 m), connecting capillary Precolumn hardware kit: Includes 1 cartridge holder, 2 empty cartridges (2.1 x 20 mm), 4 caps, 4 sieves, 1 funnel, connecting capillary Part No. 79916KT-100
79916KT-102
Sample cleanup cartridge kit: Includes 5 empty cartridges (2.1 x 20 mm), 79916KT-101 10 caps, 10 sieves Connecting capillary (with preswaged fittings) (0.12 x 35 mm) Replacement caps, for use with cartridges (10/pk) Replacement sieves, for use with cartridges (20/pk) 79841-87609 5061-3306 5061-3305
Packing Material for the Sample Cleanup Column

Phase Hypersil Material ODS ODS Particle Size (m) 10 30 Unit (grams) 5 5 Part No. 79903-85021 79903-85031
Replacement Inlet Frits (PEEK Encapsulated) for Standard Hardware HPLC Columns
Description Narrow Bore Solvent Saver Analytical Unit 10/pk 1/pk 10/pk 1/pk Diameter (mm) 2.1 3.0 4.6 9.4 Part No. 280959-904 280959-006 280959-905 280959-001
LC and LC/MS
Semi-Preparative
Agilent ZORBAX Silica

Agilent ZORBAX Silica is an Essential Part of Providing Resolution that Lasts
The ZORBAX Silica Manufacturing Processthe Making of a Rugged, High-Purity Silica
All Agilent ZORBAX columns are built from porous silica microspheres (PSM) based on silica sols. The silica particle is made of tiny, solid sol microparticles agglutinated in a patented polymerization process, then fused together at very high temperatures to form the final particle (Figure 1). These strong, durable silica particles are called ZORBAX Rx-SIL or ZORBAX SIL and are the base silicas for ZORBAX columns.
518

ZORBAX Silica
The ZORBAX Silica Manufacturing Processthe Making of a Rugged, High-Purity Silica (continued)
The ZORBAX Rx-SIL process produces ultra-pure (99.995%) particles, with very low metal content. The final silica particle is fully hydroxylated and of low acidity. The Rx-SIL process also allows careful and reproducible control of pore size and particle size. These key features: purity (low acidity), strength, and careful control of pore size as well as particle size are critical to excellent chromatographic results and are the building blocks of superior ZORBAX bonded-phases. The table below compares the process used to make the ZORBAX Rx-SIL particles to a second processthe Xerogel processcommonly used to make silica particles for HPLC columns. To produce silica with the key features that maximizes chromatographic performancepurity, strength, controlled pore size and particle size distribution, plus higher pH resistancethe Agilent ZORBAX process is an excellent choice. Lets look at how these features improve your chromatography.
80 or 300 pore
SilicaSol + Urea (pH~2) + CH 2O
O2
3.5, 5, 7 m
StableBond SB, 300SB Eclipse XDB Bonus-RP Extend-C18, 300Extend-C18
FIGURE 1 Formation of ZORBAX Porous Silica Particles
LC and LC/MS
Characteristics of ZORBAX Rx-SIL and a Contrasting Type of Silica

Structure Purity Strength Pore Size, Particle Size Distribution Pore Size/Surface Area Porosity (%) High pH Resistance ZORBAX Rx-SIL (Sol-type) High (99.995%) High Narrow 80/180m2/g 60 Good Xerogel (SIL-type) Low to High Moderate Broad 100/300m2/g 70 Poor
ZORBAX Rx-SIL Uniform Sub Particles Xerogel Sponge-like, Polymeric Network
519

ZORBAX Silica
Original ZORBAX SIL (1973)
CH 2-OH
The Benefit of Silica PurityReduced Peak Tailing

Peak tailing of basic compounds can be a major chromatographic problem. Peak tailing reduces chromatographic efficiency and the accuracy and precision of results. The major cause of peak tailing is interactions between analytes and the silica surface (Figure 2). Typically the presence of acidic silanol sites on the silica surface cause this type of peak tailing. Trace metals in silica increase silanol acidity and peak asymmetry. These silanol interactions are reduced or eliminated by choosing a less acidic, ultrapure (99.995%) silica, such as ZORBAX Rx-SIL. The improvement in chromatography is dramatic. Figure 3 shows the reduction in peak tailing for a basic analyte using ZORBAX Rx-SIL versus a more acidic silica.
NH 2 CH 2CH 3
Highly Purified ZORBAX Rx-SIL

NH 2 CH 2CH 3 CH 2-OH
Silica Particle
SiO - Na + + R3NH +
Silica Particle
SiO - NH +R 3 + Na +
.
1. Ionized silanols (SiO- ) will ion-exchange with protonated bases (R 3NH +) which can cause tailing and method variability
Silica Particle -SiOH + RCOO -
Silica + Particle -SiO - ...H ... - OOCR
2. Unprotonated acids can compete for H + with protonated silanols.
FIGURE 2 Potential Secondary Interactions with Silica Silanols and Ionizable Compounds
The Benefits of Strong ParticlesGreater Efficiency and Durability

Mobile Phase: Flow Rate: 5% Propanol in Heptane 2.0 mL/min
LC and LC/MS
FIGURE 3 Chromatographic Improvement Using Highly Purified ZORBAX Rx-SIL
During the silica manufacturing process, the agglutinated sol particles are sintered for increased strength. This improved mechanical stability allows ZORBAX columns to be packed at high pressures up to 8000 psi! This results in a packed column with an exceptionally stable column bed that will not compress under normal or even high operating pressures up to 5000 psi. This packed bed stability increases column lifetime using ZORBAX 3.5, 5 or 7 m particles. When ZORBAX Rapid Resolution, 3.5 m silica particles are used as the underlying support, high speed, high efficiency chromatography is possible without compromising column lifetime.
520

ZORBAX Silica
The Benefits of Careful Pore Size and Particle Size Control High Efficiency and Better Reproducibility with More Column Choices
Accurate and closely monitored particle and pore size control for ZORBAX Rx-SIL produces reproducible retention behavior from column-to-column and lot-to-lot. The narrow, consistent particle size distribution of ZORBAX Rx-SIL particles maximizes efficiency and column bed stability. Column pressure is never unusually high due to fines smaller particles at the low end of the particle size distribution. Accurate and precise control of particle size allows specific 3.5, 5, and 7 m ZORBAX Rx-SIL particles to be produced. The small 3.5 m particle size is the basis for the Rapid Resolution, high-speed analysis columns designed to maximize resolution in shorter column lengths ideal for LC/MS or any application demanding shorter analysis times. The 5 m particles are an industry standard and provide high resolution in a wide variety of column dimensions. This particle size also provides high efficiency in a short preparative configuration the CombiHT column because careful particle size control means consistent pressure expectations within normal operating limits. The 7 m particle provides the ideal balance between efficiency and operating pressure for longer preparative columns.
ZORBAX Rx-SIL The Foundation for Many Bonded-Phases

With such strong performance characteristics, ZORBAX Rx-SIL particles have been developed into many effective bonded phases for solving key analytical problems. These include columns that can be used at extremes of pH, unmatched by any other silica-based columns. Because silica-based columns have different limitations at low and high pH, specific bonded-phase chemistries are required to provide longer column life over different pH ranges. As a result, Agilent ZORBAX RP-HPLC bonded phases are designed to give extended column lifetime and reproducibility in the pH ranges that provide optimum and long lasting resolution, all starting with high performance ZORBAX Rx-SIL.
LC and LC/MS
521

ZORBAX Silica/ZORBAX Reversed Phase
ZORBAX Reversed-Phase Bonding Summary
The following table summarizes the unique bonding chemistry of ZORBAX RP-HPLC columns. Each is designed for long column lifetime and resolution that lasts in their optimal pH range.
ZORBAX RP-HPLC Column Chemistry

Modern ZORBAX Columns* Extend-C18 Bonus-RP Eclipse StableBond StableBond Aq ZORBAX Rx-C18 Silica Type B B B B B B Side Group Structure on Silane Unique bidentate structure Diisopropyl Dimethyl Diisopropyl (C8, C3, CN, phenyl), diisobutyl (C18) Diisopropyl Dimethyl Polar Group None Amide None None Proprietary None
End-capping Double Triple Double None None None
Original ZORBAX Columns**
ZORBAX ZORBAX ODS Classic
A A
Single None
Dimethyl Dimethyl
None None
*Type B silica: low acidity, low metal content: these bonded phases use ZORBAX Rx -SIL **Type A: more acidic, higher metal content: these bonded phases use ZORBAX SIL
LC and LC/MS
ZORBAX RP-HPLC Bonded Phases

Long Lifetime, Excellent Peak Shape, Superior Resolution from pH 1-12
Agilent ZORBAX reversed-phase bonded phases, based on Rx-SIL, provide the best peak shape and sample resolution, as well as the longest column lifetimes for the low, mid and high pH regions. The retention of ionizable compounds changes with pH, therefore achieving optimum resolution for complex samples requires establishing methods at any pH from pH 1-12. Several types of bonded phases are required to cover this wide pH range and provide maximum column lifetime. The ZORBAX columns described below use unique bonding chemistry to increase the lifetime of the bonded phase and the silica support where needed, thereby providing Resolution that Lasts at every pH.
pH Range for Optimal Column Life 4 5 6 7 8 9 10 11
Recommended Column ZORBAX StableBond ZORBAX Eclipse XDB ZORBAX Bonus-RP ZORBAX Extend-C18
12
13
Unique Selectivity
522

ZORBAX Reversed Phase
For longest lifetime using low pH mobile phase Agilent ZORBAX StableBond
StableBond columns are made using bulky, patented, unique silanes that sterically protect the siloxane bond. Acid labile endcapping reagents are not used. The result is vastly improved column life and extraordinary chemical and temperature stability in the pH 1-6 range for a wide variety of phases (SB-C3, SB-CN, SB-Phenyl, SB-Aq, SB-C8, and SB-C18). Excellent peak shape is expected for many basic compounds because of the ultra-pure Rx-SIL support.
Recommended Column ZORBAX StableBond 1 2 3 pH Range for Optimal Column Life 4 5 6 7 8 9 10 11 12 13
For longest lifetime using mid-range pH mobile phase Agilent ZORBAX Eclipse XDB or Bonus-RP
Eclipse XDB columns are best used in the pH range of 3-8 but can be used successfully over the extended pH range of 2-9. These columns provide excellent peak shape and high resolution for acidic, basic and neutral compounds. The densely-bonded, double-endcapped silica surface minimizes any silanol interactions and protects the silica support from dissolution. Bonus-RP columns provide unique selectivity and applicability for mid pHs with an embedded amide linkage in a C14-alkyl chain. At pH 7, Bonus-RP provides excellent peak shape for even the most challenging basic compounds. Bonus-RP is protected from hydrolysis at low pH using the same sterically protecting bulky side chains as StableBond and is also triple endcapped to minimize silanol interactions and improve stability at mid pH. Bonus-RP can be used without phase collapse in 100% aqueous mobile phases.
Recommended Column ZORBAX Eclipse XDB 1 2 3 pH Range for Optimal Column Life 4 5 6 7 8 9 10 11 12 13
Recommended Column ZORBAX Bonus-RP
12
13
Unique Selectivity
For longest lifetime using high pH mobile phase Agilent ZORBAX Extend-C18
Extend-C18 columns are the newest addition to our product portfolio. These columns incorporate a unique, patented bidentate silane, combined with a double-endcapping process that protects the silica from dissolution at high pHup to pH 11.5. Extend-C18 columns are best applied for separations of compounds that are either (1) basic and have little or no retention at low or intermediate pHs, (2) more stable or more soluble at high pH, or (3) basic and show poor peak shape at low or intermediate pH.
Recommended Column ZORBAX Extend-C18 1 2 3 pH Range for Optimal Column Life 4 5 6 7 8 9 10 11 12 13
LC and LC/MS
523

ZORBAX Reversed Phase
ZORBAX RP-HPLC Columns
Modern ZORBAX RP-HPLC Columns Recommended Uses and Applications
StableBond (SB)
Basic, acidic, neutral compounds Exceptional stability at low pH Use of high temperature (up to 90C for C18, 80C for C8, C3, Phenyl, CN and Aq) and low pH as an added selectivity tool Widest selection of bonded phases for different selectivity (C18, C8, C3, CN, Phenyl, Aq) Uses mobile phases for LC/MS with formic acid, acetic acid, or TFA Uses mobile phases with TFA for peptide and protein separation Rapid separations using 3.5 m columns General separation of basic, acidic, neutral compounds at low pH with different selectivity than SB columns Rx-C8 is the same as SB-C8 Basic, acidic, neutral compounds at mid-range to lower pH Enhanced stability Good separation for lower pH separations (less than 40C) Better peak shape at mid-range pH Rapid separations using 3.5 m columns Separation of dsDNA for restriction digests, PCR fragments, RAPD, RFLP Separating basic compounds in higher aqueous mobile phases General separation of basic, neutral, acidic compounds at mid-range pH or low pH; especially stable at low pH Separating peptides for different selectivity Rapid separations using 3.5 m columns Separating basic compounds above their pKa in free base form; separation of basic, acidic, neutral compounds at high pH; up to pH 11.5 Uses ammonium hydroxide as mobile phase with LC/MS with small molecules or peptides Separating at high, mid-range and low pH for selectivity changes Rapid separations using 3.5 m columns
ZORBAX Rx
Eclipse XDB
Eclipse dsDNA Bonus-RP
Extend-C18
LC and LC/MS
Original ZORBAX Columns
ZORBAX
General separation of basic, acidic, neutral compounds at low pH with different selectivity than SB columns; higher number of active silanols than SB Mixed mode separation at more neutral pH values
ZORBAX ODS Classic General separation of basic, acidic, neutral compounds at mid-range to (non-end capped) low pH with different selectivity than SB or XDB columns
524

80 ZORBAX StableBond
ZORBAX StableBond HPLC Columns for Analytical Chemistry

Longest column lifetime and best reproductibility for low pH separationsdown to pH 1! Patented stable column chemistry allows use at high temperature and low pH without degradation Six different bonded-phases provide broad selectivitySB-C18, SB-C8, SB-CN, SB-Phenyl, SB-C3, SB-Aq High purity silica for superior peak shape Excellent first choice for method development
R O Si R OH R O Si R OH R O Si R R1 R1 R1
Sterically Protected StableBond Bonded Phase

Agilent ZORBAX StableBond columns use patented, unique monofunctional silanes with bulky diisobutyl (SB-C18) or diisopropyl (SB-C8, SB-C3, SB-Phenyl, SB-CN and SB-Aq) side chain groups that sterically protect the key siloxane bond from hydrolytic attack at low pH. StableBond packing materials are not endcapped in order to provide exceptional stability and to maximize lifetime and reproducibility under acidic mobile phase conditions. The high purity, low acidity silica provides excellent peak shape with acidic, basic and neutral compounds so that StableBond columns are an excellent choice for low pH method development. ZORBAX StableBond columns are compatible with all common mobile phases, including very high aqueous mobile phases.
StableBond SB-C18 Shows Excellent Stability at Low pH and High Temperature (pH 0.8, 90C)
1.0 0.8 Relative Retention 0.6 Competitor B - C18 0.4 0.2 0.0 0 5,000 10,000 15,000 20,000 25,000 30,000 Column Volumes of Mobile Phase Purge No column bleed
Bulky diisobutyl (C18) or diisopropyl (C8, C3, CN, Aq, Phenyl) side-chain groups are used to stabilize both long and short chain bonded phases. R=Isopropyl, isobutyl (C18) R1=C8, C18, CN, C3, etc
LC and LC/MS
StableBond SB-C18 (Diisobutyl-C18) ZORBAX Rx-C18 (Dimethyl-C18) Competitor A - C18
Purge Solvent: 50% methanol/50% water with 1.0% TFA Temperature: 90C Solute: Toluene
Competitor C - C18 Competitor D - C18 Competitor E - C18
As an indicator of column breakdown, retention time of toluene was measured after purging the column with mobile phase. Only the StableBond SB-C18 is unchanged after 3 working months of use under these very low pH (0.8) and high temperature (90C) conditions (10,000 column volumes is approximately 1 working month). ZORBAX Rx-C18 also provides a stable matrix and can be used as an alternative selectivity to StableBond SB-C18.
525

Shorter Chain ZORBAX SB-CN is Also Stable at Low pH (pH 2.0, 50C)
ZORBAX StableBond SB-CN, and the other short chain StableBond bonded phases, are also exceptionally stable at low pH. Conventional dimethyl CN and similar bonded phases lack this stability.
100
SB-CN Sterically Protected Silane
80 % k Remaining
60
40 Si-(Me)2PrCN Traditional Silane
20
0 0 1 3 5 4 2 1000 2000 3000 4000 Column Volumes 5000 6000
Five Different Bonded Phases Provide Selectivity Options

SB-C3 is just one of the five different StableBond selectivity choices. In this example, optimum resolution is obtained with SB-C3. All are based on the same high purity Rx-SIL. Selectivity changes are therefore dependent on the bonded phases making method development more reliable. ZORBAX StableBond 4.6 x 150 mm, 5 m Mobile Phase: 0-100% B in 18.8 min A: 50 mm NaH2PO4, pH 2.5 in 95% H2O / 5% ACN B: 50 mm NaH2PO4, pH 2.5 in 47% H2O / 53% ACN Flow rate: 1.0 mL/min Temperature: 26C UV Detection: 254 nm Sample: 1. Procaine 2. Lidocaine 3. d-Cinchonine 4. Butacaine 5. Tetracaine Columns:
StableBond SB-C18 (P/N 883975-902)

3 1 5 4 2
LC and LC/MS
3 5 4 2

3 5 4 2
StableBond SB-Phenyl (P/N 883975-912)

1 3 4 2 5
5 Time (min)
10
15
StableBond SB-CN (P/N 883975-905)
526

80 ZORBAX StableBond Excellent Peak Shape
The high purity Rx-SIL used in the manufacturing of all StableBond columns provides excellent peak shape of basic and acidic compounds. ZORBAX SB-C18 4.6 x 150 mm, 5 m (P/N 883975-902) Mobile Phase: 85% 0.1% TFA: 15% ACN Flow rate: 1.0 mL/min Temperature: 35C UV Detection: 210 nm Sample: 5 L 1. Aspartic Acid 2. Phenylalanine 3. 5-benzyl-3, 6-dioxo-2-piperazineacetic acid 4. Asp-phe 5. Aspartame Columns:
2 3
10 Time (min)
15
20
SB-CN Optimizes Retention and Resolution

The SB-CN column is used here to reduce analysis time by 50%. The retention of the most hydrophobic analyte is cut in half. At the same time, retention of the more polar, early eluting peaks increases slightly. Columns: ZORBAX SB 4.6 x 75 mm, 3.5 m Mobile Phase: 30% ACN, 70% NaH2PO4 pH 2.5 Flow rate: 1.0 mL/min Temperature: 35C Sample: 1. Estriol 2. Daidzen 3. Quercetin 4. Genistein 5. Diethylstilbestrol
1
4 3 5
10
15 Time (min)
20
25
LC and LC/MS
SB-C18 (P/N 866953-902)

5
2 4
3 1
6 8 Time (min)
10
12
SB-CN (P/N 866953-905)
527

80 ZORBAX StableBond ZORBAX Columns for Analytical ChemistryStableBond
ZORBAX StableBond Columns (80)
Icon Column Description Standard Columns Size (mm) Particle Size (m) SB-C18 USP L1 SB-C8 USP L7 SB-CN USP L10 SB-C3 SB-Phenyl USP L11 SB-Aq
Preparative Semi-Preparative Semi-Preparative Semi-Preparative Semi-Preparative Analytical Analytical Analytical Rapid Resolution Rapid Resolution Rapid Resolution Rapid Resolution Solvent Saver Solvent Saver Solvent Saver Plus Solvent Saver Plus Narrow Bore Narrow Bore Narrow Bore RR* Narrow Bore RR Narrow Bore RR Narrow Bore RR MicroBore RR MicroBore RR MicroBore RR MicroBore Guard
21.2 x 250 9.4 x 250 9.4 x 150 9.4 x 100 9.4 x 50 4.6 x 250 4.6 x 150 4.6 x 50 4.6 x 150 4.6 x 100 4.6 x 75 4.6 x 50 3.0 x 250 3.0 x 150 3.0 x 150 3.0 x 100 2.1 x 150 2.1 x 50 2.1 x 150 2.1 x 100 2.1 x 75 2.1 x 50 1.0 x 150 1.0 x 50 1.0 x 30 1.0 x 13.5
7 5 5 5 5 5 5 5 3.5 3.5 3.5 3.5 5 5 3.5 3.5 5 5 3.5 3.5 3.5 3.5 3.5 3.5 3.5 5 7 5 5
880975-102 880967-106 880975-105 883975-202 884975-202 846975-202
880975-112
880975-202 880967-201 880975-205 880975-209 880975-212
880975-902 880975-906 880975-905 880975-909 880975-912 880975-914 883975-902 883975-906 883975-905 883975-909 883975-912 883975-914 846975-902 846975-906 863953-902 863953-906 863953-905 861953-902 861953-906 861953-905 866953-902 866953-906 866953-905 835975-902 835975-906 835975-905 846975-914 863953-912 863953-914 861953-912 861953-914 866953-912 866953-914 835975-912 835975-914
880975-302 880975-306 880975-305 880975-309 880975-312 883975-302 883975-306 883975-305 883975-309 883975-312 863954-302 863954-306 863954-305 861954-306 883700-922 883700-906 883700-905 883700-909 883700-912 860975-902 860975-906 860975-905 860975-909 860975-912 860975-914 830990-902 830990-906 861753-902 861753-906 861753-905 866735-902 871700-902 871700-906 863600-902 863600-906 863600-905 865600-902 865600-906 861600-902 861600-906 5185-5920 5185-5920 820675-115 820675-115 820675-115 820675-124 871700-914 830990-914 861753-912 861753-914 863954-312
LC and LC/MS
Guard cartridge, 2/pk 9.4 x 15 Guard cartridge, 4/pk 4.6 x 12.5 Guard cartridge, 4/pk 2.1 x 12.5 Guard hardware kit Guard hardware kit Guard hardware kit 9.4 x 15 4.6 x 12.5 2.1 x 12.5
820950-920 820950-915 820950-916 820950-922 820950-917 820950-933 821125-926 821125-926 821125-924 821125-924 821125-926 821125-933 840140-901 840140-901 840140-901 840140-901 840140-901 820777-901 820777-901 820777-901 820777-901 820777-901 820777-901 820777-901 820777-901 820777-901 820777-901 820777-901 820777-901
*RR: Rapid resolution 3.5 m columns Configurations not shown are available upon request. For more information on these and other columns consult the Agilent web site at www.agilent.com.
528

ZORBAX StableBond Columns (80)
Icon Column Capillary Glass-lined Column Size (mm) Particle Size SB-C18 (m) USP L1 SB-C8 USP L7
Capillary Capillary Capillary Capillary RR* Capillary RR Capillary Capillary Capillary Capillary RR Replacement Screens, 10/pk
Agilent Cartridge Column
0.5 x 250 0.5 x 150 0.5 x 35 0.5 x 150 0.5 x 35 0.3 x 250 0.3 x 150 0.3 x 35 0.3 x 150
5 5 5 3.5 3.5 5 5 5 3.5
5064-8258 5064-8256 5064-8254 5064-8262 5064-8260 5064-8257 5064-8255 5064-8253 5064-8261 5065-4427
Analytical Analytical Rapid Resolution Guard Cartridge 10/pk Cartridge Holder Rapid Resolution Cartridge Rapid Resolution Cartridge, 3/pk Rapid Resolution Cartridge Rapid Resolution Cartridge, 3/pk Rapid Resolution Cartridge Rapid Resolution Cartridge, 3/pk Rapid Resolution Cartridge Rapid Resolution Cartridge, 3/pk High Throughput Cartridge Column Hardware Kit Replacement Seals, 2/pk CombiHT CombiHT CombiHT CombiHT End Fittings (2) (required for use) Replacement Seal (1 ea)
4.6 x 250 4.6 x 150 4.6 x 75 4.0 x 4.0
5 5 3.5 5
7995218-585 7995208-585 7995218-595 7995208-595 7995218-344 7995208-344 7995118-504 7995118-504 5021-1845 5021-1845 833975-906 833975-936 831975-906 831975-936 873700-906 873700-936 875700-906 875700-936 820222-901 820370-901
High Throughput Cartridge Columns (requires hardware kit P/N 820222-901)
4.6 x 30 4.6 x 30 4.6 x 15 4.6 x 15 2.1 x 30 2.1 x 30 2.1 x 15 2.1 x 15
3.5 3.5 3.5 3.5 3.5 3.5 3.5 3.5
833975-902 833975-932 831975-902 831975-932 873700-902 873700-932 875700-902 875700-932 820222-901 820370-901
LC and LC/MS
CombiHT High Throughput Preparative Columns (endfittings required)
21.2 x 150 5 21.2 x 100 5 21.2 x 50 5
870150-902 870150-906 870100-902 870100-906 870050-902 870050-906 820400-901 820400-901 820385-901 820385-901
*RR: Rapid Resolution 3.5 m columns. Configurations not shown are available upon request. For more information on these and other columns consult the Agilent web site at www.agilent.com.
529

ZORBAX StableBond Column Specifications
Bonded Phase Pore Size Surface Area Temp. Limits* pH Range Endcapped Carbon Load
ZORBAX SB-C18 ZORBAX SB-C8 ZORBAX SB-C3 ZORBAX SB-CN ZORBAX SB-Aq
80 80 80 80 80
180 m2/g 180 180 180 m2/g m2/g m2/g
90C 80C 80C 80C 80C 80C
1.0 - 8.0 1.0 - 8.0 1.0 - 8.0 1.0 - 8.0 1.0 - 8.0 1.0 - 8.0
No No No No No No
10% 5.5% 4.0% 4.0% 5.5% Proprietary
ZORBAX SB-Phenyl 80
180 m2/g 180 m2/g
*StableBond columns are designed for optimal use at low pH. At pH 6-8, highest column stability for all silica-based columns is obtained by operating at temperatures <40C and using low buffer concentrations in the range of 0.01-0.02 M. At mid-range pH, Eclipse XDB and Bonus-RP are recommended.
ZORBAX SB-Aq HPLC Columns

Recommended for high resolution analysis of very polar compounds Achieve high retention in highly aqueous mobile phases Obtain reproducible retention without phase collapse Provide exceptional column lifetime at low pH
LC and LC/MS
ZORBAX SB-Aq columns are an ideal choice to increase the retention of many difficult acidic, basic and polar compounds. More retention may be obtained than on many conventional ODS HPLC columns. Polar compounds can be analyzed with excellent reproducibility and peak shape because SB-Aq columns have a hydrophilic surface that inhibits phase collapse, even with 100% aqueous mobile phases. ZORBAX StableBond technology adds exceptional durability and stability at low pH and high temperature down to pH 1 and up to 80C with the SB-Aq column.
Excellent Retention in a High Aqueous Mobile Phase on the SB-Aq Column

Basic compounds that are very polar and difficult to retain and separate can be analyzed without using a gradient method. The SB-Aq column increases the retention of the early eluting compounds and resolves all components using a simple isocratic, highly aqueous mobile phase.
1
Sample: 1. Maleate 2. Phenylephrine 3. Phenylpropanolamine 4. Pyrilamine 5. Chlorpheniramine

3 4 5
ZORBAX SB-Aq 4.6 x 150 mm, 5 m (P/N 883975-914) Mobile Phase: 90% 0.2% TFA 10% ACN Temperature: 25C Flow Rate: 1.5 mL/min Detection: UV 254 nm
Column:
8 10 Time (min)
12
14
16
530

Separation of Water Soluble Vitamins on the SB-Aq Column without Ion Pairing
The SB-Aq column is used with a simple mobile phase (no ion-pair reagents) to achieve sufficient retention and high resolution of these B vitamins.
1
3 4
0.0
0.5
1.0 Time (min)
1.5
2.0
ZORBAX SB-Aq 4.6 x 150 mm, 5 m (P/N 883975-914) Mobile Phase: 95% 0.1% TFA 5% MeOH Temperature: 35C Flow Rate: 2.0 mL/min Detection: UV 254 nm Sample: 1. Thiamine (B1) 2. Nicotinic Acid 3. Pyridoxine (B6) 4. Niacinamide
Column:
ZORBAX SB-Aq Column Specifications

Bonded Phase Pore Size Surface Area Temp. Limits pH Range Endcapped
ZORBAX SB-Aq
80
180
m2/g
80C
1.0 - 8.0
No
ZORBAX StableBond Aq (SB-Aq) Column

Icon Column Description Dimension (mm) Particle Size (m) Unit Part No.
Analytical Analytical Analytical Rapid Resolution Rapid Resolution Rapid Resolution Rapid Resolution Narrow Bore Narrow Bore RR Narrow Bore RR Narrow Bore RR Guard Cartridges Guard Cartridges Guard Hardware Kit
4.6 x 250 4.6 x 150 4.6 x 50 4.6 x 150 4.6 x 100 4.6 x 75 4.6 x 50 2.1 x 50 2.1 x 150 2.1 x 100 2.1 x 50 4.6 x 12.5 2.1 x 12.5
5 5 5 3.5 3.5 3.5 3.5 5 3.5 3.5 3.5 5 5 4/pk 4/pk
880975-914 883975-914 846975-914 863953-914 861953-914 866953-914 835975-914 860975-914 830990-914 861753-914 871700-914 820950-933 821125-933 820777-901
LC and LC/MS
Configurations not shown are available upon request. For more information on these and other columns consult the Agilent web site at www.agilent.com.
531

ZORBAX Wide-Pore 300StableBond HPLC Columns for BioAnalytical Chemistry

Four different bonded phases for reversed-phase selectivity optimization of peptides and proteins Exceptional stability and long lifetime at low pH with TFA containing mobile phases Excellent reproducibility Stable at high temperatureup to 80-90C
ZORBAX 300StableBond columns are an ideal choice for the reproducible separations of proteins and peptides for two key reasons. First, wide-pore, 300 columns are necessary for an efficient separation of proteins and peptides, or other large molecules, in order to allow these analytes to completely access the bonded phase. Second, 300StableBond columns are unmatched in their durability at low pH, such as with the TFA containing mobile phases typically used for protein and peptide separations. For LC/MS separations at low pH, 300StableBond columns can also be used with formic acid and acetic acid mobile phase modifiers. 300StableBond columns are available in four different bonded phases (C18, C8, C3, and CN) for selectivity and recovery optimization of proteins and polypeptides. To further increase sample recovery and improve efficiency for difficult proteins, 300StableBond columns can be used up to 80-90C.
R O Si R OH R O Si R OH R O Si R R1 R1 R1
Sterically Protected 300StableBond Bonded Phase

Bulky diisobutyl (C18) or diisopropyl (C8, C3, CN) side-chain groups are used to stabilize both long and short chain bonded phases. Additional stability information on StableBond columns can be found on page 525. R=Isopropyl, isobutyl (C18), R1=C8, C18, CN, C3
LC and LC/MS
532

Short-Chain ZORBAX 300SB-C3 is Stable at Low pH, High Temperature
% k Phenylheptane Remaining
100 90 80 70 60 50 40 0 1000 2000 3000 4000 5000 6000 Volume of Mobile Phase (mL) 7000 8000 ZORBAX 300SB-C3 Competitor C4
300StableBond columns are unsurpassed in their low pH stability. The reproducible monolayer bonding and sterically protected bonded phase increase the lifetime and reliability of the short-chain 300SB-C3 column over another wide-pore C4 column. Additional stability information on StableBond columns can be found on page 525. Aging Conditions: Gradients 0-100% B in 80 min. (continuous) A: 0.5% TFA in water B: 0.5% TFA in acetonitrile Isocratic Retention Test Conditions: 1-phenylheptane 50% A, 50% B Columns: 4.6 x 150 mm Temperature: 60C Flow Rate: 1.0 mL/min
Four Different ZORBAX 300SB Bonded Phases Optimize Separation of Large Polypeptides
The 300SB-C18, C8, C3 and CN bonded phases all provide a different separation of this group of polypeptides. This adds an important parameter for quickly optimizing protein separations. The 300SB-CN column offers unique selectivity for more hydrophilic polypeptides. Columns: StableBond 300SB 4.6 x 150 mm, 5 m Mobile Phase: Linear Gradient, 25-70% B in 40 min. A: 0.1% TFA in Water B: 0.09% TFA in 80% Acetonitrile/20% Water Flow Rate: 1.0 mL/min Temperature: 60C Sample: 3 g each protein 1. RNase 2. Insulin 3. Cytochrome C 4. Lysozyme 5. Parvalbumin 6. CDR 7. Myoglobin 8. Carbonic Anhydrase 9. S-100 10. S-100
2,3 1 4 5 6 7 8 9 10
300SB-C18 P/N 883995-902

6,7 4 3 5 8 9 10
LC and LC/MS
300SB-C8 P/N 883995-906
2 3
7 6
8 9 10
ZORBAX StableBond 300SB Column Specifications

Bonded Phase Pore Size Surface Area Temp. Limits* pH Range Endcapped Carbon Load
300SB-C3 P/N 883995-909

1 2 3 7 6
8 9 10
ZORBAX 300SB-C18 ZORBAX 300SB-C8 ZORBAX 300SB-C3 ZORBAX 300SB-CN
300 300 300 300
45 45
m2/g m2/g
90C 80C 80C 80C
1.0 - 8.0 1.0 - 8.0 1.0 - 8.0 1.0 - 8.0
No No No No
2.8% 1.5% 1.1% 1.2%

0 5 10 15 20 25 Time (min)
45 m2/g 45 m2/g
30
35
40
300SB-CN P/N 883995-905
*300StableBond columns are designed for optimal use at low pH. At pH 6-8, highest column stability for all silica-based columns is obtained by operating at temperatures <40C and using low buffer concentrations in the range of 0.01-0.02M. At mid or high pH, 300Extend-C18 is recommended.
533

ZORBAX StableBond (300) Column
Icon Column Description Standard Columns Size (mm) Particle Size (m) 300SB-C18 USP L1 300SB-C8 USP L7 300SB-CN USP L10 300SB-C3
Preparative Semi-Preparative Analytical Analytical Analytical Rapid Resolution Rapid Resolution Rapid Resolution Solvent Saver Plus Solvent Saver Plus Narrow Bore Narrow Bore RR* Narrow Bore RR Narrow Bore RR MicroBore RR MicroBore RR MicroBore Guard Guard cartridges, 2/pk
21.2 x 250 9.4 x 250 4.6 x 250 4.6 x 150 4.6 x 50 4.6 x 150 4.6 x 100 4.6 x 50 3.0 x 150 3.0 x 100 2.1 x 150 2.1 x 150 2.1 x 100 2.1 x 50 1.0 x 150 1.0 x 50 1.0 x 13.5 9.4 x 15 4.6 x 12.5 2.1 x 12.5 9.4 x 15 4.6 x 12.5 2.1 x 12.5 0.5 x 250 0.5 x 150 0.5 x 35 0.5 x 150 0.5 x 35 0.3 x 250 0.3 x 150 0.3 x 35 0.3 x 150 0.3 x 100 0.3 x 75 0.3 x 50
7 5 5 5 5 3.5 3.5 3.5 3.5 3.5 5 3.5 3.5 3.5 3.5 3.5 5 7 5 5
880995-102 880995-202 880995-902 883995-902 860950-902 863973-902 861973-902 865973-902
880995-106 880995-206 880995-906 883995-906 860950-906 863973-906 861973-906 865973-906 863974-306 861973-306
880995-105 880995-205 880995-905 883995-905 860950-905 863973-905 865973-905
880995-109 880995-209 880995-909 883995-909 860950-909 863973-909 865973-909
883750-902 861775-902 865750-902 863630-902 865630-902 5185-5920 820675-124 820950-921 821125-918 840140-901 820777-901 820777-901
883750-906 863750-906 861775-906 865750-906 863630-906 865630-906 5185-5920 820675-124 820950-918 821125-918 840140-901 820777-901 820777-901
883750-905
883750-909
820675-124 820950-923 821125-924 840140-901 820777-901 820777-901
820675-124 820950-924 821125-924 840140-901 820777-901 820777-901
LC and LC/MS
Guard cartridges, 4/pk Guard cartridges, 4/pk Guard hardware kit Guard hardware kit Guard hardware kit Capillary Capillary Capillary Capillary RR Capillary RR Capillary Capillary Capillary Capillary RR Capillary RR Capillary RR Capillary RR Replacement screens, 10/pk
Capillary Glass-lined Columns
5 5 5 3.5 3.5 5 5 5 3.5 3.5 3.5 3.5
5064-8266 5064-8264 5064-8294 5064-8268 5065-4459 5064-8265 5064-8263 5064-8295 5064-8267 5064-8259 5064-8270 5064-8300 5065-4427 5065-4460 5065-4461 5065-4462 5065-4463 5065-4427 www.agilent.com/chem
Configurations not shown are available upon request. *RR: Rapid Resolution 3.5 m columns.
534

Poroshell
Poroshell 300SB-C18 HPLC Columns for Bioanalytical Chemistry

Revolutionary new particle for high velocity, reversed phase separations of proteins High speed and high resolution of proteins and other large molecules Ultra-short analysis times with high flow velocities Outstanding stability at low pH with 300StableBond bonded phase
Porous Shell SOLID CORE 5 m
Solid Core/Poroshell
Poroshell Particles have a Superficially Porous Surface and a Solid Core

The revolutionary new Poroshell 5 m particle combines a solid core with a 300 porous surface layer bonded with SB-C18. The result is a highly efficient particle for rapid, high-resolution separations of proteins and other large molecules.
Fast, High Resolution Separation of Peptides and Proteins with Poroshell 300SB-C18...in Seconds
Column: Poroshell 300SB-C18 2.1 x 75 mm, 5 m P/N 660750-902 Mobile Phase: A. 0.1% TFA B. 0.07% TFA in ACN Gradient: 5-100% B in 1.0 min Flow Rate: 3.0 mL/min Temperature: 70C Pressure: 260 bar Detection: UV 215 nm Sample: 1. Angiotensin II 2. Neurotensin 3. RNase 4. Insulin 5. Lysozyme 6. Myoglobin 7. Carbonic Anhydrase 8. Ovalbumin
3 1 2
7 6 8
0.0
0.5 Time (min)
1.0
LC and LC/MS
Spaces between solutes indicate good peak capacity for rapidly separating complex samples
Very High Resolution at High Flow Velocities on Poroshell 300SB-C18 Column

High flow velocities can be used on the Poroshell 300SB-C18 column to reduce analysis time with the highest column efficiency. At these high flow velocities, sharp and efficient peaks are obtained because of the more rapid mass transfer in comparison to the totally porous particle.
300SB-C18 Totally Porous Particle
0 0.1 0.2 0.3 2 3 1,2 3
0.4 4
0.5
Poroshell 300SB-C18
(Agilent Part No. 660750-902)
Poroshell 300SB-C18 2.1 x 75 mm, 5 m P/N 660750-902 Gradient: 5-100% B in 0.7 min Mobile Phase: A. 95% H2O, 5% ACN with 0.1% TFA B. 5% H2O, 95% ACN with 0.07% TFA Flow Rate: 3.0 mL/min Temperature: 70C Detector: UV 215 nm
Column:
Sample: 1L inj, (200 g/mL each) 1. Neorotensin 2. RNaseA 3. Lysozyme 4. Myoglobin
0.1
0.2 0.3 Time (min)
0.4
0.5
535

Poroshell/ZORBAX Eclipse XDB
Poroshell 300SB-C18 Column
Column Description Standard Column Unit Size (mm) Particle Size (m) Part Number
Narrow Bore
2.1 x 75
660750-902
Cartridge Column* (Requires Hardware Kit, P/N 5185-5919)
Narrow Bore RR Guard cartridge Hardware kit 4/pk
2.1 x 75 2.1 x 12.5
5 5
677700-902 821075-920 820333-901

* Available early in 2002
Note: Check for availability of Poroshell capillary columns in Spring 2002
ZORBAX Eclipse XDB HPLC Columns

Excellent peak shape for bases, acids and neutrals Long lifetime at mid pH with extra dense bonding and double endcapping Extended pH rangepH 2-9
Recommended Column ZORBAX Eclipse XDB 1 2 3 pH Range for Optimal Column Life 4 5 6 7 8 9 10 11 12 13
CH 3 O Si CH 3 CH 3 O Si CH 3 CH 3 O Si CH 3 CH 3 O Si CH 3 CH 3 O Si CH 3 CH 3 CH 3
The Agilent ZORBAX Eclipse XDB columnsC18, C8 and Phenylare designed to provide good peak shape for basic compounds at mid pH. In the mid-pH region residual silanols are more active and tailing interactions are more likely. To overcome these interactions, Eclipse XDB columns are eXtra Densely Bonded and then double end-capped through a proprietary process to cover as many active silanols as possible. Column lifetime at mid pH is enhanced through the dense bonding and the choice of the thick-walled Rx-SIL support, which resists silica dissolution in the mid-pH range. As a result, the Eclipse XDB columns can be used over the pH range 2-9, with an optimum lifetime from pH 3-8. The Eclipse XDB columns also provide an alternate selectivity to the StableBond, Bonus-RP and Extend-C18 columns.
LC and LC/MS
Extra Densely Bonded and Double Endcapped Eclipse XDB Bonded Phase
536

ZORBAX Eclipse XDB
Column Stability Testing
Double endcapping, dense bonding, and the durable Rx-SIL particles (sol-type) combine to provide long lifetime at pH 7.0 when compared to single endcapped sil-gel columns, such as the Waters Symmetry C8 column used here. The conditions used for this testhigh temperature (60C) and high salt concentration (250 mM), accelerate the dissolution of silica, causing premature failure of the sil-gel type column.
0 1 2 1 5
3 4
4 Time (min)
Eclipse XDB-C8 Sol-type After 1843 Column Volumes
Column: 4.6 x 150 mm, 5 m Mobile Phase: 60% ACN 40% 250 mM phosphate buffer, pH 7.0 Flow Rate: 1.5 mL/min Temperature: 60C
Sample: Tricyclic Antidepressants 1. Uracil 2. Nortriptyline 3. Doxepin 4. Amitriptyline 5. Trimipramine

0
5 2,3
10 11 12
Time (min)
Waters Symmetry C8 SIL-type After 1826 Column Volumes
Good Peak Shape for Acids, Bases, and Neutrals on ZORBAX Eclipse XDB at Mid-pH
In the mid-pH region, residual silanols can interact more strongly with analytes, resulting in asymmetric peaks. The Eclipse XDB column provides good peak shape in this difficult pH range.
LC and LC/MS
ZORBAX Rapid Resolution Eclipse XDB-C8 4.6 x 75 mm, 3.5 m P/N 966967-906 Mobile Phase: 35% 20 mM NaH2PO4, pH 7.0, 65% MeOH Flow Rate: 1.0 mL/min Temperature: 35C Detection: UV 254 nm
Column:
Sample: 1. Uracil 2. Butyl Paraben 3. Propranolol 4. Naphthalene 5. Dipropyl Phthalate 6. Acenaphthene 7. Amitriptyline
1 2 3 5
4 6 7
5 Time (min)
10
15
537

ZORBAX Eclipse XDB
2
Selectivity Changes for Basic Compounds with Eclipse XDB and StableBond
Eclipse XDB and StableBond columns are based on the same silica but have different bonding and endcapping. Therefore, they can have very different selectivity for the same sample under the same conditions.
0.0
0.5
1.0
1.5
2.0 2.5 Time (min)
3.0
3.5
4.0
4.5
Non-Endcapped StableBond SB-C8 P/N 866953-906

2
Rapid Resolution 4.6 x 75 mm, 3.5 m Mobile Phase: 70% 25 mM NaH2PO4, pH 3.0, 30% Methanol Flow Rate: 1.0 mL/min Temperature: 35C
Columns:
Sample: 1. Barbital 2. Sulfamethoxazole 3. Caffeine
0.0
0.5
1.0
1.5
2.0
2.5
3.0
3.5
4.0
4.5
Time (min)
Double Endcapped Eclipse XDB-C8 P/N 966967-906
ZORBAX Eclipse XDB Column Specifications

Bonded Phase Pore Size Surface Area Temp. Limits pH Range Endcapped Carbon Load
ZORBAX Eclipse XDB-C18 ZORBAX Eclipse XDB-C8
80 80
180 m2/g 180 180 m2/g m2/g
60C 60C 60C
2.0-9.0 2.0-9.0 2.0-9.0
Double Double Double
10% 7.6% 7.2%
LC and LC/MS
ZORBAX Eclipse XDB-Phenyl 80
538

ZORBAX Eclipse XDB
ZORBAX Eclipse XDB Column
Icon Column Description Standard Columns Unit Size (mm) Particle Size (m) XDB-C18 USP L1 XDB-C8 USP L7 XDB-Phenyl USP L11
Semi-Preparative Analytical Analytical Analytical Rapid Resolution Rapid Resolution Rapid Resolution Rapid Resolution Solvent Saver Solvent Saver Solvent Saver Plus Solvent Saver Plus Narrow Bore Narrow Bore Narrow Bore RR* Narrow Bore RR Narrow Bore RR Narrow Bore RR MicroBore RR MicroBore RR MicroBore RR MicroBore Guard Guard cartridges Guard cartridges Guard hardware kit Guard hardware kit 4/pk 4/pk
9.4 x 250 4.6 x 250 4.6 x 150 4.6 x 50 4.6 x 150 4.6 x 100 4.6 x 75 4.6 x 50 3.0 x 250 3.0 x 150 3.0 x 150 3.0 x 75 2.1 x 150 2.1 x 50 2.1 x 150 2.1 x 100 2.1 x 75 2.1 x 50 1.0 x 150 1.0 x 50 1.0 x 30 1.0 x 13.5 4.6 x 12.5 2.1 x 12.5 4.6 x 12.5 2.1 x 12.5
5 5 5 5 3.5 3.5 3.5 3.5 5 5 3.5 3.5 5 5 3.5 3.5 3.5 3.5 3.5 3.5 3.5 5 5 5
990967-202 990967-902 993967-902 946975-902 963967-902 961967-902 966967-902 935967-902 990967-302 993967-302 963954-302 966954-302 993700-902 960967-902 930990-902 961753-902 966735-902 971700-902 963600-902 965600-902 961600-902 5185-5921 820950-925 821125-926 820777-901 820777-901
990967-206 990967-906 993967-906 946975-906 963967-906 961967-906 966967-906 935967-906 990967-306 993967-306 963954-306 993700-906 960967-906 930990-906 961753-906 971700-906 966967-912 935967-912 990967-312 993967-312 963954-312 993700-912 960967-912 963967-912 990967-912 993967-912
and LC/MS
963600-906 965600-906 961600-906 5185-5921 820950-926 821125-926 820777-901 820777-901 820950-927 821125-926 820777-901 820777-901
*RR: Rapid Resolution 3.5 m columns. Configurations not shown are available upon request.
539

ZORBAX Eclipse XDB
ZORBAX Eclipse XDB Column (continued)
Icon Column Description Unit Size (mm) Particle Size (m) XDB-C18 USP L1 XDB-C8 USP L7
Capillary Glass-lined Columns
Capillary Capillary Capillary Capillary RR* Capillary RR Capillary Capillary Capillary Capillary RR Replacement screens 10/pk
0.5 x 250 0.5 x 150 0.5 x 35 0.5 x 150 0.5 x 35 0.3 x 250 0.3 x 150 0.3 x 35 0.3 x 150
5 5 5 3.5 3.5 5 5 5 3.5
5064-8286 5064-8287 5064-8296 5064-8288 5064-8298 5064-8269 5064-8291 5064-8297 5064-8271 5065-4427
Agilent Cartridge Columns (requires hardware kit P/N 5021-1845)
Analytical Analytical Rapid Resolution Solvent Saver Guard cartridges Cartridge holder 10/pk
4.6 x 250 4.6 x 150 4.6 x 75 3.0 x 75 4.0 x 4
5 5 3.5 3.5 5
7995118-585 7995118-595 7995118-344 7995230-344 7995118-504 5021-1845
7995108-585 7995108-595 7995108-344 7995118-504 5021-1845
High Throughput Cartridge Columns (requires hardware kit P/N 820222-901)
LC and LC/MS
Rapid Resolution Cartridge Rapid Resolution Cartridge Rapid Resolution Cartridge Rapid Resolution Cartridge Rapid Resolution Cartridge Rapid Resolution Cartridge Rapid Resolution Cartridge Rapid Resolution Cartridge Hardware kit for high throughput columns Replacement seals 2/pk 3/pk 3/pk 3/pk 3/pk
4.6 x 30 4.6 x 30 4.6 x 15 4.6 x 15 2.1 x 30 2.1 x 30 2.1 x 15 2.1 x 15
3.5 3.5 3.5 3.5 3.5 3.5 3.5 3.5
933975-902 933975-932 931975-902 931975-932 973700-902 973700-932 975700-902 975700-932 820222-901 820370-901
933975-906 933975-936 931975-906 931975-936 973700-906 973700-936 975700-906 975700-936 820222-901 820370-901
CombiHT Columns (end fittings required)
CombiHT CombiHT CombiHT CombiHT End Fittings (2) (required for use) Replacement seal 1 ea
21.2 x 150 21.2 x 100 21.2 x 50
5 5 5
970150-902 970100-902 970050-902 820400-901 820385-901
970150-906 970100-906 970050-906 820400-901 820385-901
540

ZORBAX Bonus-RP
ZORBAX Bonus-RP HPLC Columns

Excellent peak shape for challenging basic compounds at low and mid pH Unique reversed-phase selectivity Novel bonding technology with embedded polar group and steric protection Usable in 100% aqueous mobile phases
Recommended Column ZORBAX Bonus-RP 1 2 3 pH Range for Optimal Column Life 4 5 6 7 8 9 10 11 12 13
R1 O Si R1 CH 3 Si CH 3 R1 O Si R1 CH 3 Si CH 3 R1 O Si R1 PG R CH 3 PG R CH 3 PG R
Unique Selectivity
The Agilent ZORBAX Bonus-RP column has a polar amide group embedded in a long alkyl chain. This novel bonding reduces interactions between basic compounds and the silica support, improving peak shape for the most difficult basic compounds. Peak shape and column lifetime are further improved by triple endcapping. In addition, diisopropyl side groups provide steric protection against acid hydrolysis for good lifetime at low pH. The Bonus-RP column provides an alternate selectivity to C18 and C8 alkyl bonded phases.
ZORBAX Bonus-RP is Stable at Mid and Low pH

Mid-pH-Triple endcapping of Bonus-RP enhances stability at pH 7. Each 10,000 column volumes is equivalent to approximately one working month.
0.0018 Toluene Plate Height, cm 0.0016 0.0014 0.0012 0.0010 0 5000 10,000 15,000 20,000 25,000 30,000 35,000 Column Volume of Purge Competitive alkyl/amide Bonus-RP
Unique, Polar Alkyl Bonus-RP Bonded Phase Triple Endcapped PG = polar amide group R1 = sterically protecting isopropyl group R = C14 alkyl chain
Columns: 4.6 x 150 mm Mobile Phase: 60% 25 mM phosphate buffer, pH 7.0, 40% ACN Flow Rate: 1.5 mL/min Temperature: 23C
LC and LC/MS
Mid-pH Stability
Low-pH-Sterically protecting side groups provide good low pH stability and longer column lifetime than competitive polar alkyl bonded phases.
0.0055 Toluene Plate Height, cm 0.0056 0.0045 0.0040 0.0035 0.0030 0.0025 0.0020 0 10,000 20,000 Column Volume of Purge 30,000 40,000 Dimethyl-C18/amide Bonus-RP
Low pH Stability
Columns: 4.6 x 150 mm Mobile Phase Aging: 50% MeOH 50% 0.1% TFA Flow Rate: 1.0 mL/min Temperature: 60C Mobile Phase Test: 80% MeOH 20% H2O Flow Rate: 1.0 mL/min Temperature: 23C
541

ZORBAX Bonus-RP
Improved Peak Shape of Basic Compounds Using Bonus-RP
Bonus-RP eliminates peak tailing of these basic compounds in comparison to a typical alkyl C8 bonded phase. In the mid-pH region, residual silanols can interact more strongly with basic compounds to cause peak tailing. The polar group in the Bonus-RP bonded phase eliminates peak tailing of these basic compounds by reducing interactions with residual silanols.
TF 1. 1.8 2. 1.7 3. 2.1

3
Column: 4.6 x 150 mm, 5 m Mobile Phase: 75% 25 mM NH4OAc, pH 5.5, 25% ACN Flow Rate: 1.5 mL/min Temperature: 40C Detection: 254 nm Sample: 1. Doxylamine 2. Chlorpheniramine 3. Triprolidine
8 9 10
5 6 Time (min)
Alkyl-C8
Column:
TF
LC and LC/MS
1. 1.1 2. 1.1 3. 1.1
3 4 Time (min)
4.6 x 150 mm, 5 m P/N 883668-901 Mobile Phase: 80% 25 mM NH4OAc, pH 5.5, 20% ACN Flow Rate: 1.5 mL/min Temperature: 40C Detection: 254 nm Sample: 1. Doxylamine 2. Chlorpheniramine 3. Triprolidine
Bonus-RP P/N 883668-901
542

ZORBAX Bonus-RP
ZORBAX Bonus-RP Provides Alternative Selectivity
Peak elution order can change dramatically when using Bonus-RP. Column: 4.6 x 150 mm Mobile Phase: 75% 25 mM Na Citrate, pH 6, 25% MeOH Flow Rate: 1.0 mL/min Temperature: Ambient Detection: 254 nm Injection vol: 3 L Sample: Cephalosporins 1. cephalexin 2. cephaclor 3. cephuroxime 4. cephoxitin
mAu 15 10 5 0 mAu 15 10 5 0 0 2 4 Time (min) 6 8 10 4 2 2 3 4
B
3
A. Bonus-RP P/N 883668-901
B. Eclipse XDB-C8 P/N 993967-906
ZORBAX Bonus-RP Column Specifications

Bonded Phase Pore Size Surface Area Temp. Limits pH Range Endcapped Carbon Load
ZORBAX Bonus-RP
80
180 m 2/g
60C
2.0-9.0
Triple
9.5%
ZORBAX Bonus-RP Column

Icon Column Description Unit Size (mm) Particle Size (mm) Part No.
LC and LC/MS
Analytical Analytical Rapid Resolution Rapid Resolution Rapid Resolution Narrow Bore Narrow Bore Narrow Bore RR* Narrow Bore RR MicroBore RR MicroBore RR MicroBore RR MicroBore Guard Guard cartridge Guard cartridge Guard hardware kit Guard hardware kit 4/pk 4/pk
4.6 x 250 4.6 x 150 4.6 x 150 4.6 x 100 4.6 x 75 2.1 x 150 2.1 x 50 2.1 x 100 2.1 x 50 1.0 x 150 1.0 x 50 1.0 x 30 1.0 x 13.5 4.6 x 12.5 2.1 x 12.5 4.6 x 12.5 2.1 x 12.5
5 5 3.5 3.5 3.5 5 5 3.5 3.5 3.5 3.5 3.5 5 5 5
880668-901 883668-901 863668-901 864668-901 866668-901 883725-901 861971-901 861768-901 861700-901 863608-901 865608-901 861608-901 5185-5922 820950-928 821125-928 820777-901 820777-901
543

80 ZORBAX Extend-C18
C18 Si O Silica Support
The combination of the unique bidentate structure and double endcapping permits long life at high pH.
C18 Si O
ZORBAX Extend-C18 HPLC Columns

High efficiency and long life at high pHup to pH 11.5 Unique bidentate bonding and double endcapping provides high pH stability More efficiency and better peak shape than polymer-based columns Improve retention, resolution and peak shape of basic compounds High sensitivity for LC/MS separations of peptides
Recommended Column ZORBAX Extend-C18
12
13
The Agilent ZORBAX Extend-C18 column uses a new bidentate C18-C18 column bonding technology to make high pH separations a good choice with a silica-based column. At high pH, non-charged basic compounds will not interact with the silica, so it is possible to do separations with high efficiency, superior peak shape and improved resolution. High pH separations are also the best choice for compounds that are more stable or more soluble in high pH solutions. Some of the mobile phase buffer options for high pH include triethylamine, pyrrolidine, glycine, borate and ammonium hydroxide. Ammonium hydroxide at pH 10.5 is an excellent mobile phase modifier for the LC/MS of peptides and small molecules with improved sensitivity compared with TFA containing mobile phase at low pH. The Extend-C18 column is stable from pH 2-11.5 with good peak shape for all types of compounds. Extend-C18 columns also provide an additional selectivity choice at low pH.*
*Kirkland, J.J., J.B. Adams, Jr., M.A. van Straten and H.A. Claessens, (1998) Analytical Chemistry, 70, 4344 4352.
Long Life at High pH with Extend-C18

At high pH, columns will fail due to silica dissolution. The extended lifetime of ZORBAX Extend-C18 at high pH, in comparison to Waters XTerra MS C18, can be measured by the amount of dissolved silica.
LC and LC/MS
30 ZORBAX Extend-C18 20 Waters XTerra MS C18
Amount of Dissolved Silica (mg)
10
Data provided by H.A. Claessens, Eindhoven University
Column: 4.6 x 150 mm Mobile Phase: 20% Methanol, 80% 0.1 M carbonate buffer, pH 10.0 Flow Rate: 1.0 mL/min Temperature: Ambient
0 0 3 5 9 Volume Eluent (L) 12 15
544

Extend-C18 Provides Good Peak Shape at Low pH
These basic compounds are separated on the Extend-C18 at low pH with excellent peak shape. The Extend-C18 column can be used at high and low pH. Column: ZORBAX Extend-C18 4.6 x 150 mm, 5 m P/N 773450-902 Mobile Phase: 80% 25 mM NaH2PO4, pH 3.0, 20% Methanol Flow Rate: 1.0 mL/min Temperature: 35C Detection: UV 254 nm Sample: 1. Theobromine 2. Dimethylxanthine 3. Theophylline 4. Caffeine
1 3
3 4 Time (min)
Improved Retention, Resolution and Peak Shape of Basic Antihistamines on Extend-C18 at High pH
Pseudoephedrine and scopolamine are difficult to retain at low and mid pH. Pseudoephedrine is often analyzed by ion exchange methods. The Extend-C18 column retains these compounds in a noncharged form at high pH and improves resolution. Column: ZORBAX Extend-C18 4.6 x 150 mm, 5 m P/N 773450-902 Mobile Phase: See figure Flow Rate: 1.0 mL/min Temperature: Ambient Detection: 254 nm
0 4 2,3 1 7
LC and LC/MS
Sample: 1. Maleate 2. Scopolamine 3. Pseudoephedrine 4. Doxylamine 5. Chlorpheniramine 6. Triprolidine 7. Diphenhydramine
pKa 7.6 9.8 9.2 9.1 6.5 9.0
5 Time (min)
pH 7, 30% 20 mM Na2HPO4, 70% MeOH
3 5 1
5 Time (min)
10
pH 11, 30% 20 mM TEA, 70% MeOH
545

1
High Efficiency Separation of Basic Compounds at High pH on Extend-C18

2
The Extend-C18, a silica based column, provides greater efficiency, improved peak shape and faster analysis at high pH in comparison to a polymeric bonded phase. A lower flow rate had to be used for the polymeric column due to a pressure limitation of 180 bar.
3 4 5
10 Time (min)
20
30
Polymeric-Based Column, 4.0 x 250 mm, 5 m Flow Rate: 0.5 mL/min, Pressure: 142 bar
Mobile Phase: 65% 20 mM TEA, pH 11 35% MeOH Temperature: Ambient Detection: UV 254 nm
Sample: 1. Pyridoxine 2. Pyridine 3. N-methylbenzylamine 4. Procainamide 5. N-acetylprocainamide
ZORBAX Extend-C18 Column Specifications

23 5
Bonded Phase
Pore Size
Surface Area
Temp. Limits
pH Range
Endcapped
Carbon Load
ZORBAX Extend-C18
80
180 m2/g
60C*
2.0-11.5
Double
12.5%
*Temperature limits are 60C up to pH 8, 40C for pH 8 - 11.5.
10 Time (min)
15
20
ZORBAX Extend-C18 Column (80 )

Icon Column Description Unit Size (mm) Particle Size (m) Part Number
Extend-C18, 4.6 x 250 mm, 5 m P/N 770450-902 Flow Rate: 1.0 mL/min, Pressure: 213 bar
Analytical Analytical Analytical Rapid Resolution Rapid Resolution Rapid Resolution Rapid Resolution Solvent Saver Plus Solvent Saver Plus Narrow Bore Narrow Bore Narrow Bore RR* Narrow Bore RR MicroBore RR MicroBore RR MicroBore RR MicroBore Guard Guard cartridge Guard cartridge Guard hardware kit Guard hardware kit 4/pk 4/pk
4.6 x 250 4.6 x 150 4.6 x 50 4.6 x 150 4.6 x 100 4.6 x 75 4.6 x 50 3.0 x 150 3.0 x 50 2.1 x 150 2.1 x 50 2.1 x 100 2.1 x 50 1.0 x 150 1.0 x 50 1.0 x 30 1.0 x 13.5 4.6 x 12.5 2.1 x 12.5 4.6 x 12.5 2.1 x 12.5
5 5 5 3.5 3.5 3.5 3.5 3.5 3.5 5 5 3.5 3.5 3.5 3.5 3.5 5 5 5
770450-902 773450-902 746450-902 763953-902 764953-902 766953-902 735953-902 763954-302 735954-302 773700-902 760450-902 761753-902 735700-902 763600-902 765600-902 761600-902 5185-5923 820950-930 821125-930 820777-901 820777-901
LC and LC/MS
*RR: Rapid Resolution 3.5 m columns. Configurations not shown are available upon request. 300 Extend-C18 columns are also available for peptide separations. See page 549 for descriptions and ordering information
546

ZORBAX 300Extend-C18 HPLC Columns for BioAnalytical Chemistry

Rugged, high and low pH separations of polypeptides and peptides from pH 2-11.5 Different selectivity possible at high and low pH High efficiency and good recovery of hydrophobic peptides at high pH Ideal for LC/MS with ammonium hydroxide modified mobile phase
Recommended Column ZORBAX Extend-C18 1 2 3 pH Range for Optimal Column Life 4 5 6 7 8 9 10 11 12 13
C18 Si O Silica Support
C18 Si O
New Bidentate C18-C18 Bonding for Extend-C18 Bonded Phase
ZORBAX 300Extend-C18 is a wide-pore HPLC column for the high efficiency separations of peptides from pH 2-11.5. The unique bidentate bonded phase provides excellent lifetime and reproducibility at high and low pH. At high pH, retention and selectivity of peptides and polypeptides can change dramatically as a result of changes in charge on molecules. Excellent recoveries of hydrophobic polypeptides have been achieved at room temperature and high pH. LC/MS sensitivity of peptides and polypeptides can also be improved at high pH using a simple ammonium hydroxide containing mobile phase. The 300Extend-C18 column can be used at high and low pHfrom pH 2-11.5. This chart shows that the 300Extend-C18 has the needed stability at low pH for long-term reproducible separations. Therefore one wide-pore column can be used for selectivity optimization at low and high pH with both TFA and ammonium hydroxide mobile phases. Columns: Column Aging: Flow Rate: Temperature: Column Test: Flow Rate: Temperature: Test Solute: 4.6 x 150 mm, 5 m 50% Methanol : 50% Water + 1% TFA 1.5 mL/min, continuous 90C 60% Methanol : 40% Water 1.5 mL/min Ambient Toluene
Fraction of Toluene k Remaining
1.0
0.8
0.6
0.4 Stablebond SB-C18 (Diisobutyl-C18) Extend-C18 (Bidentate-C18) 0.2 Rx-C18 (Dimethyl-C18)
LC and LC/MS
0.0 0 5000 10000 15000 20000 25000 30000 Column Volumes of Purge
Extend-C18 and StableBond SB-C18 are Stable at Low pH

8 1-Chloro-4-nitrobenzene 6 Trimipramine
Column: ZORBAX Extend-C18, 4.6 x 150 mm Mobile Phase: 20% 20 mm NH4OH, pH 10.5, 80% Methanol Flow Rate: 1.5 mL/min Aging at 24C, tests at 40C Each 10,000 column volumes is approximately one working month.
k V alues
10000
20000
30000
40000
50000
Column Volumes of Mobile Phase
Long Life at High pH with Extend-C18
547

Both small and large peptides demonstrate selectivity changes at high and low pH. At high pH, due to a change in charge, all three Angiotensins can be resolved. In addition, the spectral clarity of Angiotensin I is dramatically improved at high pH with the ammonium hydroxide mobile phase. The Extend-C18 column can also be used for the analysis of small peptides at high pH.
LC/MS Analysis of Angiotensin on Extend-C18
12.050
Column:
Angiotensin I
433.0 649.5
5.0E6 TIC (200-1500 m/z) 4.0E6 3.0E6 2.0E6 1.0E6 0 0 2.5 5 7.5 10
100 80
13.261
227.1
+3
+2
Max: 10889
AII+AIII
60
250.9
844.8
1277.9 1326.3 1373.3
340.3 398.0
720.3 767.3
1025.2
1179.1
AI
659.7
40 20 0
12.5
min
500
1000
m/z
A. Acidic Conditions: A: 0.1% TFA in water B: 0.085% TFA in 80% acetonitrile (ACN)
ZORBAX Extend-C18 2.1 x 150 mm, 5 m P/N 773700-902 Flow Rate: 0.2 mL/min Temperature: 35C Mobile Phase: As indicated Gradient: 15-50% B in 15 min. LC/MS: Pos. Ion ESI - Vf 70V, Vcap 4.5 kV, N2- 35 psi, 12 L/min., 325C Sample: Angiotensin I, II, III 2.5 L sample (50 pmol each) Angiotensin I: Asp-Arg-Val-Tyr-Ile-HisPro-Phe-His-Leu Angiotensin II: Asp-Arg-Val-Tyr-Ile-HisPro-Phe Angiotensin III: Arg-Val-Tyr-Ile-His-Pro-Phe
5.0E7 TIC (200-1500 m/z) 4.0E7 3.0E7 2.0E7 1.0E7 0 0 2.5 5 7.5 10 12.5 min
100
Angiotensin I
649.0
AIII
9.621
80
433.0
+2
Max: 367225
AII
5.499 8.477
60 40 20 0
AI
+3
659.8 1296.6
+1
m/z
500
1000
B. Basic Conditions: A: 10 mM NH4OH in water B: 10 mM NH4OH in 80% ACN Boyes, B.E., Separation and Analysis of Peptides at High pH Using RP-HPLC/ESI-MS, 4th WCBP, San Francisco, CA Jan. 2000.
LC and LC/MS
1468.8
458.0
548

At high pH and room temperature the hydrophobic amyloid peptides show better recovery and a different selectivity than at low pH. At low pH, elevated temperature is necessary to achieve the same recovery as at high pH.
TFA Conditions, 25C A - 0.1% TF A in water B - 0.085% TF A in 80% ACN Gradient: 33 - 45% B in 30 min.
ZORBAX 300Extend-C18 Column Specifications

Bonded Phase Pore Size Surface Area Temp. Limits pH Range 2.0-11.5 Endcapped Double Carbon Load 12.5%
A (1-38)
A (1-40)
A (1-42/43)
ZORBAX 300Extend-C18 80
180 m2/g 60C*
*Temperature limits are 60C up to pH 8, 40C for pH 8-11.5.
ZORBAX 300Extend-C18 Column

Icon Column Description Analytical Analytical Rapid Resolution Rapid Resolution Rapid Resolution Narrow Bore RR* Narrow Bore RR Narrow Bore RR Guard cartridges Guard cartridges Guard hardware kit Guard hardware kit Capillary Glass-Lined Columns Capillary RR Capillary RR Capillary RR Capillary RR Replacement screens 10/pk 0.3 x 150 0.3 x 100 0.3 x 75 0.3 x 50 3.5 3.5 3.5 3.5 5065-4464 5065-4465 5065-4466 5065-4467 5065-4427
*RR: Rapid Resolution 3.5 m columns Configurations not shown are available upon request.
TFA Conditions, 80C A - 0.1% TF A in water B - 0.085% TF A in 80% ACN A (1-42) Gradient: 29-41% B in 30 min. A (1-43) A (1-40)
Unit
Size (mm) 4.6 x 250 4.6 x 150 4.6 x 150 4.6 x 100 4.6 x 50 2.1 x 150 2.1 x 100 2.1 x 50
Particle Size (m) 5 5 3.5 3.5 3.5 3.5 3.5 3.5 5 5
Part No. 770995-902 773995-902 763973-902 761973-902 765973-902 763750-902 761775-902 765750-902 820950-932 821125-932 820777-901 820777-901
0 5
A (1-38)
NH 4OH Conditions, 25C A - 20 mM NH4OH in water B - 20 mM NH4OH in 80% ACN Gradient: 26-38% B in 30 min. A (1-42) A (1-40) A (1-38) A (1-43)
4/pk 4/pk
4.6 x 12.5 2.1 x 12.5 4.6 x 12.5 2.1 x 12.5
10 15 Time (min)
20
25
High pH can be used for separating hydrophobic or other low solubility peptides
LC and LC/MS
549

ZORBAX Rx HPLC Columns
ZORBAX Rx HPLC Columns for Analytical Chemistry

Rx-C18 is recommended for alternate selectivity at low pH relative to StableBond SB-C18; for higher temperature applications, StableBond is recommended. This column has a higher carbon load than SB-C18 columns (12% vs. 10%). Rx-C18 offers high stability and good peak shape for low pH applications Rx-C18 is manufactured using dimethyloctadecylsilane, is non-endcapped, and provides excellent stability up to pH 9.
Recommended Column ZORBAX Rx-C18 1 2 3 pH Range for Optimal Column Life 4 5 6 7 8 9 10 11 12 13
Rx-C8 is the same product as SB-C8

Recommended Column ZORBAX Rx-C8
12
13
An Rx-C18 column is used for this USP analysis of diazepam and the internal standard ethylparaben. The Solvent Saver 3.0 mm id Rx-C18 column reduces solvent usage by 60% over what would be used if the analysis were done on a 4.6 x 250 mm column. Column: ZORBAX Rx-C18 3.0 x 250 mm, 5 m P/N 880967-302 35% H2O 65% MeOH 0.5 mL/min 1. Ethylparaben 2. Diazepam
Mobile Phase:
LC and LC/MS
Flow Rate: Sample:

0.0 2.5 5.0 Time (min) 7.5
Analysis of Diazepam on Rx-C18
ZORBAX Rx Column Specifications

Bonded Phase ZORBAX Rx-C18 ZORBAX Rx-C8 Pore Size 80 80 Surface Area 180 m2/g 180 m2/g Temp. Limits 60C 80C pH Range 2.0 - 9.0 1.0 - 8.0 Endcapped No No Carbon Load 12% 5.5%
550

ZORBAX Rx HPLC Columns
ZORBAX Rx Column
Icon Column Description
Standard Columns
Unit
Size (mm) 21.2 x 250 9.4 x 250 4.6 x 250 4.6 x 150 4.6 x 150 4.6 x 100 4.6 x 75 3.0 x 250 3.0 x 150 2.1 x 150 2.1 x 100
Particle Size (m) 7 5 5 5 3.5 3.5 3.5 5 5 5 3.5 7 5 5
Rx-C18 880967-102 880967-202 880967-902 883967-902 863967-902 861967-902 866967-902 880967-302 883967-302 883700-902 861767-902 820675-115 820950-914 821125-926 840140-901 820777-901 820777-901
Rx-C8 880967-106 880967-201 880967-901 883967-901 863953-906 861953-906 866953-906 880975-306 883975-306 883700-906 861753-906 820675-115 820950-913 821125-926 840140-901 820777-901 820777-901
Preparative Semi-Preparative Analytical Analytical Rapid Resolution Rapid Resolution Rapid Resolution Solvent Saver Solvent Saver Narrow Bore Narrow Bore RR* Guard cartridge packed with Rx/SB-C8 Guard cartridge Guard cartridge packed with Eclipse XDB-C8 Guard hardware kit Guard hardware kit Guard hardware kit 2/pk 4/pk 4/pk
9.4 x 15 4.6 x 12.5 2.1 x 12.5 9.4 x 15 4.6 x 12.5 2.1 x 12.5
*RR: Rapid Resolution 3.5 m columns.
Note: Rx-C8 is the same product as StableBond SB-C8. Configurations not shown are available upon request.
LC and LC/MS
551

High-Throughout/CombiHT
ZORBAX CombiHT Preparative Cartridge Columns

Fast preparative separations in the 15-50 mg range Three different column lengths50, 100, 150 mm Finger tight connections seal up to 7000 psi CombiHT preparative columns are designed to scale up rapid analytical separations to sample loads of 15-50 mg. CombiHT columns use 5 m packings for high efficiency and provide direct scale up from 50-150 mm analytical columns. These columns are available with reusable, finger tight endfittings so only the column barrel is replaced. CombiHT C8 and C18 columns are available in the two most popular ZORBAX bonded-phase types to optimize selectivity, StableBond for low pH and Eclipse XDB for mid-pH but can be packed with any ZORBAX phase on request. Flow Rate Conversion To determine the optimum flow rate for your 21.2 mm i.d. column, use the following equation: F2 = F1 x (ID2 /ID1)2
0 10 20 Time (min) 30
A. 35 mg Prep Sample CombiHT SB-C18 21.2 x 50 mm, 5 m P/N 870050-902 10-40% B in 20 min 30 mL/min A: 0.1 % TFA in H20 B: 0.1 % TFA in ACN
For example,
4.6 mm id 0.5 mL/min 1.0 mL/min 1.5 mL/min 21.2 mm id 10.5 mL/min 21 mL/min 31.5 mL/min
Data provided by Hoffmann LaRoche, Ted Lambrose
LC and LC/MS
B. Purity Check Rapid Resolution SB-C18 4.6 x 50 mm, 3.5 m P/N 835975-902 10-50% B in 20 min 3 mL/min A: 0.1 % TFA in H20 B: 0.1 % TFA in ACN
5 Time (min)
10
Gradient Preparative High Throughout HPLC
The CombiHT column is used to separate and isolate a compound of interest, while the matching analytical column confirms the peak was isolated with high purity.
552

CombiHT/Semi-Prep
ZORBAX CombiHT Preparative Cartridge Column (endfittings required)
Icon Column Description Size (mm) Particle Size (m) SB-C18 USP L1 SB-C8 USP L7 Eclipse XDB-C18 USP L1 Eclipse XDB-C8 USP L7
CombiHT CombiHT CombiHT CombiHT end fittings (required for use) Replacement seal (1 ea)
21.2 x 150 21.2 x 100 21.2 x 50
5 5 5
870150-902 870100-902 870050-902 820400-901 820385-901
870150-906 870100-906 870050-906 820400-901 820385-901
970150-902 970100-902 970050-902 820400-901 820385-901
970150-906 970100-906 970050-906 820400-901 820385-901
ZORBAX Semi-Preparative Columns

Icon Column Description Semi-Preparative Semi-Preparative Semi-Preparative Guard cartridge, 2/pk Guard hardware kit Size (mm) 9.4 x 150 9.4 x 100 9.4 x 50 9.4 x 15 Particle Size (m) 5 5 5 7 SB-C18 USP L1 883975-902 884975-202 846975-202 820675-115 840140-901
LC and LC/MS
553

LC/MS Columns
ZORBAX LC/MS HPLC Columns and Cartridge Columns

Fast analysis for high speed LC/MS with Rapid Resolution cartridges Narrow bore and MicroBore columns for maximum sensitivity with Electrospray Ionization (ESI) Additional column dimensions for higher sample loads with APCI Many bonded phases available Selecting a column for LC/MS involves three simple choices: column internal diameter, column length and bonded phase. ZORBAX Rapid Resolution, ultra-short, narrow-bore cartridge columns (2.1 x 15 and 2.1 x 30 mm) are often a first choice for LC/MS applications because these columns provide the fastest analysis time and good sensitivity with ESI detection. ZORBAX short, narrow-bore (2.1 x 50 mm) and MicroBore (1.0 x 50 mm) columns are also good choices for ESI/MS because their optimum flow rates are from 50-200 L/min, providing additional sample resolution while still keeping analysis times very short. Even longer lengths are available for very complex samples. The 4.6 x 15 mm and 30 mm cartridges, as well as the longer columns, provide the same resolution choices and are an ideal choice for APCI detectors where the low flow rate is not required. ZORBAX StableBond and Eclipse XDB-C18 and C8 bonded phases are available in all configurations and additional bonded phases can be packed on request. StableBond columns demonstrate superior low pH stability and are ideal for formic acid and TFA containing mobile phases. Eclipse XDB bonded phases are a great choice with acetic acid mobile phases or for quick compound screening over a wide pH range.
LC and LC/MS
554

LC/MS Columns
Column: ZORBAX StableBond SB-C18 Mobile Phase: 85% Water with 0.1% TFA 15% Acetonitrile Flow Rate: 1.0 mL/min Temperature: 35C Sample: 1. Phenylalanine 2. 5-Benzyl-3,6-dioxo-2-piperazine acetic acid 3. Asp-phe 4. Aspartame
1 1
3 4
3 4
3 4
2 Time (min)
Reduce Analysis Time By 70% Using Short LC/MS Cartridge Columns
Column: Rapid Resolution SB-C18, 3.5 m Mobile Phase: Gradient: 5-35% B in 6 min A: Water with 0.2% formic acid B: Methanol with 0.2% formic acid Temperature: 35C Sample: 2 L Organic Acids 1. Gallic 2. Protocatechuic 3. Hydrocaffeic 4. Gentisic 5. Vanillic 6. Syringic
mAU 150 2
100 3
2 50 1 3 5 1 4 4 0 5 6 6
LC and LC/MS
Maximize Sensitivity with Narrow-Bore LC/MS Cartridges
555

ZORBAX Rapid Resolution Columns
1 4
2 3
ZORBAX Rapid Resolution 3.5 m HPLC Columns

6
3 Time (min)
Reduce analysis time and solvent usage and increase sample throughput High efficiency in short and ultra-short column lengths Available in analytical (4.6 mm) and narrow-bore (2.1 mm) id Comparable lifetime to 5 m columns Agilent ZORBAX Rapid Resolution columns, with a 3.5 m particle size, reduce analysis time and increase sample throughput for any application when compared to 5 m columns. Rapid Resolution columns are available from 15-150 mm, in both 4.6 and 2.1 mm internal diameters, so the best configuration is available for high-throughput, LC/MS, combinatorial chemistry and rapid analytical applications. ZORBAX Rapid Resolution particles have superior mechanical strength, so every Rapid Resolution column has an extremely stable packed bed and provides a comparable lifetime to 5 m columns. ZORBAX Rapid Resolution columns are available in all of the modern ZORBAX bonded phasesStableBond, Eclipse XDB, Bonus-RP and Extend-C18.
Bonus-RP, 4.6 x 150 mm, 5 m P/N 883668-901 Flow Rate: 1.5 mL/min
1 4 2 3
2 Time (min)
Rapid Resolution Bonus-RP 4.6 x 75 mm, 3.5 m P/N 866668-901 Flow Rate: 1.5 mL/min
4 1 2 3
LC and LC/MS
Column: ZORBAX Bonus-RP Mobile Phase: 80% 25 mM NH4Ac, pH 5.5 20% ACN Temperature: 40C Detection: 254 nm Sample: 1. Caffeine 2. Doxylamine 3. Chlorpheniramine 4. Triprolidine
1 2 Time (min)
Narrow Bore Rapid Resolution Bonus-RP 2.1 x 50 mm, 3.5 m P/N 861700-901 Flow Rate: 0.3 mL/min
Rapid Resolution Columns Reduce Analysis Time While Maintaining Resolution
556

Rapid Resolution 4.6 x 75 mm, 3.5 m columns are a good choice for initial method development because they allow you to quickly determine if a given column and mobile phase combination can produce the desired separation. Column: ZORBAX StableBond SB-C18 4.6 x 75 mm, 3.5 m P/N 866953-902 A: 25 mM NaH2PO4, pH 3.0 B: MeOH 2.0 mL/min 35C UV 254 nm 1. Diltiazem 2. Dipyridamole 3. Nifedipine 4. Lidoflazine 5. Flunarizine
1
Mobile Phase: Flow Rate: Temperature: Detection: Sample:
5 4
0.0
0.5
1.0
1.5 2.0 Time (min)
2.5
3.0
0.0
0.5
1.0
1.5 2.0 Time (min)
2.5
3.0
0.0
0.5
1.0
1.5 2.0 Time (min)
2.5
3.0
Run Method Development Chromatograms on Rapid Resolution Columns
ZORBAX Rapid Resolution LC/MS Cartridge Columns (requires Hardware Kit P/N 820222-901)
Column Description Rapid Resolution Cartridge Rapid Resolution Cartridge Rapid Resolution Cartridge Rapid Resolution Cartridge Rapid Resolution Cartridge Rapid Resolution Cartridge Rapid Resolution Cartridge Rapid Resolution Cartridge High throughput cartridge column hardware kit Replacement seals 2/pk 3/pk 3/pk 3/pk 3/pk Size (mm) 4.6 x 30 4.6 x 30 4.6 x 15 4.6 x 15 2.1 x 30 2.1 x 30 2.1 x 15 2.1 x 15 Particle Size (m) 3.5 3.5 3.5 3.5 3.5 3.5 3.5 3.5 SB-C18 USP L1 833975-902 833975-932 831975-902 831975-932 873700-902 873700-932 875700-902 875700-932 820222-901 820370-901 SB-C8 USP L7 833975-906 833975-936 831975-906 831975-936 873700-906 873700-936 875700-906 875700-936 820222-901 820370-901 Eclipse XDB-C18 USP L1 Eclipse XDB-C8 USP L7
Icon
Unit
933975-902 933975-906 933975-932 933975-936 931975-902 931975-906 931975-932 931975-936 973700-902 973700-906 973700-932 973700-936 975700-902 975700-906 975700-932 975700-936 820222-901 820222-901 820370-901 820370-901
LC and LC
557

ZORBAX Rapid Resolution and LC/MS Columns
Column Icon Description Rapid Resolution Rapid Resolution Rapid Resolution Rapid Resolution Solvent Saver Plus Solvent Saver Plus Narrow Bore Narrow Bore Narrow Bore RR* Narrow Bore RR Narrow Bore RR MicroBore RR MicroBore RR MicroBore RR MicroBore Guard Size (mm) Particle SB-C18 Size (m) USP L1 SB-C8 USP L7 SB-CN USP L10 SB-C3 SB-Phenyl SB-Aq USP L11 863953-912 863953-914 861953-912 861953-914 866953-912 866953-914 835975-912 835975-914 863954-312
ZORBAX StableBond Columns
4.6 x 150 3.5 4.6 x 100 3.5 4.6 x 75 4.6 x 50 3.5 3.5
863953-902 863953-906 863953-905 861953-902 861953-906 861953-905 866953-902 866953-906 866953-905 835975-902 835975-906 835975-905 863954-302 863954-306 863954-305 861954-306
3.0 x 150 3.5 3.0 x 100 3.5 2.1 x 150 5 2.1 x 50 5 2.1 x 150 3.5 2.1 x 100 3.5 2.1 x 50 1.0 x 50 1.0 x 30 3.5 3.5 3.5 7 1.0 x 150 3.5
883700-922 883700-906 883700-905 883700-909 883700-912 860975-902 860975-906 860975-905 860975-909 860975-912 860975-914 830990-902 830990-906 861753-902 861753-906 861753-905 871700-902 871700-906 863600-902 863600-906 863600-905 865600-902 865600-906 861600-902 861600-906 5185-5920 5185-5920 820675-115 820950-922 820950-917 820950-933 821125-924 821125-926 821125-933 840140-901 820777-901 820777-901 820777-901 820777-901 820777-901 820777-901 820675-115 820675-115 820675-124 820950-920 820950-915 820950-916 821125-926 821125-926 821125-924 840140-901 840140-901 840140-901 820777-901 820777-901 820777-901 820777-901 820777-901 820777-901 830990-914 861753-912 861753-914 871700-914
1.0 x 13.5 5
Guard cartridge, 2/pk 9.4 x 15
Guard cartridge, 4/pk 4.6 x 12.5 5 Guard cartridge, 4/pk 2.1 x 12.5 5
LC and LC/MS
Guard hardware kit Guard hardware kit Guard hardware kit
9.4 x 15 4.6 x 12.5 2.1 x 12.5
*RR: Rapid resolution 3.5 m columns Configurations not shown are available upon request. For more information on these and other columns consult the Agilent web site at www.agilent.com.
558

ZORBAX Rapid Resolution and LC/MS Columns (continued)
Column Description Rapid Resolution Rapid Resolution Rapid Resolution Rapid Resolution Narrow Bore Narrow Bore Narrow Bore RR Narrow Bore RR MicroBore RR MicroBore RR MicroBore RR Size (mm) Particle Size (m) Rx-C18 863967-902 861967-902 866967-902 Eclipse XDB-C18 USP L1 963967-902 961967-902 966967-902 935967-902 963954-302 993700-902 960967-902 883700-902 861767-902 883700-906 861753-906 930990-902 961753-902 971700-902 963600-902 965699-902 961600-902 Eclipse XDB-C8 USP L7 963967-906 961967-906 966967-906 935967-906 963954-306 993700-906 960967-906 930990-906 961753-906 971700-906 963600-906 965699-906 961600-906 861768-901 861700-901 863608-901 865608-901 861608-901
*RR Rapid Resolution 3.5 m columns.
Rx-C8 863953-906 861953-906 866953-906
Eclipse XDB-Phenyl USP L11 Bonus-RP 963967-912 966967-912 935967-912 963954-312 993700-912 960967-912 883725-901 861971-901 863668-901 864668-901 866668-901
ZORBAX Rx, Eclipse XDB and Bonus-RP Columns
4.6 x 150 3.5 4.6 x 100 3.5 4.6 x 75 4.6 x 50 3.5 3.5
Solvent Saver Plus 3.0 x 150 3.5 2.1 x 150 5 2.1 x 50 5
Narrow Bore RR* 2.1 x 150 3.5 2.1 x 100 3.5 2.1 x 50 1.0 x 50 1.0 x 30 3.5 3.5 3.5 1.0 x 150 3.5
Column Description
ZORBAX Extend-C18 Columns
Size (mm) 4.6 x 150 4.6 x 100 4.6 x 75 4.6 x 50 2.1 x 150 2.1 x 50 2.1 x 100 2.1 x 50 1.0 x 150 1.0 x 50 1.0 x 30
Particle Size (m) 3.5 3.5 3.5 3.5 5 5 3.5 3.5 3.5 3.5 3.5
Extend-C18
LC and L
Rapid Resolution Rapid Resolution Rapid Resolution Rapid Resolution Narrow Bore Narrow Bore Narrow Bore RR* Narrow Bore RR MicroBore RR MicroBore RR MicroBore RR
763953-902 764953-902 766953-902 735953-902 773700-902 760450-902 761753-902 735700-902 763600-902 765600-902 761600-902
*RR Rapid Resolution 3.5 m columns.
559

MicroBore and Capillary Columns
Agilent MicroBore and Capillary Columns

High sensitivity for smallest sample sizes Compatibility with several LC/MS interfaces Several internal diameters1.0 mm, 0.5 mm, 0.3 mm Variety of bonded phases MicroBore (1 mm) and capillary columns (0.5, 0.3 mm) are often the best choice when sample sizes are limited. MicroBore columns will improve limits of detection 5X over 2.1 mm id columns, and capillary columns can decrease detection limits 20-50X relative to 2.1 mm id columns when the same sample size is used. This increase in sensitivity can be critical. MicroBore and capillary columns use very low flow ratesfrom 1-50 L/min. Therefore, these columns are ideal for use with detectors requiring the lowest flow rates, such as the particle beam and electrospray LC/MS detectors. Agilent MicroBore and capillary columns are available in three different internal diameters and several lengths, so the ideal configuration is available for your sample limited applications. The 1.0 mm MicroBore columns are packed in stainless steel, and the 0.3 and 0.5 mm capillary columns are packed into glass-lined, stainless steel columns. These columns perform optimally with HPLC systems purchased or adjusted for microbore and capillary column use. Many bonded phases are available, including StableBond SB-C18 and SB-C8, Eclipse XDB-C18 and XDB-C8, Bonus-RP, Extend-C18, 300 Extend-C18, 300SB-C18, 300 SB-C8, and Hypersil ODS. Note: Check for availability of Poroshell capillary columns in the Spring of 2002.
LC and LC/MS
560

MicroBore and Capillary Columns
ZORBAX MicroBore HPLC Column
Size (mm) 1.0 x 150 1.0 x 50 1.0 x 30 1.0 x 13.5 (Guards) Particle SB-C18 Size (m) USP L1 3.5 3.5 3.5 5 SB-C8 USP L7 Eclipse XDB-C18 USP L7 Eclipse XDB-C8 USP L7
300SB-C18
300SB-C8
Bonus-RP
Extend-C18
863600-902 863600-906 863630-902 863630-906 963600-902 963600-906 863608-901 763600-902 865600-902 865600-906 865630-902 865630-906 965600-902 965600-906 865608-901 765600-902 861600-902 861600-906 5185-5920 5185-5920 5185-5920 5185-5920 961600-902 961600-906 861608-901 761600-902 5185-5921 5185-5921 5185-5922 5185-5923
Agilent HPLC Capillary Column (glass-lined)

Column Description Capillary Capillary Capillary Capillary RR Capillary Capillary Capillary Capillary RR Capillary RR Capillary RR Capillary RR Replacement screens, 10/pk Size (mm) 0.5 x 250 0.5 x 150 0.5 x 35 0.5 x 35 0.3 x 250 0.3 x 150 0.3 x 35 0.3 x 150 0.3 x 100 0.3 x 75 0.3 x 50 Particle Size (m) SB-C18 5 5 5 3.5 3.5 5 5 5 3.5 3.5 3.5 3.5 Eclipse XDB-C18 300SB-C18 300SB-C8 300Extend-C18 Hypersil ODS 5064-8278 5064-8289 5064-8282 5064-8275+ 5064-8299+ 5064-8276 5064-8290 5065-4460 5065-4461 5065-4462 5065-4463 5065-4427
* RR: Rapid Resolution 3.5m columns + 3m particles
5064-8258 5064-8286 5064-8266 5064-8256 5064-8287 5064-8264 5064-8254 5064-8296 5064-8294 5064-8262 5064-8288 5064-8268 5064-8260 5064-8298 5065-4459 5064-8257 5064-8269 5064-8265 5064-8255 5064-8291 5064-8263 5064-8253 5064-8297 5064-8295 5064-8261 5064-8271 5064-8267 5064-8259 5064-8270 5064-8300 5065-4427 5065-4427 5065-4427 5065-4464 5065-4465 5065-4466 5065-4467
Capillary RR* 0.5 x 150
5064-8279 5064-8277+
LC and LC/MS
561

ZORBAX Original Reversed-Phase Columns
ZORBAX Original Reversed-Phase HPLC Columns

High surface area (300 m2/g, 20% carbon load for ODS(C18)) gives added retention Uses the original ZORBAX SIL support, monolayer silane bonding, and single endcapping for deactivation of silanol groups. High-strength porous silica microsphere technology allows high pressure packing and high plate counts (16,000-20,000 plates/4.6 x 250 mm column) Low backpressure due to narrow particle size distribution In-house silica manufacturing ensures reproducibility Examples include oil-soluble vitamins, steroids, cephalosporins Used in many established methods
ZORBAX Original Reversed-Phase Column Specifications

Bonded Phase, 70 Surface Area Temp. Limits pH Range* End-capped Carbon Load
ZORBAX ODS (C18) ZORBAX C8 ZORBAX Phenyl ZORBAX CN ZORBAX TMS
300 m2/g 300 300 300 m2/g m2/g m2/g
60C 60C 60C 60C 60C
2.0 - 8.0 2.0 - 8.0 2.0 - 8.0 2.0 - 8.0 2.0 - 7.0
Yes Yes Yes Yes N/A
20% 12% 12% 7% 4%
300 m2/g
* At pH 6-8, highest column stability for all silica-based columns is obtained by operating at temperatures <40C using low buffer concentrations in the range of 0.01-0.02 M.
ZORBAX Original Reversed-Phase Columns*
LC and LC/MS
Description
Standard Columns
Size (mm) 21.2 x 250 9.4 x 250 4.6 x 250 4.6 x 250 4.6 x 150 3.0 x 250 3.0 x 150 4.6 x 12.5 9.4 x 15
Particle Size (m) 7.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 7.0
ODS (C18) 880952-102 880952-202 880952-702 884950-543 883952-702 880952-302 883952-302 820950-902 820675-115 820777-901 840140-901
C8 880952-106 880952-206 880952-706 883952-706
PHENYL
CN 880952-105
TMS
Preparative Semi-preparative Analytical Analytical, non-endcapped Analytical Solvent saver Solvent saver Guard cartridges Guard cartridges Hardware kit (4.6 mm id) Hardware kit (9.4 mm id)
880952-212 880952-712 883952-712
880952-205 884950-507 884950-526 880952-710 883952-710
820950-906 820675-115 820777-901 840140-901
820950-912 820675-115 820777-901 840140-901
820950-905 820675-124 820777-901 840140-901
820950-905 820675-124 820777-901 840140-901
* Configurations not shown are available by special products quote (see custom column ordering page 595).
Note: Guard cartridges require hardware kits.
562

ZORBAX Rx-SIL (RP)
ZORBAX Rx-SIL HPLC Columns (Reversed-Phase Mode)

Bare silica can be used in the reversed phase mode with high concentrations of organic modifier for separating hydrophilic compounds The high purity of ZORBAX Rx-SIL reduces tailing for basic compounds Urea Analysis Operating Conditions: Column: ZORBAX Rx-SIL, 4.6 x 150 mm (P/N 883975-901) Mobile Phase: ACN: K2HPO4, 20 mM pH 7.2 (90:10) Detection: UV 210 nm Flow: 1 mL/min Temperature: ambient
Note: Rx-SIL columns are shipped containing normal phase solvent. They must be flushed with a miscible solvent such as IPA before equilibrating with aqueous mobile phase.
Cough Syrup Analysis Operating Conditions: HPLC System: Agilent 1100 with quaternary pump Column: ZORBAX Rx-SIL, 4.6 x 150 mm (P/N 883975-901) Mobile Phase: MeOH: K2HPO4, 20 mM pH 7 (69:31) Detection: UV 217 nm Flow: 2 mL/min Temperature: ambient Note: Part numbers and specifications for ZORBAX Rx-SIL can be found on page 574.
LC and LC/MS
Note: Rx-SIL columns are shipped containing normal phase solvent. They must be flushed with a miscible solvent such as IPA before equilibrating with aqueous mobile phase.
563

Hypersil Reversed-Phase Columns
Additional Reversed-Phase HPLC Columns

In addition to ZORBAX brand HPLC columns, Agilent Technologies packs columns with a variety of excellent reversed-phase sorbents from other manufacturers. These packings have been carefully evaluated by Agilent to meet rigorous quality assurance standards. Agilent's automated and optimized column packing stations and ISO-regulated procedures guarantee high reproducibility.
Hypersil Reversed-Phase HPLC Columns

Hypersil BDS is both base deactivated and endcapped to improve peak shapes of basic solutes by minimizing interaction with unreacted silanols. Hypersil ODS, a very popular packing, adds a monolayer of octadecyl silane (C18) to the Hypersil Silica support. Columns are endcapped to reduce silanol interaction. For less retention with similar selectivity, Hypersil MOS columns provide a monolayer of dimethyloctylsilane (C8 alkyl chain). Agilent's Hypersil columns are offered in standard compression fitting columns and in Agilent's convenient and economical cartridge design. Applications BDS: Basic drugs, explosives, antibiotics ODS: Non-polars, lipophilic compounds Availability 3 and 5 m particle sizes; pore sizes 120 (ODS, MOS), 130 (BDS)
LC and LC/MS
564

Hypersil Columns with Column Identification Module (CIM)
Do more efficient LC column tracking, with less effort. Attached to an LC column, the column identification module (CIM) communicates with the Agilent 1100 Series control module and Agilent ChemStation through a built-in read/write device on the 1100 temperature controlled column compartment. Column usage data including serial and batch numbers, dimensions, particle size, packing material and number of injections is recorded electronically in the module (pre-recorded before shipment of Agilent's CIM HPLC columns). You can also record method, plate number, installation date, operator name and other data as needed. With constant updating, column information is always current. Automatic column tracking enhances the Agilent 1100 Series features for meeting GLP, cGMP and quality standard requirements, and for achieving fast trouble-free validation.
Column with Installed CIM Module
CIM Cartridge Columns

Icon Description Hypersil ODS cartridge 5 m, 4 x 125 mm Hypersil ODS cartridge 5 m, 4 x 125 mm Hypersil BDS-C18 cartridge 3 m, 4 x 100 mm Hypersil BDS-C18 cartridge 3 m, 4 x 250 mm Agilent cartridge holder Part No. 7982618-564 7982618-584 79826BD-354 79826BD-584 5021-1845
CIM Standard Fitting Columns

Description Hypersil ODS, 5 m, 4.6 x 100 mm Hypersil ODS 5 m, 2.1 x 100 mm Part No. 7981618-554 7981618-552
LC and LC/MS
Column Identification Module (CIM)

Description Column identification module Unit 3/pk Part No. 5062-8588
565

Hypersil Cartridge Column*
Icon Size (mm) Particle Size (m) Stationary Phase Part No.
4.6 x 250 4.6 x 150 4.0 x 250 4.0 x 250 4.0 x 125 4.0 x 100 4.0 x 100 4.0 x 75 3.0 x 125 2.0 x 250 2.0 x 125 2.0 x 125 4.0 x 250 4.0 x 125 4.0 x 75 2.0 x 250 2.0 x 125 4.6 x 250 4.6 x 150
5 5 5 5 5 3 3 3 3 5 5 3 5 5 3 5 5 5 5 5 5 5 5 3 3 5 5 3 5
Hypersil BDS-C18 cartridge Hypersil BDS-C18 cartridge Hypersil BDS-C18 cartridge Hypersil BDS-C18 cartridge Hypersil BDS-C18 cartridge Hypersil BDS-C18 cartridge Hypersil BDS-C18 cartridge Hypersil BDS-C18 cartridge Hypersil BDS-C18 cartridge Hypersil BDS-C18 cartridge Hypersil BDS-C8 cartridge Hypersil BDS-C8 cartridge Hypersil BDS-C8 cartridge Hypersil BDS-C8 cartridge Hypersil BDS-C8 cartridge Hypersil ODS cartridge Hypersil ODS cartridge Hypersil ODS cartridge w/CIM Hypersil ODS cartridge Hypersil ODS cartridge w/CIM Hypersil ODS cartridge Hypersil ODS cartridge Hypersil ODS cartridge Hypersil ODS cartridge Hypersil ODS cartridge Hypersil ODS cartridge Hypersil ODS cartridge starter kit (150 mm, 250 mm) Agilent cartridge holder
79926BD-585 79926BD-595 79926OB-584 79926OB-564 79926OB-354 79926BD-344 79926BD-363 79926BD-582 79926BD-562 79926BD-362 79926B8-584 79926B8-564 79926B8-344 79926B8-582 79926B8-562 7992618-585 7992618-595 7982618-584 79926OD-584 7982618-564 79926OD-564 79926OD-354 7992618-363 7992618-582 7992618-562 7992618-362 79926KT-100 5021-1845
Hypersil BDS-C18 cartridge w/CIM 79826BD-584
Hypersil BDS-C18 cartridge w/CIM 79826BD-354
LC and LC/MS
4.0 x 250 4.0 x 250 4.0 x 125 4.0 x 125 4.0 x 100 3.0 x 125 2.0 x 250 2.0 x 125 2.0 x 125 4.0
4.0 x 4 4.0 x 4
5 5
Hypersil BDS 4 x 4 mm Guards 10/pk 79926BD-504 Hypersil ODS 4 x 4 mm Guards 10/pk 7992618-504
*Configurations not shown are available upon request.
566

Hypersil Standard Column
Size (mm) Particle Size (m) Stationary Phase Part No.
4.6 x 250 4.6 x 100 4.6 x 200 4.6 x 100 2.1 x 200 2.1 x 100 4.6 x 250 4.6 x 200 4.6 x 100 4.6 x 100 4.6 x 60 2.1 x 250 2.1 x 200 2.1 x 100 2.1 x 100 2.1 x 100
5 3 5 5 5 5 5 5 5 5 3 5 5 5 5 3
Hypersil BDS-C18 Hypersil BDS-C18 Hypersil MOS Hypersil MOS Hypersil MOS Hypersil MOS Hypersil ODS Hypersil ODS Hypersil ODS Hypersil ODS w/CIM Hypersil ODS Hypersil ODS Hypersil ODS Hypersil ODS Hypersil ODS w/CIM Hypersil ODS
79916BD-584 79916BD-354 79916MO-574 79916MO-554 79916MO-572 79916MO-552 7991618-584 79916OD-574 79916OD-554 7981618-554 79916OD-344 79916PT-582 79916OD-572 79916OD-552 7981618-552 79916OD-352
Hypersil Guard Column

Icon Size (mm) Particle Size (m) Stationary Phase Part No.
LC and LC/MS
4.0 x 20 2.1 x 20 4.0 x 20 2.1 x 20
5 5 5 5
Hypersil MOS Guard Cartridge 3/pk 79916KT-121 Hypersil MOS Guard Cartridge 3/pk 79916KT-111 Hypersil ODS Guard Cartridge 3/pk Hypersil ODS Guard Cartridge 3/pk Guard Cartridge Hardware 79916KT-120 79916KT-110 79900CH-010
Hypersil Capillary Column

Size (mm) Particle Size (m) Stationary Phase Part No.
0.5 x 250 0.5 x 150 0.5 x 35 0.5 x 150 0.5 x 35 0.3 x 250 0.3 x 150 0.3 x 35 0.3 x 150
5 5 5 3 3 5 5 5 3
Hypersil ODS Hypersil ODS Hypersil ODS Hypersil ODS Hypersil ODS Hypersil ODS Hypersil ODS Hypersil ODS Hypersil ODS
5064-8278 5064-8289 5064-8282 5064-8275 5064-8299 5064-8276 5064-8290 5064-8279 5064-8277
567

LiChrospher Reversed-Phase Columns
LiChrospher Reversed-Phase HPLC Cartridge Columns

LiChrospher silica columns are made with spherical, sil-gel type porous silica particles. LiChrospher 60 RP-select B starts with 60 pore size silica optimized for symmetrical peak shapes of basic compounds. LiChrospher 100 is offered in both C8 and C18 as well as an endcapped C18. These columns are noted for high sample capacity and efficiency. The popular LiChrospher packings are offered in the convenient and economical Agilent cartridge configuration. Applications Pharmaceuticals, aromatics Availability 5 m particle size, 60 RP-Select B, 100 RP-18, RP-18 endcapped, RP-8, CN Note: All LiChrospher columns are in Agilent Technologies cartridge design and require reusable cartridge hardware, P/N 5021-1845.
LiChrospher Column
Icon Description Size (mm) Particle Size(m) RP-18 Stationary Phase RP-18 Endcapped RP-8 RP-Select B CN
Cartridge Column Cartridge Column Cartridge Column Cartridge Column Cartridge Column Cartridge Column Agilent cartridge holder
3.0 x 125 3.0 x 250 4.0 x 125 4.0 x 125 4.0 x 250 4.0 x 250
5.0 5.0 5.0 5.0 5.0 5.0 5.0
7992518-563 7992518-583 79925ODE-564* 79925OD-564-3* 79925OD-584 79925OD-58K* 79925OD-504 5021-1845 5021-1845 79925ODE-584 79925OE-58K* 79925MO-584 79925MO-58K* 79925MO-504 5021-1845
79925SB-563 79925SB-583 79925SB-564 79925MO-564-3* 79925SB-56K* 79925SB-584 79925SB-58K* 79925SB-504 5021-1845 79925CN-504 5021-1845
* 3/pk
79925CN-564 79925CN-584
LC and LC/MS
Guard cartridge, 10/pk 4.0 x 4.0
568

LiChrosorb/Nucleosil Reversed-Phase Columns
LiChrosorb Reversed-Phase HPLC Columns

LiChrosorb is a widely used silica packing material manufactured by E. Merck. The irregularly shaped porous silica particles are made by grinding and sifting coarse silica particles into narrow particle size ranges. Agilent offers LiChrosorb columns in C8 and C18 phases and in both 5 and 10 m particle sizes. These standard fitting columns are packed by Agilent using ISOregulated, automated packing procedures to ensure high column-to-column reproducibility. Applications Reversed phase chromatography Support Particle Granular, irregular shaped silica micro-particles Availability 5 and 10 m particle size, 100 pore size
LiChrosorb Column
Size (mm) Particle Size (m) RP-18 Stationary Phase RP-8
4.6 x 200 4.6 x 200
5.0 10.0
79915OD-574 79915OD-174
79915MO-574 79915MO-174
Nucleosil Reversed-Phase HPLC Cartridge Columns

Nucleosil is a spherical porous sil-gel type silica packing material from Machery/Nagel. Agilent packs Nucleosil columns using tight ISO-regulated production control procedures to guarantee consistent quality. Nucleosil 100-5, C18 is used for non-polar to moderately polar compounds in reversed-phase applications. Applications Fatty acids, glycerides, fat soluble vitamins, antibiotics Availability 5 m particle size, 100 pore size
Note: Cartridge columns require reusable cartridge hardware, P/N 5021-1845
LC and LC/MS
Nucleosil Cartridge Column

Icon Description Size (mm) Particle Size (m) Stationary Phase 100-5 C18
Cartridge column Cartridge column Guard cartridge, 10/pk Agilent cartridge holder
4.0 x 125 4.0 x 250 4.0 x 4.0
5.0 5.0 5.0
7992718-564 7992718-584 7992718-504 5021-1845
569

Asahipak/Purospher Reversed-Phase Columns
Asahipak Reversed-Phase HPLC Cartridge Columns

Asahipak ODP-50 is a microparticulate, macroporous polyvinylalcohol based polymeric packing. Designed for analysis of basic substances, Asahipak ODP-50 columns provide high separation efficiency with buffered and alkaline solutions at high pH. These polymeric columns can be used up to pH 13 with a variety of solvents with minimal mechanical swelling or shrinkage. Asahipak ODP-50 is offered in Agilent's convenient cartridge column configuration. Applications Basic compounds Availability 5 m particle size, 80 pore size
Asahipak
Icon Description Size (mm) Particle Size (m) Stationary Phase ODP-50
Cartridge column Cartridge column Cartridge column Agilent cartridge holder Bulk packing
4.0 x 250 4.0 x 125 2.0 x 125 1 gram
5.0 5.0 5.0 5.0
79923OP-584 79923OP-564 7992318-562 5021-1845 79903-85037
LC and LC/MS
Purospher Reversed-Phase HPLC Cartridge Columns

An ultrapure spherical silica support, deactivated by an efficient bonding process, makes Purospher an excellent packing for basic compounds. Multi-step bonding and deactivating processes provide high surface coverage, blocking residual silanol groups and reducing peak tailing. Applications Basic compounds, metal-sensitive compounds Availability 5 m particle size, 80 pore size
Purospher
Icon Description Unit Size (mm) Particle Stationary Phase Size (m) RP-18 RP-18 Endcapped
Cartridge column Cartridge column Guard cartridge
4.0 x 125 4.0 x 250 10/pk 4.0 x 4.0
5.0 5.0 5.0
79925PU-564 79925PE-564 79925PU-584 79925PE-584 79925PU-504 79925PE-504 5021-1845 5021-1845
Agilent cartridge holder
570

Superspher Reversed-Phase Columns
Superspher Reversed-Phase HPLC Columns

Superspher is a high performance 4 m silica support. It offers excellent pressure/ performance ratio for analysis of complex mixtures demanding high peak capacity. Applications Reversed-phase chromatography Availability 4 m particle size, 60 RP-select B, 100 RP-18 and RP-18 endcapped Helpful Hint: At pH 6-8, highest column stability for all silica-based columns is obtained by operating at temperatures <40C using low buffer concentrations in the range of 0.01-0.02 M
Superspher Column
Icon Description Unit Size (mm) Particle Size (m) RP-Select B Stationary Phase RP-18 RP18 Endcapped
Cartridge column Cartridge column Cartridge column Cartridge column Guard cartridge 3/pk Agilent cartridge holder
4.0 x 250 4.0 x 125 2.0 x 250 2.0 x 125 2.0 x 10
4.0 4.0 4.0 4.0 4.0
79925SB-484 79925SB-464 79925SB-482 79925SB-462 79925SB-402 5021-1845
7992518-484 79925OD-464 7992518-482 7992518-462 7992518-402 5021-1845 5021-1845 79925EC-482 79925EC-462
LC and LC/MS
571

SynChropak/Vydac Reversed-Phase Columns
Additional Reversed-Phase Columns for BioScience

Agilent has a selection of C18, C8, and C4 bonded phase spherical silica columns with 300 pore size from other manufacturers. These standard fitting columns are for reversed phase analysis of proteins and peptides.
SynChropak
SynChropak 300 Column
Size (mm) 4.6 x 250 4.6 x 100 Particle Size (m) Pore Size 6.5 6.5 300 300 C4 Stationary Phase C8 C18
79919B3-584 79919M3-584 79919O3-584 79919B3-554 79919M3-554 79919O3-554
Vydac
Vydac 300 Column
Size (mm) 10.0 x 250 4.6 x 250 4.6 x 150 2.1 x 250 1.0 x 250 Particle Size (m) 5.0 5.0 5.0 5.0 5.0 Pore Size 300 300 300 300 300 Stationary Phase C4 (214 TP) C18 (218 TP) 79918B3-588 79918B3-584 79918B3-564 79918O3-588 79918O3-584 79918O3-564 79918O3-582 79918O3-581
LC and LC/MS
572

ZORBAX Normal Phase Columns
Normal Phase Columns ZORBAX Normal Phase HPLC Columns

For normal-phase chromatography, the ZORBAX product line offers a choice of bonded and non-bonded silica packings.
ZORBAX Rx-SIL
Made from highly pure (>99.995%) porous silica microspheres (pore size is the space between the solid silica microparticles) Stronger than other silica types Less acidic than ZORBAX-SIL, lower metal content Low acidity and low metal content make ZORBAX Rx-SIL ideal for normal-phase separation of polar compounds that exhibit poor peak symmetry on more acidic silica Useful for very hydrophilic compounds with high organic mobile phases in reversed phase mode
ZORBAX CN
Cyanopropyldimethylsilane monolayer bonded to ZORBAX SIL Equilibrates more rapidly than ZORBAX SIL, and is used for many of the same normal-phase applications Less prone to fouling and less water sensitive than silica
ZORBAX NH2
Amino-propyl silane phase bonded to ZORBAX SIL Used for normal phase and weak anion-exchange, and reversed-phase HPLC of polar compounds Vitamins A and D are separated in the normal-phase mode Carbohydrates and sugars are separated in the reversed-phase mode on ZORBAX NH2
LC and LC/MS
Specifications
Bonded Phase ZORBAX Rx-SIL ZORBAX SIL ZORBAX CN ZORBAX NH2 Pore Size 80 70 70 70 Surface Area 180 300 m2/g m2/g Carbon Load 7% 4%
300 m2/g 300 m2/g
573

ZORBAX Normal Phase/LiChrospher Columns
High Resolution Normal Phase Separation of Octylphenoxy (polyethylene oxy) Ethanol Surfactant (n= 10) on ZORBAX CN
Operating Conditions Column: ZORBAX CN, 4.6 x 250 mm P/N 880952-705 Mobile Phase Primary: Heptane Secondary: 2-Methoxyethanol/Isopropanol (50/50) Program: 2-20% Secondary in 10 min., Linear Hold at 20% Flow Rate: 2 mL/min Temperature: 50C Detector: 278 nm
0 5 10 Time (min) 15 20
ZORBAX Normal Phase Column*

Icon Column Description Preparative Semi-Preparative Analytical Analytical Analytical Analytical Narrow Bore Guard Cartridges Guard Cartridges Guard Cartridges Guard Hardware Kit Guard Hardware Kit Guard Hardware Kit 2/pk 4/pk 4/pk Unit Size (mm) 21.2 x 250 9.4 x 250 4.6 x 250 4.6 x 250 4.6 x 150 4.6 x 150 2.1 x 150 9.4 x 15 4.6 x 12.5 2.1 x 12.5 9.4 x 15 4.6 x 12.5 2.1 x 12.5 Particle Rx-SIL Size (m) USP L3 7 5 5 5 5 5 5 7 5 5 883700-901 820675-119 820675-119 820675-111 820675-111 820950-919 820950-901 820950-905 820950-908 820950-908 821125-919 840140-901 840140-901 840140-901 840140-901 820777-901 820777-901 820777-901 820777-901 820777-901 820777-901 820777-901 820777-901 820777-901
*Configurations not shown are available upon request. Rx-SIL serves as guard for Rx-SIL and SIL columns. CN serves as guard for CN and NH2 columns.
SIL USP L3
CN USP L10
NH2
Carbohydrate Analysis
880975-101 880952-101 880952-105 880952-108 880975-201 880952-201 880952-205 880952-208 880975-901 880952-701 883975-901 883952-701 883952-705 883952-708 843300-908 880952-705 880952-708 840300-908
LC and LC/MS
Note: See page 563 for "reversed phase" applications using ZORBAX Rx-SIL.
574

Hypersil Normal-Phase/LiChrospher Columns
Hypersil Silica Normal-Phase HPLC Columns

Hypersil silica columns from Agilent combine a strong, high-purity silica and Agilent's superior packing processes. The strength and reproducibility of these silica particles are also available in an amino phase, Hypersil APS (NH2), for normal phase chromatography.
Hypersil Normal-Phase Columns*

Icon Description Unit Narrow Bore Narrow Bore Narrow Bore Analytical Analytical Analytical Analytical Guard cartridge 3/pk Guard cartridge 3/pk Guard cartridge 3/pk Guard cartridge 3/pk Guard hardware kit Particle Size (mm) Size (m) 2.1 x 100 5.0 2.1 x 100 5.0 2.1 x 200 5.0 4.6 x 100 5.0 4.6 x 100 5.0 4.6 x 200 5.0 4.6 x 200 5.0 2.1 x 20 5.0 2.1 x 20 5.0 4.0 x 20 5.0 4.0 x 20 5.0 Stationary Phase Hypersil APS Hypersil SIL Hypersil SIL Hypersil APS Hypersil SIL Hypersil APS Hypersil SIL Hypersil APS Hypersil SIL Hypersil APS Hypersil SIL Part No. 79916AP-552 79916SI-552 79916SI-572 79916AP-554 79916SI-554 79916AP-574 79916SI-574 79916KT-112 79916KT-113 79916KT-122 79916KT-123 79900CH-010
* Configurations not shown are available upon request
Note: Guard cartridges require hardware kits.
LiChrospher Normal-Phase HPLC Cartridge Columns

For normal phase chromatography, Agilent supplies polar modified silica gel phases LiChrospher Si, LiChrospher NH2, LiChrospher DIOL and LiChrospher CN as convenient cartridge columns. LiChrospher Si: Spherical silica for acidic, neutral and basic compounds Great batch-to-batch reproducibility LiChrospher CN, Diol, NH2 Polar modified silica gel phases have both polar and hydrophobic properties Applicable for reversed-phase and normal-phase separations
LC and LC/MS
LiChrospher Column
Icon Description Unit Size (mm) Particle Size (m) Si Stationary Phase CN Diol NH2
Cartridge column Cartridge column Guard cartridge 10/pk Agilent cartridge holder
4.0 x 250 4.0 x 125 4.0 x 4.0
5.0 5.0 5.0
79925SI-584 79925SI-564 79925SI-504 5021-1845
79925CN-584 79925CN-564 79925CN-504 5021-1845
79925DI-584 79925DI-564 5021-1845
79925AP-584 79925AP-564 79925AP-504 5021-1845
575

Ultron Chiral Columns
Ultron Chiral Columns

Direct racemic separations without derivatization. Separate enantiomers of pharmaceuticals, such as hexobarbital, ibuprofen and profenamine, are complete with ULTRON ES-OVM. ULTRON ES-Pepsin Chiral columns are best suited to separate basic compounds that are difficult to separate by other columns. ES-OVM contains an ovomucoid protein while ES-Pepsin columns have pepsin bonded to 120, 5 m silica particles. Both types of chiral columns are usable with mobile phases such as acetonitrile or ethanol and phosphate buffer. Applications ES-OVM: enantiomers of fluoxetine, chlorpheniramine, cloperastin ES-Pepsin: drugs, peptides, eperisone, melizine pH Range ES-OVM 3-7.5; ES-Pepsin 3-6 Availability 5-m particle size, 120 pore size
*Manufactured by Shinwa Chemical Industries, Ltd., Japan
Ultron ES Chiral Columns

Particle Size (m) 5 10 5 5 5 5 702111610 712111630 83211130 Ultron ES-OVM 722111723 724111653 702111651 702111651A 822111651 822111631A Ultron ES-OVM with Guard Ultron ES-Pepsin Ultron ES-Pepsin with Guard
Size (mm) 10.0 x 150 4.6 x 250 4.6 x 150

0 5 10 15 20 Retention Time (min) 25 30
LC and LC/MS
4.6 x 150 2.0 x 150 4.0 x 10 (Guard)
Courtesy of D. S. Risley and V. S. Sharp of Lilly Research Laboratories, Eli Lilly and Co.
Cl H 2N S O O
H N H C 2H 5 C S O NH O Column: Mobile Phase: Flow Rate: Temperature: Detection: ULTRON ES - OVM, 4.6 x 150 mm 20 mM KH 2PO4 (pH 4.6) 1.0 mL/min 25C UV - 220 nm
Separation of Enantiomers of Fluoxetine (Prozac) ULTRON ES-OVM, 4.6 x 150 mm P/N: 702111651 Mobile Phase: 25:75 (v/v) EtOH / 20 mM KH2PO4, pH 5.5 (adjusted with NaOH) Injection: 10 L, 0.8 mL/min Temperature: Ambient Detector: UV (225 nm) Sample: Mixture Fluoxetine (Prozac) entantiomers Column:
10
20
Time (min)
Separation of Ethiazide (diuretic drug) on ULTRON ES-OVM Column
576

