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You are here: Home Microbiology Catalase Test -Principle, Procedure and Interpretations
Catalase Test -Principle, Procedure And Interpretations
in Microbiology
It is a basic test to differentiate between Staphylococci and Streptococci. Both are gram positive cocci.
Principle
Catalase is produced by certain bacteria, which
acts as a catalyst in breakdown of hydrogen
peroxide into water and oxygen.
Requirements
Glass rod, test tubes, hydrogen peroxide, test
organisms, control organisms
Procedure
1. Take 2-3 ml of hydrogen peroxide in a test tube
2. Take a colony of test organism with sterile wooden or glass rod and immerse it into hydrogen
peroxide solution.
3. Observe for generation of bubbles. This indicates oxygen production.
Interpretation
1. If bubbles are produced, the organism is catalase positive (Staphylococci)
2. If bubbles are not produced, the organism is catalase negative (Streptococci)
Quality Control
Always have positive and negative control of known organisms, along with the test organisms.
Staphylococcus aureus may be used as positive control and Staphylococcus pneumoniae as negative
control.
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6/11/2014 Catalase Test -Principle, Procedure and Interpretations | howMed
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