1 of 3 Biological Sciences 102 Winter, 2014 Course Syllabus

Staff Dr. Ken Hilt Lyudmyla Demyan Trisha Pfluger Michael Chiang (tutor) Nikki La (tutor) Wilson Ng (tutor) Kevin Nguyen (tutor)

Office Hours M 10 11 a.m. W 11 noon MW noon 1 p.m. F noon 2 p.m. Tu 5 7 p.m. W 2 3 p.m. W 8:30 10:30 a.m. W 3 4 p.m. M, Tu, Th 4 5 p.m. F 1 4 p.m.

Room E-mail Address 3033b SLB klhilt@ucdavis.edu 3033b SLB 3061 SLB ldemyan@ucdavis.edu 3061 SLB tmpfluger@ucdavis.edu 2061 SLB mkchiang@ucdavis.edu 2061 SLB 2061 SLB nila@ucdavis.edu 2064 SLB 2061 SLB wilng@ucdavis.edu 2061 SLB kvcnguyen@ucdavis.edu SLB = Sciences Lab Building

Administrative Support

Julie Evans, Hemang Patel, Maria Saldana-Seibert, and Valinda Stagner

Lectures

TuTh 12:10 p.m. 1:30 p.m., Rock Hall Audiotapes of each lecture are available on our Smartsite web page. Click Resources and then click Podcasts.

Texts Required Biochemical Calculations, 2nd ed., I. H. Segel, John Wiley & Sons, Inc., c1976 ISBN 0-471-77421-9

Optional Biochemistry, 5th ed., R. Garrett and C. Grisham, Brooks/Cole, c2013 ISBN-13: 978-1-133-10629-6 These and other texts are on reserve in Shields library.

Web Site Our web page is listed in SmartSite. All business items will be posted at this site: i.e. the course syllabus, assigned homework problems, and answer keys for homework problems and current exams.

2 of 3 Homework Homework is assigned, but not graded. These problems are previous exam questions. There are three homework assignments. Homework (HW) #1 covers material for the first midterm. HW #2 covers material for the second midterm. HW #3 covers half of the final exam (i.e. half of the final is equivalent to a third midterm; the other half of the final exam is cumulative).

Exams Exam First Midterm Second Midterm Final Date Thursday, Feb. 6 Thursday, Feb. 27 Friday, Mar. 21 Time 12:10 p.m. 1:30 p.m. 12:10 p.m. 1:30 p.m. 8:00 a.m. 10:00 a.m. Points 100 100 200 % of Course Grade 25 25 50

We will have an optional discussion section prior to each exam. Each exam tests your understanding of concepts and quantitative analyses. Equations and constants are furnished. However, you must know definitions. Bring a nonprogrammable, nongraphing calculator. Missed Midterms: the only excuse for missing a midterm is a written medical excuse from your personal physician or Cowell Student Health Center. For anything else, you will need to set up an appointment with me by the following Monday to show your proficiency in the material by having an oral exam. If you pass, I will then move the percentage of the midterm to the final.

How to do well in the course Focus on the lecture material and assigned homework. Share your notes and argue concepts with a fellow classmate. Go to office hours.

3 of 3 Lecture 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 Date Jan. 7 9 14 16 21 23 28 30 Feb. 4 6 11 13 18 20 25 27 Mar. 4 6 11 13 21 Tentative Lecture Schedule Topic Course introduction, biochemical components of cells, amino acids Amino acids properties of R-groups Weak acids/bases, net charge Weak bonds Oligopeptides; separation of amino acids via ion exchange chromatography Protein structure (1o and 2o) Protein structure (3o) myoglobin example Protein structure (4o) hemoglobin example Protein structure (4o) hemoglobin example First Midterm Protein folding Enzymes active sites, chymotrypsin example Enzymes assays, Michaelis-Menten equation Enzymes reversible inhibitors, Lineweaver-Burk plots Protein purification initial steps Second Midterm Protein purification affinity chromatography, IEF gels Protein purification native PAGE gels, SDS-PAGE gels Lipids classes, fatty acid components Membranes composition, fluidity, transport across; course summary Final Exam 8:00 a.m. 10:00 a.m. (50% covers material since the second midterm; the other 50% reexamines the material for the two midterms)

Suggested Reading (chapters) Lecture Segel 1-5 6-9 11 1, 2 2

Garrett and Grisham 1, 2, 4 6, 15 6, 31

Lecture 1214 15-18 19 20

Segel 4 2

Garrett and Grisham 13, 14, 15 5 8 9