ChiraDex Chiral Columns/Polymer Analysis
ChiraDex Chiral Columns

For routine separation of enantiomers Available as ChiraDex cartridge columns Novel manufacturing process bonds -cyclodextrin to spherical 5 m silica gel by means of a chemical spacer Enantiomeric separations have been achieved with ChiraDex using simple nonchiral solvent systems such as MeOH/water, MeOH/buffer, ACN/TEAA* Applications: Barbiturates, benzothioadiazines, calcium antagonists, PTH-amino acids
ChiraDex Cartridge Column

Icon Description Size (mm) Particle Size (m) Part No.
Cartridge column Agilent cartridge holder
4.0 x 250
5.0
79925CB-584 5021-1845
*TEAA=Triethylammonium acetate
Fast Polymer Analysis

Gel Permeation Chromatography (GPC)also referred to as Size Exclusion Chromatography (SEC)is widely used for characterizing polymers in quality control and research laboratories (see table below). The technique has applications in many areas because of its ease of automation and quality of the results it produces. It provides average molecular weights and molecular weight distributions, which are calculated from the chromatogram. Agilent Technologies is a leading supplier of equipment for GPC-SEC, offering a wide range of supplies: GPC-SEC start-up kits to get you up and running quickly and easily EasyCal Vial calibration standards for rapid and convenient calibration Certified/proven columns for organic and aqueous GPC-SEC
LC and LC/MS
GPC-SEC in quality control

Quality control of PVC, polystyrenes and polycarbonates Monitoring resin quality in paint Testing polyamide-6,6 Measuring synthetic polymers such as polyacrylic acids, polyacrylamides and polystyrenesulfonates Analysis of biopolymers such as dextransulfate, starch and humic acids Quality control of insulin
577

Start-up Kits
GPC-SEC Start-up Kits

At Agilent Technologies, we have developed two GPC-SEC Start-up Kitsone for organic-soluble polymers and one for water-soluble polymersto make your entry into the world of GPCSEC as quick, simple, and trouble-free as possible. The kits contain everything you need to get started: a highly-efficient PLgel column, nine vials of ready-to-use mixtures of polymer standards, three vials of a test sample, and a manual with step-by-step instructions. The manual includes troubleshooting hints in case things should go wrong.
GPC-SEC Start-up Kit
The Organic GPC start-up kit contains:

High performance PLgel Mixed-C column, giving a linear calibration up to at least 3 million Da Three mixtures of ready-to-use EasyCal vial calibration standards, based on narrow molecular weight distribution polystyrene and covering a wide MW range (three vials each) Broad MW polystyrene test sample (three vials) Certificates of analysis for column and calibration standards Instruction manual
The Aqueous GPC start-up kit contains:

High efficiency PL aquagel-OH Mixed column, for water-soluble polymers up to at least 10 million Da Three mixtures of ready-to-use EasyCal vial calibration standards based on narrow molecular weight distribution polyethylene oxide and covering a wide MW range (three vials each) Broad MW dextran test sample (three vials) Certificates of analysis for column and calibration standards Instruction manual
GPC-SEC Start-up Kits
LC and LC/MS
Description Organic GPC-SEC Start-up Kit Includes: PLgel Mixed-C column 7.5 x 300 mm, Standards, Test sample, Manual Aqueous SEC Start-up Kit Includes: PL aquagel-OH Mixed 7.5 x 300 mm, Standards, Test sample, Manual
Part No. 5064-8251
5064-8252
578

GPC-SEC Calibration Standards
Simple, Rapid, Reliable Calibration

The EasyCal Vial calibration standards supplied in both kits are ready to use and do not require any special preparation simply add solvent to the 2 mL vial. EasyCal Vial standards consist of the most widely-used polystyrene and polyethylene oxide standards for GPC-SEC calibration. The polymers are specially prepared to ensure an extremely narrow molecular weight distribution, providing accurate GPC-SEC calibration. In addition, the polymer molecular weights are extremely well characterized using multiple characterization techniques. All standards come with a certificate to give you the assurance of Agilent quality. The Start-up kit contains three vials with three different EasyCal Vial calibration standards. Each vial contains four carefully-selected molecular weight polymers. Each EasyCal Vial calibration standard covers a different molecular weight range. Naturally, you can use all three standards to cover the entire range from low to highest molecular weight polymers. The calibration standards are ready to use; the only preparation required is to add solvent to the 2 mL vial and place it in your autosampler tray. Now, you can calibrate frequently, perhaps even daily, to ensure the accuracy and precision of your results. Included in each kit is an appropriate test sample to check the effectiveness of your calibration. The kits contain three vials of each standard mixture and test sample, sufficient for several weeks of work. And each standard mixture is available off-the-shelf when you need it.
Polymer Standards
Description Organic Standards Polystyrene EasyCal Vial standards Aqueous Standards Polyethylene oxide EasyCal Vial standards 10 x 3 Vials 5064-8280 10 x 3 Vials 5064-8281 Unit Part No.
LC and LC/MS
579

GPC-SEC Calibration Standards
Organic Standard Chromatograms Aqueous Standard Chromatograms
Standards in clear vial with red cap
Standards in amber vial with red cap
Standards in clear vial with blue cap
Standards in amber vial with blue cap
LC and LC/MS
Standards in clear vial with green cap
Standards in amber vial with green cap
580

Zorbax PSM GPC-SEC Columns
GPC-SEC Applications using ZORBAX PSM

Agilent ZORBAX PSM Size Exclusion Columns can be used for molecular size separations on most synthetic and natural polymers. The ZORBAX PSM columns are packed with small (5 m) porous silica microspheres (PSM) and are available in two versions. The deactivated version has been silanized for use with non-polar to relatively polar polymers in nonaqueous or partially aqueous solvents and is denoted by an S following the column name. The untreated version is for use with both nonaqueous and aqueous mobile phases. These rigid, siliceous PSM packings have high mechanical strength and are not swelled or dissolved by any common organic or aqueous (pH 27) mobile phases. For samples of wide molecular weight distribution, it is useful to couple columns of one or more pore sizes in series, employing Bimodal or Trimodal Kits. Alternatively, Bimodal and Trimodal columns contain an optimized mixed bed in one column.
ZORBAX PSM Columns (6.2 mm x 250 mm (5 m) Columns)

Description PSM 60 PSM 60S PSM 300 PSM 300S PSM 1000 PSM 1000S PSM 3000 Bimodal-S Bimodal Kit Bimodal Kit-S MW Range 5x 5x 3x 1021 1021 1033 x x x 104 104 105 Silanized No Yes No Yes No Yes No Yes No Yes Part No. 880957-801 880957-802 880957-805 880957-806 880957-807 880957-808 880957-809 880957-814 880949-903
3 x 1033 x 105 1 x 1041 x 106 1 x 1041 x 106 1x 5x 5x 1051 1021 1021 x x x 107 106 106
LC and LC/MS
5 x 1021 x 106
880949-904
Column:
ZORBAX PSM, 6.2 x 250 mm, 5 m P/N 880957-802 Mobile Phase: Dimethyl Formamide Flow Rate: 1.9 mL/min Temperature: Ambient Detector: Refractive Index Detector
Polyurethane Resins
C l
Rapid GPC analysis provides measure of performance characteristics of polyurethane resins.
ZORBAX PSM 60S 6 2 250
581

PLgel GPC-SEC Columns
Agilent PLgel Columns for Reliable and Flexible Polymer Analyses

Agilent Technologies offers a wide range of columns for organic and aqueous GPC-SEC analysis. The PLgel columns, for polymer analysis with organic eluents, are based on a highlycrosslinked polystyrene/divinylbenzene matrix, and are available with different particle and pore sizes to cover a wide range of polymer molecular weight distributions in organic eluents (see figure). The organic GPC Start-up Kit contains a PLgel Mixed-C column, which is the column of choice for general purpose MW distribution. This column is recommended for initial polymer screening; if greater resolution is required, simply add another PLgel column. The PLgel Mixed-C column is packed with 5 m particles of different pore sizes and thus enables the separation of polymers over a wide molecular weight range (from about 200 to 3 million). Such wide ranges usually require sets of several columns, typically between 2 to 3 (up to 6). The chromatogram shows the analyses of a broad technical polystyrene: part a) with one PLgel Mixed-C column and part b) with a set of three PLgel Mixed-C columns. Due to the better separation of the column set and the reduced influence of band broadening, the calculated molecular weight averages are in the latter case closer to the values determined with reference methods, e.g. light scattering (see table).
LC and LC/MS
582

Broad Polystyrene with 1 and 3 PLgel Mixed columns
a) b)
Columns:
a) 1 x PLgel Mixed-C, 7.5 x 300 mm, 5 m (P/N 79911GP-MXC) b) 3 x PLgel Mixed-C, 7.5 x 300 mm, 5 m in series Mobile phase: Tetrahydrofuran (THF) Column temp.: 25C Flow rate: 1 mL/min Detector: Refractive Index Detector Calibrant: Polystyrene EasyCal standards in vials for calibration
LC and LC/MS
Molecular Weight Averages Determined by Reference Methods

Reference Data Difference [%] (1 x PLgel mixed) Difference [%] (3 x PLgel mixed) Mn* 86000 9 3.1 Mw** 246000 15 4.8 D 2.86 27 2.0
* measured by GPC ** measured by light scattering
583

PLgel Columns
ID (mm) Length (mm) Particle Size (m) Pore Size () WeightRange
Polystyrene: Calibration Curves for PLgel Columns

Part No.
PLgel Calibration Curves Calibrants: Polystyrene Eluent: THF Flow rate: 1.0 mL/min.
7.5
300
Polystyrene Molecular Weight
50 100 500 1,000 10,000 100,000 50 <4,000 500 1,000 10,000 100,000 1,000,000
<1,000 <4,000 500-20K 1K-40K 4K-400K 40K-4M <1,000 79911GP-101 500-20K 1K-40K 4K-400K 40K-4M 400K-40M
79911GP-500 79911GP-501 79911GP-502 79911GP-503 79911GP-504 79911GP-505 79911GP-100 79911GP-102 79911GP-103 79911GP-104 79911GP-105 79911GP-106
10M
106
1M 104 100K 103 10K 500 100 1K 50
105
7.5
300
10 100
100 4 5 6 7 Elution Volume (mL) 8 9 10
PLgel Mixed Bed Columns

ID (mm) Length (mm) Particle Size (m)
10M
20 m MIXED - A 10 m MIXED - B 5 m MIXED - C 5 m MIXED - D 3 m MIXED - E
Pore Size ()
WeightRange
Part No.
Polystyrene Molecular Weight
1M
7.5
300
3 5 5 10 20
Mixed-E Mixed-D Mixed-C Mixed-B Mixed-A
up to 30K 200-400K 200-3M 500-10M 1,000-40M
79911GP-MXE 79911GP-MXD 79911GP-MXC 79911GP-MXB 79911GP-MXA
100K
10K
LC and LC/MS
PLgel Guard Columns

1K
100
Part No.
7 8 Elution Volume (mL)
10
7.5 7.5
50 50
5 10
79911GP-510 79911GP-110
584

ZORBAX GF-250/GF-450 GFC Columns
ZORBAX GF-250 and GF-450 Gel Filtration Columns

Rapid separations and extended column life timeoften twice that of other size exclusion columns. High recovery for most proteins (>90%) Usable with organic modifiers and denaturants Silica surface is stabilized with zirconia, then coated with a monolayer diol phase providing a broad pH range of 3-8.5a highly reproducible, hydrophilic column. ZORBAX SIL particles provide a silica support with high physical stability For an in-depth discussion of the GF-250 and GF-450 columns, request publication number 5966-2063E from our website or from your local Agilent office or distributor.
ZORBAX GF-250 and GF-450 Column Specifications

Bonded Phase Pore Particle Size Size MW Range Surface Area pH Flow Range Rate Max Pressure
ZORBAX GF-250 150 ZORBAX GF-450 300
4 m 6 m
4,000-400,000 10,000-900,000
140 m2/g 3.0-8.0 <3.0 mL/min 5000 psi 50 m2/g 3.0-8.0 <3.0 mL/min 5000 psi
ZORBAX 300Extend-C18 Column

Column Description Unit Size (mm) Particle Size (m) Part Number
GF-250, 150 pore size GF-250, 150 pore size GF-250, 150 pore size GF-450, 300 pore size GF-450, 300 pore size GF-250 Guard cartridge GF-250 Guard cartridge GF-450 Guard cartridge GF-450 Guard cartridge GF-250, GF-450 Hardware kit GF-250, GF-450 Hardware kit 2/pk 4/pk 2/pk 4/pk
21.2 x 250 9.4 x 250 4.6 x 250 21.2 x 250 9.4 x 250 9.4 x 15 4.6 x 12.5 9.4 x 15 4.6 x 12.5 9.4 x 15 4.6 x 12.5
4 4 4 6 6 4 4 4 4
884974-901 884973-901 884973-701
LC and LC/MS
884974-910 884973-902 820675-111 820950-911 820675-111 820950-911 840140-901 820777-901
585

GF-450 GFC Columns
High-Speed Size Exclusion Separations
A: BSA and BSA Dimers (duplicate injections) 1. BSA dimer 2. BSA monomer 3. Azide Conditions: Column: Flow Rate: Eluent: 2 Temperature: Detector: B: Antibody Separation
Conditions: Column: 2 Mobile Phase:
ZORBAX GF-450 9.4 x 250 mm 3 mL/min PBS (phosphate buffered saline), pH 7.4 Ambient 2 UV, 220 nm
1. IgG Dimer and Aggregate 2. Mouse IgG 1K (MOPC 31C) 3. Salts: Tris, Na2HPO4, NaCl
Flow Rate: Detector: Sample:
ZORBAX GF-450 9.4 x 250 mm 200 mM Na Phosphate Monobasic pH 7.0/0.1% Azide 2.0 mL/min UV - 225 nm 10 g in 50 mM Sodium Phosphate pH 7.0
3 1 1 Inj. #1 3 Inj. #2 1 3 0 1 2 3 4 5 6 7 8 9 10
Retention Time (min)
5 Time (min)
10

Save time and avoid trial and error by accessing pre-defined, high-quality applications.
LC and LC/MS
Consult Agilent's online, searchable Chromatogram Database

586
LC and LC/MS
ZORBAX GF-250 Columns
Separation of a Protein Mixture on the 9.4 x 250 mm ZORBAX GF-250 Column
1. 2. 3. 4. 5. 6. 7. Mouse IgM 900,000 Da Bovine Thyroglobulin 670,000 Da Sweet Potato - Amylase 200,000 Da Bovine Serum Albumin 67,000 Da Chicken Albumin 45,000 Da Bovine RNAse 13,700 Da Azide 65 Da 5 4 3 1 6 7 Flow Rate: Eluent: Detection: 1 mL/min 130 mM NaCl/20 mM KCl/ 50 mM Na2HPO4, pH 7.0 UV - 210 nm
10
12
14
Time (min)
ZORBAX GF-250 Shows Extended Column Lifetime

Stability of GF-250: K* of Standard Proteins 3.0 2.5 2.0 K* 1.5 1.0 0.5 0.0 0 1000 2000 Column Volumes 3000 BSA K* Ovalbumin Lysozyme NaN3 1.0 0.5 0.0 0 1000 2000 Column Volumes 3000 Stability of Competitor Column: K* of Standard Proteins 3.0 2.5 2.0 1.5 BSA Ovalbumin Lysozyme NaN3
LC and LC/MS
587

PL aquagel-OH SEC Columns
PL aquagel-OH Columns for Reliable and Flexible Polymer Analyses

The PL aquagel-OH mixed column, with its extremely hydrophilic polyhydroxyl surface, is suitable for the aqueous SEC analysis of water-soluble polymers over a wide range of molecular weights (see figure). The Agilent PL aquagel-OH column can handle most neutral hydrophilic polymers, and its capability extends to the analysis of high molecular weight polymers including polyacrylamides and polyethylene oxides. The aqueous SEC Start-up kit contains a PL aquagel-OH mixed column, with 8 m particles, which is the column of choice for MW distributions in aqueous eluents. This column is recommended for first polymer screening; if greater resolution is required, simply add another PL aquagel-OH column.
PL aquagel-OH Columns
ID (mm) Length (mm) Particle Size (m) Pore Size () WeightRange
Part No.
7.5
300
30 40 50 60
100-30K 10K-200K 50K-1M 200K-10M 100-10M
79911GF-083 79911GF-084 79911GF-085 79911GF-086 79911GF-MXA
7.5
300
Mixed
PL aquagel-OH Guard Columns

LC and LC/MS
Part No.
7.5
50
79911GF-080
PL aquagel-OH Resolving Ranges (PEO)
588

PL aquagel Columns
Dextrans are widely used in products in the pharmaceutical, cosmetics, paint, adhesives, and paper industries. Their molecular weight averages can vary from 3 x 104 to 3 x 107 daltons. Their properties depend strongly on molecular weight and the width and shape of the molecular weight distribution. Aqueous SEC is an excellent technique for monitoring product quality. The dextran used in cosmetic formulations was analyzed first using a single PL aquagel-OH Mixed column. In the upper chromatogram, a very small shoulder on the front of the peak suggests the possible presence of a coeluting impurity. The addition of a single PL aquagelOH 30 column provided additional resolution (lower chromatogram), which clearly reveals the impurities. Column: PL aquagel-OH Mixed, 7.5 x 300 mm, 8 m P/N 79911GF-MXA Mobile phase: Water Column temp.: 25C Flow rate: 1 mL/min Detector: Refractive Index Detector Calibrant: Polyethylene oxide EasyCal standards in vials for calibration PL aquagel-OH Mixed, 7.5 x 300 mm, 8 m P/N 79911GF-MXA in series with PL aquagel-OH 30, 7.5 x 300 mm, 8 m P/N 79911GF-083 Mobile phase: Water Column temp.: 25C Flow rate: 1 mL/min Detector: Refractive Index Detector Calibrant: Polyethylene oxide EasyCal standards in vials for calibration Columns:
LC and LC/MS
589

ZORBAX Ion Exchange Columns
ZORBAX SAX, SCX

ZORBAX ion exchange columns (70 SAX and 300 SCX) use ZORBAX SIL porous silica microspheres as the stationary phase support; ion exchange groups are attached to a diol backbone. - SCX uses aromatic sulfonic acid - SAX uses a trifunctional quaternary amine Provides highly efficient, rapid separations (pressure limit 5,000 psi) Applications SAX aromatic carboxylic acids, aliphatic carboxylic acids, and sulfonic acids SCX basic water soluble compounds Availability 5 m particle size, pore sizes: 70 (SAX), 300 (SCX)
ZORBAX SAX and SCX Column Specifications

Bonded Phase ZORBAX SCX ZORBAX SAX Cough-cold RemediesZORBAX SCX
ZORBAX SCX, 4.6 x 250 mm Mobile Phase: 0.1 M NaH2PO4 (pH 6.5) Flow Rate: 3.0 cm3/min Temperature: 40C Detection: UV, 210 nm Peak Identity 1. Pyrilamine 2. Theophylline 3. Glyceryl Guaicolate 4. Caffeine 5. Phenylephrine Conditions Column:
Pore Size 300 70
Surface Area 50 m2/g 300m2/g
pH Range 2.0-7.0 2.0-7.0
Bonded Functionality Sulfonic acid Quaternary amine
ZORBAX SAX and SCX Column

Size (mm) 9.4 x 250 4.6 x 250 4.6 x 150 2.1 x 150 4.6 x 50 3.0 x 50 2.1 x 50 4/pk 4.6 x 12.5 Particle Size (m) 5 5 5 5 5 5 5 5 820950-903 820777-901
Description Semi-preparative Analytical Analytical Analytical Analytical Analytical Analytical Guard cartridge
Unit
SAX (70) 880952-203 880952-703 883952-703
SCX (300) 880952-204 880952-704 883952-704 883700-704 846952-704 860700-304 860700-704 820950-904 820777-901
LC and LC/MS
Reusable guard cartridge hardware
590

Other Ion Exchange/HIC Columns
PL and SynChropak Ion Exchange Columns

For your convenience, Agilent provides a selection of silica and polymeric ion-exchange columns from other manufacturers.
Anion Exchange Column

Particle Size Pore pH Size (mm) (m) Size () Range PL 1000 SCX Stationary Phase SynChropak SynChropak WAX SAX
4.6 x 100 4.6 x 250 5.0 x 50
6 6 8
300 300
2-8 2-8 1-13
79919DE-754 79919QA-754 79919DE-784 79919QA-784 79931QA-835
Cation Exchange Column

Particle Size Pore pH Size (mm) (m) Size () Range Stationary Phase SynChropak SynChropak WCX SCX
4.6 x 100 4.6 x 250
6 6
300 300
2-8 2-8
79919CM-754 79919SP-754 79919CM-784 79919SP-784
SynChropak Hydrophobic Interaction Columns

Hydrophobic interaction chromatography (HIC) offers different selectivity than ion exchange or reverse phase LC for the separation of proteins. Used for purification as well as a primary analytical ion exchange No organic solvents are used and proteins are not denatured Often used as a complement to ion-exchange or size exclusion chromatography
LC and LC/MS
Hydrophobic Interaction Column

Size (mm) Particle Size Pore pH (m) Size () Range Stationary Phase SynChropak H-Propyl
4.6 x 100
300
2-8
79919P3-754
591

Special Applications Columns and Kits
Separation of Carbohydrates in Syrups
Glucose Fructose Maltose Corn Syrup 1 : 50 dilution Raffinose Pancake Syrup 1 : 100 dilution Glucose Sucrose Maltose Fructose Raffinose
ZORBAX Carbohydrate Analysis Columns

Reproducible-each lot of this applications specific aminopropyl column packing material is use-tested for specific monosaccharide and disaccharide separations Efficient-uses ZORBAX porous silica microsphere technology. Silica manufacturing, bonding and packing are all performed in our ISO9001 facilities Flexible-can handle high volume injectionsas much as 50 L on a 4.6 x 150 mm column.
Column: ZORBAX Carbohydrate Analysis Column, 4.6 x 150 mm 75% ACN/25% H2O Agilent 1100 RID 30C 2.0 mL/min
Fructose Glucose
Honey 1 : 200 dilution
Sucrose Fructose Glucose
Molasses 1 : 50 dilution
Mobile Phase: Instrument: Temperature: Flow Rate:
Glucose Fructose 0 1 2 3 4
Caramel Syrup 1 : 10 dilution
min
ZORBAX Carbohydrate Analysis Column Specifications

Bonded Phase ZORBAX carbohydrate column Pore Size 70 Surface Area 300 m2/g pH Range 2.0-8.0 Endcapped No Carbon Load 3.5%
ZORBAX Carbohydrate Analysis Column*

Column Description Unit Size (mm) Particle Size (m) Part No.
LC and LC/MS
ZORBAX carbohydrate analysis column ZORBAX carbohydrate analysis column ZORBAX NH2 guard Guard cartridge hardware kit 4/pk
4.6 x 250 4.6 x 150 4.6 x 12.5
5.0 5.0 5.0
840300-908 843300-908 820950-908 820777-901
*Other configurations are available by special products quote
Separation of Simple-Sugar and Sugar-Alcohol Standards

Fructose Xylose Xylitol Rhamnose Sucrose Maltose Lactulose Lactose Raffinose Xylose Rhamnose Glu/Gal
Rhamnose, Xylose, Xylitol, Lactulose, Raffinose (54 g each) Fructose (10 g), Glucose, Sucrose (36 g each) Maltose, Lactose (6 g each), Inj. = 6.3 L Rhamnose, Xylose, Raffinose (54 g each), Fructose (10 g) Glucose, Sucrose (36 g each), Maltose, Lactose (60 g each) Inj. = 6.3 L Sample: (54 g each), Inj. = 6.3 L
min
Sucrose Maltose Lactose Glucose Fructose
Raffinose
Xylose Rhamnose Xylitol Mannitol 1 2 3 4 5 6 7 Lactulose Raffinose
ZORBAX Carbohydrate Analysis Column, 4.6 x 150 mm Mobile Phase: 75% ACN/25% H2O Flow: 2 mL/min Temperature: 30C Detection: Agilent 1100 RID Det. Temp: 30C
Column:
592

HPLC Columns for DNA Separations

ZORBAX Eclipse dsDNA
Optimized for HPLC separation of dsDNA fragments in the 20-1500 bp range High capacity and sensitivity - Up to 10 g can be loaded onto a 2.1 x 75 mm column, up to 40 g onto a 4.6 x 75 mm column - Can detect 1 ng peak High resolutionas little as 1 bp depending on time and fragment size Useful for several applications: - PCR fragments - DHPLC - RAPD - Restriction digests - RFLP HPLC advantages over conventional gel electrophoresis: - Simple, quantitative analysis - Automated, unattended runs and less hands-on time - Easy fraction collection - Easy preparation and recovery of DNA without tedious gel recovery proceduressimply lyophilize - Direct fraction collection - Sensitive direct detection by UV or fluorescence
mAU 30 25 20 15 10 5 0 0 6772 10 89 20 1353 1078 501 872 353 489 603 242 281 234 271 310 404 194 110 118 147 190 30 40 50 60 70 80 min
High Resolution A.Separation on a 2.1 x 150 mm Column Gradient: 45-75% B in 90 minutes

504 540 458 587 434 213234 192 287 123/124184 80 89 104
mAU 30 20 10 0 0 5 10 15 20 51 57 64
25
min
High Throughput B.Separation on a 4.6 x 75 mm Column Gradient: 45-80% B in 30 minutes Instrument: Column: Agilent 1100, 1 L flow cell, binary pump A: Eclipse dsDNA, 2.1 x 150 mm P/N 993850-902 B: Eclipse dsDNA, 4.6 x 75 mm P/N 992750-902 A. 0.1 M TEAA*, 0.1 mM EDTA, pH 7.0 B: A with 25% acetonitrile A: 0.2 mL/min B: 1.0 mL/min 200 ng restriction digest standards
*TEAA = triethylammonium acetate
Mobile Phase: Flow Rate:
ZORBAX Eclipse dsDNA Column Specifications

Bonded Phase Eclipse dsDNA Pore Size Proprietary Surface Area 12 m2/g pH Range 2.0-9.0
Sample:
LC and LC/MS
ZORBAX Eclipse dsDNA Column*

Description Unit Size (mm) Particle Size (m) Part No.
Analytical Analytical Analytical Preparative Guard cartridge, analytical Guard cartridge, preparative Guard hardware kit 4/pk 4/pk
2.1 x 75 2.1 x 150 3.0 x 75 4.6 x 75 2.1 x 12.5 4.6 x 12.5
3.5 3.5 3.5 3.5 3.5 3.5
945750-902 993850-902 945750-302 992750-902 821125-929 820950-929 820777-901
* Other configurations are available by special products quote
593

Separation of Standard p(DT) 12-18 on ZORBAX Oligo Column
30.46 33.56
ZORBAX Oligo Columns

Mixed mode ion exchange and reversed phase columns specifically developed for oligonucleotide purification. Unique ligand is bonded to 5 m, 150 silica that has been stabilized with zirconium oxide. Denaturants can be used to prevent self-hybridization. Applications Heterodeoxyoligonucleotides, purification of oligonucleotides pH Range 2.5 to 8.5
24.03 27.28
36.59 39.47 42.21
10
15
20
25
30
35
40
45
50
55 Time (min)
Column: ZORBAX Oligo, 6.2 x 80 mm Mobile Phase: Gradient: 35 to 85% B in 90 min. A = 20:80 ACN: 20 mM Sodium Phosphate pH 7.0 B = 20:80 ACN: 20 mM Sodium Phosphate pH 7.0 + 0.8 M NaCl Flow Rate: 1.0 mL/min Temperature: Ambient Detection: UV-260 nm, 2.0 AUFS Sample Volume: 50 L, 5 units/mL
Availability 150 pore available in 5 m particle size
ZORBAX Oligo Column Specifications

Bonded Phase ZORBAX Oligo Pore Size 150 Surface Area 140 m2/g pH Range 2.5-8.5 Bonded Functionality Proprietary diol
ZORBAX Oligo Column

Size (mm) 6.2 x 80 9.4 x 250 21.2 x 250 Particle Size (m) 5.0 5.0 5.0 Stationary Phase Oligo 820940-901 884973-903 884974-915
LC and LC/MS
594

Validation Kits/Custom Column Ordering Procedure
ZORBAX Validation Kits

ZORBAX Validation Kits are supplied to customers who need the same HPLC column type (bonded phase, particle size, configuration) but from different manufacturing lots. To request columns from different lots, contact Agilent Technologies or your local distributor using the following procedure. 1. Request Validation Kits (columns from different lots) by using Part Number 899999-888 2. Indicate the Part Number of the current column you are using 3. Indicate the Lot Number of the current column you are using 4. Indicate the number of additional columns needed from different lots (example: you have a current column and may need two additional lots) 5. Please fax your request to (302) 993-5354 or email to custom_columns@agilent.com. You will receive a quote from your Customer Service Agent within 1-2 business days. Delivery for your custom column is usually 3 weeks or less from the time your order is placed, depending on lot availability.
Custom HPLC Column Ordering

Custom Column Ordering Procedure for ZORBAX HPLC Columns
Columns not listed can easily be ordered by the following procedure: 1. Request a Special Products Quotation (SPQ) using P/N 899999-999 2. Indicate for this part number Column dimensions (example: 4.6 x 50 mm) Bonded phase type (example: StableBond C3) Particle size (example: 5 micron) Pore size (80 or 300)
LC and LC/MS
3. Please fax your request to (302) 993-5354 or email to custom_columns@agilent.com. You will receive a quote from your Customer Service Agent within 1-2 business days. Delivery for your custom column is usually 3 weeks or less from the time your order is placed.
595

HPLC Column Kits

ZORBAX Method Development KitsThree Columns for the Price of Two!!
Agilent offers a series of kits that allows for fast method development at an attractive price. Each kit contains three columns. To study the effect of a change in selectivity on your separation under a given set of conditions, try either the StableBond (see Figure) or Eclipse kits depending on if you are operating at low pH or mid-range pH, respectively. Try the pH kits if you want to study the effect of pH on your separation over a wide range of pH (1-11.5). The aqueous kits provide a wide range of selectivities with a set of columns that can operate under high aqueous conditions to retain highly polar analytes.
Zorbax Method Development Kits

StableBond Method Development Kit: includes 4.6 mm x 150 mm, 5 m columns; one each: SB-C18, SB-CN and SB-Phenyl phases Fast StableBond Method Development Kit: includes 4.6 mm x 75 mm, 3.5 m columns; one each: SB-C18, SB-CN and SB-Phenyl phases Eclipse XDB Method Development Kit: includes 4.6 mm x 150 mm, 5 m columns; one each: XDB-C18, XDB-C8 and XDB-Phenyl phases Fast Eclipse XDB Method Development Kit: includes 4.6 mm x 75 mm, 3.5 m columns; one each: XDB-C18, XDB-C8 and XDB-Phenyl phases New! pH Method Development Kit: includes 4.6 mm x 150 mm, 5 m columns; one each: SB-C18, XDB-C18 and Extend-C18 phases New! Fast pH Method Development Kit: includes 4.6 mm x 75 mm, 3.5 m columns; one each: SB-C18, XDB-C18 and Extend-C18 phases New! Aqueous Method Development Kit: includes 4.6 mm x 150 mm, 5 m columns; one each: SB-Aq, Bonus RP and SB-C18 New! Fast Aqueous Method Development Kit: includes 4.6 mm x 75 mm, 3.5 m columns; one each: SB-Aq, Bonus RP and SB-C18
5183-4624 5183-4625 5183-4626 5183-4627 5185-5807 5185-5808 5185-5809 5185-5810
LC and LC/MS
SB-CN (P/N 883975-905)
Herbicides Examples of Bonded Phase Selectivity Differences

ZORBAX StableBond Columns 4.6 x 150mm, 5m samples: 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. Bentazon Teburthiron Simazine Altrazine Prometon Diuron Propazine Propanil Prometryne Metolachor
SB-Phenyl (P/N 883975-912)
Mobile Phase: 35% ACN, 65% H2O
SB-C8 (P/N 883975-906)
Flow Rate:
1 mL/min
596

Agilent Cartridge System Starter Kits

Agilent Cartridge System Starter Kits
Icon Description ZORBAX C18 Kit: contains one Eclipse XDB-C18 column 4.6 x 150 mm, 5 m; one StableBond C18 column 4.6 x 150 mm, 5 m; cartridge holder, mounting tool, replacement filter (2/pk), wrench, open end ZORBAX C8 Kit: contains one Eclipse XDB-C8 column 4.6 x 150 mm, 5 m; one StableBond C8 column 4.6 x 150 mm, 5 m; cartridge holder, mounting tool, replacement filter (2/pk), wrench, open end Hypersil ODS Kit, Cartridge Starter Kit: Hypersil ODS, 5 m: contains Hypersil ODS, 125 x 4 mm; Hypersil ODS, 250 x 4 mm; cartridge holder, mounting tool, replacement filter (2/pk), wrench, open end Reversed-Phase Method Development Kit: 4 mm id: contains 4 cartridges, 125 x 4 mm, packed with Hypersil ODS, 5 m, Hypersil BDS-C18, 5 m, LiChrospher RP-8, 5 m, and Nucleosil C18, 5 m; 10 guard cartridges, cartridge holder, 10 replacement filters, mounting tool for replacing the filters; PEEK tubing (0.18 mm id), 4 PEEK fittings; LC trouble shooting guide and the supplies catalog on CD-ROM Reversed-Phase Method Development Kit: 2 mm id: contains 4 cartridges, 125 x 2 mm, packed with Hypersil BDS-C18, 5 m, Hypersil BDS-C18, 3 m, Hypersil ODS, 3 m, and Superspher Select B, 4 m; 3 guard cartridges, cartridge holder, 10 replacement filters, mounting tool for replacing the filters; PEEK tubing (0.13 mm id), 4 PEEK fittings; LC troubleshooting guide and the supplies catalog on CD-ROM Part No. 5183-2021
5183-2022
79926KT-100
5063-6516
5063-6529
LC and LC/MS
597

HPLC Special Application Kits and Supplies

Amino Acid Separation Kit for use with 1100*
Icon Description AA kit includes: Hypersil AA (AminoQuant) Column, guard cartridges, guard cartridge holder, connecting capillary, AA standards and reagents, technical note, method disk
Hypersil AA Columns
Part No. 5063-6588
Hypersil AA (AminoQuant) Column for Amino Acid Analysis, 2.1 x 200 mm Guard Columns, Hypersil ODS, 2.1 x 20 mm (3/pk) Guard Cartridge Holder Connecting Capillary 0.12 x 35 mm (2/pk) ZORBAX Eclipse AAA Columns Eclipse AAA, 4.6 x 150 mm, 5 m Eclipse AAA, 4.6 x 150 mm, 3.5 m Eclipse AAA, 4.6 x 75 mm, 3.5 m Eclipse AAA, 3.0 x 150 mm, 3.5 m Guard Cartridges, Eclipse AAA, 4.6 x 12.5 mm, 5 m Guard Cartridge Hardware Kit
79916AA-572 79916KT-110 79900CH-010 79841-87609 993400-902 963400-902 966400-902 961400-302 820950-931 820777-901
LC and LC/MS
Amino Acid Standards Each amino acid standard contains the following amino acids: Glycine L-tyrosine L-serine L-glutamic acid L-arginine L-cystine L-leucine L-alanine L-proline L-threonine L-histidine L-methionine L-phenylalanine L-isoleucine L-valine Description Amino acid standard (1 nmol/L), 10 L 1 mL ampoules Amino acid standard (250 pmol/L), 10 L 1 mL ampoules Amino acid standard (100 pmol/L), 10 L 1 mL ampoules Amino acid standard (25 pmol/L), 10 L 1 mL ampoules Amino acid standard (10 pmol/L), 10 L 1 mL ampoules Amino acids supplement kit: Includes 1 g each of norvaline, sarcosine, asparagine, glutamine, tryptophan, and 4-hydroxyproline
L-lysine L-aspartic acid
Part No. 5061-3330 5061-3331 5061-3332 5061-3333 5061-3334 5062-2478
*Buy limited quantities because of shelf-life considerations.
598

Amino Acid Separations Reagent
Description
Reagents for Amino Acid Analysis
Part No.
OPA reagent, 10 mg/mLeach in 0.4 M borate buffer 5061-3335 o-phthalaldehyde (OPA) and 3-mercaptopropionic acid, 6 ampoules FMOC reagent, 2.5 mg/ml in acetonitrile, 9-fluorenylmethylchloroformate, 1 mL Borate buffer: 100 mL DTDPA (Dithiodiproprionic) reagent for analysis of cysteine, 5 g 5061-3337 5061-3339 5062-2479
Special Columns
Icon Description PTH Column, 2.1 x 250 mm, 5 m
PAH Columns
V V
Part No. 79916PT-582 79918PAH-584 79918PH-534 79918PH-100 79918PAH-582 79918PH-532 79918PH-100 79925PA-583 8500-6035
V V
PAH Column (Vydac), 4.6 x 250 mm, 5 m PAH Guard Column (Vydac), 4.0 x 30 mm, 5 m Guard Column Hardware PAH Column (Vydac), 2.1 x 250 mm, 5 m PAH Guard Column (Vydac), 2.0 x 30 mm, 5 m Guard Column Hardware PAH Column (LiChrospher), 3.0 x 250 mm, 5 m PAH Certified Chemical Standard
Pesticide Column
LC and LC/MS
Hypersil ODS Column, 2.1 x 100 mm, 3 m Certified Pesticide Standard - 3 Ampoules, 1 mL each (Atrazine, Clortouron, Cyanacine, Desethylatrazine, Diuron, Hexazinone Isoproturon, Linuron, Metazachlor, Metabromuron, Metolachlor, Metoxuron, Monolinuron, Sebutylazine, Simazine, Terbutylazine)
79916OD-352 5062-2500
Anion Chromatography Kit

Icon Description Asahipak ODP-50 LC cartridge, 5 m, 4 x 125 mm Cartridge holder Mobile phase additive Check out sample, 10 mL (Fluoride, Chloride, Bromide, Phosphate, Nitrite, Nitrate, Sulfate) Part No. 79923OP-564 5021-1845 5062-2480 5062-8524
599
LC and LC/MS
LC and LC/MS Troubleshooting

HPLC Troubleshooting
Symptom Type Possible Cause Solution Use mobile phase for sample solvent Replace seals/gaskets Flush column, cleanup sample, use pure solvents Use non-absorbing or HPLC-grade solvent Use higher UV wavelength Use non-absorbing or HPLC-grade solvent Insulate column and tubing Thermostat column and tubing Monitor room temperature and control Flush column at end of run with strong solvent Flush column to remove contaminants Sample cleanup or pre-fractionation Prepare sample in actual mobile phase to minimize disturbance Prepare fresh daily; use anti-oxidant Check suitability of water by running different amounts through reversed phase column and measure peak height with elution; use HPLC grade De-gas solvents Better sample cleanup; use guard column Use lower viscosity solvents or higher temperature Use larger dp packing Replace end fitting Reverse solvent flow Tighten fitting, cut tubing, or replace ferrule Replace rotor in valve Tighten or replace fitting Replace pump seal No problem; reverse polarity to make positive Use mobile phase with lower UV absorbance; do not recycle solvent too long Flush column; cleanup sample; use HPLC-grade solvent Replace UV lamp (lasts 1000 hrs) Use voltage stabilizer for LC system De-gas mobile phase or back pressure regulator Baseline disturbance at void time Positive/negativeDifference in refractive index of injection solvent Detector leaks Drifting Baseline CatastrophicFailed detector seal Positive directionContaminant buildup/elution Negative direction (gradient)Absorbance of A mobile phase solvent Positive direction (gradient)Absorbance of mobile phase B Positive direction (gradient)Absorbance of B" mobile phase solvent RandomTemperature changes RandomTemperature changes Wavy or undulatingTemperature changes in room Ghost peaks Peaks from previous injection Contamination Unknown interferences in samples Ion pairUpset equilibrium Peptide mappingOxidation of TFA Reversed phaseContaminated water
LC and LC/MS
SpikesBubbles in solvent High column backpressure Column blockage with irrev. adsorbed sample Too high mobile phase viscosity Particle size too small Plugged inlet frit Plugged inlet frit Leak Leak, injection valve Leak, column or other fittings Leak, pump Negative peaks Subtle; white powder at fittingloose fitting CatastrophicWorn valve rotor CatastrophicLoose fittings CatastrophicPump seal failure RI detectorsolute refractive index less than solvent UV detectorsolute absorbance less than mobile phase Noisy baseline RandomContaminant buildup ContinuousDetector lamp problem OccasionalExternal electrical interference SpikesBubble in detector
600
LC and LC/MS
HPLC Troubleshooting (continued)
Symptom Type Peak doubling Possible Cause Sample volume too large Injection solvent too strong Blocked frit Column void or channeling Unswept injector flowpath Void at head of column Column overloaded with sample Solution Injection volume should be 1/6 when mobile phase used for injection Use weaker injection solvent or mobile phase Replace and use 0.5 l porosity in-line filter Fill in void with glass beads or packing; repack column Replace injector rotor Top off column with packing or glass beads Use higher capacity stationary phase Increase column diameter Decrease sample size Sample cleanup; prefractionation See peak doubling Minimize number of connections Ensure injector seal is tight Ensure fittings are properly seated Switch to polymeric phase Use stronger mobile phase or add competing base (e.g. TMA) Use speciality column; polymeric column, or sterically protected Add salt to mobile phase to increase buffer concentration Lower mobile phase pH to suppress Derivatize solute to change polar interactions Decrease solvent strength of injection solvent to focus solute Introduce air bubble in front/back of sample to decrease dispersion Increase frequency of sampling Adjust time constant to match peak width Increase column temperature Use smallest possible cell volume with no heat exchanger in system Decrease injection volume Use gradient elution or stronger mobile phase Replace check valve De-gas, helium sparg Replace pump seals Filter sample; in-line filter; filter mobile phase Test buffer-organic mixtures; ensure compatibility Add mobile phase modifiers or change solvent
Single peakInterfering components Peak tailing Beginning of peak doubling Unswept dead volumes
Basic compoundsSilanol interaction Basic substancesSilanol interactions Silica-basedColumn degradation Silica-basedSilanol interaction
Peaks are broad
Injection volume too large Peak dispersion in injector valve Sampling rate of data system too slow Slow detector time constant Mobile phase viscosity too high Detector cell volume too large Injector volume too large Long retention times
LC and LC/MS
Pressure fluctuation
Leaky check valve Bubble in pump Pump seal leaks
Pressure increasing
Buildup of particulates Water/organic systemsBuffer precipitation
Retention beyond total permeation volume Size exclusionSpecific interactions with stationary phase
601
LC and LC/MS
HPLC Troubleshooting (continued)
Symptom Type Retention times are changing Possible Cause Column temperature varying Equilibration time insufficient with gradient run or changes in isocratic mobile phase Selective evaporation of mobile phase component Buffer capacity insufficient Inconsistent on-line mobile phase mixing Contamination buildup Retention times are decreasing Flow rate increasing Column overloaded with sample Loss of bonded stationary phase Retention times are increasing Flow rate is slowing Active sites on silica packing Loss of bonded stationary phase Mobile phase composition changing Active sites on silica packing Active sites on silica packing Sensitivity problem Peaks are outside of linear range of detector First few sample injectionsAbsorption of sample in loop or column Autosampler flow lines blocked Injector sample loop under filled Sample-related losses during preparation Slow column equilibration times (ion pairing) Equilibration time slow for long-chain ion pairing reagents Solution Thermostat column; insulate column; ensure lab temp constant Make sure at least 10 column volumes pass through column after solvent change or gradient conclusion Less vigorous helium sparging; keep solvent reservoirs covered; prepare fresh mobile phase Use >20 mM concentration of buffer Ensure gradient system delivering constant composition; check vs. manual prep of mobile phase Flush column occasionally to remove contaminants Check pump to make sure correct; reset if not Decrease sample size Keep mobile phase pH between 2 and 8.5 Fix leaks in liquid lines, replace pump seals, check for pump cavitation or air bubbles Use mobile phase modifier Keep pH of mobile phase in 2 to 8.5 range Make sure mobile phase container is covered Add competing base to mobile phase Use higher coverage packing for stationary phase Dilute/concentrate to bring into linear region Condition loop/column with concentrated sample Check flow and make sure no blockages Make sure that loop is over filled with sample Use internal standard during sample prep; optimize sample prep method Use shorter alkyl chain reagent
First few injectionsAdsorption on active sites Condition column by initial injection of concentrated sample
LC and LC/MS
602
LC and LC/MS
LC/MS Troubleshooting
Symptom No Peaks Solution Spray from the nebulizer. Make sure capillary voltage is set correctly. Make sure LC/MSD is tuned correctly. Make sure LC/MSD pressures are within normal ranges. Check drying gas flow and temperature. Make sure fragmentor is set correctly. Recalibrate the mass axis Make sure ions used for tuning, span mass range of the sample ions and show strong stable signals. Check the solution chemistry. Make sure solvent you're using is appropriate for your sample. Make sure the sample is fresh and has been stored correctly. Make sure LC/MSD is tuned correctly. Check the nebulizer condition Clean the capillary entrance. Check the capillary for damage and contamination. Make sure drying gas flow and temperature are correct for the solvent flow. Make sure solvent is thoroughly degassed Make sure LC backpressure is steady; this indicates a steady solvent flow. Use appropriate mass filter values. Check spray shape. Nebulizer may be damaged or set incorrectly. Make sure drying gas flow and temperature are correct for the solvent flow. Make sure solvent is thoroughly degassed. Make sure LC backpressure is steady; this indicates a steady solvent flow. If you are using water as part of the mobile phase, make sure it is de-ionized (>18MW). Make sure nebulizing gas pressure is set high enough for the LC flow. Check position of needle in nebulizer. Stop solvent flow and remove nebulizer assembly. Examine end of nebulizer for damage. Make sure LC is on and there is sufficient solvent in correct bottle. Check for LC error messages. Check for blockages. Repair or replace any blocked components. Check for leaks. Make sure MS stream selector valve is set to LC to MSD. (APCI vs. Electrospray). APCI temperature is too high. Fragmentor is set too high.
Poor mass accuracy Low signal
Unstable signal
High spectral noise
Droplets, not spray, exiting the nebulizer
LC and LC/MS
No Flow
Undesired fragmentation
603
LC and LC/MS Applications

BioPharmaceutical
BioPharmaceutical
Combinatorial Chemistry: Fast Gradient Analysis and Re-equilibration Amino Acid Standards
1 2
Conditions: LC: Sample: Column: Gradient:
Sample: UV: Flow rate: Temperature:
Agilent 1100 Proprietary Combichem mixture Eclipse XDB - C18, 4.6 x 30mm (3.5 m), (P/N: 933975- 902) 0-100% B / 1 min. A= 50 mL H 2O + 450 mL MeOH + 2 mL H3PO4 B= 450 mL H 2O + 50 mL MeOH + 2 mL H3PO4 ~3 mg/mL each compound x 5 L = 15 g 268 nm 4.0 mL / min. 23C
Sample: Column: Mobile phase: Gradient: Flow rate: Temperature: Detection:
Amino Acid Standards 10. Val 1. Asp 1. 13Asp 11 11. Met 2. Glu 10 2.Glu14 3. Ser 12. Nva 3.Ser 5 13. Phe 4. His 4.His 14. Ne 5. Gly 3 15 5.Gly 15. Leu 6. Thr 8 6.Thr 16. Lys 7. Ala 7 9 4 6 17. ACA 8. Arg 7.Ala 18. Pro 9. Tyr 8.Arg 9.Tyr 18 10.Val 17 16 11.Met 12.Nva 13.Phe 0 to 18 minutes 14.Ne 15.Leu Amino acids (10 pmol each), AminoQuant derivatization 16.Lys Amino Acid (C18) column, 2.1 x 200 mm, 5 m, (P/N: 79916AA - 572) A = 20 mM sodium acetate, 0.018% TEA, pH 7.2, 0.3% THF 17.ACA B = 100 mM sodium acetate, pH 7.2, acetonitrile, methanol (1/2/2) 18.Pro
12 0 to 60% B in 17 min 0.45 mL/min 40C Fluorescence
AnalgesicsHigh-Speed
CombiHT Isocratic Separation
LC and LC/MS
CombiHT Columns Provide Fast Separations for Preparative Isocaratic Runs AnalgesicsHigh-Speed CombiHT Isocratic Separation 1.Acetaminophen (4-acetamidophenol) Analgesics (50 mg total): 2.Caffeine 1. Acetaminophen (4 - acetamidophenol) 2. Caffeine 3.2-Acetamidophenol 3. 2- Acetamidophenol 1 4.Acetanilide 2 4. Acetanilide 5.Aspirin (acetosalicylic acid) 5. Aspirin (acetosalicylic acid) 6.Phenacetin (acetophenetidin) 6. Phenacetin (acetophenetidin) 3 4 5 6 7
Separation of Small Molecule Anorectics on Bonus --RP and Traditional Alkyl Phase
1. Phentermine pK a 10.1 2. Fenfluramine pKa 9.1 Bonus - RP
Alkyl C8
Separation of Small Molecule Anorectics on Bonus--RPand Traditional Alkyl Phase 1. Phentermine 2. Fenfluramine
Column: Mobile Phase: Flow Rate: Temperature: Detection: Sample:
CombiHT ZORBAX SB - C18, 21.2 x 50 mm (P/N: 870050 - 902) 14% ACN / 1% Formic Acid 40 mL/min 70C 275 nm 50 L inj., 0.158 g/L each (caffeine 0.053 g/L)
Instrument: Columns: Mobile Phase: Flow Rate: Temperature: Detection: Injection Vol: Sample:
Agilent 1100 4.6 x 150 mm, 5 m (P/N: 883668-901) 25 mM K2HPO4, pH 7.2/MeOH: ACN (50:50), 45/55 1 mL/min. Ambient 254 nm 5 L Anorectics "Fen-phen"
Helpful Hint: For addiitonal pharmaceutical applications, please request Publication Number 5968-2635E, "Pharmaceutical Applications with HPLC." This publication can also be downloaded from our website at http://www.agilent.com/chem. Use the publication number to search.
604

BioPharmaceutical
Antibodies: Fast Separation of IgM and IgG Antibodies
Antibodies: Fast Separation of IgM and IgG Antibodies 1.IgM, MOPC-104E 2.IgG2a, l HOPC-1 3.Buffer Solution
1. IgM, MOPC - 104E 2. IgG2a, l HOPC - 1 3. Buffer Solution
b-blocker Drugs: Silica-Based Column Aging at pH 11

-blocker Drugs: Silica-Based Column Aging at pH 11 1.Pindolol 1. Pindolol 2.Metprolol 2. Metprolol 3.Oxprenolol 3. Oxprenolol 4.Propranolol
4. Propranolol
0 Sample: Column: Mobile Phase: Flow Rate: Temperature: Detection: 2.5 l (1mg/mL) ZORBAX GF- 250, 4.6 x 250 mm, (P/N: 884973 - 701) 200 mM Sodium Phosphate (pH 7), 0.01% Azide 0.94 mL/min Ambient UV 230 nm
10
12
14
16
18
20
22
24
Sample: Column: Mobile Phase: Flow Rate: Temperature: Detection:
- blocker drugs
ZORBAX Eclipse XDB - C8, 4.6 x 150 mm, 5 m, (P/N: 993967- 906) 55% MeOH / 45% 1- Methyl- piperidine - HCL buffer, pH 11 1.0 mL/min 24C UV 215 nm
Antidepressants: Separation of Highly Basic Antidepressants above their pKa in Free Base Form (pKa 9.5-9.7)
Sample: I. Impurity 1. Doxepin Antidepressants: Separation of Highly Basic 2. Imipramine Antidepressants above their pKa in Free Base 3. Nortriptyline 9.5-9.7) 4. Amitriptyline 1.Doxepin 5. Trimipramine
Heparin
LC and LC/MS
Form (pKa
2.Imipramine 3.Nortriptyline 4.Amitriptyline 5.Trimipramine
Column: Mobile Phase: Temperature: Flow Rate: Detection: Column: Mobile Phase: Flow Rate: Flow Rate: Temperature: Detection: ZORBAX Extend - C18 4.6 x 150 mm, 5 m (P/N: 773450-902) 75% Methanol / 25% 50 mM Pyrrolidine Buffer, pH 11.5 0.5 mL/min. Panel A: 1.5 mL/min.; Panel B: 1.0 mL/min. 40C UV at 215 nm
3 x PL aquagel-OH 30 in series, 7.5 x 300 mm, 8 m (P/N: 79911GF-083) 0.2 M NaNO3, NaH2PO4, pH 7 25C 1 mL/min. Refractive Index Detector
Basic drugs can often be separated in their charged form at low pH with StableBond or at mid- range pH with Eclipse XDB or Bonus - RP columns. With Extend-C18, you can separate at high pH to improve solubility, improve retention, or obtain different selectivity .
605

BioPharmaceutical
Antidepressants, Tricyclic: Comparative Separation
Antidepressants, Tricyclic: Comparative Separation 1.Propanolol 2.Doxepin 3.Nortriptyline Bonus-RP 4.Amitriptyline (P/N: 883668-901) 5.Trimipramine 1. Propanolol
2. 3. 4. 5. Doxepin Nortriptyline Amitriptyline Trimipramine
Brand B Polar- linked C16
Brand A Polar- linked C8
Instrument: Column: Mobile Phase: Flow Rate: Temperature: Detection: Sample:
Agilent 1100 4.6 x 150 mm ACN: 20 mM Na Citrate, pH 6 (60:40) 1.0 mL/min. Ambient 254 nm u = uracil
Cephalosporins
Cough-formula Mixture Fast and Efficient Separation

1. 2. 3. 4. 5. 6. 7. 8. Cefachlor Cefazolin Cefatoxime Cefalexin Cefoxitin Cafoperazone Cefamandole nafate Cephalothin
LC and LC/MS
Cephalosporins 1.Cefachlor 2.Cefazolin 3.Cefatoxime 4.Cefalexin 5.Cefoxitin 6.Cafoperazone 7.Cefamandole nafate 8.Cephalothin
Cough-formula MixtureFast and Efficient Separation ZORBAX SB - CN 1. Maleic acid 1. Maleic acid 4.6 x 75 mm, 3.5 m 2. Pseudo-ephedrine 2. Pseudo - ephedrine P/N: 866953- 905 3. Acetominophen 3. Acetominophen Flow=1.5 mL/min 4. Chlorpheniramine 4. Chlorpheniramine Run Time=3.5 min 5. Dextromethorphan 5. Dextromethorphan
ZORBAX SB - CN 4.6 x 150 mm, 5 m P/N: 883975- 905 Flow=1.0 mL/min Run T ime=9.5 min
Cephalosporin Mixture ZORBAX C8, 4.6 x 150 mm, 5 m, (P/N: 883952 - 706) 93% buffer (50 mM citric acid + LiOH to pH 3) 0.8% ACN, 6.2% THF 3.0 mL/min. 50C UV 254 nm
2 L, Cough Formula ZORBAX SB - CN, see above 4.6 x 75 mm, 5 m 20/80, Acetonitrile/150 mM NaCitrate, pH 2.6 1.5 mL/min, 1.0 mL/min 35C UV 270 nm
606

BioPharmaceutical
dsDNA Restriction Digest Separations
A. 2 g pUC 18/Msp I + 1.5 g x 174 Hae III
Gradient: 45-75% B in 90 minutes
B. pBR 322/Hae III digest

Instrument: Column: Mobile Phase: Temperature: Flow Rate: Sample: Agilent 1100, 1 mL flow cell, binary pump A. Eclipse dsDNA, 2.1 x 150 mm, (P/N: 993850-902) B. Eclipse dsDNA, 4.6 x 75 mm, (P/N: 992750-902) A: 0.1 M TEAA*, 0.1 mM EDTA, pH 7.0 B: A with 25% acetonitrile 50C A: 0.2 mL/min B: 1.0 mL/min 200 ng restriction digest standards Gradient: 40-80% B in 30 minutes
*TEAA = triethylammonium acetate
Eclipse dsDNA Reproducibility
Nucleosides: Separation of Deoxy and Ribonucleosides

Nucleosides: Separation of Deoxy and Ribonucleosides 1.Cytidine 1. Cytidine 2.2' Deoxycytidine 2. 2' Deoxycytidine 3.Inosine 3. Inosine 4.Guanosine 4. Guanosine 5.2' Deoxyinosine 5. 2' Deoxyinosine 6.2' Deoxyguanosine 6. 2' Deoxyguanosine 7. Adenosine 7.Adenosine
LC and LC/MS
Retention Time (min) Conditions: Instrument: Column: Mobile Phase: Gradient: Temperature: Flow Rate: Sample: Agilent 1100, 1 L flow cell, binary pump Eclipse dsDNA, 2.1 x 75 mm, (P/N: 945750-902) A: 0.1 M TEAA, 0.1 mM EDTA, pH 7.0 B: A with 25% acetonitrile 50-75% B in 10 minutes 50C 0.3 mL/min 420 ng pUC 18 HAE III restriction digest
Sample: Column: Mobile phase: Flow rate: Temperature: Detection:
2 L (1.6 g each) ZORBAX SB - C3, 4.6 x 150 mm, 5 m, (P/N: 883975 - 909) 4mM Ammonium Phosphate (pH 4.0 with Phosphoric Acid) 2.0 mL/min 35C UV 254 nm
607

BioPharmaceutical
DHPLC of p53 Exon 8 (200 bp G-->T)
Column: Eclipse dsDNA Analysis Column, 2.1 x 75 mm, (P/N: 945750-902) Flow Rate: 0.4 mL/min (2.1 mm id) Mobile Phase: A: 100 mM TEAA pH 7.0, 0.1 mM EDT A B: 25% Acetonitrile, 100 mM TEAA pH 7.0, 0.1 mM EDTA Gradient: 50-60% B @ 1.8%/min. Predicted Temp: 64.4C Actual Temp: 63C Detector: UV/VIS 260 nm, 10 nm bandwidth Ref: 360 nm, 50 nm bandwidth High Pressure Micro Flow Cell; 1.7 L, 6 mm path length Sample: p53
Homoduplex wild type
Heteroduplex
Because these samples are clearly different, the next step would be to find the precise mutation by DNA sequencing. When that was done, the mutation found was a G to T transition (i.e., a T was substituted for the original G in the wild type DNA sequence).
Nucleotides: Separation of Mononucleotides

Nucleotides: Separation of Mononucleotides 1.Orotic Acid 1. Orotic Acid 2.UMP 2. UMP 3.GMP 3. GMP 4.XMP 4. XMP
Oligonucleotides: Separation of Standard p(DT) 12-18
LC and LC/MS
Sample: Column: Mobile Phase: Flow rate: Temperature: Detection:
Orotic Acid, MP, GMP, XMP ZORBAX SAX, 4.6 x 250 mm, 5 m, (P/N: 880952 - 703) 0.1 M NH4H 2PO4 2 mL/min Ambient UV 254 nm
Sample: Column: Mobile Phase: Gradient: Flow rate: Temperature: Detection:
50 L, 5 units/mL ZORBAX Oligo, 6.2 x 80 mm, 5 m, (P/N: 820940 - 901) A: 20/80 ACN / 20 mM Sodium Phosphate pH 7.0 B: 20/80 ACN / 20 mM Sodium Phosphate pH 7.0 + 0.8 M NaCI 35- 80% B in 90 min 1.0 mL/min Ambient UV 260 nm, 2.0 AUF
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BioPharmaceutical
Oligonucleotides: Separation of 24 and-25 mer
Separation of Basic Peptides on Bonus-RP versus Traditional Alkyl Phase
(P/N: 883668-901)
Instrument: Columns: Mobile Phase: Gradient: Injection: Flow Rate: Temperature: Detection: Sample:
1.0 mL, Oligonucleotide sample ZORBAX Oligo, 6.2 x 80 mm, 5 m, (P/N: 820940 - 901) A = 20% ACN / 80% 0.02 M Sodium Phosphate B = A + 1.0 NaCl 35% B to 60% B in 90 min 1.0 mL/min Ambient UV 260 nm
Agilent 1090 4.6 x 150 mm A: 0.010 M ammonium phosphate, pH 7 / 0.050 M sodium perchlorate B: 0.010 M ammonium phosphate / 0.050 M sodium perchlorate in 50% ACN 0-100% B in 50 min. 10 L 1 mL/min. 40C 215 nm Basic 11- residue peptides with net +1, +2, +3, +4 positive charges at neutral pH C1: Ac - Gly - Gly - Gly - Leu - Gly - Gly - Ala - Gly - Gly - Leu - Ly s - amide C2: Ac - Ly s - Ty r- Gly - Leu - Gly - Gly - Ala - Gly - Gly - Leu - Ly s - amide C3: Ac - Gly - Gly - Ala - Leu - Ly s - Ala - Leu - Ly s - Gly - Leu - Ly s - amide C4: Ac - Ly s - Ty r- Ala - Leu - Ly s - Ala - Leu - Ly s - Gly - Leu - Ly s - amide
Peptides: Separation of Antiotensins I, II, III with TFA and NH4OH3

Acidic Conditions A - 0.1% TFA in water B - 0.085% TFA in 80% ACN Basic Conditions A - 10 mM NH 4OH in water B - 10 mM NH 4OH in 80% ACN
LC and LC/MS
Column: Flow Rate: Temperature: Mobile Phase: Gradient: LC/MS: Sample:
ZORBAX Extend - C18, 2.1 x 150 mm, 5 m (P/N: 773700-902) 0.2 mL/min 35C As indicated 15-50% B in 15 min Pos. Ion ESI - Vf 70V, Vcap 4.5 kV N2- 35 psi, 12 L/min, 325 C 2.5 L sample (50 pmol each)
609

BioPharmaceutical
Peptides: Comparison of Mass Spectra of Angiotensin I with TFA and NH4OH Mobile Phases
Max: 10889
Acidic Conditions A-0.1% TFA in water B-0.085% TFA in 80% AcN
Max: 367225
Basic Conditions A-10 mM NH 4OH in water B-10 mM NH 4OH in 80% AcN
Column: Flow Rate: Temperature: LC/MS: Sample:
ZORBAX Extend-C18, 2.1 x 150 mm, 5 m (Part No. 773700-902) 0.2 mL/min 35C: 15-50% B in 15 min Pos. Ion ESI-Vf 70V, Vcap 4.5 kV, N2-35 psi, 12 L/min, 325C 2.5 L Angiotenision I (50 pmol)
LC and LC/MS
Peptides: Aspartate Aminotransferase Tryptic Digest

Aspartate Aminotransferase Vydac C - 18, 300, 1.0 x 250 mm, 5 m, (P/N: 79918O3- 581) Mobile phase: A = Water, 0.05% TFA B = Acetonitrile, 0.045% TFA Gradient: 0 min 1% B 85 min 30% B 95 min 70% B 100 min 98% B 110 min 1% B Flow rate: 0.050 mL/min Injection volume: 10 L, 50 pmol Detection: UV 280 nm Sample: Column: Peak Rt (min) 31.4 36.2 45.0 63.0 66.5 78.2 86.1 101.8 St. Dev (min) 0.080 0.088 0.054 0.047 0.064 0.079 0.036 0.061 RSD % 0.25 0.24 0.12 0.07 0.10 0.10 0.04 0.06
Peptides: Effect of TFA Concentration

Peptide Sequences 0.05 % TF A
0.25 % TF A
1.0 % TF A
9 8 Absorbance 280 nm 7 6 5 4 3 2 1 0 30 40 50 60 70 80 90 100 110 min. Gradient: Flow rate: Temperature: Detection: Injection: Sample: Column: Mobile phase: Peptide Standards S1- S5, decapeptides differing slightly in hydrophobicity ZORBAX 300SB - C8, 4.6 x 150 mm, 5 m, (P/N: 883995 - 906) A = Water and TFA B = ACN and TFA 0 - 30% B in 30 min. 1.0 mL/min 40C UV 254 nm 6 L Peptide Standards
610

BioPharmaceutical
Peptides/Proteins: Equivalent Gradient Separations
1. Met- enkephalin 2. Leu - enkephalin Peptides/Proteins: 3. Angiotensin II 1.Met-enkephalin 4. Neurontensin 5. RNase 6. Insulin (BOV) Equivalent Gradient 7. Lysozyme 8. Calmodulin 6 5 4 8 7 9 10
Separations
9. Myoglobin 10. Carbonic anhydrase

mAU 1400 1200 1000 800 600 400 200
Reducing Capillary ID to Improve Sensitivity for Concentration-Limited Samples
2.Leu-enkephalin 3.Angiotensin II 2 1 4.Neurontensin Analytical 3 5.RNase 1 mL / min 6.Insulin (BOV) 7.Lysozyme 8.Calmodulin 9.Myoglobin 10.Carbonic anhydrase
Narrow Bore 0.2 mL / min 2 1
Column ID
0.3 mm 0.5 mm 1.0 mm 4.6 mm
6 5 7 9 8 10 Column: ZORBAX SB-C18, 150 mm, 5 m Mobile Phase: 60% ACN/40% H2O Flow Rate: 0.3 mm id - 4 L/min. 0.5 mm id - 10 L/min. 1.0 mm id - 50 L/min. 4.6 mm id - 1.0 mL/min. Temperature: Ambient Detection: 254 nm Sample: 200 ng Biphenyl (100 nL)
0
34
Sample: Column: Mobile Phase: Gradient: Flow Rate: Temperature: Detection:
10 L injection, Concentration 2- 6 g ZORBAX 300SB - C8, 4.6 x 150 mm, 5 m, (P/N: 883995 - 906) ZORBAX 300SB - C8, 2.1 x 150 mm, 5 m, (P/N: 883750 - 906) A = 95% Water: 5% ACN with 0.1% TFA B = 5% Water: 95% ACN with 0.085% TFA 10- 60% B in 30 min. as indicated 35C UV 215 nm
LC and LC/MS
Peptides/Proteins: Effect of Elevated Temperature

1. Leucine Enkephalin 2. Angiotensin II Peptides/Proteins: 3. RNase A Effect of 1.Leucine4.Enkephalin Insulin (BOV) 5. Cytochrome C 6. Lysozyme Elevated Temperature 7. Myoglobin 8. Carbonic anhydrase
Capillary LC Analysis of a Tryptic Digest

DAD 2nd Derivative Spectra for ID
Tyr Trp
2.Angiotensin II 3.RNase A 4.Insulin (BOV) 5.Cytochrome C 6.Lysozyme 7.Myoglobin 8.Carbonic anhydrase

4 5
Absorption
206 nm
280 nm
Time (min.)
Column:
Polypeptides ZORBAX 300 SB - C3, 4.6 x 150 mm, 5 m, (P/N: 883995 - 909) A = 5:95 ACN:Water with 0.10% TFA (v/v%) B = 95:5 ACN:Water with 0.085% TFA (v/v%) 15- 53% in 20 min., posttime 12 min. 1.0 mL/min Ambient - 60C UV 215 nm
ZORBAX 300 SB-C18, 0.3 x 250 mm, 5 m, (P/N 5065-8265) Mobile Phase: A - 0.05% TFA in Water B - 0.045% TFA in Acetonitrile Gradient: 5-60% B in 45 min Flow Rate: 4 L/min Sample: 20 fmol injected
611

BioPharmaceutical
Peptide Map Electronic Flow Control Reproducibility of 10 Injections on Capillary LC Capillary LC/ESI-MS Concentration Effects on Detection of a Protein Digest
10000
250 x 2.1 mm id
TIC 3003000 m/z
30000
250 x 1.0 mm id
RSD RT
600000
250 x 0.30 mm id
Column:
ZORBAX 300SB-C18, 0.3 x 250 mm, 5 m (P/N 5064-8265) Mobile Phase: A - 0.05% TFA in Water B - 0.045% TFA in Acetonitrile Sample: 1.3 L (myoglobin tryptic digest, 7.5 pmol/L) Gradient: 0.5% B/min. Flow Rate: 5.5 L/min. Temperature: 30C Detection: 206/10 nm, 450/80 nm
Column: ZORBAX 300SB-C18, 5 m Gradient: 060% B/60 min. Mobile Phase: A 0.1% TFA in water B 0.1% TFA in AcN Sample: 6.6 pmol (5 L) of LysC digest of DSPA
Poroshell 300SB-C18, 2.1 x 7.5 mm
Fast, High-Resolution Separation of Peptides and Proteins with Poroshell 300SB-C18

Sample (peptides/proteins) 1. gly-tyr 0.125 mg/mL 2. Val-tyr-val 0.5 mg/mL 3. Met-enkephalin 0.5 mg/mL 4. Leu-enkephalin 0.5 mg/mL 5. Angiotensin II 0.5 mg/mL 6. RNAse A 1 mg/mL 7. Cytochrome C 1 mg/mL 8. Holotransferrin 1 mg/mL 9. Apomyoglobin 1 mg/mL
6 7 8 9
LC and LC/MS
DAD1 A, Sig=215, 16 Ref=310, 10 (A:\PSHLL022.D)

300 250 200 150 100 50 0 -50 0 0.1 0.2 0.3 0.4
2 1
4 5
1. Angiotensin II 2. Neurotensin 3. RNase 4. Insulin 5. Lysozyme 6. Myoglobin 7. Carbonic Anhydrase 8. Ovalbumin
0.5
0.6
0.7
min.
A=0.1% TFA, H2O; B=0.07% TFA, AcN 0100%B/1.33 min., Flow=3 mL/min., Temp.=70C, Inj 0.5 L sample Mixer bypassed with P/N: G1312-67301; Loop-bypass program
Spaces between solutes indicate good peak capacity for rapidly separating complex samples.
Column: Poroshell 300SB-C18, 2.1 x 75 mm Mobile Phase: A 0.1% TFA B 0.07% TFA in AcN Gradient: 5100% B in 1.0 min. Flow Rate: 3.0 mL/min. Temperature: 70C Pressure: 260 bar Detection: UV, 215 nm
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BioPharmaceutical
Peptide RP-HPLC/ESI-MS Using NH4OH Mobile Phase Yields Both Positive and Negative Ion Spectra
LLL-NH2 LHL LLG
TIC 1501500 m/z TFA Conditions, 25C A- 0.1% TFA in water B- 0.085% TFA in 80% AcN 33-45% B in 30 min. TFA Conditions, 80C A- 0.1% TFA in water B- 0.085% TFA in 80% AcN 29-41% B in 30 min. NH4OH Conditions, 25C A- 20 mM NH4OH in water B- 20 mM NH4OH in 80% AcN 26-38% B in 30 min.
High pH Can be Used for Separating Hydrophobic or Other Low-Solubility Peptides

Comparison of A Peptide RP-HPLC Separations at Low and High pH
Conditions: 5 L sample (100 pmol each); 300 ZORBAX Extend-C18,
Positive Ions
LLVF LLL
2.1 x 150 mm; 0.25 mL/min.

Amyloid Sequences: Asp Ala Glu Phe Arg His Asp Ser Gly Tyr Glu Val His His Gln Lys Leu17 Val Phe Phe Ala Glu Asp Val Gly Ser Asn Lys Gly Ala Ile Ile Gly Leu Met Val Gly Gly38 Val Val40 Ile Ala42 Thr43-COOH At 25C and low pH, the recovery of A(1-42) and (1-43) is very poor (5-10%). Retention of all peptides were lower at high pH, for any given temperature. Resolution of the A(1-42) and (1-43) peptides could be obtained at low pH/high temperature, or at high pH at lower temperature. The retention orders of A(1-42) and (1-43) are reversed at low versus high pH.
A(1-38) A(1-40)
Negative Ions LHL LLL-NH2 LLL LLG
Absorbance (210 mm)
A(1-42/3)
A(1-43) A(1-40) A(1-42)
LLVF
A(1-38)
A(1-42) A(1-40) A(1-38)
A(1-43)
Column: Flow Rate: Temperature: Gradient: LC/MS: Sample:
ZORBAX Extend-C18, 2.1 x 150 mm 0.25 mL/min. 25C 5-60% B in 20 min. Pos. Ion ESI- Vf 70V, Vcap 4.5 kV, N2- 35 psi, 12 L/min., 300C 4 L (50 ng each peptide)
Selectivity Comparison of TFA and NH4OH for Peptide RP-HPLC\ESI-MS Analysis
LC and LC/MS
0.1% TFA TFA Conditions: A 0.1% TFA in water B 0.085% TFA in 80% AcN
LLL-NH2 LLG TIC 1501500 m/z LHL LLL
LLVF
20 mM NH4OH
LLG LLL
LLL-NH2
LHL
LLVF
NH4OH Conditions: A 20 mM NH4OH in water B 20 mM NH4OH in 80% AcN
Column: Flow Rate: Temperature: Gradient: LC/MS: Sample:
ZORBAX Extend-C18, 2.1 x 150 mm 0.25 mL/min. 25C 5-60% B in 20 min. Pos. Ion ESI- Vf 70V, Vcap 4.5 kV, N2- 35 psi, 12 L/min., 300C 4 L (50 ng each peptide)
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BioPharmaceutical
Proteins: Effect of Bonded Phase
ZORBAX 300SB- C8 GRADIENT: 23- 48% ACN 1.
ZORBAX 300SB- CN GRADIENT: 25- 43% ACN 2.
Wheat Proteins, including w - gliadins ZORBAX 300SB - C8, 300SB - CN, 4.6 x 150 mm, 5 m, (P/N: 883995- 906 883995- 905) A = 0.1% TFA in Water, B = 0.1% TFA in 50/50 ACN/Water 1. 46- 96% B in 60 min. 2. 50- 86%B in 60 min. 1.0 mL/min 50C UV 210 nm
LC and LC/MS

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BioPharmaceutical
Proteins: Effect of Flow Rate
Rs = 2.3 N = 15,680 1. 2. 3. 4. 5. BSA - dimer BSA Ovalbumin Lysozyme Sodium Azide
Proteins: Effect of Bonded Phase

300SB - C18
Run T ime = 60 min Flow = 0.25 mL/min
300SB - C8 Rs = 2.1 N = 11,939 Run T ime = 30 min Flow = 0.5 mL/min 300SB - C3 Rs = 1.9 N = 8,913 Run T ime = 15 min Flow = 1 mL/min 300SB - CN Rs = 1.7 N = 6,623 Run T ime = 7.5 min Flow = 2 mL/min
Rs = 1.4 N = 3,588
Run T ime = 3.5 min Flow = 5 mL/min
Sample: Column:
1 mg/mL each protein, 0.1 mg/mL azide, 10 L ZORBAX GF- 250, 9.4 x 250 mm, 4 m (P/N: 884973 - 901) 200 mM Sodium Phosphate, pH 7.0 As indicated Ambient UV 225 nm
Mobile phase: Gradient: Flow rate: Temperature: Detection: Injection:
Polypeptides ZORBAX 300SB - C18, 300SB - C8, 300SB - C3, 300SB - CN, 4.6 x 150 mm, 5 m, (P/N: 883995- 902, 883995- 906, 883995- 909 883995- 905) A = 0.1% TFA in H2O, B = 0.09% TFA in 80% ACN/20% Water 25- 70% B in 40 min 1.0 mL/min 60C UV 210 nm 3 g each polypeptide
LC and LC/MS
615

BioPharmaceutical
Proteins: Isolation of Aspartate Aminotransferase
Proteins: Separation using SynChropak CM 300 Column Mixture

1 2 3
100 90 80 70 60 50 40 30 20 10 0 5 10 15 Time (min.) 20 25 0 Sample: Column: Mobile phase: Gradient: Flow rate: Temperature: Detector: 100 L containing 150 g of crude E. coli extract PL 1000 - SAX, 5.0 x 50 mm, 8 m, (P/N: 79931QA - 835) A = 10 mM Hepes, 1 mM DTE, 1 mM EDTA, pH 7.0; B = Same as A with 1.0 M NaCl 0 to 5 min 0% B, 5 to 25 min 30% B, 1 mL/min Ambient, 25 to 30 min to 100% B UV at 360/280 nm Sample: Column: Mobile phase: Gradient: Flow rate: Detection: 6 12 18 280 nm 360 nm
Proteins: Separation using SynChropak CM 300Column Mixture 1. BSA 1.BSA 2. Cytochrome C 2.Cytochrome C 3. Lysozyme 3.Lysozyme
24 min.
Protein SynChropak CM300, 300, 4.6 x 100 mm, 6.5 m, (P/N: 79919CM- 754) A = 0.01 M sodium phosphate, pH 6.0 B = 0.01 M sodium phosphate in 0.5 M NaCl, pH 6.0 0 to 100% B in 30 min 1.0 mL/min UV 254 nm
Proteins: Separation by SEC
Proteins: Calibration Standard Mix of Proteins GF-250

Proteins: Calibration 1. Thyroglobin 6.69 kd 2. ADH 150 kd 1.Thyroglobin 3. BSA 67 kd 2.ADH 4. Ovalbumin 43 kd 3.BSA 5. Myoglobin 17.8 kd 4.Ovalbumin 6. Azide 0.045 kd 5.Myoglobin 6.Azide Standard Mix of Proteins GF-250
LC and LC/MS
1. 2. 3. 4. 5. 6. 7.
Proteins: SEC D Mouse iGM Separation by 900,000 1.Mouse iGM Bovine thyroglobin 670,000 D Sweet potato thyroglobin b - amylase 200,00 D 2.Bovine Bovine serumpotato albumin b-amylase 67,000 D 3.Sweet Chicken albumin 45,000 D 4.Bovine serum albumin Bovine RNAase 13,700 D 5.Chicken albumin Azide 65 D 6.Bovine RNAase 7.Azide
2 3 4 5 6
4 5
7 8 9 10 1 1 12 13 14 15 16 17 18 19 20 Retention Volume (ml)
Proteins ZORBAX GF - 250, 9.4 x 250 mm, 4 m (P/N: 884973 - 901) 130 mM NaCl, 20 mM KCI, 50 mM Na 2 PO4 , pH 7.0 1 mL/min Ambient UV 210 nm
Calibration Curve for ZORBAX GF-250 Sample: Lyophilized proteins Column: ZORBAX GF-250, 9.4 x 250 mm, 4 m (P/N: 884973- 901) Mobile phase: 0.2M sodium phosphate, pH 6.8; Flow rate: 1.0 mL/min Temperature: Ambient (21 C) Detection: UV 230 nm
616

BioPharmaceutical
Proteins: SEC Characteristic Elution Profiles of Basic and Hydrophobic Proteins
Nucleotides: Using Rapid Separation 3.5 m Columns

5 m 4.6 x 150 mm (P/N: 883975- 905) T C A 6 min.
3.5 m 4.6 x 50 mm (P/N: 835975- 905) 2 min.
Bovine Insulin, Lysozyme, Cytochrome C ZORBAX GF-250, 9.4 x 250 mm, 4 m (P/N: 884973-901) Sodium Phosphate, pH 7.0 concentration as indicated 2 mL/min Ambient UV 230 nm
Column: Mobile Phase: UV: Flow:
ZORBAX SB - CN A: 0.1% TFA B: 97.5% MeOH : 2.5% H2O (0.1% TFA) 254 nm; 1.0 mL/min.; 30 C
Proteins: Ion Exchange Separation of Commercial Ovalbumin
Proteins: Basic Proteins Separated using Ion Exchange
LC and LC/MS
1. RNA polymerase 2. Chymotrypsinogen 3. Lysozyme
Proteins: Basic Proteins Separated2using Ion Exchange 3 1.RNA polymerase 1 2.Chymotrypsinogen 3.Lysozyme
15
30
min.
10
20
30 min.
Sample: Column: Mobile phase: Gradient: Flow rate: Detection:
Sample: Column: Mobile phase: Gradient: Flow rate: Detection:
Ovalbumin SynChropak AX300, 4.6 x 100 mm, 6.5 m, weak anion exchanger, (P/N: 79919DE-754) A = 0.02 M tris, pH 7.0 B = 0.02 M tris in 1.0 M sodium acetate, pH 7.0 0 to 100% B in 30 min 1.0 mL/min UV 254 nm
Basic Proteins SynChropak SCX, 4.6 x 100 mm, 6.5 m, strong cation exchanger (P/N: 79919SP-754) A = 0.02 M tris, pH 7.0 B = 0.02 M tris in 0.5 M sodium acetate, pH 7.0 0 to 100% B in 30 min 1.0 mL/min UV 254 nm
617

Chemical/Industrial
Chemical/Industrial
Alkyd Resin: Gel Permeation Chromatography using PLgel Columns Chelating Agents using Purospher
Chelating Agents using Purospher 1.Uracil 1 2.Acetylacetone 3.Salicylamide 4.2,2'-Bipyridine 4
1. 2. 3. 4.
Uracil Acetylacetone Salicylamide 2,2'- Bipyridine
Poor quality alkyd resin Good quality alkyd resin
2 3
12
16
20 min.
Sample: Column: Sample: Column: Mobile phase: Flow rate: Temperature: Detection: Alkyd Resins 3 PLgel columns in series, 100 , 500 , 104 , 5 m, (P/N: 79911GP - 501 & 502 & 504) Tetrhydofuran 1.5 mL/min Ambient Refractive Index HP 1037A Mobile Phase: Flow Rate: Temperature: Detection:
Chelating Agent Purospher RP- 18, 4 x 125 mm, 5 m, (P/N: 79925PU - 564) 30/70 Acetonitrile/Water 1.0 mL/min Ambient 254 nm
LC and LC/MS
Dextrans Characterization using GPC
Epoxy Resins: Detection Comparison

Epoxy Resin Epikote 1001 Epikote 1004 Epikote 1007 Mw data 3468 5928 10896 Mn data 1101 2042 3851 Polydispersity 3.149 2.901 2.829
Dextrans ZORBAX PSM - 60, PSM - 300, 6.2 x 250 mm, 5 m, (P/N: 880957- 801, 880957- 805) 100 mM Sodium Acetate (pH 6.0-6.5) with H3 PO4 0.5 mL/min 30C RI
Epoxy Resin Sample dissolved in 1 mL THF 2x PLgel Mixed - D, 7, 5 x 300 mm, 5 m, (P/N: 79911GP - MXD) Tetrahydrofurane (THF) 1.0 mL/min 20C DAD 254/100 nm, & refractive index, reference 360/100 nm
618

Chemical/Industrial
Polybutadiene: GPC using UV and Refractive Index
Refractive Index
Mp = 10283 Mn = 6567 Mw = 13565 Polydispersity = 2.066 Mz = 22037 Mz+1 = 35784 Mv = 12579
Polyethylene: Separation by GPC
UV Detection
Mp = 10000 Mn = 10543 Mw = 12054 Polydispersity = 1.143 Mz = 14804 Mz+1 = 21860 Mv = 1 1780
Time (min)
33 mg Polybutadiene sample dissolved in 1 mL THF, 20 L/injection 2x PLgel Mixed - D, 7.5 X 300 mm, 5 m, (P/N: 79911GP - MXD) Tetrahydrofurane (THF) 1.0 mL/min 20C DAD 254/100 nm & refractive index, reference 360/100 nm
Commercial polyethylene ZORBAX PSM Bimodal Set, 6.2 x 250 mm, 5 m, (P/N: 880957- 814) o- Dichlorobenzene 0.75 mL/min 140C Infrared (3.4 m)
Polymethacrylate GPC Separation
Polystyrenes, Differently Processed: GPC Analysis

MWdata Mp Mn Mw Polydispersity Mz Mz+1 Mv Granulate 109776 59152 159590 2.698 327846 545718 141380 Chips 87563 49062 133565 2.722 297500 539583 117205 Chips, grinded and injection molded a 2nd time 103049 55036 149385 2.714 311084 533941 132243
LC and LC/MS
GOOD
Granulate BAD Chips Chips ground and injection molded a 2nd time
33 mg Polymethacrylate sample dissolved in 1 mL THF ZORBAX Bimodal-S, 6.2 x 250 mm, 5 m, (P/N: 880957- 814) Tetrahydrofuran (THF) 1.0 mL/min 35C UV 254 nm
Polystyrenes dissolved in 1 mL THF , 10 L/injection 3x PLgel Mixed B columns, 7.5 X 300 mm, 5 m, (P/N: 79911GP - MXB) Tetrahydrofurane (THF) 1.0 mL/min 20C RI
619

Chemical/Industrial
Polyurethane Resins Triton X-114: Decreasing Run-time by Changing Bonded Phase
ZORBAX SB-C3 (P/N: 883975-909) 70/30% MeOH/H20
GOOD
BAD
ZORBAX SB-C18 (P/N: 883975-902) 75/25% MeOH/H20
Polyurethane Resins ZORBAX PSM 60S, 6.2 x 250 mm, 5 m, (P/N: 880957- 802) Dimethyl Formamide 1.9 mL/min Ambient RI
Triton X - 114 ZORBAX SB - C3, SB - C18, 4.6 x 150 mm, 5 m, (P/N: 883975- 909, 883975 - 902) MeOH and H 2O (as indicated) 1.0 mL/min 50C UV 225 nm
LC and LC/MS
y PMMA Polymer with MW = 316000
Polyethoxylate
Column: Mobile Phase: Flow Rate: Oven Temperature: Injection Volume: Detection: Sample: Calibrant:
3 x PLgel Mixed-C in series, 7.5 x 300 mm, 5 m (P/N: 79911GP-MXC) Tetrahydrofuran (THF) 1.0 mL/min 20C 10 L Refractive Index Detector and Diode Array Detector 26 mg dissolved in 1 mL THF EasyCal Polystyrene standards in vials were used for narrow standard calibration
Column: Mobile Phase: Column Temp: Flow Rate: Detector: Calibrant:
PL aquagel-OH Mixed, 7.5 x 300 mm, 8 m (P/N: 79911GF-MXA) in series with PL aquagel-OH 30, 7.5 x 300 mm, 8 m (P/N: 79911GF-083) Water 25C 1 mL/min Refractive Index Detector Polyethylene oxide EasyCal standards in vials for calibration
620

Chemical/Industrial
Polyvinyl Alcohol
Polyvinyl Alcohol
Organic Acids Separated on ZORBAX SB-Aq
1. Lactic Acid 2. Acetic Acid 3. Citric Acid 4. Fumaric Acid 5. Succinic Acid
Column: Mobile Phase: Temperature: Flow Rate: Detection:
3 x PL aquagel-OH 30 in series, 7.5 x 300 mm, 8 m (P/N: 79911GF-083) 0.2 M NaNO3, NaH2PO4, pH 7 25C 1 mL/min Refractive Index Detector
Column: Mobile Phase: Flow Rate: Temperature: Detection:
ZORBAX SB-Aq, 4.6 x 150 mm, 5 m (P/N: 883975-914) 99% 20 mM NaHPO4, pH2, 1% ACN 1.0 mL /min 35C UV (210 nm)

LC and LC/MS
621

Environmental
Environmental
DNPH-Derivatized Aldehydes Obtained from Air
DNPH-Derivatized Aldehydes Obtained from Air 2. Acetaldehyde - DNPH 1.Formaldehyde DNPH 3. Acetone - DNPH 2.Acetaldehyde-DNPH 4. Acrolein - DNPH 5. Propionaldehyde - DNPH 3.Acetone-DNPH 6. Crotonaldehyde - DNPH 4.Acrolein-DNPH 1 7. 2- Butanone (MEK) - DNPH 5.Propionaldehyde-DNPH 8. Methacrolein- DNPH 6.Crotonaldehyde-DNPH n- Butyraldehyde - DNPH 9. Benzaldehyde - DNPH 7.2-Butanone (MEK)-DNPH 10. Valeraldehyde - DNPH 2 8.Methacrolein-DNPH 11. m- Tolualdehyde - DNPH 8 n-Butyraldehyde-DNPH 12. Hexanaldehyde - DNP 3 4 5 9.Benzaldehyde-DNPH 6 7 10.Valeraldehyde-DNPH 10 12 9 11 11.m-Tolualdehyde-DNPH 12.Hexanaldehyde-DNP
0 5 Sample: Column: Mobile phase: Gradient: Flow rate: Temperature: Detection: 10 15 20 25 30 min. 1. Formaldehyde DNPH
Amines, Substituted
Amines, Substituted 2. 4 10. 2- Ethyl - 6- methylaniline - Chloroaniline 3. 3- Chloroaniline 11. 3,4- Dichloroaniline 1.3-Cl-4-methoxyaniline 4. 2- Chloroaniline 12. 4- Isopropylaniline 2.4-Chloroaniline 5. 2,6- Dimethylaniline 13. 2,4- Dichloroaniline 3.3-Chloroaniline 6. 3- Cl - 4- Fluoroaniline 14. 2,5- Dichloroaniline 4.2-Chloroaniline 7. 4- Bromoaniline 15. 2,6- Dichloroaniline 5.2,6-Dimethylaniline 8. 2- Cl - 2- methylaniline 16. 2,6- Diethylaniline 6.3-Cl-4-Fluoroaniline 7.4-Bromoaniline 8.2-Cl-2-methylaniline 9.3-Cl-4-methylaniline 10.2-Ethyl-6-methylaniline 11.3,4-Dichloroaniline 12.4-Isopropylaniline 13.2,4-Dichloroaniline Sample: Anilines Column: ZORBAX SB - C18, 3 x 250 mm, 5 m (P/N: 880975-302) 14.2,5-Dichloroaniline Mobile phase: A = 2.0 mM Potassium Acetate, pH 6.5, B = ACN 15.2,6-Dichloroaniline Gradient: t=0 10% B, hold 2 min. 16.2,6-Diethylaniline
t=2 10% to 45% B over 68 min. t = 70 45% B, hold 15 min. t = 85 45% to 90% B over 1 min. t = 86 90% to 95% B over 7 min 0.35 mL/min Ambient UV 254 nm, ref 460 nm 1. 3- Cl - 4- methoxyaniline 9. 3- Cl - 4- methylaniline
DNPH - Derivatized Aldehydes ZORBAX ODS, 4.6 x 250 mm, 5 m, (P/N: 884950-543) A = 100% Water, B = 100% ACN 60- 75%B in 30 min; Wash: From 75 - 100%B in 5 min, after 5 min return to 60% B 1.0 mL/min 35C UV 230 nm
Flow rate: Temperature: Detection:
Anilines, Substituted: Substituted: Rapid Separation Anilines, Rapid Separation
Anilines, Substituted: pH Effects

3 1. 2. 3. 4. 5. 6. p- Anisidine m- Toluidine 3- Amino - benzonitrile p- Chloroaniline m- Chloroaniline o- Chloroaniline
LC and LC/MS
Anilines,Substituted: Rapid Separation 1.p-Anisidine 2.m-Toluidine 3.3-Amino-benzonitrile 4.p-Chloroaniline 5.m-Chloroaniline 6.o-Chloroaniline
1. 2. 3. 4. 5. 6.
p- Anisidine m- Toluidine 3- Amino - benzonitrile p- Chloroaniline m- Chloroaniline o- Chloroaniline
Anilines, Substituted: pH Effects pH 2.5 1.p-Anisidine 2.m-Toluidine 3.3-Amino-benzonitrile 4 4.p-Chloroaniline 5 1 2 5.m-Chloroaniline 6.o-Chloroaniline
0 1 2 3 4 2 pH 7.0 1 3 4 5 5 6 7
6 8 9 min.
6 0 Instrument: Sample: Column: Mobile phase: Flow rate: Temperature: Detection: Agilent 1050 Anilines ZORBAX SB - C8, 4.6 x 75 mm, 3.5 m, (P/N: 866953- 906) 20% ACN/80% 25 mM phosphate buffer, pH 2.5 3.0 mL/min 60C UV 254 nm 1 2 3 4 5 6 7 8 9 min.
Anilines ZORBAX SB - C18, 4.6 x 150 mm, 5 m, (P/N: 883975- 902) 42% methanol/58% 25 mM phosphate buffer 1.0 mL/min 22C UV 254 nm
622

Environmental
Anions with Agilent HPLC Ion-Kit
Catechol and Various Metabolites Separated on a Wide-Pore Column
1. 2. 3. 4.
Catechol Anthranilic acid 2,3 Dihydroxybiphenyl diol Carbazole
Catechol and Various Metabolites Separated on aWide-Pore Column 1.Catechol 2.Anthranilic acid 3.2,3 Dihydroxybiphenyl diol 4.Carbazole
Anions Asahipak ODP- 50, 4 x 125 mm, 5 m, (P/N: 79923OP-564) Water/acetonitrile, 86/14% + modifier (P/N: 5062-2480) adjusted to pH 8.6 with carbonate free NaOH 1.5 mL/min 40C Explosives 266 nm
Explosives
5 ng 2,4-DNB
(EPA 1.Octogen 2.Nitroglycol 3.Hexogen 4.Nitrodiglycol 5.1,3,5-Trinitrobenzene 6.1,3-Dinitrobenzene 7.Nitroglycerine Explosives (EPA 8330) using Hypersil BDS 8.Tetryl 1. Octogen 10. 2- Amino4,6 - Dinitrotoluene 9.2,4,6 Trinitrotoluene 2. Nitroglycol 11. 4- Amino2,6 - Dinitrotoluene 10.2-Amino4,6-Dinitrotoluene 3. Hexogen 12. 2,4- Dinitrotoluene 13. 2,6- Dinitrotoluene 4. Nitrodiglycol 11.4-Amino2,6-Dinitrotoluene 14. 2- Nitrotoluene 12.2,4-Dinitrotoluene5. 1,3,5-Trinitrobenzene 6. 1,3- Dinitrobenzene 15. 4- Nitrotoluene 13.2,6-Dinitrotoluene 214,1 nm 7. Nitroglycerine 16. 3- Nitrotoluene 14.2-Nitrotoluene 8. Tetryl 17. Nitropenta 15.4-Nitrotoluene 9. 2,4,6 Trinitrotoluene 18. Diphenylamine 16.3-Nitrotoluene 17.Nitropenta 235,4 nm 18.Diphenylamine
5 20 1 3 2 4 6 15 91011 12 16 14 17 13 78
Sample: 15 L, 250 - 500 g/mL each compound Column: ZORBAX 300SB- C18, 4.6 x 150 mm, 5 m, (P/N: 883995- 902) 2O with 0.1% TFA to pH 3 with TEA Mobile phase: A=H 8330) using Hypersil BDS B = ACN with 0.085% TF A Gradient: 5- 60% in 20 min., Wash, 95%B for 2 min. Flow rate: 1.5 mL/min Temperature: 60C Detection: UV 233 nm
LC and LC/MS
NB
3 ng 1,3,5-TNB 5 ng 1-A-6-NT 1,2-DNB 5 ng 1,3-DNB 4 ng 2-A-4,6-NT
6 ng Tetryl 10 ng TNT 5 ng
6 ng 2,4,6-TNP
10 ng Octogen
5 ng Hexogen
1,2-DNB
2-A-4,6-NT 11 ng
6 ng 2-NT 5 ng 4-NT 4 ng 3-NT
12 ng Nitropenta
2,6-DNB
18
DAD1 B, Sig. = 215, 20 nm Ref = 350, 50 nm
10 Time (min)
15
Sample: Column: Mobile phase: Gradient:
Explosive Compounds Hypersil BDS- C18, 4 x 100 mm, 3 m, (P/N: 79926OB-354) A = Water, B = Methanol (MeOH) at 0.0 min 26% B at 10 min 40% B at 20 min 55% B at 30 min 70% B at 31 min 26% B 0.72 mL/min 38C 214 nm and 235 nm
DAD1 A, Sig = 235, 20 nm Ref = 400, 80 nm
10
15
20
25
30
35
40 min.
Trace Level Explosives Hypersil BDS - C18, 2 x 125 mm, 3 m, (P/N: 79926BD - 362) A = Water, B = Methanol 0.5 mL/min 38C UV 214+235 nm, see above
623

Environmental
Explosives and Quantitative Analysis Explosives and and Related RelatedCompounds: Compounds:Qualitative Qualitative and Quantitative Analysis
0.040 0.036 0.032 0.028 0.024 AU 0.020 0.016 0.012 0.008 0.004 0.000 10 Sample: Column: Mobile phase: Gradient: Flow rate: Temperature: Detection: 1 5 2 3 4 6
Explosives and SB Related Qualitative ZORBAX - C18 Compounds: ZORBAX SB - CN and Quantitative Analysis 1.Picric Gradient: acid 0.36 Gradient: 17 2.4-Amino-2-nitrotoluene 0 min. 80%B 0 min. 80%B 17 2 min. 80%B 0.32 3.2-Amino-6-nitrotoluene 1 min. 80%B 7 15 min. 70%B 4.RDX 10 min. 70%B 20 min. 65%B 0.28 30 min. 20%B 8 10 5.2-Amino-4-nitrotoluene 11+12 25 min. 60%B 35 min. 20%B 6.HMX 35 min. 30%B 0.24 37 min. 80%B 16 7.1,3-Dinitrobenzene 40 min. 30%B 42 min. 80%B 0.20 8.1,3,5-Trinitrobenzene 9.2-Amino-4,6-dinitrotoluene 16 9 0.16 10 7 10.2,4-Dinitrotoluene 13+9 8 13 0.12 11.4-Amino-2,6-dinitrotoluene 19 18 5 11 2 3 12.2-Nitrotoluene 0.08 18 4 14+15 6 15 13.2,6-Dinitrotoluene 1 14 0.04 14.4-Nitrotoluene 12 15.3-Nitrotoluene 16.2,4,6-Trinitrotoluene 0.00 17.Tetryl 30 40 min. 10 20 30 min. 20 18.Diphenylamine 10 L of 19 nitromethanes in ACN/H2O (20/80) 19.Hexyl ZORBAX SB - C18, SB - CN, 2.1 x 150 mm, 5 m, (P/N: 883700- 922, 883700 - 905)
A = ACN + 5% H2O + 5 mM CF3COONH4 B = H2O + 5% ACN + 5 mM CF3COONH4, pH 2.7 (CF3COOH) as indicated above 0.23 mL/min 18C UV 210, 240, 360 nm, wavelength switching for each compound AU
1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. 18. 19.
Picric acid 4- Amino - 2- nitrotoluene 2- Amino - 6- nitrotoluene RDX 2- Amino - 4- nitrotoluene HMX 1,3- Dinitrobenzene 1,3,5- Trinitrobenzene 2- Amino - 4,6- dinitrotoluene 2,4- Dinitrotoluene 4- Amino - 2,6- dinitrotoluene 2- Nitrotoluene 2,6- Dinitrotoluene 4- Nitrotoluene 3- Nitrotoluene 2,4,6- Trinitrotoluene Tetryl Diphenylamine Hexyl
LC and LC/MS
Explosives from Soil Extract

Explosives from Soil Extract 1.Octogen (HMX) 2.Hexogen (RDX) 3.2-Amino-6-nitrotoluene 4 4.1,3,5-Trinitrobenzene 5.2-Amino-4-nitrotoluene 1 6.1,3-Dinitrobenzene 8 3 7.Tetryl 8.2,4,6-Trinitrotoluene 5 6 9 9.4-Amino-2,6-dinitrotoluene 10 7 10.2-Amino-4,6-dinitrotoluene 2 11.2,6-Dinitrotoluene 12.2,4-Dinitrotoluene 11 12 13.2-Nitrotoluene 14.4-Nitrotoluene 15.3-Nitrotoluene
0 5 10 15 20 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. Octogen (HMX) Hexogen (RDX) 2- Amino - 6- nitrotoluene 1,3,5- Trinitrobenzene 2- Amino - 4- nitrotoluene 1,3- Dinitrobenzene Tetryl 2,4,6- Trinitrotoluene 4- Amino - 2,6- dinitrotoluene 2- Amino - 4,6- dinitrotoluene 2,6- Dinitrotoluene 2,4- Dinitrotoluene 2- Nitrotoluene 4- Nitrotoluene 3- Nitrotoluene
Herbicides on Different Bonded Phases

Herbicides on Different Bonded Phases 6. Diuron 1. Bentazon 1.Bentazon 2. Tebuthiuron 7. Propazine 3. Simazine 8. Propanil 2.Tebuthiuron 4. Atrazine 9. Prometryne 3.Simazine 5. Prometron 10. Metolachlor 4.Atrazine 5.Prometron 6.Diuron 7.Propazine SB - CN (P/N: 883975 - 905) 8.Propanil 9.Prometryne 10.Metolachlor
SB - PHENYL (P/N: 883975-912)
13
14 15
SB - C8 (P/N: 883975-906)
25
30 min.
10
15
20
25
30
35
40 min.
10 L Explosives Mix ZORBAX SB - C18, 3 x 250 mm, 5 m, (P/N: 880975 - 302) Methanol/Water (50/50) (v/v) 0.3 mL/min Ambient UV 230 nm
Herbicides ZORBAX SB - CN, SB - Phenyl, SB- C8, 4.6 x 150 mm, 5 m, (P/N: see above) 35% ACN, 65% Water 1.0 mL/min Ambient UV 254 nm
624

Environmental
Herbicide/Pesticide Standards: Effect of Bonded Phase
1. 2. 3. 4. 5. Desethyldesisopropylatrazine 6. Simazine Desethylatrazine Methabenzthiazuron Herbicide/Pesticide 7. Standards: Effect of Benzthiazuron 8. Simazine 1.Desethyldesisopropylatrazine Hexazinon 9. Atrazine 2.Desethylatrazine 10. Isoproturon Metoxuron 11. Diuron 12. Monlinuron Bonded Phase 13. Metobromuron 14. Metazachlor 15. Propazine 16. 17. 18. 19. Sebutylazine Terbutylazine Linuron Metolachlor
3.Benzthiazuron 4.Hexazinon 5.Metoxuron 6.Simazine 7.Methabenzthiazuron 8.Simazine 9.Atrazine 10.Isoproturon 11.Diuron 12.Monlinuron 13.Metobromuron 14.Metazachlor 15.Propazine 16.Sebutylazine 17.Terbutylazine Herbicide & Pesticide Standards 18.Linuron ZORBAX Eclipse XDB - C8, 4.6 x 150 mm, 5 m, 19.Metolachlor (P/N: 993967- 906)
Water/Acetonitrile 20- 60% in 15' As indicated As indicated Diode Array, 240/30
Herbicide & Pesticide Standards ZORBAX Eclipse XDB - C18, 4.6 x 150 mm, 5 m, (P/N: 993967- 902) Water/Acetonitrile 20- 60% in 15' As indicated As indicated Diode Array, 240/30
Herbicides: Effect of Column Temperature

A 40 C 1. 2. 3. 4. 5. Simazine Diuron Propazine Prometryn Metolachlor
Herbicides: Use of Short, Small - Particle Columns for Fast and Efficient Separation
2. Prometon Herbicides: Use of 0.3 Short, Small-Particle Columns for Fast and Efficient Separation 4 3. Prometryne 1.Tebuthiuron 8 4. Atrazine 0.2 2.Prometon 5. Bentazon 2 6. Propanil 3.Prometryne 0.1 1 3 6 7. Propazine 7 4.Atrazine 8. Metolachlor 5.Bentazon 0.0 0 5 10 15 min. 6.Propanil B 0.4 7.Propazine 4 5 8.Metolachlor 8 Relative Absorbance Relative Absorbance 0.3 0.2 0.1 2 3 1 6 7 A 1. Tebuthiuron 5
LC and LC/MS
3 1 2
4 5 40 45
Herbicides: Effect of Column Temperature 1.Simazine 2.Diuron 0 5 10 15 20 25 30 35 3.Propazine 4.Prometryn B 4 3 5.Metolachlor 90 C

1 2 5
0 Sample: Column: Mobile phase: Flow rate: Temperature: Detection:
10
15
20 Sample: Column:
0.0 0 5 10 15 20 25 30 min. 0.5 g each A = ZORBAX SB- C8, 4.6 x 75 mm, 3.5 m, (P/N: 866953- 906) B = ZORBAX SB- C8, 4.6 x 150 mm, 5 m, (P/N: 883975- 906) A = Water to pH 2.0 with TFA; B = ACN 2.0 mL/min, 1.0 mL/min 35C UV 254 nm
Herbicides ZORBAX SB - C18, 4.6 x 150 mm, 5 m, (P/N: 883975- 902) 52/48 0.1 M Sodium Acetate/Methanol, pH 6 0.45 mL/min 40C, 90 C UV
Mobile phase: Flow rate: Temperature: Detection:
625

Environmental
PAHs Standard and Soil Sample Overlay
Sample: Column: Mobile Phase: Overlay Gradient: Flow Rate: Temperature: Detection: 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. Standard and soil sample overlay Vydac C18, 2.1 x 250 mm, 5 m, (P/N: 79918PAH-582) Channel A = Water, Channel B = Acetonitrile 0.0 - 3 min %B = 60, 10.5 min %B = 90, 20 min %B = 100, 22.5 min %B = 100, 25 min %B = 60 0.44 mL/min 28C Diode array (254/4, 270/4 nm) and Fluorescence in series
Scaled 90 80 70 60 50 40 30 20 10 0 5
2 3 4
PAHsStandard and Soil Sample 1.Phenanthrene 5 7 2.Anthracene 3.Fluoranthene 4.Pyrene 5.Chrysene 6.Benzo(b)fluoranthene 7.Benzo(k)fluoranthene 8 8.Benzo(a)pyrene 9.Benzo(g,h,i)perylene 6 10.Indeno(1,2,3,-cd)pyrene 11.Coronene
Phenanthrene Anthracene Fluoranthene Pyrene Chrysene Benzo(b)fluoranthene Benzo(k)fluoranthene Benzo(a)pyrene Benzo(g,h,i)perylene Indeno(1,2,3, - cd)pyrene Coronene
10
11 Standard
Sample
10 Time (min)
15
20
25
30
LC and LC/MS
PAHs Comparison of Standard and Narrow Bore

1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. Naphthalene Acenaphthalene PAHs-Comparison of Standard and Fluorene 1.Naphthalene %F Phenanthrene 2.Acenaphthalene 140 Anthracene 3.Fluorene Fluoranthene 120 4.Phenanthrene Pyrene 5.Anthracene Benzo(a)anthracene 100 Chrysene 6.Fluoranthene Benzo(e)pyrene 7.Pyrene 80 1 Benzo(b)fluoranthene 8.Benzo(a)anthracene 60 Benzo(k)fluoranthene Benzo(a)pyrene 9.Chrysene 40 10.Benzo(e)pyrene Dibenzo(a,h)anthracene Benzo(g,h,i)perylene 11.Benzo(b)fluoranthene 20 Indeno(1,2,3,- cd)pyrene 12.Benzo(k)fluoranthene 0 13.Benzo(a)pyrene 14.Dibenzo(a,h)anthracene 15.Benzo(g,h,i)perylene 16.Indeno(1,2,3,-cd)pyrene Sample: Column: Mobile Phase: Gradient: Flow Rate: Temperature: Detection:
Narrow Bore
2 5 7 12 13 4 9 3 6 8 10 11 14 15 16 B
10 Time (min)
15
20
16 PNAs (PAHs) listed by the US EPA A = Vydac C18, 4.6 x 250 mm, 5 m, (P/N: 79918PAH - 584) B = Vydac C18, 2.1 x 250 mm, 5 m, (P/N: 79918PAH - 582) A = Water, B = Acetonitrile 0.0 - 2.5 min %B = 60, 12 min %B = 90, 20 min %B = 100, 22.5 min %B = 100, 25 min %B = 60 (a) 1.5 mL/min, (b) 0.42 mL/min 28C Diode array (254/4, 270/4 nm) and Fluorescence in series, fluorescence shown
626

Environmental
PAHs on 3 mm Columns
1. Napthalene PAHs on 3 mm Columns 9. 2. Acenaphtalene 10. 1.Napthalene 3. Fluorene 11. 2.Acenaphtalene 4. Phenanthrene 12. 3.Fluorene 5. Anthracene 13. 4.Phenanthrene 6. Fluoranthene 14. 7. Pyrene 5.Anthracene 15. 8. Benzo(a)anthracene 6.Fluoranthene 7.Pyrene 8.Benzo(a)anthracene 9.Chrysene 10.Benzo(a)fluoranthene 11.Benzo(k)fluoranthene 12.Benzo(a)pyrene 13.Dibenzo(a,h)anthracene 14.Benzo(g,h,i)perylene 15.Indeno(1,2,3-cd)pyrene Chrysene Benzo(a)fluoranthene Benzo(k)fluoranthene Benzo(a)pyrene Dibenzo(a,h)anthracene Benzo(g,h,i)perylene Indeno(1,2,3-cd)pyrene 200 mAU 300 Diquat Paraquat
Paraquat in Water
Paraquat in Water Diquat Paraquat
100 256 nm (offset) 0 310 nm 1 2 3 Time [min] 4 5
Sample: Column: Mobile phase: Flow rate: Detection:
PAH Standard LiChrospher PAH, 3.0 X 250 mm, 5 m, (P/N: 79925PA - 583) A = Water, B = Acetonitrile 0 min 50% B, 3 min 60% B, 15.4 min 100% B, 23.5 min 50% B 0.8 mL/min 27C 254 nm
Paraquat and Diquat, 10 L/injection Hypersil ODS, 2.1 x 100 mm, 5 m, (P/N: 79916OD - 552) 0.1% hexanesulfonic acid 0.35% triethylamine pH 2.5 (H3P04) 0.4 mL/min 256/10 nm and 310/10 nm (reference 450/100 nm)
Herbicides: Rapid Separation

Herbicides: Rapid Separation 1.Tebuthiuron 2.Atrazine 3.Diuron 4.Propazine 1 5.Propanil 3 5 6.Prometryne
1. 2. 3. 4. 5. 6. Tebuthiuron Atrazine Diuron Propazine Propanil Prometryne
Triazine Pesticides on Bonus-RP and Alkyl C8 Phase
LC and LC/MS
Triazine Pesticides on Bonus-RP and Alkyl C8 Phase 1.Prometryne 2.Tebuthion Bonus - RP 3.Atrazine 4.Propazine 5.Diuron 6.Propanil 7.Dacthal
6 2 4
ZORBAX Eclipse XDB C18 Alkyl C8
HPLC System: Column: Mobile Phase: Detector: Flow: Temperature: Lab Insight:
Agilent 1100 with quaternary pump ZORBAX Eclipse XDB - C18 Rapid - Resolution (3.5 m) Cartridge - Column, 4.6 x 30 mm (P/N: 931975-932) MeOH: water (60:40) UV 254 nm with standard flow cell (13 L) 2 mL/ min Ambient EN9
Instrument: Columns: Mobile Phase: Flow Rate: Temperature: Injection Vol: Detection
Agilent 1100 4.6 x 150 mm (P/N: 883668 - 901) MeOH: 0.1% TFA (70:30)* 1.0 mL/min Ambient 2 L 254 nm
Sample: 1. Prometryne 2. Tebuthion 3. Atrazine 4. Propazine
5. Diuron 6. Propanil 7. Dacthal
* For low pH work with BonusRP, a TFA mobile phase is often preferred over phosphate, and is compatible with LC/MS.
627

Environmental
Pesticides Analysis of Pesticides in Drinking Water
Sample: Column: Mobile phase: Gradient: Flow rate: Temperature: Detection: Pesticides ZORBAX SB - C18, 3.0 x 250 mm, 5 m, (P/N: 880975 - 302) A = 2 mM Sodium Acetate (pH 6.5) with 5% ACN B = 100% Acetonitrile (ACN) 2 min, 10% B; 10 to 45% B in 70 min. 0.35 mL/min 40C UV 245 nm
Standard (500ng/L) 10 8 mAU 13 6 4 2 0 10 20 30 40 1 4 2 5 7 3 89 6
15 25 18 17 19 27 21 20 23 28 24 26
14 10 11 12 16 22
50
60
70 min.
Pesticides using LiChrospher Phase 1.DIPA 2.Metamitron 3.Cloridazon 4.DEA5.Crimidin Pesticides using LiChrospher Phase 6.Carbetamide 7.Bromacil 8.Simazine 50.0 9.Cyanazine 10.DETBA 11.Karbutyl 1 14 12.Methabenzthiazuron 25 20 21 13.Chlorotoluron4 19 24 8 18 9 14.Atrazine mV 3 10 12 15.Monolinuron 16.Diuron 2 13 22 16 17.Metabromuron 23 15 26 6 7 11 18.Dimefuron 17 5 19.Sebutylazine 0.0 20.Propazine 21.Terbutylazine 22.Linuron 2.5 23.Chloroxuron 0 10 20 30 40 50 24.Prometryn Time (min) 25.Terbutryn 26.Metalacher Sample: Pesticides Standards 27.Pencycuron Column: LiChrospher 100 RP - 18, 3.0 x 250 mm, 5 m, (P/N: 7992518 - 583) 28.Bifenox Mobile phase: A = 1 mM Ammonium acetate, B = Acetonitrile 29.Pendimenthaline Gradient: 0-10 min. 25% B, 70 min. 70% B, 90 - 100 min. 90% B, 101 min. 25% B
Flow rate: Temperature: Detection: 0.4 mL/min 28C UV 220 nm
LC and LC/MS
Pesticides Analysis of Pesticides in Drinking Water 16. 2. Metamitron 1.Desisopropylatrazine 3. Fenuron 17. 2.Metamitron 18. 4. Chloridazon 19. 5. Desethylatrazine 3.Fenuron 20. 6. Metoxuron 4.Chloridazon 21. 7. Carbetamid 5.Desethylatrazine 22. 8. Bromacil 6.Metoxuron 23. 9. Hexazinon 7.Carbetamid 24. 10. Simazine 25. 11. Metribuzin 8.Bromacil 26. 12. Desethylterbutylazine 9.Hexazinon 27. 13. Carbutilat 10.Simazine 28. 14. Methabenzthiazuron 11.Metribuzin 12.Desethylterbutylazine Surface Water Sample 13.Carbutilat 15 14.Methabenzthiazuron 12 15.Chlortoluron 10 16.Atrazine 17.Monolinuron 8 18.Diuron 6 19.Isoproturon 4 20.Metobromuron 19 21.Metazachlor 2 22.Buturon 10 20 30 40 50 60 23.Propazine 24.Dimefuron 25.Terbuthylazine 26.Linuron 27.Chlorbromuron 28.Chloroxuron
mAU
1. Desisopropylatrazine
15. Chlortoluron Atrazine Monolinuron Diuron Isoproturon Metobromuron Metazachlor Buturon Propazine Dimefuron Terbuthylazine Linuron Chlorbromuron Chloroxuron
70
80 min.
27 28
29
60
70
80
90
1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. 18. 19. 20. 21. 22. 23. 24. 25. 26. 27. 28. 29.
DIPA Metamitron Cloridazon DEA Crimidin Carbetamide Bromacil Simazine Cyanazine DETBA Karbutyl Methabenzthiazuron Chlorotoluron Atrazine Monolinuron Diuron Metabromuron Dimefuron Sebutylazine Propazine Terbutylazine Linuron Chloroxuron Prometryn Terbutryn Metalacher Pencycuron Bifenox Pendimenthaline
628

Environmental
Pesticides in Surface Water
1. 2. 3. 4. 5. 6. Metamitron Simazine Atrazine Diuron Isoproturon Etofumesate
Phenols, Substituted
Phenols, Substituted 2. 4- Nitrophenol 9. 4- Chloro - 3- methylphenol 3. m- Cresol 10. 2,4- Dichlorophenol 1.Phenol 4. o- Cresol 11. 2- Methyl- 4,6- dinitrophenol 2.4-Nitrophenol 5. 2- Chlorophenol 12. 2,4,6- Trichlorophenol 3.m-Cresol 6. 2,4- Dinitrophenol 13. 2,3,4,6- Tetrachlorophenol 4.o-Cresol 7. 2- Nitrophenol 14. Pentachlorophenol 5.2-Chlorophenol 6.2,4-Dinitrophenol 14 11 6 7.2-Nitrophenol 13 8.2,4-Nimethylphenol 2 9.4-Chloro-3-methylphenol 10.2,4-Dichlorophenol 12 11.2-Methyl-4,6-dinitrophenol 7 10 12.2,4,6-Trichlorophenol 9 13.2,3,4,6-Tetrachlorophenol 14.Pentachlorophenol
8 1 3 5 4 5 6 7 8 9 10 11 12 13 min. 1. Phenol 8. 2,4- Nimethylphenol
mAU 80 70 60 50 40 30 20 10 0 0.0
Pesticides in Surface Water 1.Metamitron 2.Simazine 4 3.Atrazine 4.Diuron 5.Isoproturon 6.Etofumesate 1

2 10 Time (min.) 3 5 20
6 30
Surface Water Hypersil ODS, 2.1 x 100 mm, 5 m, (P/N: 79916OD - 552) Mobile Phase: Water/Acetonitrile Flow Rate: 0.21 mL/min Temperature: 30 C Detection: 214 nm
Sample: Column:
Phenols ZORBAX SB - C18, 4.6 x 150 mm, 5 m, (P/N: 883975 - 902) 20% ACN/80% 0.01 M H3 PO4 to 45% ACN in 7.5 min. 80% ACN in 2.0 min. 1.5 mL/min 35C UV 254 nm
Phenoxycarboxylic Acids
Phenoxycarboxylic Acids 1.DICAMBA 2.2,4-D 3.MCPA 4.2,4-DP 5.MCPP 6.2,4,5-T 7.2,4,5-TP 8.2,4-DB 9.MCPB
1. 2. 3. 4. 5. 6. 7. 8. 9. DICAMBA 2,4- D MCPA 2,4- DP MCPP 2,4,5- T 2,4,5- TP 2,4- DB MCPB
Plant Hormones: Rapid Gradient Elution Separation
LC and LC/MS
Plant Hormones: Rapid Gradient Elution 2. n- 6- Benzyl adenine 3. 3- Indole acetic acid Separation 4. 1- Naphthyl acetamide 1.Kinetin 5. 3- Indole proprionic acid 2.n-6-Benzyl adenine 6. o- Chlorophenoxy acetic acid 3.3-Indole acetic acid 7. p- Chlorophenoxy acetic acid 4.1-Naphthyl acetamide 8. 3-Inodole butyric acid 1 5.3-Indole proprionic acid 9. 1-Naphthyl acetic acid o- Chlorophenoxy proprionic acid 6.o-Chlorophenoxy acetic10. acid 2 3,4,5- Trichlorophenoxy acetic acid 7.p-Chlorophenoxy acetic 11. acid 12. 3,4,5- Trichlorophenoxy proprionic acid 8.3-Inodole butyric acid 3 acetic acid 9.1-Naphthyl 9 5 10.o-Chlorophenoxy 4 proprionic acid 11.3,4,5-Trichlorophenoxy acetic acid 8 12.3,4,5-Trichlorophenoxy proprionic acid 11
6 7 10 12
1. Kinetin
Phenoxycarboxylic acids ZORBAX SB-C18, 4.6 x 250 mm, 5 m (P/N: 880975 - 902) A = 20mM KH2PO4 , B = ACN t = 0 min, 10% B for 2 min t = 2 min, 10% to 65% B over 23 min t = 25 min, 65% B 1.0 mL/min 40C UV 245 nm
2 Time (min)
Plant hormones ZORBAX SB-C18, 4.6 x 75 mm, 3.5 m, (P/N: 886953 - 906) A = water with 0.1% TFA B = Acetonitrile with 0.1% TF A 3.0 mL/min 60C UV 254 nm
629

