Genetics science concerned with heredity & variation
Bacteria are haploid Reproduction Asexual mode most common & important in bacterial growth cycle (e.g. fission) no genetic recombination genetic recombination exchange of genes between !"A molecules to form combination of genes in a chromosome changes arise through mutations mutation change in the base se#uence of a cell$organism w$c may occur naturally or through induction by agents %&exual' mode bacteria are not differentiated to somatic or gametic cells functional$mating types( a. donor ()male*) b. recipient (female*) involves a unidirectional transfer of a portion of !"A from donor to recipient gene transfer + genetic recombination genetic recombination + changes changes + variations "atural methods of genetic transfer A. ,on-ugation transfer of genetic information via cell.to.cell contact plasmids . small/ circular !"A that can exist independently of host chromosome0 found in bacteria0 also in yeast episome plasmid that can exist with or w$o being integrated into host's chromosome con-ugative plasmids have genes for 1 factor 1 factor )fertility* factor . genes for cell attachment/ sex pili/ transfer 1 2 x 1 . mating 1 2 + contains extrachromosomal 1 factor (donor) 1 . + no 1 factor (recipient) 3fr x 1 . mating 3fr strain + high fre#uency of recombination + 1 factor integrated into bacterial chromosome + donor MCB 1 - wtcruz 10 Bacterial Genetics 2 4. 5ransduction transfer of bacterial genes by viruses . most common mechanism 6inds of transduction 1. Generali7ed . occurs during the lytic cycle of the virulent & temperate viruses . host !"A derived from any portion of the host genome becomes a part of the !"A of the virion in place of the virus' genome . &peciali7ed or restricted . occurs only in some temperate viruses . involves the transfer of specific region of the host chromosome into the virus replacing some of the viral genes ,. 5ransformation . the upta8e by a cell of a na8ed DNA molecule (cell.free) or fragment from the medium and the incorporation of this molecule into the recipient chromosome . contact with competent cell (one that is able to ta8e up !"A) 9rotoplast 1usion use in microorganisms with ": 8nown con-ugation system en7ymes are used to digest cell wall to produce protoplasts MCB 1 - wtcruz