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Antibody Expert

Product Analytical
Analysis Services
in Accordance with Regulatory Guidelines

Antibody products should be characterised according to ICH


and FDA guidelines. M-Scan provides a full GLP/cGMP analysis
package for product characterisation and identification of post
translational modifications, which include:

Structural characterisation and confirmation


Amino acid sequence
Amino acid composition
N- and C- terminal sequence
N-terminal sequencing (automated Edman)
MS/MS peptide sequencing
Peptide map
Sulphydryl groups and disulphide bridges
Carbohydrate structure
Monosaccharide composition analysis
Oligosaccharide profiling by HPAEC-PAD and MS
Linkage analysis
Glycosylation site determination
Deamidation
Oxidation

Physicochemical properties
Molecular weight or size
Gel electrophoresis
MALDI-TOF or ES-Q-TOF-MS
Isoform pattern
Extinction coefficient
Electrophoretic pattern
Including CE
Liquid Chromatographic patterns
Reversed Phase
Size Exclusion
Ion-Exchange profiling
Please see the web-site for your local marketing office
Spectroscopic profiles


Circular Dichroism
NMR
www.m-scan.com
Aggregation
Analytical Ultracentrifugation (AUC)
SEC/MALLS
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n

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ES-Q-TOF-MS Analysis of an intact

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m
p
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A
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I
Oligosaccharide population

Please see the web-site for your local marketing office

www.m-scan.com
screening by HPAEC-PAD

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3
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monoclonal antibody.

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9
9
5
6

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Monosaccharide Composition Analysis

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In-depth Analytical Characterisation of

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5

1
A
G
KT
IEL

3870
KK
LK
AA
TP
Q
TV ISV
W PF
SN SP
G ES
KS LQ
SK ST
G G
HV G
FT SA
AP W
C
LV LF
DS
Q NN
YL YF
LT KP

3240
SS ID
VS N

Oligosaccharide population
VT KV
SP KW
TS DI
Linkage Analysis

PW SG
ES T RE
C
TV NQ
G
VN LV
HA SN
NP W
DT
IK
DT Q
FF SD
EP DK
TID TS
EV SY
W SM

2610
G S
FR TLT
SIT TL
DR DK
YE
SN RE
ISQ NH

screening by MS
KL TY
S
PA EC

2246.1
VQ TA
TY AH
PI
PP G
KT
EK IEL
Antibody Products

Q
M RK

2042.4
KA DA
KD V AA
TLS TP
ISV

1980
C
M PF
SID SP

Glycosylation site
ES

1837.3
DK LQ
PD ST
VE G
Q G
SF SA
W W
VF C
DD LF
NN

1395.8 1551.0
determination
EV

1537.5
HV YF
AT KP
Q ID

1426.8
LP N
PA KV
SG KW
DI
AA

1350
SG
TQ RE
SN

100

90

80

70

60

50

40

30

20

10

0
NQ
VM G
LT LV %Intensity
G SN
LC W
KV DT
YG Q
PF SD
PE DK
TV TS
TV SY
W M
SN SS
G LT
LS IEC
SS IP
G KV
HV FS
FT
AP RN
LV EG
Q C
DS
YL
LT
SS
VS
VT
SP
TS
PW
ES T
TV
NC
AV
PH
SA
TS
VK
KD
IK
QVTWNSGSKKSGVHTFPAVLQSDLYTLSSSVTVPSSTWPSETVTCNVAHPNKITDFFPEDITVEWGRFTISRDNSQSILKAPQVYTIPPPKEQMAKDKVSLTCMDISKDDPEVQFSWFVDDVEVHTAQPLAPGSAAQTNSMVTLGCLVKGYFPEPVTVTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVPSSTWPSETVTCNVAHPASSTKVDKKIVPRDCGCKPCICTVPEVSSVFIFPPKPKDVLTITLTPKVT CVVVDISKDDPEVQFSWFVDDVEVHTAQTQPREEQFNSTFRSVSELPIMHQDWLNGKEFKCRVNSAAFPAPIEKTISKTKGRPKAPQVYTIPPPKEQMAKDKVSLTCMITDFFPEDITVEWQWNGQPAENYKNTQPIMDTDGSYFVYSKLNVQKSNWEAGNTFTCSVLHEGLHNHHTEKSLSHSPG
QVTWNSGSKKSGVHTFPAVLQSDLYTLSSSVTVPSSTWPSETVTCNVAHPNKITDFFPEDITVEWGRFTISRDNSQSILKAPQVYTIPPPKEQMAKDKVSLTCMDISKDDPEVQFSWFVDDVEVHTAQPLAPGSAAQTNSMVTLGCLVKGYFPEPVTVTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVPSSTWPSETVTCNVAHPASSTKVDKKIVPRDCGCKPCICTVPEVSSVFIFPPKPKDVLTITLTPKVT CVVVDISKDDPEVQFSWFVDDVEVHTAQTQPREEQFNSTFRSVSELPIMHQDWLNGKEFKCRVNSAAFPAPIEKTISKTKGRPKAPQVYTIPPPKEQMAKDKVSLTCMITDFFPEDITVEWQWNGQPAENYKNTQPIMDTDGSYFVYSKLNVQKSNWEAGNTFTCSVLHEGLHNHHTEKSLSHSPGK
K
VD
S STK
PA
AH
NV
TC
ETV
PS
STW
PS
TV
SV
SS
TL
LC
SD C
VL
Q NE
NR

Microheterogeneity
PA
TF SF
VH KTK
G TH
SS EA
SL
PG-COOH
G TC
NS SM
TW STC
TV
PV KD
PE DS
YF TD
Q
KG

at C-terminus
W
CLV NS
G VL
VTL NG
SM RQ
SE

Disulphide Bridge
TN
AQ G
SA KID
ETVTCNVA
G W
AP VK
PL DIN
Q PK
TA FY
VH
VC NN
PGK-COOH
FL

-S-S-
DD C
FV AS
W
W
FS G
G
Q
EV LTS
DP SE
Q
KD PS
DIS IFP
M VS
TC C
VSL AP

Analysis
DK DA
RA
RNEC
AK IK
M LE
EQ TK
PK AG
PP TH
YTI EA
C
PQ YTC
KA NS
SIL RH
Q YE
NS DE
RD TK
TIS

min
RF TLTL
G SS
M
EW YS
ITV SC
ED KD
FP DS
DF Q
KIT W
TD
PN

22.5
AH NS
VL
NV NG Lysine
TC Q
ETV SE
C
PS G
Reagent
STW KID
PS W
TV VK
SV
Phenylalanine
SC DIN
PK

20
TL
LY FY
SD NN
Norleucine
Q FL
VL C
PA
Leucine
W
TF AS
Isoleucine
VH G
G
G
KS LTS
Cystine
SK C
Q

17.5
G SS
NS PP

Ion-Exchange chromatographic
VTW SIF
Q TV Methionine
TA
LTV
SS
SP Valine
TQ

MS/MS Peptide Sequencing


M
DIV

15
Tyrosine

Amino Acid Composition

12.5
MS Peptide MAPPING
Gas Phase N-terminal
Proline
Alanine
Threonine
Arginine

10
Histidine
Glycine
Serine

7.5
H2N-DIVMTQ

Sequencing
Glutamic Acid
Aspartic Acid

profiling

5
2.5
600

500

400

300

200

100
mAU

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