Environmental
Sulfonylurea Pesticides: High Resolution Separation
1. CL 9673 metabolite of pyridate Sulfonylurea Pesticides: High Resolution Separation 2. Thifensulfuron - methyl 1.CL 9673 metabolite of pyridate 3. Metsulfuron- methyl 2.Thifensulfuron-methyl 4. Chlorsulfuron - methyl 3.Metsulfuron-methyl 5. Rimsulfuron - methyl 6. Tribenuron - methyl 4.Chlorsulfuron-methyl 7. Bensulfuron - methyl 5.Rimsulfuron-methyl 6.Tribenuron-methyl 4 7.Bensulfuron-methyl 2 3 1 905C 5
Triazines: Effect of Temperature

Triazines: Effect of Temperature 1.Simazine 2.Atrazine 3.Promaton 505C 4.Ametryn 5.Propazine 6.Prometryn 7.Terbutryn
1. 2. 3. 4. 5. 6. 7. Simazine Atrazine Promaton Ametryn Propazine Prometryn Terbutryn
30
40 Time (min)
50
60 Sample: Column: Mobile phase: Instrument: Flow rate: Temperature: Detection: 5 g each; 5 L, Triazine Mix ZORBAX SB - C18, 4.6 x 150 mm, 5 m, (P/N: 883975 - 902) 33/67 Acetonitrile/0.1 Sodium acetate buffer, pH 6.0 HP - 1050 1.5 mL/min A: 50C; B: 90C UV 210 nm
Sulfonylurea Pesticides ZORBAX SB - C18, 3.0 x 250 mm, 5 m, (P/N: 880975 - 302) A = 0.01% Acetic Acid in Water, B = Acetonitrile, 0.01% Acetic Acid 0.5 mL/min 40C UV 230, 270 nm
Volatiles Air Analysis, LC
LC and LC/MS
2 5 3 1 0 2 4 4
6 Volatiles Air 1.DNPH (reagent) 2.Aldol 8 3.Formaldehyde 9 10 4.Acetaldehyde 5.Propionaldehyde 12 6.Crotonaldehyde 7 7.Butyraldehyde 11 8.Benazldehyde 9.Valeraldehyde 10.Caproicaldehyde 11.Heptaldehyde 6 8 10 12.Citronellal 13.Capricaldehyde
1. DNPH (reagent) 2. Aldol 3. Formaldehyde 4. Acetaldehyde 5. Propionaldehyde 6. Crotonaldehyde 7. Butyraldehyde Analysis, LC
8. 9. 10. 11. 12. 13.
Benazldehyde Valeraldehyde Caproicaldehyde Heptaldehyde Citronellal Capricaldehyde
13
Sample: Column: Mobile phase: Gradient: Flow rate: Temperature: Detection: 12 min.
Volatile Aldehyde Compounds, 20 L/injection Nucleosil 100 - 5 C18, 4.0 x 250 mm, 5 m, (P/N: 7992718- 584) A = Acetonitrile, B = Water From 27% B to 100% B in 13 min. 0.45 mL/min 35C UV 365/30 nm, ref 590/10 nm
630

Food and Consumer Products

Aflatoxins: Comparison of Diode Array with Fluorescence Detection
25 20 15 10 5 0 0
Antioxidants
Sample: Column: Mobile phase: Gradient: Antioxidants Standard, 2 L/injection Hypersil BDS - C18, 4 x 125 mm, 5 m (P/N: 79926OB-564) A = water + 0.2 mL H2SO4 to pH 2.3 B = Acetonitrile start with 10 %B at 3 min 60 % B at 4 min 80 % B at 6 min 90 % B at 7 min 10 % B 2.0 mL/min 1 min 40 C UV - DAD, 260/80 nm spectral information and retention times
G 2 1.5 ng M1 10 ng FLD: ex 365 nm M 2 5 ng em 455 nm DAD: 365 nm 2 4 Time (min)
B 21.5 ng
G 15 ng
B 1 5 ng
10
Flow rate: Post time: Temperature: Detection: Confirmation: mAU 20 15 10 5 0 2
Aflatoxin Standard Hypersil ODS, 2.1 x 100 mm, 3 m, (P/N: 79916OD-352) Isocratic Water/Methanol/ Acetonitrile (63/26/11) mixture 0.30 mL/min 25C Fluorescence ex 365 nm, em 455 nm Diode - array 365 nm
THBP
PG
BHA Standard BHT TBHQ 3 4 Time [min] 5
LC and LC/MS
Anthocyanins from Blueberries: High-Efficiency High-Speed Separation
Natural Anthocyanins ZORBAX SB - C18, 4.6 x 250 mm, 5 m, 4.6 x 150 mm, 3.5 m, 4.6 x 75 mm, 3.5 m, (P/N: 880975- 902, 863953- 902, 866953- 902, respectively) A = 3% Phosphoric acid, B = 100% MeOH as shown 1.0 mL/min 30C UV 525 nm
631

Antioxidants in Food, High-Speed Hypersil ODS
Sample: Antioxidants in Food, 2 L/injection -344) Column: Antioxidants Hypersil ODS, 4.6 x 60 mm, 3 m, (P/N: 79916OD in Food, High-Speed Hypersil ODS Mobile Phases: A = H20 with 0.005 M NH4 Ac, pH to 4.2 1.Benzoic acid B = Acetonitrile 2.Sorbic C =acid H 20 + 0.2 mL H2 S04 3.PHB-Methyl Gradient program for A and B (f. preservatives): at 3 min %B = 60 at 4 min %B = 80 4.PHB-Ethyl at 5 min %B = 80 at 6 min %B = 10 5.PHB-Propyl 6.BHAat 7 in %B = 10 Gradient program for C and B (f. antioxidants): 7.BHTat 3 min %B = 60 at 4 min %B = 80 at 5 min %B = 90 at 6 min %B = 10 at 7 in %B = 10 Flow Rate: 4 mL/min 23 4 5 Temperature: 40C Standard Detection: 260/80 nm 1 6 7 1. Benzoic acid 2. Sorbic acid White Wine 3. PHB- Methyl 4. PHB- Ethyl 5. PHB- Propyl Deodorant 6. BHA 7. BHT Salad Dressing
Aromatics
Aromatic Sample 1. Acetophenone 1 2. Cinnamaldehyde 3. Eugenol 4. Cinnamaldehyde Impurity Aromatics 5. Ethyl cinnamate 1.Acetophenone 6. p-Cymene
2.Cinnamaldehyde 3.Eugenol 4.Cinnamaldehyde Impurity 5.Ethyl cinnamate 2 6.p-Cymene5

3 4 6
2 3 Time (min)
Column: Mobile Phase: UV: Flow: Temperature: Lab Insight:
ZORBAX Eclipse XDB-Phenyl, 4.6 x 150 mm, 3.5 m (P/N: 963967-912) H 2O : MeOH, 40:60 254 nm 1.0 mL/min 35C FD13
Aspartame: Metabolites and Applications
B Vitamins
LC and LC/MS
Aspartame: 1.Phenylalanine 2.5-benzyl-3, 6-dioxo-2-piperazineacetic acid 3.Aspartic acid-phenylalanine dipeptide Diet Coke 4.Aspartame 1. Caffeine 5.Diet Coke 2. Aspartame 6.Equal Sweetener 3. Unknown
Aspartame and Its Metabolites 1. Phenylalanine 2. 5- benzyl - 3, 6 - dioxo - 2- piperazineacetic acid 3. Aspartic acid - phenylalanine dipeptide 4. Aspartameand Applications Metabolites
B Vitamins 1.Vitamin B1, Thiaminium Dichloride 2.Vitamin B6, Pyridoxol Hydrochloride 3.Vitamin B12, Cyanocobalamin
2
1. Vitamin B1, Thiaminium Dichloride 2. Vitamin B6, Pyridoxol Hydrochloride 3. Vitamin B12, Cyanocobalamin
Equal Sweetener 1. Aspartame

0.0 8.0 12.0 Time (min) 18.0 24.0 30.0
Aspartame ZORBAX SB-C18, 4.6 x 75 mm, 3.5 m, (P/N: 866953-902) 85/15, 0.1% TFA/ACN 1.0 mL/min 35C UV 210 nm
Vitamin B Complex Purospher RP - 18, 4 x 125 mm, 5 m, (P/N: 79925PU - 564) 70/30, 0.04 M Phosphate buffer pH 3.5/Methanol 1.0 mL/min Ambient 254 nm
632

Carbohydrates: Carbohydrate Standards
Carbohydrates: Separation Showing High Sensitivity Sensitivity of High Injection Volume (50 L)
10 ng limit of detection
Carbohydrates: Carbohydrate Standards 1.Ribose 2.Rhamnose 3.XKylose 4.Fructose 5.Glucose 6.Sucrose 25 ng total in 1 L injection volume 7.Maltose 8.Lactose 9.Raffinose
Instrument: Column: Mobile Phase: Flow Rate: Injection: Agilent 1100 RID ZORBAX Carbohydrate Analysis Column, 4.6 x 150 mm (P/N: 843300-908) 63% CH 3CN/H2) 0.5 mL/min. A: 25 ng/ L, 1 L injected B: 500 pg/ L, 50 L injected Sample: 1. Ribose 2. Rhamnose 3. XKylose 4. Fructose
25 ng total in 50 L injection volume
5. 6. 7. 8. 9.
Glucose Sucrose Maltose Lactose Raffinose
Bitter Compounds, Hesperidine, Naringenine

mAU 20 15 Hesperidine 10 5 Orange juice 0 -5 Naringenine Standard
Flavoring Agents
LC and LC/MS
Bitter Compounds, Hesperidine, Naringenine Orange juice Hesperidine 0.5 1 1.5 2 Naringenine
Time [min]
Cool Mint Listerine Sample 1. Unknown 2. Benzoic acid 3. Methyl salicylate 4. Carvone 5. Unknown 6. Thymol 7. Anethole
2.5
Flow rate: Temperature: Post time: Detection: Confirmation:
Orange Juice, 1 mL/injection Hypersil BDS - C18, 4 x 125 mm, 5 m, (P/N: 79926OB - 564) A = Water and 0.15 mL/L H 2SO4 (conc.), pH 2.4 B = Acetonitrile start with 20% B at 3 min 20% B at 5 min 90% B at 6 min 20% B 2 mL/min 40C 1 min UV - DAD, 260/80 nm spectral information and retention times
Flavoring Agents Cool Mint Listerine Benzoic acid Methyl salicylate Carvone 4 Thymol Anethole
3 5
Column: Mobile Phase: UV: Flow: Temperature:
ZORBAX SB Phenyl Narrow-Bore LC/MS, 2.1 x 50 mm 5 m (P/N: 860975-912) 0.3% TF A : ACN, 65:35 254 nm 0.3 mL /min. Ambient
633

Flavors
1. Vanillin alcohol 2. 4- Hydroxy benzoic acid Flavors 3. Vanillin 1.Vanillin alcohol 4. 4- Hydroxy benzaldehyde 2.4-Hydroxy benzoic acid 5. Ethyl - vanillin 3.Vanillin 6. Coumarin 4.4-Hydroxy benzaldehyde 5.Ethyl-vanillin 6.Coumarin Vanillin extract 100 Standard 0.5 1 1 2 5 3 4 1.5 Time [min] 2 2.5 5 3 6
Food Colors, FD&C

1. 2. 3. 4. 5. Yellow # 5 Red # 40 Blue # 1 Propylparaben Red # 3 C16H9N4Na3O9S2 C18H14N2Na2O8S2 C37H34N2Na2O9S3 C10H12O3 C20H4I4Na2O5 MW= 534 MW= 496 MW= 760 MW= 180 MW= 878
mAU 300
200
Food Colors, FD&C 1 1.Yellow #5 2.Red #40 3.Blue #1 4.Propylparaben 5.Red #3
ZORBAX Eclipse XDB - C18 2
Sample: Column: Mobile phase: Gradient: Flow rate: Temperature: Post time: Detection: Confirmation:
Vanilla Extract, 1 L/injection Hypersil BDS - C18, 4 x 125 mm, 5 m, (P/N: 79926OB - 564) A = water + 0.15 mL/L H 2 SO4 (conc.), pH 2.3 B = acetonitrile start with 5% B, at 10 min 60% B 2.0 mL/min 40C 1 min UV - DAD, 260/80 nm spectral information and retention times
Operating Conditions: HPLC System: Agilent 1100 with quaternary pump Column: ZORBAX Eclipse XDB - C18 Rapid - Resolution (3.5 m), 4.6 x 50 mm P/N: 93596-7902 Mobile Phase: A: 0.1% TF A, pH to 4.4 with TEA, B: MeOH Gradient: 17 to 100% B/4 min Detector: UV 254 nm Flow: 1 mL/min Temperature: Ambient Pub: LI FD16
LC and LC/MS
Colors, Synthetic: Comparison of Different Mobile Phase and Detection Parameters

Sample: Synthetic Food Colors, 1 L/injection Column: Hypersil BDS - C18, 3 x 125 mm, 3 m, (P/N: 79926BD - 363) Mobile phase A: a = 0.01 M NaH 2 PO4 + 0.001 M Tetrabutylammonium dihydrogenphosphate pH 4.2 b = acetonitrile (ACN) Gradient for A: start with 15% b, in 10 min to 40% b, in 14 min to 90% b, until 19 min at 90% b, in 20 min to 15% b. Mobile phase B: a = 0.01 M NaH2PO4 + 0.001 M Tetrabutyl ammoniumhydrogen sulfate pH 4.8 b = acetonitrile (ACN) start with 15% b, in 10 min to 40% b, in 14 min to 90% b, until 19 min at 90% b, in 20 min to 15% b. Mobile phase C: a = 0.01 M ammoniumacetate pH = 4.9, b = ACN Gradient for C: start with 7% b, in 10 min to 40% b, in 14 min to 90% b, until 19 min at 90% b, in 20 min to 7% b. phase D:Mobile a = 0.01 M NaHand pH = 4.3, 2P0 4 , Colors, Synthetic: Comparison Mobile of Different Phase Detection b = acetronitrile (ACN) E102 Tartrazine Gradient for D: start with 5% b, E123 Amaranth in 10 min to 60% b, E124 Ponceau 4R in 14 min to 90% b, 3 E110 Sunset until 19 min at 90% b, 3 yellow in 20 min to 5% b. E125 Scarlet red 2 2 E127 Erythrosine 5 5 5 41 4 6 6 6 Peak 5 6 A B C D 14 min. 1. 2. 3. 4. 5. 6. Gradient for B: Stop time: Post time: Flow rate: Temperature: Detection: 20 min 4 min 0.8 mL/min 40C A: 254 nm/50 nm B: 350 nm/20 nm C: 465 nm/30 nm D: 600 nm/40 nm
Parameters
mAU 1000 3 800 600 400 200 1 0 2 4 6 1 2 3
Compound Number of name sulfonic groups E102 T artrazine 2 E123 Amaranth 3 E124 Ponceau 4R 3 E110 Sunset yellow 2 E125 Scarlet red none E127 Erythrosine none
10
12
634

Hydroperoxides in Aged Oils
Sample: Aged Sunflower Oil, 1 L/injection Column: Hypersil MOS, 2.1 x 200 mm, 5 m, (P/N: 79916MO- 572) Mobile phase: A = Water B = Acetonitrile/Methyl- tert.butylether 9:1 Hydroperoxides in Aged Oils Gradient: at 0 min 87% B 1. Aged Sunflower Oil at 25 min 100% B Flow Rate: 0.8 mL/min Temperature: 60C Post time: 4 min Detection: UV, 200 and 215 nm for triglycerides, 240 nm for hydroperoxides, 280 nm for tocopherols and decomposed triglycerides Confirmation: Evaluation of spectra was performed to trace hydroperoxides and to differentiate saturated and different unsaturated triglycerides
Neutraceuticals: Extract from Green Tea

1. 2. 3. 4. 5. Epigallocatechin Epicatechin Epigallocatechin gallate Catechol Epicatechin gallate Green Tea Extract 2
Catechin Mixture
Neutraceuticals: Extract from Green Tea 2 1.Epigallocatechin 2.Epicatechin3 .Epigallocatechin gallate 3 4 4.Catechol 5.Epicatechin gallate
1 1 5
OLL
OOL
Aged sunflower oil * Hydroperoxides * Absorbance (scaled) LLL 120
unknown 4 3 5
80 * * 40 * 0 5 10 15 * *
PLL OOO SOO 215 nm 240 nm 20 25 min.
Column: Mobile Phase: Inj. Vol. Temperature: Detection:
ZORBAX SB - C8, 4.6 x 150 mm; 3.5 m (P/N: 863953- 906) 75% 0.1% Trifluoroacetic acid: 25% Methanol 5 L, 1 mL/min 40 C Det. UV (280 nm)
Polysaccharide Size Separation
Carbohydrates: Effect of Mobile Phase Strength
LC and LC/MS
ACN/H2O: 70/30 Mono- disaccharides 1. Fructose 2. Glucose
Carbohydrates: Effect of Mobile Phase Strength 3. Saccharose 1.Fructose 4. Palatinose 2.Glucose 5. Trehalulose 6. Isomaltose 3.Saccharose 4.Palatinose 4 5.Trehalulose 6.Isomaltose
ACN/H2O: 75/25
1. MW = 40,000 polysaccharide (Rheomacrodex), 2. Glucose ZORBAX Bimodal Kit, Unsilanized, 2 columns, 6.2 x 250 mm, 5 m, (P/N: 880949- 903) 8.5 g NaCl per liter water 2.0 mL/min 35C UV 205 nm and RI
Mono - and Disaccarides ZORBAX NH 2 , 4.6 x 250 mm, 5 m, (P/N: 880952- 708) ACN/Water, as indicated 1.0 mL/min Ambient RI
635

Sweeteners, Artificial
Sample: Column: Mobile phase: Gradient: Aspartame Standard Derivatized with o - phthalaldehyde (OPA), mercapto - propionic acid (MP A) agents, 1 L/injection Hypersil ODS, 2.1 x 100 mm, 5 m, (P/N: 79916OD -552) A = 0.01 mM sodium- acetate, B = methanol start with 5% B at 5 min 25% B at 10 min 35% B at 13 min 55% B at 18 min 80% B at 20 min 95% B 0.35 mL/min 40C 5 min UV - DAD, 338/20 nm or fluorescence ex 230, em 445 nm UV - Vis spectral information and retention times
Tocopherols: Analysis of Margarine with Fluorescence Detection

1. 2. 3. 4. alpha Tocopherol beta Tocopherol gamma Tocopherol delta Tocopherol
Flow rate: Temperature: Post time: Detection: Confirmation: 70
%F Tocopherols: Analysis of Margarine with Fluorescence Detection 2 (77.3%) 100 1.alpha Tocopherol 3 90 2.beta Tocopherol 3.gamma Tocopherol 80 4.delta Tocopherol 70 60 50 40 30 20 10 1 (11.2%) 1 2 3 1 2 3 (9.5%) 4 (1.9%) 4 5 Standard Margarine 6 min. 4
Aspartame spectra scaled original derivatized
60 Absorbance [mAU] 50 40 30 20 10 0 0 2 4
Aspartame
250 300 350 Wavelength (nm) Standard

6 8 10
Margarine LiChrospher 100 Diol, 4 x 125 mm, 5 m, (P/N: 79925DI - 564) Hexane + 2% Isopropanol 1.0 mL/min 25C Fluorescent, Ex = 295, Em = 330
Time [min]
Carbohydrates in Colas
Carbohydrates: Sugar Alcohols

Fructose Glucose Chewing gum, sugar- free
LC and LC/MS
COLA, Fountain
Carbohydrates in Colas COLA, Can, Brand A 1. Cola
Carbohydrates: Sugar Alcohols Sorbitol 1. Chewing gum, sugar-free Xylitol 2. Xylitol Unknown 3. Sorbitol
Xylitol COLA, Brand B Sorbitol
COLA, Brand B, diet
Instrument: Column: Mobile Phase: Temperature: Flow Rate: Sample:
Agilent 1100 RID ZORBAX Carbohydrate Analysis Column, 4.6 x 150 mm (P/N: 843300-908) 75% ACN 25% H 2 0 30C 2.0 mL/min No dilution
Instrument: Column: Mobile Phase: Temperature: Flow Rate:
Agilent 1100 RID ZORBAX Carbohydrate Analysis Column, 4.6 x 150 mm (P/N: 843300-908) 75% ACN 25% H 2 0 30C 2.0 mL/min
636

Carbohydrates in Juices and Milk
A. Apple Drink 36.8% Fructose 24.9% Sucrose 38.3% Glucose Lactose B. Milk (2%)
Apple Juice Fructose
58.7% Fructose 9.9% Sucrose 33.4% Glucose Glucose Sucrose
100% Lactose - Free Milk
Carbohydrates in Juices and Milk 1. Apple Drink 2. Milk (2%) 3. Apple Juice 4. 100% Lactose-Free Milk
Glucose
Galactose
Agilent 1100 RID ZORBAX Carbohydrate Analysis Column, 4.6 x 150 mm (P/N: 843300-908) 75% ACN/25% H20 30C 2.0 mL/min Diluted to 0.1X in 50:50 ACN:H2O
Agilent 1100 RID ZORBAX Carbohydrate Analysis Column, 4.6 x 150 mm (P/N: 843300-908) 75% ACN/25% H20 30C 2.0 mL/min Partitioned between MeCl 2: H2O
Carbohydrates: Syrups and Honey

Corn Syrup 1 : 50 dilution Raffinose
Carbohydrates: Yogurts
Fruit Bar
LC and LC/MS
Carbohydrates: Syrups and Honey 1. Corn Syrup 2. Pancake Syrup 3. Honey 4. Molasses 5. Caramel Syrup
Pancake Syrup 1 : 100 dilution Raffinose Honey 1 : 200 dilution
Plain Yogurt
Blueberry, Fat - Free Yogurt
Instrument: Column: Mobile Phase: Temperature: Flow Rate:
Carbohydrates: Yogurts 1. Fruit Bar 2. Plain Yogurt Caramel Syrup 3. Blueberry ,Fat-Free Yogurt 1 : 10 dilution 4. Blueberry Yogurt 5. Raffinose 6. Sucrose 7. Lactose Agilent 1100 RID 0 8.843300-908) Maltose ZORBAX Carbohydrate Analysis Column, 4.6 x 150 mm (P/N: 9. Glucose/Galactose 75% ACN/25% H20 Instrument: 30C Column: 10. Fructose
2.0 mL/min Mobile Phase: Temperature: Flow Rate:
Molasses 1 : 50 dilution
Blueberry Yogurt
Sucrose Glucose/Galactose Fructose 1 2 3 Maltose Lactose Raffinose
min
Agilent 1100 RID ZORBAX Carbohydrate Analysis Column, 4.6 x 150 mm (P/N: 843300-908) 75% ACN/25% H20 30C 2.0 mL/min
637

Colorants
E102=Tatrazine E123=Amaranth E110=Yellow No. 6 E124=Ponceau 4R E125=Scarlet Red E127=Erytrosine E125
Parabens: High Speed Separation

1. 2. 3. 4. Methylparaben Ethylparaben Propylparaben Butylparaben
Standard mAU 200 150 100 50 0 2 Sample: Column: Mobile phase: Gradient: Flow rate: Temperature: Post time: Detection: Confirmation: E102 E123
E127
Colorants E124 E110 Sig=254,50 Ref=550,80 Tatrazine Amaranthellow No. 6 Ponceau 4R Sig=520,20 Ref=off Scarlet Red Erytrosine
Parabens: High Speed Separation ZORBAX SB - C18 1 1.Methylparaben 2 2.Ethylparaben 3 Ambient 3.Propylparaben 4 4.Butylparaben
Sig=450,20 Ref=580,40 60 C
min.
Mixture of Food Dyes, 1 L/injection Hypersil BDS - C18, 4 x 125 mm, 5 m, (P/N: 79926OB - 564) A = water and 0.1 M NaH2 PO4, pH=4.6 B = acetonitrile start with 5% B at 10 min 60% B 0.8 mL/min 40C 4 min UV - DAD, 254, 450, 520 nm, as shown spectral information and retention times
HPLC System: Column: Mobile Phase: Detector: Flow: Inj. Volume: Temperature:
Agilent 1100 with quaternary pump ZORBAX StableBond - C18 Rapid - Resolution (3.5 m) Cartridge - Column, 4.6 x 30 mm (P/N 833975 - 902) 0.1% H3PO4: ACN, (50:50) UV 254 nm with standard flow cell (13 L) 2 mL/ min 1L top: ambient, bottom: 60 C
LC and LC/MS
Fatty Acids
mAU C 18-3 1500 C 18-2 C 18-1
1000
Standard
500 C6 0 C8 C 10 C 12 C14 C16 C18
Rape oil C 20 C 22
Standard 15 Sample: Column: Mobile phase: Gradient: 20 25 30 min. Injector program for online derivatization 1 DRA W:2.0 mL from: air 2 DRA W:3.0 mL from: vial 10 acetonitrile 3 DRA W:0.0 mL from: vial 4 wash bottle (ACN) 4 DRA W:0.7 mL from: vial 6 deriv. agent 5 DRA W:0.0 mL from: vial 4 wash bottle (ACN) 6 DRA W:0.5 mL from: sample 7 DRA W:0.0 mL from: vial 4 wash bottle(ACN) 8 DRA W:0.7 mL from: vial 6 deriv. agent 9 DRA W:0.0 mL from: vial 4 wash bottle(ACN) 10 DRA W:1.0 mL from: vial 10 acetonitrile 11 MIX: 8 mL cycles: 10 12 WAIT 1.0 min 13 INJECT
Flow rate: Post time: Stop time: Temperature: Detection:
Standards and Rape Oil derivatized with 150 mg bromo - phenacyl bromide dissolved in 4 mL acetonitrile Hypersil BDS - C18, 4 x 100 mm, 3 m, (P/N: 79926OB - 354) A = Water 70%, B = Acetonitrile 30% at 5 min 30% B at 15 min 70% B at 17 min 70% B at 25 min 98% B 0.8 mL/min 4 min 32 min 50C UV, 258 nm with 20 nm bandwidth
638

Ochratoxin: Analysis in Wheat Flour, Analysis with Derivatization in Fig Extract
Neat Sample Ochratoxin A 15.5 ng/20g Sample: Column: Mobile phase: Flow rate: Temperature: Detection: Ochratoxin in Wheat Flour & Fig Extract (as indicated) Lichrospher 100 RP18, 4 x 125 mm, 5 m, (P/N: 79925OD - 564-3) Water with 2% acetic acid/acetonitrile, 1 : 1 1.0 mL/min 40C Fluorescence ex 247 nm, ex 480 nm
Ochratoxin: 1. Ochratoxin 2. Fig Extract

0 2 4 Time [min] Standard Containing Ochratoxin A and Derivatized Ochratoxin A Ochratoxin A Ochratoxin A (derivatized methyl ester) Fig extract Standard 2 4 6 8 10 Time [min] 12 14 2 4 6 8 10 Time [min] 12 14 Derivatized Sample 6 8 Wheat flour Analysis in Wheat Standard
Flour, Analysis with Derivatization in Fig Extract
10
Ochratoxin A (derivatized methyl ester) 25.77ng/mL
LC and LC/MS
Patuline in Apple Juice

Sample: Column: Mobile Phase: Flow rate: Temperature: Detection: Confirmation: Apple Juice LiChrospher Diol, 4 x 125 mm, 5 m, (P/N: 79925DI - 564) Hexane- isopropanol 95:5 as isocratic mixture 0.6 mL/min 30C DAD 270/20 nm Spectral data
30
Patuline in Apple Juice 1. Apple Juice

mAU
20
7 6 5 4 3 2 1 0
Library match 985
Scaled
Patuline 250 300 Wavelength [nm] 350
HMF 10 Patuline
Apple juice 0 2 4 6 Time (min) 8 10 12 Standard 14
639

Preservatives
Sample: Column: Mobile phase: Gradient: Perservatives in food and beverage, 2 L/injection Hypersil ODS, 4.6 x 60 mm, 3 m, (P/N: 79916OD - 344) A = 0.005 M NH4 Ac, pH to 4.2 B = Acetonitrile Preservatives start with 10% B at 4 min 80% B Bencoic acid at 5 min 80% B Sorbic acid at 6 min 10% B PHB-methyl at 7 min 10% B 4 mL/min PHB-ethyl 40C PHB-propyl 1 min BHA UV - DAD, 260/80 nm BHT spectral information 60 and retention times 50 Absorbance [mAU] 40 30 20 White wine 10 Salad dressing 0 0 1 2 Time [min] 3 4 Bencoic acid
Absorbance (scaled)
50 library 30 sample 10 200 240
spectral library match 999
Flow rate: Temperature: Post time: Detection: Confirmation:
280
320
Sorbic acid
Wavelength [nm]
PHB-methyl PHB-ethyl PHB-propyl BHA BHT
Standard
LC and LC/MS
Pesticides in Salad
mAU 120 80
Carbendazim Folpet 3 different extracted salad samples Vinclozolin
Tocopherols: Normal Phase Columns with Diode Array and Fluorescence Detection
40 0
10 15 20 25 30 35 40 100 80 60 40 20 0 0.5
1. 2. 3. 4.
alpha Tocopherol beta Tocopherol gamma Tocopherol delta Tocopherol
Low Levels 1. 15 ng 2. 3.4 ng 3. 97 ng 4. 62 ng
3 20 Columns 17.5 15 12.5 10 7.5 5 2.5 0 4.5
Pesticides in Salad Time [min] Carbendazim Vinclozolin Pesticides extracted from Salads, 3 - 10 L/injection Hypersil BDS - C18, 3 x 125 mm, 3 m, (P/N: 79926BD - 363) Folpet
95/5 Water/Acetonitrile(ACN) at 10 min 25% ACN at 26 min 42% ACN at 34 min 60% ACN at 45 min 100% ACN at 50 min 100% ACN at 55 min 5% ACN 0.5 mL/min 42C 6 min UV 214 nm with 20 nm bandwidth
Tocopherols: Normal Phase Fluorescence Detection 4 1.alpha Tocopherol 2.beta Tocopherol 1 2 3.gamma Tocopherol 4.delta Tocopherol 1 2 3 4
1.5 2.5 min. 3.5
with Diode Arrayand

4 1 2 3 4 FLD DAD 7
1 2 1
2 3 4 5 6 min.
Flow Rate: Temperature: Post time: Detection:
Tocopherols Hypersil Si 100, 2.1 x 100 mm, 5 m, (P/N: 79916SI - -552) Hexane + 2% Isopropanol 0.3 mL/min 25C Fluorescent, Ex = 295, Em = 330
640

Vitamin A and Beta Carotene Fat-Soluble Vitamins: Separation of Vitamin D2 from D3
all- Trans retinol all- Trans acetate (+)- d- Tocopherol Vitamin D2 Vitamin D3 (+)- a- Tocopherol (V itamin E) (+)- a- Tocopherol Acetate Vitamin K1 ZORBAX ODS, 4.6 x 250 mm, 5 m, (P/N: 884950- 543) (non - endcapped) 4 5 12 Fat-Soluble Vitamins: Separation of Vitamin D2 from 8 1.all-Trans retinol 3 6 7 1. 2. 3. 4. 5. 6. 7. 8.
1. Vitamin A - palmitate, 420 ng 2. - carotene, 290 ng 3. - carotene, 27 ng
Scaled 800 700 600 500 400 300 200 100 0 0
Vitamin A and Beta Carotene 1. Vitamin A-palmitate 1 2. -carotene 2 3. -carotene
D3
Standard 3 Fruit Juice (2.7 mg/100 mL) 20 25
10 15 Time [min]
2.all-Trans acetate 3.(+)-d-Tocopherol 4.Vitamin D25.Vitamin D3 6.(+)-a-Tocopherol 4 (Vitamin E) 5 ZORBAX ODS Classic, 4.6 x 150 mm, 5 m, 7.(+)-a-Tocopherol Acetate 1 2 (P/N: 883952- 702) (non - endcapped) 8.Vitamin K1 8
3 0 2.5 5 7.5 6 10 7 12.5 15 17.5 min.
-carotene in fruit juice Lichrospher RP 18, 4 x 125 mm, 5 m (P/N: 79925OD - 5643) Methanol + 5 g/L Lithiumperchlorate + 1 g/L Acetic Acid 1.5 mL/min 30C Amperometric, 1 V (range 0.5 A, ref. AgCl/KCl)
5 L (10 g/L), Fat Soluble Vitamin Standard see above 75/25 ACN/MeOH 1.0 mL/min 40C UV 325 nm for 4 min, 280 nm after
Fat-Soluble Vitamins on ZORBAX Eclipse XDB-C8

1. Retinol Fat-Soluble Vitamins on ZORBAX Eclipse XDB-C8 2. Retinol acetate 1.Retinol 3. Vitamin D3 2.Retinol acetate 4. g- Tocopherol 3.Vitamin D3 5. a- Tocopherol 2 6. Tocopherol acetate 4.g-Tocopherol 7. Retinol palmitate 5.a-Tocopherol 6.Tocopherol acetate 5 2 Ambient 50C 7.Retinol palmitate 1 6 5 6 3 4 7 3 4 7
Water-Soluble Vitamins
LC and LC/MS
Water-Soluble Vitamins 1.B1- Thiamine 2.Vitamin C 3.B3- Niacin 4.B6- Pyridoxine 5.Pantothenic acid 6.Folic acid 7.B12- Cyanocobalamin 8.B2- Riboflavin
1. 2. 3. 4. 5. 6. 7. 8.
B1- Thiamine Vitamin C B3 - Niacin B6 - Pyridoxine Pantothenic acid Folic acid B12- Cyanocobalamin B2 - Riboflavin
10 15 20 25 Time (min)
30
10
15 20 25 Time (min)
30
Fat Soluble Vitamins ZORBAX Eclipse XDB- C8, 4.6 x 150 mm, 5 m (P/N: 993967- 906) 5/95 Water/MeOH 1.0 mL/min Ambient, 50C UV 280 nm
Water soluble vitamins ZORBAX SB - C8, 4.6 x 150 mm, 5 m, (P/N: 883975- 906) A = 50 mM Sodium Phosphate, pH 2.5/MeOH (90/10) B = 50 mM Sodium Phosphate, pH 2.5/MeOH (10/90) 0 - 70% B in 18 min. 1.0 mL/min Ambient UV 245 nm
641

Water-Soluble Vitamins: High Speed Separation using Ion-Pairing Chromatography
Water-Soluble Vitamins: 1.Vitamin C 2.B3 - Niacin 3.B6 - Pyridoxine 4.Folic acid 5.B2 - Riboflavin 6.B1 - Thiamine
6 1 4 5 3 1. Vitamin C 2. B3 - Niacin High Separation 3. B6 -Speed Pyridoxine 4. Folic acid 5. B2 - Riboflavin 6. B1 - Thiamine
Water-Soluble Vitamins using the USP 23 Method
using Ion-Pairing Chromatography
1. 2. 3. 4. E
3 - Niacin B6 - Pyridoxine B2 - Riboflavin B1 - Thiamine Excipient
min.
Water soluble vitamins ZORBAX SB -C8, 4.6 x 75 mm, 3.5 m, (P/N: 866953-906) 10 mM Hexane Sulfonate with 0.1% Phosphoric Acid: MeOH (74:26) 1 mL/min Ambient UV 245 nm
Water-Soluble Vitamins using the USP 23 Method 1.3 - Niacin 2.B6 - Pyridoxine Sample: 3.B2 Water soluble vitamins Riboflavin Column: ZORBAX SB -C18, L1 packing, 4.6 x 250 mm, 5 m, 4.B1 Thiamine (P/N: 880975-902) E. Excipient Mobile phase: 7.2 mM Hexane Sulfonate/MeOH/Acetic Acid
Flow rate: Temperature: Detection: (73/27/1) (ratio to 101) 1.0 mL/min 30C UV 280 nm
LC and LC/MS
Water Soluble Vitamins Separated on ZORBAX SB-Aq
1. Thiamine 2. Nicotinic Acid 3. Pyridoxine 4. Niacinamide
Column:
ZORBAX SB-Aq, 4.6 x 150 mm, 5 m (P/N: 883975-914) Mobile Phase: 5% MeOH/95% TFA (0.1%) Flow Rate: 2.0 mL/min Temperature: 35C Detector: UV (254 nm)
642

Pharmaceutical
Pharmaceutical Pharmaceutical
Chromatographic Improvement at High pH using Amines Analgesics: High Speed Separation
1. Acetaminophen (4 -acetamidophenol) 5. Aspirin (acetosalicylic acid) 6. Salicylic acid 2. Caffeine 3. 2-acetamidophenol Analgesics: High Speed Separation 7. Phenacetin (acetophenetidin) 4. Acetanilide 1.Acetaminophen (4-acetamidophenol) ZORBAX SB -C18 7 2.Caffeine 2 3.2-acetamidophenol 4 5 6 Ion -Pair 4.Acetanilide 1 3 Mobile Phase 5.Aspirin (acetosalicylic acid)
pH 6.85 0 1 2 3 4 5 6 7 8 9 10 11
6.Salicylic acid 7.Phenacetin (acetophenetidin)

Volatile Mobile Phase
pH 12 0 Sample: Column: 1 2 3 4 5 6 7 8 9 10 11 Test Mix, 8 components containing nitrogen Asahipak ODP - 50, 4 x 125 mm, 5 m, cartridge column, (P/N: 79923OP -564) A = 0. 05% Ammonium Acetate, pH - see chromatograms B = Acetonitrile C = Methanol Ternary Gradient 75/10/15 to 35/40/25 A/ B/ C in 10 min 1.0 mL/min Ambient UV 254
Mobile Phase:
HPLC System: Detector: Column: Mobile Phase: Flow: Inj. Volume: Temperature:
Gradient: Flow Rate: Temperature: Detection:
Agilent 1100 with quaternary pump UV 275 nm with standard flow cell (13 L) ZORBAX StableBond -C18 Rapid -Resolution (3.5 m) Cartridge -Column, 4.6 x 30 mm P/N: 833975 -902 1 mM octane sulfonic acid, Na salt, pH 2.5, or 1% formic acid: ACN (80:20) 2 mL/min 1 L 70C
Anesthetics, Local: Bonded Phase Selectivity

SB -C18 P/N: 883975-902 1. 2. 3. 4. 5. Procaine Lidocaine d-Cinchonine Butacaine Tetracaine
Antibiotics: High Speed Separation
LC and LC/MS
1. 2. 3. 4.
Ceftazidime Cefotaxime Ciprofloxacin Cefazolin
SB -C8 1.Procaine P/N: 883975-906
Anesthetics, Local: Bonded Phase Selectivity 2.Lidocaine 3.d-Cinchonine 4.Butacaine 5.Tetracaine

SB -Phenyl P/N: 883975-912
Antibiotics: High Speed Separation 1.Ceftazidime 2.Cefotaxime 3.Ciprofloxacin 4.Cefazolin
SB -C3 P/N: 883975-909
SB -CN P/N: 883975-905
10 L, 10 ug/mL ZORBAX SB, 4.6 x 150 mm, 5 m, see above A = 50 mM NaH2PO4 pH 2.5 in 95% H 2O/ 5% ACN B = 50 mM NaH2PO4 pH 2.5 in 47% H 2O/53% ACN 0-100% B in 18.8 min 1.5 mL/min 26C UV 254 nm
1 L containing 0.40, 0.36, 0.10 and 0.37 g ea. of 1-4 resp. ZORBAX SB -C8, 4.6 x 75 mm, 3.5 m, (P/N: 866953 -906) 8.0% acetonitrile/92% 0.1% aqueous TFA 45-70%B in 35 min. 3.0 mL/min 605C UV 260 nm
643

Pharmaceutical
Antifungal Medications
1 1. Clotrimazole 2. Econazole 3. Miconazole 2
Antihistamines
1. 2. 3. 4. Pseudoephedrine Scopolamine Pyrilamine Chlorpheniramine
Antifungal Medications 1.Clotrimazole unknown 2.Econazole 3.Miconazole

3
Antihistamines 1.Pseudoephedrine 2.Scopolamine 3.Pyrilamine 4.Chlorpheniramine
Column: Mobile Phase: Inj. Vol.: Flow Rate: Temperature: Detection: Pub:
ZORBAX Bonus -RP, 4.6 x 150 m, 5 m (P/N: 883668-901) 35% 25 mM NaH2PO4 , Dibasic (pH 6.5 with H 3PO4): 65% ACN 2 L 1 mL/min Ambient UV (220 nm) LI PH46
Antihistamines ZORBAX Rx, SB -C8, 4.6 x 150 mm, 5 m, (P/N: 883967-901, 883975-906) 10% ACN, 90% 50 mM, KH2PO4, pH 2.5 2.0 mL/min 255 C UV 254 nm
LC and LC/MS
Analgesics: Non-steroidal Anti-inflammatory Drugs: Narrow Bore Separation
Aromatic Acids/Benzoic Acids Selectivity Differences

ZORBAX SB -C8 P/N: 880975-906 45% Methanol ZORBAX SB -Phenyl P/N: 880975-912 40% Methanol
Analgesics: Non-steroidal Anti-inflammatory Drugs: Narrow Bore Separation 1.Phenacetin 2.Tolmetin 3.Phenylbutazone 4.Fenoprofen Aromatic Acids/Benzoic AcidsSelectivity Differences 1.Phthalic 2.2-Nitrobenzoic 3.2-Fluorobenzoic 4.2-Chlorobenzoic Sample: Benzoic acids 5.3-Nitrobenzoic Column: ZORBAX SB - C8, SB - CN, SB - Phenyl, Column, 4.6 x 250 mm, 6.3-Fluorobenzoic 5 m, (P/N: 880975 - 906, 880975 - 905, 880975 -912) 7.m-Toluic Mobile phase: 30-45% methanol (above) in 25 mM Na Phosphate, pH 2.5
Flow rate: Detection: Temperature: 1.0 mL/min UV 254 nm 35C
NSAID 1. Phenacetin 2. Tolmetin 3. Phenylbutazone 4. Fenoprofen
pK a 2.2 3.5 4.4 4.5
2 L, 10 ug/mL ZORBAX Eclipse XDB - C8, 2.1 x 150 mm, 5 m (P/N: 993700-906) 50/50, 25 mM Sodium Phosphate (pH 7.0 with Phosphoric Acid), MeOH 0.2 mL/min 35C UV 254 nm
644

Pharmaceutical
Catecholamines/Biogenic Amines: Rapid Separation using Ion Pair Reagents

DOPA - Dihydroxyphenylalanine DHBA - Dihydroxybenzyl amine DOPAC - Dihydroxyphenyl acetic acid NE - Norepinephrine DA - Dopamine HIAA - Hydroxyindoleacetic acid EP - Epinephrine AVA - Homovanillic acid 5HT - Hydroxytryptamine 3MT - Methaxytyrosine
Catecholamines/Biogenic Amines: Rapid Separation using Ion Pair Reagents Dihydroxyphenylalanine Dihydroxybenzyl amine Dihydroxyphenyl acetic acid Norepinephrine Dopamine Sample: 10 g/mL each standard; volume 20 L (2 g tissue sample) Hydroxyindoleacetic acid Column: ZORBAX SB -C8 Rapid Resolution, Epinephrine 4.6 x 75 mm, 3.5 m, Homovanillic acid (P/N: 866953-906) Hydroxytryptamine Mobile Phase: 0.14 M sodium phosphate, Methaxytyrosine 20 mM EDT A,
Flow rate: Temperature: Detection: 0.75 mM octyl sulfonate, 9% methanol pH 3.5 1.5 mL/min 26C 0.75 V vs Ag/AgCI with electrochemical detection
LC and LC/MS
Chiral Separation of D, L Methadone and D,L Norgestral

Methadone Methadone ChiraDex, 4 x 250 mm, 5 m, (P/N: 79925CB -584) Mobile phase: Triethylammonium acetate solution (1%) pH 4.1/acetonitrile, 98/2 Flow rate: 0.8 mL/min Detection: UV 254 nm 0 4 Sample: Column: O C H3 CH 3 CH 3 CH 3
Chiral Ethiazide (Diuretic Drug) Separation on Ultron ES-OVM
12 8 RT (min)
16
20
Norgestral Norgestral ChiraDex, 4 x 250 mm, 5 m, (P/N: 79925CB -584) Mobile phase: Methanol/Water 20/80 Flow rate: 1.0 mL/min Temperature: Ambient Detection: UV 220 nm 0 5 10 15 RT (min) 20 Sample: Column:
20 L containing 0.35 g Ethiazide Ultron ES -OVM, 4.6 x 150 mm, 5 m, (P/N: 702111651) 20 mM KH 2PO 4 (pH 4.6) 1.0 mL/min 25C UV 220 nm
645

Pharmaceutical
Chiral Separation of Fluoxetine Enantioners (Prozac) using Ultron ES-OVM Chiral Separation of Hexobarbitol
O N N O
Chiral Separation of Fluoxetine Enantioners (Prozac) using Ultron ES-OVM Fluoxetine 1. Fluoxetine
12
24
36 RT (min)
48
Mixture Fluoxetine (Prozac) enantiomers ULTRON ES -OVM, 4.6 x 150 mm, 5 m, (P/N: 702111651) 25/75 (v/v) EtOH / 20 mM KH2 PO 4 , pH 5.5 (adjusted with NaOH) 0.8 mL/min Ambient UV 225 nm
Sample: Hexobarbitol Chiral Separation Hexobarbitol Column: ChiraDex, 4of x 250 mm, 5 m, (P/N: 79925CB-584) 1. Hexobarbitol Mobile phase: Methanol/water, 20:80 Flow rate: 1.0 mL/min Detection: UV 220 nm
Courtesy of D.S. Ristry and V.S. Sharp, Eli Lilly and Co.
LC and LC/MS
Chiral Separation of S- and R - Norfluoxetine using Ultron ES -Pepsin
Chiral Separation of Salbutamol on Ultron ES- Pepsin
Chiral Separation of Salbutamol on Ultron ES-Pepsin 1. Salbutamol Chiral Separation of S- and R-Norfluoxetine using Ultron ES-Pepsin 1. Norfluoxetine
50 g/mL of 2:3 mixture R - : S -Norfluoxetine ULTRON ES -Pepsin, 4.6 x 250 mm, 10 m, (P/N: 724111653) 6/94 (v/v) MeOH / 20 mM KH2 PO4 1.0 mL/min Ambient UV 225 nm
Courtesy of D.S. Ristry and V.S. Sharp, Eli Lilly and Co.
20 L containing 0.35 g Salbutamol Mixture Ultron ES -Pepsin, 4.6 x 150 mm, 5 m, (P/N: 822111631) 20 mM phosphate buffer, pH 6.0 1.0 mL/min 25C UV 220 nm
646

Pharmaceutical
Chiral Separation of Tolperison Enantiomers on Ultron ES-OVM Chiral Separation of Atenolol on Ultron ES - Pepsin
Chiral Separation of Atenolol on Ultron ES-Pepsin Atenolol Racemic
Chiral Separation of Tolperison Enantiomers on Ultron ES-OVM Tolperison

Sample: Column: Mobile Phase: Flow rate: Temperature: Detection: Tolperison, 5 L Ultron ES -OVM, 4.6 x 150 mm, 5 m, (P/N: 702111651) 20 mM KH 2PO4 (pH 5.5), C 2H 5OH (100/4 v/v) 1.0 mL/min Ambient UV 220 nm, 0.04 AUFS Sample: Column: Mobile Phase: Flow rate: Temperature: Detection:
1.5 L, 0.25 mg/mL, Atenolol Racemic Mixture Ultron ES - Pepsin, 4.6 x 150 mm, 5 m, (P/N: 82111631A) 20 mM phosphate buffer, pH 6.0/Ethanol (99/1) 1.0 mL/min 25C UV 220 nm, 0.04 AUFS
Cocaine and Metabolites

1. Benzoylecgonine 2. Cocaine 3. Ecgonine methylester
Aspirin and Cough Remedy on ZORBAX Eclipse XDB -C8
LC and LC/MS
1. Acetylsalicylic acid 2. Dextromethorphan t0 1 2 3 1 t0 Cocaine and Metabolites 1. Benzoylecgonine 2. Cocaine 3. Ecgonine methylester 2 80 C 3 ZORBAX Rx -SIL 40 C
Aspirin and Cough Remedy on ZORBAX Eclipse XDB-C8 1. Acetylsalicylic acid 2. Dextromethorphan
HPLC System: Agilent 1100 with quaternary pump Column: ZORBAX Rx -Sil 4.6 x 150 mm (P/N: 883975-901) Mobile Phase: MeOH: NH 4 Acetate, 25 mM, pH 6 (70:30) Detection: UV 210 nm Flow: 1 mL/min Temperature: 40 and 80 C Lab Insight: PH42
5 L, 10 ug/mL ZORBAX Eclipse XDB -C8, 4.6 x 150 mm, 5 m (P/N: 993967-906) (75:25) 25 mM Na2 HPO4 (pH 3.0) : ACN 1.0 mL/min 40C UV 254 nm
647

Pharmaceutical
Metronidazole: Updating USP Methods

Plates 9772 ZORBAX -C8 USP TF 1.30 Particle Size 5 m
Morphine and Metabolites: Extracted Blood Plasma Sample Separation

1. Morphine-3-glucuronide 125 ng 2. Morphine-6-glucuronide 62.5 ng 3. Morphine 12.5 ng in Water
ZORBAX Eclipse XDB -C8 Plates USP TF Particle Size 9850 0.98 3.5 m
Metronidazole: Updating USP Methods ZORBAX Eclipse XDB -C8 Rapid Resolution 1. Metronidazole Plates USP TF Particle Size
21554 1.13 3.5 m
Morphine and Metabolites: Extracted Blood Plasma Sample Separation 1. Morphine-3-glucuronide 2. Morphine-6-glucuronide 3 .Morphine
Electrochemical detection 720 mV Sample: Column: Metronidazole A = ZORBAX C8, 4.6 x 150 mm, 5 m, (P/N: 883952 -706) B = ZORBAX Eclipse XDB-C8, 4.6 x 150 mm, 5 m (P/N: 993967-906) C = ZORBAX Eclipse XDB -C8 Rapid Resolution, 4.6 x 150 mm, 3.5 m (P/N: 963967-906) 80/20, Water/Methanol 1.0 mL/min Ambient UV 254 nm
Fluorescence detection exc. = 285 nm, em. = 352 nm
Mobile phase: Flow rate: Temperature: Detection:
50 L, Amounts Indicated Above ZORBAX SB -C18, 4.6 x 150 mm, 3.5 m, (P/N: 863953 -902) 97/3 70 mM KH 2PO4 + 1 mM EDTA/ACN, pH 4.5 1.5 mL/min Ambient Electrochemical/Fluorescence
Courtesy of J. Visser, Center for Pharmacy Univ. Groningen, The Netherlands.
LC and LC/MS
Neutraceuticals: Hypericin Separation in St. John's Wort

Hypericin Standard Extract of St. John' s Wort
Opiate Drugs, Basic Compounds Showing Good Peak Shape

1. Morphine 2. Codeine 3. Oxymorphone
Neutraceuticals: Hypericin Separation in St. John's Wort 1. Hypericin Standard Hypericin 2. Extract of St. John's Wort 3. Hypericin
Opiate Drugs, Basic Compounds Showing Good Peak Shape 1. Morphine 2. Codeine 3. Oxymorphone
Column: Mobile Phase: Inj. Vol.: Flow Rate: Temperature: Detection: Pub:
ZORBAX Eclipse XDB -C8, 4.6 x 150 mm (P/N: 993967-906 ) 23% 25 mM Na2HPO4 , Dibasic (pH 7.0 with H 3PO 4): 77% MeOH 2 L 1 mL/min 35C 254 nm LI PH47
Opiate Drugs ZORBAX Rx/SB-C8, 4.6 x 150 mm, 5 m, (P/N: 883967-901 or 883975-906) 7% ACN / 93% 25 mM KH2PO4, pH 3.5 1.5 mL/min 25C UV 214 nm
648

Pharmaceutical
Procainamides and Metabolites Separated at High pH
1. Tocainamide 2. Procainamide 3. Quinine 4. Disopyramide 5. Dihydroquinine 6. Diphenhydramine Separated at High pH
Oxindole Alkaloids
1. Spectiophyllin 1. 2. Uncarin F 2. 3. Mitraphyllin Mitraphyllin 3. 4. Pteropodin Pteropodin 4. 5. Rhynchophyllin Rhynchophyllin 5. 6. Isorrhynchophyllin 6. Isorrhynchophyllin 7. Isopteropodin 7. Isopteropodin
Procainamides and Metabolites 1. Tocainamide 4 2. Procainamide 3. Quinine 4. Disopyramide 1 3 5. Dihydroquinine 6. Diphenhydramine
5 0 2 4 6 8 10 min.
Oxindole Alkaloids 1. Spectiophyllin 2. Uncarin F 3. Mitraphyllin 4. Pteropodin 5. Rhynchophyllin 6. Isorrhynchophyllin 7. Isopteropodin
Sample: Column: Mobile phase Gradient: Flow rate: Temperature: Detection:
Basic Drugs Asahipak ODP -50, 4 x 250 mm, 5 m (P/N: 79923OP-584) A = Buffer pH 12, 1:19 diluted B = Acetonitrile 1070% B in 8 min 1 mL/min 50C UV
10 L, Oxindole Alkaloid Mix ZORBAX RX -C18, 4.6 x 250 mm, 5 m, (P/N: 880967 -902) A = 10 mM Phosphate Buffer pH 6.6 B = (1:1) MeOH : ACN 45-70%B in 35 min. 1.0 mL/min 15C UV 245 nm
Courtesy of Dr. H. Stuppner, Inst. Pharmacognosy, Univ. Innsbruck, Austria.
Steroids
Steroid Sample 1. Prednisolone 2. Corticosterone 3. 11 -hydroxyprogesterone 11# hydroxyprogesterone 4. Cortisone acetate 5. Deoxycorticosterone 6. 17 -hydroxyprogesterone 17# hydroxyprogesterone 7. Progesterone 1 ZORBAX Eclipse Steroids XDB -Phenyl, 3.5 m 1. Prednisolone (P/N: 963967-912) 22. Corticosterone 3. 11 -hydroxyprogesterone 3 Cortisone acetate 4. 5. Deoxycorticosterone 5 6. 176-hydroxyprogesterone 7.4Progesterone 7 1 2 ZORBAX Eclipse XDB -C18, 5 m (P/N: 993967-902)
Tetracyclines
LC and LC/MS
1. 2. 3. 4. 5.
Oxytetracycline Epi -tetracycline Tetracycline Chlortetracycline Doxycycline Doxycyclinhyclat
3 5 4 6 7
Tetracyclines 1. Oxytetracycline 2. Epi-tetracycline 3. Tetracycline 4. Chlortetracycline 5. Doxycycline
Columns: Mobile Phase: Flow Rate: Temperature: Detect: UV : Pub:
4.6 x 150 mm see part numbers above H 2O : ACN, 60:40 1.0 mL/min 35C 254 nm LI PH36
Tetracyclines Hypersil BDS -C18, 3 x 125 mm, 3 m, (P/N: 79926BD -363) A = Water, pH 2.1 (H 2SO4) B = Acetonitrile 0 min 15%B 90 min 90%B 15 min 60%B 100 min 90%B 70 min 70%B 101 min 25%B 0.3 mL/min 27C UV 350 nm
649

Pharmaceutical
Tetracyclines Tricyclic Antidepressants
1. 2. 3. 4. 5.
Uracil Minocycline Tetracycline Chlortetracycline Doxycycline
Tetracyclines 1. Oxytetracycline 2. Epi-tetracycline 3. Tetracycline 4. Chlortetracycline 5. Doxycycline
1. 2. 3. 4. 5. 6.
Propranolol Nortriptyline Doxepin Doxepin dimer Amitriptyline Trimipramine
Tricyclic Antidepressants 1. Propranolol 2. Nortriptyline 3. Doxepin 4. Doxepin dimer 5. Amitriptyline 6. Trimipramine
Sample: Column: Sample: Column: Mobile phase: Flow rate: Temperature: Detection: Tetracyclines ZORBAX Rx -C8, 4.6 x 150 mm, 5 m, (P/N: 883967-901) 75/25 0.1% TFA/Acetonitrile 1.0 mL/min Ambient UV 254 nm Mobile phase: Flow rate: Temperature: Detection:
10 L, Antidepressant Mix, 10 g/mL ZORBAX Eclipse XDB -C8, 4.6 x 150 mm, 5 m, (P/N: 993967-906) 38/62 THF/25 mM Potassium Phosphate, pH7 1.0 mL/min 23C UV 254 nm
LC and LC/MS
Tricyclic Antidepressants and Metabolites: Effect of Pore Size

1. 2. 3. 4. 5. 6. Trans 10-OH - Nortriptyline Trans 10 -OH - Amitriptyline Cis 10 -OH - Nortriptyline Cis 10 -OH - Amitriptyline Nortriptyline Amitriptyline
Tricyclic Antidepressants, Screening Application at High pH

1. Desipramine 2. Nortriptyline 3. Doxepine 4. Impramine 5. Amitriptyline Application at High pH
SB-C18 (3.5 )
220 200
Tricyclic Antidepressants and Metabolites: Effect ofPore Size 1. Trans 10-OH- Nortriptyline 2. Trans 10-OH- Amitriptyline 3. Cis 10-OH- Nortriptyline 4. Cis 10-OH- Amitriptyline 5. Nortriptyline 6. Amitriptyline
300 SB-C18
Tricyclic Antidepressants, Screening 180 1. Desipramine 160 2 2. Nortriptyline 5 140 3. Doxepine 120 4. Impramine 5. Amitriptyline 100
80 60 40 20 0 1 4
10 L, Antidepressant Mix, 10 g/mL ZORBAX SB -C18, 4.6 x 150 mm, 3.5 m, (P/N: 863953 -902) ZORBAX 300 SB -C18, 4.6 x 150 mm, 5 m, (P/N: 883995-902) 40/60, 25 mM Phosphate Buffer, 10 mM triethylamine, pH6.2/ACN 1.2 mL/min Ambient UV 254 nm
5 min.
Tricyclic Antidepressants Antidepressents Asahipak ODP -50, 4 x 250 mm, 5 m, (P/N: 79923OP -584) Phosphate buffer pH 12, Acetonitrile 1.0 mL/min 50C UV
650

Pharmaceutical
Ulcer Treatment Drugs at Intermediate pH Urine, LSD Analysis by LC/MS
1. Famotidine 2. Cimetidine 3. Pirenzepine
2,3 =1.3 1,2 =1.8

1 2 3 ZORBAX BONUS-RP Zorbax Bonus -RP
Ulcer Treatment Drugs at Intermediate pH 2 1,2 =2.6 1. Famotidine 2. Cimetidine 2,3=1.9 3. Pirenzepine1
Alkyl -C8 3
LSD Analysis by LC/MS Instrument: Urine, Agilent 1100 LC/MSD System Column: 1. ZORBAX LSD Eclipse XDB -C8, 2.1 x 50 mm, 5 m (P/N: 960967-906) Mobile Phase: 15 : 85, ACN : 10 mM Ammonium Formate, pH 3.7 Flow: 0.3 mL/min Inj vol: 30 L Temperature: 30 C Detection: MS Lab Insight: PH35
Agilent 1100 MSD Conditions: SIM mode, Ions: 324.2, 223.1, 208.1 Fragmentor (dynamically ramped) 100V at 324.2, 148V at 223.1, 170V at 208.1
HPLC System: Agilent 1100 with quaternary pump Column: ZORBAX Bonus -RP, 4.6 x 150 mm (P/N: 883668 -901) Mobile Phase: Na citrate, 20 mM, pH 6.1: MeOH, (80:20) Detector: UV 220 nm Flow: 1 mL/min Temperature: ambient
Hughes, J.M., C.A. Miller and S.M. Fischer, "Development of a Method for the Forensic Analysis of LSD in Urine", presented at the ASMS, Palm Springs, June 1997.
USP Method: Glyburide and Internal Standard, Progesterone
Dexamethasone, USP Method: Rapid Analysis
LC and LC/MS
Dexamethasone 1. Glyburide 2. Progesterone
4.6 x 250 mm, 5 m
4.6 x 75 mm, 3.5 m
USP Method: Glyburide and Internal Standard,Progesterone 1. Glyburide 2. Progesterone
Dexamethasone, USP Method: Rapid Analysis 1. Dexamethasone
Time (min)
Time (min)
Time (min) Sample: Column: Mobile phase: Flow rate: Temperature: Detection: 5 L, 10 ug/mL each of standard ZORBAX Eclipse XDB -C8, 4.6 x 250 mm, 5 m (P/N: 990967-906) 45/55, 50 mM Ammonium Phosphate / ACN, Final pH 5.35 1.5 mL/min Ambient UV 254 nm
10 L and 5 L, 10 ug/mL ZORBAX SB -C8, 4.6 x 250 mm, 5 m, 4.6 x 75 mm, 3.5 m, (P/N: 880975-906, 866953-906) A = Water, B = ACN; Isocratic 30% B 2.0 mL/min Ambient UV 254 nm
651

Pharmaceutical
USP Method: Triamcinolone and Internal Standard, Hydrocortisone

1. Triamcinolone 2. Hydrocortisone ZORBAX Rx -C18 ZORBAX SB -C18 Originally specified C18 (3.9 x 300 mm)
USP Method: Triamcinolone and Internal Standard, Hydrocortisone 1. Triamcinolone 2. Hydrocortisone
5 Time (min) Sample: Column: Mobile phase: Flow rate: Temperature: Detection:
10
5 Time (min)
10
5 Time (min)
10
10 L, 10 ug/mL ZORBAX Rx -C18, SB -C18, 4.6 x 250 mm, 5 m (P/N: 880967-902, 880975-902) Water: MeOH, 40/60 1.5 mL/min Ambient UV 254 nm
LC and LC/MS
Hydrophilic Purine/Pyrimidine Separation

1. Cytosine 2. Fluorocytosine 3. Uracil 4. Guanine 5. Thymine 6. Adenine
Column:
ZORBAX SB-Aq, 4.6 x 150 mm, 5 m (P/N: 883975-914) Mobile Phase: 50 mM NaOAc, pH 4.6 Flow Rate: 2.0 mL/min Temperature: 35C Detector: UV (254 nm)
652
General CE Supplies
Reagents
Comprehensive Offering to Support Your CE

The Agilent Technologies Capillary Electrophoresis System is a highly flexible tool for the analysis of a wide range of different compounds. With the introduction of the High Sensitivity Dection Cell, the Agilent CE system improves detection sensitivity an amazing ten-fold over standard capillaries. The result is HPLC-like sensitivity, HPLC-like ruggedness and HPLC-like reproducibility. Which means that the technique of CE is a viable option for a much wider range of applications. Agilent has specific solutions kits, designed for both organic and inorganic analysis. These kits feature ruggedness and repeatability in addition to being extremely easy to use. We also offer a comprehensive selection of buffer formulations conveniently readyto-use right out of the bottle. From fused silica to extended light path to the PVA coated variety. If you've been searching for greater sensitivity, you've found the perfect fit. CE and Agilent.
CE
Capillary Electrophoresis Reagents

Reagents for CE
Premade buffers help eliminate the time-consuming buffer preparation process. All Agilent Technologies buffers and reagents are designed to meet the stringent demands of CE. To maintain high reproducibility, all buffer solutions are standardized to 0.02 pH units under class 10 clean room conditions. Superior quality control ensures reproducible results bottleto-bottle and batch-to-batch.
CE-Grade Water
Description Ultra pure water for CE Volume (mL) 500 Part No. 5062-8578
Capillary Conditioning Solutions

Description 0.1 N sodium hydroxide 1.0 N sodium hydroxide 0.1 N phosphoric acid Volume (mL) 250 250 250 Part No. 5062-8575 5062-8576 5062-8577
CE and CE/MS
653
General CE Supplies
Reagents/Vials and Caps
CZE Buffers for Charged Analytes
Description 50 mM sodium phosphate buffer, pH 2.5 50 mM sodium phosphate buffer, pH 7.0 50 mM sodium tetraborate buffer, pH 9.3 20 mM sodium tetraborate buffer, pH 9.3 Volume(mL) Part No. 250 250 250 100 5062-8571 5062-8572 5062-8573 8500-6782
Buffers for CZE of Proteins

Description 50 mM phosphate, 0.05% hydroxyethyl cellulose buffer, pH 2.5 150 mM phosphate, 200 mM ammonium sulfate buffer, pH 7.0* Volume(mL) Part No. 250 250 8500-6786 8500-6787
*Use 25 m id capillary to limit current generation
Micellar Electrokinetic Chromatography (MEKC) Buffer for Neutral and Charged Analytes
Description 50 mM sodium tetraborate, 100 mM sodium dodecyl sulfate buffer, pH 9.3* Volume(mL) Part No. 250 5062-8574
*Dilute with 50 mM sodium tetraborate, pH 9.3 (P/N 5062-8573) to reduce SDS concentration without affecting the tetraborate composition or pH.
Vials and Caps for CE

Description Vial, polypropylene, 1 mL Clear wide opening crimp/snap top vial, 2 mL Clear wide opening crimp/snap top vial, 2 mL Amber wide opening crimp/snap top vial, write-on spot, 2 mL
Units Part No.
100/pk 100/pk 500/pk 100/pk
5182-0567 5182-9697 5183-4623 5183-4619
CE and CE/MS
Microvials and Caps

12 x 32 mm Microvials Units Part No.
Polypropylene crimp/snap top vial, 300 L Polypropylene glass-lined crimp/snap top vial, 100 L
Vial Caps
1000/pk 100/pk
9301-0978 9301-0977
Snap caps PEO (polyethylene olefin for chemical resistance) Snap caps PEO (polyethylene olefin for chemical resistance) Snap caps PUR (polyurethane for resealing)
100/pk 500/pk 100/pk
5181-1507 5181-1513 5181-1512
Note: The PUR caps are completely sealed to help prevent sample or buffer evaporation even after multiple injections.
654
CE Instrument Parts and Supplies

Cells and Cell Accessories
High Sensitivity Detection Cell

The Agilent high sensitivity detection cella technological leap which extends sensitivity by an order of magnitudeprovides a solution to sensitivity limitations often encountered in CE. This improvement will substantially increase the utility of CE for impurity analysis of chiral drugs, biologicals, and compounds of environmental interest, among others. The high linear range allows quantification of both <0.1% impurities and the main component in one run. This is useful for all impurity determinations and is especially useful for determining chiral excess. The Agilent Technologies high sensitivity detection cell can be used in all Agilent CE systems. Helpful Hint: Increased sensitivity also means increased visibility of impurities. To ensure maximum performance use only buffers and solvents of the highest quality. All Agilent buffers and reagents are of electrophoresis grade to meet your system needs.
High Sensitivity Detection Cell

Description High sensitivity detection cell kit: Detection cell, 75 m id inlet capillary (72 cm) and outlet capillary (8.5 cm) pair, capillary cassette, fittings (3 fitting screws with seals, 2 fitting caps), cleaning solution, CE Partner CD-ROM Replacement capillary kits:* 75 m id capillary pair with 8.5 cm outlet and the following inlets: PVA coated capillaries, 75 m id capillary pair with 8.5 cm outlet and the following inlet: Replacement fittings: 3 fitting screws with seals, 2 fitting caps Replacement detection cell Cell cleaning solution, 1L 56 cm effective length G1600-68716 72 cm effective length G1600-68715 88 cm effective length G1600-68714 56 cm effective length G1600-68319 G1600-63200 G1600-60027 5062-8529
*CEC capillaries are also available for the HSDC (see page 665666).
Specs
Part No. G1600-68713
CE and CE/MS
Helpful Hint: See our listing of CEC Capillaries for a complete selection.
655
CE/MS Instrument Parts and Supplies

Accessories
Instrument Supplies
Description Deuterium lamp Electrode assembly Electrode O-ring, silicone Electrolyte bottle Electrolyte bottle cap Glass frit (Solvent inlet filter) Filter frit adapter, 3 mm Bottle cap plug Air filter, 5 m Pre-puncher Screws for pre-puncher/insulation plate holding 10/pk 4/pk 5/pk 500 mL Unit Part No. 2140-0585 G1600-60007 5062-8544 9300-1748 9300-1747 5041-2168 5062-8517 G1600-23223 3150-0619 G1600-67201 G1600-62402
Accessory Kit
Description Agilent Capillary electrophoresis accessory kit: Includes electrode tool, screwdriver, fuses, air filter, glass frit, vials and caps Optical filters 260-nm diode array filter for DNA analysis with polycyclamide filled capillaries and oligonucleotide analysis Rack, plastic for 12 mm crimp top microvials, holds 50 Agilent CE column cutter Diamond blade replacement kit for CE cutter 5/pk Unit Part No. G1600-68701
G1600-62700 9301-0722 5183-4669 5183-4670
CE and CE/MS
656

Accessories
CE/MS Accessories
The CE/MS adapter kit simplifies coupling the Agilent CE system with MS systems equipped with an electrospray ionization source. Integral to this kit is the CE/MS cassette which completely thermostats the capillary until it exits the CE system. The cassette offers multiple capillary paths which vary the capillary length. A methods development configuration uses on-line diode array detection and MS. For rapid or routine MS analysis the detector can be bypassed to decrease the total capillary length and reduce analysis time. The CE/MS adapter kit can be used with the Agilent 1100 Series MSD, MSD-Trap, or virtually any electospray-MS platform.
Agilent CE-MS Adapter Kit

Description Kit: Use to interface the Agilent CE system with a mass spectrometer. Includes parts below, which may be ordered separately.* CE/MS interface cassette, metallic Nonmetallic alignment interface for 360 m od capillaries CE/MS capillary, 50 m id, L=125 cm, l=21.5 cm 2/pk Unit Part No. G1603A
G1600-60013 G1600-60400 G1600-67311
*Interfacing the capillar requires an electrospray needle which is not included in this kit.
CE-ESI-MS Nebulizer Kit

Description CE-ESI-MS Nebulizer kit ES needle assembly CE-ESI sprayer Splitter assembly PEEK Ferrule, 360 m Nut, fingertight fitting and ferrule Flex loc element Gasket Ion kit (ammonium acetate) 2/pk 2/pk 2/pk Unit Part No. G1607A G1607-60041 G1607-60001 G1607-60000 5022-2141 0100-1543 1520-0401 G1607-20030 8500-4410
CE and CE/MS
Capillaries for CE/MS

Description CE-MS capillary, base-fused silica 50 m id, I=21.6 cm, L=125 cm CE-MS capillary, PVA-coated 50 m id, I=21.6 cm, L=125 cm CE-MS capillary, PVA-coated 75 m id, I=21.6 cm, L=125 cm Unit 2/pk 1/pk 1/pk Color Code Part No. Green Blue Blue G1600-67311 G1600-67219 G1600-67319
657

Accessories
Agilent CE System Start-up and Test Kits

Chemical test kits and validation packages are available to help comply with regulatory and quality standards. The Installation Qualification (IQ) Chemical Kit and Hardware Start-up Kit which are shipped with new instruments are useful for rapidly verifying system functionality. For rigorous testing, the Operational Qualification (OQ)/Performance Verification (PV) Kit can be used to verify DAD noise, drift, linearity, and wavelength accuracy, and replenishment functionality. The OQ/PV kit is only part of the validation services available from Agilent Technologies. When implemented by qualified Agilent personnel, our service packages can be used to help validate your Agilent CE system.
Agilent CE System Kits

Description Agilent CE Capillary Start-Up Kit: Includes one Agilent Extended Light Path capillary (L 64.5 cm, id 50 m), one standard capillary (l 56 cm, id 50 m), one test capillary (L 48.5, l 40 cm, id 50 m), one alignment interface for standard capillaries (id 50 m), and one alignment interface for Agilent Extended Light Path capillaries (id 50 m) Agilent CE Installation Qualification (IQ) Chemical Kit*: Includes buffer (20 mM borate, pH 9.2, 100 mL), test sample (4-(hydroxy)acetophenone 1 mM, 2 mL), capillary conditioning solution (0.1 N sodium hydroxide, 100 mL) Agilent CE Operational Qualification (OQ) Performance Verification (PV) Chemical Kit: Includes buffer (20 mM borate, pH 9.3, 100 mL), test samples (0.1, 0.5, 1.0, and 5.0 mM 4-(hydroxy)-acetophenone, 2 mL ea.), capillary conditioning solution (0.1 N sodium hydroxide, 100 mL), test capillary (L=48.5 cm, l=40 cm, id=50 m), diskette with methods, sequence, spectral library, and instruction manual Part No. G1600-68706
5063-6514
5063-6515
CE and CE/MS
OQ/PV Chemicals only Kit includes: buffer (20 mM borate, pH 9.2, 100 mL), test samples (0.1, 0.5, 1.0, and 5.0 mM 4-(hydroxy)-acetophenone, 2 mL ea.)
5063-6520
658

Accessories Window Etching Tool
The window etching tool is designed for fast, convenient and reproducible preparation of detection windows on fused silica capillaries. The polymide coating is removed without destroying the inner polymeric coating. It contains 3 glass blocks with fine grooves controlling precisely the size of the windows.
Window Etching Tool

Description Window etching tool Unit 3/pk Part No. 590-3003
Alignment Interfaces and Capillary Cassette

Agilent Technologies alignment interfaces are an integral part of the Agilent diode-array detection system. These interfaces contain optical slits which are precisely matched to the capillary inner diameter for optimized sensitivity and linear detection range. In combination with the capillary cassette, alignment interfaces simplify capillary exchange, protect the fragile detection window and ensure exact alignment of the window in the detector. Quick-change cassette allows capillary exchange in less than a minute. Note: The color code of the alignment interface must match with the color code of the capillary's built-in alignment stopper.
Alignment Interface
Alignment Interfaces
Description Color Code Corresp. Capillary Part No.
Alignment interface for standard capillaries with:
50 m id Green 75 m id, Blue 100 m id
Green Blue & Gray
G1600-60210 G1600-60310 G1600-60150 G1600-60230 G1600-60330 G1600-60400
Alignment interface for Agilent 25 m id Extended Light Path capillaries with: 50 m id 75 m id Nonmetallic alignment interface for CE/MS (360 od capillary)
Black Black Red Red Yellow Yellow Blue Blue & Gray
CE and CE/MS
Note: 75, 100 m id capillaries use the same interface (color blue). PVA coated 50 and 75 m id capillary for CE-MS use the same nonmetallic interface with color code blue for use with standard cap extended light path capillaries and the high sensitivity detector cell.
Cassette
Description Capillary cassette Part No. G1600-60002
Optical Filter
Description Optical filter: 260 nm diode array filter for DNA analysis with polyacrylamide-filled capillaries and oligonucleotide analysis Part No. G1600-62700
659
CE and CE/MS Capillaries

PAGE
PAGE Gel-filled Capillaries for High Resolution DNA Fragments Analysis

PAGE poly-acrylamide gel-filled capillaries are the most direct vehicles to transfer all of your applications from slab gel to CE, enjoying the automation, high speed, high resolution, and quantitative advantages of CE. The capillaries are ideal for high resolution separations of oligonucleotides, single-stranded and double-stranded DNA fragments, polymerase chain reaction (PCR) products, sequencing reaction products and oligosaccharides. PAGE capillaries are available in three different pore sizes. The size of the molecular sieving pores is controlled by the monomer concentration (%T) and the degree of polymer crosslinking (%C). Gels with higher %T and %C values have smaller pores and are, therefore, more effective at resolving smaller molecules. PAGE-10 (10%T, 0%C) capillaries provide high resolution capabilities for separation of antisense therapeutic agents, primers and probes as well as oligonucleotides. PAGE-5 (5%T, 5%C) allows single base resolution of oligonucleotides [pd(A)] ranging from 20-150 bases while PAGE-3 allows fast analysis of larger DNA fragments. Three different PAGE kits are available. For your convenience, PAGE capillaries and PAGE buffers can be purchased together or separately. To achieve the highest reproducibility and provide optimal capillary longevity, use PAGE buffer with PAGE capillaries.
PAGE Starter Kit

Includes 3 PAGE capillaries, 75 cm total length, 50 cm effective length PAGE pd(A)25-30, 40-60 oligonucleotide standard for PAGE-3 and Page-5 kits PAGE pd(A)25-30 oligonucleotide standard for PAGE-10 kit PAGE buffer, 2 x 237 mL
PAGE capillary in Kit PAGE-5 (10% T, 0% C) PAGE-5 (5% T, 5% C) PAGE-3 (3% T, 3% C) ID (m) 100 75 75 Part No. 192-1311 192-5211 192-3211
CE and CE/MS
PAGE Basic Kit

Includes 3 PAGE capillaries, 75 cm total length, 50 cm effective length PAGE pd(A)25-30, 40-60 oligonucleotide standard for PAGE-3 and Page-5 kits PAGE pd(A)25-30 oligonucleotide standard for PAGE-10 kit
PAGE capillary in Kit PAGE-10 (10% T, 0% C) PAGE-10 (5% T, 5% C) PAGE-3 (3% T, 3% C) ID (m) 100 75 75 Part No. 191-1311 191-5211 191-3211
660

PAGE Buffer Solutions and Oligo Standards
Description PAGE Tris-borate and urea buffer for PAGE-10, 4 x 237 mL PAGE Tris-borate and urea buffer for PAGE-3 and Page-5 kits, 4 x 237 mL PAGE pd(A)25-30, 40-60 oligonucleotide standard for PAGE-3 and Page-5 kits, 3 x 50 L Part No. 590-4005 590-4001 590-4000
Note: The PAGE capillaries are not pre-aligned for the P/N G1600A CE system. To cut them to the correct length use the P/N 5183-4669 CE column cutter.
Standard Bare Fused-Silica Capillaries for G1600A CE Instrument

Fused-silica capillaries are the heart of CE. Pre-aligned capillaries from Agilent Technologies are designed and optimized for ease of use and reliability. All capillary ends are cut to a smooth, mirror-like finish. In addition, the polyimide outer-coating is removed from the ends. These processes ensure minimal sample adsorption and help maintain sharp peak shapes. All capillaries have a pre-made detection window and a built-in alignment stopper which allows rapid and precise insertion in the alignment interface.
Standard Bare Fused-Silica Capillaries (2/pk)

Capillary ID (m) Total Lengths (cm) Effective Lengths (cm) Color Code Part No.
50
33 48.5 64.5 80.5 112.5 33 48.5 64.5 80.5 112.5 33 48.5 64.5 80.5 112.5
24.5 40 56 72 104 24.5 40 56 72 104 24.5 40 56 72 104
Green Green Green Green Green Blue Blue Blue Blue Blue Gray Gray Gray Gray Gray
G1600-63211 G1600-60211 G1600-61211 G1600-62211 G1600-64211 G1600-63311 G1600-60311 G1600-61311 G1600-62311 G1600-64311 G1600-63411 G1600-60411 G1600-61411 G1600-62411 G1600-64411
CE and CE/MS
75
100
Helpful Hint: Different inner diameters of capillaries need to use different alignment interfaces to guarantee optimal detection. The color coding of the capillary and the alignment interface allow you to easily combine the correct interface with the capillary.
661
CE and CE/MS Capillaries Extended Light Path Capillaries (Bubble Cell Capillaries) Bare Fused-Silica
Use Agilent Technologies extended light path capillaries (bubble cell capillaries) to improve sensitivity 3- to 5-fold over standard capillaries. With extended light path capillaries the inner diameter is increased only at the detection window, offering the sensitivity of a wide inner diameter capillary and the low current generation of a narrow one. Resolution is not sacrificed when used with Agilent Technologies' matching optical alignment interfaces.
Agilent Extended Light Path Capillaries Bare Fused-Silica (2/pk)

Capillary Total Effective Bubble Optical Path Color ID (m) Lengths (cm) Lengths (cm) Factor Length (m) Code Part No.
25
48.5 64.5 80.5 48.5 64.5 80.5 112.5 48.5 64.5 80.5 112.5
40 56 72 40 56 72 104 40 56 72 104
5 5 5 3 3 3 3 2.7 2.7 2.7 2.7
125 125 125 150 150 150 150 200 200 200 200
Black G1600-60132 Black G1600-61132 Black G1600-62132 Red Red Red Red Yellow Yellow Yellow Yellow G1600-60232 G1600-61232 G1600-62232 G1600-64232 G1600-60332 G1600-61332 G1600-62332 G1600-64332
50
75
Note: All capillaries come 2/pk
Universal Bare Fused-Silica

These are capillaries with a window and 75 cm effective length and 363 m od, fitting into any CE instrument. To cut them to the correct length we recommend using the P/N 5183-4669 CE column cutter.
CE and CE/MS
Universal Bare Fused-Silica Capillaries

Capillary ID (m) Total Length (cm) Effective Length (cm) Part No.
20 50 75 100
100 100 100 100
75 75 75 75
190-0431 190-0131 190-0231 190-0331
Bulk Fused-Silica Capillaries, 363 m od

Capillary ID (m) Total Length (m) Part No.
20 50 75
5 5 5
160-2660-5 160-2650-5 160-2644-5
662
CE and CE/MS Capillaries Poly(vinyl alcohol) (PVA) Coated Capillaries

PVA coated capillaries contain a permanently adsorbed layer of poly(vinyl alcohol). This coating minimizes hydrophobic and electrostatic solute/wall interactions and eliminates electroosmotic flow (EOF). Using a proprietary deposition process, the PVA coating is stable over a wide pH range even under basic conditions from 2.5 to 9.5. This stability allows use of a wide range of common CE buffers. Because the silica surface is covered, many proteins and amines can be analyzed without the peak tailing found with uncoated capillaries. In addition, since EOF is eliminated, cumbersome washing procedures are unnecessary and migration time reproducibility can be improved. Each batch of PVA coated capillaries is rigidly tested by Agilent Technologies and includes a representative electropherogram to assure quality. The color coding of the capillary (alignment stopper) and the alignment interfaces allows you to easily combine the correct interface with the capillary. Capillaries for non-Agilent CE system users have removable alignment stoppers without color code.
Agilent Capillary Electrophoresis System Users*

Capillary ID(m) Total Length (cm) Effective Length (cm) Bubble Factor Optical Path Length (m) Color Code Part No.
50
64.5 125
56 3 21.6 72 56 21.6 40 56
None 3 None None None None None None
50 Standard 150 Extended 50 75 1200 75 100 Standard 100 Standard
Green Red Blue
G1600-61219 G1600-61239 G1600-67219 for CE-MS G1600-62318 G1600-68319 for HSDC G1600-67319 for CE-MS G1600-60419 G1600-61419
*Not compatible with Borate buffers
75
97 64.5 125 48.5 64.5
Blue Gray Gray
100
Note: The PVA capillaries for CE-MS have a blue alignment stopper matching the blue color code of the alignment interface for MS-UV Det. The alignment stopper of the 50-id PVA capillary for CE-MS has a black dot for easy identification.
CE/MS
Non-Agilent Capillary Electrophoresis System Users*

Capillary ID (m) Total Lengths (cm) Effective Lengths (cm) Bubble Factor Optical Path Lengths (m) Part No.
50 100
71 56 71
60 45 60
None 3 None None
50 150 100 100
G160U-61219 G160U-61239 G160U-60419 G160U-61419

*Not compatible with Borate buffers
Note: When extended pathlength capillaries are used in non-Agilent systems, loss of resolution may be found if the axial slit width is not reduced. In Agilent systems the alignment interface contains properly matched slits to maintain resolution.
663
CE and CE/MS Capillaries CEP Coated Capillaries

CEP capillaries contain a permanently bonded polymer coating. This CEP coating shields the silanol functionality of the capillary surface and helps prevent sample adsorption. Additionally, EOF is nearly eliminated, making the capillary ideal for applications such as DNA separations with sieving polymer buffers. Elimination of EOF also simplifies analysis of anions and organic acids by direct UV detection. Without EOF-reduction, highly mobile ions such as nitrate can migrate in the opposite direction as the slower, longer chain acids. The CEP coated capillary is stable from pH 2 to 7-8. It can be used with borate buffers offering a different surface functionality to help alleviate sample adsorption. Each batch of CEP coated capillaries is rigidly tested by Agilent Technologies and each capillary includes a representative electropherogram to assure quality.
CEP Coated Capillaries (2/pk)

Capillary ID (m) Total Lengths (cm) Effective Bubble Lengths (cm) Factor Optical Path Lengths (cm) Part No.
75
97
72
None
75
G1600-62318
Cross-linked and Bonded SIL Capillaries

SIL-FC and SIL-DNA Capillaries With Windows
A series of coated capillaries specifically designed for CE which are prepared by cross-linking and bonding a novel, proprietary flourocarbon (FC) polymer. SIL-FC capillaries are chemically inert, hydrophobic, and stable from pH 2.5-10.0. These capillaries are a must-have for cIEF, protein, peptide and carbohydrate separations, as well as replaceable gel CE applications such as oligonucleotides, DNA fragments, and PCR product separations.
CE and CE/MS
SIL-DNA capillaries are also coated with an FC polymer but have a 75 m id to accommodate the viscosity of entangled polymer solutions. All SIL capillaries are batch tested to ensure the highest performance and reproducibility.
664

SIL-WAX Capillaries with Windows
SIL-WAX features a modified, polyethylene oxide, hydrophilic coating made through a special cross-linking and bonding process. The coating effectively masks active silanol sites, offering exceptional efficiency, resolution, peak shape and reproducibility. The highly stable coating and near-zero EOF of SIL-WAX makes the capillary ideal for CE-MS, and protein and peptide separations from pH 25.
Total Length (cm) Effective Length (cm) Film Thickness (m)
Capillary
Capillary ID (m)
Unit
Part No.
SIL-FC SIL-DNA SIL-WAX SIL-WAX
50 75 50 100
80 65 100 100
50 50 75 75
0.075 0.075 0.1 0.1
3/pk 2/pk 2/pk 2/pk
194-8111 199-2602 196-7203 197-7202
Bulk SIL-DB Capillaries

The SIL-DB coated capillaries are available as SIL-DB-1 and SIL-DB-17. In combination with a cellulose based buffer system, SIL-DB coated capillaries have been widely used in cIEF applications, PCR product and DNA fragment separation and many other CE applications which require reduced EOF.
Capillary Capillary ID (m) Length (m) Film Thickness (cm) Part No.
SIL-DB-1 SIL-DB-1 SIL-DB-1 SIL-DB-17 SIL-DB-17 SIL-DB-17
50 50 100 50 100 100
10 10 10 10 10 10
0.05 0.20 0.10 0.10 0.10 0.20
126-1012 126-1013 127-1012 126-1713 127-1712 127-1713
CE and CE/MS
Capillaries for Capillary Electrochromatography (CEC)

Capillary electrochromatography is a hybrid of CE and LC and can be performed in the Agilent CE system. Using CE capillaries packed with LC stationary phases, CEC offers the loadability and selectivity of LC and the high efficiency of CE. Using the high pressure capabilities of the Agilent CE system, both ends of the CEC capillary can be pressurized. This process prevents outgassing upon application of high voltage and significantly extends capillary lifetime.
665
CE and CE/MS Capillaries/Solutions Kits

Standard Packed Capillaries for CEC (2/pk)
Packing Material, Particle Size Capillary ID (m) Total Length (cm) Effective Length (cm) Color Code Detection Part No.
CEC-Hypersil C18, 3 m* CEC-Hypersil C8, 3 m CEC-Hypersil Phenyl, 3 m
100 100 100
33.5 48.5 33.5 48.5 33.5 48.5
25 40 25 40 25 40
Gray Gray Gray Gray Gray Gray
Standard Standard Standard Standard Standard Standard
5063-6512 5063-6513 5063-6535 5063-6540 5063-6536 5063-6541
*The color coding of the capillary (alignment stopper) and the interface allows you to easily combine the correct alignment interface with the capillary.
Packed CEC Capillaries for use with High Sensitivity Detection Cell (HSDC)*
Packing Material, Particle Size Capillary ID Effective Length (cm) Detection Part No.
CEC-Hypersil C18, 3 m CEC-Hypersil C8, 3 m
100 100
25 40 25 40
HSDC HSDC HSDC HSDC
5063-6537 5063-6542 5063-6538 5063-6543
*Capillary pack contains 2 capillary pairs with a packed inlet capillary and an empty 8.5 cm outlet capillary (100 um id) each. For test sample order: P/N 01080-68704
Helpful Hint: For more information, see our selection of kits and fittings for the high sensitivity detection cell (HSDC) (page 655 in this section).
Solutions Kits for CE

CE and CE/MS
Agilent Technologies CE Solutions Kits include all you need to begin your CE analysis: buffers, capillaries, conditioning solutions, test samples, and methods. Each kit is designed to take advantage of the automation of the Agilent CE system to make your time in the laboratory more efficient. All kits are prepared using the same quality procedures as our buffers and are thoroughly tested and supported. While the kits have been optimized for use with the Agilent CE system, they may be used with virtually any commercial or home-built CE system.
Inorganic Anion Solutions Kit

Component Inorganic Anion Solutions Kit Buffers and solutions: Inorganic anion buffer CE water 0.1 N NaOH 1.0 N NaOH Unit 1 250 mL 500 mL 250 mL 250 mL 2/pk 1 mL Part No. 5063-6511 8500-6797 5062-8578 5062-8575 5062-8576 G1600-62211 5062-8524
Capillary: Bare fused-silica capillary, id=50 m, I=72 cm Test mixture: Anion test mixture (Cl, Br, SO4, NO3, NO2, F, PO4), also used for HPLC
Note: The following part should be ordered separately for use with the Agilent CE System: Alignment interface for standard 50 m id extended light path capillary (P/N G1600-60210).
666
CE and CE/MS Solutions Kits

Organic Acids Solutions Kit
Component Organic Acids Solutions Kit Buffers and Solutions: Organic acids buffer CE water 1.0 N NaOH Unit 1 250 mL 500 mL 250 mL 2/pk 20 mL Part No. 5063-6510 8500-6785 5062-8578 5062-8576 G1600-62311 8500-6900
Capillary: Bare fused-silica capillary, id=75 m, I=72 cm Test Mixture: Organic acids test mixture (malate, succinate, lactate, 1000 ppm each)
Note: The following part should be ordered separately when used with the Agilent CE system: Alignment interface for 75 m id capillary (P/N G1600-60310).
Forensic Anions Kit

Component Forensic anion kit Agilent basic anion buffer Inorganic anion test mixture Includes: 1000 ppm each of fluoride, chloride, bromide, nitrite, nitrate, sulfate; and 2000 ppm phosphate Bare fused silica capillary: 50 m, 1-104 cm, L=112, 5 cm) CE water 5 x 50 mL 10 mL Unit Part No. 5064-8208 5064-8209 5062-8524
2 pk 1 x 500 mL
G1600-64211 5062-8578
Cation Solutions Kit

Component Cation Solutions Kit Cation Buffer for CE Water for CE Capillary* Bare fused silica capillary, extended light path BF3, 50 m id, I=56 cm, L=64.5 cm Text Mixture Cation Standard 250 mL 500 mL Unit Part No. 5064-8206 5064-8203
CE and CE/MS
5062-8578
2/pk 25 mL
G1600-61232 5064-8205
*Alignment interface P/N G1600-60230 must be ordered spearately (BF3)
667
CE and CE/MS Solutions Kits

Agilent Plating Bath Analysis Kit
Component Plating bath analysis kit Plating bath analysis buffer Plating bath test mixture Sulfate Malate Hypophosphite Phosphite Lactate Nickel Bare fused silica capillary (id=50 m, I=72 cm, L=80.5 cm) CE water 250 mL 20 mL 1000 ppm* 1000 ppm* 1000 ppm* 1000 ppm* 1000 ppm* 611 ppm* 2/pk 500 mL Unit Part No. 5064-8228 5064-8236 5064-8237
G1600-62211 5062-8578
*Made from NiSO4 6H2O
Note: The following part should be ordered separately for use with the Agilent CE System: Alignment interface for standard 50 m id extended light path capillary (P/N G1600-60210). Helpful Hint: CE applications can be found on the Agilent website at www.agilent.com/chem
CE and CE/MS
668
7500 ICP-MS Supplies

Gas Line Supplies
Optimizing Sensitivity and Performance

The Agilent 4500 ICP-MS system has been an industry standardthe best selling system in the world. Now the new 7500 family of instruments has advanced the state of the art, in sensitivity, performance, and ease of use. Agilent is a trusted partner of all its ICP-MS system users. All of our ICP-MS parts and supplies are designed, manufactured and tested to the most rigorous specificationsfor confidence in a perfect fit for uncompromised performance. As you can see in this section, Agilent is a single-source supplier. There is a listing here for every part and supply youll ever need for your Agilent ICP-MS system, including gas lines, peristaltic pump supplies, nebulizers, vacuum and interface supplies, plus single and multipleelement reference standardsall readily available and competitively priced.
Agilent 7500 ICP-MS Supplies

The 7500 Series is the ultimate in trace element measurement, combining high performance with unparalleled sample introduction flexibility to give a practical and reliable problemsolving tool. Building on the award-winning and best-selling 4500, the Agilent design team has taken the best of the previous model and added new innovative features. From its durable stainless steel chassis, through the novel digital synthesized RF scanning quadrupole to the high speed, wide dynamic range detection system, the 7500 Series is an exceptionally powerful highperformance instrument with the robustness and reliability required of a routine workhorse. Note: Most 7500 parts listed in this section are common to the 7500 a/i/s/c platforms. Parts unique to the 7500c are listed in separate tables.
7500 ICP-MS
Gas Line Supplies

Description Specs Usage Unit Part No.
Inner sleeve for carrier/ auxiliary/blend gas Inner sleeve for plasma gas line Argon tubing assembly, 5 m Teflon tubing, one in-line filter and connector set Plasma and auxiliary gas line tubing, Tygon 3.17 mm id, 6.35 mm od, 35 cm (for plasma gas line). 2.38 mm id, 3.96 mm od, 35 cm (for auxiliary gas line), 1 ea. Connects the plasma gas and auxiliary gas lines to the torch.
5/pk 5/pk
5042-0922 5042-0923 G1820-65023
ICP-MS
G1833-65428
Connector for end cap and gas line Tubing for carrier/ makeup gas line, PTFE 3 mm id, 4 mm od, 350 mm 2/pk
5063-5263 G1833-65414
669

Connectors/Peristaltic Pump Tubing and Supplies
Connectors
Description Specs Usage Part No.
Connectors, Teflon (2/pk) Carrier gas connector for Babington Nebulizer Blend gas line connector, polypropylene Carrier gas line connector, polypropylene
6 mm and 4 mm L-shaped Teflon connector with a ferrule and O-ring
For plasma and auxiliary gases For connection with 4 mm diameter tubing
G1820-65027 G1820-65214
Connectors for Plasma and Auxiliary Gases P/N G1820-65027
Connects 4 mm tubing G1820-65119 with Babington and Cross Flow Nebulizers Includes ferrules and Viton O-ring Connects 4 mm carrier gas tubing to Concentric Nebulizer G1820-65052
Peristaltic Pump Tubing and Supplies

Description Specs Usage/Replacement Unit Part No.
Tygon tubing
1.02 mm id, length = 400 mm 1.52 mm id, length = 400 mm 0.19 mm id, length = 400 mm
Standard tubing for sample introduction Standard tubing for drainage; replacement required when tubing becomes stretched
12/pk
G1820-65215
Gas Line Connector P/N G1820-65052
Ismaprene tubing ISTD tubing, Tygon On-line ISTD addition Kit Drain tubing kit Drain tank
12/pk
G1820-65216
Used for on-line internal standard 12/pk addition. Replacement is required when tubing is stretched Permits mixing of sample solution with internal standard solution at a fixed rate using peristaltic pump Used to drain spray chamber to waste
G1820-65220
G1820-65479
G1820-65221 G1820-65016 G1820-65505
Carrier Gas Connector P/N G1820-65214
Drain tank for organic solvent Tubing for drainage 4 mm id, 2.5 m
G1820-65515
ICP-MS
Helpful Hints: Use Agilents drain tubing kit to replace a heavily contaminated drain tubing assembly or when pumping efficiency is low. Leaving the tubing attached to the pump will shorten the lifetime of the tubing. It is recommended to release the peristaltic pump tubing when the system is not in use.
Blend Gas Line Connector P/N G1820-65119
670

Integrated AutoSampler Supplies/ISIS Supplies
Integrated AutoSampler Supplies
Description Usage Unit Part No.
Type A Tray 89 Position Type B Tray 53 Position Type C Tray 18 Position 6 mL vials 18 mL vials 50 mL vials PFA needle Rinsing Bottle, 100 mL Type D Tray Type E Tray Polyethylene vial, 2 mL PFA vial, 1.5 mL
For 6 mL vial For 18 mL vial For 50 mL vial For Type A Tray 89 Position For Type B Tray 53 Position For Type C Tray 18 Position 200/pk 55/pk 20/pk 6/pk For 6 mL and 18 mL vials For Type D Tray For Type D Tray 200/pk 10/pk
G3160-65300 G3160-65301 G3160-65302 G3160-65303 G3160-65304 G3160-65305 G3160-65306 G3160-65307 G3160-65308 G3160-65309 G3160-65315 G3160-65317
G3138A/G3148A Integrated Sample Introduction System (ISIS) Supplies

Peristaltic pump tubing, Tygon Peristaltic pump tubing, Tygon Sample tubing ETFE Tee joint Cross joint Union joint Sample tubing, PTFE Teflon nut Front and back ferrule Teflon nut Front and back ferrule
0.89 mm id, 3 stops 0.25 mm id, 2 stops 0.8 mm id, 1.6 mm od
For Agilent 7500 Pump For ISIS Pump
12/pk 12/pk
5064-8014 5064-8015 5064-8016 5064-8017 5064-8018 5064-8019
2 mm id, 3 mm od For 3 mm od tubing For 3 mm od tubing For 1.6 mm od tubing For 1.6 mm od tubing
5064-8020 5064-8021
ICP-MS
5064-8022 5064-8023 5064-8024
671

ISIS Supplies
G3138A/G3148A Integrated Sample Introduction System (ISIS) Supplies (continued)
Peristaltic pump tubing adapter Tubing clamps Peristaltic pump tubing, Tygon Spiral tubing Wrench Tag Peristaltic pump tubing, Tygon Standard accessory parts Standard accessory parts Standard accessory parts Joint holder Joint holder Sample line connector (Cross joint and earth block) Rotor seal Nut Ferrule Mixer For valve For valve For valve 0.25 mm id, 3 stops For valve maintenance For tubing identification For Agilent 7500 Pump For high sample throughput For auto dilution For discrete sampling For Agilent 7500 pump For ISIS pump 10/pk 12/pk 1.02 mm id, 2 stops For ISIS Pump Small, 10/pk Large, 5/pk 12/pk
5064-8026 5064-8027 5064-8028 5064-8029 5064-8032 5064-8033 5064-8034 G3138-65000 G3138-65001 G3138-65002 G3138-65102 G3138-65103 G3138-65104
G3138-65117 G3138-65118 G3138-65119 G3138-65121 G3138-65122 G3138-65126
ICP-MS
Sample loop 100 L Tubing adapter Sample tubing, PFA Plastic tray 0.3 mm id, 1.6 mm od
5064-8013 G3138-65125
672

Nebulizers and Nebulizer Supplies

The nebulizer disperses the sample solution from the peristaltic pump as a fine mist into the spray chamber using a stream of argon gas. There are three types of nebulizers available for the 7500: The Babington Nebulizer, Concentric Nebulizer, and Cross Flow Nebulizer.

Babington Nebulizer Includes O-rings (P/N G1820-65199) and sample tubing (P/N G1820-65276) Sample tubing with connector Tool included
Use to introduce samples with high matrix and high total salts. Clean when contaminated or when orifices are clogged. Use with Babington Nebulizer. Replace the tubing when it is heavily contaminated by high matrix samples Replace when memory effects do not disappear after cleaning. Insert nebulizer in middle of end cap all the way through. Attach end cap to spray chamber with TOP mark facing up. Use with clean samples only. Not resistant to hydrochloric acid. Clean with a 5% HNO3 (v/v) bath; do not use ultrasonic bath. Recommended for trace analysis of clean samples. Lower contamination level than Pyrex. Clean using 5% HNO3 (v/v) bath; do not use ultrasonic bath. Connects the Concentric Nebulizer (Pyrex or Quartz) with the spray chamber. Replace when the capillary is broken or the nebulizer is damaged. Connects Concentric Nebulizer with sample line. Port with large hole connects to nebulizer; smaller port connects with sample tubing. Standard nebulizer for the inert kit. Resistant to hydrofluoric acid. Set carrier gas pressure to 500 kPa (5 kg/cm2 ). Clean with 5% HNO3 (v/v) bath. Replace when memory effects remain after cleaning. Use for the end cap of the Babington Nebulizer, Cross Flow Nebulizer and Concentric Nebulizer Sample tubing for Cross Flow Nebulizer. Replace when the tubing is bent, damaged, or when there are serious memory effects in the sample introduction area. 5/pk
G1820-60453
G1820-65276
End cap for Includes O-ring and blend gas Babington Nebulizer line connector
G1833-65476
Pyrex Concentric Nebulizer
Use with P/N G1820-65052 Argon gas connector, P/N G1820-60160 Connector kit, P/N G1820-65121 end cap Use with P/N G1820-65337 Quartz spray chamber, P/N G1820-80237 Quartz connecting pipe Includes blend gas line connectors, O-rings for end cap, O-rings for fittings to end cap, PTFE Includes O-rings
G1820-65030
Quartz Concentric Nebulizer End cap for Concentric Nebulizer Connector kit
G1820-65138
G1833-65475
G1820-60160
Cross Flow Nebulizer
Includes bushing, carrier gas line connector, blend gas line connector, sample tubing, and gas line tubings with inner sleeves. O-rings
G1833-65462
O-rings for end cap, Viton Sample tubing
G1820-65198
ICP-MS
G1820-65141
Nebulizer sample tubing, Teflon
0.5 mm id, 1.56 mm od, 5 m
Used for standard sample introduction. Replace when the tubing is bent, damaged, or when there are serious memory effects in the sample introduction area.
G1820-65105
Babington Nebulizer removal tool Sample tubing for on-line ISTD addition, PFA 0.3 mm id, 1.6 mm od, 3 m
G1820-65345 G1820-65478
673

Nebulizers and Nebulizer Supplies/Spray Chambers
Nebulizers and Nebulizer Supplies (continued)
O-ring kit for Concentric Nebulizer O-rings for organic solvents O-rings for organic solvents Ferrule and Viton O-ring kit for carrier gas line connector
Viton O-rings, 2 types For end cap For use with the Concentric Nebulizer For use with the Babington and Concentric Nebulizer
10 ea. 2/pk
G1820-65491 G1820-65518 G1820-65520 G1820-65533
Babington Nebulizer P/N G1820-60453
Polypropylene Spray Chamber P/N G1833-65463
Spray Chambers
Quartz spray chamber for Babington Nebulizer Polypropylene spray chamber Quartz ball joint connector Clamps Bushing at spray chamber drain, Viton Connector for spray chamber drainage, Polypropylene Bushing at spray chamber drain T-connector for optional gas introduction, Quartz Ball joint connector between torch and spray chamber, Polypropylene Sapphire Tube
Used for Babington, Concentric, and Micro-Concentric Nebulizers. Replace when memory effects remain after cleaning. Same usage as Quartz and is resistant to hydrofluoric acid. Replace when memory effects remain after cleaning.
G1820-65337 G1833-65463 G1833-65426 2/pk 5/pk G1833-65430 G1820-65482 G1820-65503
Requires 2 clamps Connects the Quartz spray chamber to the torch. (P/N G1833-65430) Used to hold connectors
ICP-MS
For use with organic solvents Between spray chamber and torch
G1820-65504 G1833-65427 G1833-65464
674

Torch and Components
Quartz torch: Comprised of three 2.5 mm id Concentric Quartz tubes through 1.5 mm id which streams of argon gas pass 1.0 mm id Three streams = plasma, auxiliary, and nebulizer (carrier) gas. Bonnet for ShieldTorch
Not suitable for samples containing hydrofluoric acid. Must be completely dried before installing and igniting the plasma. Replace if chipped, cracked, or distorted. Use with shield plate (P/N G1833-65406 or P/N G1833-65419) for ShieldTorch system. Replace if bonnet is cracked or damaged. System is used to drastically reduce argon gas-related polyatomic ion generation. Enables ultra trace analysis of elements sensitive to ion interference. 2/pk
G1833-65423 G1833-65424 G1833-65425
G1833-65421
Shield plate for ShieldTorch system, copper
G1833-65406
Long life shield plate for ShieldTorch Inert torch
Uses special material for longer lifetime than P/N G1833-65406
Use with the bonnet
G1833-65419
Inner tubing not included Quartz torch for inert sample introduction. Resistant to hydrofluoric acid. Torch must be completely dried before installing and igniting plasma. 2 mm id 2.5 mm id 1.5 mm id For inert torch For inert torch For inert torch Cooling water flows through coil to reduce torch temperature
G1833-65422
Platinum injector Platinum injector Platinum injector Work coil
G1833-65409 G1833-65415 G1833-65416 G1833-65432
ICP-MS
Quartz Torch P/N G1833-65423
Inert Torch P/N G1833-65422
675

Sampling and Skimmer Cones and Supplies
Nickel sampling cone O-ring for sampling cone Platinum sampling cone Platinum sampling cone, 18 mm Nickel skimmer cone for 7500 a/i/s Platinum skimmer cone for 7500 a/i/s Skimmer base (with screws) for 7500 a/i/s Screws for skimmer base Interface wrench Sampling cone wrench Nickel sampling cone for T-mode Nickel skimmer cone for T-mode
Copper base Viton O-ring Copper base 18 mm with copper base The point of the skimmer cone is easily deformed. A cone deformed cone will greatly affect performance. If the O-ring becomes stuck, wipe it off with methanol. Copper base Stainless steel Brass Must be mounted on brass skimmer base. Stainless steel holds the standard Nickel skimmer cone. Do not use with Platinum cone. Brass holds the Platinum or Nickel skimmer cone. Used to install the sampling base to the vacuum chamber Round type Used to remove and install the skimmer cone Used to remove and install sampling cone 4/pk Seals the sampling cone to the vacuum chamber.
G1820-65238 G1820-65025 G1820-65239 G1820-65360 G1820-65050
G1820-65237 G1833-65407 G1833-65408 G1820-65435 G1833-65079 G1833-65405 G1820-65480 G1820-65481
7500c Sampling and Skimmer Cones and Supplies

Description Usage/Replacement Part No.
Skimmer cone, Platinum Skimmer cone, Nickel (with screws) Skimmer base, SS (with screws) Skimmer base, Brass (with screws)
Platinum cone and Nickel base. Replace when tip of cone is damaged
G1833-65092 G1833-65497 G1833-65498 G1833-65505
ICP-MS
Note: Most 7500 parts listed in this section are common to the 7500 a/i/s/c platforms. Parts unique to the 7500c are listed in separate tables.
Sampling and Skimmer Cones
676

Ion Lens System Components

7500a/i/s Ion Lens System Components
Description Specs Usage/Replacement Part No.
Extraction lens
Lens 1 Lens 2
Placed just behind the skimmer cone and mounted on the skimmer base. Extracts positive ions and accelerates them to the Einzel lens. Spare screws and spacers for the extraction lense
G1833-65417 G1833-65413
Screw/spacer set for extraction lens Omega lens Block of 5 lenses: omega bias, omega (+), omega (-), QP focus, and plate bias
G1833-65024
Separates photons and ions and steers ions onto the quadrupole MS Spare screws and spacers for omega lens
G1833-65418
Screw/spacer set for ion lens Polishing paper kit Waterproofed sheets, #400 and #1200, 5 of each
G1833-65025 G1833-65404
Used to clean the ion lenses and the interface cones
Extraction Lens 1 P/N G1833-65417
Extraction Lens 2 P/N G1833-65413
7500c Ion Lens System Components

ICP-MS
Screw kit for ion lens Octopole Lens cleaning kit Extraction lens Einzel lens 1 Einzel lens 2 Einzel lens 3 Octopole assembly Tubing for reaction gas 2/pk
G1833-65088 G1833-65094 G1833-65096 G1833-65499 G1833-65500 G1833-65501 G1833-65502 G1833-65503 G1833-65089
677
7500 ICP-MS
Electron Multiplier/Rotary Pumps and Supplies/Miscellaneous Components
Electron Multiplier
Description Specs Part No.
Electron multiplier
Converts incident ions into an amplified electric current to measure the ion signal. As voltage increases, amplification factor (gain) increases.
G1833-65420
Electron Multiplier P/N G1833-65420
Vacuum System: Rotary Pumps

The oil level on the pump should be checked every three months, when the plasma is ON. Maintain the oil level between the Max and Min levels on the gauge. Standard frequency for changing the oil is approximately every 4,500 hours. Warning: Be careful when changing oil because the oil may have absorbed dangerous substances. The oil in the rotary pump on the interface side of a 7500 equipped with a hydrofluoric acid resisting introduction system is especially dangerous. DO NOT TOUCH THE OIL WITH BARE HANDS.
Rotary Pumps and Supplies

Description Specs Unit Part No.
Oil, rotary pump Oil, rotary pump Oil mist filter kit Oil mist filter element O-ring for vacuum chamber, Viton Odor element for oil mist filter O-rings for vent valve, Viton
4L 1L For rotary pump, E2M18 For rotary pump, E2M18
6040-0798 6040-0834 3162-1056 1535-4970 5042-0901
For E2M18
5/pk 2/pk
5063-9153 G1833-65332
Miscellaneous Components
ICP-MS
Description
Specs
Part No.
Filter for cooling water Plastic tray for the sample introduction area Cooling water hose
One filter
G1820-65018 G1833-65412
10 m
G1833-65429
678

Gas Line Supplies/Connectors
Agilent 4500 ICP-MS Supplies

The Agilent Technologies 4500 ICP-MS System routinely achieves parts per trillion (ppt) level sensitivity. So you wont want to risk compromising that performance with anything less than ultra-high quality Agilent parts and supplies. Rigorously designed, manufactured, and tested to Agilents demanding specifications, these consumables are the perfect fit for the 4500. We offer every supply you need for the Agilent 4500 ICP-MS including gas lines, pumps, nebulizers, vacuum and interface supplies, and single and multiple-element reference standards. Its the guaranteed combination for optimizing your 4500 ICP-MS performance. To always maintain ppt level sensitivity, be sure to periodically replace your consumables according to recommended maintenance schedules.
ICP-MS 4500
Gas Line Supplies

Inner sleeve for carrier/auxiliary/blend gas Inner sleeve for plasma gas line Tubing for carrier blend gas, Teflon Tubing for plasma and auxiliary gases, Teflon Tubing for blend gas, Teflon Argon tubing assembly, includes Teflon tubing, one in-line filter, connector set Connector for end cap and gas line 3 mm id, 4 mm od, length 38 cm 3.17 mm id, 6.35 mm od, 2.38 mm id, 3.96 mm od, length 40 cm 1m 5m
5/pk 5/pk 4/pk 2/pk
5042-0922 5042-0923 G1820-65004 G1820-65003 G1820-65008 G1820-65023 5063-5263
Connectors
Description Specs Usage Part No.
Connectors, Teflon Gas line connectors for Cross Flow Nebulizer Carrier gas connector for Babington Nebulizer Blend gas line connector, Polypropylene Carrier gas line connector, Polypropylene
6 mm and 4 mm One Teflon and one polypropylene L-shaped Teflon connector with a ferrule and O-ring
For plasma and auxiliary gases Connects carrier and blend gas to the Cross Flow Nebulizer (P/N G1820-65033) For connection with 4 mm diameter tubing Connects 4 mm tubing with Babington and Cross Flow Nebulizers Connects 4 mm carrier gas tubing to Concentric Nebulizer
G1820-65027 G1820-65144 G1820-65214 G1820-65119
ICP-MS
G1820-65052
Connectors for Plasma and Auxiliary Gases P/N G1820-65027
Carrier Gas Connector P/N G1820-65214
Gas Line Connector P/N G1820-65052
Blend Gas Line Connector P/N G1820-65119
679

Peristaltic Pump Tubing and Supplies/Integrated Autosampler Supplies
Peristaltic Pump Tubing and Supplies
Tygon tubing Ismaprene tubing ISTD tubing, Tygon On-line ISTD Addition Kit Drain tubing kit Drain tank Drain tank for organic solvent Tubing for drainage
1.02 mm id, length = 400 mm 1.52 mm id, length = 400 mm 0.19 mm id, length = 400 mm
Standard tubing for sample introduction Standard tubing for drainage; replacement required when tubing becomes stretched Used for on-line internal standard addition. Replacement is required when tubing is stretched Permits mixing of sample solution with internal standard solution at a fixed rate using peristaltic pump Used to drain spray chamber to waste
12/pk 12/pk 12/pk
G1820-65215 G1820-65216 G1820-65220 G1820-65479 G1820-65221 G1820-65016 G1820-65505
4 mm id, 2.5 m
G1820-65515
Helpful Hints: Use Agilents drain tubing kit to replace a heavily contaminated drain tubing assembly or when pumping efficiency is low. Leaving the tubing attached to the pump will shorten the lifetime of the tubing. It is recommended to release the peristaltic pump tubing when the system is not in use.
Integrated AutoSampler Supplies

Description Usage Unit Part No.
Type A Tray 89 Position Type B Tray 53 Position Type C Tray 18 Position 6 mL vials 18 mL vials 50 mL vials PFA needle Rinsing Bottle, 100 mL
For 6 mL vial For 18 mL vial For 50 mL vial For Type A Tray 89 Position For Type B Tray 53 Position For Type C Tray 18 Position 200/pk 55/pk 20/pk 6/pk For 6 mL and 18 mL vials For Type D Tray For Type D Tray 200/pk 10/pk
G3160-65300 G3160-65301 G3160-65302 G3160-65303 G3160-65304 G3160-65305 G3160-65306 G3160-65307 G3160-65308 G3160-65309 G3160-65315 G3160-65317
ICP-MS
Type D Tray Type E Tray Polyethylene vial, 2 mL PFA vial, 1.5 mL
680

ISIS Supplies
G3138A/G3148A Integrated Sample Introduction System (ISIS) Supplies
Peristaltic pump tubing, Tygon Peristaltic pump tubing, Tygon Sample tubing ETFE Tee joint Cross joint Union joint Sample tubing, PTFE Teflon nut Front and back ferrule Teflon nut Front and back ferrule Peristaltic pump tubing adapter Tubing clamps Peristaltic pump tubing, Tygon Spiral tubing Wrench Tag Joint holder Joint holder Sample line connector (Cross joint and earth block) Rotor seal Nut Ferrule Mixer Sample loop 100 L Tubing adapter Sample tubing, PFA Plastic tray Peristaltic pump tubing, Tygon Standard accessory parts Standard accessory parts Standard accessory parts
0.89 mm id, 3 stops 0.25 mm id, 2 stops 0.8 mm id, 1.6 mm od
For Agilent 4500 pump For ISIS pump
12/pk 12/pk
5064-8014 5064-8015 5064-8016 5064-8017 5064-8018 5064-8019
2.0 mm id, 3.0 mm od For 3 mm od tubing For 3 mm od tubing For 1.6 mm od tubing For 1.6 mm od tubing Small 10/pk, Large 5/pk 1.02 mm id, 2 stops For ISIS pump For valve maintenance For tubing identification For Agilent 4500 pump For ISIS pump 10/pk 12/pk
5064-8020 5064-8021 5064-8022 5064-8023 5064-8024 5064-8026 5064-8027 5064-8028 5064-8029 5064-8032 5064-8033 G3138-65102 G3138-65103 G3138-65104 For valve For valve For valve G3138-65117 G3138-65118 G3138-65119 G3138-65121 G3138-65122 G3138-65126
0.3 mm id, 1.6 mm od 0.25 mm id, 3 stops For Agilent 4500 pump For High sample throughput For auto dilution For discrete sampling 12/pk
5064-8013 G3138-65125 5064-8034 G3138-65000 G3138-65001 G3138-65002
ICP-MS
681

Babington Nebulizer
Includes O-rings (P/N G1820-65199) and sample tubing (P/N G1820-65276)
Use to introduce samples with high matrix and high total salts. Clean when contaminated or when orifices are clogged. Use with Babington Nebulizer. Replace the tubing when it is heavily contaminated by high matrix samples Replace when memory effects do not disappear after cleaning. Insert nebulizer in middle of end cap all the way through. Attach end cap to spray chamber with TOP mark facing up.
G1820-60453
Sample tubing Tool included with connector End cap for Babington Nebulizer Babington Nebulizer kit Includes O-ring and blend gas line connector
G1820-65276 G1833-65476
Includes: Babington Nebulizer with O-rings (P/N G1820-65119, P/N G1830-65198) and sample tubing (P/N G1820-65276); end cap for carrier gas line connector (P/N 50635262); blend gas line connector removal tool (P/N G1820-65345) Use with (P/N G1820-65052) Argon gas connector, (P/N G1820-60160) Connector kit, (P/N G1820-6512) end cap Use with (P/N G1820-65337) Quartz spray chamber, (P/N G1820-80237) Quartz connecting pipe End cap for Concentric Nebulizer with blend gas line connectors, O-rings for end cap, O-rings for fittings to end cap, PTFE Use with clean samples only. Not resistant to hydrochloric acid. Clean with a 5% HNO3 (v/v) bath; do not use ultrasonic bath. Recommended for trace analysis of clean samples. Lower contamination level than Pyrex. Clean using 5% HNO3 (v/v) bath; do not use ultrasonic bath. Connects the Concentric Nebulizer (Pyrex or Quartz) with the spray chamber. Replace when the capillary is broken or the nebulizer is damaged. Used to create seal between Babington Nebulizer and end cap. 4/pk
G1820-65356
Pyrex Concentric Nebulizer Quartz Concentric Nebulizer End cap for Concentric Nebulizer O-Rings for Babington Nebulizer, Viton
G1820-65030
G1820-65138
G1820-65121
G1820-65199
Connector kit Includes O-rings
Connects Concentric Nebulizer with sample line. Port with large hole connects to nebulizer; smaller port connects with sample tubing. 2 ea.
G1820-60160
O-ring kit
ICP-MS
Viton O-rings, 2 sizes Smaller O-rings seal between the Concentric Nebulizer and center hole of the end cap; larger O-rings seal between the end cap and spray chamber. Replacement required when seal no longer effective. Includes bushing, carrier gas line connector, blend gas line connector, sample tubing, and gas line tubings with inner sleeves. Standard nebulizer for the inert kit. Resistant to hydrofluoric acid. Set carrier gas pressure to 500 kPa (5 kg/cm2 ). Clean with 5% HNO3 (v/v) bath. Replace when memory effects remain after cleaning. Use for the end cap of the Babington Nebulizer, Cross Flow Nebulizer and Concentric Nebulizer For Cross Flow Nebulizer. Replacement is required when the sample tubing is bent, damaged, or when there are serious memory effects in the sample introduction area.
G1820-65075
Cross Flow Nebulizer
G1833-65462
O-rings for O-rings end cap, Viton Sample tubing
5/pk
G1820-65198 G1820-65141
682

Nebulizers and Nebulizer Supplies/Spray Chambers
Nebulizers and Nebulizer Supplies (continued)
Nebulizer sample tubing, Teflon
0.5 mm id, 1.56 mm od, 5 m
Used for standard sample introduction. Replacement is required when the sample tubing is bent, damaged, or when there are serious memory effects in the sample introduction area.
G1820-65105
Babington Nebulizer removal tool Sample tubing for on-line 0.3 mm id, 1.6 mm od, 3 m ISTD Addition, PFA O-ring kit for Concentric Nebulizer O-rings for organic solvents O-rings for organic solvents Ferrule and Viton O-ring kit for carrier gas line connector Viton O-rings, 2 sizes For end cap For Concentric Nebulizer For Babington and Concentric Nebulizer 10 ea. 2/pk
G1820-65345 G1820-65478 G1820-65491 G1820-65518 G1820-65520 G1820-65533
Babington Type Nebulizer P/N G1820-60453
Polypropylene Spray Chamber P/N G1833-65463
Spray Chambers
Quartz spray chamber for Babington Nebulizer Pyrex spray chamber for Babington Nebulizer Polypropylene spray chamber Quartz spray chamber for Cross Flow Nebulizer Pyrex spray chamber for Cross Flow Nebulizer
Standard for the 4500
Used for Babington, Concentric, and MicroConcentric Nebulizers. Replace when memory effects remain after cleaning. Same usage as quartz but not recommended for trace analysis due to more impurities in Pyrex material. Replace when memory effects remain after cleaning. Same usage as quartz and is resistant to hydrofluoric acid. Replace when memory effects remain after cleaning. Used with Cross Flow Nebulizer. Replace when memory effects remain after cleaning. Same as quartz but due to impurities in Pyrex, not recommended for ultra-trace analysis. Replace when memory effects remain after cleaning. Standard spray chamber for inert kit with Cross Flow Nebulizer. Low boron background. Replace when memory effects remain after cleaning.
G1820-65337
G1820-65336
ICP-MS
G1820-65343 G1820-65339 G1820-65338
Polypropylene spray chamber for Cross Flow Nebulizer
G1820-65342
683

Spray Chambers/Torch and Components
Spray Chambers (continued)
Quartz ball joint connector Pyrex ball joint connector Clamps Inert connector kit O-rings, and clamp Tygon tubing Bushing at spray chamber drain (Viton) Connector for spray chamber Drainage, Polypropylene Bushing at spray chamber drain T-connector for additional gas, Quartz
Requires 2 clamps (P/N G1820-65007) Requires 2 clamps (P/N G1820-65007) Includes Tygon tubing, 7.9 mm id, 11.1 mm od, 23 mm
Connects the Quartz spray chamber to the torch. Connects the Pyrex spray chamber to the torch. Used to hold connectors. Connects polypropylene spray chamber and inert torch. Connects the polypropylene spray chamber with the inert connector kit. 10/pk 5/pk 2/pk
G1820-80237 G1820-80235 G1820-65007 G1820-65163 G1820-65164 G1820-65482 G1820-65503
For use with organic solvents Between spray chamber and torch
G1820-65504 G1820-80512

Quartz torch
Comprised of three Concentric Quartz tubes through which streams of argon gas pass. Three streams = plasma, auxiliary, and nebulizer (carrier) gas.
Standard torch with 4500; not suitable for samples containing hydrofluoric acid. Must be completely dried before installing and igniting the plasma. Replace if chipped, cracked, or distorted. Use with shield plate (P/N G1820-65185 or P/N G1820-65357) for ShieldTorch system. Replace if bonnet is cracked or damaged. System is used to drastically reduce argon gas2/pk related polyatomic ion generation. Enables ultra trace analysis of elements sensitive to ion interference. Use with the bonnet.
G1820-65028
Bonnet for ShieldTorch Shield plate for ShieldTorch Long life shield Uses special material for longer lifetime plate for than P/N G1820-65185. ShieldTorch
G1820-65026
G1820-65185
G1820-65357
ICP-MS
Inert torch
Inner tubing not included.
Quartz torch for inert sample introduction. Use with inner tubing P/N G1820-65013 or P/N G182065361 and adapter P/N G1820-65015. Resistant to hydrofluoric acid. Torch must be completely dried before installing and igniting plasma.
G1820-65035
Inert sample Includes one each of the following: Cross introduction kit Flow Nebulizer, polypropylene spray chamber assembly, torch and Platinum tube, adapter, and ten 23 mm Tygon tubes. Adapter for inert torch, PTFE Used with inert torch (P/N G1820-65035) and inner tubing (P/N G1820-65013 or 65361) for inert torch assembly.
G1820-65201
G1820-65015
684

Torch and Components/Sampling and Skimmer Cones and Supplies
Torch and Components (continued)
Platinum inner tubing for inert torch Sapphire inner tubing Work coil RF return strip Bonnet (for ShieldTorch w/alignment projection) Attachment tool for bonnet (P/N G1820-65497) Platinum inner tubing for inert torch Platinum inner tubing for inert torch Torch, Quartz Torch, Taper type
Resistant to hydrofluoric acid. Resistant to hydrofluoric acid.
Use with inert torch (P/N G1820-65035) and adapter (P/N G1820-65015) for inert torch assembly Use with inert torch (P/N G1820-65035) and adapter (P/N G1820-65015) for inert torch assembly. Cooling water flows through coil to reduce torch temperature. The RF return strip is located between the interface and torch box.
G1820-65013 G1820-65361 G1820-65061 G1820-65060 G1820-65497 G1820-65498
1.5 mm id 2.5 mm id 1.0 mm id 1.5 mm id
G1820-65501 G1820-65502 G1820-65528 G1820-65529
Quartz Torch P/N G1820-65028
Work Coil P/N G1820-65061

ICP-MS
Nickel sampling cone O-ring for sampling cone Platinum sampling cone Platinum sampling cone, 18 mm Nickel skimmer cone
Copper base Viton O-ring Copper base 18 mm with copper base
Standard on the 4500 Seals the sampling cone to the vacuum chamber
G1820-65238 G1820-65025 G1820-65239 G1820-65360
The point of the skimmer cone is easily deformed. A deformed cone will greatly affect performance. If the O-ring becomes stuck, wipe it off with methanol.
G1820-65265
685

Sampling and Skimmer Cones and Supplies/Ion Lens System Components
Sampling and Skimmer Cones and Supplies (continued)
Platinum skimmer cone Skimmer base Screws for skimmer base Interface wrench Interface wrench (Round type) Nickel sampling cone for t-mode Nickel skimmer cone for t-mode
Copper base Stainless steel Brass
Must be mounted on brass skimmer base. SS holds the standard Nickel skimmer cone. Do not use with Platinum cone. Brass holds the Platinum or Nickel skimmer cone. Used to install the skimmer base to the vacuum chamber Used to remove and install the sampling and skimmer cone Used to remove and install skimmer cone 4/pk
G1820-65237 G1820-60376 G1820-60104 G1820-65435 G1820-60393 G1883-65079 G1820-65480 G1820-65481
Nickel Skimmer Cone P/N G1820-65265

Extraction lens Einzel lens
Lens 1 Lens 2 Three lens plates
Placed just behind skimmer cone and mounted on skimmer base; Extracts positive ions and accelerates them to the Einzel lens The same voltage is applied to the first and third lens plates to get the same potential at entrance and exit (-100V). Changing the voltage of the mid lens affects the focal point of the ions. Separates photons and ions and steers ions onto the quadrupole MS
G1820-65110 G1820-65358 G1820-65062
Omega lens
Block of 5 lenses: omega bias, omega (+), omega (-), QP focus, and plate bias.
G1820-60399
Screw/Spacer set for ion lens
Spare screws and spacers for Einzel and omega lenses Waterproofed sheets, #400 and #1200, 5 of each Spare screws and spacers for the extraction lenses Used to clean the ion lenses and the interface cones
G1820-65057 G1820-65044 G1820-65494
ICP-MS
Polishing paper kit Screw/spacer set for extraction lens
686

Electron Multiplier/Rotary Pumps
Electron Multiplier
Electron multiplier
Converts incident ions into an amplified electric current to measure the ion signal. As voltage increases, amplification factor (gain) increases.
Replace the EM when total ion G1820-80386 count reaches more than 3.2 x 1011 (this value is displayed in the software run time window), and the sensitivity is less than 4000 counts with 10 ppb Y (89 amu) even when applying detector voltage above 2700V. In general, the number of counts an EM can measure is 1.6 x 1010
Electron Multiplier P/N G1833-65420
Vacuum System: Rotary Pumps

The oil level on the pump should be checked every three months, when the plasma is ON. Maintain the oil level between the Max and Min levels on the gauge. Standard frequency for changing the oil is approximately every 4,500 hours. Warning: Be careful when changing oil because the oil may have absorbed dangerous substances. The oil in the rotary pump on the interface side of a 4500 equipped with a hydrofluoric acid resisting introduction system is especially dangerous. DO NOT TOUCH THE OIL WITH BARE HANDS.
Rotary Pump Supplies

Oil, rotary pump Oil, rotary pump Oil mist filter kit Oil mist filter element Oil mist filter kit Oil mist filter element O-ring for Vacuum Chamber, Viton Odor Element for Oil Mist Filter
4L 1L For small rotary pump RV8/RV3/E2M8 For small rotary pump RV8/RV3/E2M8 For large rotary pump, E2M18 For large rotary pump, E2M18
6040-0798 6040-0834 5063-5224 5063-5213 3162-1056 1535-4970
ICP-MS
5042-0901 For E2M18 5/pk 5063-9153
687

O-ring for vacuum release O-rings for vacuum chamber Filter kit for cooling water Tool kit
Viton O-ring Viton O-ring (one ring and one string type)
Used to seal between the vacuum chamber and its release valve Used to seal between the vacuum chamber and its cover
5/pk 2 ea.
G1820-65032 G1820-65031 G1820-65018 G1820-65017
Filter for cooling water One filter Includes: filter for cooling water, housing for the water filter, connector for the water filter, stand for the water filter, and tubing for cooling water. Includes: nut driver (1/4 in.,10 mm); hexagonal wrench (H1.5, H3, H6); flat-blade screwdriver Phillips screw-driver set #1 and #2; short Phillips screwdriver, TV screwdriver, wrench for mounting the turbo with bolts; open end wrench set (H11x13, H10, H7/16, H9/16, H4, H2.5, and H5.5); tweezers, interface wrench, Babington Nebulizer removal tool, Babington Nebulizer sample line tool. For side panel For bottom
G1820-65019
Air filter Air filter
G1820-65074 G1820-65073
ICP-MS
688
ICP-MS Standards
Installation and Checkout/Tuning Solutions/Multi-Element Calibration/Single Element
ICP-MS Standards
Installation and Checkout Standards
ICP-MS checkout solution for T-Mode. Contains detection limit solution 10 ppb Fe, Se, Pb in 0.2 wt% HNO3 ICP-MS checkout solutions for 7500s system installation kit. Contains tuning solution, dual mode (1), dual mode (2), abundance sensitivity (1), abundance sensitivity (2), detection limit solution, high sensitivity tune, wash, and water blank solutions. ICP-MS checkout solutions for 7500a, 7500i, and 7500c system installation kit. Contains tuning solution, dual mode (1), dual mode (2), wash, and water blank solutions.
5184-3563 5184-3564
5184-3565
Tuning Solutions
Stock tuning selection: Li, Y, Ce, Tl and Co; 100 mL, 10 g/mL; matrix=2% HNO3 Tuning solution: Li, Y, Ce, Tl and Co; 2 x 500 mL, 10 ng/mL; matrix=0.2% HNO3
8500-6943 5184-3566
Multi-Element Calibration Standards

Description Multi-element calibration standard-1: Ce, Dy, Er, Eu, Gd, Ho, La, Lu, Nd, Pr, Sc, Sm, Tb, Th, Tm, Y, Yb; 100 mL, 10 g/mL; matrix=5% HNO3 Multi-element calibration standard-2A: Ag, Al, As, Ba, Be, Ca, Cd, Co, Cr, Cs, Cu, Fe, Ga, Hg, K, Li, Mg, Mn, Na, Ni, Pb, Rb, Se, Sr, Tl, U, V, Zn; 100 mL, 10 g/mL: matrix=5% HNO3 Multi-element calibration standard-3: Sb, Au, Hf, Ir, Pd, Pt, Rh, Ru, Te, Sn; 100 mL, 10 g/mL; matrix=10% HCI Multi-element calibration standard-4: B, Ge, Mo, Nb, P, Re, S, Si, Ta, Ti, W, Zr; 100 mL, 10 g/mL; matrix=H20/trace HF Part No. 8500-6944 8500-6940 8500-6948 8500-6942
Single Element Standards
ICP-MS
Description Bismuth, 100 mL, 10 g/mL Cobalt, 100 mL, 10 g/mL Gold, 100 mL, 10 g/mL Indium, 100 mL, 10 g/mL Mercury, 100 mL, 10 g/mL Rhodium, 100 mL, 10 g/mL Scandium, 100 mL, 10 g/mL Terbium, 100 mL, 10 g/mL Yttrium, 100 mL, 10 g/mL
Part No. 8500-6936 8500-6947 8500-7000 8500-6946 8500-6941 8500-6945 8500-6937 8500-6938 8500-6939
689
ICP-MS Standards
Interference Check Mixes/Environmental Standards Interference Check Mixes
Description 6020 interference check mix 1: Cl, C, Ca, Fe, K, Al, Mg, Na, P, S, Mo, Ti at various concentrations; 100 mL; matrix=5% HNO3/trace HF 6020 interference check mix 2: Ag, As, Cd, Co, Cr, Cu, Mn, Ni, Zn; 100 mL, 10 g/mL, matrix=2% HNO3 Part No. 8500-6998 8500-7001
Environmental Standards
Description Environmental calibration standard: 1,000 ppm: Fe, K, Ca, Na, Mg; 10 ppm: Ag, Al, As, Ba, Be, Cd, Co, Cr, Cu, Mn, Mo, Ni, Pb, Sb, Se, Tl, V, Zn, Th, U; matrix = 10% HNO3, 100 mLs Initial calibration verification standard: 1,000 ppm: Fe, K, Ca, Na, Mg, Sr; 10 ppm: Ag, Al, As, Ba, Be, Cd, Co, Cr, Cu, Mn, Mo, Ni, Pb, Sb, Se, Tl, V, Zn, Th, U; matrix = 5% HNO3, 100 mLs Internal standard mix: 500 mLs 10 ppm: Li, Sc, Ge, Y, In, Tb, Bi; matrix = 5-10% HNO3, 500 mLs Internal standard mix: 10 ppm: Li, Sc, Ge, Y, In, Tb, Bi; matrix = 5-10% HNO3, 100 mLs Environmental spike mix: 1,000 ppm: Fe, K, Ca, Na, Mg; 100 ppm: Ag, Al, As, Ba, Be, Cd, Co, Cr, Cu, Mn, Mo, Ni, Pb, Sb, Se, Tl, V, Zn, U; matrix = 5% HNO3; 100 mLs
6 6
Part No. 5183-4688 5183-4682 5183-4680 5183-4681 5183-4687
ICP-MS
690
UV-Vis
Delivering the Highest Quality and Broadest Range

Cells and supplies are critical to the performance of your Agilent 8453 UV-Visible Spectroscopy System. Thats why Agilent is constantly working to deliver products that are proven through stringent testing to meet the highest standards, thereby ensuring that your system will operate at optimum levels. We also work to expand our range of cells, to make certain that Agilent remains your definitive supply source. This section will help you identify the cells and supplies that match your application exactly. Youll also have access to all the spectrophotometer supplies, tubing and fittings, and dissolution testing supplies you need to boost your labs productivity.

