QUALITY & FOOD SAFETY SOLUTIONS

Services and products for quality management:

DMD nutrient media for the microbiological detection of beverage-spoiling micro-organisms Analytical Services, accredited laboratory for product analysis Technical Services, specialist advice about quality management

WE BRING IDEAS TO LIFE.

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QUALITY & FOOD SAFETY SOLUTIONS

Premium beverages require a constantly high level of product and process safety. Standardised quality assurance of the products, combined with a continuous optimisation process, is a central component of modern quality management in accordance with DIN EN ISO 9001, IFS and BRC. As well as product analysis, it includes regular inspection of all process steps relevant to quality. The inuential factors are measured and analysed so that the ndings gained can be applied as quality assurance measures. Our customers recognize Dhler as a supplier of professional services and products (DMD) which are indispensable for quality assurance, especially in breweries and the non-alcoholic beverage industry.

DMD

ANALY TICAL & TECHNICAL SERVICES

Under the brand Dhler Microsafety Design (DMD) Dhler offers microbiologically selective nutrient media for almost all sectors of the beverage and brewing industry. They are easy to use and achieve fast, and above all reliable, results. Almost all are ready-to-use and can be deployed without additional costs or time-consuming treatment measures.

Dhler offers quality assurance services. These include analyses of your products in our laboratories and specialist advice from our technical eld service, always giving you an independent perspective in all questions relating to quality assurance in production.

Dhler Microsafety Design Simple Fast Safe

Dhler has been established as a supplier of microbiological nutrient media in the international beverage industry for more than 25 years. The media systems are recommended by ministries and institutes and even quoted in the standards authority benchmarks. This international acceptance is why our customers put their trust in the quality of the DMD products.

DMD CULTURES In the soft drink and juice industry for the detection of harmful moulds, yeast and bacteria p. 4 5

SSL-Broth for enrichment OFS-Agar TransFast System - TransFast-Broth for enrichment - TransFast-Gel

DMD benefits:

ready-to-use nutrient media simple application selective properties fast, safe and reliable results

In breweries for the detection of all relevant beer p. 6 7 and wine spoiling bacteria

NBB-Agar (NBB-A) NBB-Broth (NBB-B) NBB-Concentrate (NBB-C) NBB-Powder (NBB-P)

In close cooperation with professors from internationally renowned universities, institutes and laboratories, we develop products tailored to market demands and strive for innovative solutions which full your requirements.

In the juice industry for the detection of Alicyclobacillus

p. 8

BAT-Agar BAT-Broth Guaiacol detection kit

p. 8

For the detection of water-specic germs

Nutrient Agar in accordance with DEV LMC, concentrated lactose-pepton-broth in accordance with DEV

For the validation of aseptic lling lines

p. 9

AVM Aseptic Validation Medium

For the hygienic monitoring in the lling area

p. 9

NBB -B Enrichment Medium (NBB -B-AM)

Total view

p. 10

IN THE SOFT DRINK AND JUICE INDUSTRY FOR THE DETECTION OF HARMFUL MOULDS, YEAST AND BACTERIA

OFS-Agar, SSL-Broth, TransFast System for soft drinks

The Dhler nutrient media for non-alcoholic beverages allow the user to detect harmful micro-organisms in the entire beverage production process chain reliably and quickly. The cultures work extremely selectively. Only the bacteria responsible for the spoiling nd the ideal conditions to grow quickly. Almost all nutrient media are ready for use, and additional mixing or sterilisation is not necessary.

SSL-BROTH (pH 5.0 +/- 0.1) For the enrichment of beverage-spoiling bacteria APPLICATION

- for the enrichment of beverage-spoiling bacteria - can be used with ltered and non-ltered drinks - nutrient media for swab samples
BENEFITS

- inoculation of the sample after just 1-2 days - ready-made easy to use media - provides clear results on trace infections in combination with OFS agar

OFS-AGAR (pH 5.0 +/- 0.1) For the detection of yeast, moulds, acetic acid and lactic acid bacteria APPLICATION

- analysis of ready-to-drink beverages and rinsing water samples in the non-alcoholic beverage, dairy and pressing sectors as nutrient media from membrane lters - in the pour plate method - swabs from a wide range of samples
BENEFITS

- reliable results after 3 days - easy to use - very good quantitative and qualitative evaluation of results - some results can be evaluated macroscopically

TransFast System an evaluation within seconds

With TransFast, Dhler has succeeded in developing a completely new microbiological detection method in terms of how fast results are achieved. While classic media are poured into Petri dishes as agar, the carrier system in TransFast is a transparent gel. The beverage sample, which is usually enriched in advance, is placed with the gel into a transparent tube and incubated. Depending on the bacterial concentration, rst results can be seen after just 24 hours. Another innovation is an incubator with interior lighting. The direct illumination of the transparent samples allows any possible contamination to be assessed in a fraction of a second. Each beveragespoiling micro-organism, whether it be bacteria, yeast or mould, has a unique macroscopic appearance. There is no need for the time-consuming use of Petri dishes! Just turn on the light and you will be able to assess the overall condition of the samples immediately.

