Hemolytic Anemia Induced by Babesia gibsoni Infection in American Pit Bull Terrier- Case Report

Author : Crina Sorina GH RGH !" Aura Stefania PAR#I" Cristina-Andreea $IR%! T Scientific Coordinator: &rsolya S'RPATA(I " )ef*lucr* dr*Bo+dan S ,ASTR University of Agricultural Sciences and Veterinary Medicine, Faculty of Veterinary Medicine 400372, Calea Mn tur 3!", Clu#!$a%oca, &o'ania g(erg(el)crina*ya(oo+co'

ABSTRACT

Canine ,a,esios is a tic-!,orne %roto.oal disease, c(aracteri.ed ,y (e'olytic ane'ia and t(ro',ocyto%enia+ Until recently, only /a,esia canis 0as identified as t(e etiologic agent of canine ,a,esiosis in &o'ania+ 1n Marc( 2023 five canine ,lood sa'%les 0ere sent to our la,oratory+ All sa'%les ca'e fro' A'erican 3it /ull 4erriers 5A3/46 0it( various clinical sy'to%'s as anore7ia, fever, de%ression, e7ercise intolerance, %ale 'ucouse 'e',ranes and (e'aturia+ /a,esia gi,soni 8$A 0as detected ,y 3C&+Co'%lete ,lood count 0as %erfor'ed 0it( A,acus 9unior Vet auto'atic analy.er+ /lood s'ears 0ere stained using 8iff :uic- and ;ie'sa staining+ For reticulocyte count Cresyl /lue stain 0as used+ 4(ree of five ,lood sa'%les s(o0ed severe ane'ia and tro',ocyt(o%enia furt(er'ore t(e 'icrosco%ic e7a'ination s(o0ed s'all %leo'or(ic inclusions in red ,lood cells 'easuring 2!2+" <'+ 4(e increased nu',er of reticulocites indicate regenerative ane'ia+ Babesia gibsoni 8$A 0as detected ,y %oly'erase c(ain reaction 53C&6 assay in t(ese t(ree ,lood sa'%les+

( - .&R#S: A'erican 3it /ull 4erriers, /a,esia gi,soni, canine, 3oly'erase c(ain reaction assay,&o'ania R $ R /C S 2+ /ir-en(euer A9, M; =evy, >C Savary, &/ ;ager, ?/ /reitsc(0erdt, /a,esia gi,soni infections in dogs fro' $ort( Carolina, 9ournal of t(e A'erican Ani'al @os%ital Association, 2AAA, 3"B22"!22C

2+ Cardoso =+, Disasc(ar!Me-u.as D+, &odrigues F+4+, Costa A+, Mac(ado 9+, 8i.!=o%es 8+, /anet( ;, Canine ,a,esiosis in nort(ern 3ortugal and 'olecular c(aracteri.ation of vector!,orne co!infections, 3arasit Vectors, 2020, 3B27

Anemie hemolitic0 indus0 de Babesia gibsoni la American Pit Bull Terrieristudiu de ca1
Autor : Crina Sorina GH RGH !" Aura Stefania PAR#I" Cristina-Andreea $IR%! T Coordonator tinific: drd* &rsolya S'RPATA(I " )ef*lucr*dr* Bo+dan S ,ASTR Universitatea de tiine Agricole i Medicin Veterinar, Facultatea de Medicin Veterinar 400372, Calea Mn tur 3!", Clu#!$a%oca, &o'ania g(erg(el)crina*ya(oo+co' R 2%3AT /a,esio.a canin este o %roto.oo. trans'is %rin inter'ediul c%u elor, caracteri.at %rin ane'ie (e'olitic i tro',ocito%enie+ 3En de curEnd, doar Babesia canis a fost identificat ca agent etiologic al ,a,esio.ei canine Fn &o'Enia+ Gn 'artie 2023 cinci %ro,e de sEnge au fost tri'ise la la,oratorul de (e'atologie din cadrul 8isci%linei de Fi.io%atologie+ 4oate %ro,ele %roveneau de la A'erican 3it /ull 4errier 5A3/46, din trei regiuni geografice diferite din &o'Enia, cu diverse si'%to'e clinice ca anore7ie, fe,r, de%resie, intoleran la efort fi.ic, 'ucoase %alide i (e'aturie+ @e'oleucogra'a co'%let a fost efectuat cu anali.atorul auto'at A,acus 9unior Vet+ Frotiurile de sEnge au fost colorate folosind coloraiile 8ia :uic- i ;ie'sa+ 3entru nu'rarea reticulocitelor a fost folosit coloraia s%ecial cu Al,astru /riliant de Cresil+ 4rei din aceste cinci %ro,e %re.entau ane'ie i tro',ocito%enie sever, iar la e7a'enul 'icrosco%ic s!au identificat inclu.ii intraeritrocitare %oli'orfe, de di'ensiuni 'ici, 'surEnd 2!2+"<'+ $u'rul crescut de reticulocite indic ane'ie regenerativ+ 8iagnosticul a fost confir'at %rin anali.a 3C&, detectEnd A8$ de Babesia gibsoni Fn cele trei %ro,e+ C%,I/T CH I : Babesia gibsoni, cEine, rasa A'erican 3it /ull 4errier , 3C&, &o'Enia+ R $ R /C S 2+ /ir-en(euer A9, M; =evy, >C Savary, &/ ;ager, ?/ /reitsc(0erdt, /a,esia gi,soni infections in dogs fro' $ort( Carolina, 9ournal of t(e A'erican Ani'al @os%ital Association, 2AAA, 3"B22"!22C

2+ Cardoso =+, Disasc(ar!Me-u.as D+, &odrigues F+4+, Costa A+, Mac(ado 9+, 8i.!=o%es 8+, /anet( ;, Canine ,a,esiosis in nort(ern 3ortugal and 'olecular c(aracteri.ation of vector!,orne co!infections, 3arasit Vectors, 2020, 3B27