Cells
Standard cells are rectangular cells with standardized exterior dimensions. The cells either have an open top or a fused cover which can be sealed with a PTFE stopper. Some of the cells with open tops are delivered with fitted lids.
Macro Cells
The macro cell which is defined by DIN 58963 as a rectangular cell with an inner width greater than 5 mm, has emerged as the standard for photometry. The most widely used macro cell is a rectangular cell with outer dimensions of 45 x 12.5 mm (height x width). The length of the cell is dependent on the desired path length.
Semi-Micro Cells
Semi-micro and micro cells have an inner width of 4 mm to 2 mm. The thickness of the base is 9 mm. All semi-micro and micro cells are for use with spectrophotometers having a beam height of 15 mm.
Ultra-Micro Cells
These cells are specifically designed for use in the L range (down to 50 L). These cells fit into any standard cell holder and have the advantage of requiring much smaller sample volumes than standard cells. The cells are constructed so that filling and emptying can be easily accomplished with commonly available pipette tips.
UV-Vis
691
UV-Vis
Cells
Ultra-micro cells with Eppendorf pipette filling/emptying are designed to handle extremely small volumes. When only a minimum amount of sample is available, these cells provide a filling volume only slightly larger than the measuring chamber volume.
Cylindrical Cells
A cylindrical cell is a cell with plane-parallel optical surfaces whose inner volume is cylindrical in shape and has a longitudinal axis parallel to the direction of the radiation beam.
Flow-Through Cells
Until recently, flow-through cells had measuring chambers that were either rectangular or circular in shape. These shapes were dictated by limitations in the manufacturing process, and did not offer the best geometry for clean flushing and reduction of contamination. Now Agilent has developed new oval aperture cells that combine low volume with excellent flow characteristics. These oval flow cells are strongly recommended for automated analyses such as dissolution testing. Black quartz is used in the vicinity of the aperture to ensure that no light passes through the side walls of the cell.
Macro Cells
Ext. Int. Path Dimensions Dimensions Length (mm) H x W x D (mm) H x W (mm) Macro Cellswith PTFE lid 1 45 x 12.5 x 3.5 44.5 x 9.5 2
Macro Cell with PTFE Lid
Vol. (L) 350 700 1750 3500 3500 7000 17500
Part No. Glass 5063-6546
Part No. Quartz
5061-3384 45 x 12.5 x 4.5 45 x 12.5 x 7.5 45 x 12.5 x 12.5 45 x 12.5 x 12.5 44.5 x 9.5 44.5 x 9.5 44.5 x 9.5 44.5 x 9.5 44.5 x 9.5 44.5 x 9.5 5063-6547 5061-3385 5 10 10 5063-6548 5061-3386 5063-6549 5061-3387 5063-6550 1000-0544 5063-6551 5063-6553 50
Macro Cell with PTFE Stopper
Matched Pair 20 45 x 12.5 x 22.5 45 x 12.5 x 52.5
5063-6552 5063-6554
Macro Cellswith PTFE stopper
5 10
48 x 12.5 x 7.5 48 x 12.5 x 7.5
42 x 9.5 42 x 9.5
1750 3500
5063-6557 5063-6556 5062-2477
UV-Vis
Note: Spacers are required for cells with an outer depth of less than 12.5 mm to hold them securely in the cell holder.
692
UV-Vis
Cells
Semi-micro Cells
Path Length (mm) Ext. Int. Dimensions Dimensions H x W x D (mm) H x W (mm)
Vol.
(L)
Part No. Glass
Part No. Quartz
Semi-micro Cellswith PTFE lid 10 45 x 12.5 x 12.5 41.8 x 2 36 x 4 Semi-micro Cellswith PTFE stopper 10 46 x 12.5 x 12.5 37 x 4
700 1000
5061-3391 5063-6558 5063-6559

Semi-Micro Cell with PTFE Lid
1000
5063-6560 5063-6561
Helpful Hint: For applications, with a wave length range of interest in the visible range, use our low cost Agilent optical quality glass cells, made from exceptionally pure raw materials. Quartz glass gives transmission values of >80% between 200 nm and 2500 nm for an empty cell. Optical glass gives transmission values of >80% between 320 nm and 2500 nm for an empty cell.
Semi-Micro Cell with PTFE Stopper
Ultra-micro Cells
Path Ext. dimensions Aperture Center Length(mm) H x W x D (mm) H x W (mm) Height (mm)
Vol.
(L)
Filling Part No. Vol. (L) Quartz
Ultra-micro Cellswith PTFE stopper 2 45 x 12.5 x 12.5 2.5 x 2 10 45 x 12.5 x 12.5 2.5 x 2
15 15
10 50
20 70 5 10 5 10
5062-2497 5062-2496 5063-6562 5063-6563 5063-6564 5063-6565
Ultra-Micro PTFE Stopper
Ultra-micro Cellswith Eppendorf pipette filling/emptying 0.1 40 x 12.5 x 12.5 1x5 15 0.5 1 40 x 12.5 x 12.5 1x5 15 5 5 40 x 12.5 x 12.5 0.8 round 15 2.5 10 40 x 12.5 x 12.5 0.8 round 15 5
Cylindrical Cells
Ext. Dimensions Path Length (mm) H x W x D (mm) Int. Dimensions H x W (mm)
Ultra-Micro Cell with Eppendorf Filling
Vol.
(L)
Part No. Glass
Part No. Quartz
Cylindrical Cellswith PTFE stoppers 100 102.5 x 22 19
28000
5063-6566 5061-3392
Flow-through Cells
Ext. dimensions Path Length (mm) H x W x D (mm) Aperture Center Vol. Diameter (mm) Height (mm) (L) Part No. Quartz
Cylindrical Cell with PTFE Stoppers
UV-Vis
Flow-through cells with round aperture and screw fitting connection 10 35 x 12.5 x 12.5 2 15 30 0100-1224 10 35 x 12.5 x 12.5 3 15 80 0100-1225
Round Aperture Flow Cell
693
UV-Vis
Flow-through Cells
Path Ext. Dimensions Aperture Center Vol. Length (mm)H x W x D (mm) Diameter (mm) Height (mm) (L) Part No. Quartz
Flow-through cells with rectangular aperture and screw fitting connection
1
Rectangular Aperture Flow Cell
35 x 12.5 x 12.5 35 x 12.5 x 12.5 35 x 12.5 x 12.5 35 x 12.5 x 12.5
17.5 x 3.5 17.5 x 3.5 11 x 3.5 8x2
15 15 15 15
62 124 390 160
5061-3396 5061-3397 5061-3398 5062-2476
2 10 10
Flow-through cells with oval aperture and screw fitting connection
1 2
Oval Aperture Flow Cell
35 x 12.5 x 12.5 35 x 12.5 x 12.5 35 x 12.5 x 12.5 35 x 12.5 x 12.5
8x3 8x3 8x3 8x3
15 15 15 15
40 80 200 430
5063-6570 5063-6571 5063-6572 5063-6573
5 10
Note: Flow-through cells do not include tubing/fittings. See page 695 for the size of the tubing and fittings.
Cell Accessories
Spacers
Description Spacers: Spacers are required to hold cells with an outer depth of less than 12.5 mm in the cell holder. Spacer for 1 mm cell Spacer for 2 mm cell Spacer for 5 mm cell 5061-3388 5061-3389 5061-3390 Part No.
Miscellaneous
Description Magnetic stirring bar* Cell cleaning fluid Lens cleaning paper, lint free
Cell Tray
Unit 2/pk 1 Liter 50/pk 10 mm
Part No. 9301-1161 5062-8529 9300-0761 5063-6577
Cell tray for 16 cells
* For use with Agilent 89054A cell-stirring multicell transport and Agilent 89090A Peltier temperature controller. Stiffing bars are used with cells having internal dimensions of 10 x 10 mm (W x D) and cell holders with magnetic stirring capability.
UV-Vis
694
UV-Vis
Tubing and Fittings
Tubing and Fittings

Tubing and Fittings
Description Teflon tubing, 1.6 mm od Pump tubing, 2.06 mm id Pump tubing, 1.3 mm id Pump tubing, 2.8 mm id Tefzel ferrules and SS lock rings Union, polypropylene Cell fittings, black (4 short and 4 long) Conical adapter kit (connects Teflon tubing to pump tubing) Teflon nuts, for 1/16 in. od tubing Tubing, heat exchanger, FEP Peek fittings and ferrules to 8-post valve Mounting tool for flangeless nuts Unit 10 m 12/pk 12/pk 12/pk 10/pk 10/pk 8/pk 2/pk 10/pk 12 cm 10/pk Part No. 5041-2191 5041-2166 5041-2184 5041-2185 5022-2154 5022-2155 5022-2156 5022-2157 5022-2158 5042-1336 5042-1337 0100-1710
Cell Fittings (black) P/N 5022-2156 Conical Adapter Kit P/N 5022-2157 Union (10/pk) P/N 5022-2155
Teflon Nuts P/N 5022-2158
Peek Fittings and Ferrules P/N 5042-1337
UV-Vis
695
UV-Vis
Spectrophotometer Supplies
Spectrophotometer Supplies
Cell Holder Supplies
Description Optical Filter Wheel: Prevents photosensitive samples from being irradiated by UV light. Includes three filters (265 and 295 nm cut-off, and UV roll-off filter). Can be mounted on standard cell holder (P/N 08451-60104) or thermostattable cell holder (P/N 89054A). Magnetic stirring bar, 2/pk for use with 10 x 10 mm (W x D) cells Standard cell holder Part No. 08451-60302
9301-1161 08451-60104
89075C 7-Position Multicell Transport Supplies

Description Cell holder Stirring module Multicell transport adjustment tool Optical Filter Wheel: Prevents photosensitive samples from being irradiated by UV light. Includes two filters with cut-off Wavelength at 265 and 295 nm and one UV light. Part No. G1120-62301 G1120-60006 89075-23800 89075-60002
G1120A 8-Position Multicell Transport Supplies

Description Optical filter kit: Set of three optical filters to prevent photo sensitive samples from being irradiated by UV light (265 and 295 nm cut-off, and UV roll-off filter) Stirring module kit: Stirrer is driven by circulating water from water bath (not included) Magnetic stirring bar for use with 10 X 10 mm cells (W x D) Multicell transport adjustment tool Plastic cover kit 2/pk Unit Part No. G1120-68707
G1120-60006 9301-1161 89075-23800 G1120-68708
UV-Vis
696
UV-Vis
Spectrophotometer Supplies/Sipper/Lamps
89090A Peltier Temperature Controller Supplies
Description Union, cell holder (Kel-F) Flow cell, 10 mm, 8 x 2 mm aperture Quartz cuvette, 10 mm, with PTFE stopper Heat exchanging tubing for preheating of samples 160 L Unit Part No. 5021-1870 5062-2476 5062-2477 5042-1336
Autosampler Supplies
Description Needle, beveled edge for G1811A Guide, Teflon tubing for 89072A Part No. G1811-23200 1535-4878
Sipper Supplies
Description Sipper tubing kit Sipper/sampler tubing and fittings kit for 8453 Flow cell, 10 mm path length, 80 L volume Cassette, fixed pressure Cassette, variable pressure Part No. 5042-1333 5042-1334 0100-1225 5041-2167 5042-1356
Spectrophotometer Lamps
Description Deuterium lamp assembly, 8453 Tungsten lamp assembly, G1315A, 8453 Deuterium lamp assembly, 8452 Part No. 2140-0605 G1103-60001 08452-60104
UV-Vis
697
UV-Vis
Dissolution Testing Supplies
Dissolution Testing Supplies

Tubing/Fitting Kits
Description Multicell system tubing kit
Multicell Tubing Kit P/N 5042-1330
Unit
Part No. 5042-1330 5042-1331 5042-1332
Valve tubing kit for one bath Dissolution probes kit, 0.9 mm id, tubes w/fittings Peek fittings and ferrules for 8-port valve Valve, for 89079 valve unit Dissolution filters for 1/8 in. probe, 45 m pore size 1000/pk 10/pk
5042-1337 5063-6575 5181-1246
Note: See pages 693694 for additional flowcells.
Valve Tubing Kit P/N 5042-1331
UV-Vis
698
UV-Vis
Chemical Standards and Accessory Kits

Our chemical standards and accessory kits provide an inexpensive and time-saving solution for operational qualification and performance verification (OQ/PV) of UV-visible spectrophotometers. The kits are designed for analysts who need to conform closely to both quality and regulatory requirements when performing UV-visible measurements. The chemical kits can be used with any UV-visible spectrophotometer and comprise pre-prepared solutions in sealed ampoules. The solutions are those specified by the United States Pharmacopeia (USP) and European Pharmacopoeia (EP) and include holmium oxide for wavelength accuracy measurement; potassium dichromate for photometric accuracy measurement; sodium nitrite, sodium iodide and potassium chloride for stray light measurements at 340, 220, and 200 nm; and toluene in hexane for resolution measurement. Each standard includes a certificate of analysis for traceability.