TRANSFAST -BROTH (pH 6.1 +/- 0.1) For the enrichment of beverage-spoiling bacteria APPLICATION

- can be used with compounds and ready-to-drink beverages - optimal in combination with TransFast-Gel
BENEFITS

- inoculation of the sample after just 1-2 days - high sample volume ensures statistical evaluation - ready-made easy to use media

TRANSFAST -GEL (pH 4.3 +/- 0.1) For the detection of yeast, moulds, acetic acid and lactic acid bacteria APPLICATION

- analysis of ready-to-drink beverages, beverage compounds, rinsing water enriched with TransFast-Broth - preparation of the sample in sterile TransFast-Tubes
BENEFITS

- evaluation of the samples in seconds due to uncomplicated screening - ready-made media replace time-consuming liquefying of the agar - rst results after 24 hours depending on the initial contamination - selective on beverage-spoiling micro-organisms

IN BREWERIES FOR THE DETECTION OF ALL RELEVANT BEER AND WINE SPOILING BACTERIA

NBB
Under the NBB brand, we offer nutrient media for the microbiological detection of beer-spoiling micro-organisms in breweries, which are characterised by being easy and quick to use and by their excellent selectivity. The microbiological complexity of beer demands high standards of an extremely sensitive culture for the detection of beer-spoiling bacteria. The result is a very selective and fast-working medium: NBB. Depending on the sample types in the brewery, NBB-Agar (NBB-A), NBB-

Broth (NBB-B), NBB-Concentrate (NBB-C) and NBB-B enrichment media (NBB-B-AM) are used. This means that the entire spectrum of samples which are taken in a brewery including turbid and yeastcontaining beers are covered. The precise ratio of special growth materials and inhibitors allows the development of an ideal medium with outstanding selective properties for harmful bacteria in beer. Brewers yeast and harmless secondary ora are largely inhibited. NBB is also suited for detecting pests in the wine industry.

NBB -BROTH (NBB -B) (pH 5.8 +/- 0.1) Analysis of yeast samples and cloudy, yeastcontaining beer samples APPLICATION

NBB -AGAR (NBB -A) (pH 5.8 +/- 0.1) Analysis of ltered beers APPLICATION

- as nutrient medium for membrane lters - in the pour plate method - swabs from a wide range of samples from the brewery (beer samples, water samples)
BENEFITS

- as nutrient medium for membrane lters - pre-enrichment of beer samples, even for methods in molecular biology (VIT, PCR)
BENEFITS

- agar works selectively, specically for beer - reliable results after just 5 days - fast evaluation using incorporated indicator colour - very good quantitative and qualitative evaluation of results - easy to use

- broth works selectively, specically for beer - reliable results after just 3 days - fast macroscopic evaluation using incorporated indicator colour - easy to use - also available in pre-lled test tubes for immediate use

NBB -CONCENTRATE (NBB -C) (pH 6.4 +/- 0.1) Analysis of cloudy and yeast-containing beer samples and yeast samples APPLICATION

Alternative to the ready-to use products NNB is also available as a powder. The essential growth materials, the inhibitors and the indicator colour are incorporated. For the making of the agar or the broth, own bier may be used to obtain a highly specic medium.

- as additive in the shelf life test samples - pre-enrichment of beer samples, even for methods in molecular biology (VIT, PCR)
BENEFITS NBB -POWDER (NBB -P) For the preparation of NBB-Agar and Broth with own beer APPLICATION

- adjustable selectivity of the culture using different concentrations and your own beer - records the full spectrum of beer-spoiling micro-organisms, from obligate to indicator bacteria, depending on the concentration used - reliable results after just 5 days

- use is analogous to NBB-B when the product is dissolved in demineralised water and decarbonated beer - in addition with agar-agar use is analogous to NBB-A when dissolved in demineralised water and decarbonated beer
BENEFITS

- adaptation of the proven NBB properties to the selectivity of your beers - small pack size makes it especially suitable for use with minimal cooling options

IN THE JUICE INDUSTRY FOR THE DETECTION OF ALICYCLOBACILLUS SPP.

