1. You are starting a new rotation in a Drosophila lab.

While looking at some of the lab stocks to familiarize yourself with flies, you notice a female with a spotted body phenotype in a vial of wildtype flies. You decide you want to characterize this mutation further. First, you cross the fly to a male wildtype fly to see if the mutation is dominant or recessive. alf of the progeny flies from this cross are spotted, and half have wildtype pigmentation, so you conclude that the mutation is dominant, and the spotted flies are heterozygous at the mutant locus. 1a. Your ne!t step is to map the mutation to a chromosome. "iven the following strains, what crosses would you perform to show that the spotted body mutation maps to the # chromosome$ %nclude your e!pected results if the mutation does or does not map to the # chromosome. &train genotype Sp ' ( F)*'Y +',yDescription )utant strain heterozygous for the spotted body mutation )ales containing an # chromosome balancer with a dominant bar eye phenotype +. chromosome / marker that causes a dominant lobe0shaped eye phenotype, recessive lethal ,y-. chromosome / balancer that has a dominant curly0winged phenotype, recessive lethal )ale and female wildtype flies

('(, ('Y

1b. You do this cross and confirm that the mutation maps to the # chromosome. owever, you also notice something strange. During the crosses, you never found a spotted body male, only females. What are two possible e!planations for this$ 1c. You decide you want to map e!actly where the mutation lies on the # chromosome. 1o do so, you cross spotted body females with a true breeding strain containing recessive mutations b and c 2note. these are 3ust placeholders, % don4t know many # chromosome markers5. You take the spotted body females from this cross and cross them to b and c males. 1he phenotypes of the F/ progeny are listed in the table below. F/ 6henotype Wildtype &potted b mutant phenotype c mutant phenotype &potted b mutant &potted c mutant 7umber 898 //:; ;8 1;1 *< //

b mutant c mutant &potted b mutant c mutant 1otal

::=> 1;/ *;99

Draw a map of the 8 genes that includes the gene order and the distances between ad3acent genes. 1d. ?sing mosaic analysis, you would like to determine whether being homozygous mutant would be lethal for females. "iven the following reagents and strains, briefly e!plain how you would do this. What would you e!pect if the mutations were lethal$ %f they weren4t lethal$ "iven. 0 &train 1. heterozygous for spotted body and for a recessive marker on the # chromosome @white eyes$A. Blso contains an FC1 site on the # chromosome. 0 F+6 recombinase 1e. From part d, you find out that sp is recessive lethal, so females with two copies of the mutation also die. You would like to identify a temperature0sensitive allele of this gene in order to e!amine its effect in males at different stages of development. D!plain the crosses you would perform in order to do so given the following strains and a mutagen. &train genotype F)*'F)*, F)*'Y ('(, ('Y &p1 D' ( D Description )ale and female flies with chromosome 1 balancer @see 1a for descriptionA Wildtype male and female flies Female flies with original spotted body mutation and dominant EDF marker