Description OQ/PV Chemical Standards Kit I (for photometric accuracy, stray light and resolution measurements): Contains ten ampoules 10 mL each: 2 Dichromate, 2 Sulfuric Acid, 1 Sodium Nitrite, 1 Sodium Iodide, 1 Potassium Chloride, 1 Toluene in Hexane, 2 Hexane OQ/PV Chemical Standards Kit II (for wavelength accuracy): Contains two ampoules 10 mL each: 2 Perchloric Acid, 1 Holmium Oxide in Perchloric Acid OQ/PV Hardware Kit: Contains 2 flow cells, cell passivating fluid, tubing kit, MCT adjustment tool, temperature sensor support, syringes, and OQ/PV manual Part No. 5063-6503
5063-6521
5063-6523
Checkout Samples
Description Holmium oxide glass filter Test sample for UV-Vis (Caffeine Solution, 10 g/mL in water) Part No. 08450-60117 5063-6524
UV-Vis
699
Indices
Table of Contents
Alphabetical Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .701 Compound Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .708 Part Number Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .720
Indices
700
Alphabetical Index
A
AccuBOND II, Empty Reservoirs and Frits . . . . . . . . . . . .138 SPE Products, Bulk SPE Sorbents. . . . . . . . . . . . . . . 129 Cartridge Starter Kits. . . . . . . . . . . . . 129 Ion Exchange . . . . . . . . . . . . . . . 124125 Non-silica . . . . . . . . . . . . . . . . . . 127128 Normal Phase (Non-polar) . . . . . . . . 122 Normal Phase (Polar) . . . . . . . . 123124 Reversed Phase . . . . . . . . . . . . . 120122 AED (Atomic Emission Detector) Supplies . .196197 Agilent Cartridge Column, LC, Starter Kits . . . . . . . . . . . . . . . . . . . . . . . . .597 ALS Supplies, Miscellaneous . . . . . . . . . . . . . . . . . . . . . .173 Quadrant Trays . . . . . . . . . . . . . . . . . . . . . .173 Wash Vials . . . . . . . . . . . . . . . . . . . . . .32, 173 Amino Acid Separations, Amino Acid Standards . . . . . . . . . . . . . . .598 Application Kit . . . . . . . . . . . . . . . . . . . . . .598 Columns . . . . . . . . . . . . . . . . . . . . . . . . . . .598 Reagents . . . . . . . . . . . . . . . . . . . . . . . . . .599 Asahipak LC Columns . . . . . . . . . . . . . . . . . .570 Autosampler Supplies, ICP-MS, 4500 . . . . . . . . . . . . . . . . . . . . . . . . . . 680 7500 . . . . . . . . . . . . . . . . . . . . . . . . . . 671 UV-Vis . . . . . . . . . . . . . . . . . . . . . . . . . . . . .697 Autosampler Supplies, GC, 7673 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .173 Diffusion Caps . . . . . . . . . . . . . . . . . . . . . . .32 Purge and Trap . . . . . . . . . . . . . . . . .171172 Septum Tool . . . . . . . . . . . . . . . . . . . . . . . . .91 Syringes . . . . . . . . . . . . . . . . . . . . . . . . .4042 Wash Vials . . . . . . . . . . . . . . . . . . . . . . . . . .32 Autosampler Supplies, LC, 1050 . . . . . . . . . . . . . . . . . . . . . . . . . .487488 1050 Ti . . . . . . . . . . . . . . . . . . . . . . . . . . . .488 1090, Injector/Sampler . . . . . . . . . . . . . . . . 495 Vials, Caps . . . . . . . . . . . . . . . . . . . . . 496 1100, Metering Device . . . . . . . . . . . . . . . . 467 Needle/Seat. . . . . . . . . . . . . . . . . . . . 467 Trays . . . . . . . . . . . . . . . . . . . . . . . . . . 468 Valve Supplies . . . . . . . . . . . . . . . . . . 466 Vials, Caps . . . . . . . . . . . . . . . . . 468, 496 1100 Micro . . . . . . . . . . . . . . . . . . . . . . . . .469 1100 Micro Well Plate Sampler . . . . . . . .470 1100 Well Plate . . . . . . . . . . . . . . . . . . . . .469 220 Micro Plate Sampler . . . . . . . . . . . . .470 Pistons . . . . . . . . . . . . . . . . . . . . . . . . . . . .455
C
Cables, Instrument Interface . . . . . . . . . . . . . . . . .106 Integrator . . . . . . . . . . . . . . . . . . . . . . . . . .104 Network . . . . . . . . . . . . . . . . . . . . . . . . . . .106 Capillary Cassette, CE (Capillary Electrophoresis) . . . . . . . . .659 Capillary Column Ferrules . . . . . . . . . . .162163 Caps, Diffusion . . . . . . . . . . . . . . . . . . . . . . . . . . . .32 Fittings . . . . . . . . . . . . . . . . . . . . . . . . . . . . .83 Caps, Crimp, Headspace Vial . . . . . . . . . . . . . . . . . . . . . .34 Wide Opening . . . . . . . . . . . . . . . . . . . . . . .23 Caps, Screw, 4 mL Vial . . . . . . . . . . . . . . . . . . . . . . . . . . . .32 Standard Opening (8-425) . . . . . . . . . . . . . .30 Wide Opening . . . . . . . . . . . . . . . . . . . .1617 Caps, Snap . . . . . . . . . . . . . . . . . . . . . . . . . . . .26 Cartridge Column Fittings . . . . . . . . . . .512517 CE (Capillary Electrophoresis), PAGE Kits . . . . . . . . . . . . . . . . . . . . . . . . .660 Accessory Kit . . . . . . . . . . . . . . . . . . . . . . .659 Alignment Interfaces . . . . . . . . . . . . . . . .659 Buffers for PAGE . . . . . . . . . . . . . . . . . . .661 Buffers for CZE of Proteins . . . . . . . . . . . .654 Capillary Cassette . . . . . . . . . . . . . . . . . . .659 Capillary Conditioning Solutions . . . . . . .653 Cation Solutions Kit . . . . . . . . . . . . . . . . . .667 CE-Grade Water . . . . . . . . . . . . . . . . . . . . .653 CE-MS Supplies . . . . . . . . . . . . . . . . . . . . .657 Forensic Anions Kit . . . . . . . . . . . . . . . . . .667 High Sensitivity Detection Cell . . . . . . . . .655 Inorganic Anion Solutions Kit . . . . . . . . .666 Instrument Supplies . . . . . . . . . . . . . . . . .656 Microvials and Caps . . . . . . . . . . . . . . . . .654 Optical Filter . . . . . . . . . . . . . . . . . . . .656, 659 OQ/PV and IQ Kits . . . . . . . . . . . . . . . . . .658 Organic Acids Solutions Kit . . . . . . . . . . .667 Plating Bath Analysis Kit . . . . . . . . . . . . .668 Reagents, Buffers for CZE of Proteins . . . . . . . . 654 Conditioning Solutions . . . . . . . . . . . 653 CE-Grade Water . . . . . . . . . . . . . . . . . 653 CZE Buffers for Charged Analytes . . 654 MEKC Buffer for Neutral and Charged Analytes . . . . . . . . . 654 Standards, OQ/PV and IQ . . . . . . . . . . . . . . . . . . . 93 Start-up Kits . . . . . . . . . . . . . . . . . . . . . . . .658 Vials . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .654 Window Etching Tool . . . . . . . . . . . . . . . .659 CE Capillaries, PAGE Kits . . . . . . . . . . . . . . . . . . . . . . . . .660 SIL-DB Coated . . . . . . . . . . . . . . . . . . . . .665 SIL-WAX Coated . . . . . . . . . . . . . . . . . . .665 CE-MS . . . . . . . . . . . . . . . . . . . . . . . . . . . .657 CEP Coated . . . . . . . . . . . . . . . . . . . . . . . .664 Extended Light Path (Bubble Cell) . . . . . .662 Fused-Silica, Bulk . . . . . . . . . . . . . . . . . . .662 Packed CEC . . . . . . . . . . . . . . . . . . . . . . . .666 Packed CEC (HSDC) . . . . . . . . . . . . . . . . .666 PVA Coated . . . . . . . . . . . . . . . . . . . . . . . .663 Standard . . . . . . . . . . . . . . . . . . . . . . . . . . .661 Universal . . . . . . . . . . . . . . . . . . . . . . . . . .662 Cell Accessories, UV-Vis . . . . . . . . . . . .694696 Cell Cleaning Fluid . . . . . . . . . . . . . . . . . . . . .694 Cell Tray, UV-Vis . . . . . . . . . . . . . . . . . . . . . . .694 Cells and Cell Supplies, UV-Vis, Cylindrical . . . . . . . . . . . . . . . . . . . . . . . . .693 Flow-through . . . . . . . . . . . . . . . . . . .693694
B
Bar Code Reader Labels . . . . . . . . . . . . . . . .173 Bleed and Temperature Optimized Septa . .151 Books, Capillary Electrophoresis Primer . . . . . . .109 Gas Chromatography . . . . . . . . . . . . . . . .108 General Chromatography . . . . . . . . . . . . .108 Good Laboratory Practice Primer . . . . . . .109 Liquid Chromatography . . . . . . . . . . . . . . .109 Buffers, CE, For CZE of Proteins . . . . . . . . . . . . . . . . . .654 Buffers for PAGE . . . . . . . . . . . . . . . . . . .661 For CZE of Charged Analytes . . . . . . . . . .654 MEKC, for Neutral and Charged Analytes . .654
Macro . . . . . . . . . . . . . . . . . . . . . . . . . . . . .692 Semi-micro . . . . . . . . . . . . . . . . . . . . . . . . .693 Ultra-micro . . . . . . . . . . . . . . . . . . . . . . . . .693 CE-MS, Alignment Interface . . . . . . . . . . . . . . . . .659 Capillaries . . . . . . . . . . . . . . . . . . . . . . . . .657 CE-MS Adapter Kit . . . . . . . . . . . . . . . . . .657 Nebulizer Kit . . . . . . . . . . . . . . . . . . . . . . .657 Ceramic Wafer, GC Column Cutter . . . . .88, 314 ChiraDex LC Columns . . . . . . . . . . . . . . . . . .577 Chiral LC Columns, ChiraDex . . . . . . . . . . . . . . . . . . . . . . . . . . .577 Ultron . . . . . . . . . . . . . . . . . . . . . . . . . . . . .576 ChromTrac Tubing Labels . . . . . . . . . . . . . . .447 Cleaning and Maintenance Supplies, MS . . . . . . . . . . . . . . . . . . . . . . . . . . .205206 MS Engine . . . . . . . . . . . . . . . . . . . . . . . . .214 Nylon Gloves . . . . . . . . . . . . . . . . . . . . . . .202 Column Compartment Supplies, LC, 1090, Switching Valve . . . . . . . . . . . . . . . . . 497 1100, Capillary Column Switching Valve . . 471 Column Clamp . . . . . . . . . . . . . . . . . . 471 Column ID Module. . . . . . . . . . . . . . . 471 Column Switching Valve . . . . . . . . . . 471 Connecting Capillaries . . . . . . . . . . . 471 Temp Calibration Kit . . . . . . . . . . . . . 471 Column Connectors, GC, Glass . . . . . . . . . . . . . . . . . . . . . . . . .164165 Graphpak . . . . . . . . . . . . . . . . . . . . . .165166 Metal . . . . . . . . . . . . . . . . . . . . . . . . . . . . .165 Quartz . . . . . . . . . . . . . . . . . . . . . . . . . . . . .165 Column Cutting Tool with Rotating Diamond . . .88 Column Installation Tool Kit . . . . . . . . . .88, 314 Column Nuts, GC, Finger-tight . . . . . . . . . . . . . . . . . . . . . . . . .163 GC/MS (Long) . . . . . . . . . . . . . . . . . .163, 201 Standard (Short) . . . . . . . . . . . . . . . . . . . .163 CombiHT Columns . . . . . . . . . . . . . . . . . . . . .553 CombiZorb Scavengers . . . . . . . . . . . . . . . . .141 Connectors, ICP-MS, 4500 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .679 7500 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .670 Connectors (Swagelok-style To NPT) . . . .85 Cool on-Column Inlet Supplies, 5890 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .190 6890 . . . . . . . . . . . . . . . . . . . . . . . . . .178179 Crimp Caps, Headspace Vial (20 mm) . . . . . . . . . . . . . . .34 Wide Opening . . . . . . . . . . . . . . . . . . . . . . .23 Crimp Top Vials, Kits and Convenience Packs . . . . . . . . . . .23 Wide Opening, Extreme Recovery . . . . . . . . . . . . . . . . 22 Glass . . . . . . . . . . . . . . . . . . . . . . . . . . . 20 Glass, Deactivated. . . . . . . . . . . . . . . . 21 Glass-lined Plastic . . . . . . . . . . . . 22, 26 High Recovery, Micro-V Vial . . . . . . . . 21 Polypropylene. . . . . . . . . . . . . . . . . 22, 25 Crimper, Electronic . . . . . . . . . . . . . . . . . . . . . . . . . . .37 Manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . .38 Crosses (Fittings) . . . . . . . . . . . . . . . . . . . . . . .84 Cuvettes . . . . . . . . . . . . . . . . . . . . . . . . .692694 Cylinder Bracket . . . . . . . . . . . . . . . . . . . . . . .82 CZE Buffers, For Charged Analytes . . . . . . . . . . . . . . . .654 For Proteins . . . . . . . . . . . . . . . . . . . . . . . .654
Indices
701
Alphabetical Index
D
DB-EVDX, GC Columns . . . . . . . . . . . . . . . . .131 Decapper, Electronic . . . . . . . . . . . . . . . . . . . . . . . . . . .37 Manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . .38 Detector Supplies, GC, AED . . . . . . . . . . . . . . . . . . . . . . . . . .196197 ECD, 5890 . . . . . . . . . . . . . . . . . . . . . . . . . . 194 6890 . . . . . . . . . . . . . . . . . . . . . . . . . . 187 FID, 5890 . . . . . . . . . . . . . . . . . . . . . . 192193 6890/6850 . . . . . . . . . . . . . . . . . 182183 FPD, 5890 . . . . . . . . . . . . . . . . . . . . . . . . . . 195 6890 . . . . . . . . . . . . . . . . . . . . . . . . . . 187 NPD, 5890 . . . . . . . . . . . . . . . . . . . . . . 192193 6890 . . . . . . . . . . . . . . . . . . . . . . 184185 TCD, 5890 . . . . . . . . . . . . . . . . . . . . . . . . . . 194 6890/6850 . . . . . . . . . . . . . . . . . 185186 Test Samples . . . . . . . . . . . . . . . . . . . . . . . .98 Tools . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .91 Detector Supplies, LC, 1050, DAD/MWD Supplies. . . . . . . . . . . . . 491 VWD HP/UHP Flow Cells . . . . . . . . . 490 VWD Micro Flow Cell . . . . . . . . . . . . 490 VWD Prep Flow Cell . . . . . . . . . . . . . 490 VWD Standard Flow Cells. . . . . . . . . 489 VWD Supplies . . . . . . . . . . . . . . . . . . 489 1090, DAD Series I/II . . . . . . . . . . . . . . . . . 498 Filter Photometric (FPD) . . . . . . . . . . 497 1100, DAD 500 nL Flow Cell . . . . . . . . . . . . 480 DAD Flow Cells . . . . . . . . . . . . . . . . . 477 DAD Micro/HP Flow Cell . . . . . . . . . 479 DAD Semi-Micro Flow Cell . . . . . . . . 479 DAD Standard Flow Cell . . . . . . . . . . 478 DAD Supplies . . . . . . . . . . . . . . . . . . . 478 DAD/MWD Prep Cells . . . . . . . . . . . 481 Fluorescence . . . . . . . . . . . . . . . . . . . 481 Refractive Index . . . . . . . . . . . . . . . . . 482 VWD Flow Cells . . . . . . . . . . . . . . . . . 474 VWD High Press Flow Cell . . . . . . . . 476 VWD Micro Flow Cell . . . . . . . . . . . . 476 VWD Semi-Micro Flow Cell . . . . . . . 476 VWD Standard Flow Cells. . . . . . . . . 475 VWD Supplies . . . . . . . . . . . . . . . . . . 474 1037/47 Refractive Index . . . . . . . . . . . . .499 1040 DAD Series I/II . . . . . . . . . . . . . . . . .499 1046 Fluorescence . . . . . . . . . . . . . .499500 1049 Electrochemical . . . . . . . . . . . . . . . .500 Flow Cells . . . . . . . . . . . . . . . . . . . . . .456, 477 Lamps . . . . . . . . . . . . . . . . . . . . . . . . .455, 474 Detector Supplies, MS . . . . . . . . . . . . .201215 Diamond-tipped Column Cutting Pencil . . . . . .88 Diffusion Caps . . . . . . . . . . . . . . . . . . . . . . . . .32 Diffusion Pump Supplies, Ion Gauge Supplies . . . . . . . . . . . . . . . . . .209 Pump Oil . . . . . . . . . . . . . . . . . . . . . . . . . . .209 Dissolution Testing Supplies . . . . . . . . . . . .698 Electron Multipliers, ICP-MS, 4500 . . . . . . . . . . . . . . . . . . . . . . . . . . 687 7500 . . . . . . . . . . . . . . . . . . . . . . . . . . 678 MS, MSD . . . . . . . . . . . . . . . . . . . . . .210, 215 Electronic Crimpers & Decappers . . . . . . . . .37 EPA 8270 Application Kit . . . . . . . . . . . . . . .212 Evidex II SPE Products . . . . . . . . . . . . . . . . .130 Fused Silica Tubing, Deactivated . . . . . . . . . . . . . . . . . . . . . . . .311 Deactivated, High Temp . . . . . . . . . . . . . .312 Undeactivated . . . . . . . . . . . . . . . . . . . . . .313
G
Gas Analyzer Accessories & Standards . .100101 Gas Line Supplies, ICP-MS, 4500 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .679 7500 . . . . . . . . . . . . . . . . . . . . . . . . . .669670 Gas Management, Cylinder Bracket . . . . . . . . . . . . . . . . . . . . .82 Flowmeters . . . . . . . . . . . . . . . . . . . . . .7579 Gas Regulators, Brass, CGA . . . . . . . . . . . . . . . . . . . . . . 81 Outlet Adapters . . . . . . . . . . . . . . . 81, 85 GC Installation Kits . . . . . . . . . . . . . . . .7172 Leak Detector . . . . . . . . . . . . . . . . . . . . . . .80 Tubing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .82 Gas Regulators, 5890/6890 Instruments . . . . . . . . . . . . . .198 Brass, CGA . . . . . . . . . . . . . . . . . . . . . . . . . .81 Outlet Adapters . . . . . . . . . . . . . . . . . . .81, 85 Gas Sampling Valves . . . . . . . . . . . . . . . . . .167 Gas Traps, A Selection Guide . . . . . . . . . . . . . . . . .4849 Combination Traps, Big Universal (RMSH Series) . . . . . . . 62 Gas Specific for MS. . . . . . . . . . . . 6162 Hydrocarbon/Moisture Removal . . . . 61 Oxygen/Moisture Removal . . . . . 6061 Gas Purification Systems, High Capacity. . . . . . . . . . . . . . . . . 6364 Point of Operation Panels. . . . . . . 6567 Super Clean . . . . . . . . . . . . . . . . . . 6869 GC Instrument, Detector, 5890 . . . . . . . . . . . . . . . . . . . 70 Internal Split Vent, 5890 . . . . . . . . . . . 70 Split Vent Trap . . . . . . . . . . . . . . . . . . . 70 Hydrocarbon Removal . . . . . . . . . . . . . .5758 Moisture Removal . . . . . . . . . . . . . . . .5155 Oxygen Removal . . . . . . . . . . . . . . . . . .5657 Trap Mounting Panel/Clips . . . . . . . . . . . .70 GC Buddy Multi-purpose Tool . . . . . . . . . . . . .91 GC Column Cutting Tools . . . . . . . . . . . .88, 314 GC Column Rinse Kit . . . . . . . . . . . . . . . .88, 314 GC Columns, 6850 on 5 in. Cage, CAM . . . . . . . . . . . . . . . . . . . . . . . . . . 300 DB-1 . . . . . . . . . . . . . . . . . . . . . . . . . . 300 DB-1301 . . . . . . . . . . . . . . . . . . . . . . . 301 DB-17 . . . . . . . . . . . . . . . . . . . . . . . . . 301 DB-1701 . . . . . . . . . . . . . . . . . . . . . . . 301 DB-1701P . . . . . . . . . . . . . . . . . . . . . . 302 DB-1ms. . . . . . . . . . . . . . . . . . . . . . . . 300 DB-225ms. . . . . . . . . . . . . . . . . . . . . . 301 DB-5 . . . . . . . . . . . . . . . . . . . . . . . . . . 301 DB-5ms. . . . . . . . . . . . . . . . . . . . . . . . 301 DB-624 . . . . . . . . . . . . . . . . . . . . . . . . 301 DB-FFAP . . . . . . . . . . . . . . . . . . . . . . . 302 DB-MTBE . . . . . . . . . . . . . . . . . . . . . . 302 DB-Petro. . . . . . . . . . . . . . . . . . . . . . . 302 DB-PS1 . . . . . . . . . . . . . . . . . . . . . . . . 302 DB-VRX . . . . . . . . . . . . . . . . . . . . . . . . 302 DB-WAX . . . . . . . . . . . . . . . . . . . . . . . 302 GS-Q . . . . . . . . . . . . . . . . . . . . . . . . . . 302 HP Blood Alcohol Analysis . . . . . . . . 304 HP Fast GC Residual Solvents . . . . . 304 HP-1 . . . . . . . . . . . . . . . . . . . . . . . . . . 303 HP-101 . . . . . . . . . . . . . . . . . . . . . . . . 304 HP-1ms . . . . . . . . . . . . . . . . . . . . . . . . 303 HP-5 . . . . . . . . . . . . . . . . . . . . . . . . . . 304 HP-50+ . . . . . . . . . . . . . . . . . . . . . . . . 304
F
Ferrule Tools, GC Column . . . . . . . . . . . .89, 314 Ferrules, Capillary Column . . . . . . . . . . . . . . . . . . .162 GC Inlet Liner . . . . . . . . . . . . . . . . . . . . . . .159 Metal (1/16, 1/8, 1/4 in.) . . . . . . . . . . . . . .83 Reducing . . . . . . . . . . . . . . . . . . . . . . . . . .163 Straight . . . . . . . . . . . . . . . . . . . . . . . . . . . .163 TCD . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .186 FID Supplies, 5890 . . . . . . . . . . . . . . . . . . . . . . . . . .192193 6890/6850 . . . . . . . . . . . . . . . . . . . . .182183 Cleaning Kits . . . . . . . . . . . . . . . . . . . .90, 182 Igniter Cable (6890/6850) . . . . . . . . . . . . .183 Jets (6890/6850) . . . . . . . . . . . . . . . .182183 Test Samples . . . . . . . . . . . . . . . . . . . .98, 182 Filaments, 5989 MS Engine . . . . . . . . . . . . . . . . . . . .214 MSD . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .207 File Set, 12-Piece . . . . . . . . . . . . . . . . . . . . . .90 Filters, Chemical, 5890 Internal . . . . . . . . . . . . . . .70 ICP-MS Cooling Water, 4500 . . . . . . . . . . . . . . . . . . . . . . . . . . 688 7500 . . . . . . . . . . . . . . . . . . . . . . . . . . 678 Syringe . . . . . . . . . . . . . . . . . . . . . . . .146147 Fittings, Caps . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .83 Connectors (Swagelok-style To NPT) . . . . .85 Crosses . . . . . . . . . . . . . . . . . . . . . . . . . . . . .84 Elbows . . . . . . . . . . . . . . . . . . . . . . . . . . . . .84 Ferrules . . . . . . . . . . . . . . . . . . . . . . . . . . . .83 General Purpose Kit . . . . . . . . . . . . . . . . . .85 Miscellaneous Parts . . . . . . . . . . . . . . . . . .83 Nuts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .83 Plugs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .83 Tees . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .84 Unions . . . . . . . . . . . . . . . . . . . . . . . . . . . . .84 Unions, Reducing . . . . . . . . . . . . . . . . . . . .85 Fittings, LC, Capillary LC Fittings . . . . . . . . . . . . . . . . .473 PEEK . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .447 Stainless Steel . . . . . . . . . . . . . . . . . . . . . .447 Flexible Tubing, LC . . . . . . . . .445446, 459, 486 Flow Cells, LC . . . . . . . . . . . . . . . . . . . . . . . . .456 Flow Tracker Flowmeter . . . . . . . . . . . . . . . . .75 Flowmeter/Leak Detector . . . . . . . . . . . . . . . .75 Flowmeters, Gas, A Selection Guide . . . . . . . . . . . . . . . . . . . .74 ADM . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .77 Digital Bubble . . . . . . . . . . . . . . . . . . . . . . .79 FlowTracker . . . . . . . . . . . . . . . . . . . . . . . . .75 Glass Bubble . . . . . . . . . . . . . . . . . . . . . . . .79 Optiflow . . . . . . . . . . . . . . . . . . . . . . . . . . . .79 Veri-Flow 500 . . . . . . . . . . . . . . . . . . . . . . . .77 Flowmeters, Liquid . . . . . . . . . . . . . . . . . . . . .79 Forceps . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .87 FPD Supplies, 5890 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .195 6890 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .187 Fraction Collector Supplies, 1100 . . . . . . . . .465 Frits, LC Column . . . . . . . . . . . . . . . . . . . . . . .518
Indices
E
ECD Supplies, 5890 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .194 6890 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .187 Test Samples . . . . . . . . . . . . . . . . . . . . . . . .98 Elbows (Fittings) . . . . . . . . . . . . . . . . . . . . . . .84
702
Alphabetical Index
HP-5ms . . . . . . . . . . . . . . . . . . . . . . . . 304 HP-FFAP . . . . . . . . . . . . . . . . . . . . . . . 305 HP-INNOWax . . . . . . . . . . . . . . . . . . . 305 HP-PLOT Al2O3 KCl . . . . . . . . . . . . 305 HP-PLOT Al2O3 M . . . . . . . . . . . . . 305 HP-PLOT Al2O3 S. . . . . . . . . . . . . . 305 HP-PLOT Molesieve. . . . . . . . . . . . . . 305 HP-PLOT Q . . . . . . . . . . . . . . . . . . . . . 306 HP-PLOT U . . . . . . . . . . . . . . . . . . . . . 306 HP-PONA . . . . . . . . . . . . . . . . . . . . . . 306 Ultra 1 . . . . . . . . . . . . . . . . . . . . . . . . . 306 Ultra 2 . . . . . . . . . . . . . . . . . . . . . . . . . 306 Cage only, 5 in. (For 6850) . . . . . . . . . . . .306 Columns on 8 in. Cage, CAM . . . . . . . . . . . . . . . . . . . . . . . . . . 296 Carbowax 20M . . . . . . . . . . . . . . . . . . 295 Column Installation Kit . . . . . . . . . . . . 88 Column Rinse Kit . . . . . . . . . . . . . . . . . 88 Cyclodex-B . . . . . . . . . . . . . . . . . . . . . 268 CycloSil-B . . . . . . . . . . . . . . . . . . . . . . 267 DB-1 . . . . . . . . . . . . . . . . . . . . . . 232235 DB-1301 . . . . . . . . . . . . . . . . . . . . . . . 258 DB-17 . . . . . . . . . . . . . . . . . . . . . 251252 DB-17/DB-1701P, Dual Column . . . . 276 DB-1701 . . . . . . . . . . . . . . . . . . . . . . . 259 DB-1701P . . . . . . . . . . . . . . . . . . . . . . 275 DB-17-EVDX . . . . . . . . . . . . . . . . 131, 273 DB-17ht. . . . . . . . . . . . . . . . . . . . . . . . 271 DB-17ms. . . . . . . . . . . . . . . . . . . 229, 253 DB-1-EVDX . . . . . . . . . . . . . . . . . 131, 273 DB-1ht. . . . . . . . . . . . . . . . . . . . . . . . . 270 DB-1ms. . . . . . . . . . . . . . . . 221222, 237 DB-200 . . . . . . . . . . . . . . . . . . . . . . . . 255 DB-210 . . . . . . . . . . . . . . . . . . . . . . . . 255 DB-225 . . . . . . . . . . . . . . . . . . . . . . . . 256 DB-225ms. . . . . . . . . . . . . . . . . . 230, 257 DB-23 . . . . . . . . . . . . . . . . . . . . . . . . . 254 DB-2887 . . . . . . . . . . . . . . . . . . . . . . . 277 DB-35 . . . . . . . . . . . . . . . . . . . . . . . . . 248 DB-35ms. . . . . . . . . . . . . . . . . . . 228, 250 DB-5 . . . . . . . . . . . . . . . . . . . . . . 239241 DB-5.625 . . . . . . . . . . . . . . . . . . . . . . . 281 DB-5/DB-17, Dual Column . . . . . . . . 276 DB-5/DB-1701P, Dual Column . . . . . 276 DB-502.2 . . . . . . . . . . . . . . . . . . . . . . . 284 DB-5ht. . . . . . . . . . . . . . . . . . . . . . . . . 271 DB-5ms . . . . . . . . . . . 223224, 243244 DB-5ms-EVDX . . . . . . . . . . . . . . 131, 273 DB-608 . . . . . . . . . . . . . . . . . . . . . . . . 275 DB-624 . . . . . . . . . . . . . . . . . . . . 257, 283 DB-ALC1 . . . . . . . . . . . . . . . . . . . . . . . 272 DB-ALC2 . . . . . . . . . . . . . . . . . . . . . . . 272 DB-Dioxin . . . . . . . . . . . . . . . . . . . . . . 280 DB-EVDX . . . . . . . . . . . . . . . . . . 131, 273 DB-FFAP . . . . . . . . . . . . . . . . . . . 265266 DB-HT SimDis . . . . . . . . . . . . . . . . . . 278 DB-MTBE . . . . . . . . . . . . . . . . . . . . . . 285 DB-Petro. . . . . . . . . . . . . . . . . . . . . . . 279 DB-ProSteel . . . . . . . . . . . . . . . . . . . . 293 DB-PS1 . . . . . . . . . . . . . . . . . . . . . . . . 293 DB-PS2887 . . . . . . . . . . . . . . . . . . . . . 293 DB-PS5 . . . . . . . . . . . . . . . . . . . . . . . . 293 DB-PS624 . . . . . . . . . . . . . . . . . . . . . . 293 DB-PSWAX. . . . . . . . . . . . . . . . . . . . . 293 DB-TPH . . . . . . . . . . . . . . . . . . . . . . . . 285 DB-VRX. . . . . . . . . . . . . . . . . . . . 282283 DB-WAX . . . . . . . . . . . . . . . . . . . 261262 DB-WAXetr. . . . . . . . . . . . . . . . . . . . . 263 DB-XLB . . . . . . . . . . . . . . . . . . . . 227, 247 Drugs of Abuse . . . . . . . . . . . . . 131, 273 Dual Columns. . . . . . . . . . . . . . . . . . . 276 DX-1, DX-3, DX-4 . . . . . . . . . . . . . . . . 297 GS-Alumina . . . . . . . . . . . . . . . . . . . . 289 GS-Alumina KCl . . . . . . . . . . . . . . . . . 287 GS-CarbonPLOT . . . . . . . . . . . . . . . . . 290 GS-GasPro . . . . . . . . . . . . . . . . . . . . . 289 GS-Q . . . . . . . . . . . . . . . . . . . . . . . . . . 292 HP Blood Alcohol Analysis . . . . . . . . 273 HP Fast Residual Solvents . . . . . . . . 273 HP PAS 5 . . . . . . . . . . . . . . . . . . . . . . 275 HP PLOT Al2O3 KCl . . . . . . . . . . . . 287 HP PLOT Al2O3 M . . . . . . . . . . . . . 289 HP PLOT Al2O3 S. . . . . . . . . . . . . . 288 HP-1 . . . . . . . . . . . . . . . . . . . . . . 235237 HP-101 . . . . . . . . . . . . . . . . . . . . . . . . 294 HP-17 . . . . . . . . . . . . . . . . . . . . . . . . . 294 HP-1ms . . . . . . . . . . . . . . . . . . . . 222, 238 HP-20M. . . . . . . . . . . . . . . . . . . . . . . . 295 HP-35 . . . . . . . . . . . . . . . . . . . . . . . . . 249 HP-5 . . . . . . . . . . . . . . . . . . . . . . . . . . 242 HP-50+ . . . . . . . . . . . . . . . . . . . . 252253 HP-5ms . . . . . . . . . . . . . . . . 225226, 245 HP-Chiral-10 . . . . . . . . . . . . . . . . . . . 268 HP-Chiral-20 . . . . . . . . . . . . . . . . . . . 268 HP-FFAP . . . . . . . . . . . . . . . . . . . . . . . 266 HP-INNOWax . . . . . . . . . . . . . . . 264265 HP-PLOT MoleSieve . . . . . . . . . . . . . 290 HP-PLOT Q . . . . . . . . . . . . . . . . . . . . . 291 HP-PLOT U . . . . . . . . . . . . . . . . . . . . . 292 HP-PONA . . . . . . . . . . . . . . . . . . . . . . 279 HP-Ultra 1. . . . . . . . . . . . . . . . . . 269270 HP-Ultra 2 . . . . . . . . . . . . . . . . . . . . . . 270 HP-VOC . . . . . . . . . . . . . . . . . . . . . . . . 284 OV-351 . . . . . . . . . . . . . . . . . . . . . . . . 295 SE-30, SE-54 . . . . . . . . . . . . . . . . . . . . 298 Fused Silica Tubing, Deactivated . . . . . . . . . . . . . . . . . . . . 311 Deactivated, High Temp . . . . . . . . . . 312 Undeactivated . . . . . . . . . . . . . . . . . . 313 Installation Tools . . . . . . . . . . . . . . . . . .8889 Particle Traps . . . . . . . . . . . . . . . . . . . . . . .291 Quality Control Specs, DB-1 . . . . . . . . . . . . . . . . . . . . . . . . . . 307 DB-17 . . . . . . . . . . . . . . . . . . . . . . . . . 309 DB-17ms. . . . . . . . . . . . . . . . . . . . . . . 310 DB-1ms. . . . . . . . . . . . . . . . . . . . . . . . 308 DB-200 . . . . . . . . . . . . . . . . . . . . . . . . 310 DB-225ms. . . . . . . . . . . . . . . . . . . . . . 310 DB-35ms. . . . . . . . . . . . . . . . . . . . . . . 310 DB-5 . . . . . . . . . . . . . . . . . . . . . . . . . . 308 DB-5ms. . . . . . . . . . . . . . . . . . . . 308310 DB-WAX . . . . . . . . . . . . . . . . . . . . . . . 309 DB-XLB . . . . . . . . . . . . . . . . . . . . . . . . 310 Retention Gaps . . . . . . . . . . . . . . . . . . . . .311 Stainless Steel Tubing (ProSteel), Deactivated . . . . . . . . . . . . . . . . . . . . 312 Undeactivated . . . . . . . . . . . . . . . . . . 313 Test Standards . . . . . . . . . . . . . . . . . . . .9697 Tools, Ceramic Wafer Cutter . . . . . . . . . . . . 314 Column Rinse Kit . . . . . . . . . . . . . . . . 314 Installation Tools . . . . . . . . . . . . . . . . 314 Tubing Cutters . . . . . . . . . . . . . . . . . . . . . . .88 GC Installation Kits . . . . . . . . . . . . . . . . . .7172 Gel Filtration LC Columns, PLgel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .584 ZORBAX GF-250 . . . . . . . . . . . . . . . . . . . .585 ZORBAX GF-450 . . . . . . . . . . . . . . . . . . . .585 ZORBAX PSM . . . . . . . . . . . . . . . . . . . . . .581 GFC Calibration Standards . . . . . . . . . . . . . . .92 Gloves, Lint Free . . . . . . . . . . . . . . . . . . . . . .202 GPC, GFC Calibration Standards . . . . . . . . . . .92 GPC-SEC, Calibration Standards . . . . . . . . . . . . . . . .579 PLgel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .584 Start-up Kits . . . . . . . . . . . . . . . . . . . . . . . .578 ZORBAX GF-250 . . . . . . . . . . . . . . . . . . . .585 ZORBAX GF-450 . . . . . . . . . . . . . . . . . . . .585 ZORBAX PSM . . . . . . . . . . . . . . . . . . . . . .581 Graphpak Capillary Column Connectors . . .165166 Guard Cartridge Holders, LC Columns . . .512517
H
Headspace Sampler Supplies . . . . . . . .174175 Headspace Vials, Caps, Septa . . . . . . . . .3335 Hex Keys . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .87 High Sensitivity Detection Cell, CE . . . . . . .655 Hypersil Cartridge Columns, BDS-C18 . . . . . . . . . . . . . . . . . . . . . . . . . . .566 BDS-C18 (CIM) . . . . . . . . . . . . . . . . .565566 BDS-C8 . . . . . . . . . . . . . . . . . . . . . . . . . . . .566 Guards . . . . . . . . . . . . . . . . . . . . . . . . . . . .566 ODS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .566 ODS (CIM) . . . . . . . . . . . . . . . . . . . . .565566 Hypersil Columns, BDS-C18 . . . . . . . . . . . . . . . . . . . . . . . . . . .567 Guards . . . . . . . . . . . . . . . . . . . . . . . . . . . .567 HPLC Capillary . . . . . . . . . . . . . . . . . . . . . .567 MOS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .567 Normal Phase . . . . . . . . . . . . . . . . . . . . . .575 ODS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .567 ODS (CIM) . . . . . . . . . . . . . . . . . . . . .565, 567
I
ICP-MS Supplies, 4500, Autosampler Supplies . . . . . . . . . . . . 680 Connectors . . . . . . . . . . . . . . . . . . . . . 679 Electron Multiplier . . . . . . . . . . . . . . . 687 Gas Line Supplies . . . . . . . . . . . . . . . 679 Ion Lens System Components . . . . . 686 ISIS Supplies . . . . . . . . . . . . . . . . . . . 681 Nebulizers & Supplies. . . . . . . . 682683 Peristaltic Pump Tubing . . . . . . . . . . 680 Sampling Cones . . . . . . . . . . . . 685686 Skimmer Cones . . . . . . . . . . . . . 685686 Spray Chambers. . . . . . . . . . . . . 683684 Torches & Components. . . . . . . 684685 Vacuum System, Misc. . . . . . . . 687688 7500, Autosampler Supplies . . . . . . . . . . . . 671 Connectors . . . . . . . . . . . . . . . . . . . . . 670 Electron Multiplier . . . . . . . . . . . . . . . 678 Gas Line Supplies . . . . . . . . . . . . . . . 669 Ion Lens System Components . . . . . 677 ISIS Supplies . . . . . . . . . . . . . . . 671672 Nebulizers & Supplies. . . . . . . . 673674 Peristaltic Pump Tubing . . . . . . . . . . 670 Sampling Cones . . . . . . . . . . . . . . . . 676 Skimmer Cones . . . . . . . . . . . . . . . . . 676 Spray Chambers. . . . . . . . . . . . . . . . . 674 Torches & Components . . . . . . . . . . . 675 Vacuum System, Misc. . . . . . . . . . . . 678 Vials . . . . . . . . . . . . . . . . . . . . . . . . . . 671 Standards . . . . . . . . . . . . . . . . . . . . . .689690 Igniter Cable, FID . . . . . . . . . . . . . . . . . . . . . .183 Injector Valve Syringes . . . . . . . . . . . . . .46, 454 Inlet Liners, Direct . . . . . . . . . . . . . . . . . . . . . . . . . . . . .157 Direct Connect . . . . . . . . . . . . . . . . . . . . . .158 Focus . . . . . . . . . . . . . . . . . . . . . . . . . . . . .158 Liner O-Rings . . . . . . . . . . . . . . . . . . . . . . .158 Megabore . . . . . . . . . . . . . . . . . . . . . . . . . .159 Megaliner . . . . . . . . . . . . . . . . . . . . . . . . . .158 Split/Splitless . . . . . . . . . . . . . . . . . . . . . .157 Inlet Supplies, Cleaning Wires . . . . . . . . . . . . . . . . . . . . . .91 Column Nuts . . . . . . . . . . . . . . . . . . . . . . .163 Cool on-Column, 5890 . . . . . . . . . . . . . . . . . . . . . . . . . . 190 6890 . . . . . . . . . . . . . . . . . . . . . . 178179 Evaluation Sample . . . . . . . . . . . . . . . . . . . .98
Indices
703
Alphabetical Index
Inlet Supplies continued, Packed Column Inlet, 5890 . . . . . . . . . . . . . . . . . . . . . . . . . . 191 6890/6850 . . . . . . . . . . . . . . . . . . . . . 180 PTV (Programmable Temperature Vaporizing), 6890 . . . . . . . . . . . . . . . . . . . . . . . . . . 177 Split/Splitless, 5890 . . . . . . . . . . . . . . . . . . . . . . 188189 6890/6850 . . . . . . . . . . . . . . . . . 175176 Inlet Tools, GC . . . . . . . . . . . . . . . . . . . . . . . . .89 Inserts for Vials, For Standard Opening (8-425) Vials . . . . . .31 For Wide Opening Vials . . . . . . . . . . . . . . .28 Integrated Sample Introduction System Supplies, ICP-MS, 4500 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .681 7500 . . . . . . . . . . . . . . . . . . . . . . . . . .671672 Integrator Supplies, Accessories . . . . . . . . . . . . . . . . . . . . . . . .104 Cables . . . . . . . . . . . . . . . . . . . . . . . .104106 Cartridges and Printheads . . . . . . . . . . . .102 Paper and Supplies . . . . . . . . . . . . . .102103 Integrators . . . . . . . . . . . . . . . . . . . . . . . . . . .104 Ion Lens System Components, ICP-MS, 4500 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .686 7500 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .677 7500c . . . . . . . . . . . . . . . . . . . . . . . . . . . . .677 Ion Source Parts and Supplies, 5973 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .206 5972/5971/GCD MSD . . . . . . . . . . . . . . . .207 MS Engine . . . . . . . . . . . . . . . . . . . .214215 ODS (CIM) . . . . . . . . . . . . . . . . . 565, 567 Hypersil (Cartridge), BDS-C18 . . . . . . . . . . . . . . . . . . . . . . . 566 BDS-C18 (CIM). . . . . . . . . . . . . . . . . . 565 BDS-C8 . . . . . . . . . . . . . . . . . . . . . . . . 566 Guards . . . . . . . . . . . . . . . . . . . . . . . . 566 ODS . . . . . . . . . . . . . . . . . . . . . . . . . . . 566 ODS (CIM) . . . . . . . . . . . . . . . . . 565566 LiChrosorb . . . . . . . . . . . . . . . . . . . . . . . . .569 LiChrospher, CN . . . . . . . . . . . . . . . . . . . . . . . . . . . . 568 Normal Phase . . . . . . . . . . . . . . . . . . 575 RP-18. . . . . . . . . . . . . . . . . . . . . . . . . . 568 RP-18 Endcapped. . . . . . . . . . . . . . . . 568 RP-8. . . . . . . . . . . . . . . . . . . . . . . . . . . 568 RP-Select B . . . . . . . . . . . . . . . . . . . . 568 Nucleosil . . . . . . . . . . . . . . . . . . . . . . . . . .569 PAH Columns . . . . . . . . . . . . . . . . . . . . . . .599 Pesticide Column . . . . . . . . . . . . . . . . . . .599 PL Aquagel-OH . . . . . . . . . . . . . . . . . . . . .588 PLgel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .584 PTH Columns . . . . . . . . . . . . . . . . . . . . . . .599 Purospher . . . . . . . . . . . . . . . . . . . . . . . . . .570 Sample Cleanup Column Kits . . . . . . . . . .518 Special Applications, Amino Acid Separations . . . . . . . . . . 599 Anion Chromatography . . . . . . . . . . . 599 Superspher . . . . . . . . . . . . . . . . . . . . . . . . .571 SynChropak, Hydrophobic Interaction . . . . . . . . . . 591 Ion Exchange . . . . . . . . . . . . . . . . . . . 591 Wide-Pore . . . . . . . . . . . . . . . . . . . . . 572 Ultron . . . . . . . . . . . . . . . . . . . . . . . . . . . . .576 Vydac, PAH Columns . . . . . . . . . . . . . . . . . . . 599 Wide-Pore . . . . . . . . . . . . . . . . . . . . . 572 ZORBAX, Bonus-RP . . . . . . . . . . . . . . . . . . . . . . 543 Carbohydrate Analysis . . . . . . . . . . . 592 CN . . . . . . . . . . . . . . . . . . . . . . . . . . . . 574 Combi-HT . . . . . . . . . . . . . . . . . . . . . . 553 Eclipse AAA . . . . . . . . . . . . . . . . . . . . 598 Eclipse DsDNA. . . . . . . . . . . . . . . . . . 593 Eclipse XDB . . . . . . . . . . . . . . . . 539540 Extend-C18 300 . . . . . . . . . . . . . . . . 549 Extend-C18 80 . . . . . . . . . . . . . . . . . 546 GF-250. . . . . . . . . . . . . . . . . . . . . . . . . 585 GF-450. . . . . . . . . . . . . . . . . . . . . . . . . 585 HPLC Capillary . . . . . . . . . . . . . . . . . . 561 LC/MS . . . . . . . . . . . . . . . . . . . . 557559 Method Development Kits . . . . . . . . 596 NH2 . . . . . . . . . . . . . . . . . . . . . . . . . . . 574 Normal Phase . . . . . . . . . . . . . . . . . . 574 Oligo . . . . . . . . . . . . . . . . . . . . . . . . . . 594 Original RP C18 . . . . . . . . . . . . . . . . . 562 Original RP C8 . . . . . . . . . . . . . . . . . . 562 Original RP CN . . . . . . . . . . . . . . . . . . 562 Original RP Phenyl . . . . . . . . . . . . . . . 562 Original RP TMS . . . . . . . . . . . . . . . . 562 Poroshell 300SB-C18. . . . . . . . . . . . . 536 PSM . . . . . . . . . . . . . . . . . . . . . . . . . . 581 Rapid Resolution . . . . . . . . . . . . 557561 Rx . . . . . . . . . . . . . . . . . . . . . . . . . . . . 551 Rx-SIL . . . . . . . . . . . . . . . . . . . . . . . . . 574 SAX . . . . . . . . . . . . . . . . . . . . . . . . . . . 590 SCX . . . . . . . . . . . . . . . . . . . . . . . . . . . 590 SIL . . . . . . . . . . . . . . . . . . . . . . . . . . . . 574 Stablebond 300 . . . . . . . . . . . . . . . 534 Stablebond 80 . . . . . . . . . . . . 528529 Stablebond Aq . . . . . . . . . . . . . . . . . . 531 LC Standards, Column Selectivity Evaluation . . . . . . . . . .95 GPC-SEC Calibration Standards . . . . . . . .579 LC/MS . . . . . . . . . . . . . . . . . . . . . . . . . . . . .94 Qualitative . . . . . . . . . . . . . . . . . . . . . .94, 482 LC/MS Standards, Calibration, Performance . . . . . . . . . . . . .483 Qualitative . . . . . . . . . . . . . . . . . . . . . .94, 482 LC/MS Supplies, Electrospray . . . . . . . . . . . . . . . . . . . . . . . .484 Maintenance . . . . . . . . . . . . . . . . . . . . . . .483 Particle Beam . . . . . . . . . . . . . . . . . . . . . .484 Thermospray . . . . . . . . . . . . . . . . . . . . . . .484 Leak Detector, Gas . . . . . . . . . . . . . . . . . .75, 80 Lens Cleaning Paper . . . . . . . . . . . . . . . . . . .694 LiChrosorb LC Columns . . . . . . . . . . . . . . . . .569 LiChrospher Columns, CN . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .568 Normal Phase . . . . . . . . . . . . . . . . . . . . . .575 RP-18 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .568 RP-18 Endcapped . . . . . . . . . . . . . . . . . . .568 RP-8 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .568 RP-Select B . . . . . . . . . . . . . . . . . . . . . . . .568 Liners, Direct . . . . . . . . . . . . . . . . . . . . . . . . . . . . .157 Direct Connect . . . . . . . . . . . . . . . . . . . . . .158 Focus . . . . . . . . . . . . . . . . . . . . . . . . . . . . .158 Liner Ferrules . . . . . . . . . . . . . . . . . . . . . .159 Liner O-Rings . . . . . . . . . . . . . . . . . . . . . . .158 Megabore . . . . . . . . . . . . . . . . . . . . . . . . . .159 Megaliner . . . . . . . . . . . . . . . . . . . . . . . . . .158 Split/Splitless . . . . . . . . . . . . . . . . . . . . . .157 Liquid Sampling Valves . . . . . . . . . . . . . . . . .167
M
Magnifier . . . . . . . . . . . . . . . . . . . . . . . . .89, 314 Mass Flow Controllers, 5890/6890 . . . . . . .198 MEKC Buffer for Neutral and Charged Analytes . . . . . . . . . . . . . . . . . . .654 Merlin Microseal . . . . . . . . . . . . . . . . . . . . .154 Microprobe Set . . . . . . . . . . . . . . . . . . . . . . . .89 Micro-V Vials . . . . . . . . . . . . . . . . . . . .16, 21, 25 Microvolume Inserts, For Standard Opening (8-425) Vials . . . . . .31 For Wide Opening Vials . . . . . . . . . . . . . . .28 Mirror, Inspection . . . . . . . . . . . . . . . . . . . . . .89 Mounting Panels & Clips for Gas Traps . . . . .70 MS Engine, Cleaning and Maintenance Supplies . . . . . . . . . . . .202, 205, 214215 Electron Multiplier . . . . . . . . . . . . . . . . . .215 Ion Source Parts and Supplies . . . . .214215 Pump Lubricants and Oils . . . . . . . . . . . . .215 MS Interface Column Nut . . . . . . . . . . . . . . .201 MS Test and Performance Samples . . . .98, 216 MSD, Calibration/Tuning Standards . . . . . . . . .216 Cleaning and Maintenance Supplies . .202206 Electron Multipliers and Replacement Horn . . . . . . . . . . . . . . . .210 Filaments . . . . . . . . . . . . . . . . . . . . . . . . . .207 Ion Source Parts (EI), 5973 . . . . . . . . . . . . . . . . . . . . . . . . . . 206 5972/5971/GCD MSD. . . . . . . . . . . . 207 Maintenance Kit (For Diffusion Pump Systems) . . . . . . . . . . . . . . . . . . . . . . . .206 MSD NoVent . . . . . . . . . . . . . . . . . . . . . . .212 MSD NoVent . . . . . . . . . . . . . . . . . . . . . . . . .212 MSD, 1100 LC, Cleaning and Maintenance Supplies . . . .483 Electrospray Interface . . . . . . . . . . . . . . . .484 Particle Beam . . . . . . . . . . . . . . . . . . . . . .484 Thermospray . . . . . . . . . . . . . . . . . . . . . . .484 Multicell Transport Supplies, UV-Vis . . . .694, 696
J
Jets, FID, 5890 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .193 6890/6850 . . . . . . . . . . . . . . . . . . . . .182183 Jets, NPD, 5890 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .193 6890 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .184
K L
Labels, Bar Code Reader . . . . . . . . . . . . . . .173 Lamps, LC Detectors . . . . . . . . . . . . . . . . . . . . . . .455 UV-Vis . . . . . . . . . . . . . . . . . . . . . . . . . . . . .697 LC Columns, Agilent Cartridge System . . . . . . . . . . . . .597 Amino Acid Separations . . . . . . . . . . . . . .598 Asahipak LC Columns . . . . . . . . . . . . . . . .570 BioScience, SynChropak, Vydac . . . . . . . . . . . . . . 572 Cartridge Hardware . . . . . . . . . . . . . .512517 ChiraDex . . . . . . . . . . . . . . . . . . . . . . . . . . .577 Chiral, ChiraDex . . . . . . . . . . . . . . . . . . . . . . . 577 Ultron . . . . . . . . . . . . . . . . . . . . . . . . . 576 Column ID Module . . . . . . . . . . . . . . . . . .565 GPC-SEC, PLgel . . . . . . . . . . . . . . . . . . . . . . . . . . 584 Start-up Kits . . . . . . . . . . . . . . . . . . . . 578 ZORBAX PSM . . . . . . . . . . . . . . . . . . 581 Hypersil, BDS-C18 . . . . . . . . . . . . . . . . . . . . . . . 567 Guards . . . . . . . . . . . . . . . . . . . . . . . . 567 HPLC Capillary . . . . . . . . . . . . . . . . . . 567 MOS . . . . . . . . . . . . . . . . . . . . . . . . . . 567 Normal Phase . . . . . . . . . . . . . . . . . . 575 ODS . . . . . . . . . . . . . . . . . . . . . . . . . . . 567
Indices
704
Alphabetical Index
N
Nebulizer, CE/MS . . . . . . . . . . . . . . . . . . . . .657 Nebulizers & Supplies, ICP-MS, 4500 . . . . . . . . . . . . . . . . . . . . . . . . . .682683 7500 . . . . . . . . . . . . . . . . . . . . . . . . . .673674 NPD Beads, 5890 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .193 6890 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .184 NPD Supplies, 5890 . . . . . . . . . . . . . . . . . . . . . . . . . .192193 6890 . . . . . . . . . . . . . . . . . . . . . . . . . .184185 Nucleosil LC Columns . . . . . . . . . . . . . . . . . .569 Nuts (Fittings) . . . . . . . . . . . . . . . . . . . . . . . . .83 Nuts and Ferrules Set . . . . . . . . . . . . . . . . . . .83 Regulators, 5890/6890 . . . . . . . . . . . . . . . . .198 Retention Gaps . . . . . . . . . . . . . . . . . . . . . . .311 Rheodyne Valves . . . . . . . . . . . . . . . . . . . . . .450 Rinse Kit, GC Columns . . . . . . . . . . . . . .88, 314 Rotor Seals, LC . . . . . . . . . . . . . . . . . . . .451, 496 Rotors, GC Valve . . . . . . . . . . . . . . . . . . . . . .168 Snoop . . . . . . . . . . . . . . . . . . . . . . . . . . . . .79, 90 Software, Retention Time Locking . . . . . . . . . . . . . .108 Training . . . . . . . . . . . . . . . . . . . . . . . . . . . .107 Solvent Filters, Filter/Degasser . . . . . . . . . . . . . . . . . . . . .449 In-line . . . . . . . . . . . . . . . . . . . . . . . . . . . . .448 Solvent Reservoir, 1100 . . . . . . . . . . . . . . . . . . . . . . . . . .464465 1050/1090 . . . . . . . . . . . . . . . . . . . . .485, 492 SPE Products, AccuBOND II, Bulk SPE Sorbents. . . . . . . . . . . . . . . 129 Cartridge Starter Kits. . . . . . . . . . . . . 129 Empty Reservoirs & Frits. . . . . . . . . . 138 Ion Exchange . . . . . . . . . . . . . . . 124125 Non-silica . . . . . . . . . . . . . . . . . . 127128 Normal Phase (Non-polar) . . . . . . . . 122 Normal Phase (Polar) . . . . . . . . 123124 Reversed Phase . . . . . . . . . . . . . 120122 Evidex II . . . . . . . . . . . . . . . . . . . . . . . . . . .130 Parts and Disposables, 20-Port . . . . . . . . . . . . . . . . . . . . . . . . 134 96-Well Plate . . . . . . . . . . . . . . . . . . . 136 Individual Cartridge . . . . . . . . . . . . . . 134 J&W Manifold . . . . . . . . . . . . . . . . . . 137 Vacuum Manifolds, 10-Port . . . . . . . . . . . . . . . . . . . . . . . . 133 20-Port . . . . . . . . . . . . . . . . . . . . . . . . 134 96-Well Plate . . . . . . . . . . . . . . . . . . . 135 Individual Cartridge . . . . . . . . . . 134, 137 J&W Manifold . . . . . . . . . . . . . . . . . . 137 Zorbax Sorbents, C18 . . . . . . . . . . . . . . . . . . . . . . . . . . . 114 C18 EC. . . . . . . . . . . . . . . . . . . . . . . . . 116 C8 . . . . . . . . . . . . . . . . . . . . . . . . . . . . 118 Silica . . . . . . . . . . . . . . . . . . . . . . . . . . 119 Special Applications LC Columns . . . .598599 Split Vent Trap, 5890 Internal & External . . . .70 Split/Splitless Inlet Supplies, 5890 . . . . . . . . . . . . . . . . . . . . . . . . . .188189 6890/6850 . . . . . . . . . . . . . . . . . . . . .175176 Splitter, GC Column, Fixed & Variable . . . . . . . . . . . . . . . . . . . . .166 Glass, Press-fit . . . . . . . . . . . . . . . . . . . . .165 Spray Chambers, ICP-MS, 4500 . . . . . . . . . . . . . . . . . . . . . . . . . .683684 7500 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .674 Stainless Steel Tubing, GC, Deactivated . . . . . . . . . . . . . . . . . . . . . . . .312 Undeactivated . . . . . . . . . . . . . . . . . . . . . .313 Standard Reference Material (SRM) 869 . . . .95 Standards, Boiling Point Calibration, Qualitative . . . . .99 CE OQ/PV and IQ . . . . . . . . . . . . . . . . . . . .93 Gas Analyzer . . . . . . . . . . . . . . . . . . .100101 GC, Column Test . . . . . . . . . . . . . . . . . . 9697 GC/MS Evaluation. . . . . . . . . . . . 98, 216 Nickel Catalyst . . . . . . . . . . . . . . . . . . 100 Valve Checkout . . . . . . . . . . . . . . . . . 100 GFC Calibration . . . . . . . . . . . . . . . . . . . . . .92 GPC Calibration . . . . . . . . . . . . . . . . . . . . . .92 GPC-SEC Calibration . . . . . . . . . . . . . . . . .579 ICP-MS, Calibration . . . . . . . . . . . . . . . . . . . . . 689 Checkout Solutions . . . . . . . . . . . . . . 689 Environmental . . . . . . . . . . . . . . . . . . 690 Interference Check Mixes. . . . . . . . . 690 Tuning Solutions . . . . . . . . . . . . . . . . 689 LC, Column Selectivity Evaluation . . . . . . 95
S
Sample and Storage Vials . . . . . . . . . . . . .3536 Sample Cleanup Columns, LC . . . . . . . . . . . .518 Sample Loops, GC . . . . . . . . . . . . . . . . . . . . .168 Sample Loops, LC, PEEK . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .453 Stainless Steel . . . . . . . . . . . . . . . . . .452, 487 Titanium . . . . . . . . . . . . . . . . . . . . . . . . . . .453 Sample Preparation, CombiZorb Scavengers . . . . . . . . . . . . . . .141 Disposable Syringes . . . . . . . . . . . . . . . . .147 Syringe Filters . . . . . . . . . . . . . . . . . .146147 Sampling Cones, ICP-MS, 4500 . . . . . . . . . . . . . . . . . . . . . . . . . .685686 7500 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .676 7500c . . . . . . . . . . . . . . . . . . . . . . . . . . . . .676 SantoVac Ultra 5P . . . . . . . . . . . . . . . . . . . . .209 Scavengers, Combizorb . . . . . . . . . . . . . . . . .141 Screw Caps, 4 mL Vial . . . . . . . . . . . . . . . . . . . . . . . . . . . .32 Septa, For Standard Opening (8-425) . . . . . .30 For Wide Opening . . . . . . . . . . . . . . . . 17 Standard Opening (8-425) . . . . . . . . . . . . . .30 Wide Opening with Integrated Septa . . . .17 Wide Opening . . . . . . . . . . . . . . . . . . . .1617 Screw Top Vials, 4 mL . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .32 Standard Opening (8-425) . . . . . . . . . . . . . .29 Wide Opening, Glass . . . . . . . . . . . . . . . . . . . . . . . . . . . 15 High Recovery, Micro-V Vial . . . . . . . . 16 Kits and Convenience Packs . . . . 1819 Screwdrivers . . . . . . . . . . . . . . . . . . . . . . . . . .87 Semi-Volatiles (EPA 8270) Application Kit . .212 Septa, GC Inlet, Advanced Green . . . . . . . . . . . . . . . . . . . .152 Blue . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .153 Bleed and Temperature Optimized . . . . . .151 General Purpose . . . . . . . . . . . . . . . . . . . .153 Gray, Low-bleed . . . . . . . . . . . . . . . . . . . . .153 Long-Life . . . . . . . . . . . . . . . . . . . . . . . . . .151 Merlin Microseal . . . . . . . . . . . . . . . . . . .154 Red, Low-bleed . . . . . . . . . . . . . . . . . . . . .153 White (For 5700, 5830/40) . . . . . . . . . . . .153 Septa, Vial, GC Autosampler Wash Vial . . . . . . . . . . . .32 Headspace . . . . . . . . . . . . . . . . . . . . . . . . .35 Standard Opening (8-425) Screw Top . . . .30 Wide Opening Screw Top . . . . . . . . . . . . . .17 SimDis Standards . . . . . . . . . . . . . . . . . . . . . .99 Sipper Supplies, UV-Vis . . . . . . . . . . . . . . . . .697 Skimmer Cones, ICP-MS, 4500 . . . . . . . . . . . . . . . . . . . . . . . . . .685686 7500 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .676 7500c . . . . . . . . . . . . . . . . . . . . . . . . . . . . .676 Snap Caps, Wide Opening . . . . . . . . . . . . . . .26 Snap Top Vials, Kits and Convenience Packs . . . . . . . . . . .27 Wide Opening, Glass. . . . . . . . . . . . . . . . . . . . . . . . 2425 High Recovery, Micro-V Vial . . . . . . . . 25 Polypropylene. . . . . . . . . . . . . . . . . 22, 25
O
Oil, Vacuum Pump . . . . . . . . . . . . . . . . . . . . .209 Optical Filter, CE . . . . . . . . . . . . . . . . . . .656, 659 O-rings, GC Inlet Liner . . . . . . . . . . . . . . . . . .158
P
Packed Inlet Supplies, 5890 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .191 6890/6850 . . . . . . . . . . . . . . . . . . . . . . . . .180 Peltier Supplies, UV-Vis . . . . . . . . . . . . . . . . .697 Peristaltic Pump Tubing, ICP-MS, 4500 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .680 7500 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .670 Petrochemical Standards . . . . . . . . . . . . . . . .99 PFDTD Sample . . . . . . . . . . . . . . . . . . .208, 216 PFTBA Sample . . . . . . . . . . . . . . . . . . . .208, 216 Piston Seals, LC Pumps & Autosamplers . .455, 463 PL Aquagel-OH LC Columns . . . . . . . . . . . . .588 Pliers and Tweezers . . . . . . . . . . . . . . . . . . . . .87 Polyimide Sealing Resin . . . . . . . . . . . . . . . .164 Press-Fit Connectors . . . . . . . . . . . . . . . . . . .164 PTV Inlet Supplies, 6890 . . . . . . . . . . . . . . . .177 Pump Oil, Vacuum . . . . . . . . . . . . . . . . . . . . .209 Pump Pistons, LC . . . . . . . . . . . . .455, 463, 465 Pump Seals, LC . . . . . . . . . . . . . . . . . . .455, 463 Pump Supplies, LC, 1100, Active Inlet Valve & Supplies . . . . . . 463 Capillary Pump . . . . . . . . . . . . . . . . . . 464 Isocratic, Binary, Quat . . . . . . . . . . . . 463 Outlet Ball Valve & Supplies. . . 462463 Prep Pump . . . . . . . . . . . . . . . . . . . . . 465 Purge Valve & Supplies. . . . . . . 462463 Seal Wash & Supplies . . . . . . . . . . . . 462 1050 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .485 1090 Solvent Delivery . . . . . . . . . . . .492494 1090 Solvent Mixer . . . . . . . . . . . . . . . . . .494 Pistons . . . . . . . . . . . . . . . . . . . .455, 463, 465 Solvent Bottles & Supplies . . . .464, 485, 492 Solvent Inlet Filters . . . . . . . . . . . . . . . . . .464 Solvent Mixer, Static . . . . . . . . . . . . . . . . .494 Purge & Trap Supplies . . . . . . . . . . . . . .170172 Purospher LC Columns . . . . . . . . . . . . . . . . .570
Q R
Racks, Vial . . . . . . . . . . . . . . . . . . . . . . . . . . . .38 Reducing Ferrules . . . . . . . . . . . . . . . . . . . . .163
Indices
705
Alphabetical Index
LC, Column Selectivity Evaluation continued, LC/MS . . . . . . . . . . . . . . . . . 94, 482483 Qualitative . . . . . . . . . . . . . . . . . . . . . . 94 MIDI Calibration . . . . . . . . . . . . . . . . . . . .100 Petrochemical, Qualitative . . . . . . . . . . . . .99 UV-Vis OQ/PV and IQ . . . . . . . . . . . . .94, 699 Stirring Bar, UV-Vis . . . . . . . . . . . . . . . .694, 696 Straight Ferrules . . . . . . . . . . . . . . . . . . . . . .163 Superspher LC Columns . . . . . . . . . . . . . . . .571 Swabs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .91 SynChropak Columns, Hydrophobic Interaction . . . . . . . . . . . . . .591 Ion Exchange . . . . . . . . . . . . . . . . . . . . . . .591 Wide-Pore . . . . . . . . . . . . . . . . . . . . . . . . .572 Syringe Needles, Fused Silica . . . . . . . . . . . .45 Syringes, Disposable, 20 mL . . . . . . . . . . . . . . . . . . .147 GC Autosampler, 7670/71/72 . . . . . . . . . . . . . . . . . . . . . 42 7673/7683 . . . . . . . . . . . . . . . . . . . 4041 On-column . . . . . . . . . . . . . . . . . . . . . . 42 Manual, For LC Injector Valves . . . . . . . . . 46, 454 Gas-Tight Luer Lock. . . . . . . . . . . . . . . 46 Gas-Tight Plunger . . . . . . . . . . . . . . . . 44 Gas-Tight with Luer Lock Valve (1-50 mL) . . . . . . . . . . . . . . . . 45 Low Volume (0.5-5.0 L) . . . . . . . . . . . 43 On-column . . . . . . . . . . . . . . . . . . . . . . 45 Regular Plunger . . . . . . . . . . . . . . . . . . 43 SuperfleX Plunger . . . . . . . . . . . . . . . . 44 7500 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .675 Training Software . . . . . . . . . . . . . . . . . . . . . .107 Traps, A Selection Guide . . . . . . . . . . . . . . . . .4849 Combination Traps, Big Universal (RMSH Series) . . . . . . . 62 Gas Specific for MS. . . . . . . . . . . . 6162 Hydocarbon/Moisture Removal. . . . . 61 Oxygen/Moisture Removal . . . . . 6061 Gas Purification Systems, High Capacity. . . . . . . . . . . . . . . . . 6364 Point of Operation Panels. . . . . . . 6567 Super Clean . . . . . . . . . . . . . . . . . . 6869 GC Instrument, Detector, 5890 . . . . . . . . . . . . . . . . . . . 70 Internal Split Vent, 5890 . . . . . . . . . . . 70 Split Vent Trap . . . . . . . . . . . . . . . . . . . 70 Hydrocarbon Removal . . . . . . . . . . . . . .5758 Moisture Removal . . . . . . . . . . . . . . . .5155 Oxygen Removal . . . . . . . . . . . . . . . . . .5657 Trap Mounting Panel/Clips . . . . . . . . . . . .70 Traps, Particle . . . . . . . . . . . . . . . . . . . . . . . . .291 Tubing, Fused Silica, Deactivated . . . . . . . . . . . . . . . . . . . . 311 High-temperature. . . . . . . . . . . . . . . . 312 Undeactivated . . . . . . . . . . . . . . . . . . 313 Gas Supply, Copper. . . . . . . . . . . . . . . . . . . . . . . . . . 82 Stainless Steel (Spectra-Link) . . . . . . 82 ICP-MS, 4500 . . . . . . . . . . . . . . . . . . . . . . . . . . 680 7500 . . . . . . . . . . . . . . . . . . . . . . 669670 LC, 1050 Module Connectors . . . . . . . . . 486 1100 Module Connectors . . . 445, 459, 486 Capillary LC, Fused Silica/PEEK. . . 472473 Color Code Labels . . . . . . . . . . . . . . . 447 Kits . . . . . . . . . . . . . . . . . . . . . . . . . . . 446 PEEK . . . . . . . . . . . . . . . . . . . . . . . . . . 446 PEEK Tubing Cutter . . . . . . . . . . . . . . 447 Stainless Steel, Flexible. . . . 445446, 459 Stainless Steel, Rigid. . . . . . . . . . . . . 445 Stainless Steel (ProSteel), Deactivated . . . . . . . . . . . . . . . . . . . . 312 Undeactivated . . . . . . . . . . . . . . . . . . 313 UV-Vis . . . . . . . . . . . . . . . . . . . . . . . . . . . . .695 Tubing Cutters . . . . . . . . . . . . . . . . . .8788, 314 Tubing, LC, 1050 Module Connectors . . . . . . . . . . . . .486 1100 Module Connectors . . . . .445, 459, 486 Capillary LC, Fused Silica/PEEK . . . . .472473 Color Code Labels . . . . . . . . . . . . . . . . . . .447 Kits . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .446 PEEK . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .446 PEEK Tubing Cutter . . . . . . . . . . . . . . . . . .447 Stainless Steel, Flexible . . . . . .445446, 459 Stainless Steel, Rigid . . . . . . . . . . . . . . . .445 Tuning Samples, GC/MS . . . . . . . .98, 208, 216 Macro (Standard). . . . . . . . . . . . . . . . 692 Semi-micro . . . . . . . . . . . . . . . . . . . . . 693 Ultra-micro . . . . . . . . . . . . . . . . . . . . . 693 Dissolution Testing Supplies . . . . . . . . . .698 Lamps . . . . . . . . . . . . . . . . . . . . . . . . . . . . .697 Multicell Transport Supplies . . . . . . . . . . .696 Peltier Supplies . . . . . . . . . . . . . . . . . . . . .697 Sipper Supplies . . . . . . . . . . . . . . . . . . . . .697 Standards, Checkout Samples. . . . . . . . . . . . . . . 699 Holmium Oxide. . . . . . . . . . . . . . . . . . . 93 Hoya 056 Filter . . . . . . . . . . . . . . . . . . . 94 OQ/PV and IQ Kits. . . . . . . . . . . . 94, 699 Qualitative . . . . . . . . . . . . . . . . . . . 9394 Tubing & Fittings . . . . . . . . . . . . . . . .695, 698
V
Vacuum Degasser Supplies, 1050 Degasser . . . . . . . . . . . . . . . . . .460, 486 1100 Degassers . . . . . . . . . . . . . . . . .460, 486 Vacuum Manifolds, SPE, 10-Port . . . . . . . . . . . . . . . . . . . . . . . . . . . .133 20-Port . . . . . . . . . . . . . . . . . . . . . . . . . . . .134 96-Well Plate . . . . . . . . . . . . . . . . . . . . . . .135 Individual Cartridge . . . . . . . . . . . . . .134, 137 Vacuum Pump Oil . . . . . . . . . . . . . . . . . . . . .209 Vacuum Pump Supplies, ICP-MS, 4500 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .687 7500 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .678 Vacuum System & Pump Supplies, MS . . . .209 Valve Supplies, LC, 1050 Manual Injector . . . . . . . .450451, 486 1050 Manual Injector, PEEK . . . . . . . . . . .487 1090 Manual Injector . . . . . . . . . . . . . . . .496 1100 Manual Injector . . . . . . . . . . . . . . . .450 Column Switching . . . . . . . . . . . . . . .496497 Miscellaneous . . . . . . . . . . . . . . . . . . . . . .487 Rotor Seals . . . . . . . . . . . . . . . .451, 487, 496 Sample Loops, PEEK . . . . . . . . . . . . . . . . . . . . . . 453, 487 Stainless Steel . . . . . . . . . . . . . . 452, 487 Titanium . . . . . . . . . . . . . . . . . . . . . . . 453 Stator Face . . . . . . . . . . . . . . . . . . . . .451, 487 Stators . . . . . . . . . . . . . . . . . . . . . . . .451, 487 Valves & Loops, GC, Gas Sampling Valves . . . . . . . . . . . . . . . .167 Liquid Sampling Valves . . . . . . . . . . . . . . .167 Sample Loops . . . . . . . . . . . . . . . . . . . . . .168 Valve Rotors . . . . . . . . . . . . . . . . . . . . . . . .168 Valve Supplies . . . . . . . . . . . . . . . . . .167168 Vial Inserts, For Standard Opening (8-425) Vials . . . . . .31 For Wide Opening Vials . . . . . . . . . . . . . . .28 Vial Racks . . . . . . . . . . . . . . . . . . . . . . . . . . . . .38 Vials, GC Autosampler Wash Vial . . . . . . . . . . . .32 Headspace . . . . . . . . . . . . . . . . . . . . . . . . . .33 ICP-MS . . . . . . . . . . . . . . . . . . . . . . . .671, 680 Sample and Storage . . . . . . . . . . . . . . .3536 Wide Opening . . . . . . . . . . . . . . . . . . . .1426 Vials, Crimp Top, Kits and Convenience Packs . . . . . . . . . . .23 Wide Opening, Extreme Recovery . . . . . . . . . . . . . . . . 22 Glass . . . . . . . . . . . . . . . . . . . . . . . . . . . 20 Glass, Deactivated. . . . . . . . . . . . . . . . 21 Glass-lined Plastic . . . . . . . . . . . . 22, 26 High Recovery, Micro-V Vial . . . . . . . . 21 Polypropylene. . . . . . . . . . . . . . . . . 22, 25 Vials, Headspace . . . . . . . . . . . . . . . . . . . . . . .33 Vials, Screw Top, 4 mL . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .32 Kits and Convenience Packs . . . . . . . .1819
T
TCD Supplies, 5890 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .194 6890/6850 . . . . . . . . . . . . . . . . . . . . . . . . .185 Test Samples . . . . . . . . . . . . . . . . . . . . . . . .98 Tees (Fittings) . . . . . . . . . . . . . . . . . . . . . . . . . .84 Test Solutions, LC, 1100 MSD . . . . . . . . . . . . . . . . . . . . . . . . . .483 1100 MSD OQ/PV . . . . . . . . . . . . . . . . . . .482 1100 OQ/PV . . . . . . . . . . . . . . . . . . . . . . . .482 1100 Qualitative . . . . . . . . . . . . . . . . . . . . .482 LC/MS . . . . . . . . . . . . . . . . . . . . . . . . . . . . .94 Qualitative . . . . . . . . . . . . . . . . . . . . . . . . . .94 Tool Kit, General Laboratory . . . . . . . . . . . . . .86 Tools, 1100 Series LC . . . . . . . . . . . . . . . . . . . . . .458 Column Installation Kit, GC . . . . . . . .88, 314 Crimping & Decapping . . . . . . . . . . . . .3738 Ferrule Installation Tool . . . . . . . . . . .89, 314 Ferrule Tool Kit . . . . . . . . . . . . . . . . . . . . . . .91 FID Cleaning Kit . . . . . . . . . . . . . . . . . . . . . .90 File Set, 12-Piece . . . . . . . . . . . . . . . . . . . .90 Forceps . . . . . . . . . . . . . . . . . . . . . . . . . . . . .87 GC Buddy Multi-purpose Tool . . . . . . . . . .91 GC Column Cutting Tools . . . . . . . . . .88, 314 GC Column Ferrule Tools . . . . . . . . . . . . . .89 GC Column Rinse Kit . . . . . . . . . . . . . .88, 314 GC Inspection Tools . . . . . . . . . . . . . .89, 314 General Laboratory Tool Kit . . . . . . . . . . . .86 Hex Keys . . . . . . . . . . . . . . . . . . . . . . . . . . .87 Injection Port Cleaning Kit . . . . . . . . . . . . .90 Inlet Cleaning Wires . . . . . . . . . . . . . . . . . .91 Instrument . . . . . . . . . . . . . . . . . . . . . . . . . .91 Magnifier . . . . . . . . . . . . . . . . . . . . . . .89, 314 Pliers & Tweezers . . . . . . . . . . . . . . . . . . . .87 Screwdrivers . . . . . . . . . . . . . . . . . . . . . . . .87 Tubing Cutters . . . . . . . . . . . . . . . .8788, 314 Wrenches . . . . . . . . . . . . . . . . . . . . . . . . . . .86 Torches & Components, ICP-MS, 4500 . . . . . . . . . . . . . . . . . . . . . . . . . .684685
U
Ultron Chiral LC Columns . . . . . . . . . . . . . . .576 Union, Fused Silica . . . . . . . . . . . . . . . . . . . .164 Unions, Fittings . . . . . . . . . . . . . . . . . . . . . . . . . . . . .84 Reducing . . . . . . . . . . . . . . . . . . . . . . . . . . .85 UV-Vis, Autosampler Supplies . . . . . . . . . . . . . . . .697 Cell Accessories . . . . . . . . . . . . . . . .694, 696 Cell Holder Supplies . . . . . . . . . . . . .694, 696 Cells, Cylindrical . . . . . . . . . . . . . . . . . . . . . 693 Flow-through . . . . . . . . . . . . . . . 693694
Indices
706
Alphabetical Index
Standard Opening (8-425) . . . . . . . . . . . . . . . .29 Wide Opening, Glass . . . . . . . . . . . . . . . . . . . . . . . . . . . 15 Glass, Deactivated. . . . . . . . . . . . . . . . 15 High Recovery, Micro-V Vial . . . . . . . . 16 Vials, Snap Top, Kits and Convenience Packs . . . . . . . . . . .27 Wide Opening, Glass . . . . . . . . . . . . . . . . . . . . . . . . . . . 24 Glass, Deactivated. . . . . . . . . . . . . . . . 25 High Recovery, Micro-V Vial . . . . . . . . 25 Polypropylene. . . . . . . . . . . . . . . . . 22, 25 Vydac, PAH Columns . . . . . . . . . . . . . . . . . . . . . . .599 Wide-Pore . . . . . . . . . . . . . . . . . . . . . . . . .572
W
Wash Vials, GC Autosampler . . . . . . . . . . . . .32 Water, CE-Grade . . . . . . . . . . . . . . . . . . . . . .653 Window Etching Tool, CE . . . . . . . . . . . . . . .659 Wrenches . . . . . . . . . . . . . . . . . . . . . . . . . . . . .86
X Y Z
ZORBAX, Bonus-RP . . . . . . . . . . . . . . . . . . . . . . . . . .543 Carbohydrate Analysis . . . . . . . . . . .574, 592 CN . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .574 CombiHT . . . . . . . . . . . . . . . . . . . . . . . . . . .553 Eclipse AAA . . . . . . . . . . . . . . . . . . . . . . . .598 Eclipse DsDNA . . . . . . . . . . . . . . . . . . . . .593 Eclipse XDB . . . . . . . . . . . . . . . . . . . .539540 Extend-C18 300 . . . . . . . . . . . . . . . . . . .549 Extend-C18 80 . . . . . . . . . . . . . . . . . . . .546 GF-250 . . . . . . . . . . . . . . . . . . . . . . . . . . . .585 GF-450 . . . . . . . . . . . . . . . . . . . . . . . . . . . .585 HPLC Capillary . . . . . . . . . . . . . . . . . . . . . .561 LC/MS . . . . . . . . . . . . . . . . . . . . . . . .557559 Method Development Kits . . . . . . . . . . . .596 NH2 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .574 Normal Phase . . . . . . . . . . . . . . . . . . . . . .574 Oligo . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .594 Original ZORBAX, RP C18 . . . . . . . . . . . . . . . . . . . . . . . . 562 RP C8 . . . . . . . . . . . . . . . . . . . . . . . . . 562 RP CN . . . . . . . . . . . . . . . . . . . . . . . . . 562 RP Phenyl . . . . . . . . . . . . . . . . . . . . . . 562 RP TMS. . . . . . . . . . . . . . . . . . . . . . . . 562 Poroshell 300SB-C18 . . . . . . . . . . . . . . . .536 PSM . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .581 Rapid Resolution . . . . . . . . . . . . . . . .557561 Rx . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .551 Rx-SIL . . . . . . . . . . . . . . . . . . . . . . . . . . . . .574 SAX . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .590 SCX . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .590 SIL . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .574 SPE Products, C18 . . . . . . . . . . . . . . . . . . . . . . . . . . . 114 C18 EC. . . . . . . . . . . . . . . . . . . . . . . . . 116 C8 . . . . . . . . . . . . . . . . . . . . . . . . . . . . 118 Silica . . . . . . . . . . . . . . . . . . . . . . . . . . 119 Stablebond 300 . . . . . . . . . . . . . . . . . . . .534 Stablebond 80 . . . . . . . . . . . . . . . . .528529 Stablebond Aq . . . . . . . . . . . . . . . . . . . . . .531 Stablebond Wide-Pore . . . . . . . . . . . . . . .574
Indices
707
Compound Index
A
Acenaphthalene . . . . . . . . . . . . . . .368, 626, 627 Acenaphthene . . . . . . . . . . . . . . . .366, 368, 537 Acenaphthene-d10 . . . . . . . . . . . . . . . .366, 367 Acenaphthylene . . . . . . . . . .366368, 626, 627 Acetal (acetaldehyde diethyl acetal) . . . . . . . . .403 Acetaldehyde . . .379380, 388, 398, 422423, 630 Acetaldehyde-DNPH . . . . . . . . . . . . . . . . . . .622 2-Acetamidophenol . . . . . . . . . . . . . . . .604, 643 Acetaminophen (4-acetamidophenol) .415, 421, 604, 643 Acetanilide . . . . . . . . . . . . . . . . . . . . . . .604, 643 Acetic Acid . . . . . . . . . . .388, 390, 395, 397, 621 Acetic acid, ethyl ester . . . . . . . . . . . . . . . . .436 Acetic acid, methyl ester . . . . . . . . . . . . . . .436 Acetaminophen . . . . . . . . . . . . . . . . . . . . . . .606 Acetone . . . . .371372, 379382, 388, 390, 398, 407408, 414, 422424 Acetone-d6 . . . . . . . . . . . . . . . . . . . . . . . . . . .380 Acetone-DNPH . . . . . . . . . . . . . . . . . . . . . . .622 Acetonitrile . . . . . . . . . .372, 408409, 423424 Acetophenone . . . . . . . . . . . . . . . . . . . . . . . .632 Acetylacetone . . . . . . . . . . . . . . . . . . . . . . . .618 Acetylcedrene . . . . . . . . . . . . . . . . . . . . . . . .383 6-Acetylcodeine . . . . . . . . . . . . . . . . . . .420421 Acetylene . . . . . . . . . . . . . . . . . . . .426430, 433 N-acetylprocainamide . . . . . . . . . . . . . . . . . .546 Acetylsalicylic acid (aspirin) . . . . . . . . . .421, 647 Acifluorofen . . . . . . . . . . . . . . . . . . . . . . . . . .357 Acrolein . . . . . . . . . . . . . . . . . . . . .372, 398, 409 Acrolein-DNPH . . . . . . . . . . . . . . . . . . . . . . .622 Acrylamide . . . . . . . . . . . . . . . . . . . . . . . . . . .397 Acrylates . . . . . . . . . . . . . . . . . . . . . . . . . . . .410 Acrylonitrile . . . . . . . . . . . . . .371372, 409410 Adenine . . . . . . . . . . . . . . . . . . . . . . . . . . . . .652 Adenosine . . . . . . . . . . . . . . . . . . . . . . . . . . .607 Adenylate Kinase . . . . . . . . . . . . . . . . . . . . . .614 Adrin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .352 Aflatoxins . . . . . . . . . . . . . . . . . . . . . . . . . . . .631 Air . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .425, 431 Alachlor . . . . . . . . .350352, 355356, 358, 367 Alanine (Ala) . . . . . . . . . . . . . . . . . . . . . . . . . .604 Albumin, Bovine Serum . . . .586587, 614616 Albumin, Chicken . . . . . . . . . . . . . . . . .587, 616 Alcohol dehydrogenase (ADH) . . . . . . . . . . .616 Alcohols, Blood . . . . . . . . . . . . . . .394395, 422 Aldehydes . . . . . . . . . . . . . . . . . . . . . . . .398, 622 Alditol acetates . . . . . . . . . . . . . . . . . . . . . . .388 Aldol . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .630 Aldolase . . . . . . . . . . . . . . . . . . . . . . . . . . . . .614 Aldrin . . . . . . . . . . . . . . . . . . . . . . .349355, 367 Alfentanyl . . . . . . . . . . . . . . . . . . . . . . . . . . . .419 Alkyd Resin . . . . . . . . . . . . . . . . . . . . . . . . . .618 Allobarbital . . . . . . . . . . . . . . . . . . . . . . .415, 418 Allyl acrylate . . . . . . . . . . . . . . . . . . . . . . . . . .410 Allyl alcohol . . . . . . . . . . . . . . . . . . . . . . . . . .372 Allyl butyrate . . . . . . . . . . . . . . . . . . . . .381382 Allyl chloride . . . . . . . . . . . . . . . . . . . . .371373 Allyl ether . . . . . . . . . . . . . . . . . . . . . . . . . . . .403 Allyl ethyl ether . . . . . . . . . . . . . . . . . . . . . . .403 Allyldisulfide . . . . . . . . . . . . . . . . . . . . . . . . . .384 Allylmethyldisulfide . . . . . . . . . . . . . . . . . . . .384 Allylmethyltrisulfide . . . . . . . . . . . . . . . . . . . .384 Allylpropenyldisulfide . . . . . . . . . . . . . . . . . .384 Allylsulfide . . . . . . . . . . . . . . . . . . . . . . . . . . .384 Allyltrisulfide . . . . . . . . . . . . . . . . . . . . . . . . .384 Allymethylsulfide . . . . . . . . . . . . . . . . . . . . . .384 Alphenal . . . . . . . . . . . . . . . . . . . . . . . . . . . . .418 Alprazolam . . . . . . . . . . . . . . . . . . . . . . . . . . .419 Altrazine . . . . . . . . . . . . . . . . . . . . . . . . . . . . .596 Amaranth . . . . . . . . . . . . . . . . . . . . . . . .634, 638 Ametryn . . . . . . . . . . . . . . . . . . . . .356, 367, 630 Ametryne . . . . . . . . . . . . . . . . . . . . . . . .358359 Amines . . . . . . . . . . . . . . . . . . . . . . . . . .396, 622 Amino Acids . . . . . . . . . . . . . . . . . . . . . . . . . .604 2-Aminoazotoluene . . . . . . . . . . . . . . . . . . . .411 3-Amino-benzonitrile . . . . . . . . . . . . . . . . . . .622 2-Amino-4,6-dinitrotoluene . . . . . . . . . .623, 624 4-Amino-2,6-dinitrotoluene . . . . . . . . . .623, 624 Aminoethylethanolamine . . . . . . . . . . . . . . .397 N-(2-Aminoethyl) piperazine . . . . . . . . . . . . . .397 2-Aminonaphthalene . . . . . . . . . . . . . . . . . . .411 2-Amino-4-nitrotoluene . . . . . . . . . . . . . . . . .624 2-Amino-6-nitrotoluene . . . . . . . . . . . . . . . . .624 4-Amino-2-nitrotoluene . . . . . . . . . . . . . . . . .624 Amitriptyline . . . . . . . . .416, 537, 605606, 650 cis 10-OH- Amitriptyline . . . . . . . . . . . . . . . .650 trans 10-OH- Amitriptyline . . . . . . . . . . . . . .650 Amobarbital . . . . . . . . . . . . . . . . . . . . . .415, 418 Amphetamine . . . . . . . . . . . . . . . . . . . . . . . . .416 -Amylase . . . . . . . . . . . . . . . . . . . . . . .585, 616 Amyl acetate . . . . . . . . . . . . . . . . . . . . .388, 402 n-Amyl alcohol . . . . . . . . . . . . . . . . . . . . . . . .388 n-Amylamine . . . . . . . . . . . . . . . . . . . . .372, 396 Amyl butyrate . . . . . . . . . . . . . . . . . . . . . . . . .388 n-Amyl mercaptan . . . . . . . . . . . . . . . . . . . . .434 tert-Amyl mercaptan . . . . . . . . . . . . . . . . . . .434 tert-Amyl methyl ether . . . . . . . . .347, 372, 403 n-Amyl salicylate . . . . . . . . . . . . . . . . . .381383 Analgesics . . . . . . . . . . . . . . . . . . .604, 643, 644 5-Androstan-17-ol-3-one (Stanolone) . . . .422 5--Androsterone . . . . . . . . . . . . . . . . . . . . .421 Androsterone . . . . . . . . . . . . . . . . . . . . . . . . .421 Anesthetics . . . . . . . . . . . . . . . . . . . . . .417, 643 Anethole . . . . . . . . . . . . . . . . . . . . . . . . . . . . .633 Angiotensins . . . . . . . . . . . . .535, 548, 611612 Anilines . . . . . . . . . . . . . . . . .366, 397, 411, 622 p-Anisidine . . . . . . . . . . . . . . . . . . . . . . . . . . .622 Anorectics . . . . . . . . . . . . . . . . . . . . . . . . . . .604 Antazoline . . . . . . . . . . . . . . . . . . . . . . . . . . .417 Anthocyanins . . . . . . . . . . . . . . . . . . . . . . . . .631 Anthracene . . . . . . . . . . . . . .366368, 626627 Anthranilic acid . . . . . . . . . . . . . . . . . . . . . . .623 Antibiotics . . . . . . . . . . . . . . . . . . . . . . . . . . .643 Antibodies . . . . . . . . . . . . . . . . . . . . . . . . . . .605 Anticonvulsants . . . . . . . . . . . . . . . . . . . . . . .417 Antidepressants . . . . . . . . . . . . . . . . . .605606 Antiepileptics . . . . . . . . . . . . . . . . . . . . . . . . .418 Antifungals . . . . . . . . . . . . . . . . . . . . . . . . . . .644 Antihistamanes . . . . . . . . . . . . . . . . . . .417, 644 Antioxidants . . . . . . . . . . . . . . . . . . . . . .631632 Apomyoglobin . . . . . . . . . . . . . . . . . . . . . . . .612 Apple Drink . . . . . . . . . . . . . . . . . . . . . . . . . . .637 Apple Juice . . . . . . . . . . . . . . . . . . . . . .637, 639 Aprobarbital . . . . . . . . . . . . . . . . . . . . . .415, 418 Aprotinin . . . . . . . . . . . . . . . . . . . . . . . . . . . . .614 Arabinitol . . . . . . . . . . . . . . . . . . . . . . . . . . . .388 D-Arabinose . . . . . . . . . . . . . . . . . . . . . . . . . .389 Arachidic acid . . . . . . . . . . . . . . . . . . . . . . . .392 Arachidic acid methyl ester (C20:0) . . . . . . . .392 Arachidonic acid methyl ester (C20:4n6) . . . .392 Arginine (Arg) . . . . . . . . . . . . . . . . . . . . . . . . .604 Argon . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .425 Arochlors . . . . . . . . . . . . . . . . . . . . . . . .361363 Aromatics . . . . . . . . . . . . . . . .399400, 435, 632 Arsine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .414 Aspartic acid (Asp) . . . . . . . . . . . . . . . . .527, 604 Aspartame . . . . . . . . . . . . . . . . . . .527, 555, 632 Aspartic acid-phenylalanine dipeptide (Asp-phe) . . . . . . . . . . . . . . . . . . .527, 555, 632 Aspirin (acetosalicylic acid) . . . . . . .421, 604, 643 Aspon . . . . . . . . . . . . . . . . . . . . . . . . . . .356357 Atenolol . . . . . . . . . . . . . . . . . . . . . . . . . . . . .647 Atraton . . . . . . . . . . . . . . . . . . . . . .356, 358359 Atrazine . . . .351352, 356359, 367, 624625, 627630 Azide . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .616 Azinphos ethyl . . . . . . . . . . . . . . . . . . . .356357 Azinphos methyl . . . . . . . . . . . . . . . . . .356357 Azobenzene . . . . . . . . . . . . . . . . . . . . . . . . . .366 Azulene . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .368
B
Barbital . . . . . . . . . . . . . . . . . . . . . . . . . .418, 538 Barbiturates . . . . . . . . . . . . . . . . . . . . . . . . . .418 Behenic acid methyl ester (C22:0) . . . . . . . . .392 Benactyzine . . . . . . . . . . . . . . . . . . . . . . . . . .415 Benazldehyde . . . . . . . . . . . . . . . . . . . . . . . . .630 Bencoic acid . . . . . . . . . . . . . . . . . . . . . . . . . .640 Bensulfuron-methyl . . . . . . . . . . . . . . . . . . . .630 Bentazon . . . . . . . . . . . . . . . .357, 596, 624625 Benylamine . . . . . . . . . . . . . . . . . . . . . . . . . .396 Benzaldehyde . . . . . . . . . . . .381383, 398, 436 Benzaldehyde-DNPH . . . . . . . . . . . . . . . . . . .622 1,2-Benzanthracene . . . . . . . . . . . . . . . . . . .368 Benzene . . . .347348, 371373, 375, 378379, 399401, 406408, 423424, 428430, 435, 437439 Benzene-d6 . . . . . . . . . . . . . . . . . . . . . . . . . .439 Benzene ethanol . . . . . . . . . . . . . . . . . .381383 Benzidine . . . . . . . . . . . . . . . . . . . . . . . .366, 411 Benzo(a)anthracene . . .366, 367, 626627, 712 Benzocaine . . . . . . . . . . . . . . . . . . . . . . . . . . .417 Benzodiazepines . . . . . . . . . . . . . . . . . . . . . .419 Benzo(a)fluoranthene . . . . . . . . . . . . . . . . . .627 Benzo(b)fluoranthene . . . . . . . . . .366368, 626 Benzo(k)fluoranthene . . . . . .366368, 626627 2,3-Benzofluorene . . . . . . . . . . . . . . . . . . . . .368 Benzoic acid . . . . . . . . . . . . . . . . . .366, 632633 Benzonitrile . . . . . . . . . . . . . . . . . . . . . .397, 409 Benzo(g,h,i)perylene . . . . . . .366368, 626627 Benzophenone . . . . . . . . . . . . . . . . . . . .381382 Benzo(a)pyrene . . . . . . . . . . .366368, 626627 Benzo(e)pyrene . . . . . . . . . . . . . . . . . . .368, 626 5,6-Benzoquinoline . . . . . . . . . . . . . . . . . . . .368 Benzothiophene . . . . . . . . . . . . . . . . . . . . . . .434 Benzoylecgonine . . . . . . . . . . . . . . . . . . . . . .647 Benzphetamine . . . . . . . . . . . . . . . . . . .415416 Benzthiazuron . . . . . . . . . . . . . . . . . . . . . . . .625 Benzyl acetate . . . . . . . . . . . .381383, 386, 402 n-6-Benzyl adenine . . . . . . . . . . . . . . . . . . . .629 Benzyl alcohol . . . . . . . . . . . .366, 381, 382, 394 Benzyl amine . . . . . . . . . . . . . . . . . . . . . . . . .397 Benzyl benzoate . . . . . .368, 381383, 386, 388
Indices
708
Compound Index
Benzyl butyl phthalate . . . . . . . . . . . . . . . . . .367 Benzyl chloride . . . . . . . . . . . . . . .372, 373, 379 5-benzyl-3, 6-dioxo-2-piperazineacetic acid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .527, 555 Benzyl ether . . . . . . . . . . . . . . . . . . . . . . . . . .403 N-Benzyl methylamine . . . . . . . . . . . . . . . . .397 Benzyl salicylate . . . . . . . . . . . . . .381, 383, 386 trans--Bergamotene . . . . . . . . . . . . . . . . . .384 BHA . . . . . . . . . . . . . . . . . . . . . . . . . . . .632, 640 -BHC . . . . . . . . . . . . . . . . . . . . . .349355, 367 -BHC . . . . . . . . . . . . . . . . . . . . . . .349355, 367 -BHC . . . . . . . . . . . . . . . . . . . . . . .349355, 367 -BHC . . . . . . . . . . . . . . . . . . . . . . .349355, 367 BHT . . . . . . . . . . . . . . . . . . . . .387, 391, 632, 640 Bifenox . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .628 Biphenyl . . . . . . . . . . . . . . . . . . . . . . . . . . . . .368 2,2-Bipyridine . . . . . . . . . . . . . . . . . . . . . . . .618 -Bisabolene . . . . . . . . . . . . . . . . . . . . .382, 384 -Blockers . . . . . . . . . . . . . . . . . . . . . . . . . . .605 Blue #1 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .634 Bolstar . . . . . . . . . . . . . . . . . . . . . . . . . .356357 Borneol . . . . . . . . . . . . . . . . . . . . . .381383, 387 Bornyl acetate . . . . . . . . . . . . . . . . . . . . . . . .381 Bourbon . . . . . . . . . . . . . . . . . . . . . . . . . . . . .388 -Bourbonene . . . . . . . . . . . . . . . . . . . .385, 386 BPE (PB) . . . . . . . . . . . . . . . . . . . . . . . . . . . . .364 Bromacil . . . . . . . . . . . . . . . . .356, 358, 367, 628 Bromazepam . . . . . . . . . . . . . . . . . . . . . . . . .419 Bromoacetic acid . . . . . . . . . . . . . . . . . . . . . .370 Bromoacetone . . . . . . . . . . . . . . . . . . . . . . . .372 4-Bromoaniline . . . . . . . . . . . . . . .397, 411, 622 Bromobenzene . . . . . . .371373, 375, 399400 2-Bromobiphenyl . . . . . . . . . . . . . . . . . . . . . .350 Bromochloroacetic acid . . . . . . . . . . . . . . . .370 Bromochloroacetonitrile . . . . . . . . . . . .376377 Bromochlorodifluoromethane . . . . . . . . . . . .380 Bromochloromethane . . . . . . . . .371373, 375, 378380, 405 Bromodichloroacetic acid . . . . . . . . . . . . . . .370 Bromodichloromethane371373, 375377, 379, 405, 413 2-Bromo-4,6-dinitroaniline . . . . . . . . . . . . . .411 Bromoethane (ethyl bromide) . . . . . . . . .379, 405 Bromofluorobenzene . . . . . . . . . .371, 372, 375, 377, 378, 379, 380 Bromoform . . . . . . .371373, 375377, 379, 405 Bromomethane . . . . . . .371373, 375, 378379 1-Bromo-2-nitrobenzene . . . . . . . . . . . . . . . .350 Bromopheniramine . . . . . . . . . . . . . . . .415, 417 4-Bromophenyl-phenylether . . . . . . . . . . . . .366 3-Bromopyridine . . . . . . . . . . . . . . . . . . . . . .397 Bromotrifluoromethane . . . . . . . . . . . . . . . . .380 BSA (Bovine Serum Albumin) . . . . . . . . .586587, 615616 Buclizine . . . . . . . . . . . . . . . . . . . . . . . . . . . . .417 Bufotenine . . . . . . . . . . . . . . . . . . . . . . . . . . .420 Butabarbital . . . . . . . . . . . . . . . . . . . . . .415, 418 Butacaine . . . . . . . . . . . . . . . . . . . .417, 526, 643 Butachlor . . . . . . . . . . . . . . . . . . . .356, 358, 367 1,2-Butadiene . . . . . . . . . . . . . . . . . . . . .428429 1,3-Butadiene . . . . . . . . . . . .379, 426430, 433 Butalbital . . . . . . . . . . . . . . . . . . . . . . . . . . . .418 Butanal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .397 iso-Butane . . . . . . . . . . . . . . . . . . .425, 431, 433 n-Butane . . . . . . . . . . . .425431, 433, 437438 tert-Butane . . . . . . . . . . . . . . . . . . . . . . . . . . .431 Butanediol . . . . . . . . . . . . . . . . . . . . . . .403404 2,3-Butanedione (diacetyl) . . . . . . . . . .381382 1-Butanethiol . . . . . . . . . . . . . . . . . . . . .432, 434 1-Butanol (n-butyl alcohol) . . . . . . . . .372, 388, 394395, 408, 410, 423424 436 iso-Butanol . . . . . . . . . . . . . . . . . . . . . . . . . . .395 sec-Butanol . . .388, 394, 407408, 423424, 436 tert-Butanol . . . . . .372, 394, 408, 423424, 436 2-Butanone . . . . . . . . . .371372, 379, 380, 398 2-Butanone-DNPH . . . . . . . . . . . . . . . . . . . . .622 Butene . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .402 1-Butene . . . . . . . .402, 426429, 430431, 433 cis-2-Butene . . . . . . . . . . . . . . . . .426431, 433 trans-2-Butene . . . . . . . . . . . . . . . .426431, 433 3-Buten-1-ol . . . . . . . . . . . . . . . . . . . . . . . . . .394 2-Buten-1-ol (crotyl alcohol) . . . . . . . . . . . . . .394 Butethal . . . . . . . . . . . . . . . . . . . . . . . . . . . . .418 2-Butoxyethanol (butyl Cellosolve) . . . . . . . . .394 bis(2-n-Butoxyethyl) phthalate . . . . . . . . . . . . .368 Buturon . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .628 Butyl acetate . . . . . . . . . . . . . . . . .402, 408, 410 sec-Butyl acetate . . . . . . . . . . . . . . . . . . . . . .402 tert-Butyl acetate . . . . . . . . . . . . . . . . . . . . . .402 Butyl acrylate . . . . . . . . . . . . . . . . . . . . . . . . .410 n-Butylamine . . . . . . . . . . . . . . . . . . . . . . . . .396 Butylate . . . . . . . . . . . . . . . . . . . . . . . . .356, 367 Butylbenzene . . . . . . . . . . . . .372, 399400, 437 n-Butylbenzene . . . . .371, 373, 389, 401, 407, 435 sec-Butylbenzene . . . . . . . . . . . . .371373, 375, 399400, 407, 435, 437 tert-Butylbenzene . . . . . . . . . . . . .371373, 375, 399400, 407, 435 Butyl benzyl phthalate . . . . . . . . . . . . . .366, 368 Butyl caproate . . . . . . . . . . . . . . . . . . . . . . . .402 Butyl cellosolve . . . . . . . . . . . . . . . . . . . . . . .408 Butyl cellosolve acetate . . . . . . . . . . . . . . . .408 Butyl ether . . . . . . . . . . . . . . . . . . . . . . . . . . .403 Butyl ethyl ether . . . . . . . . . . . . . . . . . . . . . .403 tert-Butyl ethyl ether . . . . . . . . . . . . . . . . . . .403 Butyl heptanoate . . . . . . . . . . . . . . . . . . . . . .402 1-Butyl mercaptan . . . . . . . . . . . . . . . . . . . . .434 n-Butyl mercaptan . . . . . . . . . . . . . . . . . . . . .434 tert-Butyl mercaptan . . . . . . . . . . . . . . . . . . .434 sec-Butyl methacrylate . . . . . . . . . . . . . . . . .410 Butyl methyl ether . . . . . . . . . . . . . . . . . . . . .403 tert-Butyl methyl ether (MTBE) . . . . . . . . . . .379 Butylparaben . . . . . . . . . . . . . . . . . . . . .537, 638 Butylpentyltin . . . . . . . . . . . . . . . . . . . . . . . . .365 Butyl propionate . . . . . . . . . . . . . . . . . .402, 410 4-tert-Butyltoluene . . . . . . . . . . . . . . . .399400 Butyl valerate . . . . . . . . . . . . . . . . . . . . . . . . .402 1-Butyne (ethylacetylene) . . . . . . . . . . . .428429 2-Butyne (dimethylacetylene) . . . . . . . . .428429 Butyraldehyde . . . . . . . . . . . . . . . . . . . .398, 630 n-Butyraldehyde-DNPH . . . . . . . . . . . . . . . . .622 Butyric acid . . . . . . . . . . . . . . . . . .390, 395, 397 iso-Butyric acid . . . . . . . . . . . . . . . . . . . . . . .397 Butyric acid methyl ester (C4:0) . . . . . . . . . .392 Calmodulin . . . . . . . . . . . . . . . . . . . . . . . . . . .611 Camphene . . . . . . . . . . . . . . . . . . .381384, 387 Camphor . . . . . . . . . . . . . . . . . . . . .381382, 387 Capric acid methyl ester (C10:0) . . . . . . . . . .392 Capricaldehyde . . . . . . . . . . . . . . . . . . . . . . .630 Caproic acid (hexanoic acid) . . . . . . . . . .390, 395 Caproic acid methyl ester (C6:0) . . . . . . . . . .392 Caproicaldehyde . . . . . . . . . . . . . . . . . . . . . .630 Caprylic acid methyl ester (C8:0) . . . . . . . . . .392 Captafol . . . . . . . . . . . . . . . . . . . . . . . . . . . . .350 Captan . . . . . . . . . . . . . . . . . . . . . . . . . .350, 355 Caramel Syrup . . . . . . . . . . . . . . . . . . . . . . . .637 Carbamazepine . . . . . . . . . . . . . . . . . . . . . . .417 Carbazepine . . . . . . . . . . . . . . . . . . . . . . . . . .416 Carbazole . . . . . . . . . . . . . . . . . . . .366, 368, 623 Carbendazim . . . . . . . . . . . . . . . . . . . . . . . . . .640 Carbepoxide 10/11 . . . . . . . . . . . . . . . . . . . .418 Carbetamide . . . . . . . . . . . . . . . . . . . . . . . . . .628 Carbinoxamine . . . . . . . . . . . . . . . . . . . . . . . .417 Carbohydrates . . . . . . . . . . . .592, 633, 635637 Carbon dioxide . . . . . . . . . . .378, 414416, 421, 425, 431, 647 Carbon disulfide . . . . . .371, 379, 380, 432434 Carbon monoxide . . . . . . . . . . . . . . . . . .425, 431 Carbon oxysulfide . . . . . . . . . . . . . .414, 431,434 Carbon tetrachloride . . . . . . . . . . . . . . .371373, 375379, 405, 423 Carbonic Anhydrase . . . . . . .533, 535, 611612 Carbonyl sulfide . . . . . . . . . . . . . . .380, 432433 Carbophenothion . . . . . . . . . . . . . . . . . .355357 Carboxin . . . . . . . . . . . . . . . . . . . . .356, 358, 367 Carbutilat . . . . . . . . . . . . . . . . . . . . . . . . . . . .628 Carfentanyl . . . . . . . . . . . . . . . . . . . . . . . . . . .419 -Carotene . . . . . . . . . . . . . . . . . . . . . . . . . . .641 cis-Carveol . . . . . . . . . . . . . . . . . . . . . . .383, 386 trans-Carveol . . . . . . . . . . . . . . . . . . . . .383, 386 Carvocrol . . . . . . . . . . . . . . . . . . . . . . . . . . . .383 Carvone . . . . . . . . . . . . . . . . . . . . . . . . . .386, 633 Carvonel . . . . . . . . . . . . . . . . . . . . . . . . . . . . .381 cis-Carvylacetate . . . . . . . . . . . . . . . . . . . . . .386 -Caryophyllene . . . . . . . . . .381382, 384386 Catechol . . . . . . . . . . . . . . . . . . . . . . . . .623, 635 Cateholamines . . . . . . . . . . . . . . . . . . . . . . . .645 CDR . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .533 Cefachlor . . . . . . . . . . . . . . . . . . . . . . . . . . . .606 Cefalexin . . . . . . . . . . . . . . . . . . . . . . . . . . . . .606 Cefamandole nafate . . . . . . . . . . . . . . . . . . .606 Cefatoxime . . . . . . . . . . . . . . . . . . . . . . . . . . .606 Cefazolin . . . . . . . . . . . . . . . . . . . . . . . . .606, 643 Cefotaxime . . . . . . . . . . . . . . . . . . . . . . . . . . .643 Cefoxitin . . . . . . . . . . . . . . . . . . . . . . . . . . . . .606 Ceftazidime . . . . . . . . . . . . . . . . . . . . . . . . . . .643 Celestocide . . . . . . . . . . . . . . . . . . . . . . . . . . .381 Cellosolve acetate . . . . . . . . . . . . . . . . . . . . .408 Cephaclor . . . . . . . . . . . . . . . . . . . . . . . . . . . .543 Cephalexin . . . . . . . . . . . . . . . . . . . . . . . . . . .543 Cephalosporins . . . . . . . . . . . . . . . . . . .543, 606 Cephalothin . . . . . . . . . . . . . . . . . . . . . . . . . .606 Cephoxitin . . . . . . . . . . . . . . . . . . . . . . . . . . .543 Cephuroxime . . . . . . . . . . . . . . . . . . . . . . . . .543 Cetearyl decanoate . . . . . . . . . . . . . . . .381382 Cetearyl octanoate . . . . . . . . . . . . . . . .381382 Chelating agents . . . . . . . . . . . . . . . . . . . . . .618 Chloral hydrate . . . . . . . . . . . . . . . . . . .376377 Chloramben . . . . . . . . . . . . . . . . . . . . . . . . . .357
C
Cadinene . . . . . . . . . . . . . . . . . . . . . . . . . . . . .385 d-Cadinene . . . . . . . . . . . . . . . . . . . . . . .381382 Caffeine . . . . . . . . . . . . .415416, 421, 538, 545, 556, 604, 643 Cafoperazone . . . . . . . . . . . . . . . . . . . . . . . . .606
Indices
709
Compound Index
Chlorbromuron . . . . . . . . . . . . . . . . . . . . . . . .628 Chlorcyclizine . . . . . . . . . . . . . . . . . . . . .415, 417 Chlordane . . . . . . . . . . . . . . . . . . . . . . . . . . . .364 -Chlordane . . . . . . . . . . . . . . . . . .349355, 367 -Chlordane . . . . . . . . . . . . . . . . . .349355, 367 -Chlordane . . . . . . . . . . . . . . . . . . . . . . . . . .352 Chlorfenvinphos . . . . . . . . . . . . . . . . . . .356357 Chloridazon . . . . . . . . . . . . . . . . . . . . . . . . . . .628 Chloroacetic acid . . . . . . . . . . . . . . . . . . . . . .370 Chloroacetonitrile . . . . . . . . . . . . . . . . .371372 2-Chloroaniline . . . . . . . . . . . . . . . .397, 411, 622 3-Chloroaniline . . . . . . . . . . . . . . . .397, 411, 622 4-Chloroaniline . . . . . . . . . . . .366, 397, 411, 622 Chlorobenzene . . . . . . .371373, 375, 378379, 399400, 406407 Chlorobenzene-d5 . . . . . . . . . . . . . . . . .378380 Chlorobenzilate . . . . . . . . . . .350352, 355, 367 2-Chlorobenzoic acid . . . . . . . . . . . . . . . . . . .644 4-Chlorobenzonitrile . . . . . . . . . . . . . . . . . . .397 2-Chlorobiphenyl . . . . . . . . . . . . . . . . . . . . . .367 1-Chlorobutane . . . . . . . . . . .371372, 405, 423 2-Chlorobutane . . . . . . . . . . . . . . . . . . . . . . .423 Chlorodibromoacetic . . . . . . . . . . . . . . . . . . .370 1-Chloro-1,1-difluoroethane . . . . . . . . . . . . .380 Chlorodifluoromethane . . . . . . . . . . . . .380, 413 2-Chloro-4,6-dinitroaniline . . . . . . . . . . . . . .411 Chloroethane . . . . . . . . .371373, 375, 378380 2-Chloroethanol . . . . . . . . . . . . . . . . . . .372, 414 bis(2-Chloroethoxy) methane . . . . . . . . . . . .366 bis(2-Chloroethyl) ether . . . . . . . . . . . . . . . .366 2-Chloroethyl vinyl ether . . . . . . . . . . . . . . . .373 Chloroform . . . . . . . . . .371373, 375379, 405, 407408, 413, 423424 1-Chloro-3-fluorobenzene . . . . . . . . . . .371, 373 1-Chlorohexane . . . . . . . . . . . . . . . . . . .372, 405 Chloro isooctane . . . . . . . . . . . . . . . . . . . . . .406 bis (2-Chloroisopropyl) ether . . . . . . . .366, 373 Chloromethane . . . . . . .371373, 375, 378380 4-Chloro-2-methylaniline . . . . . . . . . . . . . . . .411 4-Chloromethyl 2,2-dimethyl pentane . . . . .406 2-Chloro-5-methylphenol . . . . . . . . . . . . . . .369 4-Chloro-2-methylphenol . . . . . . . . . . . . . . .369 4-Chloro-3-methylphenol . . .366, 369, 412, 629 2-Chloronaphthalene . . . . . . . . . . . . . . . . . . .366 Chloroneb . . . . . . . . . . . . . . . .350352, 355, 367 2-Chloro-4-nitroaniline . . . . . . . . . . . . . . . . .411 4-Chloro-2-nitroaniline . . . . . . . . . . . . . . . . .411 1-Chloro-4-nitrobenzene . . . . . . . . . . . .399400 4-Chloro-3-nitrobenzotrifluoride . . . . . . . . . .350 4-Chloro-3-nitrotrifluoride . . . . . . . . . . .356357 Chloropentafluoroethane . . . . . . . . . . . . . . .380 Chlorpheniramine . . . . . . . . .530, 542, 545, 556 2-Chlorophenol . . . . . . . . . . .366, 369, 412, 629 4-Chlorophenol . . . . . . . . . . . . . . . . . . . . . . . .369 o-Chlorophenoxy acetic acid . . . . . . . . . . . .629 p-Chlorophenoxy acetic acid . . . . . . . . . . . .629 o-Chlorophenoxy proprionic acid . . . . . . . . .629 4-Chlorophenyl-phenyl ether . . . . . . . . . . . .366 Chloropicrin . . . . . . . . . . . . . . . . . . . . . .376377 Chloroprene . . . . . . . . . . . . . . . . . . . . . . . . . .373 2-Chloropropane . . . . . . . . . . . . . . . . . . . . . .373 2-Chloropropene . . . . . . . . . . . . . . . . . . . . . .436 3-Chloropropene (allyl chloride) . . . . . . . . . . .405 3-Chloro-1-propene (Allyl chloride) . . . . . . . . .379 3-Chloropropionitrile . . . . . . . . . . . . . . . . . . .372 Chloropropylate . . . . . . . . . . . . . . . . . . . . . . .350 4-Chlorostyrene . . . . . . . . . . . . . . . . . . .399400 2-Chloro-1,1,1,2-tetrafluoroethane . . . . . . . .380 Chlorothalonil . . . . . . . . . . . . . . . .350352, 367 2-Chlorotoluene . . .371373, 375, 399400, 407 3-Chlorotoluene . . . . . . . . . . . . . . .399400, 407 4-Chlorotoluene . .371373, 399400, 406407 Chlorotoluron . . . . . . . . . . . . . . . . . . . . . . . . .628 Chloroxuron . . . . . . . . . . . . . . . . . . . . . . . . . .628 Chlorpheniramine . . . . . . . . .415, 417, 606, 644 Chlorpothixene . . . . . . . . . . . . . . . . . . . . . . . .418 Chlorpropham . . . . . . . . . . . . . . . . . . . . .356, 367 Chlorpyrifos . . . . . . . . . . . . . . . . . . . . . .355357 Chlorpyrifos-methyl . . . . . . . . . . . . . . . .356357 Chlorsulfuron-methyl . . . . . . . . . . . . . . . . . . .630 4-Chlortestosterone-17-acetate (Clostebal) . . . .422 Chlortetracycline . . . . . . . . . . . . . . . . . .649650 Chlortoluron . . . . . . . . . . . . . . . . . . . . . . . . . .628 5--Cholestane . . . . . . . . . . . . . . . . . . . . . . .421 Cholesterol . . . . . . . . . . . . . . . . . . . . . . . . . . .421 Chrysene . . . . . . . . . . . . . . . .366368, 626627 Chrysene-d12 . . . . . . . . . . . . . . . . . . . . .366367 Chymotrypsinogen . . . . . . . . . . . . . . . . . . . . .617 Cimetidine . . . . . . . . . . . . . . . . . . . . . . . . . . .651 d-Cinchonine . . . . . . . . . . . . . . . . . . . . .526, 643 1,8-Cineol . . . . . . . . . . . . . . . .381382, 385386 Cineole . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .383 Cinerins . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .364 Cinnamaldehyde . . . . . . . . . . . . . . . . . . . . . .632 trans-Cinnamaldehyde . . . . . . . . . . . . . . . . .383 Cinnamyl cinnamate . . . . . . . . . . . . . . . . . . .632 Cinnamyl phenyl acetate . . . . . . . . . . .381382 Cinnamylacetate . . . . . . . . . . . . . . . . . . . . . .386 Cinnanzine . . . . . . . . . . . . . . . . . . . . . . . . . . .417 Ciprofloxacin . . . . . . . . . . . . . . . . . . . . . . . . .643 Citonellal . . . . . . . . . . . . . . . . . . . . . . . . . . . . .385 Citral . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .383 Citric Acid . . . . . . . . . . . . . . . . . . . . . . . . . . . .621 (R)-(+)-Citronellal . . . . . . . . . . . . . . . . . . . . . .383 Citronellal . . . . . . . . . . . . . . . . . . . .381, 384, 630 Citronellol . . . . . . . . . . . . . . . . . . . .381383, 388 Citronellyl acetate . . . . . . . . . . . . . . . . .381382 Citronellyl formate . . . . . . . . . . . . . . . . . . . . .381 Citronellyl propionate . . . . . . . . . . . . . . . . . .381 Citronellyl tiglate . . . . . . . . . . . . . . . . . .381382 cis-Citronellyl tiglate . . . . . . . . . . . . . . . . . . .383 trans-Citronellyl tiglate . . . . . . . . . . . . . . . . .383 Clemizole . . . . . . . . . . . . . . . . . . . . . . . . . . . .417 Clobazam . . . . . . . . . . . . . . . . . . . . . . . . . . . .419 Clonazepam . . . . . . . . . . . . . . . . . . . . . . . . . .419 Cloridazon . . . . . . . . . . . . . . . . . . . . . . . . . . . .628 Clotrimazole . . . . . . . . . . . . . . . . . . . . . . . . . .644 Cocaine . . . . . . . . . . . . . . . . .415416, 421, 647 Codeine . . . . . . . . . . . . .415416, 420421, 648 Coke, Diet . . . . . . . . . . . . . . . . . . . . . . . . . . . .632 Cola . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .636 Commamyl acetate . . . . . . . . . . . . . . . . . . . .383 Cool Mint Listerine . . . . . . . . . . . . . . . . . . . .633 Copaene . . . . . . . . . . . . . . . . . . . . . . . . .381382 Coprostane (5--cholestane) . . . . . . . . . . . . . .421 Corn Syrup . . . . . . . . . . . . . . . . . . . . . . . . . . .637 Coronene . . . . . . . . . . . . . . . . . . . . . . . . . . . .626 Corticosterone . . . . . . . . . . . . . . . . . . . . . . . .649 Cortisone acetate . . . . . . . . . . . . . . . . . . . . .649 Cough-cold Remedies . . . . . . . . . . . . . .563, 590 Coumaphos . . . . . . . . . . . . . . . . . . . . . .356357 Coumarin . . . . . . . . . . . . . . . . . . . . . . . .383, 634 m-Cresol . . . . . . . . . . . . . . . . . . . . .412413, 629 o-Cresol . . . . . . . . . . . . . . . . . . . . .412413, 629 p-Cresol . . . . . . . . . . . . . . . . . . . . . . . . .412413 Crimidin . . . . . . . . . . . . . . . . . . . . . . . . . . . . .628 Crotonaldehyde . . . . . . . . . . . . . . .372, 398, 630 Crotonaldehyde-DNPH . . . . . . . . . . . . . . . . .622 Crotoxyphos . . . . . . . . . . . . . . . . . . . . . .356357 Cumene . . . . . . . . .347, 371373, 375, 399401, 406, 407, 430, 435437 Cyanazine . . . . . . . . . . . . . . . . . . . .351, 367, 628 2-Cyanopyridine . . . . . . . . . . . . . . . . . . . . . . .397 3-Cyanopyridine . . . . . . . . . . . . . . . . . . . . . . .397 Cyclizine . . . . . . . . . . . . . . . . . . . . . . . . . . . . .417 Cycloate . . . . . . . . . . . . . . . . . . . . . . . . .356, 367 Cyclohexane . . . . . . . . .379, 406, 408, 424, 430, 435, 437 Cyclohexanol . . . . . . . . . . . . . . . . . . . . .394395 Cyclohexanone . . . . . . . . . . . . . . . . . . . .398, 408 n-Cyclohexylamine . . . . . . . . . . . . . . . . . . . .396 2-Cyclohexyl-4,6-dinitrophenol . . . . . . . . . . .369 Cyclohexyl methacrylate . . . . . . . . . . . . . . . .410 Cyclopentane . . . . . . . . . . . . . . . . . . . . .430, 438 Cyclopentanol . . . . . . . . . . . . . . . . . . . . . . . .394 Cyclopentanone . . . . . . . . . . . . . . . . . . . . . . .398 Cyclopentene . . . . . . . . . . . . . . . . . . . . . . . . .430 1-Cyclopentene . . . . . . . . . . . . . . . . . . . . . . .438 Cyclopentylbarbital . . . . . . . . . . . . . . . . . . . .418 Cyclopropane . . . . . . . . . . . . . . . . . . . . .426429 Cyheptamide . . . . . . . . . . . . . . . . . . . . . . . . .416 p-Cymene . . . . . . . . . . . .381, 382, 384387, 632 Cytidine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .607 Cytochrome C . . . . . . . . . . . .533, 611612, 616 Cytosine . . . . . . . . . . . . . . . . . . . . . . . . . . . . .652
D
2,4-D . . . . . . . . . . . . . . . . . . . . . . . .357358, 629 Dacthal . . . . . . . . . . . . . . . . . . . . . .350, 358, 627 Daidzen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .527 Dalapon . . . . . . . . . . . . . . . . . . . . .357358, 370 2,4-DB . . . . . . . . . . . . . . . . . . . . . . .357358, 629 DCPA . . . . . . . . . . . . . . . . . . .351352, 355, 357 4,4'-DDD . . . . . . . . . . . . . . . . . . . . .349, 351352 o,p'-DDD . . . . . . . . . . . . . . . . . . . . . . . . . . . . .355 p,p'-DDD . . . . . . . . . . . . . . . . .350, 353355, 367 4,4'-DDE . . . . . . . . . . . . . . . . . . . . .349, 351352 o,p'-DDE . . . . . . . . . . . . . . . . . . . . . . . . . . . . .355 p,p'-DDE . . . . . . . . . . . . . . . . .350, 353355, 367 4,4'-DDT . . . . . . . . . . . . . . . . . . . . .349, 351352 o,p'-DDT . . . . . . . . . . . . . . . . . . . . . . . . . . . . .355 p,p'-DDT . . . . . . . . . . . . . . . . .350, 353355, 367 DEA . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .628 Decachlorobiphenyl . . . . . . . .349, 363, 353355 Decanal . . . . . . . . . . . . . . . . . . . . .384385, 397 Decane . . . . . . . . . . . . . .348, 406, 435, 437439 1,10-Decanediol . . . . . . . . . . . . . . . . . . .403404 Decanoic acid . . . . . . . . . . . . . . . . . . . . .390, 397 Decanol . . . . . . . . . . . . . . . . . . . . . . . . . .387, 394 n-Decylamine . . . . . . . . . . . . . . . . . . . . .396397 DEF . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .367 Dehydroisoandrosterone (Prasterone) . . . . . .422 1-Dehydro-17-a-methyltestosterone (Methandrostenolone) . . . . . . . . . . . . . . . . .422 1-Dehydrotestosterone (Boldenone) . . . . . . .422
Indices
710
Compound Index
1-Dehydrotestosterone acetate . . . . . . . . . .422 1-Dehydrotestosterone benzoate . . . . . . . . .422 1-Dehydrotestosterone undecylenate . . . . .422 Delorazepam . . . . . . . . . . . . . . . . . . . . . . . . .419 Demeton-O . . . . . . . . . . . . . . . . . . . . . . .356357 Demeton-S . . . . . . . . . . . . . . . . . . . . . . .356357 Demoxepam . . . . . . . . . . . . . . . . . . . . . . . . . .419 2' Deoxycytidine . . . . . . . . . . . . . . . . . . . . . .607 2' Deoxyguanosine . . . . . . . . . . . . . . . . . . . .607 2' Deoxyinosine . . . . . . . . . . . . . . . . . . . . . . .607 n-Des . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .418 Desethylatrazine . . . . . . . . . . . . . . . . . .625, 628 Desethyldesisopropylatrazine . . . . . . . . . . . .625 Desethylterbutylazine . . . . . . . . . . . . . . . . . .628 Desipramine . . . . . . . . . . . . . . . . . . . . . .416, 650 Desisopropylatrazine . . . . . . . . . . . . . . . . . . .628 Desmethyldiazepam . . . . . . . . . . . . . . . . . . .419 DETBA . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .628 Dexamethasone . . . . . . . . . . . . . . . . . . . . . . .651 Dextrans . . . . . . . . . . . . . . . . . . . . . . . . . . . . .589 Dextromethorphan . . . . . . . . . . . . .420, 606, 647 Dextrum . . . . . . . . . . . . . . . . . . . . . . . . . . . . .618 Diacetone alcohol . . . . . . . . . . . . . . . . . . . . .408 Diacetyl . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .388 Diacetylmorphone (Heroin) . . . . . . . . . . . . . .421 Di-allate A . . . . . . . . . . . . . . . . . . . . . . . . . . . .350 Di-allate B . . . . . . . . . . . . . . . . . . . . . . . . . . . .350 2,4-Diaminoanisole . . . . . . . . . . . . . . . . . . . .411 2,4-Diaminotoluene . . . . . . . . . . . . . . . . . . . .411 3,4-Diaminotoluene . . . . . . . . . . . . . . . . . . . .397 Diamyl phthalate . . . . . . . . . . . . . . . . . . . . . .368 Diazepam . . . . . . . . . . . . . . . .415416, 419, 550 Diazinon . . . . . . . . . . . . . . . . . . . . .356357, 367 Dibenz[a,h]anthracene . . . . .366367, 626-627 1,2,3,4-Dibenzanthracene . . . . . . . . . . . . . . .368 1,2,5,6-Dibenzanthracene . . . . . . . . . . . . . . .368 Dibenzofuran . . . . . . . . . . . . . . . . . . . . .366, 368 Dibenzo-p-dioxin . . . . . . . . . . . . . . . . . . . . . .368 Dibenzothiophene . . . . . . . . . . . . . . . . . . . . .368 Dibenzyl ether . . . . . . . . . . . . . . . . . . . .381382 Dibenzyl phthalate . . . . . . . . . . . . . . . . . . . . .368 Diborane . . . . . . . . . . . . . . . . . . . . . . . . . . . . .414 Dibromoacetic acid . . . . . . . . . . . . . . . . . . . .370 Dibromoacetonitrile . . . . . . . . . . . . . . . .376377 1,2-Dibromobenzene . . . . . . . . . . . . . . . . . . .378 4,4'-Dibromobiphenyl . . . . . . . . . . . . . . . . . .351 Dibromochloromethane . . . . . . . .413, 371373, 375377, 379 1,2-Dibromo-3-chloropropane (DBCP) . . . . .350, 371373, 376377, 405 Dibromodichloromethane . . . . . . . . . . . . . . .413 Dibromodifluoromethane (Freon 12B2) . . . . .413 Dibromofluoromethane . . . . . . . . . . . . . . . . .372 Dibromomethane . . . . . . . . .350, 371373, 375, 405, 413 1,2-Dibromomethane (EDB) . . . . . . . . .371373, 376379 2,6-Dibromo-4-nitroaniline . . . . . . . . . . . . . .411 4,4-Dibromooctafluorobiphenyl . . . . . . . . . .357 2,4-Dibromophenol . . . . . . . . . . . . . . . . . . . .369 1,2-Dibromopropane . . . . . . . . . . . . . . . . . . .376 2,3-Dibromopropionic acid . . . . . . . . . .370, 376 1,2-Dibromo-1,1,2,2-tetrafluoroethane . . . . .380 , -Dibromo-m-xylene . . . . . . . . . . . . . . . . .350 Dibucaine . . . . . . . . . . . . . . . . . . . . . . . . . . . .417 Dibutylchlorendate . . . . . . . . . . . . . . . . . . . .350 tert-Dibutyl disulfide . . . . . . . . . . . . . . . . . . .434 Dibutyl ether . . . . . . . . . . . . . . . . . . . . . . . . . .410 Dibutylpentyltin . . . . . . . . . . . . . . . . . . . . . . .365 DICAMBA . . . . . . . . . . . . . . . . . . . .357358, 629 Dichlobenil . . . . . . . . . . . . . . . . . . . . . . . . . . .358 Dichlofenthion . . . . . . . . . . . . . . . . . . . .356357 Dichloroacetic acid . . . . . . . . . . . . . . . . . . . .370 Dichloroacetonitrile . . . . . . . . . . . . . . . .376377 2,4-Dichloroaniline . . . . . . . . . . . . . . . . . . . . .622 2,5-Dichloroaniline . . . . . . . . . . . . . . . . . . . . .622 2,6-Dichloroaniline . . . . . . . . . . . . . . . . . . . . .622 3,4-Dichloroaniline . . . . . . . . . . . . .397, 411, 622 1,2-Dichlorobenzene . . . . . .366, 371373, 375, 378379, 399400, 406407 1,3-Dichlorobenzene . . . . . .366, 371373, 375, 378379, 399400, 406407 1,4-Dichlorobenzene . . . . . .366, 371373, 375, 378379, 399400, 406407 1,4-Dichlorobenzene-d4 . . . . . . . . . . . .366, 372 3,3'-Dichlorobenzidine . . . . . . . . . . . . . .366, 411 3,5-Dichlorobenzoic acid . . . . . . . . . . . . . . . .357 2,3-Dichlorobiphenyl . . . . . . . . . . . . . . . . . . .367 p,p'-Dichlorobiphenyl . . . . . . . . . . . . . . . . . .355 cis-Dichlorobutene . . . . . . . . . . . . . . . .372, 373 trans-Dichlorobutene . . . . . . . . . . . . . . . . . .372 trans-1,4-Dichloro-2-butene . . . . . . . . .371, 405 Dichlorodifluoromethane (Freon 12) . . . . .371373, 378, 379380 1,1-Dichloroethane . . .371373, 375, 377379, 405 1,2-Dichloroethane . . . .371373, 377379, 405 Dichloroethane-d4 . . . . . . . . . . . . . . . . . . . . .372 1,1-Dichloroethene . . . .371373, 375, 378379, 405 cis-1,2-Dichloroethene . . . . .371373, 378379 trans-1,2-Dichloroethene . . .371373, 375, 379, 405 1,1-Dichloroethylene . . . . . . . . . . . . . . . . . . .377 1,1-Dichloro-1-fluoroethane . . . . . . . . . . . . .380 Dichlorofluoromethane . . . . . . . . . . . . .375, 380 Dichloromethane . . . . . .313, 380, 408, 413, 436 2,6-Dichloro-4-nitroaniline . . . . . . . . . . . . . .411 2,3-Dichlorophenol . . . . . . . . . . . . . . . . . . . .369 2,4-Dichlorophenol . . . . . . . .366, 369, 412, 629 2,5-Dichlorophenol . . . . . . . . . . . . . . . . . . . .369 2,6-Dichlorophenol . . . . . . . . . . . . . . . . . . . .369 3,4,-Dichlorophenol . . . . . . . . . . . . . . . . . . . .369 3,5-Dichlorophenol . . . . . . . . . . . . . . . . . . . .369 Dichloroprop . . . . . . . . . . . . . . . . . . . . .357358 1,1-Dichloropropane . . . . . . . . . . . . . . . . . . .405 1,2-Dichloropropane . . .371373, 375, 378379, 405 1,3-Dichloropropane . . . . . . . . . . .371373, 405 2,2-Dichloropropane . . . . . . .371373, 375, 405 1,3-Dichloro-2-propanol . . . . . . . . . . . . . . . . .372 1,1-Dichloro-2-propanone . . . . . . .371, 376377 1,1-Dichloropropene . . . . . . . . . . . . . . .371373 cis-1,2-Dichloropropene . . . . . . . . . . . . . . . .405 cis-1,3-Dichloropropene . . . . . . . .371373, 375, 378379, 405 trans-1,2-Dichloropropene . . . . . . . . . . . . . .405 trans-1,3-Dichloropropene . . . . . .371373, 375, 378379, 405 Dichlorotetrafluoroethane . . . . . . . . . . . . . . .379 1,2-Dichloro-1,1,2,2-tetrafluoroethane, (Freon 114) . . . . . . . . . . . . . . . . . . . .378, 380 2,2-Dichloro-1,1,1-trifluoroethane . . . . . . . .380 Dichlorvos . . . . . . . . . . . . . . . . . . .356357, 367 Dicrotophos . . . . . . . . . . . . . . . . . . . . . .356357 Dicyclohexyl phthalate . . . . . . . . . . . . . . . . .368 Dicyclohexylamine . . . . . . . . . . . . . . . .396397 Dieldrin . . . . . . . . . . . . . . . . . . . . . .349355, 367 Diesel Fuel . . . . . . . . . . . . . . . . . . . . . . . . . . .348 Diethylamine . . . . . . . . . . . . . . . . . . . . . . . . .397 2,6-Diethylaniline . . . . . . . . . . . . . . . . . .397, 622 N,N-Diethylaniline . . . . . . . . . . . . . . . . . . . . .397 1,2-Diethylbenzene . . . . . . . . . . . . . . . . . . . .439 1,3-Diethylbenzene . . . . . . . . . . . . . . . . . . . .438 1,4-Diethylbenzene . . . . . . . . . . . . . . . . . . . .439 Diethylbenzene . . . . . . . . . . . . . . . . . . . . . . .435 Diethylene glycol . . . . . . . . . . . . . . . . . . . . .403404 Diethylene glycol monobutyl ether . . . . . .403404 Diethylene glycol monoethyl ether . . . . . .403404 Diethylene glycol monomethyl ether . . . .403404 Diethylenetriamine . . . . . . . . . . . . . . . . . . . .396397 Diethyl ether . . . . . . . . . . . . .371, 414, 423, 436 1,2-Diethyl-4-ethylbenzene . . . . . . . . . . . . . .438 1,3-Diethyl-5-ethylbenzene . . . . . . . . . . . . . .438 Diethyl phthalate . . . . . . . . . . . . . .366368, 383 Diethylstilbestrol . . . . . . . . . . . . . . . . . . . . . .527 Diethyl sulfide . . . . . . . . . . . . . . . . . . . . . . . .432 Diethyltryptamine . . . . . . . . . . . . . . . . . . . . .420 1,4-Difluorobenzene . . . . . . . . . . .371, 378380 1,1-Difluoroethane . . . . . . . . . . . . . . . . . . . . .380 Difolotan . . . . . . . . . . . . . . . . . . . . . . . . . . . . .358 Diglyme (diethylene glycol dimethyl ether) . . .403 Dihexyl phthalate . . . . . . . . . . . . . . . . . . . . . .368 9,10-Dihydroanthracene . . . . . . . . . . . . . . . .368 Dihydrocarvone . . . . . . . . . . . . . . . . . . . . . . .386 trans-Dihydrocarvyl . . . . . . . . . . . . . . . . . . . .386 Dihydrocodeine . . . . . . . . . . . . . . . . . . . . . . .420 Dihydropentaborane . . . . . . . . . . . . . . . . . . .414 Dihydroquinine . . . . . . . . . . . . . . . . . . . . . . . .649 Dihydroxybenzyl amine . . . . . . . . . . . . . . . . .645 2,3 Dihydroxybiphenyl diol . . . . . . . . . . . . . .623 Dihydroxyphenyl acetic acid . . . . . . . . . . . . .645 Dihydroxyphenylalanine . . . . . . . . . . . . . . . .645 Diisobutyl phthalate . . . . . . . . . . . . . . . . . . .368 Diisopropylamine . . . . . . . . . . . . . . . . . . . . . .397 Diisopropyl ether (DIPE) . . . . . . . . . . . .347, 372 1,3-Diisopropylbenzene . . . . . . . . .399400, 407 1,4-Diisopropylbenzene . . . . . . . . .399400, 407 Diltiazem . . . . . . . . . . . . . . . . . . . . . . . . . . . . .557 Dimefuron . . . . . . . . . . . . . . . . . . . . . . . . . . . .628 Dimenhydrinate . . . . . . . . . . . . . . . . . . .415, 417 Dimethoate . . . . . . . . . . . . . . . . . . . . . .356357 3,3'-Dimethoxybenzidine . . . . . . . . . . . . . . . .411 1,2-Dimethoxyethane . . . . . . . . . . . . . . . . . .436 Dimethylamine . . . . . . . . . . . . . . . . . . . . . . . .396 Dimethylamphetamine . . . . . . . . . . . . . . . . .416 2,4-Dimethylaniline . . . . . . . . . . . . . . . . . . . .397 2,6-Dimethylaniline . . . . . . . . . . . .397, 411, 622 7,12-Dimethylbenz . . . . . . . . . . . . . . . . . . . . .368 1,2-Dimethylbenzene . . . . . . . . . . . . . . . . . . .383 N,N-Dimethylbenzylamine . . . . . . . . . . . . . .397 2,2-Dimethylbutane . . . . . . . . . . . . . . . .430, 438 2,3-Dimethylbutane . . . . . . . . . . . . . . . .430, 438 Dimethyldiselenide . . . . . . . . . . . . . . . . . . . .389 Dimethyldisulfide . . . . . . . . . . . . . . . . . . . . . .389 Dimethylformamide (DMF) . . . . . . . . . .408, 424 2,6-Dimethylhept-5-enal . . . . . . . . . . . . . . . .381 2,6-Dimethylhept-5-enal (MelonalTM) . . . .382 2,2-Dimethylhexane . . . . . . . . . . . . . . . . . . . .371 2,6-Dimethylnaphthalene . . . . . . . . . . . . . . .368
Indices
711
Compound Index
2,2-Dimethylpentane . . . . . . . . . . . . . . . . . . .438 2,3-Dimethylpentane . . . . . . . . . . . . . . . . . . .430 2,4-Dimethylpentane . . . . . . . . . . . . . . .430, 438 Dimethylpentyltin . . . . . . . . . . . . . . . . . . . . . .365 3,6-Dimethylphenanthrene . . . . . . . . . . . . . .368 2,3-Dimethylphenol . . . . . . . . . . . . . . . . . . . .369 2,4-Dimethylphenol . . . . . . . . . . . .366, 369, 412 2,5-Dimethylphenol . . . . . . . . . . . . . . . . . . . .369 2,6-Dimethylphenol . . . . . . . . . . . . . . . . . . . .369 3,4-Dimethylphenol . . . . . . . . . . . . . . . . . . . .369 Dimethylphthalate . . . . . . . . . . . . . . . . .366-368 Dimethyl selenide . . . . . . . . . . . . . . . . . . . . .389 Dimethyl sulfide . . . . . . . . . . . . . . .380, 432, 434 Dimethyltetrasulfide . . . . . . . . . . . . . . . . . . .384 2,5-Dimethylthiophene . . . . . . . . . . . . . . . . .432 Dimethyl thiophenes . . . . . . . . . . . . . . . . . . .434 Dimethyltrisulfide . . . . . . . . . . . . . . . . .384, 389 Dimethyltryptamine . . . . . . . . . . . . . . . . . . . .420 Dimethylxanthine . . . . . . . . . . . . . . . . . . . . . .545 Di-n-butylphthalate . . . . . . . . . . . . . . . .366368 4,6-Dinitro-2-methylphenol . . . . . . . . . . . . . .366 2,4-Dinitroaniline . . . . . . . . . . . . . . . . . . . . . .411 1,3-Dinitrobenzene . . . . . . . . . . . . . . . .623624 2,2'-Dinitrobiphenyl . . . . . . . . . . . . . . . . . . . .368 2,7-Dinitrofluorene . . . . . . . . . . . . . . . . . . . . .368 1,3-Dinitronaphthalene . . . . . . . . . . . . . . . . .368 1,5-Dinitronaphthalene . . . . . . . . . . . . . . . . .368 2,4-Dinitrophenol . . . . . . . . . .366, 369, 412, 629 2,5-Dinitrophenol . . . . . . . . . . . . . . . . . . . . . .369 2,4-Dinitrotoluene . . . . . . . . .366367, 623624 2,6-Dinitrotoluene . . . . . . . . .366367, 623624 Di-n-octylphthalate . . . . . . . . . . . . . . . .366, 368 Dinonyl phthalate . . . . . . . . . . . . . . . . . . . . . .368 Dinoseb . . . . . . . . . . . . . . . . . . . . .357358, 369 Diols . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .404 1,4-Dioxane . . . . . .372, 379, 403, 408, 424, 435 Dioxathion . . . . . . . . . . . . . . . . . . . . . . .356357 1,3-Dioxolane . . . . . . . . . . . . . . . . . . . . . . . . .403 Dioxins . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .360 DIPA . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .628 DIPE . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .436 Diphenamid . . . . . . . . . . . . . . . . . .356, 358, 367 Diphenhydramine . . . . . . . . . . . . .417, 545, 649 Diphenylamine . . . . . . . . . . . . . . . . . . . .623624 Diphenylaniline . . . . . . . . . . . . . . . . . . . . . . .397 Diphenyl isophthalate . . . . . . . . . . . . . . . . . .368 Diphenyl oxide . . . . . . . . . . . . . . . . . . . .381382 Diphenyl phthalate . . . . . . . . . . . . . . . . . . . .368 9,10-Diphenylanthracene . . . . . . . . . . . . . . .368 Diphenylpyraline . . . . . . . . . . . . . . . . . . . . . .417 Dipropenyldisulfide . . . . . . . . . . . . . . . . . . . .384 Dipropyl Phthalate . . . . . . . . . . . . . . . . . . . . .537 Dipropyldisulfide . . . . . . . . . . . . . . . . . .384, 389 Dipropylene glycol . . . . . . . . . . . . . . . . .403404 Dipropyl sulfide . . . . . . . . . . . . . . . . . . . . . . .389 Dipropyltrisulfide . . . . . . . . . . . . . . . . . . . . . .389 Dipyridamole . . . . . . . . . . . . . . . . . . . . . . . . .557 Diquat . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .627 Disopyramide . . . . . . . . . . . . . . . . . . . . . . . . .649 Disulfoton . . . . . . . . . . . . . . . . . . . .356357, 367 Diuron . . . . . . . . . .358, 596, 624625, 627629 DMF (dimethylformamide) . . . . . . . . . . . . . .409 DMSO (dimethyl sulfoxide) . . . . . . . . . .409, 423 DNA . . . . . . . . . . . . . . . . . . . . . . . . . . . .607608 DNPH (reagent) . . . . . . . . . . . . . . . . . . . . . . .630 cis-13,16-Docosadienoic acid methyl ester (C22:2) . . . . . . . . . . . . . . . . .392 cis-4,7,10,13,16,19-Docosahexaenoic acid methyl ester (C22:6n3) . . . . . . . . . . . . . . .392 Docusane . . . . . . . . . . . . . . . . . . . . . . . . . . . .348 Dodecahydrotriphenylene . . . . . . . . . . . . . . .368 -Dodecalactone . . . . . . . . . . . . . . . . . .381382 Dodecane . . . . . . . . . . . .348, 406, 435, 437439 Dodecanoic acid . . . . . . . . . . . . . . . . . . . . . .390 Dodecanol . . . . . . . . . . . . . . . . . . . . . . .381382 Dodecenal . . . . . . . . . . . . . . . . . . . . . . . . . . .385 Dopamine . . . . . . . . . . . . . . . . . . . . . . . . . . . .645 n-Dotriacontane . . . . . . . . . . . . . . . . . . . . . . .439 Doxepine . . . . . . . . . . . . . . . . . . . .605606, 650 Doxycycline . . . . . . . . . . . . . . . . . . . . . .649650 Doxylamine . . . . . . . . . . . . . .415, 417, 542, 545 2,4-DP . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .629 Droperidol . . . . . . . . . . . . . . . . . . . . . . . . . . . .420 Dursban (Chlorpyrifos) . . . . . . . . . . . . . . . . .367 Ethion . . . . . . . . . . . . . . . . . . . . . . . . . . .356357 Ethoprop . . . . . . . . . . . . . . . . . . . . .356357, 367 Ethosuximide . . . . . . . . . . . . . . . . . . . . . . . . .417 Ethotoin . . . . . . . . . . . . . . . . . . . . . . . . . . . . .418 Ethoxyethanol . . . . . . . . . . . . . . . . . . . . . . . .395 2-Ethoxyethanol (Cellosolve) . . . . . . . .394395 2-Ethoxyethyl acetate . . . . . . . . . . . . . .395, 402 bis(2-Ethoxyethyl) phthalate . . . . . . . . . . . . .368 bis(2-Ethylhexyl) phthalate . . . . . . . . . .366368 Ethyl acetate . . . . . . . . .372, 379, 382, 388, 402, 407408, 410, 423424 Ethyl acetate Acetal . . . . . . . . . . . . . . . . . . .388 Ethyl acrylate . . . . . . . . . . . . . . . . .402, 409410 Ethyl alcohol . . . . . . . . . . . . . . . . . . . . . . . . . .395 Ethylbenzene . . . . . . . . . . .347348, 371373, 375, 378379, 399401, 406407, 423, 430, 435439 Ethylbenzene-d10 . . . . . . . . . . . . . . . . . . . . .439 Ethyl benzoate . . . . . . . . . . . . . . . . . . . .388, 402 2-Ethyl-1-butanol . . . . . . . . . . . . . . . . . . . . . .395 Ethyl butyrate . . . . . . . . . . . . .381382, 388, 402 Ethyl caproate . . . . . . . . . . . . . . . . . . . . . . . .402 Ethyl cellosolve . . . . . . . . . . . . . . . . . . . . . . .408 Ethyl cinnamate . . . . . . . . . . . . . . . . . . . . . . .632 Ethyl decanoate . . . . . . . . . . . . . . . . . . .381382 Ethyl disulfide . . . . . . . . . . . . . . . . . . . . . . . . .432 Ethyl dodecanoate . . . . . . . . . . . . . . . . .381382 Ethyl ether . . . . . . . . . . . . . . .372, 403, 408, 424 Ethylene . . . . . . . . . . . . .426428, 430431, 433 Ethylenediamine . . . . . . . . . . . . . . . . . .396397 Ethylene glycol . . . . . . .394, 403404, 414, 423 Ethylene glycol monobutyl ether . . . . .403404 Ethylene glycol monoethyl ether . . . . .403404 Ethylene glycol monomethyl ether . . .403404 Ethylene glycol phenyl ether . . . . . . . . . . . .404 Ethylene glycol/monoacetate . . . . . . . . . . .395 Ethylene glycol/monoformate . . . . . . . . . . .395 Ethylene oxide . . . . . . . . . . . . . . . .395, 414, 436 Ethyl formate . . . . . . . . .388, 395, 402, 407, 423 Ethyl heptanoate . . . . . . . . . . . . . . . . . .381382 Ethyl hexadecanoate . . . . . . . . . . . . . . .381382 Ethyl hexanoate . . . . . . . . . . . . . . . . . . .381382 2-Ethyl-1-hexanol . . . . . . . . . . . . . . . . . .387, 394 bis (2-Ethylhexyl) adipate . . . . . . . . . . . . . . . . .367 Ethyl isovalerate . . . . . . . . . . . . . . . . . .381382 Ethyl mercaptan . . . . . . . . . . . . . . . . . . .432434 Ethyl methacrylate . . . . . . . . . . . .371372, 410 2-Ethyl-6-methylaniline . . . . . . . . . . . . . . . . .622 Ethyl methyl sulfide . . . . . . . . . . . . . . . . . . . .432 Ethylmorphine . . . . . . . . . . . . . . . . . . . . . . . .420 Ethyl nonanoate . . . . . . . . . . . . . . . . . . .381382 Ethyl octadecanoate . . . . . . . . . . . . . . .381382 Ethyl octanoate . . . . . . . . . . . . . . . . . . .381382 Ethyl paraben . . . . . . . . . . . . . . . . . . . . . . . . .638 Ethyl parathion . . . . . . . . . . . . . . . . . . . .356357 Ethyl pentadecanoate . . . . . . . . . . . . . .381382 Ethyl pentanoate . . . . . . . . . . . . . . . . . .381382 -Ethylphenethyl alcohol . . . . . . . . . . . . . . .394 -Ethylphenethyl alcohol . . . . . . . . . . . . . . .394 2-Ethylphenol . . . . . . . . . . . . . . . . . . . . . . . . .413 4-Ethylphenol . . . . . . . . . . . . . . . . . . . . . . . . .413 Ethyl-3-phenyl oxiran carboxylate . . . . . . . .388 Ethyl propionate . . . . . . . . . .381382, 402, 410 Ethyl-tert-butyl ether (ETBE) . . . . . . . .347, 372 Ethyl tetradecanoate . . . . . . . . . . . . . . .381382 2-Ethylthiophene . . . . . . . . . . . . . . . . . . . . . .432
E
Ecgonine methylester . . . . . . . . . . . . . . . . . .647 Econazole . . . . . . . . . . . . . . . . . . . . . . . . . . . .644 cis-11,14-Eicosadienoic acid methyl ester (C20:2) . . . . . . . . . . . . . . . . . . . . . . . . . . . . .392 Eicosane . . . . . . . . . . . . . . . . . . . . .348, 381, 439 cis-5,8,11,14,17-Eicosapentaenoic acid methyl ester (C20:5n3) . . . . . . . . . . . . . . . . . . . . . .392 cis-11,14,17-Eicosatrienoic acid methyl ester (C20:3n3) . . . . . . . . . . . . . . . . . . . . . . . . . . .392 cis-8,11,14-Eicosatrienoic acid methyl ester (C20:3n6) . . . . . . . . . . . . . . . . . . . . . . . . . . .392 cis-11-Eicosenoic acid methyl ester (C20:1) . . . . . . . . . . . . . . . . . . . . . . . . . . . . .392 Elaidic acid methyl ester (C18:1n9t) . . . . . . .392 Endo-MGK 264 . . . . . . . . . . . . . . . . . . . . . . . .364 Endosulfans . . . . . . . . . . . . . . . . . .349355, 367 Endrin . . . . . . . . . . . . . . . . . . . . . . .349355, 367 Endrin aldehyde . . . . . . . . . . . . . . .349355, 367 Endrin ketone . . . . . . . . . . . .349350, 353355 Ephedrine . . . . . . . . . . . . . . . . . . . . . . . . . . . .416 Epiandrosterone (trans-androsterone) . . . . . . .421 Epicatechin . . . . . . . . . . . . . . . . . . . . . . . . . . .635 Epicatechin gallate . . . . . . . . . . . . . . . . . . . .635 Epichlorohydrin . . . . . . . . . . . . . . . . . . .372, 403 Epigallocatechin . . . . . . . . . . . . . . . . . . . . . . .635 Epigallocatechin gallate . . . . . . . . . . . . . . . .635 Epinephrine . . . . . . . . . . . . . . . . . . . . . . . . . .645 Epi-tetracycline . . . . . . . . . . . . . . . . . . .649650 EPN . . . . . . . . . . . . . . . . . . . . . . . . . . . . .356357 Epoxy Resins . . . . . . . . . . . . . . . . . . . . . . . . .618 EPTC . . . . . . . . . . . . . . . . . . . . . . . .356, 358, 367 Equal Sweetener . . . . . . . . . . . . . . . . . . . . . .632 Erucic acid methyl ester (C22:1n9) . . . . . . .392 Erythrosine . . . . . . . . . . . . . . . . . . . . . . .634, 638 Esters . . . . . . . . . . . . . . . . . . . . . . . . . . .402403 17--Estradiol . . . . . . . . . . . . . . . . . . . . . . . .421 -Estradiol . . . . . . . . . . . . . . . . . . . . . . . . . . .421 Estriol . . . . . . . . . . . . . . . . . . . . . . . . . . .421, 527 Estrone . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .421 ETBE . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .436 Ethane . . . . . . . . . . . . . . . . . .425428, 430431, 422424, 436438 Ethchlorvynol . . . . . . . . . . . . . . . . . . . . . . . . .420 Ethiazide . . . . . . . . . . . . . . . . . . . . . . . . .576, 645 Ethinamate . . . . . . . . . . . . . . . . . . . . . . . . . . .420
Indices
712
Compound Index
Ethyl thiophenes . . . . . . . . . . . . . . . . . . . . . .434 4-Ethyltoluene . . . . . . . . . . . . . . . . . . . . . . . .379 m-Ethyltoluene . . . . . . . . . . . . . . . . . . . . . . . .435 p-Ethyltoluene . . . . . . . . . . . . . . . . . . . . . . . .435 p/m-Ethyltoluene . . . . . . . . . . . . . . . . . . . . .436 Ethyl undecanoate . . . . . . . . . . . . . . . . .381382 Ethyl valerate . . . . . . . . . . . . . . . . . . . . . . . . .402 Ethyl-vanillin . . . . . . . . . . . . . . . . . . . . . . . . . .634 Ethyl vinyl ether . . . . . . . . . . . . . . . . . . . . . . .403 Etofumesate . . . . . . . . . . . . . . . . . . . . . . . . . .629 Etridiazole . . . . . . . . . . . . . . . . . . . .351352, 355 Eugenol . . . . . . . . . . . . . . . . . . . . . . . . . .383, 632 Eugenyl acetate . . . . . . . . . . . . . . . . . . .381382 Eugenyl methyl ether . . . . . . . . . . . . . . . . . . .382 Evernyl . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .381 Exo-MGK 264 . . . . . . . . . . . . . . . . . . . . . . . . .364 Explosives . . . . . . . . . . . . . . . . . . . . . . .623624 Freon 11 (Trichlorofluoromethane) . . . . . . . .378 Freon 12 (Dichlorodifluoromethane) . . . . .371-373, 378-380, 413 Freon 12B2 . . . . . . . . . . . . . . . . . . . . . . . . . . .413 Freon 21 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .413 Freon 22 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .413 Freon 113 (1,1,2-Trichloro-1,2,2-trifluoroethane) . . .378, 413 Freon 114 (1,2-Dichloro-1,1,2,2-tetrafluoroethane) . .378, 413 Freons . . . . . . . . . . . . . . . . . . . . . . . . . . .380, 413 Fructose . . . . . . . . . . . . .389, 592, 633, 635, 637 Fruit Bar . . . . . . . . . . . . . . . . . . . . . . . . . . . . .637 Fucitol . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .388 Fumaric Acid . . . . . . . . . . . . . . . . . . . . . . . . .621 Furans . . . . . . . . . . . . . . . . . . . . . . . . . . .360, 403 Furfural . . . . . . . . . . . . . . . . . . . . . .381382, 398 Furfuryl alcohol . . . . . . . . . . . . . . . . . . . . . . .394 Halothane . . . . . . . . . . . . . . . . . . . . . . . . . . . .414 1,2,3,4,6,7-HeCDD . . . . . . . . . . . . . . . . . . . . .360 1,2,3,4,6,9-HeCDD . . . . . . . . . . . . . . . . . . . . .360 1,2,3,4,7,8-HeCDD . . . . . . . . . . . . . . . . . . . . .360 1,2,3,6,7,8-HeCDD . . . . . . . . . . . . . . . . . . . . .360 1,2,3,7,8,9-HeCDD . . . . . . . . . . . . . . . . . . . . .360 1,2,4,6,7,9-/1,2,4,6,8,9-HeCDD . . . . . . . . . . .360 1,2,3,4,7,8/1,2,4,6,8,9-HeCDF . . . . . . . . . . . .360 1,2,3,4,8,9-HeCDF . . . . . . . . . . . . . . . . . . . . . .360 1,2,3,6,7,8-HeCDF . . . . . . . . . . . . . . . . . . . . . .360 1,2,3,7,8,9-HeCDF . . . . . . . . . . . . . . . . . . . . . .360 1,3,4,6,7,9-HeCDF . . . . . . . . . . . . . . . . . . . . . .360 2,3,4,6,7,8-HeCDF . . . . . . . . . . . . . . . . . . . . . .360 Heneicosane . . . . . . . . . . . . . . . . . . . . . . . . .381 Heneicosanoic acid methyl ester (C21:0) . . . .392 Heparin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .605 1,7-Hepatanediol . . . . . . . . . . . . . . . . . . . . . .404 2,2',3,3',4,4',6-Hepta . . . . . . . . . . . . . . . . . . .367 Heptachlor . . . . . . . . . . . . . . . . . . .349355, 367 Heptachlor epoxide . . . . . . . . . . . .349355, 367 Heptadecane . . . . . . . . . . . . . . . . . . . . . . . . .364 n-Heptadecane . . . . . . . . . . . . . . . . . . . . . . . .439 Heptadecanoic acid methyl ester (C17:0) . .392 Heptadec-1-ene . . . . . . . . . . . . . . . . . . . . . . .381 cis-10-Heptadecenoic acid methyl ester (C17:1) . . . . . . . . . . . . . . . . . . . . . . .392 Heptaldehyde . . . . . . . . . . . . . . . . . . . . . . . . .630 Heptanal . . . . . . . . . . . . . . . . . . . . . . . . .397398 Heptane . . . . . . . . . . . . .401, 406, 423, 425, 435 n-Heptane . . . . . . .379, 408, 424, 430, 437439 1,7-Heptanediol . . . . . . . . . . . . . . . . . . .403404 1-Heptanethiol . . . . . . . . . . . . . . . . . . . . . . . .432 Heptanoic acid . . . . . . . . . . . . . . . .390, 395, 397 1-Heptanol . . . . . . . . . . . . . . . . . . . . . . .394395 2-Heptanol . . . . . . . . . . . . . . . . . . . . . . . . . . .394 3-Heptanol . . . . . . . . . . . . . . . . . . . . . . . . . . .394 2-Heptanone . . . . . . . . . . . . . . . . . . . . . . . . . .398 3-Heptanone . . . . . . . . . . . . . . . . . . . . . . . . . .398 4-Heptanone . . . . . . . . . . . . . . . . . . . . . . . . . .398 cis-4-Hepten-1-ol . . . . . . . . . . . . . . . . . . . . . .394 trans-2-Hepten-1-ol . . . . . . . . . . . . . . . . . . . .394 n-Heptylamine . . . . . . . . . . . . . . . . . . . . . . . .396 Herbicides . . . . . . . . . . .357359, 624625, 627 Hercolyn D . . . . . . . . . . . . . . . . . . . . . . . . . . .381 Heroin . . . . . . . . . . . . . . . . . . . . . . .415416, 420 Hesperidine . . . . . . . . . . . . . . . . . . . . . . . . . .633 Hexabromobenzene (HBB) . . . . . . . . . . . . . . .355 Hexachlorobenzene . . .350352, 355, 366367 2,2',4,4',5,6'-Hexachlorobiphenyl . . . . . . . . .367 Hexachlorobutadiene . . . . . . . . . .366, 371373, 375, 379, 405 Hexachloro-1,3-butadiene . . . . . . . . . . . . . . .378 Hexachlorocyclopentadiene . . . . . . . .351352, 366367, 405 Hexachloroethane . . . . . . . . .366, 371372, 405 Hexachloropentadiene . . . . . . . . . . . . . . . . .350 Hexacosane . . . . . . . . . . . . . . . . . . . . . . . . . .348 n-Hexadecane . . . . . . . . . . . . . . . . . . . .348, 439 Hexadecanoic acid . . . . . . . . . . . . . . . . . . . .390 Hexamethylphosphoramide . . . . . . . . .356357 Hexanal . . . . . . . . . . . . . . . . . . . . . . . . .397398 Hexanaldehyde-DNP . . . . . . . . . . . . . . . . . . .622 Hexane . . . . . . . . . . . . . .371, 406407, 423, 436 n-Hexane . . . .379, 408, 424427, 430, 437439 1,6-Hexanediol . . . . . . . . . . . . . . . . . . . .403404
F
Famotidine . . . . . . . . . . . . . . . . . . . . . . . . . . .651 Famphur . . . . . . . . . . . . . . . . . . . . . . . . .356357 FAMES . . . . . . . . . . . . . . . . . . . . . . . . . .390391 Faneosolacetate . . . . . . . . . . . . . . . . . . . . . . .386 trans- -Farnesene . . . . . . . . . . . . . . . . . . . .386 Farnesol . . . . . . . . . . . . . . . . . . . . . . . . . . . . .383 Fatty acid methyl esters (FAMES) . . . . .390393 Fatty Acids . . . . . . . . . . . . . . . . . . . . . . . . . . .638 Fatty Acids, free . . . . . . . . . . . . . . . . . . . . . . .389 FD&C Colors . . . . . . . . . . . . . . . . . . . . . . . . . .634 Fenamiphos . . . . . . . . . . . . . . . . . . . . . .356, 367 Fenarimol . . . . . . . . . . . . . . . . . . . . . . . .356, 367 Fenchone . . . . . . . . . . . . . . . . . . . . . . . . . . . .383 Fenchyl acetate . . . . . . . . . . . . . . . . . . . . . . .381 Fenfluramine . . . . . . . . . . . . . . . . . . . . . . . . .604 Fenitrothion . . . . . . . . . . . . . . . . . . . . . .356357 Fenoprofen . . . . . . . . . . . . . . . . . . . . . . . . . . .644 Fensulfothion . . . . . . . . . . . . . . . . . . . . .356357 Fentanyl . . . . . . . . . . . . . . . . . . . . .416, 419420 Fenthion . . . . . . . . . . . . . . . . . . . . . . . . .356357 Fenuron . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .628 Fig Extract . . . . . . . . . . . . . . . . . . . . . . . . . . . .639 Flunarizine . . . . . . . . . . . . . . . . . . . . . . . . . . .557 Florazone (isomers) . . . . . . . . . . . . . . . .381382 Flunitrazepam . . . . . . . . . . . . . . . . . . . . . . . . .419 Fluoranthene . . . . . . . . . . . . .366, 368, 626627 Fluorene . . . . . . . . . . . . . . . . .366368, 626627 3-Cl-4-Fluoroaniline . . . . . . . . . . . . . . . . . . . .622 Fluorobenzene . . . . . . . .371373, 375, 399400 2-Fluorobenzoic acid . . . . . . . . . . . . . . . . . . .644 3-Fluorobenzoic acid . . . . . . . . . . . . . . . . . . .644 2-Fluorobiphenyl . . . . . . . . . . . . . . . . . . . . . .366 Fluorocytosine . . . . . . . . . . . . . . . . . . . . . . . .652 2-Fluorophenol . . . . . . . . . . . . . . . . . . . . . . . .366 Fluoxetine . . . . . . . . . . . . . . . . . . . . . . . .576, 646 Flurazepam . . . . . . . . . . . . . . . . . . . . . . .416, 419 Fluridone . . . . . . . . . . . . . . . . . . . . .356, 358, 367 Folic acid . . . . . . . . . . . . . . . . . . . . . . . .641642 Folpet . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .640 Fonofos . . . . . . . . . . . . . . . . . . . . . . . . . .356357 Formaldehyde . . . . . . . . . . . .379380, 399, 630 Formaldehyde DNPH . . . . . . . . . . . . . . .399, 622 Formaldehyde-PFBHA . . . . . . . . . . . . . . . . . .399 Formic acid . . . . . . . . . . . . . . . . . . . . . . .390, 395 Frambione (raspberry ketone) . . . . . . . . .381382 Free Fatty Acids . . . . . . . . . . . . . . . . . . . . . . .389
G
Galactitol . . . . . . . . . . . . . . . . . . . . . . . . . . . .388 Galactose . . . . . . . . . . . . . . . . . . . . . . . .389, 637 Gallic acid . . . . . . . . . . . . . . . . . . . . . . . . . . . .555 Gas, Natural . . . . . . . . . . . . . . . . . . . . . . . . .425 Gas, Refinery . . . . . . . . . . . . . . . . . . . . . . . . .431 Gases, Noble . . . . . . . . . . . . . . . . . . . . . . . . .425 Gases, Permanent . . . . . . . . . . . . . . . . . . . . .425 Gasoline . . . . . . . . . . . . .348, 436437, 439440 Genistein . . . . . . . . . . . . . . . . . . . . . . . . . . . .527 Gentisic acid . . . . . . . . . . . . . . . . . . . . . . . . . .555 Geranial . . . . . . . . . . . . .381382, 384386, 388 trans-Geraniol . . . . . . . . . . . . . . . . . . . . . . . .383 Geranyl acetate . . . . . . . . . . . . . . . . . . .381382 Geranyl butyrate . . . . . . . . . . . . . . . . . .381382 Geranyl formate . . . . . . . . . . . . . . . . . . .381382 Geranyl tiglate . . . . . . . . . . . . . . . . . . . .381382 Geranyl-2-methyl valerate . . . . . . . . . . . . . . .381 Geranylacetate . . . . . . . . . . . . . . . . . . . .384, 386 Germacrene-D . . . . . . . . . . . . . . . . . . . .385386 Gesatamine (aka Atraton) . . . . . . . . . . . . . . .367 Glucitol . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .388 Glucose . . . . . . . . . . . . . . . . . . . . . .592, 633, 635 -D-Glucose . . . . . . . . . . . . . . . . . . . . . . . . . .389 -D-Glucose . . . . . . . . . . . . . . . . . . . . . . . . . .389 Glutamic acid (Glu) . . . . . . . . . . . . . . . . . . . .604 Glutethimide . . . . . . . . . . . . . . . . . . . . . .416, 420 Glyburide . . . . . . . . . . . . . . . . . . . . . . . . . . . .651 Glycidol . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .394 Glycine (Gly) . . . . . . . . . . . . . . . . . . . . . . . . . .604 Glycine-tyrosinedipeptide (gly-tyr) . . . . . . . .612 Glyceryl Guaicolate . . . . . . . . . . . . . . . . . . . .590 Glycols . . . . . . . . . . . . . . . . . . . . . . . . . .403404 Glyme (propylene glycol dimethyl ether) . . .403 3,7-Guaiadiene . . . . . . . . . . . . . . . . . . . . . . . .381 Guanine . . . . . . . . . . . . . . . . . . . . . . . . . . . . .652 Guanosine . . . . . . . . . . . . . . . . . . . . . . . . . . .607 Guanosine mono phosphate (GMP) . . . . . . .608 Gum, Chewing, sugar-free . . . . . . . . . . . . . .636
H
Hydrogen sulfide . . . . . . . . . . . . . .414, 425, 434 Halazepam . . . . . . . . . . . . . . . . . . . . . . . . . . .419 Hallucinogens . . . . . . . . . . . . . . . . . . . . . . . .420 Halocarbons . . . . . . . . . . . . . . . . . . . . . .380, 413 Haloperidol . . . . . . . . . . . . . . . . . . . . . . . . . . .420
Indices
713
Compound Index
1-Hexanethiol . . . . . . . . . . . . . . . . . . . . . . . . .432 Hexanoic acid . . . . . . . . . . . . . . . . . . . . . . . . .397 1-Hexanol . . . . . . . . . . . . . . . . . . . . . . . .394395 2-Hexanol . . . . . . . . . . . . . . . . . . . . . . . . . . . .394 3-Hexanol . . . . . . . . . . . . . . . . . . . . . . . . . . . .394 Hexanol . . . . . . . . . . . . . . . . . . . . . . . . .381382 2-Hexanone . . . . . . . . . . . . . . . . . .371, 379, 398 3-Hexanone . . . . . . . . . . . . . . . . . . . . . . . . . .398 n-Hexatriacontane . . . . . . . . . . . . . . . . . . . . .439 Hexazinon . . . . . . . . . . . . . . . . . . . . . . . .625, 628 Hexazinone . . . . . . . . . . . . . . . . . . .356, 358, 367 cis-2-Hexene . . . . . . . . . . . . . . . . . . . . . . . . .430 trans-2-Hexene . . . . . . . . . . . . . . . . . . . . . . .430 cis-2-Hexen-1-ol . . . . . . . . . . . . . . . . . . . . . . .394 cis-3-Hexen-1-ol . . . . . . . . . . . . . . . . . . . . . . .394 Hexobarbital . . . . . . . . . . . . . . . . . . . . . .415, 418 Hexobarbitol . . . . . . . . . . . . . . . . . . . . . . . . . .646 Hexogen (RDX) . . . . . . . . . . . . . . . . . . .623624 Hexyl . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .624 Hexyl acetate . . . . . . . . . . . . . . . . . . . . .381382 n-Hexylamine . . . . . . . . . . . . . . . . . . . . . . . . .396 Hexylene glycol . . . . . . . . . . . . . . . . . . .381382 Hexyl 2-ethylhexyl phthalate . . . . . . . . . . . .368 1-Hexyl mercaptan . . . . . . . . . . . . . . . . . . . . .434 Hexyl methacrylate . . . . . . . . . . . . . . . . . . . .410 Histidine (His) . . . . . . . . . . . . . . . . . . . . . . . . .604 HMX . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .624 Holotransferrin . . . . . . . . . . . . . . . . . . . . . . . .612 Homovanillic acid . . . . . . . . . . . . . . . . . . . . .645 Honey . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .637 Hormones, plant . . . . . . . . . . . . . . . . . . . . . .629 1,2,3,4,6,7,8-HpCDD . . . . . . . . . . . . . . . . . . . .360 1,2,3,4,6,7,9-HpCDD . . . . . . . . . . . . . . . . . . . .360 1,2,3,4,6,7,8-HpCDF . . . . . . . . . . . . . . . . . . . .360 1,2,3,4,7,8,9-HpCDF . . . . . . . . . . . . . . . . . . . .360 -Humulene . . . . . . . . . . . . . . . . . . . . . . . . . .384 Hydride gases . . . . . . . . . . . . . . . . . . . . . . . .414 Hydrocaffeic acid . . . . . . . . . . . . . . . . . . . . . .555 Hydrocarbons, halogenated . . . . . . . . . . . . .405 Hydrocodone . . . . . . . . . . . . . . . . . . . . . . . . .415 Hydrocortisone . . . . . . . . . . . . . . . . . . . . . . . .652 Hydrogen sulfide . . . . . . . . . .380, 425, 432434 Hydroxy acetate . . . . . . . . . . . . . . . . . . . . . . .395 4-Hydroxy benzaldehyde . . . . . . . . . . . . . . . .634 4-Hydroxy benzoic acid . . . . . . . . . . . . . . . . .634 4-Hydroxy-4-methyl-2-pentanone . . . . . . . . .394 Hydroxycitronellal . . . . . . . . . . . . . . . . .381382 Hydroxyindoleacetic acid . . . . . . . . . . . . . . .645 Hydroxyphenamate . . . . . . . . . . . . . . . . . . . .415 11 -hydroxyprogesterone . . . . . . . . . . . . . .649 17 -hydroxyprogesterone . . . . . . . . . . . . . .649 Hydroxypropionitrile . . . . . . . . . . . . . . . . . . .372 Hydroxypropyl acrylate . . . . . . . . . . . . . . . . .410 Hydroxytryptamine . . . . . . . . . . . . . . . . . . . . .645 Hydroxyzine . . . . . . . . . . . . . . . . . . . . . . . . . .417 Hypericin . . . . . . . . . . . . . . . . . . . . . . . . . . . .648 Hypnotics . . . . . . . . . . . . . . . . . . . . . . . . . . . .420 Imipramine . . . . . . . . . . . . . . . . . . . . . . .416, 605 Impramine . . . . . . . . . . . . . . . . . . . . . . . . . . .650 Indan . . . . . . . . . . . . . . . . . . . . . . . . . . . .438439 Indeno(1,2,3,-cd) pyrene . . . . .366367, 626627 3-Indole acetic acid . . . . . . . . . . . . . . . . . . . .629 3-Indole butyric acid . . . . . . . . . . . . . . . . . . .629 3-Indole proprionic acid . . . . . . . . . . . . . . . .629 Inosine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .607 Inositol . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .388 Insulin . . . . . . . . . . . . . . . . . . .533, 535, 611, 612 Iodobenzene . . . . . . . . . . . . . . . . . . . . . .399400 Iodoform . . . . . . . . . . . . . . . . . . . . . . . . . . . . .405 Iodoethane . . . . . . . . . . . . . . . . . . .399400, 413 Iodomethane . . . . . . . . . . . . . . . . .371, 373, 405 Iodononafluorobutane . . . . . . . . . . . . . . . . . .413 Iodopropane . . . . . . . . . . . . . . . . . . . . . . . . . .413 Isoamyl acetate . . . . . . . . . . . . . . . . . . .388, 402 Isoamyl alcohol . . . . . . . . . . . . . . . . . . . . . . .388 Isoamyl butyrate . . . . . . . . . . . . . . . . . . . . . .388 Isoamyl mercaptan . . . . . . . . . . . . . . . . . . . .434 Isoamyl salicylate . . . . . . . . . . . . . . . . .381, 383 Isoborneol . . . . . . . . . . . . . . . . . . .381383, 387 Isobutane . . . . . . . . . . . . . . . . . . . . . . . .426431 Isobutanol . . . . . . .372, 388, 394, 408, 410, 436 Isobutyl acetate . . . . . . .381382, 402, 407, 410 Isobutyl acrylate . . . . . . . . . . . . . . . . . . . . . .410 Isobutylbenzene . . . . . . . . . .399400, 407, 437 Isobutylene . . . . . . . . . . . . . . . . . . . . . .426429 Isobutyl mercaptan . . . . . . . . . . . . . . . . . . . .434 Isobutyl propionate . . . . . . . . . . . . . . . . . . . .410 Isobutyraldehyde . . . . . . . . . . . . . . . . . . . . . .398 Isobutyric acid . . . . . . . . . . . . . . . . . . . .390, 395 Isodrin . . . . . . . . . . . . . . . . . . . . . . . . . . .350, 355 trans-Isoeugenol . . . . . . . . . . . . . . . . . . . . . .383 Isoleucine (Ile) . . . . . . . . . . . . . . . . . . . . . . . .604 Isomaltose . . . . . . . . . . . . . . . . . . . . . . . . . . .635 Isomenthone . . . . . . . . . . . . . . . . .381382, 385 Isooctane (2,2,4-trimethylpentane) . . . . . . . . .348, 406407, 430, 437439 Isopentane . . . . . . . . . . . . . . .426431, 437438 Isophorone . . . . . . . . . . . . . . . . . . . . . . .366367 Isoprene . . . . . . . . . . . . . . . . . . . . . . . . .428430 Isopropanol . . . . . . . . . . . . . .394, 408, 422, 424 Isopropyl acetate . . . . . . . . . . . . . .402, 407408 Isopropyl acrylate . . . . . . . . . . . . . . . . . . . . .410 Isopropyl alcohol . . . . . . . . . .372, 379, 407, 423 4-Isopropyl aniline . . . . . . . . . . . . . . . . . . . . .622 Isopropyl benzene (cumene) . . . .371373, 375, 399401, 407, 430, 435437 Isopropyl ether . . . . . . . . . . . . . . . . . . . . . . . .403 Isopropyl mercaptan . . . . . . . . . . . . . . . . . . .434 Isopropyl myristate . . . . . . . . . . . . . . . .381382 2-Isopropylphenol . . . . . . . . . . . . . . . . . . . . .413 4-Isopropyltoluene . . . . . . . . . . . . .371, 373, 375 Isopropyltoluene . . . . . . . . . . . . . . . . . . . . . .372 Isoproturon . . . . . . . . . . . . . . . . . .625, 628629 Isopteropodin . . . . . . . . . . . . . . . . . . . . . . . . .649 Isorrhynchophyllin . . . . . . . . . . . . . . . . . . . . .649 Isovaleric acid . . . . . . . . . . . . . . . . . . . .390, 395
K
Karbutyl . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .628 Kelthane . . . . . . . . . . . . . . . . . . . . . . . . . . . . .350 Ketamine . . . . . . . . . . . . . . . . . . . . . . . . . . . .421 Ketimine . . . . . . . . . . . . . . . . . . . . . . . . . . . . .420 Ketones . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .398 Kinetin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .629 Krypton . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .425
L
Lactic Acid . . . . . . . . . . . . . . . . . . . . . . . . . . .621 Lactose . . . . . . . . . . . . . . . . . . . . . .592, 633, 637 Lactulose . . . . . . . . . . . . . . . . . . . . . . . . . . . .592 Lauric acid methyl ester (C12:0) . . . . . . . . . .392 Leptophos . . . . . . . . . . . . . . . . . . . . . . .356357 Leucine (Leu) . . . . . . . . . . . . . . . . . . . . . . . . .604 Leucine Enkephalin . . . . . . . . . . . . . . . .611612 Lidocaine . . . . . . . . . . . .416417, 421, 526, 643 Lidoflazine . . . . . . . . . . . . . . . . . . . . . . . . . . .557 Lignoceric acid methyl ester (C24:0) . . . . . .392 Limonen . . . . . . . . . . . . . . . . . . . . . . . . . . . . .385 Limonene . . . . . . . . . . . . . . . .381383, 385387 -Limonene . . . . . . . . . . . . . . . . . . . . . . . . . .384 R(+)Limonene . . . . . . . . . . . . . . . . . . . . . . . . .387 S(-)Limonene . . . . . . . . . . . . . . . . . . . . . . . . .387 Linalool . . . . . . . . . . . . . . . . . . . . . . . . . .381387 cis-Linalool oxide . . . . . . . . . . . . . . . . . .381382 trans-Linalool oxide . . . . . . . . . . . . . . . .381382 Linalyl acetate . . . . . . . . . . . . . . . . . . . .381383 Lindane . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .352 Linoleic acid methyl ester (C18:2n6c) . . . . .392 Linolelaidic acid methyl ester (C18:2n6t) . .392 Linolenic acid methyl ester (C18:3n3) . . . . .392 -Linolenic acid methyl ester (C18:3n6) . . . .392 Linuron . . . . . . . . . . . . . . . . . . . . . . . . . .625, 628 Lofentanyl . . . . . . . . . . . . . . . . . . . . . . . . . . . .419 Lorazepam . . . . . . . . . . . . . . . . . . . . . . . . . . .419 LSD . . . . . . . . . . . . . . . . . . . . . . . . .416, 420, 651 Lysine (Lys) . . . . . . . . . . . . . . . . . . . . . . . . . .604 Lysozyme . . . . . . . .533, 535, 611612, 615617
M
Malathion . . . . . . . . . . . . . . . . . . . . . . . .356357 Maleate . . . . . . . . . . . . . . . . . . . . . . . . .530, 545 Maleic acid . . . . . . . . . . . . . . . . . . . . . . . . . . .606 Maltose . . . . . . . . . . . . . . . . . . . . .592, 633, 637 Mannitol . . . . . . . . . . . . . . . . . . . . . . . . . . . . .388 D-Mannose . . . . . . . . . . . . . . . . . . . . . . . . . . .389 MCPA . . . . . . . . . . . . . . . . . . . . . . .357358, 629 MCPB . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .629 MCPP . . . . . . . . . . . . . . . . . . . . . . .357358, 629 Meclizine . . . . . . . . . . . . . . . . . . . . . . . . . . . .417 Medazepam . . . . . . . . . . . . . . . . . . . . . . . . . .419 Menthol . . . . . . . . . . . . . . . . . . . . . . . . .383, 385 1R,2S,5R-(-)-Menthol . . . . . . . . . . . . . . . . . . .383 1S,2R,5S-(+)-Menthol . . . . . . . . . . . . . . . . . .383 Menthone . . . . . . . . . . . . . . . . . . . . . . . .381382 Methanol . . . . . . . . . . . . . . . . . . . . . . . . . . . .431 Meperidine . . . . . . . . . . . . . . . . . . . . . . . . . . .416 Mephobarbital . . . . . . . . . . . . . . . . . . . . . . . .418 Mepivacaine . . . . . . . . . . . . . . . . . . . . . . . . . .417 Meprobamate . . . . . . . . . . . . . . . . . . . . .415, 420
Indices
I
Ibogaine . . . . . . . . . . . . . . . . . . . . . . . . .416, 420 Ibuprofen . . . . . . . . . . . . . . . . . . . . . . . .415, 421 IgG . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .586, 605 IgM . . . . . . . . . . . . . . . . . . . . . . . . .586, 605, 616
J
Jasmolin I . . . . . . . . . . . . . . . . . . . . . . . . . . . .364 Jasmolin II . . . . . . . . . . . . . . . . . . . . . . . . . . .364 cis-Jasmone . . . . . . . . . . . . . . . . . . . . . . . . . .386
714
Compound Index
Merphos . . . . . . . . . . . . . . . . . . . . .356357, 367 Mescaline . . . . . . . . . . . . . . . . . . . . . . . . . . . .420 Mesitylene . . . . . . . . . . . . . . . . . . . . . . .399, 406 Mesityl oxide . . . . . . . . . . . . . . . . . . . . . . . . .408 Mesterolone . . . . . . . . . . . . . . . . . . . . . . . . . .422 Metabromuron . . . . . . . . . . . . . . . . . . . . . . . .628 Metalacher . . . . . . . . . . . . . . . . . . . . . . . . . . .628 Metamitron . . . . . . . . . . . . . . . . . . . . . .628629 Metazachlor . . . . . . . . . . . . . . . . . . . . . .625, 628 Met-enkephalin . . . . . . . . . . . . . . . . . . .611612 Methabenzthiazuron . . . . . . . . . . . . . . .625, 628 Methacrolein . . . . . . . . . . . . . . . . . . . . .398, 409 Methacrolein-DNPH . . . . . . . . . . . . . . . . . . .622 Methacrylonitrile . . . . . . . . . . . . . .371372, 409 Methadone . . . . . . . . . . . . . . . . . . . . . . .416, 645 Methadone primary metabolite . . . . . . . . . .416 Methamphetamine . . . . . . . . . . . . . . . . . . . .416 Methane . . . .413, 425428, 430431, 433, 437 Methanol . . . . . . . .388, 394, 399, 406, 408, 410, 422424, 431, 436437 Methapyrilene . . . . . . . . . . . . . . . . . . . .415, 417 Methaqualone . . . . . . . . . . . . . . . . . . . .416, 420 Methaxytyrosine . . . . . . . . . . . . . . . . . . . . . .645 Methionine . . . . . . . . . . . . . . . . . . . . . . . . . . .604 Methofuran . . . . . . . . . . . . . . . . . . . . . . . . . .385 Methohexital . . . . . . . . . . . . . . . . . . . . . . . . .418 Methone . . . . . . . . . . . . . . . . . . . . . . . . .385386 Methoprene . . . . . . . . . . . . . . . . . . . . . . . . . .364 2-Methoxy-5-methylaniline . . . . . . . . . . . . . .411 3-Cl-4-methoxyaniline . . . . . . . . . . . . . . . . . .622 Methoxychlor . . . . . . . . . . . . . . . . .349355, 367 2-Methoxyethanol (methyl Cellosolve) . . . .394 bis(4-Methoxyethyl) phthalate . . . . . . . . . . .368 Methsuximide . . . . . . . . . . . . . . . . . . . . . . . .417 Methyl acetate . . . . . . . . . . . . . . . .385, 402, 410 Methyl acetone . . . . . . . . . . . . . . . . . . . . . . .433 Methyl acetylene . . . . . . . . . . . . . . . . . . . . . .433 Methyl acrylate . . . . . . . . . . . . . . .371372, 410 Methylamine . . . . . . . . . . . . . . . . . . . . . . . . .396 4-Methylaminorex . . . . . . . . . . . . . . . . . . . . .420 3-Cl-4-methylaniline . . . . . . . . . . . . . . . . . . .622 -Methylansiole . . . . . . . . . . . . . . . . . . . . . . .386 2-Methylanthracene . . . . . . . . . . . . . . . . . . .368 9-Methylanthracene . . . . . . . . . . . . . . . . . . .368 Methyl arachidate (C20:0) . . . . . . . . . . .390, 393 Methyl benzoate . . . . . . . . . .381382, 386, 402 N-methylbenzylamine . . . . . . . . . . . . . . . . . .546 2-Methyl butanal . . . . . . . . . . . . . . . . . . . . . .397 2-Methyl-1-butanol (amyl alcohol) . . . .388, 394 3-Methyl-1-butanol (isoamyl alcohol) . . . .388, 394 2-Methyl-2-butanol (tert-amyl alcohol) . . . .394 3-Methyl-2-butanone . . . . . . . . . . . . . . . . . . .398 2-Methyl-3-buten-2-ol . . . . . . . . . . . . . . . . . .394 2-Methyl-1-butene . . . . . . . . . . . . . . . . . . . . .438 3-Methyl-1-butene . . . . . . . . . . . . . . . . .428430 2-Methyl-2-butene . . . . . . . . . . . . .426, 430, 438 3-Methyl-2-buten-1-ol . . . . . . . . . . . . . . . . . .394 trans-2-Methyl-2-butenoic acid . . . . . . . . . .395 2-Methylbutyl acetate . . . . . . . . . . . . . . . . . .402 2-Methylbutyl alcohol . . . . . . . . . . . . . .381382 3-Methylbutyl alcohol . . . . . . . . . . . . . .381382 Methyl tert-butyl ether (MTBE) . . . .347, 371-372, 403, 406, 423, 436, 439 Methyl butyrate . . . . . . . . . . . . . . . . . . .381382 2-Methyl butyric acid . . . . . . . . . . . . . . . . . . .395 3-Methylcholanthrene . . . . . . . . . . . . . . . . . .368 Methyl-cresol . . . . . . . . . . . . . . . . . . . . .381-382 Methylcyclohexane . . . . . . . . . . . . . . . . . . . .430 Methylcyclopentane . . . . . . . . . . .430, 437438 1-Methyl-1-cyclopentene . . . . . . . . . . . . . . .438 Methyl decanoate . . . . . . . . . . . . . . . . . . . . .402 Methyl-2,4-dichlorophenylacetate . . . . . . . .357 4-Methyl-2,5-dimethoxyamphetamine (STP) . . .416 2-Methyl-4,6-dinitrophenol . . . . . .369, 412, 629 Methyl disulfide . . . . . . . . . . . . . . . . . . .384, 432 cis-1-(Methyldithio)-1-propene . . . . . . . . . . . .384 trans-1-(Methyldithio)-1-propene . . . . . . . . . .384 Methyl dodecanoate . . . . . . . . . . . . . . . . . . .402 Methyl eicosenoate . . . . . . . . . . . . . . . .393, 402 Methyl elaidate . . . . . . . . . . . . . . . . . . . . . . .390 trans-Methyl elaidate (C18:1) . . . . . . . . . . . .393 Methylene chloride . . . . . . . . . . .371373, 375, 378379, 405, 407408, 423424 4,4'-Methylenedianiline . . . . . . . . . . . . . . . .411 3,4-Methylenedioxyamphetamine (MDA) . . . .416 3,4-Methylenedioxyethylamphetamine (MDE; Eve) . . . . . . . . . . . . . . . . . . . . . . . . .416 3,4-Methylenedioxymethylamphetamine . . . .416 Methyl ephedrine . . . . . . . . . . . . . . . . . . . . . .416 1-Methyl-2-ethylbenzene . . . . . . . . . . . . . . .439 1-Methyl-3-ethylbenzene . . . . . . . . . . . . . . .439 1-Methyl-4-ethylbenzene . . . . . . . . . . . . . . .439 Methyl ethyl ketone (MEK) . . . . . . . . .379, 398, 407408, 423424 2-Methylfluoranthene . . . . . . . . . . . . . . . . . .368 Methyl formate . . . . . . . . . . . . . . . . . . .402, 408 Methyl heptadecanoate . . . . . . . . . . . . . . . .390 2-Methylheptane . . . . . . . . . . . . . .430, 437438 3-Methylheptane . . . . . . . . . . . . . . . . . .430, 438 4-Methylheptane . . . . . . . . . . . . . . . . . . . . . .437 Methyl hexadecanoate . . . . . . . . . . . . . . . . .402 2-Methylhexane . . . . . . . . . . . . . . . . . . . . . . .430 3-Methylhexane . . . . . . . . . . . . . . . . . . . . . . .430 Methyl cis-9,10-methyl hexadecanoate . . . .390 Methyl 2-hydroxydecanoate . . . . . . . . . . . . .390 Methyl 2-hydroxydodecanoate . . . . . . . . . . .390 Methyl 3-hydroxydodecanoate . . . . . . . . . . .390 Methyl 2-hydroxyhexadecanoate . . . . . . . . .390 Methyl 2-hydroxytetradecanoate . . . . . . . . .390 Methyl 3-hydroxytetradecanoate . . . . . . . . .390 -Methyl Ionone . . . . . . . . . . . . . . . . . . . . . .383 Methyl isobutyl ketone . . . . . . . . . . . . .407408 Methyl laurate . . . . . . . . . . . . . . . . . . . . . . . .390 Methyl linolenate (C18:3) . . . . . . . . . . . . . . .393 Methyl mercaptan . . . . . . . . . . . . .380, 432434 Methyl methacrylate . . . . . . . . . . .371372, 410 Methyl 12-methyltetradecanoate . . . . . . . . .390 Methyl 14-methylhexadecanoate . . . . . . . . .390 Methyl myristate . . . . . . . . . . . . . . . . . . . . . .390 1-Methylnaphthalene . . . . . . . . . . . . . .368, 439 2-Methylnaphthalene . . . . . .366, 368, 438439 2-Methyl-5-nitroaniline . . . . . . . . . . . . . . . . .411 Methyl nonadecanoate . . . . . . . . . . . . . . . . .390 Methyl octadecanoate . . . . . . . . . . . . . . . . .402 Methyl oleate . . . . . . . . . . . . . . . . . . . . . . . . .390 cis-Methyl oleate (C18:1) . . . . . . . . . . . . . . .393 Methyl palmitate (C16:0) . . . . . . . . . . . .390, 393 Methyl palmitoleate (C16:1) . . . . . . . . .390, 393 Methyl paraben . . . . . . . . . . . . . . . . . . . . . . .638 Methyl paraoxon . . . . . . . . . . . . . . . . . . . . . .367 Methyl parathion . . . . . . . . . . . . . . . . . .356357 Methyl pentadecanoate . . . . . . . . . . . . . . . .390 2-Methylpentane . . . . . . . . . . . . . .347, 430, 438 3-Methylpentane . . . . . .347348, 406, 430, 438 3-Methyl-3-pentanol . . . . . . . . . . . . . . . . . . .395 4-Methyl-2-pentanol . . . . . . . . . . . . . . .394395 2-Methyl-3-pentanone . . . . . . . . . . . . . . . . . .398 4-Methyl-2-pentanone . . . . . . . . . . . . . . . . . .371 4-Methyl-2-pentanone (MIBK) . . . . . . . . . . .379 2-Methyl-1-pentene . . . . . . . . . . . . . . . .430, 438 4-Methyl-1-pentene . . . . . . . . . . . . . . . . . . . .430 2-Methylphenol . . . . . . . . . . . . . . . . . . .366, 369 3-Methylphenol . . . . . . . . . . . . . . . . . . . . . . .369 4-Methylphenol . . . . . . . . . . . . . . . . . . .366, 369 -Methylphenyl alcohol . . . . . . . . . . . . . . . .394 5-Methyl-5-phenylhydantoin . . . . . . . . . . . . .417 bis(4-Methyl-2-pentyl) phthalate . . . . . . . . .368 2-Methyl-1-propanethiol . . . . . . . . . . . .432, 434 1-Methyl-1-propnaethiol . . . . . . . . . . . . . . . .434 Methyl propionate . . . . . . . . . . . . . . . . .402, 410 1-Methyl-3-propylbenzene . . . . . . . . . . . . . .438 Methylpropyldisulfide . . . . . . . . . . . . . .384, 389 1-Methyl-1-propyl mercaptan . . . . . . . . . . . .434 2-Methyl-1propyl mercaptan . . . . . . . . . . . .434 2-Methyl-2-propyl mercaptan . . . . . . . . . . . .434 Methylpropyltrisulfide . . . . . . . . . . . . . . . . . .384 1-Methyl-2-pyrrolidinone . . . . . . . . . . .397, 409 Methyl salicylate . . . . . . . . . . . . . . . . . . . . . .633 Methyl stearate (C18:0) . . . . . . . . . . . . .390, 393 4-Methylstyrene . . . . . . . . . . . . . . . . . .399400 -Methylstyrene . . . . . . . . . .399401, 435-436 -Methylstyrene . . . . . . . . . . . . . . . . . . . . . .436 17-Methyltestosterone . . . . . . . . . . . . . . . .422 Methyl tetradecanoate . . . . . . . . . . . . . . . . .402 Methyl thiophenes . . . . . . . . . . . . . . . .432, 434 Methyl tridecanoate . . . . . . . . . . . . . . . . . . .390 Methtyl tripentyltin . . . . . . . . . . . . . . . . . . . .365 Methylundecanoate . . . . . . . . . . . . . . . . . . .390 Metobromuron . . . . . . . . . . . . . . . . . . . .625, 628 Metolachlor . . . . . .351, 356, 358, 367, 624625 Metolachor . . . . . . . . . . . . . . . . . . . . . . . . . . .596 Metoxuron . . . . . . . . . . . . . . . . . . . . . . .625, 628 Metprolol . . . . . . . . . . . . . . . . . . . . . . . . . . . .605 Metribuzin . . . . . . . . . . .351, 356, 358, 367, 628 Metronidazole . . . . . . . . . . . . . . . . . . . . . . . .648 Metsulfuron-methyl . . . . . . . . . . . . . . . . . . . .630 Mevinphos . . . . . . . . . . . . . . . . . . .356357, 367 MGK-264 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .356 MGK-264 (isomer A) . . . . . . . . . . . . . . . . . . .367 MGK-264 (isomer B) . . . . . . . . . . . . . . . . . . .367 Miconazole . . . . . . . . . . . . . . . . . . . . . . . . . . .644 Milk (2%) . . . . . . . . . . . . . . . . . . . . . . . . . . . .637 Milk, Lactose-free . . . . . . . . . . . . . . . . . . . . .637 Mirex . . . . . . . . . . . . . . . . . . . . . . . . . . . .350, 355 Mitraphyllin . . . . . . . . . . . . . . . . . . . . . . . . . .649 Molasses . . . . . . . . . . . . . . . . . . . . . . . . . . . .637 Molinate . . . . . . . . . . . . . . . . . . . . .356, 358, 367 Monlinuron . . . . . . . . . . . . . . . . . . . . . . . . . . .625 6-Monoacetylmorphine . . . . . . . . . . . . . . . . .420 Monocrotophos . . . . . . . . . . . . . . . . . . .356357 Monolinuron . . . . . . . . . . . . . . . . . . . . . . . . . .628 Monuron . . . . . . . . . . . . . . . . . . . . . . . . . . . . .358 Morphine . . . . . . . . . . . . . . . .415, 420421, 648 Morphine-3-glucuronide . . . . . . . . . . . . . . . .648 Morphine-6-glucuronide . . . . . . . . . . . . . . . .648 MTBE . . . . . . . . . . . . . . . . . . . . . . .372, 423, 436 Musk ketone . . . . . . . . . . . . . . . . . . . . . . . . .383
Indices
715
Compound Index
Musk T (ethylene brassylate) . . . . . . . .381382 Musk xylene . . . . . . . . . . . . . . . . . . . . . . . . . .383 Myoglobin . . . . . . .533, 535, 611612, 614, 616 Myrcene . . . . . . . . . . . . . . . . . . . . . . . . .381386 Myristic acid methyl ester (C14:0) . . . . . . . .392 Myristoleic acid methyl ester (C14:1) . . . . .392 2-Nitrotoluene . . . . . . . .399400, 407, 623624 3-Nitrotoluene . . . . . . . .399400, 407, 623624 4-Nitrotoluene . . . . . . . .399400, 407, 623624 2-Nitroxylene . . . . . . . . . . . . . . . . . . . . . . . . .359 cis-Nonachlor . . . . . . . . . . . . . . . . . . . . . . . . .352 trans-Nonachlor . . . . . . . . . . . . . . .350, 352, 367 Nonadec-1-ene . . . . . . . . . . . . . . . . . . .381382 Nonadecane . . . . . . . . . . . . . . . . . . . . . .381382 -Nonalactone . . . . . . . . . . . . . . . . . . . .381382 Nonanal . . . . . . . . . . . . . . . . . . . . . . . . .384385 n-Nonane . . . . . . . . . . . . . . . . . . . .430, 437439 Nonane . . . . . . . . . . . . . . . . . . . . . . . . . .406, 435 1,9-Nonanediol . . . . . . . . . . . . . . . . . . .403404 1-Nonanol . . . . . . . . . . . . . . . . . . . . . . . . . . . .394 Nonanol . . . . . . . . . . . . . . . . . . . . . . . . . . . . .387 Nonaromatics . . . . . . . . . . . . . . . . . . . . . . . . .436 Nonyl aldehyde . . . . . . . . . . . . . . . . . . . . . . .398 n-Nonylamine . . . . . . . . . . . . . . . . . . . .396397 Nootkatone . . . . . . . . . . . . . . . . . . . . . . . . . .385 Norcodeine . . . . . . . . . . . . . . . . . . . . . . . . . . .420 Norepinephrine . . . . . . . . . . . . . . . . . . . . . . .645 Norethandrolone . . . . . . . . . . . . . . . . . . . . . .422 Norfluoxetine . . . . . . . . . . . . . . . . . . . . . . . . .646 Norflurazon . . . . . . . . . . . . . . . . . .356, 358, 367 Norgestral . . . . . . . . . . . . . . . . . . . . . . . . . . . .645 Normorphine . . . . . . . . . . . . . . . . . . . . . . . . .420 19-Nortestosterone (Nandrolone) . . . . . . . .422 19-Nortestosterone-17-decanoate . . . . . . . .422 Nortriptyline . . . . . . . . . . . . . . . . . .605606, 650 cis 10-OH- Nortriptyline . . . . . . . . . . . . . . . .650 trans 10-OH- Nortriptyline . . . . . . . . . . . . . . .650 Norvaline (Nva) . . . . . . . . . . . . . . . . . . . . . . . .604 Nucleosides . . . . . . . . . . . . . . . . . . . . . . . . .607 Nucleotides . . . . . . . . . . . . . . . . . . . . . . . . . .608 Oil, Rosemary . . . . . . . . . . . . . . . . . . . . . . . . .387 Oil, Spearmint . . . . . . . . . . . . . . . . . . . . . . . . .386 Oil, Sunflower . . . . . . . . . . . . . . . . . . . . . . . . .635 Oil, Ylang Ylang . . . . . . . . . . . . . . . . . . . . . . .386 Oils, essential . . . . . . . . . . . . . . . . . . . . . . . . .383 Oleic acid methyl ester (C18:1n9c) . . . . . . .392 Oligonucleotides . . . . . . . . . . . . . .594, 608609 Opiates (in urine) . . . . . . . . . . . . . . . . . . . . . .130 Orange juice . . . . . . . . . . . . . . . . . . . . . . . . . .633 Organomercury Compounds . . . . . . . . . . . . .365 Organotin Compounds . . . . . . . . . . . . . . . . . .365 Orotic Acid . . . . . . . . . . . . . . . . . . . . . . . . . . .608 Ovalbumin . . . . . . . . . . . . . . .535, 612, 615617 Oxazepam . . . . . . . . . . . . . . . . . . . . . . . . . . . .419 Oxindole Alkaloids . . . . . . . . . . . . . . . . . . . . .649 Oxprenolol . . . . . . . . . . . . . . . . . . . . . . . . . . .605 4,4'-Oxydianiline . . . . . . . . . . . . . . . . . . . . . .411 Oxygen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .425 Oxygenates . . . . . . . . . . . . . . . . . . . . . . . . . . .436 Oxymetholone . . . . . . . . . . . . . . . . . . . . . . . .422 Oxymorphone . . . . . . . . . . . . . . . . . . . . .415, 648 Oxytetracycline . . . . . . . . . . . . . . . . . . .649650
N
Naled . . . . . . . . . . . . . . . . . . . . . . . . . . .356357 Naphthalene . . . . . . . . . . . . .347348, 366, 368, 371373, 406, 437439, 626 Naphthalene-d8 . . . . . . . . . . . . . . . . . . .366, 439 1-Naphthol . . . . . . . . . . . . . . . . . . . . . . . . . . .412 1-Naphthyl acetamide . . . . . . . . . . . . . . . . . .629 1-Naphthyl acetic acid . . . . . . . . . . . . . . . . .629 Napropamide . . . . . . . . . . . . . . . . .356, 358, 367 Napthalene . . . . . . . . . . . . . . . . . . .375, 537, 627 Narcotics . . . . . . . . . . . . . . . . . . . . . . . . . . . .421 Naringenine . . . . . . . . . . . . . . . . . . . . . . . . . .633 NC4 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .431 NC5 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .431 N-Desmethyl methsuximide . . . . . . . . . . . . .417 Nefopam . . . . . . . . . . . . . . . . . . . . . . . . . . . . .417 Neomenthol . . . . . . . . . . . . . . . . . . . . . .383, 385 Neon . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .425 Neorotensin . . . . . . . . . . . . . . . . . . . . . . . . . .535 Neral . . . . . . . . . . . . . . . . . . . .381382, 384385 cis-Nerolidol . . . . . . . . . . . . . . . . . . . . . . . . . .381 trans-Nerolidol . . . . . . . . . . . . . . . . . . . . . . . .381 Nervonic acid methyl ester (C24:1) . . . . . . .392 Neryl acetate . . . . . . . . . . . . . . . . .381382, 384 Neurotensin . . . . . . . . . . . . . . . . . . . . . .611612 Niacin (Vitamin B3) . . . . . . . . . . . . . . . .641642 Niacinamide . . . . . . . . . . . . . . . . . . . . . .531, 642 Nicotinamine . . . . . . . . . . . . . . . . . . . . . . . . .416 Nicotine . . . . . . . . . . . . . . . . . . . . .415416, 421 Nicotinic Acid . . . . . . . . . . . . . . . . . . . .531, 642 Nifedipine . . . . . . . . . . . . . . . . . . . . . . . . . . . .557 2,4-Nimethylphenol . . . . . . . . . . . . . . . . . . . .629 2-Nitroaniline . . . . . . . . . . . . . . . . .366, 397, 411 3-Nitroaniline . . . . . . . . . . . . . . . . .366, 397, 411 4-Nitroaniline . . . . . . . . . . . . . . . . .366, 397, 411 Nitrobenzene . . . . . . . . .366, 371372, 399400 Nitrobenzene-d5 . . . . . . . . . . . . . . . . . . . . . .366 2-Nitrobenzoic acid . . . . . . . . . . . . . . . . . . . .644 3-Nitrobenzoic acid . . . . . . . . . . . . . . . . . . . .644 2-Nitrobiphenyl . . . . . . . . . . . . . . . . . . . . . . .368 3-Nitrobiphenyl . . . . . . . . . . . . . . . . . . . . . . .368 4-Nitrobiphenyl . . . . . . . . . . . . . . . . . . . . . . .368 Nitrodiglycol . . . . . . . . . . . . . . . . . . . . . . . . . .623 Nitrogen . . . . . . . . . . . . . . . . . . . . . . . . .414, 425 Nitroglycerine . . . . . . . . . . . . . . . . . . . . . . . .623 Nitroglycol . . . . . . . . . . . . . . . . . . . . . . . . . . .623 1-Nitronaphthalene . . . . . . . . . . . . . . . . . . . .368 2-Nitronaphthalene . . . . . . . . . . . . . . . . . . . .368 Nitropenta . . . . . . . . . . . . . . . . . . . . . . . . . . .623 2-Nitrophenol . . . . . . . . . . . . .366, 369, 412, 629 3-Nitrophenol . . . . . . . . . . . . . . . . . . . . . . . . .369 4-Nitrophenol . . . . . . . . . . . . .357, 366, 369, 412 2-Nitropropane . . . . . . . . . . . . . . . . . . . .371372 Nitropropane . . . . . . . . . . . . . . . . . . . . . . . . .408 n-Nitrosodimethylamine . . . . . . . . . . . . . . . .366 n-Nitroso-di-n-propylamine . . . . . . . . . . . . . .366 n-Nitrosodiphenylamine . . . . . . . . . . . . . . . .366
P
PAHs . . . . . . . . . . . . . . . . . . . . . . . .368, 626, 627 Palatinose . . . . . . . . . . . . . . . . . . . . . . . . . . . .635 Palmitic acid methyl ester (C16:0) . . . . . . . .392 Palmitoleic acid methyl ester (C16:1) . . . . .392 Pancake Syrup . . . . . . . . . . . . . . . . . . . . . . . .637 Pantothenic acid . . . . . . . . . . . . . . . . . . . . . .641 Parabens . . . . . . . . . . . . . . . . . . . . . . . . . . . . .638 Paraffin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .441 Paraldehyde . . . . . . . . . . . . . . . . . . . . . . . . . .372 Paraquat . . . . . . . . . . . . . . . . . . . . . . . . . . . . .627 Parvalbumin . . . . . . . . . . . . . . . . . . . . . . . . . .533 Patuline . . . . . . . . . . . . . . . . . . . . . . . . . . . . .639 PCBs . . . . . . . . . . . . . . . . . . . . . . . . . . . .361363 PCB congeners . . . . . . . . . . . . . . . . . . .362, 363 PCP . . . . . . . . . . . . . . . . . . . . . . . . . . . . .416, 420 Pebulate . . . . . . . . . . . . . . . . . . . . . . . . .356, 358 1,2,4,6,8-/1,2,4,7,9-PeCDD . . . . . . . . . . . . . .360 1,2,4,8,9-PeCDD . . . . . . . . . . . . . . . . . . . . . . .360 1,2,3,4,6-PeCDF . . . . . . . . . . . . . . . . . . . . . . .360 1,2,3,4,8-PeCDF . . . . . . . . . . . . . . . . . . . . . . .360 1,2,3,7,8-PeCDF . . . . . . . . . . . . . . . . . . . . . . .360 1,2,3,8,9-PeCDF . . . . . . . . . . . . . . . . . . . . . . .360 1,3,4,6,8-PeCDF . . . . . . . . . . . . . . . . . . . . . . .360 2,3,4,7,8-PeCDF . . . . . . . . . . . . . . . . . . . . . . .360 PEMA . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .418 Pencycuron . . . . . . . . . . . . . . . . . . . . . . . . . .628 Pendimenthaline . . . . . . . . . . . . . . . . . . . . . .628 Pentaborane . . . . . . . . . . . . . . . . . . . . . . . . . .414 2,2',3',4,6-Pentachlorobiphenyl . . . . . . . . . .367 Pentachlorobenzene . . . . . . . . . . . . . . .399400 Pentachloroethane . . . . . . . . . . . .371372, 405 Pentachloronitrobenzene . . . . . . .350351, 355 Pentachlorophenol . . . . . . . .357358, 366367, 369, 412, 629 n-Pentadecane . . . . . . . . . . . . . . . . . . . . . . . .439 Pentadecanoic acid methyl ester (C15:0) . . . .392 cis-10-Pentadecenoic acid . . . . . . . . . . . . . . . . . methyl ester (C15:1) . . . . . . . . . . . . . . . . . . .392 cis-1,3-Pentadiene . . . . . . . . . . . . . . . . .428429 trans-1,3-Pentadiene . . . . . . . . . . . . . . .428429
O
OcCDD . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .360 OcCDF . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .360 Ochratoxin . . . . . . . . . . . . . . . . . . . . . . . . . . .639 cis-Ocimene . . . . . . . . . . . . . . . . . . . . . .385386 2,2',3,3',4,5',6,6'-Octachlorobinphenyl . . . . .367 n-Octacosane (n-C28) . . . . . . . . . . . . . .348, 439 Octadecane . . . . . . . . . . . . . . . . . . . . . . . . . .364 n-Octadecane (n-C18) . . . . . . . . . . . . . .348, 439 Octadecanoic acid . . . . . . . . . . . . . . . . . . . . .390 Octanal . . . . . . . . . . . . . . . . . . . . . .384385, 397 Octane . . . . . . . . . . . . . . . . . . . . . . . . . .406, 435 n-Octane . . . . . . . . . . . . . . . . . . . . .430, 437439 1,8-Octanediol . . . . . . . . . . . . . . . . . . . .403404 Octanoic acid . . . . . . . . . . . . . . . . . . . . .390, 397 1-Octanol . . . . . . . . . . . . . . . . . . . . . . . . . . . .394 3-Octanol . . . . . . . . . . . . . . . . . . . . . . . .385386 Octanol . . . . . . . . . . . . . . . . . .381382, 384, 387 1-Octen-3-ol . . . . . . . . . . . . . . . . . . . . . . . . . .385 Octogen (HMX) . . . . . . . . . . . . . . . . . . .623624 Octyl acetate . . . . . . . . . . . . . . . . .381382, 386 Octyl aldehyde . . . . . . . . . . . . . . . . . . . . . . . .398 n-Octylamine . . . . . . . . . . . . . . . . . . . . .396397 n-Octyl mercaptan . . . . . . . . . . . . . . . . . . . . .434 Octylphenoxy (polyethylene oxy) Ethanol . . . . . . .574 Oil, Garlic . . . . . . . . . . . . . . . . . . . . . . . . . . . .384 Oil, Lemon . . . . . . . . . . . . . . . . . . . . . . . . . . .384 Oil, Orange . . . . . . . . . . . . . . . . . . . . . . . . . . .385 Oil, Peppermint . . . . . . . . . . . . . . . . . . . . . . .385
Indices
716
Compound Index
Pentafluorobenzene . . . . . . . . . . . . . . .371, 372 Pentafluoroethane . . . . . . . . . . . . . . . . . . . . .380 Pentamethylbenzene . . . . . . . . . . . . . . . . . . .439 Pentanal . . . . . . . . . . . . . . . . . . . . . . . . . . . . .397 Pentane . . . . . . . . . . . . . . . . . . . . .402, 405, 436 iso-Pentane . . . . . . . . . . . . . . . . . .425, 431, 433 n-Pentane . . . . . . . . . . .425431, 433, 437439 neo-Pentane . . . . . . . . . . . . . . . . . . . . . . . . . .425 1,5-Pentanediol . . . . . . . . . . . . . . . . . . .403404 2,3-Pentanedione (acetyl propionyl) . . . .381382 1-Pentanethiol . . . . . . . . . . . . . . . . . . . . . . . .432 1-Pentanol . . . . . . . . . . . . . . . . . . . . . . .394395 2-Pentanol . . . . . . . . . . . . . . . . . . . . . . . . . . .394 3-Pentanol . . . . . . . . . . . . . . . . . . . . . . . . . . .394 2-Pentanone . . . . . . . . . . . . . . . . . .372, 398, 407 3-Pentanone . . . . . . . . . . . . . . . . . . . . . . . . . .398 1-Pentene . . . . . . . . . . . . . . . . . . . .426430, 438 cis-2-Pentene . . . . . . . . . . . . . . . . .426, 428430 trans-2-Pentene . . . . . . . . . . . . . . .426, 428430 4-Pentenoic acid . . . . . . . . . . . . . . . . . . . . . .395 trans-2-Pentenoic acid . . . . . . . . . . . . . . . . .395 trans-3-Pentenoic acid . . . . . . . . . . . . . . . . .395 1-Penten-3-ol . . . . . . . . . . . . . . . . . . . . . . . . .394 2-Penten-1-ol . . . . . . . . . . . . . . . . . . . . . . . . .394 Pentobarbital . . . . . . . . . . . . . . . . . . . . .415, 418 Pentyl ether . . . . . . . . . . . . . . . . . . . . . . . . . .403 1-Pentyl mercaptan . . . . . . . . . . . . . . . . . . . .434 Peptides . . . . . . . . . . . . . . . . . . . . . . . . .609-613 cis-Permethrin . . . . . . . . . . . .350352, 355, 367 trans-Permethrin . . . . . . . . . .350352, 355, 367 Peroxidase . . . . . . . . . . . . . . . . . . . . . . . . . . .614 Perthane . . . . . . . . . . . . . . . . . . . . . . . . . . . . .350 Perylene . . . . . . . . . . . . . . . . . . . . . . . . . . . . .368 Perylene-d12 . . . . . . . . . . . . . . . . . . . . .366367 Pesticides . . .349357, 625, 627629, 630, 640 PHB-ethyl . . . . . . . . . . . . . . . . . . . . . . . .632, 640 PHB-methyl . . . . . . . . . . . . . . . . . . . . . .632, 640 PHB-propyl . . . . . . . . . . . . . . . . . . . . . . .632, 640 -Phellandrene . . . . . . . . . . . . . . . . . . . . . . .384 Phenacetin . . . . . . . . . . . . . . . . . . .415416, 644 Phenacetin (acetophenetidin) . . . . . . . . .604, 643 Phenanthrene . . . . . . . . . . . .366368, 626627 Phenanthrene-d10 . . . . . . . . . . . . . . . . .366, 367 Phencyclidine (PCP) . . . . . . . . . . . . . . . . . . .416 Phenethylalcohol . . . . . . . . . . . . . . . . . . . . . .387 Pheniramine . . . . . . . . . . . . . . . . . . . . . . . . . .417 Phenmetrazine . . . . . . . . . . . . . . . . . . . . . . . .415 Phenobarbital . . . . . . . . . . . . . . . . . . . . .415418 Phenols . . . . . . . . . . . . .366, 369, 412413, 629 Phenol-d5 . . . . . . . . . . . . . . . . . . . . . . . . . . . .366 Phenoxycarboxylic Acids . . . . . . . . . . . . . . .629 2-Phenoxyethanol . . . . . . . . . . . . . . . . . . . . .394 Phensuximide . . . . . . . . . . . . . . . . . . . . . . . . .417 Phentermine . . . . . . . . . . . . . . . . . . . . . .416, 604 Phenylacetone . . . . . . . . . . . . . . . . . . . . . . . .416 Phenylacetylene . . . . . . . . . . . . . . . . . . .435-436 Phenylalanine (Phe) . . .527, 530, 555, 604, 632 9-Phenylanthracene . . . . . . . . . . . . . . . . . . .368 Phenylbutazone . . . . . . . . . . . . . . . . . . . . . . .644 L-phenyl-1-butene . . . . . . . . . . . . . . . . . . . . .402 Phenylephedrine . . . . . . . . . . . . . . . . . . . . . .416 Phenylephrine . . . . . . . . . . . . . . . . . . . . . . . .590 Phenylethyl acetate . . . . . . . . . . . . . . . . . . . .381 Phenylethylamine . . . . . . . . . . . . . . . . . . . . .397 Phenyl ethyl cinnamate . . . . . . . . . . . .381382 Phenylethyl tiglate . . . . . . . . . . . . . . . . .381382 Phenylethylmalonamide . . . . . . . . . . . . . . . .417 1-phenylhexane . . . . . . . . . . . . . . . . . . . . . . .402 2-phenyl-1-hexene . . . . . . . . . . . . . . . . . . . . .402 2-phenyl-2-hexene . . . . . . . . . . . . . . . . . . . . .402 2-Phenylnaphthalene . . . . . . . . . . . . . . . . . .368 Phenylpropanolamine . . . . . . . . . . . . . . . . . .530 Phenyltoin . . . . . . . . . . . . . . . . . . . . . . . . . . .418 Phenyltoloxamine . . . . . . . . . . . . . . . . . . . . .417 Phenytoin . . . . . . . . . . . . . . . . . . . . . . . . . . . .417 Phorate . . . . . . . . . . . . . . . . . . . . . . . . . .356357 Phosmet . . . . . . . . . . . . . . . . . . . . . . . . .356357 Phosphamidon . . . . . . . . . . . . . . . . . . . .356357 Phosphine . . . . . . . . . . . . . . . . . . . . . . . . . . . .414 Phthalate Esters . . . . . . . . . . . . . . . . . . . . . .368 Phthalic acid . . . . . . . . . . . . . . . . . . . . . . . . . .644 Picloram . . . . . . . . . . . . . . . . . . . . . . . . .357358 Picric acid . . . . . . . . . . . . . . . . . . . . . . . . . . . .624 Pindolol . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .605 -Pinene . . . . . . . . . . . . . . . .381382, 385387 -Pinene . . . . . . . . . . . . . . . . . . . . . . . . .381387 1S-(-)--pinene . . . . . . . . . . . . . . . . . . . . . . . .383 Piperazine . . . . . . . . . . . . . . . . . . . . . . . . . . . .397 5-benzyl-3, 6-dioxo-2-piperazineacetic acid . . .632 Piperitone . . . . . . . . . . . . . . . . . . . . . . . . . . . .385 Pirenzepine . . . . . . . . . . . . . . . . . . . . . . . . . . .651 PMMA . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .620 Polybutadiene . . . . . . . . . . . . . . . . . . . . . . . .619 Polychlorobiphenyl . . . . . . . . . . . . . . . . . . . .363 Polyethoxylate . . . . . . . . . . . . . . . . . . . . . . . .620 Polyethylene . . . . . . . . . . . . . . . . . . . . . .441, 619 Polyethyleneamines . . . . . . . . . . . . . . . . . . .396 Polyethylene glycol 200 . . . . . . . . . . . . . . . .404 Polyethylene glycol 400 . . . . . . . . . . . . . . . .404 Polyethylene glycol 600 . . . . . . . . . . . . . . . .404 Polymethacrylate . . . . . . . . . . . . . . . . . . . . . .619 Polymethoxyflavones . . . . . . . . . . . . . . . . . .385 Polysaccharides . . . . . . . . . . . . . . . . . . . . . . .635 Polystyrene . . . . . . . . . . . . . . . . . .583584, 619 Polyurethane . . . . . . . . . . . . . . . . . . . . . . . . .620 Polyurethane Resins . . . . . . . . . . . . . . . . . . .581 Polyvinyl Alcohol . . . . . . . . . . . . . . . . . . . . . .621 Ponceau 4R . . . . . . . . . . . . . . . . . . . . . .634, 638 Prazepam . . . . . . . . . . . . . . . . . . . . . . . . . . . .419 Prebane (Terbutryne) . . . . . . . . . . . . . . . . . . . .367 Prednisolone . . . . . . . . . . . . . . . . . . . . . . . . .649 Primidone . . . . . . . . . . . . . . . . . . . . . . . .417418 Procainamide . . . . . . . . . . . . . . . . . . . . .546, 649 Procaine . . . . . . . . . . . . .415, 417, 421, 526, 643 Progesterone . . . . . . . . . . . . . . . . .421, 649, 651 Proline (Pro) . . . . . . . . . . . . . . . . . . . . . . . . . .604 Promaton . . . . . . . . . . . . . . . . . . . . . . . . . . . .630 Promazine . . . . . . . . . . . . . . . . . . . . . . . . . . . .418 Promethazine . . . . . . . . . . . . . . . . . . . . .417418 Prometon . . . . . . . .356, 358359, 367, 596, 625 Prometron . . . . . . . . . . . . . . . . . . . . . . . . . . . .624 Prometryn . . . . . . . . . . . . . . . .356, 625, 628, 630 Prometryne . .358359, 367, 596, 624625, 627 Pronamide . . . . . . . . . . . . . . . . . . .356, 358, 367 Propachlor . . . . . . . . . . . . . . .350352, 355, 367 Propadiene . . . . . . . . . . . . . . .426427, 428429 Propanal . . . . . . . . . . . . . . . . . . . . . . . . . . . . .379 Propane . . . . . . . . . . . . .402, 425431, 433, 438 1,3-Propanediol . . . . . . . . . . . . . . . . . . .403404 1,2-Propanediol (propylene glycol) . . . . . .403-404 1-Propanethiol . . . . . . . . . . . . . . . . . . . .432, 434 2-Propanethiol . . . . . . . . . . . . . . . . . . . .432, 434 Propanil . . . . . . . . . . . . .358, 596, 624625, 627 Propanoic acid . . . . . . . . . . . . . . . . . . . . . . . .397 1-Propanol . . . . . . .372, 388, 394395, 407-408, 422424, 436 2-Propanol . . . . . . . . . . . . . . . . . . . . . . . . . . .436 Propanol . . . . . . . . . . . . . . . . . . . . . . . . . . . . .380 Propanolol . . . . . . . . . . . . . . . . . . . . . . .537, 606 2-Propanone . . . . . . . . . . . . . . . . . . . . . . . . . .436 Propargyl alcohol . . . . . . . . . . . . . . . . . . . . . .372 Propazine . . .356, 358359, 367, 596, 624625, 627628, 630 2-Propen-1-ol (allyl alcohol) . . . . . . . . . . . . .394 Propene . . . . . . . . . . . . . . . . . . . . . . . . . . . . .379 Propiolactone . . . . . . . . . . . . . . . . . . . . . . . . .372 Propiomazine . . . . . . . . . . . . . . . . . . . . . . . . .420 Propionaldehyde . . . . . . . . . . . . . . . . . .398, 630 Propionaldehyde-DNPH . . . . . . . . . . . . . . . .622 Propionic acid . . . . . . . . . . . . . . . .388, 390, 395 Propionitrile . . . . . . . . . . . . . .371372, 397, 409 Proposyphene . . . . . . . . . . . . . . . . . . . . . . . .416 Propranolol . . . . . . . . . . . . . . . . . . . . . . .605, 650 Propyl acetate . . . . . . . . . . . . . . . . . . . . . . . .402 n-Propylbenzene . . . . . .371, 373, 375, 401, 407, 430, 435436, 438439 Propylbenzene . . . .372, 399401, 406, 435, 437 Propyl benzoate . . . . . . . . . . . . . . . . . . . . . . .402 Propyl butyrate . . . . . . . . . . . . . . . . . . . . . . . .402 Propyl caproate . . . . . . . . . . . . . . . . . . . . . . .402 Propylene . . . . . . . . . . . .402, 426431, 433434 Propylene glycol . . . . . . . . . . . . . . . . . . . . . . .394 Propyl ether . . . . . . . . . . . . . . . . . . . . . . . . . .403 Propyl formate . . . . . . . . . . . . . . . . . . . . . . . .402 1-Propyl mercaptan . . . . . . . . . . . . . . . . . . . .434 2-Propyl mercaptan . . . . . . . . . . . . . . . . . . . .434 Propyl paraben . . . . . . . . . . . . . . . . . . . .634, 638 Propyl propionate . . . . . . . . . . . . . . . . . . . . . .402 Propyl valerate . . . . . . . . . . . . . . . . . . . . . . . .402 Propyne (methylacetylene) . . . . . .426429, 430 2-Propyn-1-ol (propargyl alcohol) . . . . . . . . .394 Proteins . . . . . . . . . . . . . . . . . . . . . .612, 615-617 Protocatechuic acid . . . . . . . . . . . . . . . . . . . .555 Prozac . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .576 Pseudoephedrine . . . . . . . . . . . . . .545, 606, 644 Pteropodin . . . . . . . . . . . . . . . . . . . . . . . . . . .649 Pulegone . . . . . . . . . . . . . . . . . . . . . . . . . . . . .385 Purines . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .652 Pyrazole . . . . . . . . . . . . . . . . . . . . . . . . . . . . .397 Pyrene . . . . . . . . . . . . . . . . . .366368, 626627 Pyrene-d10 . . . . . . . . . . . . . . . . . . . . . . . . . . .367 Pyrethrins . . . . . . . . . . . . . . . . . . . . . . . . . . . .364 Pyridate metabolite CL 9673 . . . . . . . . . . . . .630 Pyridazine . . . . . . . . . . . . . . . . . . . . . . . . . . . .397 Pyridine . . . . .366, 372, 397, 408409, 424, 546 Pyridoxine (B6) . . . . . . . . . . .531, 546, 641642 Pyrilamine . . . . . . . . . . . . . . .417, 530, 590, 644 Pyrimidine . . . . . . . . . . . . . . . . . . . . . . . .397, 652 Pyrithyldione . . . . . . . . . . . . . . . . . . . . . . . . .420 Pyrrobutamine . . . . . . . . . . . . . . . . . . . . . . . .415
Indices
Q
Quercetin . . . . . . . . . . . . . . . . . . . . . . . . . . . .527 Quinine . . . . . . . . . . . . . . . . . . . . . . . . . .421, 649
717
Compound Index
R
Raffinose . . . . . . . . . . . . . . . . . . . .592, 633, 637 RDX . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .624 Red #3 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .634 Red #40 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .634 Restriction Digest . . . . . . . . . . . . . . . . . . . . .593 Retinol . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .641 Retinol acetate . . . . . . . . . . . . . . . . . . . . . . . .641 Retinol, all-trans . . . . . . . . . . . . . . . . . . . . . . .641 Retinol palmitate . . . . . . . . . . . . . . . . . . . . . .641 Rhamnitol . . . . . . . . . . . . . . . . . . . . . . . . . . . .388 Rhamnose . . . . . . . . . . . . . . . . . . . . . . .592, 633 Rhynchophyllin . . . . . . . . . . . . . . . . . . . . . . . .649 Ribitol . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .388 Riboflavin (Vitamin B2) . . . . . . . . . . . . .641642 Ribonuclease . . . . . . . . . . . . . . . . . . . . . . . . .614 Ribose . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .633 D-Ribose anomers . . . . . . . . . . . . . . . . . . . . .389 Rimsulfuron-methyl . . . . . . . . . . . . . . . . . . . .630 RNA polymerase . . . . . . . . . . . . . . . . . . . . . .617 RNase . . . . . . . . . . .533, 535, 587, 611612, 616 Ronnel . . . . . . . . . . . . . . . . . . . . . . . . . .356357 Rosatol (rosetone) . . . . . . . . . . . . . . . . .381382 Rose oxide, cis-rose . . . . . . . . . . . . . . .381382 Rose oxide, trans-rose . . . . . . . . . . . . .381382 Strychnine . . . . . . . . . . . . . . . . . . . . . . . . . . .421 Styrene . . . . . . . . .371373, 378379, 399402, 406407, 410, 430, 435436 Styrene dimer . . . . . . . . . . . . . . . . . . . . . . . . .402 Styrene oxide . . . . . . . . . . . . . . . . . . . . . . . . .399 Styrene trimer . . . . . . . . . . . . . . . . . . . . . . . .402 Succinic Acid . . . . . . . . . . . . . . . . . . . . . . . . .621 Sucrose . . . . . . . . . . . . . . . . . . . . . .592, 633, 637 Sufentanyl . . . . . . . . . . . . . . . . . . . . . . . . . . .419 Sugar alcohols . . . . . . . . . . . . . . . . . . . . . . . .592 Sugars . . . . . . . . . . . . . . . . . . . . . . . . . . .389, 592 Sulfallate . . . . . . . . . . . . . . . . . . . . . . . . . . . .358 Sulfamethoxazole . . . . . . . . . . . . . . . . . . . . .538 Sulfotepp . . . . . . . . . . . . . . . . . . . . . . . .356357 Sulfur (in air) . . . . . . . . . . . . . . . . . . . . . . . . .380 Sulfur (in garlic) . . . . . . . . . . . . . . . . . . . . . . .389 Sulfur Compounds . . . . . . . . . . . . . . . . .432434 Sulfur dioxide (SO2) . . . . . . . . . . . . . . . .414, 433 Sulfur hexafluoride . . . . . . . . . . . . . . . . . . . .414 Sulpiride . . . . . . . . . . . . . . . . . . . . . . . . . . . . .420 Surfactant . . . . . . . . . . . . . . . . . . . . . . . . . . . .574 Sunflower Oil, Aged . . . . . . . . . . . . . . . . . . . .635 Sunset yellow . . . . . . . . . . . . . . . . . . . . . . . . .634 Syringic acid . . . . . . . . . . . . . . . . . . . . . . . . . .555 Tetrabutyltin . . . . . . . . . . . . . . . . . . . . . . . . . .365 Tetracaine . . . . . . . . . . . . . . . . . . . .417, 526, 643 1,2,4,5-Tetrachlorobenzene . . . . . . . . . .399400 2,2',4,4'-Tetrachlorobiphenyl . . . . . . . . . . . . .367 Tetrachlorodibenzo-p-furans . . . . . . . . . . . . .359 1,1,1,2-Tetrachloroethane . . .371373, 375, 405 1,1,2,2-Tetrachloroethane . . . . . . . . . . .371373, 378379, 405 Tetrachloroethene . . . . .371373, 375379, 405 Tetrachloroethylene . . . . . . . . . . . . . . . . . . . .377 2,3,4,5-Tetrachlorophenol . . . . . . . . . . . . . . .369 2,3,4,6-Tetrachlorophenol . . . . . . . . . . .369, 629 2,3,5,6-Tetrachlorophenol . . . . . . . . . . . . . . .369 2,4,5,6-Tetrachloro-m-xylene . . . . . . . . .353354 Tetrachloro-m-xylene . . . . . . .355, 363, 349-350 Tetrachlorvinphos (Stirifos) . . . . . . . . . . . . . .367 n-Tetracontane . . . . . . . . . . . . . . . . . . . . . . . .439 Tetracosane . . . . . . . . . . . . . . . . . . . . . .348, 439 Tetracycline . . . . . . . . . . . . . . . . . . . . . .649650 Tetradecane . . . . . . . . . . . . . . . . . .348, 438439 Tetradecanoic acid . . . . . . . . . . . . . . . . . . . . .390 n-Tetradecanylamine . . . . . . . . . . . . . . . . . . .396 Tetraethylenepentamine . . . . . . . . . . . .396-397 1,2,3,4-Tetrahydrofluoranthrene . . . . . . . . . .368 1,1,1,2-Tetrafluoroethane . . . . . . . . . . . . . . . .380 1,1,2,2-Tetrafluoroethane . . . . . . . . . . . . . . . .380 Tetrahydrofuran (THF) . . . . . . . . .371, 379, 403, 407408, 424 Tetrahydropyran . . . . . . . . . . . . . . . . . . . . . . .403 Tetrahydrothiophene . . . . . . . . . . . . . . . . . . .432 1,2,3,5-Tetramethylbenzene . . . . . . . . .438439 1,2,4,5-Tetramethylbenzene . . . . . . . . .437439 Tetrapentyltin . . . . . . . . . . . . . . . . . . . . . . . . .365 Tetryl . . . . . . . . . . . . . . . . . . . . . . . . . . . .623624 9-THC . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .422 L-11-Hydroxy-9-THC . . . . . . . . . . . . . . . . . .422 L-9-Carboxy-11-nor-9-THC . . . . . . . . . . . . .422 Thenyldiamine . . . . . . . . . . . . . . . . . . . . . . . .415 Theobromine . . . . . . . . . . . . . . . . . . . . . . . . .545 Theophylline . . . . . . . . . . . . . . . . . . . . . .545, 590 Thiamine (Vitamin B1) . . . . . . . . .531, 641642 Thiamylal . . . . . . . . . . . . . . . . . . . . . . . . . . . .418 Thifensulfuron-methyl . . . . . . . . . . . . . . . . . .630 4,4'-Thiodianiline . . . . . . . . . . . . . . . . . . . . . .411 Thionazin . . . . . . . . . . . . . . . . . . . . . . . .356357 Thiopental . . . . . . . . . . . . . . . . . . . . . . . . . . .418 Thiophene . . . . . . . . . . . . . . . . . . . . . . .432, 434 Thiophene and sec-Butyl mercaptan . . . . . .434 Thioridazine . . . . . . . . . . . . . . . . . . . . . . . . . .418 Thonzylamine . . . . . . . . . . . . . . . . . . . . . . . . .417 Threonine (Thr) . . . . . . . . . . . . . . . . . . . . . . .604 -Thujone . . . . . . . . . . . . . . . . . . . . . . . .381, 384 -Thujone . . . . . . . . . . . . . . . . . . . . . . . . . . . .384 Thujone . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .383 Thymine . . . . . . . . . . . . . . . . . . . . . . . . . . . . .652 Thymol . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .633 Thyroglobulin . . . . . . . . . . . . . . . . .587, 614, 616 Tillam (Pebulate) . . . . . . . . . . . . . . . . . . . . . .367 Tocainamide . . . . . . . . . . . . . . . . . . . . . . . . . .649 -Tocopherol . . . . . . . . . . . . .419, 636, 640641 -Tocopherol . . . . . . . . . . . . . . . . .419, 636, 640 -Tocopherol . . . . . . . . . . . . . .419, 636, 640, 641 -Tocopherol . . . . . . . . . . . . . .419, 636, 640-641 -Tocopherol (Vitamin E) . . . . . . . . . . . . . . .641 -Tocopherol Acetate . . . . . . . . . . . . . . . . . .641
T
2,4,5-T . . . . . . . . . . . . . . . . . . . . . . .357358, 629 2,4,5-T,P . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .357 TAA . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .436 Talbutal . . . . . . . . . . . . . . . . . . . . . . . . . .418, 420 TAME . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .436 Tartrazine . . . . . . . . . . . . . . . . . . . . . . . . . . . .634 Tatrazine . . . . . . . . . . . . . . . . . . . . . . . . . . . . .638 1,2,8,9-TCDD . . . . . . . . . . . . . . . . . . . . . . . . . .360 1,3,6,8-TCDD . . . . . . . . . . . . . . . . . . . . . . . . . .360 2,3,7,8-TCDD . . . . . . . . . . . . . . . . . . . . . . . . . .360 1,2,8,9-TCDF . . . . . . . . . . . . . . . . . . . . . . . . . .360 1,3,6,8-TCDF . . . . . . . . . . . . . . . . . . . . . . . . . .360 2,3,7,8-TCDF . . . . . . . . . . . . . . . . . . . . . . . . . .360 TCP (Tenocyclidine) . . . . . . . . . . . . . . . . . . . .420 Tebuthion . . . . . . . . . . . . . . . . . . . . . . . . . . . .627 Tebuthiuron . . . . . . . . . .356, 367, 624625, 627 Temazepam . . . . . . . . . . . . . . . . . . . . . . . . . .419 TEPP . . . . . . . . . . . . . . . . . . . . . . . . . . . .356357 Terbacil . . . . . . . . . . . . . . . . . . . . . .356, 358, 367 Terbufos . . . . . . . . . . . . . . . . . . . . .356357, 367 Teburthiron . . . . . . . . . . . . . . . . . . . . . . . . . . .596 Terbuthylazine . . . . . . . . . . . . . . . .358359, 628 Terbutryn . . . . . . . . . . . . . . . .356, 358, 628, 630 Terbutryne . . . . . . . . . . . . . . . . . . . . . . . . . . . .359 Terbutylazine . . . . . . . . . . . . . . . . . . . . .625, 628 Terphenyl-d14 . . . . . . . . . . . . . . . . . . . . . . . . .366 Terpinen-4-ol . . . . . . . . . . . . . . . . .381382, 387 -Terpinene . . . . . . . . . . . . . . . . . . . . . .384386 -Terpinene . . . . . . . . . . . . . . .381382, 386387 Terpinene . . . . . . . . . . . . . . . . . . . . . . . . . . . .385 Terpinene-4-ol . . . . . . . . . . . . . . . . . . . . . . . .387 -Terpineol . . . . . . . . . . . . . . .381383, 385387 -Terpinolene . . . . . . . . . . . . . . . . . . . . . . . . .385 Terpinolene . . . . . . . . . . . . . . . . . . .382, 384, 386 Terrazole . . . . . . . . . . . . . . . . . . . . . . . . . . . . .350 Terrazole (Etridazole) . . . . . . . . . . . . . . . . . . .367 Testosterone . . . . . . . . . . . . . . . . . . . . . . . . . .422 Tetraborane . . . . . . . . . . . . . . . . . . . . . . . . . .414
S
S-100 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .533 S-100 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .533 trans-Sabiene hydrate . . . . . . . . . . . . . .385386 Sabinene . . . . . . . . . . . . . . . . . . . .381, 384386 Saccharos . . . . . . . . . . . . . . . . . . . . . . . . . . . .635 Saint Johns Wort, Extract . . . . . . . . . . . . . .648 Salbutamol . . . . . . . . . . . . . . . . . . . . . . . . . . .646 Salicylamide . . . . . . . . . . . . . . . . . . . . . .417, 618 Salicylic acid . . . . . . . . . . . . . . . . . . . . . . . . .643 Sarcosine (SAR) . . . . . . . . . . . . . . . . . . . . . . .604 Scarlet Red . . . . . . . . . . . . . . . . . . . . . . .634, 638 Scopolamine . . . . . . . . . . . . . . . . . . . . .545, 644 Sebutylazine . . . . . . . . . . . . . . . . . . . . . .625, 628 Secbumeton . . . . . . . . . . . . . . . . . . . . . .358359 Secobarbital . . . . . . . . . . . . . . . . . . . . . .415, 418 Selenide . . . . . . . . . . . . . . . . . . . . . . . . . . . . .414 Selenium (in garlic) . . . . . . . . . . . . . . . . . . . .389 Semivolatiles, EPA . . . . . . . . . . . . . . . . .366370 Serine (Ser) . . . . . . . . . . . . . . . . . . . . . . . . . .604 Simazine . . . . . . . . . . . .351352, 356359, 367, 596, 624625, 628630 Simetryn . . . . . . . . . . . . . . . . . . . . .356, 358, 367 Simetryne . . . . . . . . . . . . . . . . . . . . . . . . . . . .359 -Sinensal . . . . . . . . . . . . . . . . . . . . . . . . . . .385 -Sinensal . . . . . . . . . . . . . . . . . . . . . . . . . . .385 Soamyl alcohol . . . . . . . . . . . . . . . . . . . . . . . .423 Solvents . . . . . . . . . . . . . . . . . . . . .406409, 424 Sorbic acid . . . . . . . . . . . . . . . . . . . . . . .632, 640 Sorbitol . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .636 Spectiophyllin . . . . . . . . . . . . . . . . . . . . . . . . .649 Stanozolol . . . . . . . . . . . . . . . . . . . . . . . . . . . .422 Stearic acid methyl ester (C18:0) . . . . . . . . .392 Steroids . . . . . . . . . . . . . . . . . . . . .421422, 649 Stigmasterol . . . . . . . . . . . . . . . . . . . . . . . . . .421 Stirophos . . . . . . . . . . . . . . . . . . . . . . . .356357 Strawberry aldehyde . . . . . . . . . . . . . . . . . . .388
Indices
718
Compound Index
Tokuthion . . . . . . . . . . . . . . . . . . . . . . . .356357 o-Tolidine . . . . . . . . . . . . . . . . . . . . . . . . . . . .411 Tolmetin . . . . . . . . . . . . . . . . . . . . . . . . . . . . .644 Tolperison . . . . . . . . . . . . . . . . . . . . . . . . . . . .647 m-Tolualdehyde . . . . . . . . . . . . . . . . . . . . . . .398 o-Tolualdehyde . . . . . . . . . . . . . . . . . . . . . . . .398 p-Tolualdehyde . . . . . . . . . . . . . . . . . . . . . . . .398 m-Tolualdehyde-DNPH . . . . . . . . . . . . . . . . .622 Toluene . . . . .347348, 371373, 375, 378379, 399402, 406408, 423424, 428430, 435, 437439 Toluene-d8 . . . . . . . . . . . . . . . . . . . . . . .372, 378 m-Toluic acid . . . . . . . . . . . . . . . . . . . . . . . . .644 m-Toluidin . . . . . . . . . . . . . . . . . . . . . . . . . . . .622 m-Toluidine . . . . . . . . . . . . . . . . . . . . . . . . . . .622 o-Toluidine . . . . . . . . . . . . . . . . . . . . . . . . . . .411 Tonalid . . . . . . . . . . . . . . . . . . . . . . . . . .381383 Toxaphene . . . . . . . . . . . . . . . . . . . . . . . . . . .364 2,4,5-TP . . . . . . . . . . . . . . . . . . . . . . . . . .358, 629 Trehalulose . . . . . . . . . . . . . . . . . . . . . . . . . . .635 Trepinen-4-ol . . . . . . . . . . . . . . . . . . . . .384386 -Trepinene . . . . . . . . . . . . . . . . . . . . . . . . . . .384 -Trepineol . . . . . . . . . . . . . . . . . . . . . . . . . . .384 Triademefon . . . . . . . . . . . . . . . . . . . . . . . . . .356 Triadimefon . . . . . . . . . . . . . . . . . . . . . . . . . . .367 Triamcinolone . . . . . . . . . . . . . . . . . . . . . . . . .652 Triazines . . . . . . . . . . . . . . . . . . . . . . . . . . . . .630 Triazolam . . . . . . . . . . . . . . . . . . . . . . . .416, 419 Tribenuron-methyl . . . . . . . . . . . . . . . . . . . . .630 Tribromoacetic acid . . . . . . . . . . . . . . . . . . . .370 2,4,6-Tribromophenol . . . . . . . . . . . . . . . . . . .366 Tributylpentyltin . . . . . . . . . . . . . . . . . . . . . . .365 Trichloroacetic acid . . . . . . . . . . . . . . . . . . . .370 Trichloroacetonitrile . . . . . . . . . . . . . . .376377 2,4,5-Trichloroaniline . . . . . . . . . . . . . . . . . . .411 1,2,3-Trichlorobenzene . . . .371373, 375, 399400 1,2,4-Trichlorobenzene . . . . .366, 371373, 375, 378379, 399400 1,3,5-Trichlorobenzene . . . . . . . . . . . . .399400 2,4,5-Trichlorobiphenyl . . . . . . . . . . . . . . . . . .367 1,1,1-Trichloroethane . . .371373, 375379, 405 1,1,2-Trichloroethane . . . . . . . . . .371373, 375, 377379, 405 1,1-Trichloroethane . . . . . . . . . . . . . . . . . . . .423 Trichloroethane . . . . . . . . . . . . . . . . . . . . . . .407 Trichloroethene . . . . . . .371373, 375379, 405 Trichloroethylene . . . . . . . . . . . . . .377, 408, 424 Trichlorofluoromethane (Freon 11) . . .371373, 375, 378, 379380 Trichloronate . . . . . . . . . . . . . . . . . . . . .356357 2,3,4-Trichlorophenol . . . . . . . . . . . . . . . . . . .369 2,3,5-Trichlorophenol . . . . . . . . . . . . . . . . . . .369 2,3,6-Trichlorophenol . . . . . . . . . . . . . . . . . . .369 2,4,5-Trichlorophenol . . . . . . . . . . . . . . .366, 369 2,4,6-Trichlorophenol . . . . . . .366, 369, 412, 629 3,4,5-Trichlorophenol . . . . . . . . . . . . . . . . . . .369 3,4,5-Trichlorophenoxy acetic acid . . . . . . . .629 3,4,5-Trichlorophenoxy proprionic acid . . . .629 1,2,3-Trichloropropane . . . .370371, 373, 377, 405 1,1,1-Trichloropropanone . . . . . . . . . . . . . . . .376 1,1,2-Trichlorotrifluoroethane (Freon 113) . . . . . . . . . . . . . . . . . . . . . . . .405 1,1,1-Trichloro-2,2,2-trifluroethane (Freon 113) . . . . . . . . . . . . . . . . . . . . . . . .413 1,1,2-Trichloro-1,2,2-trifluoroethane (Freon 113) . . . . . . . . . . . . . . . . . . . .378380 1,1,1-Trichloro-2-propanone . . . . . . . . . . . . .377 Tricosanoic acid methyl ester (C23:0) . . . . .392 Tricyclazole . . . . . . . . . . . . . . . . . . .356, 358, 367 Tricyclic Antidepressants . . . . . . . . . . . . . . .650 Tricyclic Antipsychotics . . . . . . . . . . . . . . . .418 Tridecane . . . . . . . . . . . . . . . . . . . . . . . .435, 437 Tridecanoic acid methyl ester (C13:0) . . . . .392 Triethylamine . . . . . . . . . . . . . . . . . . . . .397, 409 Triethylene glycol . . . . . . . . . . . . . . . . . . . . . .404 Triethylenetetramine . . . . . . . . . . . . . . .396397 1,1,1-Trifluoroethane . . . . . . . . . . . . . . . . . . .380 Trifluoromethane . . . . . . . . . . . . . . . . . . . . . .380 Trifluorotoluene . . . . . . . . . . . . . . . . . . .347, 373 Triflupromazine . . . . . . . . . . . . . . . . . . . . . . .418 Trifluralin . . . . . . . . . . . . . . . .350352, 355, 367 Triglycerides . . . . . . . . . . . . . . . . . . . . . . . . . .393 Triglyme (triethylene glycol dimethyl ether) . . . . . . .403 Trimethylamine . . . . . . . . . . . . . . . . . . . . . . .396 2,4,5-Trimethylaniline . . . . . . . . . . . . . . . . . . .411 1,2,3-Trimethylbenzene . . . . . . . . .347, 437439 1,2,4-Trimethylbenzene . . . .347348, 371373, 375, 378379, 406, 430, 437439 1,3,5-Trimethylbenzene . . . .347, 371373, 375, 378379, 430, 438439 1,2,4-Trimethylbenzene (pseudocumene) 399400 1,2,3-Trimethylbenzene (hemimellitene) . . .399400 1,3,5-Trimethylbenzene (mesitylene) . . . . . .400 3,5,5-Trimethylhexanol . . . . . . . . . . . . .381382 2,3,3-Trimethylpentane . . . . . . . . . . . . .437438 2,3,4-Trimethylpentane . . . . . . . . . . . . . . . . .430 2,2,4-Trimethylpentane (Isooctane) . . . . . . .379 2,3,5-Trimethylphenol . . . . . . . . . . . . . . . . . .413 Trimipramine . . . . . . . . . . . . .416, 605606, 650 1,3,5-Trinitrobenzene . . . . . . . . . . . . . . .623624 2,4,6-Trinitrophenol . . . . . . . . . . . . . . . . . . . .412 2,4,6 Trinitrotoluene . . . . . . . . . . . . . . . . . . . .623 2,4,6-Trinitrotoluene . . . . . . . . . . . . . . . . . . . .624 Tri-o-cresyl phosphate . . . . . . . . . . . . . .356357 Tripelennamine . . . . . . . . . . . . . . . . . . . . . . .417 Triphenylphosphate . . . . . . . . . . . . . . . . . . . .367 Triprolidine . . . . . . . . . . . . . . .417, 542, 545, 556 Triton X-114 . . . . . . . . . . . . . . . . . . . . . . . . . .620 Tyrosine (Tyr) . . . . . . . . . . . . . . . . . . . . . . . . .604 Vernolate . . . . . . . . . . . . . . . . . . . .356, 358, 367 Vertenex (isomer 1) . . . . . . . . . . . . . . . .381382 Vertenex (isomer 2) . . . . . . . . . . . . . . . .381382 Vinclozolin . . . . . . . . . . . . . . . . . . . . . . . . . . .640 Vinyl acetate . . . . . . . . . . . . . . . . . . . . .379, 402 Vinyl chloride . . . . . . . . .371373, 375, 378379 3-Vinyl-1,2-dithiin . . . . . . . . . . . . . . . . . . . . . .384 2-Vinyl-1,3-dithiin . . . . . . . . . . . . . . . . . . . . . .384 4-Vinylcyclohexene-1 dimer . . . . . . . . .428429 Viridiflorol . . . . . . . . . . . . . . . . . . . . . . . .385386 Vitamin A-palmitate . . . . . . . . . . . . . . . . . . . .641 Vitamin B1 (Thiamine) . . . . .531, 632, 641642 Vitamin B6, Pyridoxol Hydrochloride . . . . . .632 Vitamin B12 (Cyanocobalamin) . . . . . .614, 632 Vitamin C . . . . . . . . . . . . . . . . . . . . . . . .641642 Vitamin D . . . . . . . . . . . . . . . . . . . . . . . . . . . .641 Vitamin D3 . . . . . . . . . . . . . . . . . . . . . . . . . . .641 Vitamin K1 . . . . . . . . . . . . . . . . . . . . . . . . . . .641 Vitamins . . . . . . . . . . . . . . . . . . . . . . . . .640642 Volatiles . . . . . . . . . . . . . . . . . . . . . . . . . . . . .630 Volatiles, EPA . . . . . . . . . . . . . . . . . . . . .371377
W
Water . . . . . . . . . . . . . . . . . . . . . . .395, 425, 436
X
Xenon . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .425 Xylitol . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .592 Xylose . . . . . . . . . . . . . . . . . . . . . . .389, 592, 633 XMP . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .608 m-Xylene . . . . . .347, 348, 371373, 375, 378379, 399401, 406408, 423, 430, 435439 o-Xylene . . . . . . .347348, 371373, 375, 378379, 399401, 406408, 423, 430, 435439 p-Xylene . . . . . . .347, 348, 371373, 375, 378379, 399401, 406408, 423, 430, 435439 2,3-Xylenol . . . . . . . . . . . . . . . . . . . . . . .412413 2,4-Xylenol . . . . . . . . . . . . . . . . . . . . . . .412413 2,5-Xylenol . . . . . . . . . . . . . . . . . . . . . . .412413 2,6-Xylenol . . . . . . . . . . . . . . . . . . . . . . .412413 3,4-Xylenol . . . . . . . . . . . . . . . . . . . . . . .412413 3,5-Xylenol . . . . . . . . . . . . . . . . . . . . . . .412413 Xylitol . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .636
U
Uncarin F . . . . . . . . . . . . . . . . . . . . . . . . . . . .649 Undecane . . . . . . . . . . . . . . . .406, 435, 437-439 Undecanoic acid methyl ester (C11:0) . . . . .392 Uracil . . . . . . . . . . . . . . . . . . . . . . . .537, 618, 652 Uracil mono phosphate (UMP) . . . . . . . . . . .608 Urea . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .563
Y
Yellow #5 . . . . . . . . . . . . . . . . . . . . . . . . . . . .634 Yellow #6 . . . . . . . . . . . . . . . . . . . . . . . . . . . .634 Yogurt, Blueberry . . . . . . . . . . . . . . . . . . . . . .637 Yogurt, Blueberry, Fat-Free . . . . . . . . . . . . . .637 Yogurt, Plain . . . . . . . . . . . . . . . . . . . . . . . . . .637
V
Valencene . . . . . . . . . . . . . . . . . . . . . . . . . . . .385 Valeraldehyde (pentanal) . . . . . . . . . . .398, 630 Valeraldehyde-DNPH . . . . . . . . . . . . . . . . . . .622 iso-Valeric acid . . . . . . . . . . . . . . . . . . . . . . . .397 Valeric acid (pentanoic acid) . . . .390, 395, 397 Valerolactone . . . . . . . . . . . . . . . . . . . . . . . . .395 Valine (Val) . . . . . . . . . . . . . . . . . . . . . . . . . . .604 Valine-tyrosine-valine-tripeptide (val-tyr-val) . . .612 Vanillic acid . . . . . . . . . . . . . . . . . . . . . . . . . .555 Vanillin . . . . . . . . . . . . . . . . . . . . . . . . . .383, 634 Vanillin alcohol . . . . . . . . . . . . . . . . . . . . . . . .634
Indices
719
Part Number Index

