Biotechniques Participant

BIOTECHNIQUES
Mr. Thaddeus Owen D. Ayuste

Faculty of Science, Technology and
Mathematics
College of Teacher Development
Philippine Normal University
1
1. A teacher will show actual feeding habits of
some animals to his students. Which will be the
best visual aid?
A. Microtechniques
B. Taxidermy
C. Herbarium
D. Aquarium

2
From Part II: Analyzing Test Items-Question # 4
1. A teacher will show actual feeding habits of some
animals to his students. Which will be the best visual
aid?
3
Micro
techniques
Taxidermy
Herbarium
Aquarium
2. Luis is ready to cut specimens thinly for slide
preparation. What instrument does he need?
A. Stain
B. Incubator
C. Microtome
D. Fine needles

4
2. Luis is ready to cut specimens thinly for slide
preparation. What instrument does he need?
5
Sectioning
Stain
Incubator
Microtome
Fine
needles
3. If an incubator is to be improvised, which will
provide the source of heat?
A. Bulb
B. Lightning
C. Porcelain
D. Thermometer

6
3. If an incubator is to be improvised, which will
provide the source of heat?
7
Bulb
Lightning
Porcelain
Thermo-
meter
4. A teacher will use herbarium in teaching. Which
topic is suitable for herbarium?
A. Identifying the name of the plant
B. Osmosis of water to leaves
C. Showing photosynthesis
D. Respiration in plants

8
4. A teacher will use herbarium in teaching. Which
topic is suitable for herbarium?
9
Preserved plants; dead plants
Identifying the
name of the
plant
Osmosis of
water to leaves
Showing
photosynthesis
Respiration
in plants
5. A teacher will use taxidermy in teaching. Which
topic is suitable for taxidermy?
A. Skin covering
B. Feeding habits
C. Skeletal system
D. Muscular system

10
5. A teacher will use taxidermy in teaching. Which
topic is suitable for taxidermy?
11
Integument is given utmost importance
Skin is preserved
Good for aquarium
Skeleton is removed
in taxidermy
Muscles are removed
in taxidermy
Skin covering
Feeding habits
Skeletal system
Muscular system
6. In Microtechniques, what comes last?
A. Drying, cleaning, and labeling
B. Deceration, staining and mounting
C. Washing, dehydration and clearing
D. Infiltration, embedding and sectioning

12
6. In Microtechniques, what comes last?
13
Labeling of slides
With Labeling
No Labeling
No Labeling
No Labeling
Drying, cleaning, and labeling
Deceration, staining
and mounting
Washing, dehydration
and clearing
Infiltration, embedding
and sectioning
7. In the absence of a microtome, free hand section
can be used. What cutting material is
recommended?
A. Probe
B. Scalpel
C. Forceps
D. Microscope

14
7. In the absence of a microtome, free hand section can
be used. What cutting material is recommended?
15
Cutting
Probe
Scalpel
Forceps
Microscope
8. Juan is a taxonomist. He correctly gives scientific
names of plant specimens collected in the field.
Which will greatly help Juan in naming?
A. Microtechniques
B. Herbarium
C. Aquarium
D. Terrarium

16
From Part III: Enhancing Test taking Skills-Question # 9
8. Juan is a taxonomist. He correctly gives scientific
names of plant specimens collected in the field. Which
will greatly help Juan in naming?
17
Classification; naming
Micro-
techniques
Herbarium
Aquarium
Terrarium
9. A teacher wants to show blood samples on a
slide. Which method will she use?
A. Squashing
B. Smearing
C. Teasing
D. Paraffin

18
9. A teacher wants to show blood samples on a slide.
Which method will she use?
19
Liquid
Squashing
Smearing
Teasing
Paraffin
10. The following are steps in microtechinques, a
procedure of making slides. Which comes first?
A. Anesthetization
B. Sectioning
C. Dissection
D. Mounting
20
10. The following are steps in microtechinques, a
procedure of making slides. Which comes first?
21
Specimens must feel the least pain
Anesthe-
tization
Sectioning
Dissection
Mounting
11. Which chemical is usually used in preserving
specimen in microtechniques?
A. Formalin
B. Xylene
C. Water
D. Stain