FOR THE DETECTION OF WATER-SPECIFIC GERMS

BAT-Agar, BAT-Broth and Guaiacol detection kit

Alicyclobacillus can damage beverages by the formation of an off-avour (guaiacol, 2,6-dibromphenol, 2,6 dichlorophenol), although only A. acidoterrestris, A. acidiphilus and A. herbarius have shown the potential for forming off-avours up to now. The detection of Alicyclobacillus alone therefore does not provide conclusive information on the extent or risk posed of damaging a beverage. Instead, the risk potential for the formation of off-avours must be veried using the Guaiacol detection kit, an enzyme test.

Nutrient Agar, LMC-Concentrate in accordance with DEV

The requirements of the administrative bodies expect from beverage producers that process water meets the quality criterion of drinking water. This applies both for water processed in the product and for water used for rinsing and cleaning. Our nutrient agar in accordance with DEV allows the user to detect the total amount of colonies in water and in ready-to-drink beverages. With the LMC-concentrate, we are offering a product for the fast and uncomplicated detection of E.coli and coliform bacteria.

BAT-AGAR, BAT-BROTH (pH 4.0 +/- 0.1) AND GUAIACOL DETECTION KIT APPLICATION

AGAR NUTRIENT IN ACCORDANCE WITH DEV (pH 7.2 +/- 0.1) APPLICATION

- BAT-Broth is used for the enrichment of samples for the detection of Alicyclobacillus - BAT-Agar is used for the classic detection of Alicyclobacillus - the Guaiacol detection kit is an enzyme test to determine the potential for the formation of off avours through Alicyclobacillus
BENEFITS

- determination of the total viable count in water, food and beverage and rinsing water samples - nutrient medium for membrane lters - in the pour plate method - swabs from a wide range of samples
BENEFITS

- determination of Alicyclobacillus in accordance with IFU method No.12 - reliable, sensitive and economical method of analysis for Alicyclobacillus, even in complex products - reliable results after just 2 days - very easy to use

- nutrient medium for water analysis in accordance with the German uniformity process (DEV) and drinking water regulations - reliable results after just 1-2 days - simple to use

LMC-CONCENTRATE AS PER DEV (pH 7.2 +/- 0.1) APPLICATION

- detection of E.coli and coliform bacteria in water

BENEFITS

- samples are taken directly in the bottle pre-prepared with nutrient media and are also incubated in this - reliable results after just 2 days - fast evaluation using incorporated indicator colour

FOR THE VALIDATION OF ASEPTIC FILLING LINES

FOR THE HYGIENIC MONITORING IN THE FILLING AREA

AVM Aspetic Validation Medium

Aseptic lling lines must be tested for functionality after installation. It is recommended to use a standardised nutrient solution (AVM) which is lled aseptically under production conditions, just like a nished product. The lled bottles are incubated for 5 days and assessed visually checking the turbidity from contaminations. In addition, the AVM offers the option of checking the performance of the equipment components as a challenge test by selective inoculation with a germ suspension.

NBB -B Enrichment Medium

The risk of secondary contamination by beverage-spoiling microorganisms is highest in the lling and packaging area. It is therefore even more important to know the current hygiene status of the plant at all times. As part of an analysis of weak spots, critical areas in lling lines are dened and regularly sampled using streak swabs. The incorporated indicator colour in NBB-B-AM makes a positive result turn from red to yellow. If the percentage of positive samples remains above a safety threshold of 20% for a longer period, this indicates persistent biolms in which harmful organisms and other problematic bacteria will sooner or later be able to implant and adapt. A thorough general cleaning must be undertaken immediately or the risk of secondary infections grows due to the generation of aerosols.