009-1707 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .276 009-4003 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .129 009-4004 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .129 009-4005 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .129 009-5007 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .276 009-5017 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .276 0100-0091 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .84 0100-0118 . . . . . . . . . . . . . . . . . . . . . . . . . .81, 85 0100-0119 . . . . . . . . . . . . . . . . . . . . . . . . . .81, 85 0100-0121 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .85 0100-0124 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .84 0100-0126 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .84 0100-0128 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .84 0100-0132 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .84 0100-0133 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .84 0100-0160 . . . . . . . . . . . . . . . . . . . . . . . . . . . .205 0100-0161 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .84 0100-0241 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .85 0100-0420 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .83 0100-0542 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .84 0100-0782 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .84 0100-0787 . . . . . . . . . . . . . . . . . . . . . . . . . . . .205 0100-0900 . . . .84, 447, 467, 478, 492493, 497 0100-0969 . . . . . . . . . . . . . . . . . . . . . . . . .84, 492 0100-0985 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .84 0100-1017 . . . . . . . . . . . . . . . . . . . . . . . . . . . .493 0100-1048 . . . . . . . . . . . . . . . . . . . . . . . . . . . .492 0100-1104 . . . . . . . . . . . . . . . . . . . . . . . . . . . .163 0100-1224 . . . . . . . . . . . . . . . . . . . . . . . . . . . .693 0100-1225 . . . . . . . . . . . . . . . . . . . . . . . .693, 697 0100-1316 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .84 0100-1324 . . . . . . . . . . . . . . . . . . . . . . . . . . . .163 0100-1325 . . . . . . . . . . . . . . . . . . . . . . . . . . . .163 0100-1326 . . . . . . . . . . . . . . . . . . . . . . . . . . . .163 0100-1327 . . . . . . . . . . . . . . . . . . . . . . . . . . . .197 0100-1329 . . . . . . . . . . . . . . . . . . . . . . . . . . . .197 0100-1331 . . . . . . . . . . . . . . . . . . . . . . . .163, 194 0100-1332 . . . . . . . . . . . . . . . . . . . .163, 185, 194 0100-1342 . . . . . . . . . . . . . . . . . . . . . . . . . . . .163 0100-1344 . . . . . . . . . . . . . . . . . . . . . . . . . . . .163 0100-1365 . . . . . . . . . . . . . . . . . . . . . . . . . . . .197 0100-1375 . . . . . . . . . . . . . . . . . . . . . . . .163, 314 0100-1378 . . . . . . . . . . . . . . . . . . . . . . . . . . . .163 0100-1379 . . . . . . . . . . . . . . . . . . . . . . . . . . . .163 0100-1381 . . . . . . . . . . . . . . . . . . . . . . . . . . . .163 0100-1389 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .45 0100-1443 . . . . . . . . . . . . . . . . . . . . . . . . . . . .484 0100-1504 . . . . . . . . . . . . . . . . . . . . . . . . . . . .197 0100-1505 . . . . . . . . . . . . . . . . . . . . . . . . . . . .197 0100-1511 . . . . . . . . . . . . . . . . . . . . . . . . . . . .167 0100-1512 . . . . . . . . . . . . . . . . . . . . . . . . . . . .167 0100-1513 . . . . . . . . . . . . . . . . . . . . . . . . . . . .167 0100-1514 . . . . . . . . . . . . . . . . . . . . . . . . . . . .167 0100-1515 . . . . . . . . . . . . . . . . . . . . . . . . . . . .167 0100-1516 . . . . . . . . . . . . . . . . . . . .481, 490, 500 0100-1527 . . . . . . . . . . . . . . . . . . . . . . . . . . . .167 0100-1543 . . . . . . . . . . . . . . . . . . . . . . . . . . . .657 0100-1619 . . . . . . . . . . . . . . . . . . . . . . . . . . . .484 0100-1631 0100-1710 0100-1849 0100-1850 0100-1851 0100-1852 0100-1853 0100-1854 0100-1855 0100-1859 0100-1860 0100-1921 0100-1922 0100-1923 0100-1924 0100-2051 0100-2087 0100-2088 0100-2089 0100-2175 0101-0030 0101-0113 0101-0282 0101-0299 0101-0300 0101-0301 0101-0302 0101-0303 0101-0355 0101-0376 0101-0377 0101-0378 0101-0379 0101-0403 0101-0584 0101-0585 0101-0594 0101-0595 0101-0607 0101-0620 0101-0623 0101-0624 0101-0626 0101-0627 0101-0633 0101-0636 0101-0637 0101-0638 0101-0639 0101-0655 0101-0656 0101-0657 0101-0658 0101-0663 0101-0670 0101-0727 0101-0920 0101-0921 . . . . . . . . . . . . . . . . . . . . . . . . . . . .447 . . . . . . . . . . . . . . . . . . . .458, 460, 695 . . . . . . . . . . . . . . . . . . . . . . . . . . . .466 . . . . . . . . . . . . . . . . . . . . . . . . . . . .466 . . . . . . . . . . . . . . . . . . . . . . . .466, 471 . . . . . . . . . . . . . . . . . . . . . . . .466, 471 . . . . . . . . . . . . . . . . . . . . . . . . . . . .466 . . . . . . . . . . . . . . . . . . . . . . . . . . . .471 . . . . . . . . . . . . . . . . . . . . . . . . . . . .471 . . . . . . . . . . . . . . . . . . . . . . . . . . . .451 . . . . . . . . . . . . . . . . . . . . . . . . . . . .451 . . . . . . . . . . . . . . . . . . . . . . . . . . . .452 . . . . . . . . . . . . . . . . . . . . . . . . . . . .452 . . . . . . . . . . . . . . . . . . . . . . . . . . . .452 . . . . . . . . . . . . . . . . . . . . . . . . . . . .452 . . . . . . . . . . . . . . . . . . . . . . . . . . . .483 . . . . . . . . . . . . . . . . . . . . . . . . . . . .471 . . . . . . . . . . . . . . . . . . . . . . . . . . . .469 . . . . . . . . . . . . . . . . . . . . . . . .469, 471 . . . . . . . . . . . . . . . . . . . . . . . . . . . .447 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .79 . . . . . . . . . . . . . . . . . . . . . . . . . .72, 79 . . . . . . . . . . . . . . . . . . . . . . . .168, 470 . . . . . . . . . . . . . . . . . . . . . . . . . . . .168 . . . . . . . . . . . . . . . . . . . . . . . . . . . .168 . . . . . . . . . . . . . . . . . . . . . . . . . . . .168 . . . . . . . . . . . . . . . . . . . . . . . . . . . .168 . . . . . . . . . . . . . . . . . . . . . . . . . . . .168 . . . . . . . . . . . . . . . . . . . . . . . . . . . .168 . . . . . . . . . . . . . . . . . . . .452, 487, 496 . . . . . . . . . . . . . . . .452, 470, 487, 496 . . . . . . . . . . . . . . . .452, 470, 487, 496 . . . . . . . . . . . . . . . .452, 470, 487, 496 . . . . . . . . . . . . . . . . . . . . . . . . . . . .168 . . . . . . . . . . . . . . . . . . . . . . . . . . . .167 . . . . . . . . . . . . . . . . . . . . . . . . . . . .167 . . . . . . . . . . . . . . . . . . . . . . . . . . . .166 . . . . . . . . . . . . . . . . . . . . . . . . . . . .166 . . . . . . . . . . . . . . . . . . . . . . . .486, 496 . . . . . . . . . . . . . . . .451, 486, 488, 496 . . . . . . . . . . . . . . . . . . . .451, 486, 496 . . . . . . . . . . . . . . . . . . . . . . . .451, 486 . . . . . . . . . . . . . . . . . . . . . . . . . . . .487 . . . . . . . . . . . . . . . . . . . . . . . .487488 . . . . . . . . . . . . . . . . . . . . . . . . . . . .168 . . . . . . . . . . . . . . . . . . . . . . . . . . . .167 . . . . . . . . . . . . . . . . . . . . . . . . . . . .167 . . . . . . . . . . . . . . . . . . . . . . . . . . . .167 . . . . . . . . . . . . . . . . . . . . . . . . . . . .167 . . . . . . . . . . . . . . . . . . . . . . . . . . . .453 . . . . . . . . . . . . . . . . . . . . . . . . . . . .453 . . . . . . . . . . . . . . . . . . . . . . . . . . . .453 . . . . . . . . . . . . . . . . . . . . . . . . . . . .453 . . . . . . . . . . . . . . . . . . . . . . . . . . . .488 . . . . . . . . . . . . . . . . . . . . . . . . . . . .453 . . . . . . . . . . . . . . . . . . . . . . . . . . . .487 . . . . . . . . . . . . . . . . . . . . . . . . . . . .471 . . . . . . . . . . . . . . . . . . . . . . . . . . . .466 0101-1050 . . . . . . . . . . . . . . . . . . . . . . . . . . . .469 0101-1219 . . . . . . . . . . . . . . . . . . . .452, 487, 496 0101-1226 . . . . . . . . . . . . . . . . . . . . . . . . . . . .453 0101-1227 . . . . . . . . . . . . . . . . . . . . . . . . . . . .453 0101-1228 . . . . . . . . . . . . . . . . . . . . . . . . . . . .453 0101-1229 . . . . . . . . . . . . . . . . . . . . . . . . . . . .453 0101-1230 . . . . . . . . . . . . . . . . . . . . . . . . . . . .453 0101-1231 . . . . . . . . . . . . . . . . . . . . . . . . . . . .450 0101-1232 . . . . . . . . . . . . . . . . . . . . . . . . . . . .450 0101-1233 . . . . . . . . . . . . . . . . . . . . . . . . . . . .451 0101-1234 . . . . . . . . . . . . . . . . . . . . . . . . . . . .453 0101-1235 . . . . . . . . . . . . . . . . . . . . . . . . . . . .453 0101-1236 . . . . . . . . . . . . . . . . . . . . . . . . . . . .453 0101-1237 . . . . . . . . . . . . . . . . . . . . . . . . . . . .453 0101-1238 . . . . . . . . . . . . . . . . . . . . . . . . . . . .453 0101-1239 . . . . . . . . . . . . . . . . . . . . . . . . . . . .453 0101-1240 . . . . . . . . . . . . . . . . . . . . . . . . . . . .453 0101-1241 . . . . . . . . . . . . . . . . . . . . . . . . . . . .453 0101-1242 . . . . . . . . . . . . . . . . . . . . . . . . . . . .453 0101-1243 . . . . . . . . . . . . . . . . . . . . . . . . . . . .452 0101-1244 . . . . . . . . . . . . . . . . . . . . . . . . . . . .452 0101-1245 . . . . . . . . . . . . . . . . . . . . . . . . . . . .452 0101-1246 . . . . . . . . . . . . . . . . . . . . . . . . . . . .452 0101-1247 . . . . . . . . . . . . . . . . . . . . . . . . . . . .452 0101-1248 . . . . . . . . . . . . . . . . . . . . . . . . . . . .452 0101-1249 . . . . . . . . . . . . . . . . . . . . . . . . . . . .452 0101-1250 . . . . . . . . . . . . . . . . . . . .452, 487, 496 0101-1251 . . . . . . . . . . . . . . . . . . . . . . . .452, 487 0101-1252 . . . . . . . . . . . . . . . . . . . . . . . .452, 496 0101-1253 . . . . . . . . . . . . . . . . . . . . . . . . . . . .450 0101-1255 . . . . . . . . . . . . . . . . . . . . . . . . . . . .451 01010-80002 . . . . . . . . . . . . . . . . . . . . . . . . . .494 01018-22707 . . . . . . . . . . . . . . . . . . . . . .462, 485 01018-23702 . . . . . . . . . . . . . . . . . .458, 462, 485 01018-60007 . . . . . . . . . . . . . . . . . . . . . . . . . .485 01018-60010 . . . . . . . . . . . . . . . . . . . . . . . . . .485 01018-60017 . . . . . . . . . . . . . . . . . . . . . .485486 01018-60025 . . . . . . . . . . . . . . . . . .464, 485, 492 01018-67303 . . . . . . . . . . . . . . . . . . . . . . . . . .485 01018-67305 . . . . . . . . . . . . . . . . . .445, 459, 486 01018-68722 . . . . . . . . . . . . . . . . . . . . . .462, 485 01018-68723 . . . . . . . . . . . . . . . . . . . . . . . . . .485 01018-68724 . . . . . . . . . . . . . . . . . . . . . . . . . .485 01018-82701 . . . . . . . . . . . . . . . . . . . . . . . . . .485 01019-67305 . . . . . . . . . . . . . . . . . . . . . .459, 486 01019-82702 . . . . . . . . . . . . . . . . . . . . . .485, 493 01019-87608 . . . . . . . . . . . . . . . . . . . . . . . . . .486 01037-60002 . . . . . . . . . . . . . . . . . . . . . . . . . .499 01040-87602 . . . . . . . . . . . . . . . . . . . . . .445, 499 01046-28101 . . . . . . . . . . . . . . . . . . . . . . . . . .499 01046-28102 . . . . . . . . . . . . . . . . . . . . . . . . . .499 01046-28103 . . . . . . . . . . . . . . . . . . . . . . . . . .499 01046-60010 . . . . . . . . . . . . . . . . . . . . . . . . . .499 01046-60021 . . . . . . . . . . . . . . . . . . . . . . . . . .500 01046-60022 . . . . . . . . . . . . . . . . . . . . . . . . . .500 01046-60023 . . . . . . . . . . . . . . . . . . . . . . . . . .500 01046-60025 . . . . . . . . . . . . . . . . . . . . . . . . . .500 01046-60210 . . . . . . . . . . . . . . . . . . . . . . . . . .105
Indices
720
Part Number Index

01046-67101 01046-67102 01046-67103 01046-68702 01048-87302 01049-24705 01049-25701 01049-27305 01049-27306 01049-27604 01049-27708 01049-28801 01049-28802 01049-45201 01049-47102 01049-60013 01049-62901 01049-62902 01049-64105 01049-67001 01049-68700 01049-92101 01050-60000 01078-44501 01078-44511 01078-67200 01078-68704 01078-68705 01078-68721 01078-87303 01078-87305 01078-87306 01078-87307 01078-87308 01079-67101 01079-67201 01079-68721 01079-87303 01079-87306 01079-87307 01079-87308 01080-68702 01080-68704 01080-83202 01090-07101 01090-27606 01090-27609 01090-27611 01090-27613 01090-27614 01090-60006 01090-60008 01090-60009 01090-61105 01090-68701 01090-68702 01090-68703 01090-68704 . . . . . . . . . . . . . . . . . . . . . . . . . .499 . . . . . . . . . . . . . . . . . . . . . . . . . .499 . . . . . . . . . . . . . . . . . . . . . . . . . .499 . . . . . . . . . . . . . . . . . . . . . . . . . .499 . . . . . . . . . . . . . .445, 459, 486, 500 . . . . . . . . . . . . . . . . . . . . . . . . . .500 . . . . . . . . . . . . . . . . . . . . . . . . . .500 . . . . . . . . . . . . . . . . . . . . . . . . . .500 . . . . . . . . . . . . . . . . . . . . . . . . . .500 . . . . . . . . . . . . . . . . . . . . . . . . . .500 . . . . . . . . . . . . . . . . . . . . . . . . . .500 . . . . . . . . . . . . . . . . . . . . . . . . . .500 . . . . . . . . . . . . . . . . . . . . . . . . . .500 . . . . . . . . . . . . . . . . . . . . . . . . . .500 . . . . . . . . . . . . . . . . . . . . . . . . . .500 . . . . . . . . . . . . . . . . . . . . . . . . . .500 . . . . . . . . . . . . . . . . . . . . . . . . . .500 . . . . . . . . . . . . . . . . . . . . . . . . . .500 . . . . . . . . . . . . . . . . . . . . . . . . . .500 . . . . . . . . . . . . . . . . . . . . . . . . . .500 . . . . . . . . . . . . . . . . . . . . . . . . . .500 . . . . . . . . . . . . . . . . . . . . . . . . . .500 . . . . . . . . . . . . . . . . . . . . . . . . . .485 . . . . . . . . . . . . . . . . . . . . . . . . . .487 . . . . . . . . . . . . . . . . . . . . . . . . . .487 . . . . . . . . . . . . . . . . . . . . . . . . . .487 . . . . . . . . . . . . . . . . . . . . . . . . . .487 . . . . . . . . . . . . . . . . . . . . . . . . . .487 . . . . . . . . . . . . . . . . . . . . . . . . . .487 . . . . . . . . . . . . . . . . . . . . . .486487 . . . . . . . . . . . . . . . . . . . . . . . . . .486 . . . . . . . . . . . . . . . . . . . . . . . . . .486 . . . . . . . . . . . . . . . . . . . . . . . . . .487 . . . . . . . . . . . . . . . . . . . . . . . . . .487 . . . . . . . . . . . . . . . . . . . . . . . . . .488 . . . . . . . . . . . . . . . . . . . . . . . . . .488 . . . . . . . . . . . . . . . . . . . . . . . . . .488 . . . . . . . . . . . . . . . . . . . . . . . . . .488 . . . . . . . . . . . . . . . . . . . . . . . . . .486 . . . . . . . . . . . . . . . . . . . . . . . . . .488 . . . . . . . . . . . . . . . . . . . . . . . . . .488 . . . . . . . . . . . . . . . . . . . . . . .94, 482 . . . . . . . . . . . . . . . . . . . . . . .94, 482 . . . . . . . . . . . . . . . . . . . . . . .83, 492 . . . . . . . . . . . . . . . . . . . . . .497498 . . . . . . . . . . . . . . . . . . . . . . . . . .514 . . . . . . . . . . . . . . . . . . . . . . . . . .448 . . . . . . . . . . . . . . . . . . . . . . . . . .514 . . . . . . . . . . . . . . . . . . . . . . . . . .514 . . . . . . . . . . . . . . . . . . . . . . . . . .514 . . . . . . . . . . . . . . . . . . . . . . . . . .492 . . . . . . . . . . . . . . . . . . . . . .492493 . . . . . . . . . . . . . . . . . . . . . . . . . .492 . . . . . . . . . . . . . . . . . . . . . . . . . .493 . . . . . . . . . . . . . . . . . . . . . . . . . .498 . . . . . . . . . . . . . . . . . . . . . . . . . .448 . . . . . . . . . . . . . . . . . . . . . . . . . .448 . . . . . . . . . . . . . . . . . . . . . . . . . .494 01090-68721 . . . . . . . . . . . . . . . . . . . . . . . . . .492 01090-82701 . . . . . . . . . . . . . . . . . . . . . . . . . .492 01090-87301 . . . . . . . . . . . . . . . . . . . . . . . . . .493 01090-87303 . . . . . . . . . . . . . . . . . . . . . .493, 495 01090-87304 . . . . . . . . . . . . . . . . . .445, 459, 497 01090-87305 . . . . . . . . . . . . . . . . . . . . . .445, 459 01090-87307 . . . . . . . . . . . . . . . . . . . . . . . . . .498 01090-87308 . . . . . . . . . . . . . . . . . .445, 459, 472 01090-87317 . . . . . . . . . . . . . . . . . . . . . . . . . .493 01090-87322 . . . . . . . . . . . . . . . . . . . . . . . . . .498 01090-87603 . . . . . . . . . . . . . . . . . . . . . . . . . .494 01090-87606 . . . . . . . . . . . . . . . . . . . . . . . . . .493 01090-87610 . . . . . . . . . . . . . . . . . .445, 459, 496 01090-87611 . . . . . . . . . . . . . . . . . . . . . .445, 459 01090-87615 . . . . . . . . . . . . . . . . . . . . . . . . . .493 03394-20500 . . . . . . . . . . . . . . . . . . . . . . . . . .102 03394-60540 . . . . . . . . . . . . . . . . . . . . . . . . . .105 03394-60560 . . . . . . . . . . . . . . . . . . . . . .104105 03394-60600 . . . . . . . . . . . . . . . . . . . . . . . . . .105 03395-60540 . . . . . . . . . . . . . . . . . . . . . . . . . .105 03396-40050 . . . . . . . . . . . . . . . . . . . . . . . . . .102 03396-60500 . . . . . . . . . . . . . . . . . . . . . . . . . .105 03396-60550 . . . . . . . . . . . . . . . . . . . . . . . . . .105 03396-60560 . . . . . . . . . . . . . . . . . . . . . .104105 03396-60650 . . . . . . . . . . . . . . . . . . . . . .104105 03396-61010 . . . . . . . . . . . . . . . . . . . . . .104105 03396-61020 . . . . . . . . . . . . . . . . . . . . . . . . . .105 0350-1402 . . . . . . . . . . . . . . . . . . . . . . . . . . . .447 0460-0186 . . . . . . . . . . . . . . . . . . . . . . . . . . . .174 0460-1266 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .72 0515-0502 . . . . . . . . . . . . . . . . . . . . . . . . . . . .494 0515-0655 . . . . . . . . . . . . . . . . . . . . . . . . . . . .184 0515-0911 . . . . . . . . . . . . . . . . . . . . . . . . . . . .187 0515-1046 . . . . . . . . . . . . . . . . . . . . . . . .206207 0515-1056 . . . . . . . . . . . . . . . . . . . . . . . .479480 0515-1446 . . . . . . . . . . . . . . . . . . . . . . . .206207 0515-2495 . . . . . . . . . . . . . . . . . . . . . . . . . . . .184 0535-0071 . . . . . . . . . . . . . . . . . . . . . . . . . . . .206 05890-61090 . . . . . . . . . . . . . . . . . . . . . . . . . .168 05890-61095 . . . . . . . . . . . . . . . . . . . . . . . . . .168 05890-61260 . . . . . . . . . . . . . . . . . . . . . . . . . . .70 05921-21170 . . . . . . . . . . . . . . . . . . . . . .163, 201 05921-21850 . . . . . . . . . . . . . . . . . . . . . . . . . .196 05921-21860 . . . . . . . . . . . . . . . . . . . . . . . . . .196 05921-61090 . . . . . . . . . . . . . . . . . . . . . . . . . .196 05921-61390 . . . . . . . . . . . . . . . . . . . . . . . . . .196 05921-80350 . . . . . . . . . . . . . . . . . . . . . . . . . .196 05921-80600 . . . . . . . . . . . . . . . . . . . . . . . . . .196 05970-60045 . . . . . . . . . . . . . . . . . . . . . . . . . .216 05971-20126 . . . . . . . . . . . . . . . . . . . . . .206207 05971-20128 . . . . . . . . . . . . . . . . . . . . . . . . . .207 05971-20130 . . . . . . . . . . . . . . . . . . . . . .206207 05971-20134 . . . . . . . . . . . . . . . . . . . . . .206207 05971-20143 . . . . . . . . . . . . . . . . . . . . . .206207 05971-20305 . . . . . . . . . . . . . . . . . . . . . . . . . .207 05971-60053 . . . . . . . . . . . . . . . . . . . . . . . . . .207 05971-60102 . . . . . . . . . . . . . . . . . . . . . . . . . .207 05971-60140 . . . . . . . . . . . . . . . . . . . . . . . . . .207 05971-60170 . . . . . . . . . . . . . . . . . . . . . . . . . .207 05971-60226 . . . . . . . . . . . . . . . . . . . . . . . . . .207 05971-60561 . . . . . . . . . . . . . . . . . . . . . . . . . .206 05971-60571 . . . . . . . . . . . . . . .98, 205, 214, 216 05971-80102 . . . . . . . . . . . . . . . . . . . . . . . . . .210 05971-80103 . . . . . . . . . . . . . . . . . .210, 215, 483 05980-20018 . . . . . . . . . . . . . . . . . . . . . . . . . .205 05980-60051 . . . . . . . . . . . . . . . . . .205, 214, 483 05985-60179 . . . . . . . . . . . . . . . . . . . . . . . . . .214 05988-20066 . . . . . . . . . . . . . . . . . . . . . .163, 201 05988-20242 . . . . . . . . . . . . . . . . . . . . . . . . . .484 05989-20109 . . . . . . . . . . . . . . . . . . . . . . . . . .214 05989-20110 . . . . . . . . . . . . . . . . . . . . . . . . . .214 05989-20111 . . . . . . . . . . . . . . . . . . . . . . . . . .214 05989-20119 . . . . . . . . . . . . . . . . . . . . . . . . . .214 05989-20145 . . . . . . . . . . . . . . . . . . . . . . . . . .214 05989-20165 . . . . . . . . . . . . . . . . . . . . . . . . . .214 05989-20705 . . . . . . . . . . . . . . . . . . . . . . . . . .215 05989-60098 . . . . . . . . . . . . . . . . . . . . . . . . . .214 05989-60172 . . . . . . . . . . . . . . . . . . . . . . . . . .484 05989-67002 . . . . . . . . . . . . . . . . . . . . . . . . . .214 05989-80043 . . . . . . . . . . . . . . . . . . . . . . . . . .215 05990-60075 . . . . . . . . . . . . . . . . . . . . . . . . . .216 07673-20570 . . . . . . . . . . . . . . . . . . . . . . . . . .173 07673-40180 . . . . . . . . . . . . . . . . . . . . . . .32, 173 07673-60840 . . . . . . . . . . . . . . . . . . . . . . . . . .173 07675-80050 . . . . . . . . . . . . . . . . . . . . . . . . . .168 08450-60117 . . . . . . . . . . . . . . . . . . . . . . .93, 699 08450-60300 . . . . . . . . . . . . . . . . . . . . . . . . . . .94 08451-60104 . . . . . . . . . . . . . . . . . . . . . . . . . .696 08451-60302 . . . . . . . . . . . . . . . . . . . . . . . . . .696 08452-60104 . . . . . . . . . . . . . . . . . . . . . . . . . .697 0854-0141 . . . . . . . . . . . . . . . . . . . . . . . .187, 195 0890-1713 . . . . . . . . . . . . . . . . . . . . . . . . . . . .459 0890-1761 . . . . . . . . . . . . . . . . . . . . . . . .446, 500 0890-1762 . . . . . . . . . . . . . . . . . . . . . . . . . . . .446 0890-1763 . . . . . . . . . . . . . . . . . . . . . . . . . . . .446 0890-1901 . . . . . . . . . . . . . . . . . . . . . . . . . . . .484 0890-1915 . . . . . . . . . . . . . . . . . . . . . . . . . . . .446 0890-1921 . . . . . . . . . . . . . . . . . . . . . . . . . . . .484 0905-0970 . . . . . . . . . . . . . . . . . . . . . . . .493, 495 0905-1101 . . . . . . . . . . . . . . . . . . . . . . . . . . . .195 0905-1102 . . . . . . . . . . . . . . . . . . . . . . . .187, 195 0905-1103 . . . . . . . . . . . . . . . . . . . . . . . . . . . .195 0905-1145 . . . . . . . . . . . . . . . . . . . . . . . . . . . .215 0905-1159 . . . . . . . . . . . . . . . . . . . . . . . . . . . .197 0905-1160 . . . . . . . . . . . . . . . . . . . . . . . . . . . .197 0905-1161 . . . . . . . . . . . . . . . . . . . . . . . . . . . .196 0905-1162 . . . . . . . . . . . . . . . . . . . . . . . . . . . .197 0905-1163 . . . . . . . . . . . . . . . . . . . . . . . . . . . .197 0905-1175 . . . . . . . . . . . . . . . . . . . . . . . .462, 485 0905-1189 . . . . . . . . . . . . . . . . . . . . . . . . . . . .215 0905-1194 . . . . . . . . . . . . . . . . . . . . . . . .455, 494 0905-1259 . . . . . . . . . . . . . . . . . . . . . . . . . . . .492 0905-1284 . . . . . . . . . . . . . . . . . . . . . . . . . . . .184 0905-1294 . . . . . . . . . . . . . . . . . . . . . . . . . . . .467 0905-1420 . . . . . . . . . . . . . . . . . . . .455, 463, 485 0905-1516 . . . . . . . . . . . . . . . . . . . . . . . . . . . .465
Indices
721
Part Number Index

0905-2580 . . . . . . . . . . . . . . . . . . . . . . . . . . . .184 0960-0376 . . . . . . . . . . . . . . . . . . . . . . . . . . . .209 0960-0799 . . . . . . . . . . . . . . . . . . . . . . . . . . . .215 0960-0897 . . . . . . . . . . . . . . . . . . . . . . . . . . . .209 1000-0488 . . . . . . . . . . . . . . . .478479, 491, 498 1000-0544 . . . . . . . . . . . . . . . . . . . . . . . . . . . .692 1000-0953 . . . . . . . . . . . . . . . . . . . . . . . .479, 491 10833B-2310 . . . . . . . . . . . . . . . . . . . . . . . . .106 10833G-2310 . . . . . . . . . . . . . . . . . . . . . . . . .106 10834A-2310 . . . . . . . . . . . . . . . . . . . . . . . . .106 112-2032 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .295 112-2062 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .295 112-2112 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .296 112-2132 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .296 112-2133 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .296 112-2133E . . . . . . . . . . . . . . . . . . . . . . . . . . . .300 112-2162 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .296 112-2532 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .268 112-2562 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .268 112-3032 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .298 112-5132 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .295 112-5432 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .298 112-5462 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .298 112-6632 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .267 113-2032 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .295 113-2062 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .295 113-2112 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .296 113-2132 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .296 113-2132E . . . . . . . . . . . . . . . . . . . . . . . . . . . .300 113-2133 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .296 113-2532 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .268 113-3032 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .298 113-3062 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .298 113-3112 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .290 113-3132 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .290 113-3133 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .290 113-3162 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .290 113-3412 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .292 113-3422 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .292 113-3432 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .292 113-4302 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .289 113-4312 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .289 113-4332 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .289 113-4362 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .289 113-5132 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .295 113-5412 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .298 113-5432 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .298 113-6632 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .267 115-2112 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .296 115-2132 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .296 115-2162 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .296 115-2537 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .268 115-3113 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .290 115-3133 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .290 115-3332 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .287 115-3352 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .287 115-3412 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .292 115-3422 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .292 115-3432 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .292 115-3432E . . . . . . . . . . . . . . . . . . . . . . . . . . . .302 115-34H2 . . . . . . . . . . . . . . . . . . . . . . . . . . . .292 115-3532 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .289 115-3552 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .289 12-0507-024TMR . . . . . . . . . . . . . . . . . . . . . .170 121-0223 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .255 121-0713 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .259 121-0723 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .259 121-1012 . . . . . . . . . . . . . . . . . . . . . . . . .232, 307 121-1013 . . . . . . . . . . . . . . . . . . . . . . . . .232, 307 121-101A . . . . . . . . . . . . . . . . . . . . . . . . . . . .232 121-1022 . . . . . . . . . . . . . . . . . . . . . . . . .232, 307 121-1023 . . . . . . . . . . . . . . . . . . . . . . . . .232, 307 121-102A . . . . . . . . . . . . . . . . . . . . . . . . . . . .232 121-1043 . . . . . . . . . . . . . . . . . . . . . . . . .232, 307 121-1222 . . . . . . . . . . . . . . . . . . . . .227, 247, 310 121-1232 . . . . . . . . . . . . . . . . . . . . .227, 247, 310 121-1262 . . . . . . . . . . . . . . . . . . . . . . . . .227, 247 121-1313 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .258 121-1324 . . . . . . . . . . . . . . . . . . . . . . . . .257, 283 121-1524 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .282 121-1544 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .282 121-1712 . . . . . . . . . . . . . . . . . . . . . . . . .251, 309 121-1713 . . . . . . . . . . . . . . . . . . . . . . . . .251, 309 121-1722 . . . . . . . . . . . . . . . . . . . . . . . . .251, 309 121-1723 . . . . . . . . . . . . . . . . . . . . . . . . .251, 309 121-2023 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .255 121-2223 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .256 121-2323 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .254 121-4722 . . . . . . . . . . . . . . . . . . . . .229, 253, 310 121-5012 . . . . . . . . . . . . . . . . . . . . . . . . .239, 308 121-5013 . . . . . . . . . . . . . . . . . . . . . . . . .239, 308 121-5022 . . . . . . . . . . . . . . . . . . . . . . . . .239, 308 121-5023 . . . . . . . . . . . . . . . . . . . . . . . . .239, 308 121-5042 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .239 121-5043 . . . . . . . . . . . . . . . . . . . . . . . . .239, 308 121-5522 . . . . . . . . . . . . . . . . . . . . . . . . .223, 308 121-5542 . . . . . . . . . . . . . . . . . . . . .223, 243, 308 121-7012 . . . . . . . . . . . . . . . . . . . . . . . . .261, 309 121-7013 . . . . . . . . . . . . . . . . . . . . . . . . .261, 309 121-7022 . . . . . . . . . . . . . . . . . . . . . . . . .261, 309 121-7023 . . . . . . . . . . . . . . . . . . . . . . . . .261, 309 121-7043 . . . . . . . . . . . . . . . . . . . . . . . . .261, 309 122-0112 . . . . . . . . . . . . . . . . . . . . .222, 237, 308 122-0131 . . . . . . . . . . . . . . . . . . . . .222, 237, 308 122-0132 . . . . . . . . . . . . . . . . . . . . .222, 237, 308 122-0162 . . . . . . . . . . . . . . . . . . . . .222, 237, 308 122-0212 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .255 122-0232 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .255 122-0233 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .255 122-0711 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .259 122-0712 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .259 122-0713 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .259 122-0731 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .259 122-0732 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .259 122-0732E . . . . . . . . . . . . . . . . . . . . . . . . . . . .301 122-0733 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .259 122-0761 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .259 122-0762 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .259 122-0763 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .259 122-0766 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .259 122-1011 . . . . . . . . . . . . . . . . . . . . . . . . .233, 307 122-1012 . . . . . . . . . . . . . . . . . . . . . . . . .233, 307 122-1013 . . . . . . . . . . . . . . . . . . . . . . . . .233, 307 122-101E . . . . . . . . . . . . . . . . . . . . . . . . . . . . .233 122-1022 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .233 122-1031 . . . . . . . . . . . . . . . . . . . . . . . . .233, 307 122-1032 . . . . . . . . . . . . . . . . . . . . . . . . .233, 307 122-1032E . . . . . . . . . . . . . . . . . . . . . . . . . . . .300 122-1033 . . . . . . . . . . . . . . . . . . . . . . . . .233, 307 122-1033E . . . . . . . . . . . . . . . . . . . . . . . . . . . .300 122-103E . . . . . . . . . . . . . . . . . . . . . . . . . . . . .233 122-1052 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .233 122-1061 . . . . . . . . . . . . . . . . . . . . . . . . .233, 307 122-1062 . . . . . . . . . . . . . . . . . . . . . . . . .233, 307 122-1063 . . . . . . . . . . . . . . . . . . . . . . . . .233, 307 122-106E . . . . . . . . . . . . . . . . . . . . . . . . . . . . .233 122-10A6 . . . . . . . . . . . . . . . . . . . . . . . . . . . .279 122-10A6E . . . . . . . . . . . . . . . . . . . . . . . . . . .302 122-10AE . . . . . . . . . . . . . . . . . . . . . . . . . . . .233 122-10BE . . . . . . . . . . . . . . . . . . . . . . . . . . . .233 122-10G3 . . . . . . . . . . . . . . . . . . . . . . . . . . . .233 122-10H7 . . . . . . . . . . . . . . . . . . . . . . . . . . . .232 122-1111 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .270 122-1131 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .270 122-1211 . . . . . . . . . . . . . . . . . . . . .227, 247, 310 122-1212 . . . . . . . . . . . . . . . . . . . . .227, 247, 310 122-1213 . . . . . . . . . . . . . . . . . . . . . . . . .227, 247 122-1231 . . . . . . . . . . . . . . . . . . . . .227, 247, 310 122-1232 . . . . . . . . . . . . . . . . . . . . .227, 247, 310 122-1233 . . . . . . . . . . . . . . . . . . . . .227, 247, 310 122-1236 . . . . . . . . . . . . . . . . . . . . .227, 247, 310 122-1262 . . . . . . . . . . . . . . . . . . . . .227, 247, 310 122-1312 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .258 122-1313 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .258 122-1332 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .258 122-1333 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .258 122-1334 . . . . . . . . . . . . . . . . . . . . . . . . .257, 283 122-1334E . . . . . . . . . . . . . . . . . . . . . . . . . . . .301 122-1336 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .258 122-1362 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .258 122-1363 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .258 122-1363E . . . . . . . . . . . . . . . . . . . . . . . . . . . .301 122-1364 . . . . . . . . . . . . . . . . . . . . . . . . .257, 283 122-1364E . . . . . . . . . . . . . . . . . . . . . . . . . . . .301 122-1464 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .284 122-1534 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .282 122-1564 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .283 122-1564E . . . . . . . . . . . . . . . . . . . . . . . . . . . .302 122-1711 . . . . . . . . . . . . . . . . . . . . . . . . .251, 309 122-1712 . . . . . . . . . . . . . . . . . . . . . . . . .251, 309 122-1713 . . . . . . . . . . . . . . . . . . . . . . . . .251, 309 122-1731 . . . . . . . . . . . . . . . . . . . . . . . . .251, 309 122-1732 . . . . . . . . . . . . . . . . . . . . . . . . .251, 309 122-1733 . . . . . . . . . . . . . . . . . . . . . . . . .251, 309
Indices
722
Part Number Index

122-1762 . . . . . . . . . . . . . . . . . . . . . . . . .251, 309 122-1763 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .251 122-1811 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .271 122-1831 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .271 122-1932 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .248 122-1933 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .248 122-1934 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .248 122-1962 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .248 122-2012 . . . . . . . . . . . . . . . . . . . . . . . . .255, 310 122-2013 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .255 122-2032 . . . . . . . . . . . . . . . . . . . . . . . . .255, 310 122-2033 . . . . . . . . . . . . . . . . . . . . . . . . .255, 310 122-2212 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .256 122-2212E . . . . . . . . . . . . . . . . . . . . . . . . . . . .301 122-2231 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .256 122-2232 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .256 122-2312 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .254 122-2331 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .254 122-2332 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .254 122-2361 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .254 122-2362 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .254 122-2461 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .280 122-2462 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .280 122-2912 . . . . . . . . . . . . . . . . . . . . .230, 257, 310 122-2932 . . . . . . . . . . . . . . . . . . . . .230, 257, 310 122-2962 . . . . . . . . . . . . . . . . . . . . .230, 257, 310 122-3212 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .265 122-3232 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .265 122-3233 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .265 122-3262 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .265 122-3263 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .265 122-3812 . . . . . . . . . . . . . . . . . . . . .228, 250, 310 122-3831 . . . . . . . . . . . . . . . . . . . . . . . . .228, 250 122-3832 . . . . . . . . . . . . . . . . . . . . .228, 250, 310 122-3862 . . . . . . . . . . . . . . . . . . . . .228, 250, 310 122-4132 . . . . . . . . . . . . . . . . . . . . .224, 244, 309 122-4232 . . . . . . . . . . . . . . . . . . . . . . . . .227, 247 122-4711 . . . . . . . . . . . . . . . . . . . . . . . . .229, 253 122-4712 . . . . . . . . . . . . . . . . . . . . .229, 253, 310 122-4731 . . . . . . . . . . . . . . . . . . . . . . . . .229, 253 122-4732 . . . . . . . . . . . . . . . . . . . . .229, 253, 310 122-4762 . . . . . . . . . . . . . . . . . . . . .229, 253, 310 122-5011 . . . . . . . . . . . . . . . . . . . . . . . . .239, 308 122-5012 . . . . . . . . . . . . . . . . . . . . . . . . .239, 308 122-5013 . . . . . . . . . . . . . . . . . . . . . . . . .239, 308 122-501E . . . . . . . . . . . . . . . . . . . . . . . . . . . . .239 122-5022 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .239 122-502C . . . . . . . . . . . . . . . . . . . . . . . . . . . . .239 122-5031 . . . . . . . . . . . . . . . . . . . . . . . . .239, 308 122-5032 . . . . . . . . . . . . . . . . . . . . . . . . .239, 308 122-5032E . . . . . . . . . . . . . . . . . . . . . . . . . . . .301 122-5033 . . . . . . . . . . . . . . . . . . . . . . . . .240, 308 122-503E . . . . . . . . . . . . . . . . . . . . . . . . . . . . .240 122-5052 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .240 122-5061 . . . . . . . . . . . . . . . . . . . . . . . . .240, 308 122-5062 . . . . . . . . . . . . . . . . . . . . . . . . .240, 308 122-5063 . . . . . . . . . . . . . . . . . . . . . . . . .240, 308 122-506E . . . . . . . . . . . . . . . . . . . . . . . . . . . . .240 122-5511 . . . . . . . . . . . . . . . . . . . . .223, 243, 308 122-5512 . . . . . . . . . . . . . . . . . . . . .223, 243, 308 122-5513 . . . . . . . . . . . . . . . . . . . . .223, 243, 308 122-5516 . . . . . . . . . . . . . . . . . . . . . . . . .223, 243 122-5522 . . . . . . . . . . . . . . . . . . . . . . . . .223, 243 122-552A . . . . . . . . . . . . . . . . . . . . . . . .223, 243 122-5531 . . . . . . . . . . . . . . . . . . . . .224, 243, 308 122-5532 . . . . . . . . . . . . . . . . . . . . .224, 243, 308 122-5533 . . . . . . . . . . . . . . . . . . . . .224, 243, 308 122-5533E . . . . . . . . . . . . . . . . . . . . . . . . . . . .301 122-5536 . . . . . . . . . . . . . . . . . . . . .224, 243, 308 122-5552 . . . . . . . . . . . . . . . . . . . . . . . . .224, 244 122-5561 . . . . . . . . . . . . . . . . . . . . .224, 244, 308 122-5562 . . . . . . . . . . . . . . . . . . . . .224, 244, 308 122-5563 . . . . . . . . . . . . . . . . . . . . . . . . .224, 308 122-5631 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .281 122-5632 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .281 122-5633 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .281 122-5661 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .281 122-5711 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .271 122-5731 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .271 122-6112 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .297 122-6133 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .297 122-6312 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .297 122-6332 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .297 122-6412 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .297 122-6432 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .297 122-6462 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .297 122-6832 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .275 122-7011 . . . . . . . . . . . . . . . . . . . . . . . . .261, 309 122-7012 . . . . . . . . . . . . . . . . . . . . . . . . .261, 309 122-7013 . . . . . . . . . . . . . . . . . . . . . . . . .261, 309 122-7031 . . . . . . . . . . . . . . . . . . . . . . . . .261, 309 122-7032 . . . . . . . . . . . . . . . . . . . . . . . . .261, 309 122-7032E . . . . . . . . . . . . . . . . . . . . . . . . . . . .302 122-7033 . . . . . . . . . . . . . . . . . . . . . . . . .261, 309 122-7033E . . . . . . . . . . . . . . . . . . . . . . . . . . . .302 122-7061 . . . . . . . . . . . . . . . . . . . . . . . . .261, 309 122-7062 . . . . . . . . . . . . . . . . . . . . . . . . .261, 309 122-7063 . . . . . . . . . . . . . . . . . . . . . . . . .261, 309 122-7312 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .263 122-7313 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .263 122-7332 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .263 122-7333 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .263 122-7362 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .263 122-7363 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .263 122-7732 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .275 123-0112 . . . . . . . . . . . . . . . . . . . . .222, 237, 308 123-0131 . . . . . . . . . . . . . . . . . . . . .222, 237, 308 123-0132 . . . . . . . . . . . . . . . . . . . . .222, 237, 308 123-0162 . . . . . . . . . . . . . . . . . . . . .222, 237, 308 123-0212 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .255 123-0213 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .255 123-0231 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .255 123-0232 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .255 123-0233 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .255 123-0711 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .259 123-0712 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .259 123-0713 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .259 123-0731 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .259 123-0732 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .259 123-0732E . . . . . . . . . . . . . . . . . . . . . . . . . . . .301 123-0733 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .259 123-0733E . . . . . . . . . . . . . . . . . . . . . . . . . . . .301 123-0739 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .259 123-0753 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .259 123-0761 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .259 123-0762 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .259 123-0763 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .259 123-0763E . . . . . . . . . . . . . . . . . . . . . . . . . . . .301 123-1011 . . . . . . . . . . . . . . . . . . . . . . . . .233, 307 123-1012 . . . . . . . . . . . . . . . . . . . . . . . . .233, 307 123-1013 . . . . . . . . . . . . . . . . . . . . . . . . .233, 307 123-1014 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .233 123-1015 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .233 123-101E . . . . . . . . . . . . . . . . . . . . . . . . . . . . .233 123-1022 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .233 123-1026 . . . . . . . . . . . . . . . . . . . . . . . . .233, 307 123-1027 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .233 123-102F . . . . . . . . . . . . . . . . . . . . . . . . . . . . .233 123-1031 . . . . . . . . . . . . . . . . . . . . . . . . .233, 307 123-1032 . . . . . . . . . . . . . . . . . . . . . . . . .233, 307 123-1033 . . . . . . . . . . . . . . . . . . . . . . . . .233, 307 123-1033E . . . . . . . . . . . . . . . . . . . . . . . .233, 300 123-1034 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .233 123-1035 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .233 123-103B . . . . . . . . . . . . . . . . . . . . . . . . . . . .233 123-103G . . . . . . . . . . . . . . . . . . . . . . . . . . . .233 123-1052 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .233 123-1055 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .234 123-1056 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .233 123-105C . . . . . . . . . . . . . . . . . . . . . . . . . . . . .234 123-105F . . . . . . . . . . . . . . . . . . . . . . . . . . . . .234 123-1061 . . . . . . . . . . . . . . . . . . . . . . . . .234, 307 123-1062 . . . . . . . . . . . . . . . . . . . . . . . . .234, 307 123-1063 . . . . . . . . . . . . . . . . . . . . . . . . .234, 307 123-1064 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .234 123-1064E . . . . . . . . . . . . . . . . . . . . . . . . . . . .300 123-1065 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .234 123-1065E . . . . . . . . . . . . . . . . . . . . . . . . . . . .300 123-106B . . . . . . . . . . . . . . . . . . . . . . . . . . . .234 123-106E . . . . . . . . . . . . . . . . . . . . . . . . . . . . .234 123-106G . . . . . . . . . . . . . . . . . . . . . . . . . . . .234 123-1111 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .270 123-1131 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .270 123-1212 . . . . . . . . . . . . . . . . . . . . . . . . .227, 247 123-1213 . . . . . . . . . . . . . . . . . . . . . . . . .227, 247 123-1231 . . . . . . . . . . . . . . . . . . . . . . . . .227, 247 123-1232 . . . . . . . . . . . . . . . . . . . . .227, 247, 310 123-1233 . . . . . . . . . . . . . . . . . . . . .227, 247, 310 123-1236 . . . . . . . . . . . . . . . . . . . . .227, 247, 310 123-1262 . . . . . . . . . . . . . . . . . . . . .227, 247, 310 123-1312 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .258 123-1313 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .258 123-1332 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .258 123-1333 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .258
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123-1333E . . . . . . . . . . . . . . . . . . . . . . . . . . . .301 123-1334 . . . . . . . . . . . . . . . . . . . . . . . . .257, 283 123-1334E . . . . . . . . . . . . . . . . . . . . . . . . . . . .301 123-1362 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .258 123-1363 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .258 123-1364 . . . . . . . . . . . . . . . . . . . . . . . . .257, 283 123-1364E . . . . . . . . . . . . . . . . . . . . . . . . . . . .301 123-1464 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .284 123-1534 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .283 123-1564 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .283 123-1632 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .285 123-1711 . . . . . . . . . . . . . . . . . . . . . . . . .251, 309 123-1712 . . . . . . . . . . . . . . . . . . . . . . . . .251, 309 123-1713 . . . . . . . . . . . . . . . . . . . . . . . . .251, 309 123-1730 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .275 123-1731 . . . . . . . . . . . . . . . . . . . . . . . . .251, 309 123-1732 . . . . . . . . . . . . . . . . . . . . . . . . .252, 309 123-1732E . . . . . . . . . . . . . . . . . . . . . . . . . . . .301 123-1733 . . . . . . . . . . . . . . . . . . . . . . . . .252, 309 123-1762 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .252 123-1763 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .252 123-1811 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .271 123-1831 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .271 123-1861 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .271 123-1932 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .248 123-1933 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .248 123-1934 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .248 123-1962 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .248 123-2032 . . . . . . . . . . . . . . . . . . . . . . . . .255, 310 123-2033 . . . . . . . . . . . . . . . . . . . . . . . . .255, 310 123-2212 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .256 123-2231 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .256 123-2232 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .256 123-2312 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .254 123-2331 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .254 123-2332 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .254 123-2361 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .254 123-2362 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .254 123-2461 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .280 123-2462 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .280 123-2912 . . . . . . . . . . . . . . . . . . . . .230, 257, 310 123-2932 . . . . . . . . . . . . . . . . . . . . .230, 257, 310 123-3212 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .265 123-3214 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .265 123-3223 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .265 123-3232 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .265 123-3232E . . . . . . . . . . . . . . . . . . . . . . . . . . . .302 123-3233 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .265 123-3234 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .265 123-3253 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .265 123-3262 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .265 123-3263 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .265 123-3264 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .265 123-3812 . . . . . . . . . . . . . . . . . . . . . . . . .228, 250 123-3832 . . . . . . . . . . . . . . . . . . . . .228, 250, 310 123-3862 . . . . . . . . . . . . . . . . . . . . . . . . .228, 250 123-4711 . . . . . . . . . . . . . . . . . . . . . . . . .229, 253 123-4712 . . . . . . . . . . . . . . . . . . . . .229, 253, 310 123-4731 . . . . . . . . . . . . . . . . . . . . . . . . .229, 253 123-4732 . . . . . . . . . . . . . . . . . . . . .229, 253, 310 123-5002 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .240 123-5011 . . . . . . . . . . . . . . . . . . . . . . . . .240, 308 123-5012 . . . . . . . . . . . . . . . . . . . . . . . . .240, 308 123-5012E . . . . . . . . . . . . . . . . . . . . . . . . . . . .301 123-5013 . . . . . . . . . . . . . . . . . . . . . . . . .240, 308 123-501E . . . . . . . . . . . . . . . . . . . . . . . . . . . . .240 123-5022 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .240 123-5026 . . . . . . . . . . . . . . . . . . . . . . . . .240, 308 123-502D . . . . . . . . . . . . . . . . . . . . . . . . . . . .240 123-502F . . . . . . . . . . . . . . . . . . . . . . . . . . . . .240 123-5031 . . . . . . . . . . . . . . . . . . . . . . . . .240, 308 123-5032 . . . . . . . . . . . . . . . . . . . . . . . . .240, 308 123-5032E . . . . . . . . . . . . . . . . . . . . . . . . . . . .301 123-5033 . . . . . . . . . . . . . . . . . . . . . . . . .240, 308 123-503B . . . . . . . . . . . . . . . . . . . . . . . . . . . .240 123-503E . . . . . . . . . . . . . . . . . . . . . . . . . . . . .240 123-5052 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .240 123-5053 . . . . . . . . . . . . . . . . . . . . . . . . .240, 308 123-5056 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .240 123-505D . . . . . . . . . . . . . . . . . . . . . . . . . . . .240 123-505f . . . . . . . . . . . . . . . . . . . . . . . . . . . . .240 123-5061 . . . . . . . . . . . . . . . . . . . . . . . . .240, 308 123-5062 . . . . . . . . . . . . . . . . . . . . . . . . .240, 308 123-5063 . . . . . . . . . . . . . . . . . . . . . . . . .240, 308 123-5063E . . . . . . . . . . . . . . . . . . . . . . . . . . . .301 123-506E . . . . . . . . . . . . . . . . . . . . . . . . . . . . .240 123-5511 . . . . . . . . . . . . . . . . . . . . .224, 244, 309 123-5512 . . . . . . . . . . . . . . . . . . . . .224, 244, 309 123-5513 . . . . . . . . . . . . . . . . . . . . .224, 244, 309 123-5526 . . . . . . . . . . . . . . . . . . . . .224, 244, 309 123-5531 . . . . . . . . . . . . . . . . . . . . .224, 244, 309 123-5532 . . . . . . . . . . . . . . . . . . . . .224, 244, 309 123-5532E . . . . . . . . . . . . . . . . . . . . . . . . . . . .301 123-5533 . . . . . . . . . . . . . . . . . . . . .224, 244, 309 123-5536 . . . . . . . . . . . . . . . . . . . . .224, 244, 309 123-5552 . . . . . . . . . . . . . . . . . . . . . . . . .224, 244 123-5553 . . . . . . . . . . . . . . . . . . . . . . . . .224, 244 123-5561 . . . . . . . . . . . . . . . . . . . . .224, 244, 309 123-5562 . . . . . . . . . . . . . . . . . . . . .224, 244, 309 123-5563 . . . . . . . . . . . . . . . . . . . . .224, 244, 309 123-5566 . . . . . . . . . . . . . . . . . . . . . . . . .224, 244 123-5631 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .281 123-5632 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .281 123-5633 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .281 123-5711 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .271 123-5731 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .271 123-6133 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .297 123-6332 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .297 123-6412 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .297 123-6432 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .297 123-6462 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .297 123-7011 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .262 123-7012 . . . . . . . . . . . . . . . . . . . . . . . . .262, 309 123-7013 . . . . . . . . . . . . . . . . . . . . . . . . .262, 309 123-7031 . . . . . . . . . . . . . . . . . . . . . . . . .262, 309 123-7032 . . . . . . . . . . . . . . . . . . . . . . . . .262, 309 123-7032E . . . . . . . . . . . . . . . . . . . . . . . . . . . .302 123-7033 . . . . . . . . . . . . . . . . . . . . . . . . .262, 309 123-7033E . . . . . . . . . . . . . . . . . . . . . . . . . . . .302 123-7062 . . . . . . . . . . . . . . . . . . . . . . . . .262, 309 123-7063 . . . . . . . . . . . . . . . . . . . . . . . . .262, 309 123-7063E . . . . . . . . . . . . . . . . . . . . . . . . . . . .302 123-7312 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .263 123-7313 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .263 123-7314 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .263 123-7332 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .263 123-7333 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .263 123-7334 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .263 123-7354 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .263 123-7362 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .263 123-7363 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .263 123-7364 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .263 123-7722 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .275 123-7732 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .275 123-7732E . . . . . . . . . . . . . . . . . . . . . . . . . . . .302 123-9134 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .272 123-9234 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .272 124-0034 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .285 124-0732 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .259 124-0737 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .259 124-1005 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .234 124-1012 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .234 124-1014 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .234 124-1032 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .234 124-1034 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .234 124-1037 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .234 124-1062 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .234 124-1232 . . . . . . . . . . . . . . . . . . . . . . . . .227, 247 124-1334 . . . . . . . . . . . . . . . . . . . . . . . . .257, 283 124-1374 . . . . . . . . . . . . . . . . . . . . . . . . .257, 283 124-1474 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .284 124-14A4 . . . . . . . . . . . . . . . . . . . . . . . . . . . .284 124-1534 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .283 124-1574 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .283 124-1632 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .285 124-1712 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .252 124-1730 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .275 124-1732 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .252 124-2032 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .255 124-2814 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .277 124-3212 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .266 124-3232 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .266 124-5012 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .240 124-5032 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .240 124-5035 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .240 124-5037 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .240 124-6837 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .275 124-7012 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .262 124-7032 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .262 124-7062 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .262 124-7304 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .263 125-0034 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .285 125-0034E . . . . . . . . . . . . . . . . . . . . . . . . . . . .302 125-0212 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .255
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724
Part Number Index

125-0232 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .255 125-0711 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .259 125-0712 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .259 125-0717 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .259 125-0731 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .259 125-0732 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .259 125-0732E . . . . . . . . . . . . . . . . . . . . . . . . . . . .301 125-0733 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .259 125-0737 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .259 125-0739 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .259 125-0762 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .259 125-0763 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .259 125-1005 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .234 125-100B . . . . . . . . . . . . . . . . . . . . . . . . . . . .234 125-1011 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .234 125-1011E . . . . . . . . . . . . . . . . . . . . . . . . . . . .300 125-1012 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .234 125-1012E . . . . . . . . . . . . . . . . . . . . . . . . . . . .300 125-1014 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .234 125-1015 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .234 125-1017 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .234 125-101J . . . . . . . . . . . . . . . . . . . . . . . . . . . . .234 125-101K . . . . . . . . . . . . . . . . . . . . . . . . . . . . .234 125-1025 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .234 125-102J . . . . . . . . . . . . . . . . . . . . . . . . . . . . .234 125-1032 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .235 125-1034 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .235 125-1034E . . . . . . . . . . . . . . . . . . . . . . . . . . . .300 125-1035 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .235 125-1035E . . . . . . . . . . . . . . . . . . . . . . . . . . . .300 125-1037 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .234 125-1039 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .234 125-103B . . . . . . . . . . . . . . . . . . . . . . . . . . . .235 125-103D . . . . . . . . . . . . . . . . . . . . . . . . . . . .235 125-103J . . . . . . . . . . . . . . . . . . . . . . . . . . . . .235 125-103K . . . . . . . . . . . . . . . . . . . . . . . . . . . . .234 125-1055 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .235 125-1062 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .235 125-1064 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .235 125-1065 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .235 125-106J . . . . . . . . . . . . . . . . . . . . . . . . . . . . .235 125-10B5 . . . . . . . . . . . . . . . . . . . . . . . . . . . .235 125-10H5 . . . . . . . . . . . . . . . . . . . . . . . . . . . .234 125-10HB . . . . . . . . . . . . . . . . . . . . . . . . . . . .234 125-1212 . . . . . . . . . . . . . . . . . . . . . . . . .227, 247 125-1232 . . . . . . . . . . . . . . . . . . . . . . . . .227, 247 125-1312 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .258 125-1313 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .258 125-1332 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .258 125-1333 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .258 125-1334 . . . . . . . . . . . . . . . . . . . . . . . . .257, 283 125-1334E . . . . . . . . . . . . . . . . . . . . . . . . . . . .301 125-1363 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .258 125-1364 . . . . . . . . . . . . . . . . . . . . . . . . .257, 283 125-1374 . . . . . . . . . . . . . . . . . . . . . . . . .257, 283 125-1374E . . . . . . . . . . . . . . . . . . . . . . . . . . . .301 125-14A4 . . . . . . . . . . . . . . . . . . . . . . . . . . . .284 125-1704 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .252 125-1710 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .275 125-1711 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .252 125-1712 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .252 125-1713 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .252 125-1717 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .252 125-1719 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .252 125-1730 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .275 125-1731 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .252 125-1732 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .252 125-1733 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .252 125-1737 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .252 125-1762 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .252 125-1763 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .252 125-1912 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .248 125-1932 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .248 125-1937 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .248 125-1962 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .248 125-1963 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .248 125-2012 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .255 125-2032 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .255 125-2037 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .255 125-2212 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .256 125-2232 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .256 125-2237 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .256 125-2312 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .254 125-2332 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .254 125-2814 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .277 125-2834 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .277 125-3212 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .266 125-3217 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .266 125-3231 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .266 125-3232 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .266 125-3233 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .266 125-3237 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .266 125-3262 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .266 125-32H2 . . . . . . . . . . . . . . . . . . . . . . . . . . . .266 125-3812 . . . . . . . . . . . . . . . . . . . . . . . . .228, 250 125-3832 . . . . . . . . . . . . . . . . . . . . . . . . .228, 250 125-3837 . . . . . . . . . . . . . . . . . . . . . . . . .228, 250 125-5012 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .241 125-5014 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .241 125-5015 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .241 125-5017 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .241 125-501J . . . . . . . . . . . . . . . . . . . . . . . . . . . . .241 125-501K . . . . . . . . . . . . . . . . . . . . . . . . . . . . .241 125-5023 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .241 125-5025 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .241 125-5032 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .241 125-5032E . . . . . . . . . . . . . . . . . . . . . . . . . . . .301 125-5034 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .241 125-5035 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .241 125-5037 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .241 125-503B . . . . . . . . . . . . . . . . . . . . . . . . . . . .241 125-503D . . . . . . . . . . . . . . . . . . . . . . . . . . . .241 125-503J . . . . . . . . . . . . . . . . . . . . . . . . . . . . .241 125-503K . . . . . . . . . . . . . . . . . . . . . . . . . . . . .241 125-505J . . . . . . . . . . . . . . . . . . . . . . . . . . . . .241 125-5062 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .241 125-5062E . . . . . . . . . . . . . . . . . . . . . . . . . . . .301 125-5064 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .241 125-5065 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .241 125-50H3 . . . . . . . . . . . . . . . . . . . . . . . . . . . .240 125-50H5 . . . . . . . . . . . . . . . . . . . . . . . . . . . .240 125-50HB . . . . . . . . . . . . . . . . . . . . . . . . . . . .240 125-5512 . . . . . . . . . . . . . . . . . . . . . . . . .224, 244 125-5517 . . . . . . . . . . . . . . . . . . . . . . . . .224, 244 125-551J . . . . . . . . . . . . . . . . . . . . . . . . .224, 244 125-5532 . . . . . . . . . . . . . . . . . . . . . . . . .224, 244 125-5537 . . . . . . . . . . . . . . . . . . . . . . . . .224, 244 125-553J . . . . . . . . . . . . . . . . . . . . . . . . .224, 244 125-5632 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .281 125-6837 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .275 125-7012 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .262 125-7017 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .262 125-7031 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .262 125-7032 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .262 125-7032E . . . . . . . . . . . . . . . . . . . . . . . . . . . .302 125-7037 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .262 125-7062 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .262 125-7304 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .263 125-7312 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .263 125-7314 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .263 125-7332 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .263 125-7333 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .263 125-7334 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .263 125-7362 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .263 125-7363 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .263 125-7732 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .275 125-9134 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .272 125-9234 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .272 126-1013 . . . . . . . . . . . . . . . . . . . . . . . . .232, 665 126-1713 . . . . . . . . . . . . . . . . . . . . . . . . .251, 665 126-5012 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .239 126-5013 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .239 126-7012 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .261 126-7013 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .261 127-0112 . . . . . . . . . . . . . . . . . . . . . . . . .221, 237 127-0113 . . . . . . . . . . . . . . . . . . . . . . . . .221, 237 127-0113E . . . . . . . . . . . . . . . . . . . . . . . . . . . .300 127-0122 . . . . . . . . . . . . . . . . . . . . . . . . .221, 237 127-0123 . . . . . . . . . . . . . . . . . . . . . . . . .221, 237 127-0722 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .259 127-0723 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .259 127-100A . . . . . . . . . . . . . . . . . . . . . . . . . . . .232 127-1012 . . . . . . . . . . . . . . . . . . . . . . . . .232, 665 127-1012E . . . . . . . . . . . . . . . . . . . . . . . . . . . .300 127-1013 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .232 127-1013E . . . . . . . . . . . . . . . . . . . . . . . . . . . .300 127-101A . . . . . . . . . . . . . . . . . . . . . . . . . . . .232 127-1022 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .232 127-1023 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .232 127-1043 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .232 127-1043E . . . . . . . . . . . . . . . . . . . . . . . . . . . .300 127-1046 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .232 127-1712 . . . . . . . . . . . . . . . . . . . . . . . . .251, 665 127-1713 . . . . . . . . . . . . . . . . . . . . . . . . .251, 665
Indices
725
Part Number Index

127-1722 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .251 127-1723 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .251 127-2222 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .256 127-500A . . . . . . . . . . . . . . . . . . . . . . . . . . . .239 127-5012 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .239 127-5013 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .239 127-501E . . . . . . . . . . . . . . . . . . . . . . . . . . . . .239 127-501N . . . . . . . . . . . . . . . . . . . . . . . . . . . .239 127-5022 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .239 127-5023 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .239 127-5046 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .239 127-7012 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .261 127-7012E . . . . . . . . . . . . . . . . . . . . . . . . . . . .302 127-7013 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .261 127-7013E . . . . . . . . . . . . . . . . . . . . . . . . . . . .302 127-7022 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .261 127-7023 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .261 128-0112 . . . . . . . . . . . . . . . . . . . . .221, 237, 308 128-0122 . . . . . . . . . . . . . . . . . . . . .221, 237, 308 128-0722 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .259 128-1012 . . . . . . . . . . . . . . . . . . . . . . . . .232, 307 128-1022 . . . . . . . . . . . . . . . . . . . . . . . . .232, 307 128-1052 . . . . . . . . . . . . . . . . . . . . . . . . .232, 307 128-1056 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .279 128-10H7 . . . . . . . . . . . . . . . . . . . . . . . . . . . .232 128-1212 . . . . . . . . . . . . . . . . . . . . .227, 247, 310 128-1222 . . . . . . . . . . . . . . . . . . . . .227, 247, 310 128-1324 . . . . . . . . . . . . . . . . . . . . . . . . .257, 283 128-1721 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .251 128-3222 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .265 128-3812 . . . . . . . . . . . . . . . . . . . . . . . . .228, 250 128-3822 . . . . . . . . . . . . . . . . . . . . .228, 250, 310 128-5012 . . . . . . . . . . . . . . . . . . . . . . . . .239, 308 128-5021 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .239 128-5022 . . . . . . . . . . . . . . . . . . . . . . . . .239, 308 128-5051 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .239 128-5052 . . . . . . . . . . . . . . . . . . . . . . . . .239, 308 128-50H7 . . . . . . . . . . . . . . . . . . . . . . . . . . . .239 128-5512 . . . . . . . . . . . . . . . . . . . . .223, 243, 308 128-5522 . . . . . . . . . . . . . . . . . . . . .223, 243, 308 128-5552 . . . . . . . . . . . . . . . . . . . . .223, 243, 308 128-7022 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .261 128-7032 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .261 128-7052 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .261 128-7053 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .261 128-7062 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .261 128-7323 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .263 128-8122 . . . . . . . . . . . . . . . . . . . . . . . . .131, 273 128-8522 . . . . . . . . . . . . . . . . . . . . . . . . .131, 273 128-8722 . . . . . . . . . . . . . . . . . . . . . . . . .131, 273 12A-1015 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .232 12A-1025 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .232 12A-5015 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .239 14-0036-050TMR . . . . . . . . . . . . . . . . . . . . . .171 14-0216-016TMR . . . . . . . . . . . . . . . . . . . . . .171 14-0739-016TMR . . . . . . . . . . . . . . . . . . . . . .171 14-0857-016TMR . . . . . . . . . . . . . . . . . . . . . .171 14-2087-016TMR . . . . . . . . . . . . . . . . . . . . . .171 14-2261-016TMR . . . . . . . . . . . . . . . . . . . . . .171 14-3123-016TMR . . . . . . . . . . . . . . . . . . . . . .171 14-3124-016TMR . . . . . . . . . . . . . . . . . . . . . .171 14-3137-200TMR . . . . . . . . . . . . . . . . . . . . . .171 14-3195-053TMR . . . . . . . . . . . . . . . . . . . . . .170 14-3197-100TMR . . . . . . . . . . . . . . . . . . . . . .171 14-3198-100TMR . . . . . . . . . . . . . . . . . . . . . .171 14-3914-016TMR . . . . . . . . . . . . . . . . . . . . . .171 14-3965-016TMR . . . . . . . . . . . . . . . . . . . . . .171 14-3979-016TMR . . . . . . . . . . . . . . . . . . . . . .171 14-5186-153TMR . . . . . . . . . . . . . . . . . . . . . .170 14-5681-102 . . . . . . . . . . . . . . . . . . . . . . . . . .170 1400-0015 . . . . . . . . . . . . . . . . . . . . . . . . . . . .184 145-1001 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .278 145-1011 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .293 145-1012 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .293 145-1017 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .293 145-1031 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .293 145-1032 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .293 145-1034 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .293 145-1034E . . . . . . . . . . . . . . . . . . . . . . . . . . . .302 145-1035 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .293 145-1037 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .293 145-1334 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .293 145-2814 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .293 145-5032 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .293 145-7012 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .293 145-7032 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .293 1520-0401 . . . . . . . . . . . . . . . . . . . . . . . . . . . .657 1530-2163 . . . . . . . . . . . . . . . . . . . . . . . . . . . .168 1530-2167 . . . . . . . . . . . . . . . . . . . . . . . . . . . .168 1535-4046 . . . . . . . . . . . . . . . . . . . . . . . . . . . .487 1535-4048 . . . . . . . . . . . . . . . . . . . . . . . .495497 1535-4545 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .92 1535-4546 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .92 1535-4857 . . . . . . . . . . . . . . . . . . . . . . . . . . . .466 1535-4860 . . . . . . . . . . . . . . . . . . . .452, 470, 496 1535-4861 . . . . . . . . . . . . . . . . . . . . . . . . . . . .496 1535-4878 . . . . . . . . . . . . . . . . . . . . . . . . . . . .697 1535-4900 . . . . . . . . . . . . . . . . . . . . . . . .495496 1535-4952 . . . . . . . . . . . . . . . . . . . . . . . . . . . .168 1535-4954 . . . . . . . . . . . . . . . . . . . . . . . . . . . .168 1535-4970 . . . . . . . . . . . . . . . . . . . .484, 678, 687 1535-5082 . . . . . . . . . . . . . . . . . . . . . . . . . . . .487 1535-5083 . . . . . . . . . . . . . . . . . . . . . . . . . . . .487 1535-5084 . . . . . . . . . . . . . . . . . . . . . . . . . . . .487 160-1010-1 . . . . . . . . . . . . . . . . . . . . . . . . . . .311 160-1010-10 . . . . . . . . . . . . . . . . . . . . . . . . . .311 160-1010-5 . . . . . . . . . . . . . . . . . . . . . . . . . . .311 160-2200-10 . . . . . . . . . . . . . . . . . . . . . . . . . .313 160-2200-5 . . . . . . . . . . . . . . . . . . . . . . . . . . .313 160-2205-1 . . . . . . . . . . . . . . . . . . . . . . . . . . .311 160-2205-10 . . . . . . . . . . . . . . . . . . . . . . . . . .311 160-2205-5 . . . . . . . . . . . . . . . . . . . . . . . . . . .311 160-2250-10 . . . . . . . . . . . . . . . . . . . . . . . . . .313 160-2250-5 . . . . . . . . . . . . . . . . . . . . . . . . . . .313 160-2255-1 . . . . . . . . . . . . . . . . . . . . . . . . . . .311 160-2255-10 . . . . . . . . . . . . . . . . . . . . . . . . . .311 160-2255-30 . . . . . . . . . . . . . . . . . . . . . . . . . .311 160-2255-5 . . . . . . . . . . . . . . . . . . . . . . .179, 311 160-2320-10 . . . . . . . . . . . . . . . . . . . . . . . . . .313 160-2320-5 . . . . . . . . . . . . . . . . . . . . . . . . . . .313 160-2325-1 . . . . . . . . . . . . . . . . . . . . . . . . . . .311 160-2325-10 . . . . . . . . . . . . . . . . . . . . . . . . . .311 160-2325-30 . . . . . . . . . . . . . . . . . . . . . . . . . .311 160-2325-5 . . . . . . . . . . . . . . . . . . .179, 190, 311 160-2450-10 . . . . . . . . . . . . . . . . . . . . . . . . . .313 160-2450-5 . . . . . . . . . . . . . . . . . . . . . . . . . . .313 160-2455-1 . . . . . . . . . . . . . . . . . . . . . . . . . . .311 160-2455-10 . . . . . . . . . . . . . . . . . . . . . . . . . .311 160-2455-5 . . . . . . . . . . . . . . . . . . . . . . . . . . .311 160-2530-10 . . . . . . . . . . . . . . . . . . . . . .165, 313 160-2530-5 . . . . . . . . . . . . . . . . . . . . . . . . . . .313 160-2535-1 . . . . . . . . . . . . . . . . . . . . . . . . . . .311 160-2535-10 . . . . . . . . . . . . . . . . . . . . . . . . . .311 160-2535-30 . . . . . . . . . . . . . . . . . . . . . . . . . .311 160-2535-5 . . . . . . . . . . . . . . . . . . . . . . .179, 311 160-2610-10 . . . . . . . . . . . . . . . . . . . . . . . . . .313 160-2610-5 . . . . . . . . . . . . . . . . . . . . . . . . . . .313 160-2615-1 . . . . . . . . . . . . . . . . . . . . . . . . . . .311 160-2615-10 . . . . . . . . . . . . . . . . . . . . . . . . . .311 160-2615-5 . . . . . . . . . . . . . . . . . . . . . . . . . . .311 160-2634-10 . . . . . . . . . . . . . . . . . . . . . . . . . .313 160-2634-5 . . . . . . . . . . . . . . . . . . . . . . . . . . .313 160-2635-1 . . . . . . . . . . . . . . . . . . . . . . . . . . .311 160-2635-10 . . . . . . . . . . . . . . . . . . . . . . . . . .311 160-2635-5 . . . . . . . . . . . . . . . . . . . . . . . . . . .311 160-2644-10 . . . . . . . . . . . . . . . . . . . . . . . . . .313 160-2644-5 . . . . . . . . . . . . . . . . . . . . . . . . . . .662 160-2650-10 . . . . . . . . . . . . . . . . . . . . . . . . . .313 160-2650-5 . . . . . . . . . . . . . . . . . . . . . . .313, 662 160-2655-1 . . . . . . . . . . . . . . . . . . . . . . . . . . .311 160-2655-10 . . . . . . . . . . . . . . . . . . . . . . . . . .311 160-2655-5 . . . . . . . . . . . . . . . . . . . . . . . . . . .311 160-2660-10 . . . . . . . . . . . . . . . . . . . . . . . . . .313 160-2660-5 . . . . . . . . . . . . . . . . . . . . . . .313, 662 160-2815-5 . . . . . . . . . . . . . . . . . . . . . . . . . . .312 160-2825-5 . . . . . . . . . . . . . . . . . . . . . . . . . . .312 160-2835-5 . . . . . . . . . . . . . . . . . . . . . . . . . . .312 160-2845-10 . . . . . . . . . . . . . . . . . . . . . . . . . .312 160-2845-5 . . . . . . . . . . . . . . . . . . . . . . . . . . .312 160-2855-10 . . . . . . . . . . . . . . . . . . . . . . . . . .312 160-2855-5 . . . . . . . . . . . . . . . . . . . . . . . . . . .312 160-2865-10 . . . . . . . . . . . . . . . . . . . . . . . . . .312 160-2865-5 . . . . . . . . . . . . . . . . . . . . . . . . . . .312 160-4535-5 . . . . . . . . . . . . . . . . . . . . . . . . . . .312 18587-60560 . . . . . . . . . . . . . . . . . . . . . . . . . .173 18596-40015 . . . . . . . . . . . . . . . . . . . . . . . . . .173 18710-20119 . . . . . . . . . . . . .182184, 192193 18710-60170 . . . . . . . . . . . . . . .98, 182183, 192 18711-60060 . . . . . . . . . . . . . . . . . . .98, 185, 194 18713-60040 . . . . . . . . . . . . . . . . . . .98, 187, 194 18713-60050 . . . . . . . . . . . . . . . . . . . . . . . . . .187 18740-20800 . . . . . . . . . . . . . . . . . . . . . .176, 188 18740-20880 . . . . . . . . . . . . . . . . . . . . . .176, 188 18740-20885 . . . . . . . . . . . . . . . . . .176, 188189
Indices
726
Part Number Index

