247B Bioelectronics

January 10, 2013

Lecture Supplemental Materials Scale of Things, BioMolecular Structures, Bonding and Interactions, Water and Solvation, DNA/RNA and Proteins
Michael J. Heller, PhD
Departments Nanoengineering/Bioengineering University of California San Diego La Jolla, CA

The Scale of Things

Supplemental Cells and Biomoleclues

Cells and Biomoleclues

The Starting BioMolecules and Interactions

The Starting BioMolecules and Interactions

The Starting BioMolecules and Interactions

The Starting BioMolecules and Interactions

The Starting BioMolecules and Interactions

The Starting BioMolecules and Interactions

The Starting BioMolecules and Interactions

The Starting BioMolecules and Interactions

The Starting BioMolecules and Interactions

The Starting BioMolecules and Interactions

The Starting BioMolecules and Interactions

The Starting BioMolecules and Interactions

The Starting BioMolecules and Interactions

The Starting BioMolecules and Interactions

The Starting BioMolecules and Interactions

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Basics of DNA - A Key Biological Process DNA to RNA to Protein

Color Atlas of Biochem, Koolman J. et al, Thieme, 1996, pp.195

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Mol Bio Cell 4ed pp. 364

Basics of DNA - A Key Biological Process DNA to RNA to Protein

Intron Exon Sequence of Beta-Globin Gene
- Point Mutation !

Exon Intron

Hemoglobin Protein Structure

Lehninger pp. 196 & 189

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Basics of DNA Mutations and Genetic Diseases

Certain single base mismatches cause serious disease Sickle Cell Anemia and Thalassemia (numerous other genetic diseases and cancers)
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Lehninger pp. 220

Human Genome Project

Science, V291, Feb 16, 2001, pp. 1304-1351

Basics of DNA - Where is DNA Located in the Cells ?

Animal cells 5-30 microns

Nucleus

Plant cells 10-100 microns

Lehninger pp. 30 & 35

Basics of DNA - Cell - Nucleus - Chromosomes

Banding patterns of Human chromosomes

Mol Bio Cell 4ed pp. 199 & 202

Basics of DNA - Packaging of DNA in Chromosomes

Lehninger pp. 36

Fluorescent micrograph of cell in mitosis, green F spindle microtubules, red F centromeres, blue F condensed chromosomes. Mol Bio Cell 4ed pp. 556

Nucleotides and Nucleic Acids

Nucleotides and Nucleic Acids

Nucleotides and Nucleic Acids

Nucleotides and Nucleic Acids

Nucleotides and Nucleic Acids

Nucleotides and Nucleic Acids

Nucleotides and Nucleic Acids

The corresponding figure is on page 338 of your text

Nucleotides and Nucleic Acids

Ribosomal RNA More three dimensional type structures

Nucleotides and Nucleic Acids DNA Hybridization Process Denaturation and Annealing

FIGURE 8-29 DNA hybridization. Two DNA samples to be compared are completely denatured by heating. When the two solutions are mixed and slowly cooled, DNA strands of each sample associate with their normal complementary partner and anneal to form duplexes. If the two DNAs have significant sequence similarity, they also tend to form partial duplexes or hybrids with each other: the greater the sequence similarity between the two DNAs, the greater the number of hybrids formed. Hybrid formation can be measured in several ways. One of the DNAs is usually labeled with a radioactive isotope to simplify the measurements. 39

Nucleotides and Nucleic Acids Hybridzation - Heat denaturation, melting temperature (tm), other factors

Temperature, pH, size (# bps), G/C to A/T ratio, ionic strength, chem denaturants, detergents, chaotropics Stringency (high and low)

Basics of DNA - A Key Biological Process DNA to RNA to Protein

Mol Bio Cell 4ed pp. 315

Lehninger pp. 908

mheller@.ucsd.edu
Office: Atkinson Hall, Rm 2312 Phone: 858-822-5699