AVM ASEPTIC VALIDATION MEDIUM APPLICATION

- validation of aseptic lling lines - requalication after maintenance or reconstruction work on the line
BENEFITS

NBB -B ENRICHMENT MEDIUM (NBB -B-AM) (pH 5.8 +/- 0.1) APPLICATION

- AVM is available as a ready-to-use solution, as a 5-fold concentrate or as a powder - exible pH-settings enables the application from acid to neutral range - reliable results after just 5 days - can detect trace or scattered contamination - simple visual assessment through formation of cloudiness in result - evaluation of the entire ll volume without the need for statistical multiplication - standardised tool as part of certication in accordance with standards such as DIN EN ISO 22000, BRC, IFS and the HACCP system

- as nutrient medium for swab samples from lling and production - for the detection of indicator bacteria for the prevention of secondary contamination - for all beverage-producing plants
BENEFITS

- the indicator bacteria at the source of any secondary infection are detected clearly and quickly, especially in the lling area - as macroscopic evaluation using incorporated indicator colour - easy to use - the hygiene standard of a plant can be permanently monitored and controlled using periodic analyses of weak spots - pre-enrichment of beer samples, even for methods in molecular biology (VIT, PCR)

MIKROBIOLOGICAL QUALITY CONTROL OF BEVERAGES, FOOD AND PRODUCTION FACILITIES WITH DMD
Test samples
Food and Beverages

Relevant microorganisms
Enumeration of the Total Plate Count (TPC)

Nutrient medium
Nutrient-A (Agar) as per DEV

Article no.
2.04726.782

Packaging
9 x 250 ml (glass bottle)

Water Potable water Spring water Table- and mineral water Well water Rinsing water Non-alcoholic beverages, Alcoholic mix beverages Ingredients Beverages pH < 4,5 Beer-, wine- and milk mix beverages Fruit juice concentrates Fruit bases Beverage emulsions Sugar Beverage powder or extracts Drinking and rinsing water Fruit juice concentrates Fruit bases Beverage emulsions Sugar Alcoholic Beverages, Ingredients Beer (ltered, unltered) Wine Mix beverages Beer wort Green beer Culture yeast Drinking and rinsing water

Water-specic faecal indicators E.Coli & Coliforms

LM-C (Lactose-Broth) as per DEV

2.04713.700

9 x 50 ml (glass bottle)

Beverage specic microorganisms

OFS-A (Agar) SSL-B (Broth)

2.04707.782 2.04712.782 2.04727.782 2.04731.782

9 x 250 ml (glass bottle) 9 x 250 ml (glass bottle) 9 x 250 ml (glass bottle) 9 x 250 ml (glass bottle)

Yeasts Lactic acid bacteria Acetic acid bacteria Moulds Heat resistant moulds (HRM) Spore forming bacteria

TransFast-B (Broth) TransFast-G (Gel)

Alicyclobacillus spp.

BAT-A (Agar) BAT-B (Broth) Guaiacol Detection Kit

2.04719.782 2.04717.782 2.04737.991

9 x 250 ml (glass bottle) 9 x 250 ml (glass bottle) incl. 40 tubes (box)

Beer- and wine specic microorganisms Lactobacilli Pediococci Megasphaera Pectinatus Culture yeast Wild yeast

NBB-A (Agar) NBB-B (Broth) NBB-B-Tubes (Broth) NBB-P (Powder) NBB-C (Concentrate) VLB S7S (Agar) VLB S7S (Broth) VLB Wort (Agar) VLB Lysine (Agar) VLB Copper Sulphate (Agar) VLB Standard (Agar)

2.04709.782 2.04710.782 2.04723.646 2.04716.462 2.04711.782 2.04785.782 2.04786.782 2.04787.244 2.04789.244 2.04790.244 2.04792.244 2.04705.050 2.04704.872 2.04755.521 2.04706.782

9 x 250 ml (glass bottle) 9 x 250 ml (glass bottle) 20 x 10ml (box) 300g (pouch) 9 x 250 ml (glass bottle) 9 x 250 ml (glass bottle) 9 x 250 ml (glass bottle) 9 x 250 ml (PC-bottle) 4 x 250 ml (PC-bottle) 4 x 250 ml (PC-bottle) 4 x 250 ml (PC-bottle) min. 3 to (tank car) 20 kg (basket) min. 3 to (container) 9 x 250 ml (glass bottle)

Production equipment and Filling lines Validation of aseptic lling lines

Beverage specic microorganisms

AVM (Ready-to-use) AVM -P (Powder)

Relevant microorganisms

AVM-C (Concentrate) NBB-BAM (Broth)

Weak spot analysis and step-by-step control

Indicator germs

Equipment for the laboratory

Sterile Swabs TransFast-Gel Tubes (75 ml) TransFast Incubation Light box

Article no.
2.04725.244 2.04730.001 4.40000.000

Packaging
100 (pouch) 100 (pouch)

Description
Sterile swabs on wood shaft, single packed Sterile disposable tubes, suitable for the usage of TransFast Gel Backlit incubator designed for the TransFast System