18740-20950 . . . . . . . . . . . . . . . . . . . . . .185, 194 18740-20960 . . . . . . . . . . . . . . . . . . . . . .185, 194 18740-60830 . . . . . . . . . . . . . . . . . .175, 188, 191 18740-60835 . . . . . . . . . .175, 177, 180, 188, 191 18740-60840 . . . . . . . . . . . . . . . . . . . . . .157, 189 18740-80190 . . . . . . . . . . . . . . . . . . . . . . . . . .157 18740-80200 . . . . . . . . . . . . . . . . . . . . . . . . . .157 18740-80220 . . . . . . . . . . . . . . . . . . . . . . . . . .157 18789-60060 . . . . . . . . . . . . . . . . . . .98, 185, 193 18789-80070 . . . . . . . . . . . . . . . . . . . . . .183, 193 188-0110 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .122 188-0120 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .122 188-0150 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .122 188-0156 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .122 188-0160 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .122 188-0310 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .121 188-0320 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .121 188-0350 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .121 188-0356 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .121 188-0360 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .121 188-0510 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .122 188-0520 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .122 188-0550 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .122 188-0556 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .122 188-0560 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .122 188-0610 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .123 188-0620 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .123 188-0650 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .123 188-0656 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .123 188-0660 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .123 188-0710 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .123 188-0720 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .123 188-0750 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .123 188-0756 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .123 188-0760 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .123 188-1010 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .124 188-1020 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .124 188-1050 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .124 188-1056 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .124 188-1060 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .124 188-1310 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .120 188-1320 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .120 188-1350 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .120 188-1356 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .120 188-1360 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .120 188-1510 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .125 188-1520 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .125 188-1550 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .125 188-1556 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .125 188-1560 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .125 188-1610 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .124 188-1620 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .124 188-1650 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .124 188-1656 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .124 188-1660 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .124 188-2110 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .127 188-2120 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .127 188-2150 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .127 188-2156 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .127 188-2160 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .127 188-2210 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .127 188-2220 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .127 188-2250 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .127 188-2256 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .127 188-2260 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .127 188-2310 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .128 188-2320 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .128 188-2350 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .128 188-2356 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .128 188-2360 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .128 188-2410 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .128 188-2420 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .128 188-2450 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .128 188-2456 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .128 188-2460 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .128 188-2920 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .130 188-2946 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .130 189-0102 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .129 189-0302 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .129 189-0502 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .129 189-0602 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .129 189-0702 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .129 189-1002 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .129 189-1302 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .129 189-1502 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .129 189-1602 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .129 189-2102 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .129 189-2202 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .129 189-2302 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .129 189-2402 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .129 18900-20250 . . . . . . . . . . . . . . . . . . . . . . . . . .168 18900-20280 . . . . . . . . . . . . . . . . . . . . . . . . . .168 18900-20281 . . . . . . . . . . . . . . . . . . . . . . . . . .168 18900-20300 . . . . . . . . . . . . . . . . . . . . . . . . . .168 18900-20850 . . . . . . . . . . . . . . . . . . . . . . . . . .167 18900-60640 . . . . . . . . . . . . . . . . . . . . . . . . . . .99 18900-80255 . . . . . . . . . . . . . . . . . . . . . . . . . .168 190-0131 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .662 190-0231 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .662 190-0331 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .662 190-0431 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .662 19091-20050 . . . . . . . . . . . . . . . . . . . . . . . . . .313 19091-21050 . . . . . . . . . . . . . . . . . . . . . . . . . .313 19091-60010 . . . . . . . . . . . . . . . . . . . . . . . . . .294 19091-60010E . . . . . . . . . . . . . . . . . . . . . . . . .304 19091-60312 . . . . . . . . . . . . . . . . . . . . . . . . . .235 19091-60620E . . . . . . . . . . . . . . . . . . . . . . . . .311 19091-63000 . . . . . . . . . . . . . . . . . . .45, 178, 190 19091-80060 . . . . . . . . . . . . . . . . . . . . . . . . . .306 19091A-002 . . . . . . . . . . . . . . . . . . . . . . . . . . .269 19091A-005 . . . . . . . . . . . . . . . . . . . . . . . . . . .270 19091A-012 . . . . . . . . . . . . . . . . . . . . . . . . . . .270 19091A-015 . . . . . . . . . . . . . . . . . . . . . . . . . . .270 19091A-101 . . . . . . . . . . . . . . . . . . . . . . . . . . .269 19091A-102 . . . . . . . . . . . . . . . . . . . . . . . . . . .269 19091A-102E . . . . . . . . . . . . . . . . . . . . . . . . .306 19091A-105 . . . . . . . . . . . . . . . . . . . . . . . . . . .270 19091A-112 . . . . . . . . . . . . . . . . . . . . . . . . . . .270 19091A-115 . . . . . . . . . . . . . . . . . . . . . . . . . . .270 19091B-002 . . . . . . . . . . . . . . . . . . . . . . . . . .270 19091B-005 . . . . . . . . . . . . . . . . . . . . . . . . . .270 19091B-012 . . . . . . . . . . . . . . . . . . . . . . . . . .270 19091B-012E . . . . . . . . . . . . . . . . . . . . . . . . .306 19091B-015 . . . . . . . . . . . . . . . . . . . . . . . . . .270 19091B-101 . . . . . . . . . . . . . . . . . . . . . . . . . .270 19091B-102 . . . . . . . . . . . . . . . . . . . . . . . . . .270 19091B-102E . . . . . . . . . . . . . . . . . . . . . . . . .306 19091B-105 . . . . . . . . . . . . . . . . . . . . . . . . . .270 19091B-112 . . . . . . . . . . . . . . . . . . . . . . . . . .270 19091B-115 . . . . . . . . . . . . . . . . . . . . . . . . . .270 19091F-102 . . . . . . . . . . . . . . . . . . . . . . . . . . .266 19091F-105 . . . . . . . . . . . . . . . . . . . . . . . . . . .266 19091F-105E . . . . . . . . . . . . . . . . . . . . . . . . . .305 19091F-112 . . . . . . . . . . . . . . . . . . . . . . . . . . .266 19091F-112E . . . . . . . . . . . . . . . . . . . . . . . . . .305 19091F-115 . . . . . . . . . . . . . . . . . . . . . . . . . . .266 19091F-115E . . . . . . . . . . . . . . . . . . . . . . . . . .305 19091F-411 . . . . . . . . . . . . . . . . . . . . . . . . . . .266 19091F-413 . . . . . . . . . . . . . . . . . . . . . . . . . . .266 19091F-431 . . . . . . . . . . . . . . . . . . . . . . . . . . .266 19091F-433 . . . . . . . . . . . . . . . . . . . . . . . . . . .266 19091F-433E . . . . . . . . . . . . . . . . . . . . . . . . . .305 19091G-013 . . . . . . . . . . . . . . . . . . . . . . . . . .249 19091G-113 . . . . . . . . . . . . . . . . . . . . . . . . . .249 19091G-131 . . . . . . . . . . . . . . . . . . . . . . . . . .249 19091G-133 . . . . . . . . . . . . . . . . . . . . . . . . . .249 19091G-136 . . . . . . . . . . . . . . . . . . . . . . . . . .249 19091G-213 . . . . . . . . . . . . . . . . . . . . . . . . . .249 19091G-B113 . . . . . . . . . . . . . . . . . . . . . . . . .268 19091G-B133 . . . . . . . . . . . . . . . . . . . . . . . . .268 19091G-B213 . . . . . . . . . . . . . . . . . . . . . . . . .268 19091G-B233 . . . . . . . . . . . . . . . . . . . . . . . . .268 19091J-002 . . . . . . . . . . . . . . . . . . . . . . . . . . .242 19091J-005 . . . . . . . . . . . . . . . . . . . . . . . . . . .242 19091J-012 . . . . . . . . . . . . . . . . . . . . . . . . . . .242 19091J-015 . . . . . . . . . . . . . . . . . . . . . . . . . . .242 19091J-101 . . . . . . . . . . . . . . . . . . . . . . . . . . .242 19091J-102 . . . . . . . . . . . . . . . . . . . . . . . . . . .242 19091J-102E . . . . . . . . . . . . . . . . . . . . . . . . . .304 19091J-105 . . . . . . . . . . . . . . . . . . . . . . . . . . .242 19091J-112 . . . . . . . . . . . . . . . . . . . . . . . . . . .242 19091J-112E . . . . . . . . . . . . . . . . . . . . . . . . . .304 19091J-113 . . . . . . . . . . . . . . . . . . . . . . . . . . .242 19091J-115E . . . . . . . . . . . . . . . . . . . . . .242, 304 19091J-202 . . . . . . . . . . . . . . . . . . . . . . . . . . .242 19091J-205 . . . . . . . . . . . . . . . . . . . . . . . . . . .242 19091J-211 . . . . . . . . . . . . . . . . . . . . . . . . . . .242 19091J-212 . . . . . . . . . . . . . . . . . . . . . . . . . . .242 19091J-213 . . . . . . . . . . . . . . . . . . . . . . . . . . .242 19091J-213E . . . . . . . . . . . . . . . . . . . . . . . . . .242 19091J-215 . . . . . . . . . . . . . . . . . . . . . . . . . . .242 19091J-216 . . . . . . . . . . . . . . . . . . . . . . . . . . .242 19091J-231 . . . . . . . . . . . . . . . . . . . . . . . . . . .242 19091J-233 . . . . . . . . . . . . . . . . . . . . . . . . . . .242
Indices
727
Part Number Index

19091J-236 . . . . . . . . . . . . . . . . . . . . . . . . . . .242 19091J-236E . . . . . . . . . . . . . . . . . . . . . . . . . .304 19091J-313 . . . . . . . . . . . . . . . . . . . . . . . . . . .242 19091J-333 . . . . . . . . . . . . . . . . . . . . . . . . . . .242 19091J-411 . . . . . . . . . . . . . . . . . . . . . . . . . . .242 19091J-411E . . . . . . . . . . . . . . . . . . . . . . . . . .304 19091J-413 . . . . . . . . . . . . . . . . . . . . . . . . . . .242 19091J-413E . . . . . . . . . . . . . . . . . . . . . . . . . .304 19091J-416 . . . . . . . . . . . . . . . . . . . . . . . . . . .242 19091J-431 . . . . . . . . . . . . . . . . . . . . . . . . . . .242 19091J-433 . . . . . . . . . . . . . . . . . . . . . . . . . . .242 19091J-433E . . . . . . . . . . . . . . . . . . . . . . . . . .304 19091J-436 . . . . . . . . . . . . . . . . . . . . . . . . . . .242 19091J-436E . . . . . . . . . . . . . . . . . . . . . . . . . .304 19091L-001 . . . . . . . . . . . . . . . . . . . . . . . . . . .252 19091L-002 . . . . . . . . . . . . . . . . . . . . . . . . . . .252 19091L-101 . . . . . . . . . . . . . . . . . . . . . . . . . . .252 19091L-102 . . . . . . . . . . . . . . . . . . . . . . . . . . .252 19091L-105 . . . . . . . . . . . . . . . . . . . . . . . . . . .252 19091L-111 . . . . . . . . . . . . . . . . . . . . . . . . . . .253 19091L-113 . . . . . . . . . . . . . . . . . . . . . . . . . . .253 19091L-113E . . . . . . . . . . . . . . . . . . . . . . . . . .304 19091L-116 . . . . . . . . . . . . . . . . . . . . . . . . . . .253 19091L-131 . . . . . . . . . . . . . . . . . . . . . . . . . . .252 19091L-133 . . . . . . . . . . . . . . . . . . . . . . . . . . .253 19091L-313 . . . . . . . . . . . . . . . . . . . . . . . . . . .253 19091L-331 . . . . . . . . . . . . . . . . . . . . . . . . . . .252 19091L-333 . . . . . . . . . . . . . . . . . . . . . . . . . . .252 19091L-411 . . . . . . . . . . . . . . . . . . . . . . . . . . .253 19091L-413 . . . . . . . . . . . . . . . . . . . . . . . . . . .253 19091L-413E . . . . . . . . . . . . . . . . . . . . . . . . . .304 19091L-416 . . . . . . . . . . . . . . . . . . . . . . . . . . .253 19091L-431 . . . . . . . . . . . . . . . . . . . . . . . . . . .252 19091L-433 . . . . . . . . . . . . . . . . . . . . . . . . . . .252 19091L-436 . . . . . . . . . . . . . . . . . . . . . . . . . . .253 19091N-013 . . . . . . . . . . . . . . . . . . . . . . . . . .264 19091N-013E . . . . . . . . . . . . . . . . . . . . . . . . .305 19091N-016 . . . . . . . . . . . . . . . . . . . . . . . . . .264 19091N-033 . . . . . . . . . . . . . . . . . . . . . . . . . .264 19091N-036 . . . . . . . . . . . . . . . . . . . . . . . . . .264 19091N-102 . . . . . . . . . . . . . . . . . . . . . . . . . .264 19091N-105 . . . . . . . . . . . . . . . . . . . . . . . . . .264 19091N-111 . . . . . . . . . . . . . . . . . . . . . . . . . .264 19091N-113 . . . . . . . . . . . . . . . . . . . . . . . . . .264 19091N-113E . . . . . . . . . . . . . . . . . . . . . . . . .305 19091N-116 . . . . . . . . . . . . . . . . . . . . . . . . . .265 19091N-116E . . . . . . . . . . . . . . . . . . . . . . . . .305 19091N-131 . . . . . . . . . . . . . . . . . . . . . . . . . .264 19091N-133 . . . . . . . . . . . . . . . . . . . . . . . . . .264 19091N-133E . . . . . . . . . . . . . . . . . . . . . . . . .305 19091N-136 . . . . . . . . . . . . . . . . . . . . . . . . . .264 19091N-136E . . . . . . . . . . . . . . . . . . . . . . . . .305 19091N-202 . . . . . . . . . . . . . . . . . . . . . . . . . .264 19091N-205 . . . . . . . . . . . . . . . . . . . . . . . . . .264 19091N-211 . . . . . . . . . . . . . . . . . . . . . . . . . .264 19091N-213 . . . . . . . . . . . . . . . . . . . . . . . . . .264 19091N-213E . . . . . . . . . . . . . . . . . . . . . . . . .305 19091N-216 . . . . . . . . . . . . . . . . . . . . . . . . . .265 19091N-216E . . . . . . . . . . . . . . . . . . . . . . . . .305 19091N-231 . . . . . . . . . . . . . . . . . . . . . . . . . .264 19091N-233 . . . . . . . . . . . . . . . . . . . . . . . . . .264 19091N-233E . . . . . . . . . . . . . . . . . . . . . . . . .305 19091N-236 . . . . . . . . . . . . . . . . . . . . . . . . . .264 19091P-K15 . . . . . . . . . . . . . . . . . . . . . . . . . . .287 19091P-K15E . . . . . . . . . . . . . . . . . . . . . . . . .305 19091P-K33 . . . . . . . . . . . . . . . . . . . . . . . . . . .287 19091P-M15 . . . . . . . . . . . . . . . . . . . . . . . . . .289 19091P-MS3 . . . . . . . . . . . . . . . . . . . . . . . . . .290 19091P-MS4 . . . . . . . . . . . . . . . . . . . . . . . . . .290 19091P-MS4E . . . . . . . . . . . . . . . . . . . . . . . . .305 19091P-MS7 . . . . . . . . . . . . . . . . . . . . . . . . . .290 19091P-MS8 . . . . . . . . . . . . . . . . . . . . . . . . . .290 19091P-QO3 . . . . . . . . . . . . . . . . . . . . . . . . . .291 19091P-QO4 . . . . . . . . . . . . . . . . . . . . . . . . . .291 19091P-QO4E . . . . . . . . . . . . . . . . . . . . . . . . .306 19091P-S15 . . . . . . . . . . . . . . . . . . . . . . . . . . .288 19091P-S15E . . . . . . . . . . . . . . . . . . . . . . . . .305 19091P-S31 . . . . . . . . . . . . . . . . . . . . . . . . . . .288 19091P-S33 . . . . . . . . . . . . . . . . . . . . . . . . . . .288 19091P-UO3 . . . . . . . . . . . . . . . . . . . . . . . . . .292 19091P-UO4 . . . . . . . . . . . . . . . . . . . . . . . . . .292 19091P-UO4E . . . . . . . . . . . . . . . . . . . . . . . . .306 19091R-219 . . . . . . . . . . . . . . . . . . . . . . . . . . .284 19091R-303 . . . . . . . . . . . . . . . . . . . . . . . . . . .284 19091R-306 . . . . . . . . . . . . . . . . . . . . . . . . . . .284 19091R-316 . . . . . . . . . . . . . . . . . . . . . . . . . . .284 19091R-319 . . . . . . . . . . . . . . . . . . . . . . . . . . .284 19091S-001 . . . . . . . . . . . . . . . . . . . . . . . . . . .279 19091S-001E . . . . . . . . . . . . . . . . . . . . . . . . . .306 19091S-010 . . . . . . . . . . . . . . . . . . . . . . . . . . .275 19091S-101 . . . . . . . . . . . . . . . . . . . . . . .225, 245 19091S-102 . . . . . . . . . . . . . . . . . . . . . . .225, 245 19091S-102E . . . . . . . . . . . . . . . . . . . . . . . . . .304 19091S-105 . . . . . . . . . . . . . . . . . . . . . . .225, 245 19091S-112 . . . . . . . . . . . . . . . . . . . . . . .226, 245 19091S-113 . . . . . . . . . . . . . . . . . . . . . . .226, 245 19091S-133 . . . . . . . . . . . . . . . . . . . . . . .225, 245 19091S-211 . . . . . . . . . . . . . . . . . . . . . . .226, 245 19091S-213 . . . . . . . . . . . . . . . . . . . . . . .226, 245 19091S-231 . . . . . . . . . . . . . . . . . . . . . . .225, 245 19091S-233 . . . . . . . . . . . . . . . . . . . . . . .225, 245 19091S-233E . . . . . . . . . . . . . . . . . . . . . . . . . .304 19091S-311 . . . . . . . . . . . . . . . . . . . . . . .226, 245 19091S-313 . . . . . . . . . . . . . . . . . . . . . . .226, 245 19091S-316 . . . . . . . . . . . . . . . . . . . . . . .226, 245 19091S-331 . . . . . . . . . . . . . . . . . . . . . . .225, 245 19091S-333 . . . . . . . . . . . . . . . . . . . . . . .225, 245 19091S-333E . . . . . . . . . . . . . . . . . . . . . . . . . .304 19091S-336 . . . . . . . . . . . . . . . . . . . . . . .225, 245 19091S-411 . . . . . . . . . . . . . . . . . . . . . . .226, 245 19091S-413 . . . . . . . . . . . . . . . . . . . . . . .226, 245 19091S-416 . . . . . . . . . . . . . . . . . . . . . . .226, 245 19091S-431 . . . . . . . . . . . . . . . . . . . . . . .225, 245 19091S-433 . . . . . . . . . . . . . . . . . . . . . . .225, 245 19091S-433E . . . . . . . . . . . . . . . . . . . . . . . . . .304 19091S-436 . . . . . . . . . . . . . . . . . . . . . . .225, 245 19091S-510 . . . . . . . . . . . . . . . . . . . . . . . . . . .273 19091S-510E . . . . . . . . . . . . . . . . . . . . . . . . . .304 19091S-602 . . . . . . . . . . . . . . . . . . . . . . .222, 238 19091S-612 . . . . . . . . . . . . . . . . . . . . . . .222, 238 19091S-633 . . . . . . . . . . . . . . . . . . . . . . .222, 238 19091S-713 . . . . . . . . . . . . . . . . . . . . . . .222, 238 19091S-733 . . . . . . . . . . . . . . . . . . . . . . .222, 238 19091S-813 . . . . . . . . . . . . . . . . . . . . . . .222, 238 19091S-833 . . . . . . . . . . . . . . . . . . . . . . .222, 238 19091S-833E . . . . . . . . . . . . . . . . . . . . . . . . . .303 19091S-911 . . . . . . . . . . . . . . . . . . . . . . .222, 238 19091S-913 . . . . . . . . . . . . . . . . . . . . . . .222, 238 19091S-916 . . . . . . . . . . . . . . . . . . . . . . .222, 238 19091S-931 . . . . . . . . . . . . . . . . . . . . . . .222, 238 19091S-933 . . . . . . . . . . . . . . . . . . . . . . .222, 238 19091S-933E . . . . . . . . . . . . . . . . . . . . . . . . . .303 19091S-936 . . . . . . . . . . . . . . . . . . . . . . .222, 238 19091S-936E . . . . . . . . . . . . . . . . . . . . . . . . . .303 19091W-012 . . . . . . . . . . . . . . . . . . . . . . . . . .295 19091W-015 . . . . . . . . . . . . . . . . . . . . . . . . . .295 19091W-102 . . . . . . . . . . . . . . . . . . . . . . . . . .295 19091W-105 . . . . . . . . . . . . . . . . . . . . . . . . . .295 19091Y-012 . . . . . . . . . . . . . . . . . . . . . . . . . . .294 19091Y-015 . . . . . . . . . . . . . . . . . . . . . . . . . . .294 19091Y-102 . . . . . . . . . . . . . . . . . . . . . . . . . . .294 19091Y-102E . . . . . . . . . . . . . . . . . . . . . . . . . .304 19091Y-105 . . . . . . . . . . . . . . . . . . . . . . . . . . .294 19091Z-002 . . . . . . . . . . . . . . . . . . . . . . . . . . .235 19091Z-005 . . . . . . . . . . . . . . . . . . . . . . . . . . .235 19091Z-012 . . . . . . . . . . . . . . . . . . . . . . .197, 236 19091Z-012E . . . . . . . . . . . . . . . . . . . . . . . . . .303 19091Z-015 . . . . . . . . . . . . . . . . . . . . . . . . . . .236 19091Z-102 . . . . . . . . . . . . . . . . . . . . . . . . . . .235 19091Z-102E . . . . . . . . . . . . . . . . . . . . . . . . . .303 19091Z-105 . . . . . . . . . . . . . . . . . . . . . . . . . . .236 19091Z-112 . . . . . . . . . . . . . . . . . . . . . . . . . . .236 19091Z-112E . . . . . . . . . . . . . . . . . . . . . . . . . .303 19091Z-115 . . . . . . . . . . . . . . . . . . . . . . . . . . .236 19091Z-115E . . . . . . . . . . . . . . . . . . . . . . . . . .303 19091Z-202 . . . . . . . . . . . . . . . . . . . . . . . . . . .235 19091Z-205 . . . . . . . . . . . . . . . . . . . . . . .236, 279 19091Z-211 . . . . . . . . . . . . . . . . . . . . . . . . . . .236 19091Z-211E . . . . . . . . . . . . . . . . . . . . . . . . . .303 19091Z-212 . . . . . . . . . . . . . . . . . . . . . . . . . . .236 19091Z-213 . . . . . . . . . . . . . . . . . . . . . . . . . . .236 19091Z-213E . . . . . . . . . . . . . . . . . . . . . . . . . .303 19091Z-215 . . . . . . . . . . . . . . . . . . . . . . . . . . .236 19091Z-216 . . . . . . . . . . . . . . . . . . . . . . . . . . .236 19091Z-216E . . . . . . . . . . . . . . . . . . . . . . . . . .303 19091Z-231 . . . . . . . . . . . . . . . . . . . . . . . . . . .236 19091Z-233 . . . . . . . . . . . . . . . . . . . . . . . . . . .236 19091Z-233E . . . . . . . . . . . . . . . . . . . . . . . . . .303 19091Z-236 . . . . . . . . . . . . . . . . . . . . . . . . . . .236 19091Z-236E . . . . . . . . . . . . . . . . . . . . . . . . . .303 19091Z-313 . . . . . . . . . . . . . . . . . . . . . . . . . . .236 19091Z-331 . . . . . . . . . . . . . . . . . . . . . . . . . . .236 19091Z-333 . . . . . . . . . . . . . . . . . . . . . . . . . . .236 19091Z-411 . . . . . . . . . . . . . . . . . . . . . . . . . . .236
Indices
728
Part Number Index

19091Z-411E . . . . . . . . . . . . . . . . . . . . . . . . . .303 19091Z-413 . . . . . . . . . . . . . . . . . . . . . . . . . . .236 19091Z-413E . . . . . . . . . . . . . . . . . . . . . . . . . .303 19091Z-416 . . . . . . . . . . . . . . . . . . . . . . . . . . .236 19091Z-431 . . . . . . . . . . . . . . . . . . . . . . . . . . .236 19091Z-433 . . . . . . . . . . . . . . . . . . . . . . . . . . .236 19091Z-433E . . . . . . . . . . . . . . . . . . . . . . . . . .303 19091Z-436 . . . . . . . . . . . . . . . . . . . . . . . . . . .236 19091Z-436E . . . . . . . . . . . . . . . . . . . . . . . . . .303 19091Z-513 . . . . . . . . . . . . . . . . . . . . . . . . . . .236 19091Z-513E . . . . . . . . . . . . . . . . . . . . . . . . . .303 19091Z-530 . . . . . . . . . . . . . . . . . . . . . . .236, 279 19091Z-530E . . . . . . . . . . . . . . . . . . . . . . . . . .303 19091Z-613 . . . . . . . . . . . . . . . . . . . . . . . . . . .236 19091Z-713 . . . . . . . . . . . . . . . . . . . . . . . . . . .236 19091Z-713E . . . . . . . . . . . . . . . . . . . . . . . . . .303 19091Z-716 . . . . . . . . . . . . . . . . . . . . . . . . . . .236 19095F-120 . . . . . . . . . . . . . . . . . . . . . . . . . . .266 19095F-120E . . . . . . . . . . . . . . . . . . . . . . . . . .305 19095F-121 . . . . . . . . . . . . . . . . . . . . . . . . . . .266 19095F-123 . . . . . . . . . . . . . . . . . . . . . . . . . . .266 19095F-123E . . . . . . . . . . . . . . . . . . . . . . . . . .305 19095J-023 . . . . . . . . . . . . . . . . . . . . . . . . . . .242 19095J-023E . . . . . . . . . . . . . . . . . . . . . . . . . .304 19095J-121 . . . . . . . . . . . . . . . . . . . . . . . . . . .242 19095J-121E . . . . . . . . . . . . . . . . . . . . . . . . . .304 19095J-123 . . . . . . . . . . . . . . . . . . . . . . . . . . .242 19095J-123E . . . . . . . . . . . . . . . . . . . . . . . . . .304 19095J-321 . . . . . . . . . . . . . . . . . . . . . . . . . . .242 19095J-323 . . . . . . . . . . . . . . . . . . . . . . . . . . .242 19095J-323E . . . . . . . . . . . . . . . . . . . . . . . . . .304 19095J-621 . . . . . . . . . . . . . . . . . . . . . . . . . . .242 19095J-623 . . . . . . . . . . . . . . . . . . . . . . . . . . .242 19095L-021 . . . . . . . . . . . . . . . . . . . . . . . . . . .253 19095L-023 . . . . . . . . . . . . . . . . . . . . . . . . . . .253 19095L-023E . . . . . . . . . . . . . . . . . . . . . . . . . .304 19095L-121 . . . . . . . . . . . . . . . . . . . . . . . . . . .294 19095L-523 . . . . . . . . . . . . . . . . . . . . . . . . . . .253 19095N-121 . . . . . . . . . . . . . . . . . . . . . . . . . .265 19095N-121E . . . . . . . . . . . . . . . . . . . . . . . . .305 19095N-123 . . . . . . . . . . . . . . . . . . . . . . . . . .265 19095N-123E . . . . . . . . . . . . . . . . . . . . . . . . .305 19095N-126 . . . . . . . . . . . . . . . . . . . . . . . . . .265 19095P-K23 . . . . . . . . . . . . . . . . . . . . . . . . . . .287 19095P-K25 . . . . . . . . . . . . . . . . . . . . . . . . . . .287 19095P-M23 . . . . . . . . . . . . . . . . . . . . . . . . . .289 19095P-M25 . . . . . . . . . . . . . . . . . . . . . . . . . .289 19095P-M25E . . . . . . . . . . . . . . . . . . . . . . . . .305 19095P-MS0 . . . . . . . . . . . . . . . . . . . . . . . . . .290 19095P-MS0E . . . . . . . . . . . . . . . . . . . . . . . . .305 19095P-MS5 . . . . . . . . . . . . . . . . . . . . . . . . . .290 19095P-MS6 . . . . . . . . . . . . . . . . . . . . . . . . . .290 19095P-MS6E . . . . . . . . . . . . . . . . . . . . . . . . .305 19095P-MS9 . . . . . . . . . . . . . . . . . . . . . . . . . .290 19095P-QO3 . . . . . . . . . . . . . . . . . . . . . . . . . .291 19095P-QO3E . . . . . . . . . . . . . . . . . . . . . . . . .306 19095P-QO4 . . . . . . . . . . . . . . . . . . . . . . . . . .291 19095P-QO4E . . . . . . . . . . . . . . . . . . . . . . . . .306 19095P-S21 . . . . . . . . . . . . . . . . . . . . . . . . . . .288 19095P-S23 . . . . . . . . . . . . . . . . . . . . . . . . . . .288 19095P-S25 . . . . . . . . . . . . . . . . . . . . . . . . . . .288 19095P-S25E . . . . . . . . . . . . . . . . . . . . . . . . .305 19095P-UO3 . . . . . . . . . . . . . . . . . . . . . . . . . .292 19095P-UO4 . . . . . . . . . . . . . . . . . . . . . . . . . .292 19095P-UO4E . . . . . . . . . . . . . . . . . . . . . . . . .306 19095R-420 . . . . . . . . . . . . . . . . . . . . . . . . . . .284 19095R-429 . . . . . . . . . . . . . . . . . . . . . . . . . . .284 19095S-100 . . . . . . . . . . . . . . . . . . . . . . . . . . .236 19095S-100E . . . . . . . . . . . . . . . . . . . . . . . . . .303 19095V-420 . . . . . . . . . . . . . . . . . . . . . . . . . . .273 19095V-420E . . . . . . . . . . . . . . . . . . . . . . . . . .304 19095W-121 . . . . . . . . . . . . . . . . . . . . . . . . . .295 19095W-123 . . . . . . . . . . . . . . . . . . . . . . . . . .295 19095Z-020 . . . . . . . . . . . . . . . . . . . . . . . . . . .236 19095Z-021 . . . . . . . . . . . . . . . . . . . . . . . . . . .236 19095Z-023 . . . . . . . . . . . . . . . . . . . . . . . . . . .237 19095Z-121 . . . . . . . . . . . . . . . . . . . . . . . . . . .236 19095Z-121E . . . . . . . . . . . . . . . . . . . . . . . . . .303 19095Z-123 . . . . . . . . . . . . . . . . . . . . . . . . . . .237 19095Z-123E . . . . . . . . . . . . . . . . . . . . . . . . . .303 19095Z-220 . . . . . . . . . . . . . . . . . . . . . . . . . . .236 19095Z-220E . . . . . . . . . . . . . . . . . . . . . . . . . .303 19095Z-221 . . . . . . . . . . . . . . . . . . . . . . . . . . .236 19095Z-221E . . . . . . . . . . . . . . . . . . . . . . . . . .303 19095Z-321 . . . . . . . . . . . . . . . . . . . . . . . . . . .236 19095Z-323 . . . . . . . . . . . . . . . . . . . . . . . . . . .237 19095Z-323E . . . . . . . . . . . . . . . . . . . . . . . . . .303 19095Z-421 . . . . . . . . . . . . . . . . . . . . . . . . . . .236 19095Z-421E . . . . . . . . . . . . . . . . . . . . . . . . . .303 19095Z-423 . . . . . . . . . . . . . . . . . . . . . . . . . . .237 19095Z-423E . . . . . . . . . . . . . . . . . . . . . . . . . .303 19095Z-621 . . . . . . . . . . . . . . . . . . . . . . . . . . .236 19095Z-621E . . . . . . . . . . . . . . . . . . . . . . . . . .303 19095Z-623 . . . . . . . . . . . . . . . . . . . . . . . . . . .237 19095Z-623E . . . . . . . . . . . . . . . . . . . . . . . . . .303 19095Z-626 . . . . . . . . . . . . . . . . . . . . . . . . . . .237 19095Z-627 . . . . . . . . . . . . . . . . . . . . . . . . . . .236 191-1311 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .660 191-3211 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .660 191-5211 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .660 19199M . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .71 19199N . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .71 19199P . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .72 192-3211 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .660 192-5211 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .660 19231-20910 . . . . . . . . . . . . . . . . . . . . . .182, 192 19231-20940 . . . . . . . . . . . . . . . . . . . . . .182, 192 19231-20950 . . . . . . . . . . . . . . . . . . . . . . . . . .192 19231-20960 . . . . . . . . . . . . . . . . . . . . . . . . . .192 19231-20970 . . . . . . . . . . . . . . . . . . . . . . . . . .192 19231-21050 . . . . . . . . . . . . . . . . . . . . . .182, 192 19231-21060 . . . . . . . . . . . . . . . . . . . . . .182, 192 19231-21080 . . . . . . . . . . . . . . . . . . . . . . . . . .192 19231-60680 . . . . . . . . . . . . . . . . . . . . . .182, 192 19231-60690 . . . . . . . . . . . . . . . . . . . . . . . . . .192 19231-60790 . . . . . . . . . . . . . . . . . . . . . . . . . . .70 19231-80520 . . . . . . . . . . . . . . . . . . . . . .185, 193 19231-80530 . . . . . . . . . . . . . . . . . . . . . .185, 193 19231-80590 . . . . . . . . . . . . . . . . . . . . . . . . . . .70 19232-60676 . . . . . . . . . . . . . . . . . . . . . . . . . .194 19232-80550 . . . . . . . . . . . . . . . . . . . . . . . . . .194 19233-20625 . . . . . . . . . . . . . . . . . . . . . .187, 194 19233-20755 . . . . . . . . . . . . . . . . . . . . . .187, 194 19233-80565 . . . . . . . . . . . . . . . . . . . . . . . . . .194 19234-60540 . . . . . . . . . . . . . . . . . . . . . . . . . .193 19234-60720 . . . . . . . . . . . . . .180, 185, 187, 192 19243-00067 . . . . . . . . . . . . . . . . . .176, 180, 188 19243-00070 . . . . . . . . . . . . . . . . . . . . . .176, 188 19243-60500 . . . . . . . . . . . . . . . . . . . . . . . . . .192 19243-60505 . . . . . . . . . . . . . . . . . . . . . . . . . .191 19243-60570 . . . . . . . . . . . . . . . . . . . . . . . . . .191 19243-80510 . . . . . . . . . . . . . . . . . . . . . . . . . .191 19243-80520 . . . . . . . . . . . . . . . . . . . . . . . . . .191 19243-80530 . . . . . . . . . . . . . . . . . . . . . .180, 191 19243-80540 . . . . . . . . . . . . . . . . . . . . . .180, 191 19243-80570 . . . . . . . . . . . . . . . . . . . . . .180, 191 19244-80540 . . . . . . . . . . . . . . . . . . . . . .180, 191 19244-80560 . . . . . . . . . . . . .182184, 192193 19244-80610 . . . . . . . . . . . . . . . . . . . . . .185, 193 19244-80620 . . . . . . . . . . . . . . . . . . . . . .183, 193 19245-20510 . . . . . . . . . . . . . . . . . .178179, 190 19245-20515 . . . . . . . . . . . . . . . . . .178179, 190 19245-20525 . . . . . . . . . . . . . . . . . .178179, 190 19245-20550 . . . . . . . . . . . . . . . . . . . . . . . . . .190 19245-20580 . . . . . . . . . . . . . . . . . .178179, 190 19245-20670 . . . . . . . . . . . . . . . . . . . . . . . . . .190 19245-20780 . . . . . . . . . . . . . . . . . . . . . .178, 190 19245-40050 . . . . . . . . . . . . . . . . . . . . . .178, 190 19245-60760 . . . . . . . . . . . . . . . . . . . . . .178, 190 19245-80520 . . . . . . . . . . . . . . . . . . . . . . . . . .190 19245-80521 . . . . . . . . . . . . . . . . . . . . . .178179 19246-60526 . . . . . . . . . . . . . . . . . . . . . . . . . .198 19246-60560 . . . . . . . . . . . . . . . . . . . . . . . . . .198 19246-60570 . . . . . . . . . . . . . . . . . . . . . . . . . .198 19246-60580 . . . . . . . . . . . . . . . . . . . . . . . . . .198 19246-60620 . . . . . . . . . . . . . . . . . . . . . . . . . .198 19246-60630 . . . . . . . . . . . . . . . . . . . . . . . . . .198 19246-60690 . . . . . . . . . . . . . . . . . . . . . . . . . .198 19251-00100 . . . . . . . . . . . . . . . . . . . . . .176, 188 19251-20620 . . . . . . . . . . . . . . . . . . . . . . . . . .188 19251-60540 . . . . . . . . . . . . . .157, 176, 188189 19251-60575 . . . . . . . . . . . . . . . . . . . . . .175, 188 19251-80525 . . . . . . . . . . . . . . . . . . . . . . . . . .188 19251-80680 . . . . . . . . . . . . . . . . . . .89, 189, 314 19251C . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .70 19256-00090 . . . . . . . . . . . . . . . . . . . . . . . . . .187 19256-00200 . . . . . . . . . . . . . . . . . . . . . . . . . .187 19256-20510 . . . . . . . . . . . . . . . . . . . . . . . . . .187 19256-20550 . . . . . . . . . . . . . . . . . . . . . . . . . .187 19256-20580 . . . . . . . . . . . . . . . . . . . . . . . . . .187 19256-20590 . . . . . . . . . . . . . . . . . . . . . . . . . .187 19256-60500 . . . . . . . . . . . . . . . . . . . . . . . . . .195 19256-60590 . . . . . . . . . . . . . . . . . . . . . . . . . .195 19256-60700 . . . . . . . . . . . . . . . . . . . . . .187, 195
Indices
729
Part Number Index

19256-80000 . . . . . . . . . . . . . . . . . . . . . . . . . .195 19256-80010 . . . . . . . . . . . . . . . . . . . . . . . . . .195 19256-80030 . . . . . . . . . . . . . . . . . . . . . .187, 195 19256-80040 . . . . . . . . . . . . . . . . . . . . . . . . . .187 19256-80060 . . . . . . . . . . . . . . . . . . . . . .187, 195 19256-80560 . . . . . . . . . . . . . . . . . . . . . . . . . .187 19256-80570 . . . . . . . . . . . . . . . . . . . . . . . . . .195 19256-80590 . . . . . . . . . . . . . . . . . . . . . . . . . .195 19258-20830 . . . . . . . . . . . . . . . . . . . . . . . . . .174 19258-20870 . . . . . . . . . . . . . . . . . . . . . . . . . .174 19258-20920 . . . . . . . . . . . . . . . . . . . . . . . . . .164 19298-60500 . . . . . . . . . . . . . . . . . . . . . . . . . .100 19301-00150 . . . . . . . . . . . . . . . . . . . . . . . . . .182 19301-20720 . . . . . . . . . . . . . . . . . .182183, 192 19301-60660 . . . . . . . . . . . . .182183, 192193 19301-80530 . . . . . . . . . . . . . . . . . . . . . . . . . .194 19302-80020 . . . . . . . . . . . . . . . . . . . . . . . . . .194 19305-60580 . . . . . . . . . . . . . . . . . . . . . . .98, 195 19320-60656 . . . . . . . . . . . . . . . . . . . . . . . . . .198 19320-80625 . . . . . . . . . . . . . . . . . . . . . .178, 190 19325-60660 . . . . . . . . . . . . . . . . . . . . . . . . . .168 19350-60540 . . . . . . . . . . . . . . . . . . . . . . . . . .102 19354-60510 . . . . . . . . . . . . . . . . . . . . . . . . . .100 19361-60566 . . . . . . . . . . . . . . . . . . . . . . . . . .198 19362-60510 . . . . . . . . . . . . . . . . . . . . . . . . . .198 19362-60515 . . . . . . . . . . . . . . . . . . . . . . . . . .198 19362-60520 . . . . . . . . . . . . . . . . . . . . . . . . . .198 19362-60525 . . . . . . . . . . . . . . . . . . . . . . . . . .198 19362-60530 . . . . . . . . . . . . . . . . . . . . . . . . . .198 19362-60535 . . . . . . . . . . . . . . . . . . . . . . . . . .198 19362-60540 . . . . . . . . . . . . . . . . . . . . . . . . . .198 19362-60545 . . . . . . . . . . . . . . . . . . . . . . . . . .198 19362-60550 . . . . . . . . . . . . . . . . . . . . . . . . . .198 19362-60555 . . . . . . . . . . . . . . . . . . . . . . . . . .198 19362-60565 . . . . . . . . . . . . . . . . . . . . . . . . . .198 19362-60575 . . . . . . . . . . . . . . . . . . . . . . . . . .198 19363-60506 . . . . . . . . . . . . . . . . . . . . . . . . . .198 194-8111 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .665 196-7203 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .665 197-7202 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .665 199-2602 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .665 200-0001 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .96 200-0010 . . . . . . . . . . . . . . . . . . . . . . . . . . .9697 200-0012 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .97 200-0014 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .96 200-0015 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .97 200-0016 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .97 200-0017 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .96 200-0019 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .96 200-0020 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .96 200-0021 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .96 200-0024 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .97 200-0025 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .96 200-0032 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .96 200-0056 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .96 200-0061 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .97 200-0064 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .97 200-0066 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .96 200-0070 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .97 200-0091 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .96 200-0092 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .97 200-0102 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .96 200-0103 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .96 200-0107 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .96 200-0110 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .96 200-0111 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .97 200-0113 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .96 200-0117 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .96 200-0123 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .96 200-0132 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .97 200-0185 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .96 200-0200 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .96 200-0210 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .97 200-0225 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .96 200-0317 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .96 210-1063 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .159 210-1063-G . . . . . . . . . . . . . . . . . . . . . . . . . . .159 210-1064 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .159 210-1064-G . . . . . . . . . . . . . . . . . . . . . . . . . . .159 210-1066 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .159 210-1066-G . . . . . . . . . . . . . . . . . . . . . . . . . . .159 210-1070 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .159 210-2063H22 . . . . . . . . . . . . . . . . . . . . . . . . .158 210-2063H26 . . . . . . . . . . . . . . . . . . . . . . . . .158 210-3003 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .157 210-3003-5 . . . . . . . . . . . . . . . . . . . . . . . . . . .157 210-4004-5 . . . . . . . . . . . . . . . . . . . . . . . . . . .158 210-4022-5 . . . . . . . . . . . . . . . . . . . . . . . . . . .158 2140-0549 . . . . . . . . . . . . . . . . . . . . . . . . . . . .499 2140-0585 . . . . . . . . . . . . . . . . . . . . . . . . . . . .656 2140-0590 . . . . . . . . . . . . . . . . . . . . . . . .455, 478 2140-0600 . . . . . . . . . . . . . . . . . . . . . . . .455, 481 2140-0605 . . . . . . . . . . . . . . . . . . . . . . . . . . . .697 2190-0108 . . . . . . . . . . . . . . . . . . . . . . . . . . . .187 220-1170 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .77 220-1171-E . . . . . . . . . . . . . . . . . . . . . . . . . . . .77 220-1171-U . . . . . . . . . . . . . . . . . . . . . . . . . . . .77 280959-001 . . . . . . . . . . . . . . . . . . . . . . . . . . .518 280959-006 . . . . . . . . . . . . . . . . . . . . . . . . . . .518 280959-904 . . . . . . . . . . . . . . . . . . . . . . .448, 518 280959-905 . . . . . . . . . . . . . . . . . . . . . . . . . . .518 300-301-HSP . . . . . . . . . . . . . . . . . . . . . . . . . .174 301-011-HSP . . . . . . . . . . . . . . . . . . . . . . . . . .174 301-013-HSP . . . . . . . . . . . . . . . . . . . . . . . . . .174 301-015-HSP . . . . . . . . . . . . . . . . . . . . . . . . . .174 301-016-HSP . . . . . . . . . . . . . . . . . . . . . . . . . .174 301-017-HSP . . . . . . . . . . . . . . . . . . . . . . . . . .174 301-152-HSP . . . . . . . . . . . . . . . . . . . . . . . . . .174 301-169-HSP . . . . . . . . . . . . . . . . . . . . . . . . . .174 301-170-HSP . . . . . . . . . . . . . . . . . . . . . . . . . .175 301-205-HSP . . . . . . . . . . . . . . . . . . . . . . . . . .175 301-211-HSP . . . . . . . . . . . . . . . . . . . . . . . . . .174 301-212-HSP . . . . . . . . . . . . . . . . . . . . . . . . . .174 301-220-HSP . . . . . . . . . . . . . . . . . . . . . . . . . .174 301-221-HSP . . . . . . . . . . . . . . . . . . . . . . . . . .174 301-222-HSP . . . . . . . . . . . . . . . . . . . . . . . . . .174 3050-0891 . . . . . . . . . . . . . . . . . . . . . . . . . . . .206 3150-0374 . . . . . . . . . . . . . . . . . . . . . . . . . . . .197 3150-0417 . . . . . . . . . . . . . . . . . . . . . . . . . . . .197 3150-0462 . . . . . . . . . . . . . . . . . . . . . . . . . . . .492 3150-0463 . . . . . . . . . . . . . . . . . . . . . . . . . . . .492 3150-0507 . . . . . . . . . . . . . . . . . . . . . . . . . . . .449 3150-0508 . . . . . . . . . . . . . . . . . . . . . . . . . . . .449 3150-0509 . . . . . . . . . . . . . . . . . . . . . . . . . . . .449 3150-0559 . . . . . . . . . . . . . . . . . . . . . . . . . . . .484 3150-0576 . . . . . . . . . . . . . . . . . . . . . . . . . . . .449 3150-0577 . . . . . . . . . . . . . . . . . . . . . . . . . . . .449 3150-0602 . . . . . . . . . . . . . . . . . . . . . . . . . . . .177 3150-0617 . . . . . . . . . . . . . . . . . . . . . . . . . . . .197 3150-0619 . . . . . . . . . . . . . . . . . . . . . . . . . . . .656 3150-0753 . . . . . . . . . . . . . . . . . . . . . . . . . . . .146 3150-0754 . . . . . . . . . . . . . . . . . . . . . . . . . . . .146 3150-0942 . . . . . . . . . . . . . . . . . . . . . . . . . . . .492 3150-0944 . . . . . . . . . . . . . . . . . . . . . . . . . . . .465 3162-0110 . . . . . . . . . . . . . . . . . . . . . . . . . . . .215 3162-1056 . . . . . . . . . . . . . . . . . . . . . . . .678, 687 321-002-HSP . . . . . . . . . . . . . . . . . . . . . . . . . .174 321-055-HSP . . . . . . . . . . . . . . . . . . . . . . . . . .174 321-056-HSP . . . . . . . . . . . . . . . . . . . . . . . . . .174 321-057-HSP . . . . . . . . . . . . . . . . . . . . . . . . . .175 321-058-HSP . . . . . . . . . . . . . . . . . . . . . . . . . .174 321-059-HSP . . . . . . . . . . . . . . . . . . . . . . . . . .174 325-024-HSP . . . . . . . . . . . . . . . . . . . . . . . . . .175 325-045-HSP . . . . . . . . . . . . . . . . . . . . . . . . . .174 325-062-HSP . . . . . . . . . . . . . . . . . . . . . . . . . .175 325-132-HSP . . . . . . . . . . . . . . . . . . . . . . . . . .175 325-185-HSP . . . . . . . . . . . . . . . . . . . . . . . . . .175 325-455-HSP . . . . . . . . . . . . . . . . . . . . . . . . . .175 330-055-HSP . . . . . . . . . . . . . . . . . . . . . . . . . .174 330-086-HSP . . . . . . . . . . . . . . . . . . . . . . . . . .174 3395B . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .104 3396C . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .104 35900-60600 . . . . . . . . . . . . . . . . . . . . . .104105 35900-60610 . . . . . . . . . . . . . . . . . . . . . .104105 35900-60630 . . . . . . . . . . . . . . . . . . . . . . . . . .105 35900-60670 . . . . . . . . . . . . . . . . . . . . . . . . . .106 35900-60700 . . . . . . . . . . . . . . . . . . . . . . . . . .106 35900-60750 . . . . . . . . . . . . . . . . . . . . . .104105 3980-0672 . . . . . . . . . . . . . . . . . . . . . . . .455, 494 420-1000 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .88 420-2000 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .88 425-553-HSP . . . . . . . . . . . . . . . . . . . . . . . . . .174 425-554-HSP . . . . . . . . . . . . . . . . . . . . . . . . . .174 430-1020 . . . . . . . . . . . . . . . . . . . . . . . . . .89, 314 430-2000 . . . . . . . . . . . . . . . . . . . . . . . . . .88, 314 430-3000 . . . . . . . . . . . . . . . . . . . . . . . . . .88, 314 440-1000 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .91 450-1000 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .91 460-1002 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .90 480-0003 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .90 500-1060 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .159 500-1200 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .164 500-2066 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .159 500-2114 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .162
Indices
730
Part Number Index

500-2118 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .162 5001-3702 . . . . . . . . . . . . . . . . . . . . . . . . . . . .471 5001-3707 . . . . . . . . . . . . . . . . . . . .462463, 485 5001-3739 . . . . . . . . . . . . . . . . . . . . . . . . . . . .462 5001-3743 . . . . . . . . . . . . . . . . . . . . . . . . . . . .462 5001-3755 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .38 5001-3756 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .38 5001-3758 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .38 5001-3759 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .38 501-0095 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .591 5020-8292 . . . . . . . . . . . . . . . . . . . . . . . . . . . .163 5020-8293 . . . . . . . . . . . . . . . . . . . . . . . . . . . .163 5020-8294 . . . . . . . . . . . . . . . . . . . . . . . .163, 189 5021-1816 . . . . . . . . . . . . . . . . . . . . . . . .446, 459 5021-1817 . . . . . . . . . . . . . . . . . . . . . . . .446, 459 5021-1818 . . . . . . . . . . . . . . . . . . . . . . . .446, 459 5021-1819 . . . . . . . . . . . . . . . . . . . . . . . .446, 459 5021-1820 . . . . . . . . . . . . . . . . . . . . . . . .446, 459 5021-1821 . . . . . . . . . . . . . . . . . . . . . . . .446, 459 5021-1822 . . . . . . . . . . . . . . . . . . . . . . . .446, 459 5021-1823 . . . . . . . . . . . . . . . .446, 459, 476, 490 5021-1845 . . .513, 529, 540, 565566, 568571, 575, 577, 599 5021-1846 . . . . . . . . . . . . . . . . . . . . . . . . . . . .513 5021-1859 . . . . . . . . . . . . . . . . . . . . . . . . . . . .488 5021-1860 . . . . . . . . . . . . . . . . . . . . . . . . . . . .165 5021-1861 . . . . . . . . . . . . . . . . . . . . . . . . . . . .165 5021-1866 . . . . . . . . . . . . . . . . . . . . . . . . . . . .500 5021-1867 . . . . . . . . . . . . . . . . . . . . . . . . . . . .500 5021-1870 . . . . . . . . . . . . . . . . . . . . . . . . . . . .697 5021-1871 . . . . . . . . . . . . . . . . . . . . . . . . . . . .488 5021-7107 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .82 5021-7113 . . . . . . . . . . . . . . . . . . . . . . . .492493 5021-7127 . . . . . . . . . . . . . . . . . . . . . . . .485, 492 5021-7133 . . . . . . . . . . . . . . . . . . . . . . . .166, 196 5021-7134 . . . . . . . . . . . . . . . . . . . . . . . .166, 196 5021-7136 . . . . . . . . . . . . . . . . . . . . . . . .166, 196 5021-7137 . . . . . . . . . . . . . . . . . . . . . . . .166, 196 5021-7146 . . . . . . . . . . . . . . . . . . . . . . . . . . . .165 5021-7148 . . . . . . . . . . . . . . . . . . . . . . . . . . . .165 5021-7164 . . . . . . . . . . . . . . . . . . . . . . . . . . . .165 5021-7166 . . . . . . . . . . . . . . . . . . . . . . . . . . . .165 5021-7168 . . . . . . . . . . . . . . . . . . . . . . . . . . . .166 5021-7169 . . . . . . . . . . . . . . . . . . . . . . . . . . . .166 5021-7170 . . . . . . . . . . . . . . . . . . . . . . . . . . . .166 5022-2141 . . . . . . . . . . . . . . . . . . . . . . . . . . . .657 5022-2145 . . . . . . . . . . . . . . . . . . . . . . . . . . . .480 5022-2146 . . . . . . . . . . . . . . . . . . . . . . . . . . . .480 5022-2154 . . . . . . . . . . . . . . . . . . . . . . . . . . . .695 5022-2155 . . . . . . . . . . . . . . . . . . . . . . . . . . . .695 5022-2156 . . . . . . . . . . . . . . . . . . . . . . . . . . . .695 5022-2157 . . . . . . . . . . . . . . . . . . . . . . . . . . . .695 5022-2158 . . . . . . . . . . . . . . . . . . . . . . . . . . . .695 5022-2165 . . . . . . . . . . . . . . . . . . . . . . . . . . . .483 5022-2166 . . . . . . . . . . . . . . . . . . . . . . . . . . . .483 5022-2175 . . . . . . . . . . . . . . . . . . . . . . . .455, 469 5022-2185 . . . . . . . . . . . . . . . . . . . . . . . . . . . .464 5022-2192 . . . . . . . . . . . . . . . . . . . . . . . . . . . .465 5041-2163 . . . . . . . . . . . . . . . . . . . . . . . . . . . .485 5041-2166 . . . . . . . . . . . . . . . . . . . . . . . . . . . .695 5041-2167 . . . . . . . . . . . . . . . . . . . . . . . . . . . .697 5041-2168 . . . . . . . . . . . . . . . .460, 464, 486, 656 5041-2171 . . . . . . . . . . . . . . . . . . . . . . . . . . . .164 5041-2172 . . . . . . . . . . . . . . . . . . . . . . . . . . . .164 5041-2173 . . . . . . . . . . . . . . . . . . . . . . . . . . . .164 5041-2174 . . . . . . . . . . . . . . . . . . . . . . . . . . . .164 5041-2175 . . . . . . . . . . . . . . . . . . . . . . . . . . . .164 5041-2176 . . . . . . . . . . . . . . . . . . . . . . . . . . . .164 5041-2178 . . . . . . . . . . . . . . . . . . . . . . . . . . . .164 5041-2179 . . . . . . . . . . . . . . . . . . . . . . . . . . . .165 5041-2184 . . . . . . . . . . . . . . . . . . . . . . . . . . . .695 5041-2185 . . . . . . . . . . . . . . . . . . . . . . . . . . . .695 5041-2191 . . . . . . . . . . . . . . . . . . . . . . . . . . . .695 5041-8339 . . . . . . . . . . . . . . . . . . . .485, 492493 5042-0901 . . . . . . . . . . . . . . . . . . . . . . . .678, 687 5042-0922 . . . . . . . . . . . . . . . . . . . . . . . .669, 679 5042-0923 . . . . . . . . . . . . . . . . . . . . . . . .669, 679 5042-1330 . . . . . . . . . . . . . . . . . . . . . . . . . . . .698 5042-1331 . . . . . . . . . . . . . . . . . . . . . . . . . . . .698 5042-1332 . . . . . . . . . . . . . . . . . . . . . . . . . . . .698 5042-1333 . . . . . . . . . . . . . . . . . . . . . . . . . . . .697 5042-1334 . . . . . . . . . . . . . . . . . . . . . . . . . . . .697 5042-1336 . . . . . . . . . . . . . . . . . . . . . . . .695, 697 5042-1337 . . . . . . . . . . . . . . . . . . . . . . . .695, 698 5042-1356 . . . . . . . . . . . . . . . . . . . . . . . . . . . .697 5042-1370 . . . . . . . . . . . . . . . . . . . . . . . . . . . .146 5042-1385 . . . . . . . . . . . . . . . . . . . . . . . .136, 469 5042-1386 . . . . . . . . . . . . . . . . . . . . . . . .136, 469 5042-1387 . . . . . . . . . . . . . . . . . . . . . . . . . . . .136 5042-1388 . . . . . . . . . . . . . . . . . . . . . . . . . . . .469 5042-1389 . . . . . . . . . . . . . . . . . . . . . . . .136, 469 5042-1391 . . . . . . . . . . . . . . . . . . . . . . . . . . . .147 5042-1392 . . . . . . . . . . . . . . . . . . . . . . . . . . . .147 5042-6443 . . . . . . . . . . . . . . . . . . . . . . . . . . . .481 5042-6454 . . . . . . . . . . . . . . . . . . . . . . . . . . . .469 5042-6458 . . . . . . . . . . . . . . . . . . . . . . . . . . . .465 5042-6459 . . . . . . . . . . . . . . . . . . . . . . . . . . . .465 5042-6461 . . . . . . . . . . . . . . . . . . . . . . . . . . . .446 5042-6462 . . . . . . . . . . . . . . . . . . . . . . . . . . . .446 5042-6463 . . . . . . . . . . . . . . . . . . . . . . . . . . . .446 5042-6470 . . . . . . . . . . . . . . . . . . . . . . . . . . . .465 5060-9077 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .55 5060-9084 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .55 5060-9086 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .99 5060-9096 . . . . . . . . . . . . . . . . . . . . . . . . . .58, 71 5061-3303 . . . . . . . . . . . . . . . . . . . . . . . .447, 481 5061-3304 . . . . . . . . . . . . . . . . . . . . . . . . . . . .446 5061-3305 . . . . . . . . . . . . . . . . . . . . . . . . . . . .518 5061-3306 . . . . . . . . . . . . . . . . . . . . . . . . . . . .518 5061-3315 . . . . . . . . . . . . . . . . . . . . . . . . . . . .446 5061-3327 . . . . . . . . . . . . . . . . . . . . . . . .481, 499 5061-3328 . . . . . . . . . . . . . . . . . . . . . . . .481, 499 5061-3329 . . . . . . . . . . . . . . . . . . . . . . . .481, 499 5061-3330 . . . . . . . . . . . . . . . . . . . . . . . . . . . .598 5061-3331 . . . . . . . . . . . . . . . . . . . . . . . . . . . .598 5061-3332 . . . . . . . . . . . . . . . . . . . . . . . . . . . .598 5061-3333 . . . . . . . . . . . . . . . . . . . . . . . . . . . .598 5061-3334 . . . . . . . . . . . . . . . . . . . . . . . . . . . .598 5061-3335 . . . . . . . . . . . . . . . . . . . . . . . . . . . .599 5061-3337 . . . . . . . . . . . . . . . . . . . . . . . . . . . .599 5061-3339 . . . . . . . . . . . . . . . . . . . . . . . . . . . .599 5061-3349 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .38 5061-3354 . . . . . . . . . . . . . . . . . . . . . . . . . . . .147 5061-3361 . . . . . . . . . . . . . . . . . . . . . . . . . . . .445 5061-3362 . . . . . . . . . . . . . . . . . . . . . . . . . . . .445 5061-3363 . . . . . . . . . . . . . . . . . . . . . . . . . . . .146 5061-3364 . . . . . . . . . . . . . . . . . . . . . . . . . . . .147 5061-3365 . . . . . . . . . . . . . . . . . . . . . . . . . . . .147 5061-3366 . . . . . . . . . . . . . . . . . . . . . . . . . . . .147 5061-3367 . . . . . . . . . . . . . . . . . . . . . . . .146, 481 5061-3368 . . . . . . . . . . . . . . . . . . . . . . . . . . . .146 5061-3384 . . . . . . . . . . . . . . . . . . . . . . . . . . . .692 5061-3385 . . . . . . . . . . . . . . . . . . . . . . . . . . . .692 5061-3386 . . . . . . . . . . . . . . . . . . . . . . . . . . . .692 5061-3387 . . . . . . . . . . . . . . . . . . . . . . . . . . . .692 5061-3388 . . . . . . . . . . . . . . . . . . . . . . . . . . . .694 5061-3389 . . . . . . . . . . . . . . . . . . . . . . . . . . . .694 5061-3390 . . . . . . . . . . . . . . . . . . . . . . . . . . . .694 5061-3391 . . . . . . . . . . . . . . . . . . . . . . . . . . . .693 5061-3392 . . . . . . . . . . . . . . . . . . . . . . . . . . . .693 5061-3396 . . . . . . . . . . . . . . . . . . . . . . . . . . . .694 5061-3397 . . . . . . . . . . . . . . . . . . . . . . . . . . . .694 5061-3398 . . . . . . . . . . . . . . . . . . . . . . . . . . . .694 5061-5801 . . . . . . . . . . . . . . . . . . . . . . . . . . . .165 5061-5804 . . . . . . . . . . . . . . . . . . . . . . . . . . . .165 5061-5805 . . . . . . . . . . . . . . . . . . . . . . . . . . . .165 5061-5806 . . . . . . . . . . . . . . . . . . . . . . . . . . . .165 5061-5867 . . . . . . . . . . . . . . . . . . . . . . . . . . . .195 5061-5869 . . . . . . . . . . . . . . . . . . . .176, 188189 5061-5886 . . . . . . . . . . . . . . . . . . . . . . . .187, 195 5061-5889 . . . . . . . . . . . . . . . . . . . . . . . .195196 5061-5890 . . . . . . . . . . . . . . . . . . . . . . . .187, 195 5061-5891 . . . . . . . . . . . . . . . . . . . . . . . .187, 195 5061-5896 . . . . . . . . . . . . . . . . . . . . . . . .205, 214 5062-2405 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .38 5062-2418 . . . . . . . . . . . . . . . . . . . . . . . .447, 473 5062-2461 . . . . . . . . . . . . . . . . . . . . . . . . . . . .493 5062-2462 . . . . . . . . . . . . . . . . . . . .481, 485486 5062-2463 . . . . . . . . . . . . . . . . . . . .468, 481, 486 5062-2474 . . . . . . . . . . . . . . . . . . . . . . . . . . . .500 5062-2476 . . . . . . . . . . . . . . . . . . . . . . . .694, 697 5062-2477 . . . . . . . . . . . . . . . . . . . . . . . .692, 697 5062-2478 . . . . . . . . . . . . . . . . . . . . . . . . . . . .598 5062-2479 . . . . . . . . . . . . . . . . . . . . . . . . . . . .599 5062-2480 . . . . . . . . . . . . . . . . . . . . . . . . . . . .599 5062-2483 . . . . . . . . . . . . . . . . . . . . . . . .485486 5062-2484 . . . . . . . . . . . . . . . . . . . . . . . .462, 485 5062-2485 . . . . . . . . . . . . . . . . . . . .462463, 485 5062-2486 . . . . . . . . . . . . . . . . . . . . . . . . . . . .485 5062-2494 . . . . . . . . . . . . . . . . . . . . . . . .455, 494 5062-2495 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .38 5062-2496 . . . . . . . . . . . . . . . . . . . . . . . . . . . .693 5062-2497 . . . . . . . . . . . . . . . . . . . . . . . . . . . .693 5062-2500 . . . . . . . . . . . . . . . . . . . . . . . . . . . .599
Indices
731
Part Number Index

5062-3506 . . . . . . . . . . . . . . . . . . . . . . . .162, 196 5062-3507 . . . . . . . . . . . . . . . . . . . . . . . . . . . .162 5062-3508 . . . . . . . . . . . . . . . . . . . . . . . .162, 196 5062-3509 . . . . . . . . . . . . . . . . . . . . . . . . . . . .165 5062-3511 . . . . . . . . . . . . . . . . . . . . . . . . . . . .162 5062-3512 . . . . . . . . . . . . . . . . . . . . . . . . . . . .162 5062-3513 . . . . . . . . . . . . . . . . . . . . . . . . . . . .162 5062-3514 . . . . . . . . . . . . . . . . . . . .162, 179, 190 5062-3525 . . . . . . . . . . . . . . . . . . . . . . . .166, 177 5062-3538 . . . . . . . . . . . . . . . . . . . . . . . . . . . .162 5062-3549 . . . . . . . . . . . . . . . . . . . . . . . . . . . .165 5062-3550 . . . . . . . . . . . . . . . . . . . . . . . . . . . .164 5062-3551 . . . . . . . . . . . . . . . . . . . . . . . . . . . .164 5062-3552 . . . . . . . . . . . . . . . . . . . . . . . . . . . .164 5062-3553 . . . . . . . . . . . . . . . . . . . . . . . . . . . .164 5062-3554 . . . . . . . . . . . . . . . . . . . . . . . . . . . .164 5062-3555 . . . . . . . . . . . . . . . . . . . . . . . . . . . .164 5062-3556 . . . . . . . . . . . . . . . . . . . . . . . . . . . .164 5062-3560 . . . . . . . . . . . . . . . . . . . . . . . . . . . .102 5062-3561 . . . . . . . . . . . . . . . . . . . . . . . . . . . .102 5062-3580 . . . . . . . . . . . . . . . . . . . . . . . .162, 166 5062-3581 . . . . . . . . . . . . . . . . . . . . . . . .162, 166 5062-3587 . . . . . . . . . . . . . . . . . . . .157, 176, 189 5062-8515 . . . . . . . . . . . . . . . . . . . . . . . .485, 493 5062-8517 . . . . . . . . . . . . . . . . . . . .464, 486, 656 5062-8518 . . . . . . . . . . . . . . . . . . . . . . . . . . . .486 5062-8519 . . . . . . . . . . . . . . . . . . . . . . . . . . . .486 5062-8522 . . . . . . . . . . . . . . . . . . . .459, 474, 489 5062-8524 . . . . . . . . . . . . . . . . . . . .599, 666667 5062-8529 . . . . . . . . . . . . . . . . . . . . . . . .655, 694 5062-8530 . . . . . . . . . . . . . . . . . . . . . . . . . . . .499 5062-8534 . . . . . . . . . . . . . . . .147, 460, 486, 492 5062-8535 . . . . . . . . . . . . . . . . . . . . . . . .459, 489 5062-8541 . . . . . . . . . . . . . . . . . . . . . . . . . . . .447 5062-8544 . . . . . . . . . . . . . . . . . . . . . . . . . . . .656 5062-8553 . . . . . . . . . . . . . . . . . . . . . . . .478479 5062-8562 . . . . . . . . . . . . . . . . . . . . . . . .463, 485 5062-8571 . . . . . . . . . . . . . . . . . . . . . . . . . . . .654 5062-8572 . . . . . . . . . . . . . . . . . . . . . . . . . . . .654 5062-8573 . . . . . . . . . . . . . . . . . . . . . . . . . . . .654 5062-8574 . . . . . . . . . . . . . . . . . . . . . . . . . . . .654 5062-8575 . . . . . . . . . . . . . . . . . . . . . . . .653, 666 5062-8576 . . . . . . . . . . . . . . . . . . . .653, 666667 5062-8577 . . . . . . . . . . . . . . . . . . . . . . . . . . . .653 5062-8578 . . . . . . . . . . . . . . . . . . . .653, 666668 5062-8587 . . . . . . . . . . . . . . . . . . . . . . . .455, 467 5062-8588 . . . . . . . . . . . . . . . . . . . . . . . .471, 565 5062-9508 . . . . . . . . . . . . . . . . . . . . . . . . . . . .167 5062-9509 . . . . . . . . . . . . . . . . . . . . . . . . . . . .167 5062-9510 . . . . . . . . . . . . . . . . . . . . . . . . . . . .167 5062-9511 . . . . . . . . . . . . . . . . . . . . . . . . . . . .167 5063-5213 . . . . . . . . . . . . . . . . . . . . . . . . . . . .687 5063-5224 . . . . . . . . . . . . . . . . . . . . . . . . . . . .687 5063-5263 . . . . . . . . . . . . . . . . . . . . . . . .669, 679 5063-6502 . . . . . . . . . . . . . . . . . . . . . . . . . . . .450 5063-6503 . . . . . . . . . . . . . . . . . . . . . . . . .94, 699 5063-6505 . . . . . . . . . . . . . . . . . . . . . . . . . . . .463 5063-6506 . . . . . . . . . . . . . . . . . . . . . . . . . . . .467 5063-6510 5063-6511 5063-6512 5063-6513 5063-6514 5063-6515 5063-6516 5063-6519 5063-6520 5063-6521 5063-6523 5063-6524 5063-6526 5063-6529 5063-6531 5063-6535 5063-6536 5063-6537 5063-6538 5063-6540 5063-6541 5063-6542 5063-6543 5063-6546 5063-6547 5063-6548 5063-6549 5063-6550 5063-6551 5063-6552 5063-6553 5063-6554 5063-6556 5063-6557 5063-6558 5063-6559 5063-6560 5063-6561 5063-6562 5063-6563 5063-6564 5063-6565 5063-6566 5063-6570 5063-6571 5063-6572 5063-6573 5063-6574 5063-6575 5063-6577 5063-6586 5063-6588 5063-6589 5063-6591 5063-6592 5063-6593 5063-6597 5063-9153 . . . . . . . . . . . . . . . . . . . . . . . . . . . .667 . . . . . . . . . . . . . . . . . . . . . . . . . . . .666 . . . . . . . . . . . . . . . . . . . . . . . . . . . .666 . . . . . . . . . . . . . . . . . . . . . . . . . . . .666 . . . . . . . . . . . . . . . . . . . . . . . . .93, 658 . . . . . . . . . . . . . . . . . . . . . . . . . . . .658 . . . . . . . . . . . . . . . . . . . . . . . . . . . .597 . . . . . . . . . . . . . . . . . . . . . . . . . . . .513 . . . . . . . . . . . . . . . . . . . . . . . . .93, 658 . . . . . . . . . . . . . . . . . . . . . . . . .94, 699 . . . . . . . . . . . . . . . . . . . . . . . . .94, 699 . . . . . . . . . . . . . . . . . . . . . .9394, 699 . . . . . . . . . . . . . . . . . . . . . . . . . . . .471 . . . . . . . . . . . . . . . . . . . . . . . . . . . .597 . . . . . . . . . . . . . . . . . . . . . . . . . . . .464 . . . . . . . . . . . . . . . . . . . . . . . . . . . .666 . . . . . . . . . . . . . . . . . . . . . . . . . . . .666 . . . . . . . . . . . . . . . . . . . . . . . . . . . .666 . . . . . . . . . . . . . . . . . . . . . . . . . . . .666 . . . . . . . . . . . . . . . . . . . . . . . . . . . .666 . . . . . . . . . . . . . . . . . . . . . . . . . . . .666 . . . . . . . . . . . . . . . . . . . . . . . . . . . .666 . . . . . . . . . . . . . . . . . . . . . . . . . . . .666 . . . . . . . . . . . . . . . . . . . . . . . . . . . .692 . . . . . . . . . . . . . . . . . . . . . . . . . . . .692 . . . . . . . . . . . . . . . . . . . . . . . . . . . .692 . . . . . . . . . . . . . . . . . . . . . . . . . . . .692 . . . . . . . . . . . . . . . . . . . . . . . . . . . .692 . . . . . . . . . . . . . . . . . . . . . . . . . . . .692 . . . . . . . . . . . . . . . . . . . . . . . . . . . .692 . . . . . . . . . . . . . . . . . . . . . . . . . . . .692 . . . . . . . . . . . . . . . . . . . . . . . . . . . .692 . . . . . . . . . . . . . . . . . . . . . . . . . . . .692 . . . . . . . . . . . . . . . . . . . . . . . . . . . .692 . . . . . . . . . . . . . . . . . . . . . . . . . . . .693 . . . . . . . . . . . . . . . . . . . . . . . . . . . .693 . . . . . . . . . . . . . . . . . . . . . . . . . . . .693 . . . . . . . . . . . . . . . . . . . . . . . . . . . .693 . . . . . . . . . . . . . . . . . . . . . . . . . . . .693 . . . . . . . . . . . . . . . . . . . . . . . . . . . .693 . . . . . . . . . . . . . . . . . . . . . . . . . . . .693 . . . . . . . . . . . . . . . . . . . . . . . . . . . .693 . . . . . . . . . . . . . . . . . . . . . . . . . . . .693 . . . . . . . . . . . . . . . . . . . . . . . . . . . .694 . . . . . . . . . . . . . . . . . . . . . . . . . . . .694 . . . . . . . . . . . . . . . . . . . . . . . . . . . .694 . . . . . . . . . . . . . . . . . . . . . . . . . . . .694 . . . . . . . . . . . . . . . . . . . . . . . . . . . .513 . . . . . . . . . . . . . . . . . . . . . . . . . . . .698 . . . . . . . . . . . . . . . . . . . . . . . . . . . .694 . . . . . . . . . . . . . . . .455, 463, 467, 485 . . . . . . . . . . . . . . . . . . . . . . . . . . . .598 . . . . . . . . . . . .455, 463, 467, 485, 487 . . . . . . . . . . . . . . . . . . . . . . . . . . . .447 . . . . . . . . . . . . . . . . . . . . . . . . . . . .480 . . . . . . . . . . . . . . . . . . . . . . . .473, 480 . . . . . . . . . . . . . . . . . . . . . . . . .94, 481 . . . . . . . . . . . . . . . . . . . . . . . .678, 687 5064-8013 5064-8014 5064-8015 5064-8016 5064-8017 5064-8018 5064-8019 5064-8020 5064-8021 5064-8022 5064-8023 5064-8024 5064-8026 5064-8027 5064-8028 5064-8029 5064-8032 5064-8033 5064-8034 5064-8203 5064-8205 5064-8206 5064-8208 5064-8209 5064-8211 5064-8220 5064-8221 5064-8222 5064-8228 5064-8236 5064-8237 5064-8251 5064-8252 5064-8253 5064-8254 5064-8255 5064-8256 5064-8257 5064-8258 5064-8259 5064-8260 5064-8261 5064-8262 5064-8263 5064-8264 5064-8265 5064-8266 5064-8267 5064-8268 5064-8269 5064-8270 5064-8271 5064-8273 5064-8275 5064-8276 5064-8277 5064-8278 5064-8279 . . . . . . . . . . . . . . . . . . . . . . . .672, 681 . . . . . . . . . . . . . . . . . . . . . . . .671, 681 . . . . . . . . . . . . . . . . . . . . . . . .671, 681 . . . . . . . . . . . . . . . . . . . . . . . .671, 681 . . . . . . . . . . . . . . . . . . . . . . . .671, 681 . . . . . . . . . . . . . . . . . . . . . . . .671, 681 . . . . . . . . . . . . . . . . . . . . . . . .671, 681 . . . . . . . . . . . . . . . . . . . . . . . .671, 681 . . . . . . . . . . . . . . . . . . . . . . . .671, 681 . . . . . . . . . . . . . . . . . . . . . . . .671, 681 . . . . . . . . . . . . . . . . . . . . . . . .671, 681 . . . . . . . . . . . . . . . . . . . . . . . .671, 681 . . . . . . . . . . . . . . . . . . . . . . . .672, 681 . . . . . . . . . . . . . . . . . . . . . . . .672, 681 . . . . . . . . . . . . . . . . . . . . . . . .672, 681 . . . . . . . . . . . . . . . . . . . . . . . .672, 681 . . . . . . . . . . . . . . . . . . . . . . . .672, 681 . . . . . . . . . . . . . . . . . . . . . . . .672, 681 . . . . . . . . . . . . . . . . . . . . . . . .672, 681 . . . . . . . . . . . . . . . . . . . . . . . . . . . .667 . . . . . . . . . . . . . . . . . . . . . . . . . . . .667 . . . . . . . . . . . . . . . . . . . . . . . . . . . .667 . . . . . . . . . . . . . . . . . . . . . . . . . . . .667 . . . . . . . . . . . . . . . . . . . . . . . . . . . .667 . . . . . . . . . . . . . . . . . . . . . . . . . . . .458 . . . . . . . . . . . . . . . . . . . . . . . . .94, 482 . . . . . . . . . . . . . . . . . . . . . . . . . . . .147 . . . . . . . . . . . . . . . . . . . . . . . . . . . .147 . . . . . . . . . . . . . . . . . . . . . . . . . . . .668 . . . . . . . . . . . . . . . . . . . . . . . . . . . .668 . . . . . . . . . . . . . . . . . . . . . . . . . . . .668 . . . . . . . . . . . . . . . . . . . . . . . . . . . .578 . . . . . . . . . . . . . . . . . . . . . . . . . . . .578 . . . . . . . . . . . . . . . . . . . . . . . . . . . .529 . . . . . . . . . . . . . . . . . . . . . . . . . . . .529 . . . . . . . . . . . . . . . . . . . . . . . . . . . .529 . . . . . . . . . . . . . . . . . . . . . . . . . . . .529 . . . . . . . . . . . . . . . . . . . . . . . . . . . .529 . . . . . . . . . . . . . . . . . . . . . . . . . . . .529 . . . . . . . . . . . . . . . . . . . . . . . . . . . .534 . . . . . . . . . . . . . . . . . . . . . . . . . . . .529 . . . . . . . . . . . . . . . . . . . . . . . . . . . .529 . . . . . . . . . . . . . . . . . . . . . . . . . . . .529 . . . . . . . . . . . . . . . . . . . . . . . . . . . .534 . . . . . . . . . . . . . . . . . . . . . . . . . . . .534 . . . . . . . . . . . . . . . . . . . . . . . . . . . .534 . . . . . . . . . . . . . . . . . . . . . . . . . . . .534 . . . . . . . . . . . . . . . . . . . . . . . . . . . .534 . . . . . . . . . . . . . . . . . . . . . . . . . . . .534 . . . . . . . . . . . . . . . . . . . . . . . . . . . .540 . . . . . . . . . . . . . . . . . . . . . . . . . . . .534 . . . . . . . . . . . . . . . . . . . . . . . . . . . .540 . . . . . . . . . . . . . . . . . . . . . . . . . . . .464 . . . . . . . . . . . . . . . . . . . . . . . .561, 567 . . . . . . . . . . . . . . . . . . . . . . . .561, 567 . . . . . . . . . . . . . . . . . . . . . . . .561, 567 . . . . . . . . . . . . . . . . . . . . . . . .561, 567 . . . . . . . . . . . . . . . . . . . . . . . .561, 567
Indices
732
Part Number Index

5064-8280 . . . . . . . . . . . . . . . . . . . . . . . . . . . .579 5064-8281 . . . . . . . . . . . . . . . . . . . . . . . . . . . .579 5064-8282 . . . . . . . . . . . . . . . . . . . . . . . .561, 567 5064-8286 . . . . . . . . . . . . . . . . . . . . . . . . . . . .540 5064-8287 . . . . . . . . . . . . . . . . . . . . . . . . . . . .540 5064-8288 . . . . . . . . . . . . . . . . . . . . . . . . . . . .540 5064-8289 . . . . . . . . . . . . . . . . . . . . . . . .561, 567 5064-8290 . . . . . . . . . . . . . . . . . . . . . . . .561, 567 5064-8291 . . . . . . . . . . . . . . . . . . . . . . . . . . . .540 5064-8293 . . . . . . . . . . . . . . . . . . . . . . . .455, 469 5064-8294 . . . . . . . . . . . . . . . . . . . . . . . . . . . .534 5064-8295 . . . . . . . . . . . . . . . . . . . . . . . . . . . .534 5064-8296 . . . . . . . . . . . . . . . . . . . . . . . . . . . .540 5064-8297 . . . . . . . . . . . . . . . . . . . . . . . . . . . .540 5064-8298 . . . . . . . . . . . . . . . . . . . . . . . . . . . .540 5064-8299 . . . . . . . . . . . . . . . . . . . . . . . .561, 567 5064-8300 . . . . . . . . . . . . . . . . . . . . . . . . . . . .534 5065-4402 . . . . . . . . . . . . . . . . . . . . . . . .136, 469 5065-4410 . . . . . . . . . . . . . . . . . . . . . . . .469, 473 5065-4420 . . . . . . . . . . . . . . . . . . . . . . . . .94, 482 5065-4421 . . . . . . . . . . . . . . . . . . . . . . . . . . . .465 5065-4422 . . . . . . . . . . . . . . . . . . . . . . . .473, 480 5065-4423 . . . . . . . . . . . . . . . . . . . . . . . . . . . .473 5065-4426 . . . . . . . . . . . . . . . . . . . . . . . . . . . .447 5065-4427 . . . . . . . . . . . .529, 534, 540, 549, 561 5065-4445 . . . . . . . . . . . . . . . . . . . . . . . .465, 469 5065-4447 . . . . . . . . . . . . . . . . . . . . . . . . . . . .471 5065-4454 . . . . . . . . . . . . . . . . . . . . . . . . . . . .447 5065-4459 . . . . . . . . . . . . . . . . . . . . . . . . . . . .534 5065-4464 . . . . . . . . . . . . . . . . . . . . . . . . . . . .549 5065-4465 . . . . . . . . . . . . . . . . . . . . . . . .549, 561 5065-4466 . . . . . . . . . . . . . . . . . . . . . . . .549, 561 5065-4467 . . . . . . . . . . . . . . . . . . . . . . . .549, 561 5080-4978 . . . . . . . . . . . . . . . . . . . . . . . .183, 193 5080-5400 . . . . . . . . . . . . . . . . . . . . .91, 205, 214 5080-8707 . . . . . . . . . . . . . . . . . . . . . . . . . . . .153 5080-8716 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .99 5080-8723 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .99 5080-8726 . . . . . . . . . . . . . . . . . . . . . . . . . . . .153 5080-8728 . . . . . . . . . . . . . . . . . . . . . . . . . . . .153 5080-8732 . . . . . . . . . . . . . . . . . . . .180, 191192 5080-8735 . . . . . . . . . . . . . . . . . . . . . . . . . . . .103 5080-8745 . . . . . . . . . . . . . . . . . . . . . . . . . . . .153 5080-8750 . . . . . . . . . . . . . . . . . . . . . . .7172, 83 5080-8751 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .83 5080-8752 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .83 5080-8753 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .83 5080-8755 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .99 5080-8756 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .99 5080-8759 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .99 5080-8761 . . . . . . . . . . . . . . . . . . . . . . . . . . . .100 5080-8765 . . . . . . . . . . . . . . . . . . . . . . . . . . . .100 5080-8768 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .99 5080-8769 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .99 5080-8773 . . . . . . . . . . . .162, 185, 189, 193194 5080-8774 . . . . . . . .180, 185, 187, 191, 193195 5080-8778 . . . . . . . . . . . . . . . . . . . . . . . . . . . .103 5080-8800 . . . . . . . . . . . . . . . . . . . . . . . . . . . .103 5080-8846 . . . . . . . . . . . . . . . . . . . . . . . . . . . .195 5080-8853 . . .162, 178, 185, 189190, 193194, 197 5080-8858 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .97 5080-8872 . . . . . . . . . . . . . . . . . . . . . . . . . . . .193 5080-8894 . . . . . . . . . . . . . . . . . . . . . . . . . . . .153 5080-8896 . . . . . . . . . . . . . . . . . . . . . . . . . . . .153 5080-8898 . . . . . . . . . . . . . . . . . . . . . . . .180, 191 510100-114 . . . . . . . . . . . . . . . . . . . . . . . . . . .141 510100-134 . . . . . . . . . . . . . . . . . . . . . . . . . . .141 510100-150 . . . . . . . . . . . . . . . . . . . . . . . . . . .141 510100-160 . . . . . . . . . . . . . . . . . . . . . . . . . . .141 510100-170 . . . . . . . . . . . . . . . . . . . . . . . . . . .141 510200-214 . . . . . . . . . . . . . . . . . . . . . . . . . . .141 510200-234 . . . . . . . . . . . . . . . . . . . . . . . . . . .141 510200-250 . . . . . . . . . . . . . . . . . . . . . . . . . . .141 510200-260 . . . . . . . . . . . . . . . . . . . . . . . . . . .141 510200-270 . . . . . . . . . . . . . . . . . . . . . . . . . . .141 5180-0805 . . . . . . . . . . . . . . . . . . . . . . . . . . . .488 5180-0806 . . . . . . . . . . . . . . . . . . . . . . . . . . . .488 5180-0841 . . . . . . . . . . . . . . . . . . . . . . . .488, 496 5180-0842 . . . . . . . . . . . . . . . . . . . . . . . .488, 496 5180-0843 . . . . . . . . . . . . . . . . . . . . . . . . . . . .496 5180-0844 . . . . . . . . . . . . . . . . . . . . . . . .488, 496 5180-4102 . . . . . . . . . . . . . . . . . . . . .83, 196197 5180-4103 . . . . . . . . . . . . . .83, 85, 185, 188, 194 5180-4105 . . . .83, 85, 180, 185, 187, 191, 193194 5180-4108 . . . . . . . . . . . . . . . . . . . . .83, 447, 481 5180-4109 . . . . . . . . . . . . . . . . . . . . . .83, 85, 188 5180-4111 . . . . . . . . . . . . . . . . . . . . . . . . . .83, 85 5180-4114 . . . . . . . . . . . . . . . . . . . . .83, 447, 481 5180-4115 . . . . . . . . . . . . . . . . . . . . . .83, 85, 188 5180-4117 . . . . . . . . . . . . . . . . . . . . . . . . . .83, 85 5180-4120 . . . . . . . . . . . . . . . . . . . . . . . . . .83, 85 5180-4121 . . . . . . . . . . . . . . . . . . . .7172, 83, 85 5180-4124 . . . . . . . . . . . . . . . . . . . . . . . . . .83, 85 5180-4125 . . . . . . . . . . . . . . . . . . . . . . . . . .83, 85 5180-4127 . . . . . . . . . . . . . . . . . . . . . . . . . .8485 5180-4128 . . . . . . . . . . . . . . . . . . . . . . . . . .8485 5180-4129 . . . . . . . . . . . . . . . . . . . . . . . . . .8485 5180-4130 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .85 5180-4131 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .85 5180-4134 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .85 5180-4135 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .85 5180-4143 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .85 5180-4145 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .85 5180-4148 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .83 5180-4149 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .83 5180-4150 . . . . . . . . . . . . . . .182183, 192193 5180-4152 . . . . . . . . . . . . . . .182183, 192193 5180-4153 . . . . . . . . . . . . .91, 176, 182, 188, 192 5180-4160 . . . . . . . . . . . . . . . . . . . .7172, 8485 5180-4161 . . . . . . . . . . . . . . . . . . . . . . . . . .72, 85 5180-4162 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .86 5180-4168 . . . . . . . . . . . . . . . .158, 175, 188189 5180-4173 . . . . . . . . . . . . . . . .158, 175, 188189 5180-4181 . . . . . . . . . . . . . . . . . . . . . . . . .70, 189 5180-4182 . . . . . . .158, 175176, 188189, 195 5180-4196 . . . . . . . . . . . . . . . . . . . . . . .7172, 82 5181-1210 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .23 5181-1211 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .23 5181-1212 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .23 5181-1213 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .38 5181-1214 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .38 5181-1215 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .23 5181-1216 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .23 5181-1217 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .23 5181-1219 . . . . . . . . . . . . . . . . . . . . . . . . . . . .102 5181-1220 . . . . . . . . . . . . . . . . . . . . . . . . . . . .102 5181-1246 . . . . . . . . . . . . . . . . . . . . . . . . . . . .698 5181-1255 . . . . . . . . . . . . . . . . . . . . . . . . . . . .102 5181-1258 . . . . . . . . . . . . . . . . . . . . . . . . . . . .205 5181-1260 . . . . . . . . . . . . . . . . . . . . . . . . . . . .153 5181-1261 . . . . . . . . . . . . . . . . . . . . . . . . . . . .153 5181-1263 . . . . . . . . . . . . . . . . . . . . . . . . . . . .153 5181-1267 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .40 5181-1270 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .28 5181-1273 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .40 5181-1283 . . . . . . . . . . . . . . . . . . . . . . . . . . . .153 5181-1291 . . . . . . . . . . . . . . . . . . . . . . . . . . . .168 5181-1292 . . . . . . . . . . . . . . . . . . . . . . . . .83, 168 5181-1507 . . . . . . . . . . . . . . . . . . . . . . . . . . . .654 5181-1512 . . . . . . . . . . . . . . . . . . . . . . . . . . . .654 5181-1513 . . . . . . . . . . . . . . . . . . . . . . . . . . . .654 5181-1530 . . . . . . . . . . . . . . . . . . . . . . . .455, 478 5181-3308 . . . . . . . . . . . . . . . . . . . . . . . . . . . .162 5181-3311 . . . . . . . . . . . . . . . . . . . . . . . .182, 192 5181-3315 . . . . . . . . . . . . . . . . . . . . . . . . . . . .157 5181-3316 . . . . . . . . . . . . . . . . . . . . . . . .157, 176 5181-3317 . . . . . . . . . . . . . . . . . . . . . . . . . . . .189 5181-3319 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .40 5181-3321 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .40 5181-3322 . . . . . . . . . . . . . . . . . . . . . . . . . . . .162 5181-3323 . . . . . . . . . . . . . . . . . . . .162, 179, 190 5181-3351 . . . . . . . . . . . . . . . . . . . . . . . . . . . .291 5181-3352 . . . . . . . . . . . . . . . . . . . . . . . . . . . .291 5181-3353 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .95 5181-3354 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .40 5181-3356 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .40 5181-3358 . . . . . . . . . . . . . . . . . . . . . . . . . .4041 5181-3360 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .40 5181-3361 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .40 5181-3365 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .40 5181-3366 . . . . . . . . . . . . . . . . . . . . . . . . . . . .215 5181-3367 . . . . . . . . . . . . . . . . . . . . . . . . . . . .215 5181-3375 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .20 5181-3376 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .20 5181-3377 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .28 5181-3382 . . . . . . . . . . . . . . . . . . . .180, 191192 5181-3383 . . . . . . . . . . . . . . . . . . . . . . . . . . . .153 5181-3388 . . . . . . . . . . . . . . . . . . . . . . . . . . . .166 5181-3389 . . . . . . . . . . . . . . . . . . . . . . . . . . . .166 5181-3390 . . . . . . . . . . . . . . . . . . . . . . . . . . . .166 5181-3391 . . . . . . . . . . . . . . . . . . . . . . . . . . . .166 5181-3392 . . . . . . . . . . . . . . . . . . . . . . . . . . . .166 5181-3393 . . . . . . . . . . . . . . . . . . . . . . . . . . . .166 5181-3394 . . . . . . . . . . . . . . . . . . . . . . . . .70, 189
Indices
733
Part Number Index

5181-3395 5181-3396 5181-3397 5181-3398 5181-3400 5181-7459 5181-7460 5181-8801 5181-8806 5181-8808 5181-8809 5181-8810 5181-8811 5181-8813 5181-8815 5181-8816 5181-8818 5181-8822 5181-8827 5181-8830 5181-8833 5181-8836 5181-8837 5181-8838 5181-8839 5181-8863 5181-8866 5181-8872 5182-0540 5182-0541 5182-0542 5182-0543 5182-0544 5182-0545 5182-0546 5182-0547 5182-0548 5182-0549 5182-0550 5182-0551 5182-0552 5182-0553 5182-0554 5182-0555 5182-0556 5182-0557 5182-0558 5182-0566 5182-0567 5182-0714 5182-0715 5182-0716 5182-0717 5182-0718 5182-0719 5182-0720 5182-0721 . . . . . . . . . . . . . . . . . . . . . . . . . . . .165 . . . . . . . . . . . . . . . . . . . .165, 179, 190 . . . . . . . . . . . . . . . . . . . . . . . . . . . .165 . . . . . . . . . . . . . . . . . . . . . . . . . . . .165 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .23 . . . . . . . . . . . . . . . . . . . . . . . . . . . .168 . . . . . . . . . . . . . . . . . . . . . . . . . . . .168 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .23 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .41 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .41 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .41 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .40 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .41 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .41 . . . . . . . . . . . . . . . . . . . . . . . . . . . .154 . . . . . . . . . . . . . . . . . . . . . . . . . . . .154 . . . . . . . . . . . . . . . . . . . .157, 176, 189 . . . . . . . . . . . . . . . . . . . . . . . . . . . .172 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .38 . . . . . . .163, 176, 178180, 185, 189, 193194, 201 . . . . . . . . . . . . . . . . . . . . . . . .154, 190 . . . . . . . . . . . . . . . . . . . . .88, 189, 314 . . . . . . . . . . . . . . . . . . . . . . . . . . . .191 . . . . . . . . . . . . . . . . . . . . . . . .176, 189 . . . . . . . . . . . . . . . . . . . . . . . . . . . .154 . . . . . . . . . . . . . . . . . . . . . . . . . . . .205 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .42 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .28 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .26 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .26 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .26 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .20 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .24 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .24 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .24 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .27 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .27 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .28 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .26 . . . . . . . . . . . . . . . . . . . . . . . . .32, 173 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .23 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .19 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .19 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .19 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .19 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .19 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .19 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .26 . . . . . . . . . . . . . . . . . . . . . .22, 25, 654 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .15 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .15 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .15 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .16 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .16 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .16 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .16 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .16 5182-0722 5182-0723 5182-0724 5182-0725 5182-0726 5182-0727 5182-0728 5182-0729 5182-0730 5182-0731 5182-0732 5182-0733 5182-0734 5182-0735 5182-0736 5182-0737 5182-0740 5182-0741 5182-0742 5182-0743 5182-0744 5182-0746 5182-0771 5182-0772 5182-0773 5182-0774 5182-0775 5182-0778 5182-0779 5182-0780 5182-0781 5182-0783 5182-0788 5182-0789 5182-0792 5182-0793 5182-0794 5182-0795 5182-0796 5182-0815 5182-0816 5182-0817 5182-0818 5182-0820 5182-0821 5182-0830 5182-0831 5182-0832 5182-0833 5182-0834 5182-0835 5182-0836 5182-0837 5182-0838 5182-0839 5182-0840 5182-0842 5182-0844 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .16 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .16 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .16 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .16 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .17 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .17 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .17 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .17 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .17 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .17 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .18 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .18 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .18 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .18 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .18 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .18 . . . . . . . . . . . . . . . . . . . . . . . . . . . .164 . . . . . . . . . . . . . . . . . . . . . . . . . . . .164 . . . . . . . . . . . . . . . . . . . . . . . . . . . .164 . . . . . . . . . . . . . . . . . . . . . . . . . . . .164 . . . . . . . . . . . . . . . . . . . . . . . . . . . .164 . . . . . . . . . . . . . . . . . . . . . . . . . . . .170 . . . . . . . . . . . . . . . . . . . . . . . . . . . .171 . . . . . . . . . . . . . . . . . . . . . . . . . . . .171 . . . . . . . . . . . . . . . . . . . . . . . . . . . .171 . . . . . . . . . . . . . . . . . . . . . . . . . . . .171 . . . . . . . . . . . . . . . . . . . . . . . . . . . .171 . . . . . . . . . . . . . . . . . . . . . . . . . . . .171 . . . . . . . . . . . . . . . . . . . . . . . . . . . .171 . . . . . . . . . . . . . . . . . . . . . . . . . . . .171 . . . . . . . . . . . . . . . . . . . . . . . . . . . .171 . . . . . . . . . . . . . . . . . . . . . . . . . . . .171 . . . . . . . . . . . . . . . . . . . . . . . . . . . .170 . . . . . . . . . . . . . . . . . . . . . . . . . . . .170 . . . . . . . . . . . . . . . . . . . . . . . . . . . .170 . . . . . . . . . . . . . . . . . . . . . . . . . . . .170 . . . . . . . . . . . . . . . . . . . . . . . . . . . .170 . . . . . . . . . . . . . . . . . . . . . . . . . . . .170 . . . . . . . . . . . . . . . . . . . . . . . . . . . .170 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .69 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .68 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .69 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .69 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .69 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .69 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .41 . . . . . . . . . . . . . . . . . . . . . . . . .42, 179 . . . . . . . . . . . . . . . . . . . . . .40, 42, 179 . . . . . . . . . . . . . . . . . . . . . . . . .42, 179 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .41 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .40 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .42 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .33 . . . . . . . . . . . . . . . . . . . . . . . . .33, 175 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .35 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .35 . . . . . . . . . . . . . . . . . . . . . . . . . . . .108 . . . . . . . . . . . . . . . . . . . . . . . . . . . .170 5182-0845 5182-0846 5182-0847 5182-0848 5182-0849 5182-0850 5182-0851 5182-0852 5182-0853 5182-0864 5182-0865 5182-0866 5182-0867 5182-0868 5182-0869 5182-0870 5182-0871 5182-0875 5182-0893 5182-0894 5182-0895 5182-3423 5182-3428 5182-3442 5182-3444 5182-3445 5182-3449 5182-3450 5182-3453 5182-3454 5182-3457 5182-3458 5182-3459 5182-3465 5182-3466 5182-3477 5182-3495 5182-3496 5182-3498 5182-3499 5182-9604 5182-9605 5182-9606 5182-9608 5182-9609 5182-9610 5182-9612 5182-9613 5182-9615 5182-9619 5182-9620 5182-9621 5182-9622 5182-9623 5182-9624 5182-9625 5182-9626 5182-9627 . . . . . . . . . . . . . . . . . . . . . . . . . . . .170 . . . . . . . . . . . . . . . . . . . . . . . . . . . .170 . . . . . . . . . . . . . . . . . . . . . . . . . . . .170 . . . . . . . . . . . . . . . . . . . . . . . . . . . .170 . . . . . . . . . . . . . . . . . . . . . . . . . . . .170 . . . . . . . . . . . . . . . . . . . . . . . . . . . .170 . . . . . . . . . . . . . . . . . . . . . . . . . . . .170 . . . . . . . . . . . . . . . . . . . . . . . . . . . .170 . . . . . . . . . . . . . . . . . . . . . . . . . . . .154 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .19 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .19 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .19 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .18 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .18 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .18 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .26 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .23 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .41 . . . . . . . . . . . . . . . . . . . . . . . . . . . .170 . . . . . . . . . . . . . . . . . . . . . . . . . . . .170 . . . . . . . . . . . . . . . . . . . . . . . . . . . .170 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .69 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .43 . . . . . . . . . . . . . . . . . . . . . . . . . . . .154 . . . . . . . . . . . . . . . . . . . . . . . . . . . .154 . . . . . . . . . . . . . . . . . . . . . . . . . . . .154 . . . . . . . . . . . . . . . . . . . . . . . . . . . .189 . . . . . . . . . . . . . . . . . . . . . . . .182, 192 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .72 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .21 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .26 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .26 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .26 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .71 . . . . . . . . . . . . . . . . . . . . . . . . .71, 205 . . . . . . . . . . . . . . . . . . . . . . . . . . . .186 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .44 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .44 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .44 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .43 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .44 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .43 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .44 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .44 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .44 . . . . . . . . . . . . . . . . . . . . . . . . .44, 484 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .44 . . . . . . . . . . . . . . . . . . . . . . . . .46, 454 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .43 . . . . . . . . . . . . . . . . . . . . . . . . .46, 454 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .46 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .44 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .42 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .43 . . . . . . . . . . . . . . . . . . . . . . . . .46, 454 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .43 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .42 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .43
Indices
734
Part Number Index

5182-9628 5182-9629 5182-9630 5182-9633 5182-9635 5182-9636 5182-9638 5182-9639 5182-9640 5182-9641 5182-9642 5182-9643 5182-9644 5182-9645 5182-9646 5182-9648 5182-9651 5182-9652 5182-9658 5182-9661 5182-9671 5182-9673 5182-9676 5182-9677 5182-9679 5182-9683 5182-9689 5182-9697 5182-9703 5182-9704 5182-9705 5182-9706 5182-9707 5182-9709 5182-9710 5182-9711 5182-9712 5182-9713 5182-9719 5182-9720 5182-9722 5182-9725 5182-9733 5182-9734 5182-9747 5182-9748 5182-9749 5182-9754 5182-9756 5182-9757 5182-9758 5182-9759 5182-9760 5182-9761 5182-9762 5182-9763 5182-9765 5182-9768 . . . . . . . . . . . . . . . . . . . . . . . . .46, 454 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .44 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .46 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .45 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .43 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .43 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .44 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .46 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .43 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .43 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .44 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .44 . . . . . . . . . . . . . . . . . . . . . . . . .46, 454 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .45 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .80 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .80 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .43 . . . . . . . . . . . . . . . . . . . .164, 176, 188 . . . . . . . . . . . . . . . . . . . . . . . . .46, 454 . . . . . . . . . . . . . . . . . . . . . . . . . .44, 46 . . . . . . . . . . . . . . . . . . . . . . . . . .44, 46 . . . . . . . . . . . . . . . . . . . . . . . . . . . .186 . . . . . . . . . . . . . . . . . . . . . . . . . . . .186 . . . . . . . . . . . . . . . . . . . . . . . . . . . .186 . . . . . . . . . . . . . . . . . . . . . . . . . . . .186 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .43 . . . . . . . . . . . . . . . . . . . . . . . . . . . .172 . . . . . . . . . . . . . . . . . . . . . . . . . . . .654 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .68 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .68 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .69 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .69 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .69 . . . . . . . . . . . . . . . . . . . . . . . . . .44, 46 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .46 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .46 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .43 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .69 . . . . . . . . . . . . . . . . . . . . . . . .454, 486 . . . . . . . . . . . . . . . . . . . . . . . . . . . .454 . . . . . . . . . . . . . . . . . . . . . . . . . . . .184 . . . . . . . . . . . . . . . . . . . . . . . . . . . .454 . . . . . . . . . . . . . . . . . . . . . . . . .98, 175 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .42 . . . . . . . . . . . . . . . . . . . . . . . . . . . .177 . . . . . . . . . . . . . . . . . . . . . . . . . . . .177 . . . . . . . . . . . . . . . . . . . . . . . . . . . .177 . . . . . . . . . . . . . . . . . . . . . . . . . . . .177 . . . . . . . . . . . . . . . . . . . . . . . . . . . .177 . . . . . . . . . . . . . . . . . . . . . . . . . . . .177 . . . . . . . . . . . . . . . . . . . . . . . . . . . .177 . . . . . . . . . . . . . . . . . . . . . . . . . . . .177 . . . . . . . . . . . . . . . . . . . . . . . . . . . .177 . . . . . . . . . . . . . . . . . . . . . . . . . . . .177 . . . . . . . . . . . . . . . . . . . . . . . . . . . .177 . . . . . . . . . . . . . . . . . . . . . . . . . . . .177 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .91 . . . . . . . . . . . . . . . . . . . . . . . . . . . .177 5182-9769 5182-9770 5182-9775 5182-9776 5182-9777 5182-9778 5182-9779 5182-9780 5182-9791 5182-9792 5182-9793 5182-9795 5182-9799 5182-9800 5183-0314 5183-0316 5183-0318 5183-0379 5183-1907 5183-1941 5183-2005 5183-2006 5183-2007 5183-2008 5183-2009 5183-2021 5183-2022 5183-2030 5183-2036 5183-2037 5183-2038 5183-2041 5183-2042 5183-2058 5183-2067 5183-2068 5183-2069 5183-2070 5183-2071 5183-2072 5183-2073 5183-2074 5183-2075 5183-2076 5183-2077 5183-2078 5183-2079 5183-2080 5183-2081 5183-2082 5183-2083 5183-2085 5183-2086 5183-2087 5183-2088 5183-2089 5183-2090 5183-2096 . . . . . . . . . . . . . . . . . . . . . . . . . . . .177 . . . . . . . . . . . . . . . . . . . . . . . . . . . .177 . . . . . . . . . . . . . . . . . . . . . . . . . . . .177 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .64 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .64 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .64 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .64 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .64 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .64 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .64 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .64 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .64 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .42 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .42 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .40 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .41 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .41 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .98 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .64 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .82 . . . . . . . . . . . . . . . . . . . . . . . . . . . .109 . . . . . . . . . . . . . . . . . . . . . . . . . . . .109 . . . . . . . . . . . . . . . . . . . . . . . . . . . .184 . . . . . . . . . . . . . . . . . . . . . . . . . . . .108 . . . . . . . . . . . . . . . . . . . . . . . . . . . .102 . . . . . . . . . . . . . . . . . . . . . . . . . . . .597 . . . . . . . . . . . . . . . . . . . . . . . . . . . .597 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .16 . . . . . . . . . . . . . . . . . . . . . . . . . . . .177 . . . . . . . . . . . . . . . . . . . . . . . . . . . .177 . . . . . . . . . . . . . . . . . . . . . . . . . . . .177 . . . . . . . . . . . . . . . . . . . . . . . . . . . .177 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .40 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .41 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .15 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .15 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .15 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .15 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .15 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .15 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .16 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .17 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .17 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .16 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .16 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .16 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .18 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .18 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .18 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .19 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .19 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .28 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .28 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .28 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .31 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .31 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .31 . . . . . . . . . . . . . . . . . . . . . . . . . . . .206 5183-4428 5183-4429 5183-4432 5183-4433 5183-4436 5183-4437 5183-4438 5183-4442 5183-4448 5183-4450 5183-4459 5183-4460 5183-4461 5183-4464 5183-4474 5183-4475 5183-4476 5183-4477 5183-4478 5183-4479 5183-4480 5183-4491 5183-4492 5183-4493 5183-4494 5183-4495 5183-4496 5183-4497 5183-4498 5183-4499 5183-4500 5183-4504 5183-4505 5183-4506 5183-4507 5183-4508 5183-4509 5183-4510 5183-4511 5183-4519 5183-4521 5183-4523 5183-4524 5183-4526 5183-4528 5183-4530 5183-4532 5183-4534 5183-4536 5183-4537 5183-4538 5183-4539 5183-4540 5183-4547 5183-4549 5183-4550 5183-4551 5183-4552 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .29 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .29 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .29 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .29 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .30 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .30 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .30 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .30 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .32 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .32 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .32 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .32 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .32 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .32 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .33 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .33 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .35 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .34 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .34 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .34 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .34 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .20 . . . . . . . . . . . . . . . . . . . . . . . . . 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. . . . . . . . . . . . . . . . . . . . . .45 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .45
Indices
735
Part Number Index

5183-4553 5183-4554 5183-4598 5183-4599 5183-4600 5183-4606 5183-4607 5183-4608 5183-4613 5183-4614 5183-4619 5183-4620 5183-4621 5183-4623 5183-4624 5183-4625 5183-4626 5183-4627 5183-4641 5183-4642 5183-4643 5183-4644 5183-4645 5183-4647 5183-4649 5183-4654 5183-4655 5183-4656 5183-4657 5183-4658 5183-4659 5183-4660 5183-4669 5183-4670 5183-4680 5183-4681 5183-4682 5183-4687 5183-4688 5183-4691 5183-4692 5183-4693 5183-4694 5183-4695 5183-4696 5183-4697 5183-4698 5183-4699 5183-4700 5183-4701 5183-4702 5183-4703 5183-4704 5183-4705 5183-4706 5183-4707 5183-4708 5183-4709 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .45 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .45 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .64 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .64 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .64 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .46 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .64 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .64 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .46 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .46 . . . . . . . . . . . . . . . . . . . . . . . . . . . .654 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .88 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .88 . . . . . . . . . . . . . . . . . . . . . . . . . . . .654 . . . . . . . . . . . . . . . . . . . . . . . . . . . .596 . . . . . . . . . . . . . . . . . . . . . . . . . . . .596 . . . . . . . . . . . . . . . . . . . . . . . . . . . .596 . . . . . . . . . . . . . . . . . . . . . . . . . . . .596 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .81 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .81 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .81 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .81 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .81 . . . . . . . . . . . . . . . . . . . . . . . .157, 176 . . . . . . . . . . . . . . . . . . . . . . . . . . . .106 . . . . . . . . . . . . . . . . . . . . . . . . . . . .107 . . . . . . . . . . . . . . . . . . . . . . . . . . . .107 . . . . . . . . . . . . . . . . . . . . . . . . . . . .107 . . . . . . . . . . . . . . . . . . . . . . . . . . . .107 . . . . . . . . . . . . . . . . . . . . . . . . . . . .107 . . . . . . . . . . . . . . . . . . . . . . . . . . . .107 . . . . . . . . . . . . . . . . . . . . . . . . . . . .107 . . . . . . . . . . . . . . . . . . . . . . . . . . . .656 . . . . . . . . . . . . . . . . . . . . . . . . . . . .656 . . . . . . . . . . . . . . . . . . . . . . . . . . . .690 . . . . . . . . . . . . . . . . . . . . . . . . . . . .690 . . . . . . . . . . . . . . . . . . . . . . . . . . . .690 . . . . . . . . . . . . . . . . . . . . . . . . . . . .690 . . . . . . . . . . . . . . . . . . . . . . . . . . . .690 . . . . . . . . . . . . . . . . . . . . . . . . . . . .157 . . . . . . . . . . . . . . . . . . . . . . . . . . . .157 . . . . . . . . . . . . . . . . . . . . . . . . . . . .157 . . . . . . . . . . . . . . . . . . . . . . . . . . . .157 . . . . . . . . . . . . . . . . . . . . . . . . . . . .157 . . . . . . . . . . . . . . . . . . . . . . . .157, 176 . . . . . . . . . . . . . . . . . . . . . . . . . . . .157 . . . . . . . . . . . . . . . . . . . . . . . . . . . .157 . . . . . . . . . . . . . . . . . . . . . . . . . . . .157 . . . . . . . . . . . . . . . . . . . . . . . . . . . .157 . . . . . . . . . . . . . . . . . . . . . . . . . . . .157 . . . . . . . . . . . . . . . . . . . . . . . .157, 176 . . . . . . . . . . . . . . . . . . . . . . . . . . . .157 . . . . . . . . . . . . . . . . . . . . . . . . . . . .157 . . . . . . . . . . . . . . . . . . . . . . . . . . . .157 . . . . . . . . . . . . . . . . . . . . . . . . . . . .157 . . . . . . . . . . . . . . . . . . . . . . . . . . . .157 . . . . . . . . . . . . . . . . . . . . . . . . . . . .157 . . . . . . . . . . . . . . . . . . . . . . . . . . . .157 5183-4710 . . . . . . . . . . . . . . . . . . . . . . . . . . . .157 5183-4711 . . . . . . . . . . . . . . . . . . . . . . . . . . . .157 5183-4712 . . . . . . . . . . . . . . . . . . . . . . . . . . . .157 5183-4713 . . . . . . . . . . . . . . . . . . . . . . . . . . . .157 5183-4727 . . . . . . . . . . . . . . . . . . . . . . . . . . . .107 5183-4728 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .41 5183-4729 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .41 5183-4730 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .41 5183-4731 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .25 5183-4732 . . . . . . . . . . . . . . . . . . . .163, 176, 185 5183-4733 . . . . . . . . . . . . . . . . . . . . . . . . . . . .172 5183-4734 . . . . . . . . . . . . . . . . . . . . . . . . . . . .172 5183-4735 . . . . . . . . . . . . . . . . . . . . . . . . . . . .172 5183-4736 . . . . . . . . . . . . . . . . . . . . . . . . . . . .172 5183-4737 . . . . . . . . . . . . . . . . . . . . . . . . . . . .172 5183-4738 . . . . . . . . . . . . . . . . . . . . . . . . . . . .172 5183-4744 . . . . . . . . . . . . . . . . . . . . . . . . . . . .172 5183-4745 . . . . . . . . . . . . . . . . . . . . . . . . . . . .172 5183-4746 . . . . . . . . . . . . . . . . . . . . . . . . . . . .172 5183-4757 . . . . . . . .151, 175176, 180, 189, 191 5183-4758 . . . . . . . . . . . . . . . . . . . .151, 178, 190 5183-4759 . . . . . . . . . . . . . . . . . . . . . . . . . . . .152 5183-4760 . . . . . . . . . . . . . . . . . . . .152, 178, 190 5183-4761 . . . . . . . . . . . . . . . . . . . . . . . . . . . .151 5183-4762 . . . . . . . . . . . . . . . . . . . . . . . . . . . .151 5183-4763 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .37 5183-4769 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .69 5183-4770 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .69 5183-4771 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .69 5183-4774 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .69 5183-4779 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .75 5183-4780 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .75 5183-4781 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .75 5183-4782 . . . . . . . . . . . . . . . . . . . . . . . . . . . .212 5183-4783 . . . . . . . . . . . . . . . . . . . . . . . . . . . .212 5183-4785 . . . . . . . . . . . . . . . . . . . . . . . . . . . .212 5183-4786 . . . . . . . . . . . . . . . . . . . . . . . . . . . .212 5183-4787 . . . . . . . . . . . . . . . . . . . . . . . . . . . .212 5183-4788 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .75 5183-4789 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .91 5183-4799 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .37 5184-3539 . . . . . . . . . . . . . . . . . . . . . . . . . . . .100 5184-3540 . . . . . . . . . . . . . . . . . . . . . . . . . . . .100 5184-3541 . . . . . . . . . . . . . . . . . . . . . . . . . . . .100 5184-3542 . . . . . . . . . . . . . . . . . . . . . . . . . . . .100 5184-3543 . . . . . . . . . . . . . . . . . . . . . . . . . . . .100 5184-3544 . . . . . . . . . . . . . . . . . . . . . . . . . . . .101 5184-3545 . . . . . . . . . . . . . . . . . . . . . . . . . . . .101 5184-3546 . . . . . . . . . . . . . . . . . . . . . . . . . . . .101 5184-3547 . . . . . . . . . . . . . . . . . . . . . . . . . . . .101 5184-3548 . . . . . . . . . . . . . . . . . . . . . . . . . . . .101 5184-3550 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .16 5184-3551 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .21 5184-3552 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .25 5184-3554 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .16 5184-3555 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .21 5184-3556 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .25 5184-3563 . . . . . . . . . . . . . . . . . . . . . . . . . . . .689 5184-3564 5184-3565 5184-3566 5184-3567 5184-3569 5184-3570 5184-3571 5184-3572 5184-3573 5184-3574 5184-3575 5184-3576 5184-3577 5184-3578 5184-3579 5184-3580 5184-3588 5184-3589 5184-3590 5184-3591 5184-3592 5184-3593 5184-3594 5184-3595 5184-3596 5184-3597 5184-3598 5184-3599 5184-3600 5185-5701 5185-5702 5185-5703 5185-5704 5185-5705 5185-5706 5185-5707 5185-5708 5185-5709 5185-5710 5185-5711 5185-5712 5185-5713 5185-5714 5185-5721 5185-5722 5185-5723 5185-5724 5185-5725 5185-5726 5185-5727 5185-5728 5185-5729 5185-5730 5185-5731 5185-5732 5185-5733 5185-5734 5185-5735 . . . . . . . . . . . . . . . . . . . . . . . . . . . .689 . . . . . . . . . . . . . . . . . . . . . . . . . . . .689 . . . . . . . . . . . . . . . . . . . . . . . . . . . .689 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .37 . . . . . . . . . . . . . . . . . . . . . . . . . . . .212 . . . . . . . . . . . . . . . . . . . . . . . . . . . .212 . . . . . . . . . . . . . . . . . . . . . . . . . . . .212 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .37 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .37 . . . . . . . . . . . . . . . . . . . . . . . . . . . .118 . . . . . . . . . . . . . . . . . . . . . . . . . . . .118 . . . . . . . . . . . . . . . . . . . . . . . . . . . .118 . . . . . . . . . . . . . . . . . . . . . . . . . . . .118 . . . . . . . . . . . . . . . . . . . . . . . . . . . .118 . . . . . . . . . . . . . . . . . . . . . . . . . . . .118 . . . . . . . . . . . . . . . . . . . . . . . . . . . .118 . . . . . . . . . . . . . . . . . . . . . . . . . . . .114 . . . . . . . . . . . . . . . . . . . . . . . . . . . .114 . . . . . . . . . . . . . . . . . . . . . . . . . . . .114 . . . . . . . . . . . . . . . . . . . . . . . . . . . .114 . . . . . . . . . . . . . . . . . . . . . . . . . . . .114 . . . . . . . . . . . . . . . . . . . . . . . . . . . .114 . . . . . . . . . . . . . . . . . . . . . . . . . . . .114 . . . . . . . . . . . . . . . . . . . . . . . . . . . .116 . . . . . . . . . . . . . . . . . . . . . . . . . . . .116 . . . . . . . . . . . . . . . . . . . . . . . . . . . .116 . . . . . . . . . . . . . . . . . . . . . . . . . . . .116 . . . . . . . . . . . . . . . . . . . . . . . . . . . .116 . . . . . . . . . . . . . . . . . . . . . . . . . . . .116 . . . . . . . . . . . . . . . . . . . . . . . . . . . .116 . . . . . . . . . . . . . . . . . . . . . . . . . . . .119 . . . . . . . . . . . . . . . . . . . . . . . . . . . .119 . . . . . . . . . . . . . . . . . . . . . . . . . . . .119 . . . . . . . . . . . . . . . . . . . . . . . . . . . .119 . . . . . . . . . . . . . . . . . . . . . . . . . . . .119 . . . . . . . . . . . . . . . . . . . . . . . . . . . .119 . . . . . . . . . . . . . . . . . . . . . . . . . . . .119 . . . . . . . . . . . . . . . . . . . . . . . . . . . .118 . . . . . . . . . . . . . . . . . . . . . . . . . . . .118 . . . . . . . . . . . . . . . . . . . . . . . . . . . .118 . . . . . . . . . . . . . . . . . . . . . . . . . . . .118 . . . . . . . . . . . . . . . . . . . . . . . . . . . .118 . . . . . . . . . . . . . . . . . . . . . . . . . . . .118 . . . . . . . . . . . . . . . . . . . . . . . . . . . .114 . . . . . . . . . . . . . . . . . . . . . . . . . . . .114 . . . . . . . . . . . . . . . . . . . . . . . . . . . .114 . . . . . . . . . . . . . . . . . . . . . . . . . . . .114 . . . . . . . . . . . . . . . . . . . . . . . . . . . .114 . . . . . . . . . . . . . . . . . . . . . . . . . . . .114 . . . . . . . . . . . . . . . . . . . . . . . . . . . .116 . . . . . . . . . . . . . . . . . . . . . . . . . . . .116 . . . . . . . . . . . . . . . . . . . . . . . . . . . .116 . . . . . . . . . . . . . . . . . . . . . . . . . . . .116 . . . . . . . . . . . . . . . . . . . . . . . . . . . .116 . . . . . . . . . . . . . . . . . . . . . . . . . . . .116 . . . . . . . . . . . . . . . . . . . . . . . . . . . .119 . . . . . . . . . . . . . . . . . . . . . . . . . . . .119 . . . . . . . . . . . . . . . . . . . . . . . . . . . .119
Indices
736
Part Number Index

