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http://www.instructables.com/id/Photo-Bioreactor-for-Algae-amp-Aquaculture-Using/
step 1: Materials
As a college student, time can be purchased on campus laser cutters, so its one of the quickest means of producing components parts for any project, and was crucial in the construction of the photobioreactor. Besides having access or knowing where to find one of these amazing machines, youll need the following materials: Acrylic 1/16 inch Clear (7 x 36 )- youll need 2 of these 1/8 inch Smoke colored (15 x 36 ) 3/16 inch Clear (5.5 x 23 ) Acrylic adhesive I used Methylene Chloride from a plastic shop here in town, but JB Weld also makes glue that works on acrylic, youll have to look around for something that works well. Small aquarium air pump -I used a WhisperAir pump, but any pump should do since we're not dealing with very large amounts of water Aquarium Tubing 3-way airline splitters (Qty. 3) On/Off Airline valves (Qty. 4) Air Stones (Qty. 4) Aquarium Silicone Sealant - Hot glue could be substituted here Clear plastic Containers (Qty. 4) - Go out and dumpster dive for these, I found some great 1 liter SmartWater containers, so thats what I used when I designed my reactor. Algae I used bioluminescent algae, which can be obtained from the link below http://seafarms.com/html/products.html Aquarium Salt I used Instant Ocean, however this may be unnecessary depending on the type of algae you are using Water Conditioner -Easily available at meijer, Removes heavy metals and Chlorine from tap water to make sure you dont accidentally poison your starter cultures. BE SURE TO AVOID ONES THAT SAY ALGAE CONTROL THESE WILL KILL THE CULTURE! Algae Fertilizer
http://www.instructables.com/id/Photo-Bioreactor-for-Algae-amp-Aquaculture-Using/
This can be obtained here: https://3kserver7.com/~frank/secure/agora.cgi A Quick note on materials, the acrylic I used was what was available to me in the scrap bins here at school, so the design is based around that. If you plan on using alternate acrylic thicknesses, you will also need to edit the DXF Files I provide in the next steps to fit your material. Also, the tubing, valves, splitters, and water stones can be purchased in nice prepackaged sets at meijer for around 4 dollars.
http://www.instructables.com/id/Photo-Bioreactor-for-Algae-amp-Aquaculture-Using/
File Downloads
bio1.dxf (129 KB) [NOTE: When saving, if you see .tmp as the file ext, rename it to 'bio1.dxf']
smoke.dxf (70 KB) [NOTE: When saving, if you see .tmp as the file ext, rename it to 'smoke.dxf']
bio2.dxf (114 KB) [NOTE: When saving, if you see .tmp as the file ext, rename it to 'bio2.dxf']
bio3.dxf (75 KB) [NOTE: When saving, if you see .tmp as the file ext, rename it to 'bio3.dxf']
http://www.instructables.com/id/Photo-Bioreactor-for-Algae-amp-Aquaculture-Using/
Image Notes 1. Use a sering to apply the adheasive 2. join surfaces with tape, then apply adheasive to the joint
http://www.instructables.com/id/Photo-Bioreactor-for-Algae-amp-Aquaculture-Using/
Image Notes 1. Incoming airlin 2. Shut of Valves 3. first splitter 4. Second Splitter 5. Outgoing 12" hose to Culture Vessels 6. Looking at Interior base Panel A
Image Notes 1. Finished Base 2. Finished Support Structure with ribs attached
Image Notes 1. Looking at Interior Base Panel B 2. incoming air hose, connect to air pump
http://www.instructables.com/id/Photo-Bioreactor-for-Algae-amp-Aquaculture-Using/
http://www.instructables.com/id/Photo-Bioreactor-for-Algae-amp-Aquaculture-Using/
step 6: Congratulations!
Your almost thereNow with your Sweet Photo-BioReactor and your algae expertise, all you need is a lid and your ready to start your aquaculturing! Glue any remaining loose components in place. Then Glue the Lid Inserts onto one side of the lid. These will hold the Water Bottles together, while keeping out contaminates and stopping too much water from evaporating and the salinity from rising. Once you are sure that you dont have any leaks, you can insert the airpump and glue in the bottom panel of the reactor's base. Now step back and marvel at your Awesome, and Green in more ways than one, PhotoBioReactor! Not only are you reusing waste material (plastic water bottles) but this reactor will also actively scrub CO2 out or the air, while being a cool conversation piece when you have guests over. Imagine the possibilities if an inclined individual were to build in some sweet LED Grow lights... More instructables to come on what you can do with 4 litres of bioluminescent algae-
http://www.instructables.com/id/Photo-Bioreactor-for-Algae-amp-Aquaculture-Using/
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http://www.instructables.com/id/Photo-Bioreactor-for-Algae-amp-Aquaculture-Using/