Documente Academic
Documente Profesional
Documente Cultură
The new Melon™ Gel Monoclonal IgG Purification Kit from Pierce offers a fast and simple
protocol for purifying IgG from cell culture supernatants and ascites that renders Protein A
and Protein G methods obsolete
How does Melon™ Gel work? Highlights:
Melon™ Gel is composed of a proprietary combination of ligands that • Fast – purify antibodies in half the time of Protein A or Protein G
retains the component proteins typically found in serum and allows • Efficient – recovery and purity is often better than Protein A or
IgG to pass through. Protein G purification
• Robust – effective for antibodies known not to bind to Protein A or
The Melon™ Gel Monoclonal IgG Purification Kit: Protein G
• Replaces tedious bind-and-release antibody-isolation methods, • Gentle – antibody retains activity because it is not exposed to
resulting in a fast, easy and gentle approach to antibody recovery denaturing conditions
and purification • Regenerable – support can be used for up to six purifications
• Delivers monoclonal antibodies (MAbs) with quality and purity • Compatible – buffer is amine-free and does not require removal
equal to – or better than – that obtained using classical Protein A or neutralization
or Protein G purification methods • Flexible – accommodates a broad range of sample volumes
• Purifies monoclonal IgG from up to 1 L of cell culture supernatant Cell culture: Spin columns can process supernatant samples that
(serum-containing or serum-free) and up to 200 ml of ascites fluid are < 30 ml. Filter flasks allow processing of samples up to 1 L.
Ascites: Spin columns process samples up to 6 ml.
Figure 1. Melon™ Gel offers better recovery and purity from cell culture super- Figure 2. Melon™ Gel offers better recovery and purity from ascites fluid than
natant than Protein G. IgG was purified from cell culture supernatant containing Protein G. IgG1 was purified from ascites fluid, resolved by SDS-PAGE and detect-
10% fetal bovine serum (FBS), resolved by SDS-PAGE and detected with Imperial™ ed with GelCode™ Blue Stain Reagent (Product # 24590). Lane 1: Original ascites
Protein Stain† (Product # 24615). Lane 1: Original cell culture supernatant, Lane 2: fluid, Lane 2: treated with Ascites Conditioning Reagent (Product # 45219) and
IgG after precipitation with Saturated Ammonium Sulfate (Product # 45216), Lane purified with Melon™ Gel, Lane 3: Protein G column flow-through, Lanes 4-5:
3: Melon™ Gel-purified IgG and Lanes 4-6: Protein G-purified IgG. Protein G column washes and Lanes 6-8: Protein G column elutions. NOTE: The
Protein G-purified sample is contaminated with transferrin (Lanes 6-8). The
Melon™ Gel-purified sample is not (Lane 2).
www.piercenet.com/melg92h
Cell Culture Supernatant Purification Ascites Purification
Slide-A-Lyzer® 2
1. Combine the Ascites 2. Centrifuge sample at
1
Cassette
U.S. Pat.
U.S. # 5,503,741
Pat. # 5,503,741
1
Cassette
3
U.S. Pat.
U.S. # 5,503,741
Pat. # 5,503,741
Tel: 815-968-0747 or 800-874-3723 • Fax: 815-968-7316 • Customer Assistance E-mail: CS@piercenet.com • www.piercenet.com # 1601296 09/05
© Pierce Biotechnology, Inc., 2005. Pierce products are supplied for research or manufacturing applications only.