Subject: integrated science Class: 8F Topic: Healthy living Sub-topic: Biuret test for protein Objectives: To test for

the presence of protein in different food groups To observe and record changes that occurs during e peri!ent To identify "hich food contains proteins # variety of solutions to test: cornstarch $!i ed "ith "ater% !il& chic&en broth "heat flour $!i ed "ith "ater% orange juice "ater vinegar uns"eetened po"dered le!onade $!i ed "ith "ater% Content: 'roteins are i!portant for !any processes "ithin living things( They contribute to the overall structure of a cell such as !uscle cells) to binding to specific !olecules such as the protein he!oglobin that binds to o ygen) and to cataly*ing che!ical reactions in the cell through proteins &no"n as en*y!es( 'roteins are co!posed of building bloc&s &no"n as a!ino acids( There are +, total a!ino acids( 'roteins are long chains of a!ino acids( The length of the chain and the precise se-uence of the a!ino acids in the chain deter!ines "hat the protein can do( The Biuret test is a si!ple test for the presence of proteins( Biuret solution is a blue solution that "ill react "ith proteins to !a&e a pin&-purple color( .( +( 0( /( Set up test tube rac&s "ith / test tubes per rac& Set out test solutions and bea&ers Set out eyedroppers Set out labeling tape and per!anent !ar&ers

Lesson Plan .( The class "ill begin "ith a discussion of food and the food groups( 1ach of the !ajor organic !olecules can be correlated to different classes of foods $protein 2 !eat and beans group) si!ple sugars $glucose and fructose% 2 s"eets and fruit groups) co!ple carbohydrates $starch% 2 grains group) lipids 2 fats group%( #llo" this discussion to lead into the idea that all food ite!s are !ade up of building bloc& organic !olecules( Food

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ite!s "ere all once alive( Therefore) all living things are !ade up of these sa!e building bloc&s that our food is !ade of( 'ass out the student lab handouts( 3escribe the activity to your students( 1ach student "ill test solutions at 0 different stations( Sho" students any special procedures) such as ho" to prepare the hot "ater bath( #ns"er any -uestions( #llo" students 4 !inutes to gather their !aterials and then .,-.4 !inutes at each test station( Help groups that are having difficulty( 5hen all testing is co!plete and tea!s have cleaned up) a !aster table "ill be created on the board li&e the one belo" to collect all the tea!s results for all the different tests(

'rotein test Chic&en broth 5heat flour Orange juice 5ater 6ubbing alcohol 3iluted dish soap 7inegar Fish tan& "ater or pond "ater 8ns"eetened po"dered le!onade

Assessment .( Collect student noteboo&s "ith data tables and conclusion -uestions(

Discussion: Biuret reagent is a mixture of sodium .. and copper .. . It is the copper sulfate that gives the Biuret reagent its blue colour. Proteins are essential to us because we use them for gr of new bones skin etc. and the re.. of damaged tissue. Your body also uses proteins to control the chemical reactions in our body and can also be used as an energy source. Some common examples of protein foods include mi, f, m.. eggs and seeds.