Scribd Logo
Încărcați

Bine ați venit la Scribd!

  • 2nd Lab Depex Reviewer

    Încărcat de

    princelfangor
    66 vizualizări46 pagini
    This document summarizes laboratory exercises observing the development of frog and chick embryos through dissection and microscopy. For frogs, it describes the external morphology and internal structures of tadpoles at different developmental stages. For chicks, it provides details on the whole mounts and serial sections of embryos between 18-33 hours, noting features like the notochord, heart, gut, and somites at each level. The document compares the organogenesis and differentiation of tissues between the two species.

    Descriere originală:

    For Bio 30 Lab.

    Drepturi de autor

    © Attribution Non-Commercial (BY-NC)

    Formate disponibile

    PDF, TXT sau citiți online pe Scribd

    Vi se pare util acest document?

    Este necorespunzător acest conținut?

    Raportați acest document
    This document summarizes laboratory exercises observing the development of frog and chick embryos through dissection and microscopy. For frogs, it describes the external morphology and internal structures of tadpoles at different developmental stages. For chicks, it provides details on the whole mounts and serial sections of embryos between 18-33 hours, noting features like the notochord, heart, gut, and somites at each level. The document compares the organogenesis and differentiation of tissues between the two species.

    Drepturi de autor:

    Attribution Non-Commercial (BY-NC)
    Descărcați ca PDF, TXT sau citiți online pe Scribd
    Indicator pentru conținut neadecvat
    66 vizualizări46 pagini

    2nd Lab Depex Reviewer

    Încărcat de

    princelfangor
    This document summarizes laboratory exercises observing the development of frog and chick embryos through dissection and microscopy. For frogs, it describes the external morphology and internal structures of tadpoles at different developmental stages. For chicks, it provides details on the whole mounts and serial sections of embryos between 18-33 hours, noting features like the notochord, heart, gut, and somites at each level. The document compares the organogenesis and differentiation of tissues between the two species.

    Drepturi de autor:

    Attribution Non-Commercial (BY-NC)
    Descărcați ca PDF, TXT sau citiți online pe Scribd
    Indicator pentru conținut neadecvat
    din 46

    9 Jan 2014

    BIO LAB 2nd DepEx Reviewer


    I. EXERCISE 8: Longitudinal section of the external morphology of a tadpole

    organogenesis - differentiation of MESODERM

    Schumway's chart of frog development

    4-7 mm frog embryo : somites, gills, and tail

    10 mm tadpole: small fish with a prominent tail


    - gills
    - ventral suckers
    - horny jaws
    - lips and papillae
    - sense organs
    - myotomes

    II. EXERCISE 9: Serial Transverse section on the development of 10 mm tadpole


    A. Level of the Olfactory Placode

    internal nares = choanae

    external nares = olfactory pits

    lateral appendix = outgrowth near epidermis and external nares

    notochord: surrounded by FIBROUS SHEATH

    parachordal cartilage: basophilic nuclei of chondrocytes

    dorsal lip: 3 rows of teeth (3-D)

    venral lip: 4 rows of DECIDUOUS teeth (V-4)

    Layers of the brain wall


    -EPENDYMAL LAYER: supportive in function; w/ cilia To move CSF
    - MANTLE LAYER: becomes GRAY MATTER of CNS
    - MARGINAL LAYER: becomes WHITE MATTER of CNS

    B. Level of the Optic Cup

    mandibular muscles vs mandibular cartilage: muscles - fusiform (spindle-shaped) nuclei + greater


    staining affinity (darker)

    C. Level of the Heart <3

    Velar plate: prevents clogging of gill slits with ingested food

    gill rakers: prevent passage of solid particles through the gill slits; mechanical sifting of water

    internal gills: derived from branchial arches III, IV, and V

    GILL FILAMENTS

    D. Level of the Glottis

    E. Level of the lung buds

    nephrostome: lined by cilia; removal of larval waste products is accomplished by CILIARY MOTION

    spiracle: for egress of water

    F. Level of the mesonephros

    G. Level of the cloaca

    *************************************************************************
    22 Jan 2014
    I. 18-hour chick embryo whole mount

    *notochord (still a head process!)


    *HEAD ECTODERM
    II. SERIAL TRANSVERSE SECTION OF AN 18-HOUR CHICK EMBRYO
    A. LEVEL OF THE NOTOCHORD OR HEAD PROCESS

    *notochord/head process

    B. LEVEL OF THE HENSEN'S NODE

    C. LEVEL OF THE PRIMITIVE PIT

    D. LEVEL OF THE PRIMITIVE GROOVE

    *PRIMITIVE GUT
    *AREA PELLUCIDA
    *AREA OPACA

    III. WHOLE MOUNT OF A 24-HOUR CHICK EMBRYO

    *SUBCEPHALIC POCKET
    *MARGIN OF THE FOREGUT
    *NOTOCHORD

    IV. SERIAL TRANSVERSE SECTION OF A 24-HOUR CHICK EMBRYO


    A. LEVEL OF THE ANTERIOR NEUROPORE

    *FOREGUT

    *ORAL PLATE
    *SUBCEPHALIC POCKET

    B. LEVEL OF THE AMNIO-CARDIAC VESICLE

    *SPLANCHNOPLEURE

    C. LEVEL OF THE ANTERIOR INTESTINAL PORTAL

    D. LEVEL OF THE MIDGUT

    *CARDIAC PRIMORDIA= thickened splanchnic mesoderm

    E. LEVEL OF THE SOMITE

    F. LEVEL OF THE PRIMITIVE PIT

    *AREA OPACA VASCULOSA


    *AREA OPACA VITELLINA

    V. WHOLE MOUNT OF A 33-HOUR CHICK EMBRYO

    *PROSOCOEL
    *ANTERIOR NEUROPORE
    *NOTOCHORD
    *SINUS RHOMBOIDALIS
    *AUDITORY/OTIC VESICLE

    *AORTIC ARCHES
    *VITELLINE PLEXUS
    *SINUS TERMINALIS

    VI. SERIAL TRANSVERSE SECTION OF A 33-HOUR CHICK EMBRYO


    A. LEVEL OF THE PROSENCEPHALON

    *OPTIC VESICLES
    *OPTOCOEL
    *ECTODERM
    *MESENCHYME
    *BLOOD ISLANDS
    *HEAD FOLD
    *LATERAL BODY FOLD/ LATERAL LIMITING SULCUS

    B. MESENCEPHALON

    *FOREGUT
    *ORAL PLATE

    C. LEVEL OF THE HEART

    *OTIC PLACODE

    D. LEVEL OF THE MIDGUT

    *ANTERIOR INTESTINAL PORTAL


    *OMPHALOMESENTERIC VEINS

    E. LEVEL OF THE SOMITE

    *COELOM

    brought to you by: BOGS!


    ************ *********************END***********************************

    S-ar putea să vă placă și