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lvarez,
J.M., Acebes, J.L., and Hayashi, T. (2007). Increase in XET ac-
tivity in bean (Phaseolus vulgaris L.) cells habituated to di-
chlobenil. Planta 226, 765771.
Barik, S. (1997). Mutagenesis and gene fusion by megaprimer
PCR. In Methods in Molecular Biology, vol. 67. PCR cloning
protocols: from molecular cloning to genetic engineering. B.A.
White, ed. (Humana Press Inc., Totowa, NJ), pp. 173182.
Chen, J.R., Lu , J.J., and Wang, H.F. (2008). Rapid and efcient
gene splicing using megaprimer-based protocol. Mol Bio-
technol 40, 224230.
Chen, J.R., Lu , J.J., Wang, T.X., Chen, S.Y., and Wang, H.F.
(2009). Activation of a DRE-binding transcription factor from
Medicago truncatula by deleting a Ser=Thr-rich region. In Vitro
Cell Dev Biol Plant 45, 111.
Chuang, C., and Meyerowitz, E. (2000). Specic and heritable
genetic interference by double-stranded RNA in Arabidopsis
thaliana. Proc Natl Acad Sci USA 97, 49854990.
Coljee, V.W., Murray, H.L., Donahue, W.F., and Jarrell, K.A.
(2000). Seamless gene engineering using RNA- and DNA-
overhang cloning. Nat Biotechnol 18, 789791.
Dafny-Yelin, M., Chung, S.M., Frankman, E.L., and Tzra, T.
(2007). pSAT RNA interference vectors: a modular series for
multiple gene down-regulation in plants. Plant Physiol 145,
12721281.
Fang, G., Weiser, B., Visosky, A., Moran, T., and Burger, H. (1999).
PCR-mediated recombination: a general method applied to
construct chimeric infectious molecular clones of plasma-
derived HIV-1 RNA. Nat Med 5, 239242.
Hamilton, A., Voinet, O., Chappell, L., and Baulcombe, D.
(2002). Two classes of short interfering RNA in RNA silencing.
EMBO J 21, 46714679.
Heckman, K.L., and Pease, L.R. (2007). Gene splicing and
mutagenesis by PCR-driven overlap extension. Nat Protoc 2,
924932.
Hirai, S., Oka, S.I., Adachi, E., and Kodama, H. (2007). The
effects of spacer sequences on silencing efciency of plant
RNAi vectors. Plant Cell Rep 26, 651659.
Johansen, L.K., and Carrington, J.C. (2001). Silencing on the spot
induction and suppression of RNA silencing in the Agro-
bacterium-mediated transient expression system. Plant Physiol
126, 930938.
Johansson, P., Brumer, H., Baumann, M.J., Kallas, A.M., Hen-
riksson, H., Denman, S.E., Teeri, T.T., and Jones, T.A. (2004).
Crystal structures of a poplar xyloglucan endotransglyco-
sylase reveal details of transglycosylation acceptor binding.
Plant Cell 16, 874886.
Kammann, M., Laufs, J., Schell, J., and Gronenborn, B. (1989).
Rapid insertional mutagenesis of DNA by polymerase chain
reaction (PCR). Nucleic Acids Res 17, 5404.
Ke, S.H., and Madison, E.L. (1997). Rapid and efcient site-
directed mutagenesis by single-tube megaprimer PCR
method. Nucleic Acids Res 25, 33713372.
Kumagai, H., and Kouchi, H. (2003). Gene silencing by expres-
sion of hairpin RNA in Lotus japonicus roots and root nodules.
Mol Plant Microbe Interact 16, 663668.
Kuwayama, H., Obara, S., Morio, T., Katoh, M., Urushihara, H.,
and Tanaka, Y. (2002). PCR-mediated generation of a gene
disruption construct without the use of DNA ligase and plas-
mid vectors. Nucleic Acids Res 30, e2.
Limpens, E., Ramos, J., Franken, C., Raz, V., Compaan, B.,
Franssen, H., Bisseling, T., and Geurts, R. (2004). RNA inter-
ference in Agrobacterium rhizogenes-transformed roots of Ara-
bidopsis and Medicago truncatula. J Exp Bot 55, 983992.
Miki, D., and Shimamoto, K. (2004). Simple RNAi vectors for
stable and transient suppression of gene function in rice. Plant
Cell Physiol 45, 490495.
Picard, V., Ersdal-Badju, E., Lu, A., and Bock, S.C. (1994). A
rapid efcient one-tube PCR-based mutagenesis technique
using Pfu DNA polymerase. Nucleic Acids Res 22, 2587
2591.
Sandhu, G.S., Aleff, R.A., and Kline, B.C. (1992). Dual asymmetric
PCR: one-step construction of synthetic genes. Biotechniques
12, 1416.
Sarkar, G., and Sommer, S.S. (1990). The megaprimer method
of site-directed mutagenesis. Biotechniques 8, 404407.
Schweizer, P., Pokorny, J., Schulze-Lefert, P., and Dudler, R.
(2000). Double-stranded RNA interferes with gene function at
the single-cell level in cereals. Plant J 24, 895903.
Smith, N.A., Singh, S.P., Wang, M.B., Stoutjesdijk, P.A., Green,
A.G., and Waterhouse, P.M. (2000). Total silencing by intron-
spliced hairpin RNAs. Nature 407, 319320.
So derberg, M., and Lang, M.A. (2006). Megaprimer-based
methodology for deletion of a large fragment within a repeti-
tive polypyrimidine-rich DNA. Mol Biotechnol 32, 6571.
612 CHEN ET AL.
Stoutjesdijk, P.A., Singh, S.P., Liu, Q., Hurlstone, C.J., Water-
house, P.A., and Green, A.G. (2002). hpRNA-mediated tar-
geting of the arabidopsis FAD2 gene gives highly efcient and
stable silencing. Plant Physiol 129, 17231731.
Szewczyk, E., Nayak, T., Oakley, C.E., Edgerton, H., Xiong, Y.,
Taheri-Talesh, N., Osmani, S.A., and Oakley, B.R. (2007). Fu-
sion PCR and gene targeting in Aspergillus nidulans. Nat Pro-
toc 1, 31113120.
Tang, G., Reinhart, B.J., Bartel, D.P., and Zamore, P.D. (2003). A
biochemical framework for RNA silencing in plants. Gene Dev
17, 4963.
Urban, A., Neukirchen, S., and Jaeger, K.E. (1997). A rapid and
efcient method for site-directed mutagenesis using one-step
overlap extension PCR. Nucleic Acids Res 25, 22272228.
Wesley, S.V., Helliwell, C.A., Smith, N.A., Wang, M.B., Rouse,
D.T., Liu, Q., Gooding, P.S., Singh, S.P., Abbott, D., Stoutjesdijk,
P.A., Robinson, S.P., Gleave, A.P., Green, A.G., and Water-
house, P.M. (2001). Construct design for efcient, effective
and high-throughput gene silencing in plants. Plant J 27, 581
590.
Address correspondence to:
Hua-Fang Wang, Ph.D.
College of Biological Sciences and Biotechnology
Beijing Forestry University
Beijing 100083
P.R. China
E-mail: hfwang@bjfu.edu.cn
Received for publication April 1, 2009; received in revised
form July 2, 2009; accepted July 6, 2009.
RAPID CONSTRUCTION OF PLANT RNAI VECTOR 613
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