22
11. Which chemical is usually used in preserving
23
Prevents further damage to the specimen; fixation
Preservation
Clearing
Washing; solvent
Coloration
Formalin
Xylene
Water
Stain
12. A teacher wants to show the movement of
animals in his lesson. What should he use?
A. Herbarium and taxidermy
B. Terrarium and herbarium
C. Terrarium and taxidermy
D. Aquarium and terrarium

24
12. A teacher wants to show the movement of animals
in his lesson. What should he use?
25
Proof of being alive
Herbarium and taxidermy
Terrarium and herbarium
Terrarium and taxidermy
Aquarium and terrarium
X

X
X

X
13. Which instrument can substitute a microtome?
A. Pin
B. Scalpel
C. Incubator
D. Thermometer

26
13. Which instrument can substitute a microtome?
27
For cuting; for sectioning
Pin
Scalpel
Incubator
Thermo-
meter
14. A teacher asks students to prepare pollen
grains on slides. Which skill is needed?
A. Setting up of aquarium
B. Preparing taxidermy
C. Making herbarium
D. Microtechiques

28
14. A teacher asks students to prepare pollen grains on
slides. Which skill is needed?
29
Microscopic
Setting up of
aquarium
Preparing
taxidermy
Making
herbarium
Microtechiques
15. In culturing bacteria, agar is used. Which is
NOT a reason for this use?
A. liquefies at 100
0
C and remains liquid until about
40
0
C
B. may be used in petri dish or test tube
C. hard to prepare in the laboratory room
D. few microbes can degrade it

30
15. In culturing bacteria, agar is used. Which is NOT a
reason for this use?
31
Growing bacteria
True for agar
True for agar
False for agar
True for agar
liquefies at 100
0
C and remains
liquid until about 40
0
C
may be used in petri dish
or test tube
hard to prepare in the
laboratory room
few microbes can degrade it
16. Hay infusion is used for protist study. It has to
be left for a few days before using. Why?
A. Prevents bacteria from colonizing
B. Releases energy from the protists
C. Allows growth of protist population
D. Ensures sunlight kills the unwanted organisms

32
16. Hay infusion is used for protist study. It has to be
left for a few days before using. Why?
33
No protists yet in the initial setup of hay infusion
Bacteria are posssible food of
the protist
Hay is the source of food for
bacteria
Protist will exist once food is
available for them
Sunlight will not kill the
organisms
Prevents bacteria from
colonizing
Releases energy from
the protists
Allows growth of
protist population
Ensures sunlight kills the
unwanted organisms
17. Which water source can be a direct substitute
for hay infusion?
A. Pond
B. Rain
C. Soil
D. Tap

34
17. Which water source can be a direct substitute for
hay infusion?
35
Liquid
Liquid; usually with prostists
Liquid; no time for the protist
to grow
Solid
Liquid; usually treated with
chlorine
Pond
Rain
Soil
Tap
18. Which is a good technique to use in preparing
blood specimens?
A. Squashing
B. Smearing
C. Grinding
D. Teasing

36
18. Which is a good technique to use in preparing
blood specimens?
37
Liquid
Squashing
Smearing
Grinding
Teasing
19. Which chemical is usually used in dehydration
of specimen in microtechniques?
A. Formalin
B. Alcohol
C. Xylene
D. Water
38
Additional Question
19. Which chemical is usually used in dehydration of
39
Removal of water
Additional Question
Formalin
Alcohol
Xylene
Water
For preservation For clearing
For washing
For dehydration
20. An entire ant is chosen to be the specimen for
mounting. What should be included in the label of
the slide?
A. wm
B. cs
C. eg
D. ls

40
Additional Question
20. An entire ant is chosen to be the specimen for
mounting. What should be included in the label of the
slide?
41
Whole
Additional Question
wm
cs
eg
ls
Whole mount
Cross section
Example
Longitudinal section
God bless in your LET!
42

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Mr. Thaddeus Owen D. Ayuste

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