5185-5736 5185-5737 5185-5738 5185-5739 5185-5740 5185-5741 5185-5745 5185-5746 5185-5747 5185-5748 5185-5749 5185-5750 5185-5751 5185-5752 5185-5753 5185-5754 5185-5755 5185-5756 5185-5757 5185-5758 5185-5759 5185-5760 5185-5761 5185-5762 5185-5763 5185-5764 5185-5765 5185-5766 5185-5767 5185-5768 5185-5769 5185-5770 5185-5771 5185-5772 5185-5773 5185-5774 5185-5775 5185-5778 5185-5779 5185-5780 5185-5781 5185-5782 5185-5783 5185-5784 5185-5785 5185-5786 5185-5787 5185-5788 5185-5789 5185-5790 5185-5791 5185-5792 5185-5793 5185-5794 5185-5795 5185-5796 5185-5797 5185-5798 . . . . . . . . . . . . . . . . . . . . . . . . . . . .119 . . . . . . . . . . . . . . . . . . . . . . . . . . . .119 . . . . . . . . . . . . . . . . . . . . . . . . . . . .119 . . . . . . . . . . . . . . . . . . . . . . . . . . . .118 . . . . . . . . . . . . . . . . . . . . . . . . . . . .118 . . . . . . . . . . . . . . . . . . . . . . . . . . . .118 . . . . . . . . . . . . . . . . . . . . . . . . . . . .114 . . . . . . . . . . . . . . . . . . . . . . . . . . . .114 . . . . . . . . . . . . . . . . . . . . . . . . . . . .114 . . . . . . . . . . . . . . . . . . . . . . . . . . . .116 . . . . . . . . . . . . . . . . . . . . . . . . . . . .116 . . . . . . . . . . . . . . . . . . . . . . . . . . . .116 . . . . . . . . . . . . . . . . . . . . . . . . . . . .119 . . . . . . . . . . . . . . . . . . . . . . . . . . . .119 . . . . . . . . . . . . . . . . . . . . . . . . . . . .119 . . . . . . . . . . . . . . . . . . . . . . . . . . . .133 . . . . . . . . . . . . . . . . . . . . . . . . . . . .133 . . . . . . . . . . . . . . . . . . . . . . . . . . . .133 . . . . . . . . . . . . . . . . . . . . . . . . . . . .133 . . . . . . . . . . . . . . . . . . . . . . . . . . . .134 . . . . . . . . . . . . . . . . . . . . . . . . . . . .134 . . . . . . . . . . . . . . . . . . . . . . . . . . . .133 . . . . . . . . . . . . . . . . . . . . . . . . . . . .133 . . . . . . . . . . . . . . . . . . . . . . . . . . . .133 . . . . . . . . . . . . . . . . . . . . . . . .134, 137 . . . . . . . . . . . . . . . . . . . . . . . .134, 137 . . . . . . . . . . . . . . . . . . . . . . . . . . . .134 . . . . . . . . . . . . . . . . . . . . . . . . . . . .134 . . . . . . . . . . . . . . . . . . . . . . . . . . . .134 . . . . . . . . . . . . . . . . . . . . . . . . . . . .134 . . . . . . . . . . . . . . . . . . . . . . . . . . . .114 . . . . . . . . . . . . . . . . . . . . . . . . . . . .116 . . . . . . . . . . . . . . . . . . . . . . . . . . . .134 . . . . . . . . . . . . . . . . . . . . . . . . . . . .134 . . . . . . . . . . . . . . . . . . . . . . . . . . . .134 . . . . . . . . . . . . . . . . . . . . . . . . . . . .119 . . . . . . . . . . . . . . . . . . . . . . . . . . . .136 . . . . . . . . . . . . . . . . . . . . . . . . . . . .137 . . . . . . . . . . . . . . . . . . . . . . . . . . . .136 . . . . . . . . . . . . . . . . . . . . . . . . . . . .137 . . . . . . . . . . . . . . . . . . . . . . . . . . . .136 . . . . . . . . . . . . . . . . . . . . . . . . . . . .137 . . . . . . . . . . . . . . . . . . . . . . . . . . . .137 . . . . . . . . . . . . . . . . . . . . . . . . . . . .137 . . . . . . . . . . . . . . . . . . . . . . . . . . . .137 . . . . . . . . . . . . . . . . . . . . . . . . . . . .137 . . . . . . . . . . . . . . . . . . . . . . . . . . . .136 . . . . . . . . . . . . . . . . . . . . . . . . . . . .137 . . . . . . . . . . . . . . . . . . . . . . . . . . . .137 . . . . . . . . . . . . . . . . . . . . . . . . . . . .137 . . . . . . . . . . . . . . . . . . . . . . . . . . . .134 . . . . . . . . . . . . . . . . . . . . . . . . . . . .134 . . . . . . . . . . . . . . . . . . . . . . . . . . . .134 . . . . . . . . . . . . . . . . . . . . . . . . . . . .134 . . . . . . . . . . . . . . . . . . . . . . . . . . . .134 . . . . . . . . . . . . . . . . . . . . . . . . . . . .118 . . . . . . . . . . . . . . . . . . . . . . . . . . . .135 . . . . . . . . . . . . . . . . . . . . . . . . . . . .137 5185-5799 . . . . . . . . . . . . . . . . . . . . . . . . . . . .137 5185-5800 . . . . . . . . . . . . . . . . . . . . . . . . . . . .212 5185-5807 . . . . . . . . . . . . . . . . . . . . . . . . . . . .596 5185-5808 . . . . . . . . . . . . . . . . . . . . . . . . . . . .596 5185-5809 . . . . . . . . . . . . . . . . . . . . . . . . . . . .596 5185-5810 . . . . . . . . . . . . . . . . . . . . . . . . . . . .596 5185-5811 . . . . . . . . . . . . . . . . . . . . . . . . . . . .121 5185-5812 . . . . . . . . . . . . . . . . . . . . . . . . . . . .121 5185-5813 . . . . . . . . . . . . . . . . . . . . . . . . . . . .121 5185-5814 . . . . . . . . . . . . . . . . . . . . . . . . . . . .121 5185-5815 . . . . . . . . . . . . . . . . . . . . . . . . . . . .121 5185-5816 . . . . . . . . . . . . . . . . . . . . . . . . . . . .129 5185-5817 . . . . . . . . . . . . . . . . . . . . . . . . . . . .100 5185-5821 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .22 5185-5823 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .17 5185-5824 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .17 5185-5830 . . . . . . . . . . . . . . . . . . . . . . . . . . . .147 5185-5831 . . . . . . . . . . . . . . . . . . . . . . . . . . . .147 5185-5832 . . . . . . . . . . . . . . . . . . . . . . . . . . . .147 5185-5833 . . . . . . . . . . . . . . . . . . . . . . . . . . . .147 5185-5834 . . . . . . . . . . . . . . . . . . . . . . . . . . . .147 5185-5835 . . . . . . . . . . . . . . . . . . . . . . . . . . . .147 5185-5836 . . . . . . . . . . . . . . . . . . . . . . . . . . . .146 5185-5837 . . . . . . . . . . . . . . . . . . . . . . . . . . . .146 5185-5838 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .16 5185-5920 . . . . . . . . . . . . . . . . . . . .528, 534, 558 5185-5921 . . . . . . . . . . . . . . . . . . . . . . . . . . . .539 5185-5922 . . . . . . . . . . . . . . . . . . . . . . . . . . . .543 5185-5923 . . . . . . . . . . . . . . . . . . . . . . . .546, 561 550145-118 . . . . . . . . . . . . . . . . . . . . . . . . . . .141 550145-124 . . . . . . . . . . . . . . . . . . . . . . . . . . .141 550145-138 . . . . . . . . . . . . . . . . . . . . . . . . . . .141 550145-148 . . . . . . . . . . . . . . . . . . . . . . . . . . .141 550245-218 . . . . . . . . . . . . . . . . . . . . . . . . . . .141 550245-224 . . . . . . . . . . . . . . . . . . . . . . . . . . .141 550245-238 . . . . . . . . . . . . . . . . . . . . . . . . . . .141 550245-248 . . . . . . . . . . . . . . . . . . . . . . . . . . .141 550550-901 . . . . . . . . . . . . . . . . . . . . . . . . . . .141 590-3003 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .659 590-4000 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .661 590-4001 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .661 590-4005 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .661 5950-3562 . . . . . . . . . . . . . . . . . . . . . . . . . . . .108 5958-9433 . . . . . . . . . . . . . . . . . . . . . . . . . . . .108 5958-9441 . . . . . . . . . . . . . . . . . . . . . . . . . . . .173 5958-9442 . . . . . . . . . . . . . . . . . . . . . . . . . . . .173 5958-9443 . . . . . . . . . . . . . . . . . . . . . . . . . . . .173 5958-9444 . . . . . . . . . . . . . . . . . . . . . . . . . . . .173 5958-9445 . . . . . . . . . . . . . . . . . . . . . . . . . . . .173 5958-9450 . . . . . . . . . . . . . . . . . . . . . . . . . . . .173 5958-9468 . . . . . . . . . . . . . . . . . . . . . . . . . . . .108 5959-3879 . . . . . . . . . . . . . . . . . . . . . . . . . . . .108 5965-0027 . . . . . . . . . . . . . . . . . . . . . . . . .87, 481 5965-0028 . . . . . . . . . . . . . . . . . . . . . . . . .87, 481 5965-0036 . . . . . . . . . . . . . . . . . . . . . . . . . . . .108 5968-6193E . . . . . . . . . . . . . . . . . . . . . . . . . . .109 5968-9963E . . . . . . . . . . . . . . . . . . . . . . . . . . .109 59864B . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .209 59980-20133 . . . . . . . . . . . . . . . . . . . . . . . . . .484 59980-60104 . . . . . . . . . . . . . . . . . . . . . . . . . .484 59987-20040 . . . . . . . . . . . . . . . . . . . . . . . . . .483 59987-60026 . . . . . . . . . . . . . . . . . . . . . . . . . .484 6040-0289 . . . . . . . . . . . . . . . . . . . . . . . . . . . .214 6040-0370 . . . . . . . . . . . . . . . . . . . . . . . . . . . .215 6040-0468 . . . . . . . . . . . . . . . . . . . . . . . . . . . .215 6040-0730 . . . . . . . . . . . . . . . . . . . . . . . .215, 484 6040-0798 . . . . . . . . . . . . . . . .209, 215, 678, 687 6040-0809 . . . . . . . . . . . . . . . . . . . . . . . . . . . .209 6040-0819 . . . . . . . . . . . . . . . . . . . . . . . . . . . .215 6040-0834 . . . . . . . . . . . .209, 215, 483, 678, 687 660750-902 . . . . . . . . . . . . . . . . . . . . . . . . . . .536 677700-902 . . . . . . . . . . . . . . . . . . . . . . . . . . .536 700-0013-50 . . . . . . . . . . . . . . . . . . . . . . . . . .313 700-4005 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .138 700-4006 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .138 700-4007 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .138 700-4008 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .138 700-4015 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .138 700-4016 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .138 700-4030 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .138 700-4031 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .138 701-4000 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .137 701-4005 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .137 705-0825 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .164 705-0903 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .164 705-0905 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .164 705-0925 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .164 707-0027 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .89 715-0006 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .137 735700-902 . . . . . . . . . . . . . . . . . . . . . . .546, 559 735953-902 . . . . . . . . . . . . . . . . . . . . . . .546, 559 735954-302 . . . . . . . . . . . . . . . . . . . . . . . . . . .546 746450-902 . . . . . . . . . . . . . . . . . . . . . . . . . . .546 760450-902 . . . . . . . . . . . . . . . . . . . . . . .546, 559 761600-902 . . . . . . . . . . . . . . . . . . .546, 559, 561 761753-902 . . . . . . . . . . . . . . . . . . . . . . .546, 559 761775-902 . . . . . . . . . . . . . . . . . . . . . . . . . . .549 761973-902 . . . . . . . . . . . . . . . . . . . . . . . . . . .549 763600-902 . . . . . . . . . . . . . . . . . . .546, 559, 561 763750-902 . . . . . . . . . . . . . . . . . . . . . . . . . . .549 763953-902 . . . . . . . . . . . . . . . . . . . . . . .546, 559 763954-302 . . . . . . . . . . . . . . . . . . . . . . . . . . .546 763973-902 . . . . . . . . . . . . . . . . . . . . . . . . . . .549 764953-902 . . . . . . . . . . . . . . . . . . . . . . .546, 559 765600-902 . . . . . . . . . . . . . . . . . . .546, 559, 561 765750-902 . . . . . . . . . . . . . . . . . . . . . . . . . . .549 765973-902 . . . . . . . . . . . . . . . . . . . . . . . . . . .549 766953-902 . . . . . . . . . . . . . . . . . . . . . . .546, 559 770450-902 . . . . . . . . . . . . . . . . . . . . . . . . . . .546 770995-902 . . . . . . . . . . . . . . . . . . . . . . . . . . .549 773450-902 . . . . . . . . . . . . . . . . . . . . . . . . . . .546 773700-902 . . . . . . . . . . . . . . . . . . . . . . .546, 559 773995-902 . . . . . . . . . . . . . . . . . . . . . . . . . . .549 7981618-552 . . . . . . . . . . . . . . . . . . . . . .565, 567 7981618-554 . . . . . . . . . . . . . . . . . . . . . .565, 567 79826-68701 . . . . . . . . . . . . . . . . . . . . . . . . . .496
Indices
737
Part Number Index

79826-68702 . . . . . . . . . . . . . . . . . . . . . . . . . .496 79826-87601 . . . . . . . . . . . . . . . . . . . . . . . . . .496 79826-87602 . . . . . . . . . . . . . . . . . . . . . . . . . .497 79826-87605 . . . . . . . . . . . . . . . . . . . . . . . . . .497 79826-87606 . . . . . . . . . . . . . . . . . . . . . . . . . .497 79826-87607 . . . . . . . . . . . . . . . . . . . . . . . . . .497 79826-87608 . . . . . . . . . . . . . . . . . . . . . .486, 497 7982618-564 . . . . . . . . . . . . . . . . . . . . . .565566 7982618-584 . . . . . . . . . . . . . . . . . . . . . .565566 79826BD-354 . . . . . . . . . . . . . . . . . . . . .565566 79826BD-584 . . . . . . . . . . . . . . . . . . . . .565566 79835-04123 . . . . . . . . . . . . . . . . . . . . . . . . . .494 79835-21734 . . . . . . . . . . . . . . . . . . . . . . . . . .485 79835-25211 . . . . . . . . . . . . . . . . . . . . . . . . . .492 79835-27717 . . . . . . . . . . . . . . . . . . . . . . . . . .494 79835-62701 . . . . . . . . . . . . . . . . . . . . . . . . . .494 79835-65213 . . . . . . . . . . . . . . . . . . . . . . . . . .492 79835-65216 . . . . . . . . . . . . . . . . . . . . . . . . . .492 79835-67101 . . . . . . . . . . . . . . . . . . . . . . . . . .493 79835-67102 . . . . . . . . . . . . . . . . . . . . . . . . . .493 79835-67302 . . . . . . . . . . . . . . . . . . . . . . . . . .494 79835-67304 . . . . . . . . . . . . . . . . . . . . . . . . . .494 79835-67305 . . . . . . . . . . . . . . . . . . . . . . . . . .494 79835-67306 . . . . . . . . . . . . . . . . . . . . . . . . . .494 79835-67307 . . . . . . . . . . . . . . . . . . . . . . . . . .493 79835-67308 . . . . . . . . . . . . . . . . . . . . . . . . . .493 79835-68701 . . . . . . . . . . . . . . . . . . . . . . . . . .494 79835-87330 . . . . . . . . . . . . . . . . . . . . . . . . . .494 79835-87638 . . . . . . . . . . . . . . . . . . . . . .445, 459 79841-65501 . . . . . . . . . . . . . . . . . . . . . . . . . .462 79841-87609 . . . . . . . . . . . . . .445, 514, 518, 598 79846-23203 . . . . . . . . . . . . . . . . . . . . . . . . . .487 79846-24501 . . . . . . . . . . . . . . . . . . . . . .493, 495 79846-24502 . . . . . . . . . . . . . . . . . . . . . .493, 495 79846-27101 . . . . . . . . . . . . . . . . . . . . . .493, 495 79846-27103 . . . . . . . . . . . . . . . . . . . . . .493, 495 79846-27303 . . . . . . . . . . . . . . . . . . . . . . . . . .493 79846-65501 . . . . . . . . . . . . . . . . . . . . . . . . . .463 79846-67101 . . . . . . . . . . . . . . . . . .487, 493, 495 79846-67902 . . . . . . . . . . . . . . . . . . . . . . . . . .493 79846-68701 . . . . . . . . . . . . . . . . . . . . . . . . . .495 79846-68702 . . . . . . . . . . . . . . . . . . . . . . . . . .495 79846-87201 . . . . . . . . . . . . . . . . . . . . . .493, 495 79846-87202 . . . . . . . . . . . . . . . . . . . . . .493, 495 79846-87600 . . . . . . . . . . . . . . . . . . . . . . . . . .493 79846-87601 . . . . . . . . . . . . . . . . . . . . . .493, 495 79846-87604 . . . . . . . . . . . . . . . . . . . . . .493, 495 79846-87605 . . . . . . . . . . . . . . . . . . . . . .493, 495 79846-87609 . . . . . . . . . . . . . . . . . . . . . . . . . .493 79846-87610 . . . . . . . . . . . . . . . . . . . . . . . . . .494 79846-87612 . . . . . . . . . . . . . . . . . . . . . .493, 495 79846-87613 . . . . . . . . . . . . . . . . . . . . . .493, 495 79847-44901 . . . . . . . . . . . . . . . . . . . . . . . . . .495 79847-60005 . . . . . . . . . . . . . . . . . . . . . . . . . .495 79853-22500 . . . . . . . . . . . . . . . . . .475476, 489 79853-27200 . . . . . . . . . . . . .475476, 489490 79853-27201 . . . . . . . . . . . . . . . . . . . . . .489490 79853-27202 . . . . . . . . . . . . . . . . . . . . . .489490 79853-27203 . . . . . . . . . . . . . . . . . . . . . .489490 79853-29100 . . . . . . . . . . . . . . . . . .475476, 489 79853-60000 . . . . . . . . . . . . . . . . . . . . . .456, 489 79853-60002 . . . . . . . . . . . . . . . . . . . . . . . . . .455 79853-60010 . . . . . . . . . . . . . . . . . . . . . .456, 490 79853-60011 . . . . . . . . . . . . . . . . . . . . . .456, 489 79853-60012 . . . . . . . . . . . . . . . . . . . . . .456, 490 79853-60013 . . . . . . . . . . . . . . . . . . . . . .456, 490 79853-68714 . . . . . . . . . . . . . . . . . . . . . . . . . .490 79853-68718 . . . . . . . . . . . . . . . . . . . . . . . . . .489 79853-68719 . . . . . . . . . . . . . . . . . . . . . .489490 79853-68720 . . . . . . . . . . . . . . . . . . . . . .489490 79853-68721 . . . . . . . . . . . . . . . . . . . . . . . . . .489 79853-68722 . . . . . . . . . . . . . . . . . . . . . . . . . .489 79853-68723 . . . . . . . . . . . . . . . . . . . . . . . . . .490 79853-68724 . . . . . . . . . . . . . . . . . . . . . . . . . .490 79853-68726 . . . . . . . . . . . . . . . . . . . . . . . . . .490 79853-68728 . . . . . . . . . . . . . . . . . . . . . . . . . .490 79853-68733 . . . . . . . . . . . . . . . . . . . . . . . . . .490 79853-68734 . . . . . . . . . . . . . . . . . . . . . .476, 490 79853-68738 . . . . . . . . . . . . . . . . . . . . . . . . . .490 79853-68741 . . . . . . . . . . . . . . . . . . . . . . . . . .489 79853-68742 . . . . . . . . . . . . . . . . . .475476, 489 79853-68743 . . . . . . . . . . . . . . . . . .475476, 489 79853-68744 . . . . . . . . . . . . . . . . . . . . . . . . . .489 79853-68745 . . . . . . . . . . . . . . . . . . . . . . . . . .489 79877-68701 . . . . . . . . . . . . . . . . . . . . . . . . . . .94 79880-22711 . . . . . . . . . . . . . . . . . . . . . . . . . .478 79880-60002 . . . . . . . . . . . . . . . . . . . . . .455, 497 79880-67303 . . . . . . . . . . . . . . . . . . . . . . . . . .498 79880-67304 . . . . . . . . . . . . . . . . . . . . . .498499 79881-09103 . . . . . . . . . . . . . . . . . . . . . . . . . .497 79881-27101 . . . . . . . . . . . . . . . . . . . . . . . . . .497 79881-27102 . . . . . . . . . . . . . . . . . . . . . . . . . .497 79881-28101 . . . . . . . . . . . . . . . . . . . . . . . . . .497 79881-60002 . . . . . . . . . . . . . . . . . . . . . . . . . .456 79881-62761 . . . . . . . . . . . . . . . . . . . . . . . . . .497 79881-62763 . . . . . . . . . . . . . . . . . . . . . . . . . .497 79881-62764 . . . . . . . . . . . . . . . . . . . . . . . . . .497 79881-62765 . . . . . . . . . . . . . . . . . . . . . . . . . .497 79881-62769 . . . . . . . . . . . . . . . . . . . . . . . . . .497 79881-67301 . . . . . . . . . . . . . . . . . . . . . .497, 499 79881-67302 . . . . . . . . . . . . . . . . . . . . . .497, 499 79881-68701 . . . . . . . . . . . . . . . . . . . . . . . . . .497 79883-22301 . . . . . . . . . . . . . .478479, 491, 498 79883-22402 . . . . . . . . . . . . . .478479, 491, 498 79883-22404 . . . . . . . . . . . . . . . . . . . . . . . . . .479 79883-27101 . . . . . . . . . . . . . . . . . . . . . .479, 491 79883-28801 . . . . . . . . . . . . . .478479, 491, 498 79883-28802 . . . . . . . . . . . . . . . . . . . . . . . . . .479 79883-60002 . . . . . . . . . . . . . .455, 489, 491, 498 79883-60041 . . . . . . . . . . . . . . . . . . . . . . . . . .499 79883-60042 . . . . . . . . . . . . . . . . . . . . . . . . . .499 79883-60051 . . . . . . . . . . . . . . . . . . . . . .456, 491 79883-60052 . . . . . . . . . . . . . . . . . . . . . .456, 491 79883-60054 . . . . . . . . . . . . . . . . . . . . . .456, 491 79883-60055 . . . . . . . . . . . . . . . . . .456, 491, 498 79883-60091 . . . . . . . . . . . . . . . . . . . . . .456, 498 79883-60092 . . . . . . . . . . . . . . . . . . . . . .456, 498 79883-68700 . . . . . . . . . . . . . . . . . .479, 491, 498 79883-68701 . . . . . . . . . . . . . . . . . . . . . .491, 498 79883-68702 . . . . . . . . . . . . . . . . . .479, 491, 498 79883-68703 . . . . . . . . . . . . . . . . . .478479, 491 79883-80002 . . . . . . . . . . . . . . . . . . . . . . . . . .491 79883-80004 . . . . . . . . . . . . . . . . . . . . . . . . . .491 79883-80008 . . . . . . . . . . . . . . . . . . . . . . . . . .491 79883-80022 . . . . . . . . . . . . . . . . . . . . . . . . . .498 79883-80024 . . . . . . . . . . . . . . . . . . . . . . . . . .498 79883-80028 . . . . . . . . . . . . . . . . . . . . . . . . . .498 79883-87303 . . . . . . . . . . . . . . . . . . . . . . . . . .491 79883-87304 . . . . . . . . . . . . . . . . . . . . . . . . . .491 79883-87305 . . . . . . . . . . . . . . . . . . . . . .491, 498 79883-87306 . . . . . . . . . . . . . . . . . . . . . .491, 498 79883-87322 . . . . . . . . . . . . . . . . . . . . . .498499 79883-87328 . . . . . . . . . . . . . . . . . . . . . . . . . .499 79900CH-010 . . . . . . . . . . . . .514, 567, 575, 598 79903-85021 . . . . . . . . . . . . . . . . . . . . . . . . . .518 79903-85031 . . . . . . . . . . . . . . . . . . . . . . . . . .518 79903-85037 . . . . . . . . . . . . . . . . . . . . . . . . . .570 79911-60500 . . . . . . . . . . . . . . . . . . . . . . . . . . .92 79911-60501 . . . . . . . . . . . . . . . . . . . . . . . . . . .92 79911-60502 . . . . . . . . . . . . . . . . . . . . . . . . . . .92 79911GF-080 . . . . . . . . . . . . . . . . . . . . . . . . . .588 79911GF-083 . . . . . . . . . . . . . . . . . . . . . . . . . .588 79911GF-084 . . . . . . . . . . . . . . . . . . . . . . . . . .588 79911GF-085 . . . . . . . . . . . . . . . . . . . . . . . . . .588 79911GF-086 . . . . . . . . . . . . . . . . . . . . . . . . . .588 79911GF-MXA . . . . . . . . . . . . . . . . . . . . . . . .588 79911GP-100 . . . . . . . . . . . . . . . . . . . . . . . . .584 79911GP-101 . . . . . . . . . . . . . . . . . . . . . . . . .584 79911GP-102 . . . . . . . . . . . . . . . . . . . . . . . . .584 79911GP-103 . . . . . . . . . . . . . . . . . . . . . . . . .584 79911GP-104 . . . . . . . . . . . . . . . . . . . . . . . . .584 79911GP-105 . . . . . . . . . . . . . . . . . . . . . . . . .584 79911GP-106 . . . . . . . . . . . . . . . . . . . . . . . . .584 79911GP-110 . . . . . . . . . . . . . . . . . . . . . . . . .584 79911GP-500 . . . . . . . . . . . . . . . . . . . . . . . . .584 79911GP-501 . . . . . . . . . . . . . . . . . . . . . . . . .584 79911GP-502 . . . . . . . . . . . . . . . . . . . . . . . . .584 79911GP-503 . . . . . . . . . . . . . . . . . . . . . . . . .584 79911GP-504 . . . . . . . . . . . . . . . . . . . . . . . . .584 79911GP-505 . . . . . . . . . . . . . . . . . . . . . . . . .584 79911GP-510 . . . . . . . . . . . . . . . . . . . . . . . . .584 79911GP-MXA . . . . . . . . . . . . . . . . . . . . . . . .584 79911GP-MXB . . . . . . . . . . . . . . . . . . . . . . . .584 79911GP-MXC . . . . . . . . . . . . . . . . . . . . . . . .584 79911GP-MXD . . . . . . . . . . . . . . . . . . . . . . . .584 79911GP-MXE . . . . . . . . . . . . . . . . . . . . . . . .584 79915MO-174 . . . . . . . . . . . . . . . . . . . . . . . . .569 79915MO-574 . . . . . . . . . . . . . . . . . . . . . . . . .569 7991618-584 . . . . . . . . . . . . . . . . . . . . . . . . . .567 79916AA-572 . . . . . . . . . . . . . . . . . . . . . . . . .598 79916AP-552 . . . . . . . . . . . . . . . . . . . . . . . . .575 79916AP-554 . . . . . . . . . . . . . . . . . . . . . . . . .575 79916AP-574 . . . . . . . . . . . . . . . . . . . . . . . . .575 79916BD-354 . . . . . . . . . . . . . . . . . . . . . . . . .567
Indices
738
Part Number Index

79916BD-584 . . . . . . . . . . . . . . . . . . . . . . . . .567 79916KT-100 . . . . . . . . . . . . . . . . . . . . . . . . . .518 79916KT-101 . . . . . . . . . . . . . . . . . . . . . . . . . .518 79916KT-102 . . . . . . . . . . . . . . . . . . . . . . . . . .518 79916KT-110 . . . . . . . . . . . . . . . . . . . . . .567, 598 79916KT-111 . . . . . . . . . . . . . . . . . . . . . . . . . .567 79916KT-112 . . . . . . . . . . . . . . . . . . . . . . . . . .575 79916KT-113 . . . . . . . . . . . . . . . . . . . . . . . . . .575 79916KT-120 . . . . . . . . . . . . . . . . . . . . . . . . . .567 79916KT-121 . . . . . . . . . . . . . . . . . . . . . . . . . .567 79916KT-122 . . . . . . . . . . . . . . . . . . . . . . . . . .575 79916KT-123 . . . . . . . . . . . . . . . . . . . . . . . . . .575 79916MO-552 . . . . . . . . . . . . . . . . . . . . . . . . .567 79916MO-554 . . . . . . . . . . . . . . . . . . . . . . . . .567 79916MO-572 . . . . . . . . . . . . . . . . . . . . . . . . .567 79916MO-574 . . . . . . . . . . . . . . . . . . . . . . . . .567 79916OD-344 . . . . . . . . . . . . . . . . . . . . . . . . .567 79916OD-352 . . . . . . . . . . . . . . . . . . . . .567, 599 79916OD-552 . . . . . . . . . . . . . . . . . . . . . . . . .567 79916OD-554 . . . . . . . . . . . . . . . . . . . . . . . . .567 79916OD-572 . . . . . . . . . . . . . . . . . . . . . . . . .567 79916OD-574 . . . . . . . . . . . . . . . . . . . . . . . . .567 79916PT-582 . . . . . . . . . . . . . . . . . . . . . .567, 599 79916SI-552 . . . . . . . . . . . . . . . . . . . . . . . . . .575 79916SI-554 . . . . . . . . . . . . . . . . . . . . . . . . . .575 79916SI-572 . . . . . . . . . . . . . . . . . . . . . . . . . .575 79916SI-574 . . . . . . . . . . . . . . . . . . . . . . . . . .575 79918O3-564 . . . . . . . . . . . . . . . . . . . . . . . . .572 79918O3-581 . . . . . . . . . . . . . . . . . . . . . . . . .572 79918O3-582 . . . . . . . . . . . . . . . . . . . . . . . . .572 79918O3-584 . . . . . . . . . . . . . . . . . . . . . . . . .572 79918O3-588 . . . . . . . . . . . . . . . . . . . . . . . . .572 79918PAH-582 . . . . . . . . . . . . . . . . . . . . . . . .599 79918PAH-584 . . . . . . . . . . . . . . . . . . . . . . . .599 79918PH-100 . . . . . . . . . . . . . . . . . . . . . . . . .599 79918PH-532 . . . . . . . . . . . . . . . . . . . . . . . . .599 79918PH-534 . . . . . . . . . . . . . . . . . . . . . . . . .599 79919O3-554 . . . . . . . . . . . . . . . . . . . . . . . . .572 79919O3-584 . . . . . . . . . . . . . . . . . . . . . . . . .572 79919P3-754 . . . . . . . . . . . . . . . . . . . . . . . . . .591 79919QA-754 . . . . . . . . . . . . . . . . . . . . . . . . .591 79919QA-784 . . . . . . . . . . . . . . . . . . . . . . . . .591 79919SP-754 . . . . . . . . . . . . . . . . . . . . . . . . . .591 79919SP-784 . . . . . . . . . . . . . . . . . . . . . . . . . .591 7992318-562 . . . . . . . . . . . . . . . . . . . . . . . . . .570 79923OP-564 . . . . . . . . . . . . . . . . . . . . .570, 599 79923OP-584 . . . . . . . . . . . . . . . . . . . . . . . . .570 7992518-402 . . . . . . . . . . . . . . . . . . . . . . . . . .571 7992518-484 . . . . . . . . . . . . . . . . . . . . . . . . . .571 79925AP-504 . . . . . . . . . . . . . . . . . . . . . . . . .575 79925AP-564 . . . . . . . . . . . . . . . . . . . . . . . . .575 79925AP-584 . . . . . . . . . . . . . . . . . . . . . . . . .575 79925CB-584 . . . . . . . . . . . . . . . . . . . . . . . . .577 79925CN-504 . . . . . . . . . . . . . . . . . . . . . . . . .568 79925CN-564 . . . . . . . . . . . . . . . . . . . . . . . . .568 79925CN-584 . . . . . . . . . . . . . . . . . . . . . . . . .568 79925EC-462 . . . . . . . . . . . . . . . . . . . . . . . . . .571 79925EC-482 . . . . . . . . . . . . . . . . . . . . . . . . . .571 79925OD-464 . . . . . . . . . . . . . . . . . . . . . . . . .571 79925SPE-504 . . . . . . . . . . . . . . . . . . . . . . . .570 79925PA-583 . . . . . . . . . . . . . . . . . . . . . . . . .599 79925PE-564 . . . . . . . . . . . . . . . . . . . . . . . . . .570 79925PE-584 . . . . . . . . . . . . . . . . . . . . . . . . . .570 79925PU-504 . . . . . . . . . . . . . . . . . . . . . . . . .570 79925PU-564 . . . . . . . . . . . . . . . . . . . . . . . . .570 79925PU-584 . . . . . . . . . . . . . . . . . . . . . . . . .570 79925SB-563 . . . . . . . . . . . . . . . . . . . . . . . . .568 79925SB-56K . . . . . . . . . . . . . . . . . . . . . . . . .568 79925SB-583 . . . . . . . . . . . . . . . . . . . . . . . . .568 79925SB-58K . . . . . . . . . . . . . . . . . . . . . . . . .568 7992618-362 . . . . . . . . . . . . . . . . . . . . . . . . . .566 7992618-363 . . . . . . . . . . . . . . . . . . . . . . . . . .566 7992618-504 . . . . . . . . . . . . . . . . . . . . . . . . . .566 7992618-562 . . . . . . . . . . . . . . . . . . . . . . . . . .566 7992618-582 . . . . . . . . . . . . . . . . . . . . . . . . . .566 7992618-585 . . . . . . . . . . . . . . . . . . . . . . . . . .566 7992618-595 . . . . . . . . . . . . . . . . . . . . . . . . . .566 79926B8-344 . . . . . . . . . . . . . . . . . . . . . . . . .566 79926B8-562 . . . . . . . . . . . . . . . . . . . . . . . . .566 79926B8-564 . . . . . . . . . . . . . . . . . . . . . . . . .566 79926B8-582 . . . . . . . . . . . . . . . . . . . . . . . . .566 79926B8-584 . . . . . . . . . . . . . . . . . . . . . . . . .566 79926BD-344 . . . . . . . . . . . . . . . . . . . . . . . . .566 79926BD-362 . . . . . . . . . . . . . . . . . . . . . . . . .566 79926BD-363 . . . . . . . . . . . . . . . . . . . . . . . . .566 79926BD-504 . . . . . . . . . . . . . . . . . . . . . . . . .566 79926BD-562 . . . . . . . . . . . . . . . . . . . . . . . . .566 79926BD-582 . . . . . . . . . . . . . . . . . . . . . . . . .566 79926BD-585 . . . . . . . . . . . . . . . . . . . . . . . . .566 79926BD-595 . . . . . . . . . . . . . . . . . . . . . . . . .566 79926KT-100 . . . . . . . . . . . . . . . . . . . . . .566, 597 79926OB-354 . . . . . . . . . . . . . . . . . . . . . . . . .566 79926OB-564 . . . . . . . . . . . . . . . . . . . . . . . . .566 79926OB-584 . . . . . . . . . . . . . . . . . . . . . . . . .566 79926OD-354 . . . . . . . . . . . . . . . . . . . . . . . . .566 79926OD-564 . . . . . . . . . . . . . . . . . . . . . . . . .566 79926OD-584 . . . . . . . . . . . . . . . . . . . . . . . . .566 7992718-504 . . . . . . . . . . . . . . . . . . . . . . . . . .569 7992718-564 . . . . . . . . . . . . . . . . . . . . . . . . . .569 7992718-584 . . . . . . . . . . . . . . . . . . . . . . . . . .569 79931QA-835 . . . . . . . . . . . . . . . . . . . . . . . . .591 7995108-344 . . . . . . . . . . . . . . . . . . . . . . . . . .540 7995108-585 . . . . . . . . . . . . . . . . . . . . . . . . . .540 7995108-595 . . . . . . . . . . . . . . . . . . . . . . . . . .540 7995118-504 . . . . . . . . . . . . . . . . . . . . . .529, 540 7995208-344 . . . . . . . . . . . . . . . . . . . . . . . . . .529 7995208-585 . . . . . . . . . . . . . . . . . . . . . . . . . .529 7995208-595 . . . . . . . . . . . . . . . . . . . . . . . . . .529 7995230-344 . . . . . . . . . . . . . . . . . . . . . . . . . .540 820222-901 . . . . . . . . . . . . . . .516, 529, 540, 557 820311-001 . . . . . . . . . . . . . . . . . . . . . . . . . . .516 820315-015 . . . . . . . . . . . . . . . . . . . . . . . . . . .516 820330-030 . . . . . . . . . . . . . . . . . . . . . . . . . . .516 820333-901 . . . . . . . . . . . . . . . . . . . . . . . . . . .536 820370-901 . . . . . . . . . . . . . . .516, 529, 540, 557 820385-901 . . . . . . . . . . . . . . . . . . .529, 540, 553 820400-901 . . . . . . . . . . . . . . . . . . .529, 540, 553 820563-901 . . . . . . . . . . . . . . . . . . . . . . . . . . .515 820565-001 . . . . . . . . . . . . . . . . . . . . . . . . . . .515 820572-001 . . . . . . . . . . . . . . . . . . . . . . . . . . .515 820675-111 . . . . . . . . . . . . . . . . . . . . . . .574, 585 820675-115 . . . . . . . . . . . . . . .528, 551, 553, 558 820675-124 . . . . . . . . . . . . . . . . . . . . . . .534, 562 820777-901 . . . . 515, 528, 531, 534, 539, 543, 546, 549, 551, 558, 562, 574, 585, 590, 592593, 598 820940-901 . . . . . . . . . . . . . . . . . . . . . . . . . . .594 820950-904 . . . . . . . . . . . . . . . . . . . . . . . . . . .590 820950-905 . . . . . . . . . . . . . . . . . . . . . . . . . . .562 820950-908 . . . . . . . . . . . . . . . . . . . . . . .574, 592 820950-911 . . . . . . . . . . . . . . . . . . . . . . . . . . .585 820950-913 . . . . . . . . . . . . . . . . . . . . . . . . . . .551 820950-921 . . . . . . . . . . . . . . . . . . . . . . . . . . .534 820950-927 . . . . . . . . . . . . . . . . . . . . . . . . . . .539 820950-928 . . . . . . . . . . . . . . . . . . . . . . . . . . .543 820950-929 . . . . . . . . . . . . . . . . . . . . . . . . . . .593 820950-930 . . . . . . . . . . . . . . . . . . . . . . . . . . .546 820950-931 . . . . . . . . . . . . . . . . . . . . . . . . . . .598 820950-932 . . . . . . . . . . . . . . . . . . . . . . . . . . .549 820950-933 . . . . . . . . . . . . . . . . . . .528, 531, 558 821075-920 . . . . . . . . . . . . . . . . . . . . . . . . . . .536 821125-918 . . . . . . . . . . . . . . . . . . . . . . . . . . .534 821125-919 . . . . . . . . . . . . . . . . . . . . . . . . . . .574 821125-926 . . . . . . . . . . . . . . . . . . . . . . .539, 551 821125-928 . . . . . . . . . . . . . . . . . . . . . . . . . . .543 821125-929 . . . . . . . . . . . . . . . . . . . . . . . . . . .593 821125-930 . . . . . . . . . . . . . . . . . . . . . . . . . . .546 821125-932 . . . . . . . . . . . . . . . . . . . . . . . . . . .549 821125-933 . . . . . . . . . . . . . . . . . . .528, 531, 558 82167-60003 . . . . . . . . . . . . . . . . . . . . . . . . . .106 830990-914 . . . . . . . . . . . . . . . . . . .528, 531, 558 831975-906 . . . . . . . . . . . . . . . . . . . . . . . . . . .529 831975-936 . . . . . . . . . . . . . . . . . . . . . . . . . . .529 833975-906 . . . . . . . . . . . . . . . . . . . . . . . . . . .529 833975-936 . . . . . . . . . . . . . . . . . . . . . . . . . . .529 835975-914 . . . . . . . . . . . . . . . . . . .528, 531, 558 840140-901 . . . . .517, 528, 534, 551, 553, 558, 562, 574, 585 840300-908 . . . . . . . . . . . . . . . . . . . . . . .574, 592 843300-908 . . . . . . . . . . . . . . . . . . . . . . .574, 592 846952-704 . . . . . . . . . . . . . . . . . . . . . . . . . . .590 846975-202 . . . . . . . . . . . . . . . . . . . . . . .528, 553 846975-914 . . . . . . . . . . . . . . . . . . . . . . .528, 531 8500-0010 . . . . . . . . . . . . . . . . . . . . . . . . . . . .174 8500-0656 . . . . . . . . . . . . . . . .205, 208, 214, 216 8500-1233 . . . . . . . . . . . . . . . . . . . . . . . . . . . .205 8500-4328 . . . . . . . . . . . . . . . . . . . . . . . . .98, 174 8500-4410 . . . . . . . . . . . . . . . . . . . . . . . . . . . .657 8500-4789 . . . . . . . . . . . . . . . . . . . . . . . . .98, 188 8500-5067 . . . . . . . . . . . . . . . . . . . . . . . . .98, 197 8500-5275 . . . . . . . . . . . . . . . . . . . . . . . . . . . .484 8500-5440 . . . . . . . . . . . . . . . . . . . . . . . . . . . .216 8500-5441 . . . . . . . . . . . . . . . . . . . . . . . . . . . .216 8500-5500 . . . . . . . . . . . . . . . . . . . . . . . . . . . .216
Indices
739
Part Number Index

8500-5808 . . . . . . . . . . . . . . . . . . . . . . . . . . . .216 8500-5851 . . . . . . . . . . . . . . . . . . . . . . . . . . . .216 8500-5901 . . . . . . . . . . . . . . . . . . . . . . . . . . . .172 8500-5995 . . . . . . . . . . . . . . . . . . . . . . . . . . . .216 8500-6035 . . . . . . . . . . . . . . . . . . . . . . . . . . . .599 8500-6405 . . . . . . . . . . . . . . . . . . . . . . . . . . . .216 8500-6406 . . . . . . . . . . . . . . . . . . . . . . . . . . . .216 8500-6572 . . . . . . . . . . . . . . . . . . . . . . . . . . . .216 8500-6762 . . . . . . . . . . . . . . . . . . . . . . . . .94, 482 8500-6782 . . . . . . . . . . . . . . . . . . . . . . . . . . . .654 8500-6785 . . . . . . . . . . . . . . . . . . . . . . . . . . . .667 8500-6786 . . . . . . . . . . . . . . . . . . . . . . . . . . . .654 8500-6787 . . . . . . . . . . . . . . . . . . . . . . . . . . . .654 8500-6797 . . . . . . . . . . . . . . . . . . . . . . . . . . . .666 8500-6812 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .97 8500-6813 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .97 8500-6900 . . . . . . . . . . . . . . . . . . . . . . . . . . . .667 8500-6917 . . . . . . . . . . . . . . . . . . . . .94, 482483 8500-6936 . . . . . . . . . . . . . . . . . . . . . . . . . . . .689 8500-6937 . . . . . . . . . . . . . . . . . . . . . . . . . . . .689 8500-6938 . . . . . . . . . . . . . . . . . . . . . . . . . . . .689 8500-6939 . . . . . . . . . . . . . . . . . . . . . . . . . . . .689 8500-6940 . . . . . . . . . . . . . . . . . . . . . . . . . . . .689 8500-6941 . . . . . . . . . . . . . . . . . . . . . . . . . . . .689 8500-6942 . . . . . . . . . . . . . . . . . . . . . . . . . . . .689 8500-6943 . . . . . . . . . . . . . . . . . . . . . . . . . . . .689 8500-6944 . . . . . . . . . . . . . . . . . . . . . . . . . . . .689 8500-6945 . . . . . . . . . . . . . . . . . . . . . . . . . . . .689 8500-6946 . . . . . . . . . . . . . . . . . . . . . . . . . . . .689 8500-6947 . . . . . . . . . . . . . . . . . . . . . . . . . . . .689 8500-6948 . . . . . . . . . . . . . . . . . . . . . . . . . . . .689 8500-6998 . . . . . . . . . . . . . . . . . . . . . . . . . . . .690 8500-7000 . . . . . . . . . . . . . . . . . . . . . . . . . . . .689 8500-7001 . . . . . . . . . . . . . . . . . . . . . . . . . . . .690 8500-8130 . . . . . . . . . . . . . . . . . . . . . . . .208, 216 860700-304 . . . . . . . . . . . . . . . . . . . . . . . . . . .590 860700-704 . . . . . . . . . . . . . . . . . . . . . . . . . . .590 860950-902 . . . . . . . . . . . . . . . . . . . . . . . . . . .534 860975-914 . . . . . . . . . . . . . . . . . . .528, 531, 558 861600-902 . . . . . . . . . . . . . . . . . . . . . . . . . . .528 861600-906 . . . . . . . . . . . . . . . . . . . . . . . . . . .558 861608-901 . . . . . . . . . . . . . . . . . . . . . . .543, 559 861700-901 . . . . . . . . . . . . . . . . . . . . . . .543, 559 861753-902 . . . . . . . . . . . . . . . . . . . . . . .558, 559 861753-905 . . . . . . . . . . . . . . . . . . . . . . . . . . .558 861753-906 . . . . . . . . . . . . . . . . . . .551, 558, 559 861753-912 . . . . . . . . . . . . . . . . . . . . . . . . . . .558 861753-914 . . . . . . . . . . . . . . . . . . .528, 531, 558 861768-901 . . . . . . . . . . . . . . . . . . . . . . .543, 559 861775-902 . . . . . . . . . . . . . . . . . . . . . . . . . . .534 861953-906 . . . . . . . . . . . . . . . . . . . . . . . . . . .551 861953-914 . . . . . . . . . . . . . . . . . . .528, 531, 558 861954-306 . . . . . . . . . . . . . . . . . . . . . . .528, 558 861971-901 . . . . . . . . . . . . . . . . . . . . . . .543, 559 861973-306 . . . . . . . . . . . . . . . . . . . . . . . . . . .534 861973-902 . . . . . . . . . . . . . . . . . . . . . . . . . . .534 863600-902 . . . . . . . . . . . . . . . . . . . . . . . . . . .528 863600-905 . . . . . . . . . . . . . . . . . . . . . . . . . . .528 863600-906 . . . . . . . . . . . . . . . . . . . . . . . . . . .558 863608-901 . . . . . . . . . . . . . . . . . . . . . . .543, 559 863630-902 . . . . . . . . . . . . . . . . . . . . . . . . . . .534 863668-901 . . . . . . . . . . . . . . . . . . . . . . .543, 559 863953-906 . . . . . . . . . . . . . . . . . . . . . . . . . . .551 863953-914 . . . . . . . . . . . . . . . . . . .528, 531, 558 863954-312 . . . . . . . . . . . . . . . . . . . . . . .528, 558 863973-902 . . . . . . . . . . . . . . . . . . . . . . . . . . .534 863974-306 . . . . . . . . . . . . . . . . . . . . . . . . . . .534 864668-901 . . . . . . . . . . . . . . . . . . . . . . .543, 559 8650-0029 . . . . . . . . . . . . . . . . . . . . . . . .202, 205 8650-0030 . . . . . . . . . . . . . . . . . . . .202, 205, 214 865600-902 . . . . . . . . . . . . . . . . . . . . . . . . . . .528 865600-906 . . . . . . . . . . . . . . . . . . . . . . . . . . .558 865608-901 . . . . . . . . . . . . . . . . . . . . . . .543, 559 865630-902 . . . . . . . . . . . . . . . . . . . . . . . . . . .534 865750-902 . . . . . . . . . . . . . . . . . . . . . . . . . . .534 865973-902 . . . . . . . . . . . . . . . . . . . . . . . . . . .534 8660-0791 . . . . . . . . . . . . . . . . . . . . . . . .205, 214 8660-0852 . . . . . . . . . . . . . . . . . . . . . . . . . . . .483 866668-901 . . . . . . . . . . . . . . . . . . . . . . .543, 559 866735-902 . . . . . . . . . . . . . . . . . . . . . . . . . . .528 866953-906 . . . . . . . . . . . . . . . . . . . . . . . . . . .551 866953-914 . . . . . . . . . . . . . . . . . . .528, 531, 558 870050-906 . . . . . . . . . . . . . . . . . . . . . . . . . . .529 870100-906 . . . . . . . . . . . . . . . . . . . . . . . . . . .529 870150-906 . . . . . . . . . . . . . . . . . . . . . . . . . . .529 8710-0004 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .87 8710-0007 . . . . . . . . . . . . . . . . . . . . . . . . .87, 497 8710-0018 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .87 8710-0510 . . . .7172, 86, 163, 189, 205, 481, 492 8710-0641 . . . . . . . . . . . . . . . . . . . . .87, 481, 492 8710-0803 . . . . . . . . . . . . . . . . . . . . . . .7172, 86 8710-0806 . . . . . . . . . . . . . . . . . . .7172, 86, 492 8710-0899 . . . . . . . . . . . . . . . . . .72, 87, 205, 492 8710-0900 . . . . . . . . . . . . . . . . . .72, 87, 205, 492 8710-0972 . . . . . . . . . . . . . . . . . . . . . . .7172, 86 8710-0979 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .38 8710-1158 . . . . . . . . . . . . . . . . . . . . . . . . . . . .494 8710-1217 . . . . . . . . . . . . . . . . . . . . . . . . . .7172 8710-1220 . . . . . . . . . . . . . . . . . . . . . . . . . . . .205 8710-1346 . . . . . . . . . . . . . . . . . . . . . . . .182, 193 8710-1561 . . . . . . . . . . . . . . . . . . . . . . . .182, 193 8710-1615 . . . . . . . . . . . . . . . . . . . . . . . . . . . .205 8710-1622 . . . . . . . . . . . . . . . . . . . . . . . . . . . .205 8710-1643 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .38 8710-1707 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .87 8710-1708 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .87 8710-1709 . . . . . . . . . . . . . . . . . . . . . . .7172, 87 8710-1710 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .87 8710-1712 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .86 8710-1876 . . . . . . . . . . . . . . . . . . . . . . . . . . . .197 8710-1924 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .86 8710-1930 . . . . . . . . . . . . . . . . . . . . . . . . . . . .447 8710-1931 . . . . . . . . . . . . . . . . . . . . . . . . . . . .447 8710-1938 . . . . . . . . . . . . . . . . . . . . . . . . . . . .197 8710-2023 . . . . . . . . . . . . . . . . . . . . . . . . . . . .491 8710-2392 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .87 8710-2393 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .87 8710-2394 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .87 8710-2411 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .87 8710-2412 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .87 871700-914 . . . . . . . . . . . . . . . . . . .528, 531, 558 8720-0010 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .86 8720-0025 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .86 873700-906 . . . . . . . . . . . . . . . . . . . . . . . . . . .529 873700-936 . . . . . . . . . . . . . . . . . . . . . . . . . . .529 875700-906 . . . . . . . . . . . . . . . . . . . . . . . . . . .529 875700-936 . . . . . . . . . . . . . . . . . . . . . . . . . . .529 880668-901 . . . . . . . . . . . . . . . . . . . . . . . . . . .543 880949-903 . . . . . . . . . . . . . . . . . . . . . . . . . . .581 880949-904 . . . . . . . . . . . . . . . . . . . . . . . . . . .581 880952-105 . . . . . . . . . . . . . . . . . . . . . . . . . . .562 880952-108 . . . . . . . . . . . . . . . . . . . . . . . . . . .574 880952-204 . . . . . . . . . . . . . . . . . . . . . . . . . . .590 880952-205 . . . . . . . . . . . . . . . . . . . . . . . . . . .562 880952-208 . . . . . . . . . . . . . . . . . . . . . . . . . . .574 880952-302 . . . . . . . . . . . . . . . . . . . . . . . . . . .562 880952-701 . . . . . . . . . . . . . . . . . . . . . . . . . . .574 880952-704 . . . . . . . . . . . . . . . . . . . . . . . . . . .590 880952-710 . . . . . . . . . . . . . . . . . . . . . . . . . . .562 880957-801 . . . . . . . . . . . . . . . . . . . . . . . . . . .581 880957-802 . . . . . . . . . . . . . . . . . . . . . . . . . . .581 880957-805 . . . . . . . . . . . . . . . . . . . . . . . . . . .581 880957-806 . . . . . . . . . . . . . . . . . . . . . . . . . . .581 880957-807 . . . . . . . . . . . . . . . . . . . . . . . . . . .581 880957-808 . . . . . . . . . . . . . . . . . . . . . . . . . . .581 880957-809 . . . . . . . . . . . . . . . . . . . . . . . . . . .581 880957-814 . . . . . . . . . . . . . . . . . . . . . . . . . . .581 880967-106 . . . . . . . . . . . . . . . . . . . . . . . . . . .551 880967-201 . . . . . . . . . . . . . . . . . . . . . . . . . . .551 880967-901 . . . . . . . . . . . . . . . . . . . . . . . . . . .551 880975-112 . . . . . . . . . . . . . . . . . . . . . . . . . . .528 880975-212 . . . . . . . . . . . . . . . . . . . . . . . . . . .528 880975-306 . . . . . . . . . . . . . . . . . . . . . . . . . . .551 880975-312 . . . . . . . . . . . . . . . . . . . . . . . . . . .528 880975-914 . . . . . . . . . . . . . . . . . . . . . . .528, 531 880995-109 . . . . . . . . . . . . . . . . . . . . . . . . . . .534 880995-202 . . . . . . . . . . . . . . . . . . . . . . . . . . .534 880995-902 . . . . . . . . . . . . . . . . . . . . . . . . . . .534 883668-901 . . . . . . . . . . . . . . . . . . . . . . . . . . .543 883700-704 . . . . . . . . . . . . . . . . . . . . . . . . . . .590 883700-901 . . . . . . . . . . . . . . . . . . . . . . . . . . .574 883700-906 . . . . . . . . . . . . . . . . . . . . . . . . . . .551 883700-912 . . . . . . . . . . . . . . . . . . . . . . .528, 558 883725-901 . . . . . . . . . . . . . . . . . . . . . . .543, 559 883750-902 . . . . . . . . . . . . . . . . . . . . . . . . . . .534 883952-302 . . . . . . . . . . . . . . . . . . . . . . . . . . .562 883952-701 . . . . . . . . . . . . . . . . . . . . . . . . . . .574 883952-704 . . . . . . . . . . . . . . . . . . . . . . . . . . .590 883952-710 . . . . . . . . . . . . . . . . . . . . . . . . . . .562 883967-901 . . . . . . . . . . . . . . . . . . . . . . . . . . .551 883975-202 . . . . . . . . . . . . . . . . . . . . . . . . . . .528 883975-306 . . . . . . . . . . . . . . . . . . . . . . . . . . .551 883975-312 . . . . . . . . . . . . . . . . . . . . . . . . . . .528 883975-902 . . . . . . . . . . . . . . . . . . . . . . . . . . .553
Indices
740
Part Number Index

883975-914 . . . . . . . . . . . . . . . . . . . . . . .528, 531 883995-902 . . . . . . . . . . . . . . . . . . . . . . . . . . .534 884950-543 . . . . . . . . . . . . . . . . . . . . . . . . . . .562 884973-701 . . . . . . . . . . . . . . . . . . . . . . . . . . .585 884973-901 . . . . . . . . . . . . . . . . . . . . . . . . . . .585 884973-902 . . . . . . . . . . . . . . . . . . . . . . . . . . .585 884973-903 . . . . . . . . . . . . . . . . . . . . . . . . . . .594 884974-901 . . . . . . . . . . . . . . . . . . . . . . . . . . .585 884974-910 . . . . . . . . . . . . . . . . . . . . . . . . . . .585 884974-915 . . . . . . . . . . . . . . . . . . . . . . . . . . .594 884975-202 . . . . . . . . . . . . . . . . . . . . . . .528, 553 89075-23800 . . . . . . . . . . . . . . . . . . . . . . .94, 696 89075-60002 . . . . . . . . . . . . . . . . . . . . . . . . . .696 900-1010 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .108 900-1013 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .108 907-0056 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .77 9270-0658 . . . . . . . . . . . . . . . . . . . . . . . . . . . .103 9300-0311 . . . . . . . . . . . . . . . . . . . .7172, 79, 90 9300-0761 . . . . . . . . . . . . . . . . . . . . . . . . . . . .694 9300-1747 . . . . . . . . . . . . . . . . . . . . . . . . . . . .656 9300-1748 . . . . . . . . . . . . . . . . . . . . . . . . . . . .656 9301-0511 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .72 9301-0633 . . . . . . . . . . . . . . . . . . . . . . . .493, 495 9301-0656 . . . . . . . . . . . . . . . . . . . . . . . .485, 492 9301-0658 . . . . . . . . . . . . . . . . . . . . .45, 178, 190 9301-0675 . . . . . . . . . . . . . . . . . . . . . . . . . . . .495 9301-0677 . . . . . . . . . . . . . . . . . . . . . . . . . . . .495 9301-0678 . . . . . . . . . . . . . . . . . . . . . . . .493, 495 9301-0713 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .41 9301-0714 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .41 9301-0718 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .34 9301-0719 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .35 9301-0720 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .38 9301-0721 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .34 9301-0722 . . . . . . . . . . . . . . . . . . . . . . . . .38, 656 9301-0723 . . . . . . . . . . . . . . . . . . . . . . . . .32, 173 9301-0725 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .41 9301-0891 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .41 9301-0892 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .41 9301-0895 . . . . . . . . . . . . . . . . . . . . . . . . . . . .449 9301-0976 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .35 9301-0977 . . . . . . . . . . . . . . . . . . . . . .22, 26, 654 9301-0978 . . . . . . . . . . . . . . . . . . . . . .22, 25, 654 9301-0985 . . . . . . . . . . . . . . . . . . . . .90, 182, 192 9301-1031 . . . . . . . . . . . . . . . . . . . . . . . . .32, 173 9301-1083 . . . . . . . . . . . . . . . . . . . . . . . . . . . .153 9301-1104 . . . . . . . . . . . . . . . . . . . . . . . . . . . .209 9301-1161 . . . . . . . . . . . . . . . . . . . . . . . .694, 696 9301-1185 . . . . . . . . . . . . . . . . . . . . . . . . . . . .170 9301-1337 . . . . . . . . . . . . . . . . . . . . . . . .460, 486 9301-1377 . . . . . . . . . . . . . . . . . . . . . . . . . . . .468 9301-1378 . . . . . . . . . . . . . . . . . . . . . . . . . . . .468 9301-1379 . . . . . . . . . . . . . . . . . . . . . . . . . . . .468 9301-1388 . . . . . . . . . . . . . . . . . . . . . . . .488, 496 9301-1419 . . . . . . . . . . . . . . . . . . . . . . . . . . . .468 9301-1420 . . . . . . . . . . . . . . . . . . . . . . . . . . . .464 9301-1421 . . . . . . . . . . . . . . . . . . . . . . . . . . . .464 9301-1425 . . . . . . . . . . . . . . . . . . . . . . . . . . . .468 9301-1450 . . . . . . . . . . . . . . . . . . . . . . . . . . . .464 9301-6341 . . . . . . . . . . . . . . . . . . . . . . . . . . . .465 9301-6342 . . . . . . . . . . . . . . . . . . . . . . . . . . . .465 930990-906 . . . . . . . . . . . . . . . . . . . . . . .539, 559 9310-4828 . . . . . . . . . . . . . . . . . . . . . . . .205, 214 931975-906 . . . . . . . . . . . . . . . . . . . . . . .540, 557 931975-936 . . . . . . . . . . . . . . . . . . . . . . .540, 557 9320-5710 . . . . . . . . . . . . . . . . . . . . . . . . . . . .108 9320-5713 . . . . . . . . . . . . . . . . . . . . . . . . . . . .109 9320-5790 . . . . . . . . . . . . . . . . . . . . . . . . . . . .109 9320-5798 . . . . . . . . . . . . . . . . . . . . . . . . . . . .108 933975-906 . . . . . . . . . . . . . . . . . . . . . . .540, 557 933975-936 . . . . . . . . . . . . . . . . . . . . . . .540, 557 935967-912 . . . . . . . . . . . . . . . . . . . . . . .539, 559 945750-302 . . . . . . . . . . . . . . . . . . . . . . . . . . .593 945750-902 . . . . . . . . . . . . . . . . . . . . . . . . . . .593 946975-906 . . . . . . . . . . . . . . . . . . . . . . . . . . .539 960967-912 . . . . . . . . . . . . . . . . . . . . . . . . . . .539 961400-302 . . . . . . . . . . . . . . . . . . . . . . . . . . .598 961600-906 . . . . . . . . . . . . . . . . . . . . . . . . . . .539 961753-906 . . . . . . . . . . . . . . . . . . . . . . . . . . .539 961967-906 . . . . . . . . . . . . . . . . . . . . . . . . . . .539 963400-902 . . . . . . . . . . . . . . . . . . . . . . . . . . .598 963600-906 . . . . . . . . . . . . . . . . . . . . . . . . . . .539 963954-312 . . . . . . . . . . . . . . . . . . . . . . .539, 559 963967-912 . . . . . . . . . . . . . . . . . . . . . . . . . . .539 965600-906 . . . . . . . . . . . . . . . . . . . . . . . . . . .539 966400-902 . . . . . . . . . . . . . . . . . . . . . . . . . . .598 966735-902 . . . . . . . . . . . . . . . . . . . . . . . . . . .539 966954-302 . . . . . . . . . . . . . . . . . . . . . . . . . . .539 966967-912 . . . . . . . . . . . . . . . . . . . . . . . . . . .539 970050-906 . . . . . . . . . . . . . . . . . . . . . . .540, 553 970100-906 . . . . . . . . . . . . . . . . . . . . . . .540, 553 970150-906 . . . . . . . . . . . . . . . . . . . . . . .540, 553 971700-906 . . . . . . . . . . . . . . . . . . . . . . . . . . .539 973700-906 . . . . . . . . . . . . . . . . . . . . . . .540, 557 973700-936 . . . . . . . . . . . . . . . . . . . . . . .540, 557 975700-906 . . . . . . . . . . . . . . . . . . . . . . .540, 557 975700-936 . . . . . . . . . . . . . . . . . . . . . . .540, 557 990967-206 . . . . . . . . . . . . . . . . . . . . . . . . . . .539 990967-312 . . . . . . . . . . . . . . . . . . . . . . . . . . .539 990967-912 . . . . . . . . . . . . . . . . . . . . . . . . . . .539 992750-902 . . . . . . . . . . . . . . . . . . . . . . . . . . .593 993400-902 . . . . . . . . . . . . . . . . . . . . . . . . . . .598 993700-912 . . . . . . . . . . . . . . . . . . . . . . . . . . .539 993850-902 . . . . . . . . . . . . . . . . . . . . . . . . . . .593 993967-312 . . . . . . . . . . . . . . . . . . . . . . . . . . .539 993967-912 . . . . . . . . . . . . . . . . . . . . . . . . . . .539 ACR . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5758 BACR . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .58 BHT-2 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .58 BHT-4 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .58 BMSR-1 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .55 BMT-2 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .55 BMT-4 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .55 BOT-2 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .57, 71 BOT-4 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .57 G1072-20008 . . . . . . . . . . . . . . . . . . . . .206207 G1099-20130 G1099-20132 G1099-20133 G1099-20136 G1099-60104 G1099-60106 G1099-60170 G1099-60177 G1099-80001 G1099-80053 G1103-60001 G1103-61611 G1120-60006 G1120-62301 G1120-68707 G1120-68708 G1303-60001 G1311-60003 G1311-60009 G1311-60012 G1311-67304 G1312-60010 G1312-60012 G1312-67300 G1312-67301 G1312-67302 G1312-67304 G1312-67305 G1312-68706 G1312-87330 G1313-27302 G1313-44500 G1313-44502 G1313-44503 G1313-60004 G1313-68705 G1313-68709 G1313-68711 G1313-68713 G1313-87101 G1313-87102 G1313-87103 G1313-87201 G1313-87202 G1313-87301 G1313-87304 G1313-87305 G1313-87307 G1313-87308 G1314-60081 G1314-60082 G1314-60083 G1314-60086 G1314-60100 G1314-65050 G1314-65051 G1314-65052 G1314-65053 . . . . . . . . . . . . . . . . . . . . . . . . .206 . . . . . . . . . . . . . . . . . . . . . . . . .206 . . . . . . . . . . . . . . . . . . . . . . . . .206 . . . . . . . . . . . . . . . . . . . . . . . . .206 . . . . . . . . . . . . . . . . . . . . . . . . .206 . . . . . . . . . . . . . . . . . . . . . . . . .206 . . . . . . . . . . . . . . . . . . . . . . . . .206 . . . . . . . . . . . . . . . . . . . . . . . . .206 . . . . . . . . . . . . . . . . . . . . . . . . .210 . . . . . . . . . . . . . . . . . . . . . . . . .207 . . . . . . . . . . . . . . . . .455, 478, 697 . . . . . . . . . . . . . . . . . . . . . . . . .106 . . . . . . . . . . . . . . . . . . . . . . . . .696 . . . . . . . . . . . . . . . . . . . . . . . . .696 . . . . . . . . . . . . . . . . . . . . . . . . .696 . . . . . . . . . . . . . . . . . . . . . . . . .696 . . . . . . . . . . . . . . . . . . . . . . . . .486 . . . . . . . . . . . . . . . . . . . . . . . . .464 . . . . . . . . . . . . . . . . . . . . .462, 485 . . . . . . . . . . . . . . . . . . . . .463, 485 . . . . . . . . . . . . . . . . . . . . . . . . .472 . . . . . . . . . . . . . . . . . . . . . . . . .463 . . . . . . . . . . . . . . . . . . . . . . . . .463 . . . . . . . . . . . . . . . . . . . . . . . . .472 . . . . . . . . . . . . . . . . . . . . . . . . .464 . . . . . . . . . . . . . . . . . . . . . . . . .472 . . . . . . . . . . . . . . . . . . . . . . . . .472 . . . . . . . . . . . . . . . . . . . . .445, 459 . . . . . . . . . . . . . . . . . . . . . . . . .464 . . . . . . . . . . . . . . . . . . . . . . . . .464 . . . . . . . . . . . . . . . . . . . . . . . . .468 . . . . . . . . . . . . . . . . . . . . . . . . .468 . . . . . . . . . . . . . . . . . . . . . . . . .468 . . . . . . . . . . . . . . . . . . . . . . . . .468 . . . . . . . . . . . . . . . . . . . . . . . . .468 . . . . . . . . . . . . . . . . . . . . . . . . .466 . . . . . . . . . . . . . . . . . . . . . . . . .466 . . . . . . . . . . . . . . . . . . . . . . . . .467 . . . . . . . . . . . . . . . . . . . . . . . . .467 . . . . . . . . . . . . . . . . . . . . . . . . .467 . . . . . . . . . . . . . . . . . . . . . . . . .467 . . . . . . . . . . . . . . . . . . . . . . . . .467 . . . . . . . . . . . . . . . . . . . . . . . . .467 . . . . . . . . . . . . . . . . . . . . . . . . .467 . . . . . . . . . . . . . . . . . . . . .445, 467 . . . . . . . . . . . . . . . . .445, 459, 471 . . . . . . . . . . . . . . . . .445, 459, 471 . . . . . . . . . . . . . . . . . . . . . . . . .467 . . . . . . . . . . . . . . . . . . . . . . . . .467 . . . . . . . . . . . . . . . . .456, 474, 476 . . . . . . . . . . . . . . . . .456, 474, 476 . . . . . . . . . . . . . . . . .456, 474, 476 . . . . . . . . . . . . . . . . .456, 474475 . . . . . . . . . . . . . . . . . . . . .455, 474 . . . . . . . . . . . . . . . . . . . . . . . . .475 . . . . . . . . . . . . . . . . . . . . . . . . .475 . . . . . . . . . . . . . . . . . . . . . . . . .476 . . . . . . . . . . . . . . . . . . . . . . . . .476
Indices
741
Part Number Index

G1314-65054 G1314-65056 G1314-65061 G1314-65062 G1314-65063 G1314-65064 G1314-65065 G1314-65066 G1314-68705 G1315-27705 G1315-45001 G1315-45003 G1315-60011 G1315-60012 G1315-60015 G1315-60016 G1315-60017 G1315-60018 G1315-67301 G1315-67302 G1315-68703 G1315-68708 G1315-68710 G1315-68711 G1315-68712 G1315-68713 G1315-68714 G1315-68715 G1315-80001 G1315-80003 G1315-80004 G1315-84901 G1315-87302 G1315-87305 G1315-87306 G1315-87311 G1315-87312 G1315-87321 G1315-87325 G1316-68707 G1316-87300 G1316-87303 G1321-60005 G1321-60007 G1322-67300 G1322-68705 G1328-87600 G1329-60001 G1329-68715 G1329-80001 G1329-87101 G1329-87103 G1329-87300 G1361-22402 G1361-23204 G1361-60002 G1361-60012 G1361-67300 . . . . . . . . . . . . . . . . . . . . . . . . .476 . . . . . . . . . . . . . . . . . . . . . . . . .476 . . . . . . . . . . . . . . . . . . . . . . . . .475 . . . . . . . . . . . . . . . . . . . . . . . . .475 . . . . . . . . . . . . . . . . . . . . . . . . .475 . . . . . . . . . . . . . . . . . . . . . . . . .475 . . . . . . . . . . . . . . . . . . . . . . . . .475 . . . . . . . . . . . . . . . . . . . . . . . . .475 . . . . . . . . . . . . . . . . . . . . . . . . .474 . . . . . . . . . . . . . . . . . . . . . . . . .481 . . . . . . . . . . . . . . . . . . . . . . . . .478 . . . . . . . . . . . . . . . . . . . . .458, 480 . . . . . . . . . . . . . . . . .456, 477, 479 . . . . . . . . . . . . . . . . .456, 477478 . . . . . . . . . . . . . . . . .456, 477, 479 . . . . . . . . . . . . . . . . . . . . .456, 481 . . . . . . . . . . . . . . . . . . . . .456, 481 . . . . . . . . . . . . . . . . . . . . .456, 481 . . . . . . . . . . . . . . . . . . . . . . . . .481 . . . . . . . . . . . . . . . . . . . . . . . . .481 . . . . . . . . . . . . . . . . . . . . .472, 480 . . . . . . . . . . . . . . . . .472473, 480 . . . . . . . . . . . . . . . . . . . . .478479 . . . . . . . . . . . . . . . . . . . . . . . . .478 . . . . . . . . . . . . . . . . . . . . . . . . .478 . . . . . . . . . . . . . . . . . . . . . . . . .479 . . . . . . . . . . . . . . . . .456, 477, 480 . . . . . . . . . . . . . . . . . . . . . . . . .480 . . . . . . . . . . . . . . . . . . . . . . . . .480 . . . . . . . . . . . . . . . . . . . . . . . . .481 . . . . . . . . . . . . . . . . . . . . . . . . .481 . . . . . . . . . . . . . . . . . . . . .478480 . . . . . . . . . . . . . . . . . . . . . . . . .478 . . . . . . . . . . . . . . . . . . . . . . . . .481 . . . . . . . . . . . . . . . . . . . . . . . . .479 . . . . .445, 459, 476, 478479, 481 . . . . . . . . . . . . .445, 459, 478479 . . . . . . . . . . . . . . . . . . . . . . . . .478 . . . . . . . . . . . . . . . . . . . . . . . . .479 . . . . . . . . . . . . . . . . . . . . . . . . .471 . . . . . . . . . . . . . . . . .445, 459, 471 . . . . . . . . . . . . . . . . .445, 459, 471 . . . . . . . . . . . . . . . . . . . . .456, 481 . . . . . . . . . . . . . . . . . . . . .456, 481 . . . . . . . . . . . . . . . . . . . . .460, 464 . . . . . . . . . . . . . . . . . . . . . . . . .460 . . . . . . . . . . . . . . . . . . . . .445, 459 . . . . . . . . . . . . . . . . . . . . . . . . .468 . . . . . . . . . . . . . . . . . . . . . . . . .469 . . . . . . . . . . . . . . . . . . . . . . . . .469 . . . . . . . . . . . . . . . . . . . . .469, 472 . . . . . . . . . . . . . . . . . . . . . . . . .469 . . . . . . . . . . . . . . . . . . . . .445, 459 . . . . . . . . . . . . . . . . . . . . .455, 465 . . . . . . . . . . . . . . . . . . . . . . . . .465 . . . . . . . . . . . . . . . . . . . . . . . . .465 . . . . . . . . . . . . . . . . . . . . . . . . .465 . . . . . . . . . . . . . . . . . . . . . . . . .465 G1361-67301 G1361-67302 G1361-67303 G1361-67304 G1361-67305 G1361-67306 G1362-68709 G1362-87300 G1362-87301 G1364-68711 G1364-68712 G1364-68730 G1364-84501 G1364-84502 G1364-84503 G1364-84504 G1364-84505 G1364-84506 G1367-60001 G1367-68705 G1367-87101 G1367-87200 G1367-87300 G1375-87300 G1375-87301 G1375-87302 G1375-87303 G1375-87304 G1375-87305 G1375-87306 G1375-87307 G1375-87308 G1375-87309 G1375-87310 G1375-87311 G1375-87312 G1375-87315 G1375-87317 G1375-87400 G1376-60003 G1376-68705 G1377-60002 G1377-87101 G1377-87200 G1377-87300 G1377-87301 G1379-60001 G1379-67300 G1512-60530 G1530-60560 G1530-60570 G1530-60590 G1530-60600 G1530-60610 G1530-60630 G1530-60930 G1531-20690 G1531-20700 . . . . . . . . . . . . . . . . . . . . . . . . .465 . . . . . . . . . . . . . . . . . . . . . . . . .465 . . . . . . . . . . . . . . . . . . . . . . . . .465 . . . . . . . . . . . . . . . . . . . . . . . . .465 . . . . . . . . . . . . . . . . . . . . . . . . .465 . . . . . . . . . . . . . . . . . . . . . . . . .465 . . . . . . . . . . . . . . . . . . . . . . . . .482 . . . . . . . . . . . . . . . . . . . . . . . . .482 . . . . . . . . . . . . . . . . . . . . . . . . .482 . . . . . . . . . . . . . . . . . . . . . . . . .465 . . . . . . . . . . . . . . . . . . . . . . . . .465 . . . . . . . . . . . . . . . . . . . . . . . . .465 . . . . . . . . . . . . . . . . . . . . . . . . .465 . . . . . . . . . . . . . . . . . . . . . . . . .465 . . . . . . . . . . . . . . . . . . . . . . . . .465 . . . . . . . . . . . . . . . . . . . . . . . . .465 . . . . . . . . . . . . . . . . . . . . . . . . .465 . . . . . . . . . . . . . . . . . . . . . . . . .465 . . . . . . . . . . . . . . . . . . . . . . . . .469 . . . . . . . . . . . . . . . . . . . . . . . . .469 . . . . . . . . . . . . . . . . . . . . . . . . .469 . . . . . . . . . . . . . . . . . . . . . . . . .469 . . . . . . . . . . . . . . . . . . . . . . . . .469 . . . . . . . . . . . . . . . . . . . . . . . . .470 . . . . . . . . . . . . . . . . . . . . . . . . .472 . . . . . . . . . . . . . . . . . . . . . . . . .472 . . . . . . . . . . . . . . . . . . . . .472473 . . . . . . . . . . . . . . . . . . . . . . . . .472 . . . . . . . . . . . . . . . . . . . . . . . . .473 . . . . . . . . . . . . . . . . . . . . . . . . .473 . . . . . . . . . . . . . . . . . . . . .472473 . . . . . . . . . . . . . . . . . . . . . . . . .473 . . . . . . . . . . . . . . . . . . . . .472473 . . . . . . . . . . . . . . . . . . . . . . . . .472 . . . . . . . . . . . . . . . . . . . . . . . . .473 . . . . . . . . . . . . . . . . . . . . . . . . .473 . . . . . . . . . . . . . . . . . . . . . . . . .470 . . . . . . . . . . . . . . . . . . . . . . . . .470 . . . . . . . . . . . . . . . . . . . . . . . . .472 . . . . . . . . . . . . . . . . . . . . . . . . .464 . . . . . . . . . . . . . . . . . . . . . . . . .464 . . . . . . . . . . . . . . . . . . . . . . . . .469 . . . . . . . . . . . . . . . . . . . . . . . . .470 . . . . . . . . . . . . . . . . . . . . . . . . .470 . . . . . . . . . . . . . . . . . . . . . . . . .470 . . . . . . . . . . . . . . . . . . . . . . . . .470 . . . . . . . . . . . . . . . . . . . . . . . . .460 . . . . . . . . . . . . . . . . . . . . . . . . .460 . . . . . . . . . . . . . . . . . . . . . . . . .106 . . . . . . . . . . . . . . . . . . . . . . . . .106 . . . . . . . . . . . . . . . . . . . . .104105 . . . . . . . . . . . . . . . . . . . . . . . . .106 . . . . . . . . . . . . . . . . . . . . . . . . .106 . . . . . . . . . . . . . . . . . . . . . . . . .106 . . . . . . . . . . . . . . . . . . . . . . . . .106 . . . . . . . . . . . . . . . . . . . . . . . . .106 . . . . . . . . . . . . . . . . . . . . . . . . .182 . . . . . . . . . . . . . . . . . . . . . . . . .182 G1531-20740 . . . . . . . . . . . . . . . . . . . . . . . . .182 G1531-40020 . . . . . . . . . . . . . . . . . . . . . . . . .184 G1531-60680 . . . . . . . . . . . . . . . . . . . . . . . . .183 G1531-60690 . . . . . . . . . . . . . . . . . . . . . . . . .182 G1531-80560 . . . . . . . . . . . . . . . . . . . . .182, 184 G1531-80620 . . . . . . . . . . . . . . . . . . . . . . . . .182 G1532-20710 . . . . . . . . . . . . . . . . . . . . . . . . .185 G1532-60675 . . . . . . . . . . . . . . . . . . . . . . . . .185 G1533-80565 . . . . . . . . . . . . . . . . . . . . . . . . .187 G1534-20530 . . . . . . . . . . . . . . . . . . . . . . . . .184 G1534-20590 . . . . . . . . . . . . . . . . . . . . . . . . .184 G1534-40020 . . . . . . . . . . . . . . . . . . . . . . . . .184 G1534-40030 . . . . . . . . . . . . . . . . . . . . . . . . .184 G1534-60570 . . . . . . . . . . . . . . . . . . . . . . . . .184 G1534-60610 . . . . . . . . . . . . . . . . . . . . . . . . .184 G1534-80500 . . . . . . . . . . . . . . . . . . . . . . . . .184 G1534-80510 . . . . . . . . . . . . . . . . . . . . . . . . .184 G1534-80540 . . . . . . . . . . . . . . . . . . . . . . . . .184 G1535-60600 . . . . . . . . . . . . . . . . . . . . . . . . .187 G1544-20590 . . . . . . . . . . . . . . . . . . . . . . . . .176 G1544-60575 . . . . . . . . . . . . . . . . . . . . . . . . .175 G1544-60585 . . . . . . . . . . . . . . . . . . . . . . . . .175 G1544-60610 . . . . . . . . . . . . . . . . . . . . . . . . .176 G1544-80530 . . . . . . . . . . . . . . . . . . . . . . . . .176 G1544-80550 . . . . . . . . . . . . . . . . . . . . . . . . .176 G1544-80580 . . . . . . . . . . . . . . . . . . . . . . . . .175 G1544-80700 . . . . . . . . . . . . . . . . . . . . . . . . .158 G1544-80730 . . . . . . . . . . . . . . . . . . . . . . . . .158 G1545-80520 . . . . . . . . . . . . . . . . . . . . .178179 G1553A . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .104 G1580-60575 . . . . . . . . . . . . . . . . . . . . . . . . .175 G1580-60585 . . . . . . . . . . . . . . . . . . . . . . . . .175 G1600-23223 . . . . . . . . . . . . . . . . . . . . . . . . .656 G1600-60002 . . . . . . . . . . . . . . . . . . . . . . . . .659 G1600-60007 . . . . . . . . . . . . . . . . . . . . . . . . .656 G1600-60013 . . . . . . . . . . . . . . . . . . . . . . . . .657 G1600-60027 . . . . . . . . . . . . . . . . . . . . . . . . .655 G1600-60132 . . . . . . . . . . . . . . . . . . . . . . . . .662 G1600-60150 . . . . . . . . . . . . . . . . . . . . . . . . .659 G1600-60210 . . . . . . . . . . . . . . . . . . . . . . . . .659 G1600-60211 . . . . . . . . . . . . . . . . . . . . . . . . .661 G1600-60230 . . . . . . . . . . . . . . . . . . . . . . . . .659 G1600-60232 . . . . . . . . . . . . . . . . . . . . . . . . .662 G1600-60310 . . . . . . . . . . . . . . . . . . . . . . . . .659 G1600-60311 . . . . . . . . . . . . . . . . . . . . . . . . .661 G1600-60330 . . . . . . . . . . . . . . . . . . . . . . . . .659 G1600-60332 . . . . . . . . . . . . . . . . . . . . . . . . .662 G1600-60400 . . . . . . . . . . . . . . . . . . . . .657, 659 G1600-60411 . . . . . . . . . . . . . . . . . . . . . . . . .661 G1600-60419 . . . . . . . . . . . . . . . . . . . . . . . . .663 G1600-61132 . . . . . . . . . . . . . . . . . . . . . . . . .662 G1600-61211 . . . . . . . . . . . . . . . . . . . . . . . . .661 G1600-61219 . . . . . . . . . . . . . . . . . . . . . . . . .663 G1600-61232 . . . . . . . . . . . . . . . . . . . . .662, 667 G1600-61239 . . . . . . . . . . . . . . . . . . . . . . . . .663 G1600-61311 . . . . . . . . . . . . . . . . . . . . . . . . .661 G1600-61332 . . . . . . . . . . . . . . . . . . . . . . . . .662 G1600-61411 . . . . . . . . . . . . . . . . . . . . . . . . .661
Indices
742
Part Number Index

G1600-61419 . . . . . . . . . . . . . . . . . . . . . . . . .663 G1600-62132 . . . . . . . . . . . . . . . . . . . . . . . . .662 G1600-62211 . . . . . . . . . . . . . . . . .661, 666, 668 G1600-62232 . . . . . . . . . . . . . . . . . . . . . . . . .662 G1600-62311 . . . . . . . . . . . . . . . . . . . . .661, 667 G1600-62318 . . . . . . . . . . . . . . . . . . . . .663664 G1600-62332 . . . . . . . . . . . . . . . . . . . . . . . . .662 G1600-62402 . . . . . . . . . . . . . . . . . . . . . . . . .656 G1600-62411 . . . . . . . . . . . . . . . . . . . . . . . . .661 G1600-62700 . . . . . . . . . . . . . . . . . . . . .656, 659 G1600-63200 . . . . . . . . . . . . . . . . . . . . . . . . .655 G1600-63211 . . . . . . . . . . . . . . . . . . . . . . . . .661 G1600-63311 . . . . . . . . . . . . . . . . . . . . . . . . .661 G1600-63411 . . . . . . . . . . . . . . . . . . . . . . . . .661 G1600-64211 . . . . . . . . . . . . . . . . . . . . .661, 667 G1600-64232 . . . . . . . . . . . . . . . . . . . . . . . . .662 G1600-64311 . . . . . . . . . . . . . . . . . . . . . . . . .661 G1600-64332 . . . . . . . . . . . . . . . . . . . . . . . . .662 G1600-64411 . . . . . . . . . . . . . . . . . . . . . . . . .661 G1600-67201 . . . . . . . . . . . . . . . . . . . . . . . . .656 G1600-67219 . . . . . . . . . . . . . . . . . . . . .657, 663 G1600-67311 . . . . . . . . . . . . . . . . . . . . . . . . .657 G1600-67319 . . . . . . . . . . . . . . . . . . . . .657, 663 G1600-68319 . . . . . . . . . . . . . . . . . . . . .655, 663 G1600-68701 . . . . . . . . . . . . . . . . . . . . . . . . .656 G1600-68706 . . . . . . . . . . . . . . . . . . . . . . . . .658 G1600-68713 . . . . . . . . . . . . . . . . . . . . . . . . .655 G1600-68714 . . . . . . . . . . . . . . . . . . . . . . . . .655 G1600-68715 . . . . . . . . . . . . . . . . . . . . . . . . .655 G1600-68716 . . . . . . . . . . . . . . . . . . . . . . . . .655 G1603A . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .657 G1607-20030 . . . . . . . . . . . . . . . . . . . . . . . . .657 G1607-60000 . . . . . . . . . . . . . . . . . . . . . . . . .657 G1607-60001 . . . . . . . . . . . . . . . . . . . . . . . . .657 G1607-60041 . . . . . . . . . . . . . . . . . . . . . . . . .657 G1607A . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .657 G160U-60419 . . . . . . . . . . . . . . . . . . . . . . . . .663 G160U-61219 . . . . . . . . . . . . . . . . . . . . . . . . .663 G160U-61239 . . . . . . . . . . . . . . . . . . . . . . . . .663 G160U-61419 . . . . . . . . . . . . . . . . . . . . . . . . .663 G1680-63710 . . . . . . . . . . . . . . . . . . . . . . . . .106 G1680-63711 . . . . . . . . . . . . . . . . . . . . . . . . .106 G1680-63715 . . . . . . . . . . . . . . . . . . . . . . . . .106 G1800-20305 . . . . . . . . . . . . . . . . . . . . . . . . .207 G1811-23200 . . . . . . . . . . . . . . . . . . . . . . . . .697 G1820-60104 . . . . . . . . . . . . . . . . . . . . . . . . .686 G1820-60160 . . . . . . . . . . . . . . . . . . . . .673, 682 G1820-60376 . . . . . . . . . . . . . . . . . . . . . . . . .686 G1820-60393 . . . . . . . . . . . . . . . . . . . . . . . . .686 G1820-60399 . . . . . . . . . . . . . . . . . . . . . . . . .686 G1820-60453 . . . . . . . . . . . . . . . . . . . . .673, 682 G1820-65003 . . . . . . . . . . . . . . . . . . . . . . . . .679 G1820-65004 . . . . . . . . . . . . . . . . . . . . . . . . .679 G1820-65007 . . . . . . . . . . . . . . . . . . . . . . . . .684 G1820-65008 . . . . . . . . . . . . . . . . . . . . . . . . .679 G1820-65013 . . . . . . . . . . . . . . . . . . . . . . . . .685 G1820-65015 . . . . . . . . . . . . . . . . . . . . . . . . .684 G1820-65016 . . . . . . . . . . . . . . . . . . . . .670, 680 G1820-65017 G1820-65018 G1820-65019 G1820-65023 G1820-65025 G1820-65026 G1820-65027 G1820-65028 G1820-65030 G1820-65031 G1820-65032 G1820-65035 G1820-65044 G1820-65050 G1820-65052 G1820-65057 G1820-65060 G1820-65061 G1820-65062 G1820-65073 G1820-65074 G1820-65075 G1820-65105 G1820-65110 G1820-65119 G1820-65121 G1820-65138 G1820-65141 G1820-65144 G1820-65163 G1820-65164 G1820-65185 G1820-65198 G1820-65199 G1820-65201 G1820-65214 G1820-65215 G1820-65216 G1820-65220 G1820-65221 G1820-65237 G1820-65238 G1820-65239 G1820-65265 G1820-65276 G1820-65336 G1820-65337 G1820-65338 G1820-65339 G1820-65342 G1820-65343 G1820-65345 G1820-65356 G1820-65357 G1820-65358 G1820-65360 G1820-65361 G1820-65435 . . . . . . . . . . . . . . . . . . . . . . . . .688 . . . . . . . . . . . . . . . . . . . . .678, 688 . . . . . . . . . . . . . . . . . . . . . . . . .688 . . . . . . . . . . . . . . . . . . . . .669, 679 . . . . . . . . . . . . . . . . . . . . .676, 685 . . . . . . . . . . . . . . . . . . . . . . . . .684 . . . . . . . . . . . . . . . . . . . . .670, 679 . . . . . . . . . . . . . . . . . . . . . . . . .684 . . . . . . . . . . . . . . . . . . . . .673, 682 . . . . . . . . . . . . . . . . . . . . . . . . .688 . . . . . . . . . . . . . . . . . . . . . . . . .688 . . . . . . . . . . . . . . . . . . . . . . . . .684 . . . . . . . . . . . . . . . . . . . . . . . . .686 . . . . . . . . . . . . . . . . . . . . . . . . .676 . . . . . . . . . . . . . . . . . . . . .670, 679 . . . . . . . . . . . . . . . . . . . . . . . . .686 . . . . . . . . . . . . . . . . . . . . . . . . .685 . . . . . . . . . . . . . . . . . . . . . . . . .685 . . . . . . . . . . . . . . . . . . . . . . . . .686 . . . . . . . . . . . . . . . . . . . . . . . . .688 . . . . . . . . . . . . . . . . . . . . . . . . .688 . . . . . . . . . . . . . . . . . . . . . . . . .682 . . . . . . . . . . . . . . . . . . . . .673, 683 . . . . . . . . . . . . . . . . . . . . . . . . .686 . . . . . . . . . . . . . . . . . . . . .670, 679 . . . . . . . . . . . . . . . . . . . . . . . . .682 . . . . . . . . . . . . . . . . . . . . .673, 682 . . . . . . . . . . . . . . . . . . . . .673, 682 . . . . . . . . . . . . . . . . . . . . . . . . .679 . . . . . . . . . . . . . . . . . . . . . . . . .684 . . . . . . . . . . . . . . . . . . . . . . . . .684 . . . . . . . . . . . . . . . . . . . . . . . . .684 . . . . . . . . . . . . . . . . . . . . .673, 682 . . . . . . . . . . . . . . . . . . . . . . . . .682 . . . . . . . . . . . . . . . . . . . . . . . . .684 . . . . . . . . . . . . . . . . . . . . .670, 679 . . . . . . . . . . . . . . . . . . . . .670, 680 . . . . . . . . . . . . . . . . . . . . .670, 680 . . . . . . . . . . . . . . . . . . . . .670, 680 . . . . . . . . . . . . . . . . . . . . .670, 680 . . . . . . . . . . . . . . . . . . . . .676, 686 . . . . . . . . . . . . . . . . . . . . .676, 685 . . . . . . . . . . . . . . . . . . . . .676, 685 . . . . . . . . . . . . . . . . . . . . . . . . .685 . . . . . . . . . . . . . . . . . . . . .673, 682 . . . . . . . . . . . . . . . . . . . . . . . . .683 . . . . . . . . . . . . . . . . . . . . .674, 683 . . . . . . . . . . . . . . . . . . . . . . . . .683 . . . . . . . . . . . . . . . . . . . . . . . . .683 . . . . . . . . . . . . . . . . . . . . . . . . .683 . . . . . . . . . . . . . . . . . . . . . . . . .683 . . . . . . . . . . . . . . . . . . . . .673, 683 . . . . . . . . . . . . . . . . . . . . . . . . .682 . . . . . . . . . . . . . . . . . . . . . . . . .684 . . . . . . . . . . . . . . . . . . . . . . . . .686 . . . . . . . . . . . . . . . . . . . . .676, 685 . . . . . . . . . . . . . . . . . . . . . . . . .685 . . . . . . . . . . . . . . . . . . . . .676, 686 G1820-65478 G1820-65479 G1820-65480 G1820-65481 G1820-65482 G1820-65491 G1820-65494 G1820-65497 G1820-65498 G1820-65501 G1820-65502 G1820-65503 G1820-65504 G1820-65505 G1820-65515 G1820-65518 G1820-65520 G1820-65528 G1820-65529 G1820-65533 G1820-80235 G1820-80237 G1820-80386 G1820-80512 G1833-65024 G1833-65025 G1833-65079 G1833-65088 G1833-65089 G1833-65092 G1833-65094 G1833-65096 G1833-65332 G1833-65404 G1833-65405 G1833-65406 G1833-65407 G1833-65408 G1833-65409 G1833-65412 G1833-65413 G1833-65414 G1833-65415 G1833-65416 G1833-65417 G1833-65418 G1833-65419 G1833-65420 G1833-65421 G1833-65422 G1833-65423 G1833-65424 G1833-65425 G1833-65426 G1833-65427 G1833-65428 G1833-65429 G1833-65430 . . . . . . . . . . . . . . . . . . . . .673, 683 . . . . . . . . . . . . . . . . . . . . .670, 680 . . . . . . . . . . . . . . . . . . . . .676, 686 . . . . . . . . . . . . . . . . . . . . .676, 686 . . . . . . . . . . . . . . . . . . . . .674, 684 . . . . . . . . . . . . . . . . . . . . .674, 683 . . . . . . . . . . . . . . . . . . . . . . . . .686 . . . . . . . . . . . . . . . . . . . . . . . . .685 . . . . . . . . . . . . . . . . . . . . . . . . .685 . . . . . . . . . . . . . . . . . . . . . . . . .685 . . . . . . . . . . . . . . . . . . . . . . . . .685 . . . . . . . . . . . . . . . . . . . . .674, 684 . . . . . . . . . . . . . . . . . . . . .674, 684 . . . . . . . . . . . . . . . . . . . . .670, 680 . . . . . . . . . . . . . . . . . . . . .670, 680 . . . . . . . . . . . . . . . . . . . . .674, 683 . . . . . . . . . . . . . . . . . . . . .674, 683 . . . . . . . . . . . . . . . . . . . . . . . . .685 . . . . . . . . . . . . . . . . . . . . . . . . .685 . . . . . . . . . . . . . . . . . . . . .674, 683 . . . . . . . . . . . . . . . . . . . . . . . . .684 . . . . . . . . . . . . . . . . . . . . . . . . .684 . . . . . . . . . . . . . . . . . . . . . . . . .687 . . . . . . . . . . . . . . . . . . . . . . . . .684 . . . . . . . . . . . . . . . . . . . . . . . . .677 . . . . . . . . . . . . . . . . . . . . . . . . .677 . . . . . . . . . . . . . . . . . . . . . . . . .676 . . . . . . . . . . . . . . . . . . . . . . . . .677 . . . . . . . . . . . . . . . . . . . . . . . . .677 . . . . . . . . . . . . . . . . . . . . . . . . .676 . . . . . . . . . . . . . . . . . . . . . . . . .677 . . . . . . . . . . . . . . . . . . . . . . . . .677 . . . . . . . . . . . . . . . . . . . . . . . . .678 . . . . . . . . . . . . . . . . . . . . . . . . .677 . . . . . . . . . . . . . . . . . . . . . . . . .676 . . . . . . . . . . . . . . . . . . . . . . . . .675 . . . . . . . . . . . . . . . . . . . . . . . . .676 . . . . . . . . . . . . . . . . . . . . . . . . .676 . . . . . . . . . . . . . . . . . . . . . . . . .675 . . . . . . . . . . . . . . . . . . . . . . . . .678 . . . . . . . . . . . . . . . . . . . . . . . . .677 . . . . . . . . . . . . . . . . . . . . . . . . .669 . . . . . . . . . . . . . . . . . . . . . . . . .675 . . . . . . . . . . . . . . . . . . . . . . . . .675 . . . . . . . . . . . . . . . . . . . . . . . . .677 . . . . . . . . . . . . . . . . . . . . . . . . .677 . . . . . . . . . . . . . . . . . . . . . . . . .675 . . . . . . . . . . . . . . . . . . . . . . . . .678 . . . . . . . . . . . . . . . . . . . . . . . . .675 . . . . . . . . . . . . . . . . . . . . . . . . .675 . . . . . . . . . . . . . . . . . . . . . . . . .675 . . . . . . . . . . . . . . . . . . . . . . . . .675 . . . . . . . . . . . . . . . . . . . . . . . . .675 . . . . . . . . . . . . . . . . . . . . . . . . .674 . . . . . . . . . . . . . . . . . . . . . . . . .674 . . . . . . . . . . . . . . . . . . . . . . . . .669 . . . . . . . . . . . . . . . . . . . . . . . . .678 . . . . . . . . . . . . . . . . . . . . . . . . .674
Indices
743
Part Number Index

G1833-65432 . . . . . . . . . . . . . . . . . . . . . . . . .675 G1833-65462 . . . . . . . . . . . . . . . . . . . . .673, 682 G1833-65463 . . . . . . . . . . . . . . . . . . . . . . . . .674 G1833-65464 . . . . . . . . . . . . . . . . . . . . . . . . .674 G1833-65475 . . . . . . . . . . . . . . . . . . . . . . . . .673 G1833-65476 . . . . . . . . . . . . . . . . . . . . .673, 682 G1833-65497 . . . . . . . . . . . . . . . . . . . . . . . . .676 G1833-65498 . . . . . . . . . . . . . . . . . . . . . . . . .676 G1833-65499 . . . . . . . . . . . . . . . . . . . . . . . . .677 G1833-65500 . . . . . . . . . . . . . . . . . . . . . . . . .677 G1833-65501 . . . . . . . . . . . . . . . . . . . . . . . . .677 G1833-65502 . . . . . . . . . . . . . . . . . . . . . . . . .677 G1833-65503 . . . . . . . . . . . . . . . . . . . . . . . . .677 G1833-65505 . . . . . . . . . . . . . . . . . . . . . . . . .676 G1883-65079 . . . . . . . . . . . . . . . . . . . . . . . . .686 G1946-20213 . . . . . . . . . . . . . . . . . . . . . . . . .483 G1946-60180 . . . . . . . . . . . . . . . . . . . . . . . . .483 G1946-80009 . . . . . . . . . . . . . . . . . . . . . . . . .483 G1946-80047 . . . . . . . . . . . . . . . . . . . . . .61, 483 G1947-20029 . . . . . . . . . . . . . . . . . . . . . . . . .483 G1999-80410 . . . . . . . . . . . . . . . . . . . . . . . . . .61 G2080AA . . . . . . . . . . . . . . . . . . . . . . . . . . . .108 G2100A . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .104 G2101A . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .104 G2104A . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .104 G2105A . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .104 G2108A . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .104 G2250-04500 . . . . . . . . . . . . . . . . . . . . . . . . .470 G2250-04501 . . . . . . . . . . . . . . . . . . . . . . . . .470 G2250-04502 . . . . . . . . . . . . . . . . . . . . . . . . .470 G2250-04503 . . . . . . . . . . . . . . . . . . . . . . . . .470 G2250-04504 . . . . . . . . . . . . . . . . . . . . . . . . .470 G2250-23200 . . . . . . . . . . . . . . . . . . . . . . . . .470 G2250-24500 . . . . . . . . . . . . . . . . . . . . . . . . .470 G2250-24501 . . . . . . . . . . . . . . . . . . . . . . . . .470 G2250-47100 . . . . . . . . . . . . . . . . . . . . . . . . .470 G2250-87300 . . . . . . . . . . . . . . . . . . . . . . . . .470 G2350-61090 . . . . . . . . . . . . . . . . . . . . . . . . .196 G2397-20540 . . . . . . . . . . . . . . . . . . . . . . . . .194 G2397-80520 . . . . . . . . . . . . . . . . . . . . . . . . .194 G2421A . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .483 G2423A . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .483 G2424A . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .483 G2425A . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .483 G2426A . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .483 G2427A . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .483 G2428A . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .483 G2431A . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .483 G2432A . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .483 G2617-20510 . . . . . . . . . . . . . . . . . . . . . . . . .177 G2617-60506 . . . . . . . . . . . . . . . . . . . . . . . . .177 G2617-60507 . . . . . . . . . . . . . . . . . . . . . . . . .177 G2617-60508 . . . . . . . . . . . . . . . . . . . . . . . . .177 G2617-60510 . . . . . . . . . . . . . . . . . . . . . . . . .177 G2617-80540 . . . . . . . . . . . . . . . . . . . . . . . . .177 G2618-80500 . . . . . . . . . . . . . . . . . . . . . . . . .177 G2619-60501 . . . . . . . . . . . . . . . . . . . . . . . . .177 G2860A . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .212 G3138-65000 . . . . . . . . . . . . . . . . . . . . .672, 681 G3138-65001 . . . . . . . . . . . . . . . . . . . . .672, 681 G3138-65002 . . . . . . . . . . . . . . . . . . . . .672, 681 G3138-65102 . . . . . . . . . . . . . . . . . . . . .672, 681 G3138-65103 . . . . . . . . . . . . . . . . . . . . .672, 681 G3138-65104 . . . . . . . . . . . . . . . . . . . . .672, 681 G3138-65117 . . . . . . . . . . . . . . . . . . . . .672, 681 G3138-65118 . . . . . . . . . . . . . . . . . . . . .672, 681 G3138-65119 . . . . . . . . . . . . . . . . . . . . .672, 681 G3138-65121 . . . . . . . . . . . . . . . . . . . . .672, 681 G3138-65122 . . . . . . . . . . . . . . . . . . . . .672, 681 G3138-65125 . . . . . . . . . . . . . . . . . . . . .672, 681 G3138-65126 . . . . . . . . . . . . . . . . . . . . .672, 681 G3160-65300 . . . . . . . . . . . . . . . . . . . . .671, 680 G3160-65301 . . . . . . . . . . . . . . . . . . . . .671, 680 G3160-65302 . . . . . . . . . . . . . . . . . . . . .671, 680 G3160-65303 . . . . . . . . . . . . . . . . . . . . .671, 680 G3160-65304 . . . . . . . . . . . . . . . . . . . . .671, 680 G3160-65305 . . . . . . . . . . . . . . . . . . . . .671, 680 G3160-65306 . . . . . . . . . . . . . . . . . . . . .671, 680 G3160-65307 . . . . . . . . . . . . . . . . . . . . .671, 680 G3160-65308 . . . . . . . . . . . . . . . . . . . . .671, 680 G3160-65309 . . . . . . . . . . . . . . . . . . . . .671, 680 G3160-65315 . . . . . . . . . . . . . . . . . . . . .671, 680 G3160-65317 . . . . . . . . . . . . . . . . . . . . .671, 680 GC-1 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .67 GC-2 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .67 GC-2-I . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .67 GC-3 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .67 GC-4 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .67 GMSR . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5455 GMT-2-HP . . . . . . . . . . . . . . . . . . . . . . . . . .54, 71 GMT-2GC-HP . . . . . . . . . . . . . . . . . . . . . . . . . .54 GMT-4-HP . . . . . . . . . . . . . . . . . . . . . . . . . . . . .54 GMT-4GC-HP . . . . . . . . . . . . . . . . . . . . . . . . . .54 HCRMS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .61 HFM-1000 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .79 HFM-1000-2 . . . . . . . . . . . . . . . . . . . . . . . . . . .79 HFM-420 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .79 HFM-570 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .79 HFM-570-FC . . . . . . . . . . . . . . . . . . . . . . . . . . .79 HFM-650 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .79 HFM-650-FC . . . . . . . . . . . . . . . . . . . . . . . . . . .79 HFM-BULB . . . . . . . . . . . . . . . . . . . . . . . . . . . .79 HMT200-2 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .61 HMT200-4 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .61 HT200-2 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .57 HT200-4 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .57 HT3-2 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .58 HT3-4 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .58 HVF-500 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .77 HVF-500-2 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .77 IOT-2-HP . . . . . . . . . . . . . . . . . . . . . . . . . . .56, 71 IOT-4-HP . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .56 LGMT-2-HP . . . . . . . . . . . . . . . . . . . . . . . . . . . .54 LGMT-4-HP . . . . . . . . . . . . . . . . . . . . . . . . . . . .54 LIOT-2 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .56 LIOT-4 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .56 MC-1 . . . . . . . . . . . . . . . . . .5153, 5658, 61, 70 MSR-1 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .51, 55 MSR-2 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .52, 55 MT120-2 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .51 MT120-2-D . . . . . . . . . . . . . . . . . . . . . . . . . . . .52 MT120-2-S . . . . . . . . . . . . . . . . . . . . . . . . . . . .53 MT120-4 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .51 MT120-4-D . . . . . . . . . . . . . . . . . . . . . . . . . . . .52 MT120-4-S . . . . . . . . . . . . . . . . . . . . . . . . . . . .53 MT200-2 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .51 MT200-2-D . . . . . . . . . . . . . . . . . . . . . . . . . . . .52 MT200-2-S . . . . . . . . . . . . . . . . . . . . . . . . . . . .53 MT200-4 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .51 MT200-4-D . . . . . . . . . . . . . . . . . . . . . . . . . . . .52 MT200-4-S . . . . . . . . . . . . . . . . . . . . . . . . . . . .53 MT400-2 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .51 MT400-2-D . . . . . . . . . . . . . . . . . . . . . . . . . . . .52 MT400-2-S . . . . . . . . . . . . . . . . . . . . . . . . . . . .53 MT400-4 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .51 MT400-4-D . . . . . . . . . . . . . . . . . . . . . . . . . . . .52 MT400-4-S . . . . . . . . . . . . . . . . . . . . . . . . . . . .53 OT1-2 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .56 OT1-4 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .56 OT3-2 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .61 OT3-4 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .61 R2D2-I-P . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .67 R2D2-ORINGS . . . . . . . . . . . . . . . . . . . . . . . . .67 R2D2-P . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .67 RBC-D-P . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .67 RBC-P . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .67 RBCD-P-2I/3 . . . . . . . . . . . . . . . . . . . . . . . . . .67 RBCDP-GC2/I . . . . . . . . . . . . . . . . . . . . . . . . . .67 RDT-1020 . . . . . . . . . . . . . . . . . . . . . . . . . .7071 RDT-1023 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .70 RFT-2500 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .89 RFT-5100 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .89 RFT-5300 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .89 RMP-5005 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .89 RMSH-2 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .62 RMSH-4 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .62 RMSHY-2 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .62 RMSHY-4 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .62 RMSN-2 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .62 RMSN-4 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .62 RQC-P . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .67 RSF-1200 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .90 RTC-I-P . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .67 RTC-P . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .67 SGR . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .53, 55 SL-4 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .82 SL-8 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .82 UMC-2 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .56, 70 UMC-3 . . . . . . . . . . . . . . . . . . . . . . . . . .54, 56, 70 UMC-4 . . . . . . . . . . . . . . . . . . . . . .51, 5254, 70 UMC-5 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .70 UMC-5-2 . . . . . . . . . . . . . . . . . .55, 5758, 62, 70 UMP-1724 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .70
Indices
744
Easy Ordering Terms and Conditions
Easy Ordering Terms and Conditions

Pricing
Prices in this catalog are for reference only and are subject to change without notice. The pricing list for pub. 5988-4785ENUS is in U.S. dollars and represents U.S. list pricing. The pricing list for pub. 5988-4785ENCA is in Canadian dollars. Prices are effective January 1, 2002. Please check with your Agilent Customer Service Representative, sales office, local authorized distributor, or the Agilent website for current prices when placing your order.
Freight
A shipping and handling fee will be added to your order. Special shipping (e.g., overnight in the U.S.) is available in most regions at an additional cost. Shipping and handling fees are waived for purchases over $2,000 U.S.
Discounts
Agilent Technologies offers quantity discounts on a variety of individual products. In the U.S. and Canada, ask your Customer Service Representative about quantity discounts when you call in your order (1-800-227-9770). The entire quantity must be shipped at one time to one address to qualify for the discount. Order quantities may be limited on certain products. Outside the U.S. and Canada, contact your local Agilent sales office or authorized distributor. Dont forget to check our website for special offers, promotions, and discounts.
Return Policy: Satisfaction Guaranteed

If you are not satisfied with your Agilent consumables and supplies product within the first 60 days, you may return your purchase in its original condition for a full refund or credit. All items returned due to customer error must be new, unused, and in resell condition. Software must be unopened. A return policy statement is included in every Agilent shipment, and posted under Product Information on the website. Please phone for a Return Authorization form and return instructions at 1-800-227-9770, option 1, then 2, then 3. If your Agilent product was purchased from a distributor, please contact the distributor.
Shipping Damages
If items are damaged in transit, please follow the instructions below: 1. If a shipment is visibly damaged on arrival, do not accept it until the person making the delivery has endorsed the bill of lading with a statement for the extent of the damage. 2. If any damage is found after unpacking, retain all cartons and inner packaging at the shipment location and immediately request that the carrier arrange for an inspection. 3. Notify the Agilent Customer Contact Center at 1-800-227-9770 about the damaged shipment so we can make the appropriate sales adjustment and/or provide you with return instructions. Please provide us with the sales order number, product number, and quantity damaged.
745
Easy Ordering Terms and Conditions/Custom Column Orders
4. If instructed to return the damaged products to Agilent, please include copies of the bill of lading (with the carrier's statement of damage), return authorization number on packing material, and the inspection report.
Warranties
All Agilent Technologies products in this catalog are designed and manufactured to stringent standards under the Agilent quality system registered to ISO 9001. At Agilent, we back every product with a 90-day warranty and a money-back guarantee. If Agilent receives notice of defects during the warranty period, Agilent shall, at its option, either repair or replace products which prove to be defective. If Agilent is unable, within a reasonable time, to repair or replace any product to a condition as warranted, the buyer shall be entitled to a refund of the purchase price upon return of the product to Agilent. The warranty period for each product begins on the day of shipment. This warranty shall not apply to any defect, failure, or damage caused by improper use or improper or inadequate maintenance or care. This warranty is exclusive and no other warranty, whether written or oral, is expressed or implied. Agilent specifically disclaims the implied warranties of merchantability and fitness for particular purposes. The remedies provided herein are the buyers sole and exclusive remedies. In no event shall Agilent be liable for direct, indirect, special, incidental, or consequential damages (including loss of profits) whether based on contract, tort, or any other legal theory.
Fast Delivery
Agilent Technologies specializes in FAST DELIVERY. Over 97% of all orders for the U.S., Asia Pacific, and Europe are shipped from stock and we are working hard to achieve FAST DELIVERY for every order. In the U.S., if you call before 2 PM EST, we will ship your order that day. You can also ask for overnight EXPRESS DELIVERY before 6 PM EST and you will have your order in your hands the next day. Scheduled deliveries can be arranged upon request.
Taxes
Agilent is required to collect all state and local sales taxes on an order unless the buyers tax exempt certificate is on file with Agilent Technologies. If your certificate is not on file, please be prepared to provide a copy of your exemption certificate when phoning your order.
Custom Column Orders

Do you have special (columns not available as standard part numbers) LC or GC column requirements? Just let us know. Both Agilent GC capillary custom columns and Agilent LC ZORBAX custom columns for special applications are available upon request. If you are configuring GC capillary columns, use the form found on page 4. Close isnt good enough. Optimize your chromatography when you use PerfectFit affordable, quality, Agilent consumables and accessories with your Agilent instruments.
746
Ordering Information Custom GC Column Specification and Order Form

For best results, please photocopy this page, fill in the required information, and mail, fax, or email to Agilent Technologies. For all your custom capillary columns ordered, reference part number 100-2000. Agilent Technologies 2850 Centerville Road Wilmington, DE 19808 Attn: Customer Service Fax to: 302-633-8901 Email to: custom_columns@agilent.com (for U.S., Canada, and Puerto Rico) All other countries, please contact your local Agilent sales office or distributor.
If you require assistance, please contact 1-800-227-9770, press option 4, column technical support, then press option 1, GC phase application.
Type I
Type of Phase Length (m)
Internal Diameter (mm)
Film Thickness (m)
5" Cage or Standard Cage
Type II Duraguard
Column Part Number
or or
Fill in Parameters as Type I

Transfer Line Back End Length (m)
Guard Front End Length (m)
Type III Guard Column or Transfer Line

Leak Free U
Column Part Number
or or

Transfer Line Back End Note: Choose Guard Column or Transfer Line Internal Diameter (mm)
Guard Front End
Internal Diameter (mm)
Length (m)
Length (m)
Type IV Dual Column

Column #1
Column Part Number
or or
Column #2
Column Part Number

Guard Column Length (m) Internal Diameter (mm)
Leak Free Y
747
Custom Column Orders/Easy Ways to Order
Custom Column Ordering Procedure for ZORBAX HPLC Columns

HPLC columns not listed can easily be ordered by the following procedure: Request a Special Products Quotation (SPQ) using product number 899999-999. Indicate for this part number Column dimensions (example: 4.6 x 50 mm) Bonded phase type (example: StableBond C3) Particle size (example: 7 micron) Agilent Technologies 2850 Centerville Road Wilmington, DE 19808 Attn: Customer Service Fax to: 302-633-8901 Email to: custom_columns@agilent.com (for U.S., Canada, and Puerto Rico) All other countries, please contact your local Agilent sales office or distributor.
You will receive a quote from your Customer Service Representative within 1 to 2 business days. Delivery for your custom column is usually 3 weeks or less from the time your order is placed.
Easy Ways to Order

1. Phone us! In the U.S. and Canada, call 1-800-227-9770 or call your local authorized Agilent Technologies sales office or distributor. Our customer service representatives are waiting for your call Monday through Friday, from 8 AM to 8 PM EST. 2. Fax us! In the U.S. fax your order to: 302-633-8901. 3. Email us! In the U.S. and Canada email your order to us at cag_sales-na@agilent.com.
4. Order online at www.agilent.com/chem. Full ecommerce capabilities are available in the U.S. and Canada. Find everything you need online when you need it: pricing, availability, online ordering, order tracking, and more. Our online services are expanding every day. So register on our website today and sign up for our monthly email newsletter to keep up with the new features as they are added to our #1 rated website. We provide services to help you be more efficient every day:
Online ordering, including instruments and software Product availability Customer-specific product pricing Order status and tracking Order history Save and retrieve quotes Product and industry information
Technical and application information An extensive online literature library Parts identification diagrams Technical troubleshooting tools GC and LC reference chromatogram libraries e-Seminars
748
Easy Ways to Order Purchase Orders and Credit Cards
Order using a purchase order or credit card* . Credit cards currently accepted in the U.S. include: VISA MasterCard American Express
Opening a Charge Account

To open a charge account, give your companys billing and shipping address and a purchase order number to your Agilent Customer Service Representative or your local Agilent sales office. An account number will be assigned to you for charging your purchases. Payment terms are net 30 days from the invoice date. All orders are subject to credit approval.
Shipping and Handling

A nominal shipping and handling fee will be added to your order. Special shipping and handling is available in most regions at an additional cost.
Price Quotes
Just ask for a price quote and we will be happy to supply it over the phone, email, or via fax if you need a written quote.
* Not applicable in all countries. Credit card acceptance may vary with distributors. A minimum order of $20 is required.
749
Agilent Technologies Order Form

Order Date Purchase Order or Credit Card Number & Expiration Taxable Y or N?
Agilent Technologies
Yes No
Name: Title: Phone: Company:

Shipping Address
If No, please provide Certificate #
Fax: Email:
Billing Address
Company: Street: Room/Bldg/Dept: City: State/Province/Country: Zip/Postal Code: Deliver to:

Part Number Description Quantity Price Total Cost
Special Instructions:
Subtotal: Tax: Total:
For Assistance: Please call the Agilent Technologies Customer Contact Center at 1-800-227-9770 U.S. and Canada Email Node: cag_sales-na@agilent.com Fax Number: 302-633-8901 U.S. and Canada Note: 1. All pricing, tax, discount, and availability information is subject to verification by Agilent Technologies. 2. Shipping and handling is free for orders over $2,000 U.S.
750

Yes No

Shipping Address
Fax: Email:
Billing Address

751

Yes No

Shipping Address
Fax: Email:
Billing Address

752

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Table of Contents

Sales, Service, and Support

Featured Products General Chromatography

Information in this document is subject to change without notice.

Technical Support Information Technical Support at Work for You

Sales, Service and Support

Instrument Repair Parts Questions

Training/Services Information Chemical Analysis Training Programs

Service Bundles Meet Specific Needs for Less Cost

Services Information Configure Your Own Support Package

Sales, Service and Support

Off-Site Instrument Repair*

Maintenance Preventive Maintenance*

Mass Spectrometer Ion Source Cleaning*

Qualification Operational Qualification/ Performance Verification*

Telephone Support and Updates for Software

Agilent Offices Worldwide

Sales, Service and Support

Agilent Offices Worldwide

Sales, Service and Support

And it is growing every day!

Featured Products Demonstrating the Value of Deep Knowledge

Zorbax SPE Products

2002 Featured Products

Screw caps with Integrated Septa

Electronic Crimper and Decapper

Making Your Chromatography Lab More Productive

Vials, Caps and Septa

TIGHTLY CONTROLLED DIMENSIONS 12 mm

40 % LARGER OPENING THAN THE STANDARD MOUTH VIAL

OPTIONAL WRITE-ON PATCH WITH GRADUATIONS

Wide Opening 2 mL Vial

FLAT BOTTOM FOR INSERT STABILITY

Caps and Septa

Septum Selection Guide

Chlorinated solvents, aromatics, hydrocarbons, carbon disulfide

Agilent Vial Identification Chart and Actual Fill Volumes

51813375 Wide Opening Snap Top Vials

Inserts for 2 mL,12x32 mm Wide Opening Vials 100 L 100 L 250 L

Narrow Opening Screw Top Vials

51834428 Headspace Crimp Top VialsFlat and Rounded Bottom

Vials for Agilent LC Instruments

Agilent Screw Top Vials, Caps, Septa and Kits

Screw Top Vials

P/N 5182-0714, 5182-0715, 5182-0716

Deactivated Wide Opening Screw Top Glass Vials

Amber deactivated wide opening screw top, write-on spot 100/pk

Helpful Hint: Microvolume inserts are shown on pages 2728.

P/N 5183-2030, 5183-2073

Screw Top Micro-V Vial

Unit 100/pk 100/pk

Part No. 5184-3550 5184-3554

Micro-V vial, amber, screw top

Blue polypropylene cap, bonded pre-slit PTFE/silicone septa 100/pk

Polypropylene Screw Caps (no septa)

*Commonly referred to as red rubber

*Commonly referred to as red rubber

*Commonly referred to as red rubber

Agilent Crimp Top Vials, Caps and Kits

Unit 100/pk 100/pk 100/pk 1000/cs* 1000/cs* 1000/cs*

Part No. 5181-3375 5182-0543 5181-3376 5183-4491 5183-4492 5183-4493

Amber wide opening crimp top, write-on spot

1.5 mL Wide Opening Crimp Top High Recovery Glass Vials