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Description
Complex agar for the enumeration of the total plate count, e.g. in water as per DEV (Deutsche Einheitsverfahren - German standard published by DIN)

pH (+/- 0,1)
7,2

Incubation
time [d]
2

temp. [C]
27 29

Reference
DIN Mineral- und Tafelwasser VO

Page
8

for water analysis 2 or 1 20 or 37

6-fold concentrated selective broth for the analysis of E.Coli and Coliforms in water samples as per DEV (Deutsches Einheitsverfahren - German standard published by DIN)

7,2

37

DIN, ISO, TrinkWV, Mineral- u. Tafelwasser VO

Selective agar for the quantitative detection and isolation of beverage spoilers used for membrane ltration or pour plate method Selective liquid enrichment medium as prestage for trace contamination Selective liquid enrichment medium as prestage for trace contamination with higher pH, even for samples with lower pH Selective and transparent liquid Gel for the qualitative detection of beverage spoilers

5,0 5,0 6,1 4,3

23 12 12 12

27 29 27 29 27 29 27 29

IFU (International Federation of Fruit Juice Producers)

Selective agar for the quantitative detection and isolation of Alicyclobacilllus spp. Selective liquid enrichment medium as prestage for trace contamination Enzymatic detection of guaiacol forming Alicyclobacilli for assessing the risk potential of off-avour forming Selective agar for the quantitative detection and isolation of beer or wine spoilers in ltered beer or wine used for membrane ltration or pour plate method, air sampling Selective liquid enrichment medium for the qualitative detection of beer and wine spoilers in culture yeast Tubes lled with NBB-Broth for the direct use Powder for the preparation of NBB-Agar and NBB-Broth Selective liquid enrichment medium, concentrated, for the detection of beer spoilers in unltered beer Selective agar for the quantitative detection of beer spoilers in ltered beer Selective liquid enrichment medium for the qualitative detection of beer spoilers in culture yeast Agar for isolating and determining colony count of yeast and moulds Agar for the detection of wild yeast Agar for the differentiation of culture and wild yeast Agar for the cultivation of fastidious bacteria (mesophilic and thermophilic) Nutrient medium for the validation of aseptic lling lines for beverages and for the validation of lling line components within challenge-testing

4,0 4,0

2 2 1

27 29 27 29 47

IFU (International Federation of Fruit Juice Producers) MEBAK (Mitteleuropische Brautechnische Analysenkommision)

5,8 5,8 5,8

57 35 35

27 29 27 29 27 29

67

6,4 5,5 5,6 5,6 4,8 5,8 7,5 3< pH <7,2

5 10 7 7 35 25 25 13 57

27 29 27 29 27 29 27 29 27 29 27 29 27 29 26 30 DIN, ISO 9

Liquid nutrient medium for the qualitative detection of indicator germs by swab analyses within the microbiological monitoring of production facilities

5,8

26 30

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ANALYTICAL SERVICES
Our accredited Analytical Services laboratory supports you with analyses of your ready-to-drink beverages - chemical/physical analysis (Brix, total acid, carbonation, pH) - microbiological analysis (aerobic and anaerobic beverage-spoiling microorgansims) Further analytical laboratories offer you very specic testing, such as avour and sugar analyses and the detection of specic pesticides. Make use of our comprehensive experience and benchmarks based on the large number of beverage samples which we receive from the international beverage industry for analysis. You will save on investment and resources in the establishment and maintenance of your own laboratories, space and staff. You can also have your own results conrmed periodically by our laboratory, giving security and creating trust. Testing procedures, such as specic and non-specic cultivation, are accredited compliant to DIN EN ISO/IEC 17025:2005 by Deutsche Akkreditierungsstelle Chemie GmbH in representation of the German Accreditation Council (DAkkS) for the subject areas of chemistry and biology.

TECHNICAL SERVICES
Take advantage of the many years of experience of our technical staff and save yourself time and money when completing projects at your own company. The DhlerGroups technical customer services / global technical support are an essential part of the integrated solution from the raw fruit ingredient to the ready-to-drink beverage. Our technical customer services support you on site in - sample llings - initial llings - recipe modications - mixing products With its high expertise, our technical customer services also performs analyses in microbiology and for hygiene management. This provides you with an independent evaluation of the hygiene status of your production equipment and lling lines.

DHLERGROUP Riedstrasse 7-9 64295 Darmstadt Germany Phone +49 6151 306-0 Fax +49 6151 306-278 www.doehler.com dmd@doehler.com

EDV-Nr.: 4.46102.282