Biology Lab Report

Test Nutrient in Food

Date of Pactical:
January 21
st
2014














Collection Time Teachers Sign Final Score





Contents

Cover.......................................................................................................... i

Contents..................................................................................................... ii

Part I INTRODUCTION
1.1 Purpose.......................................................................................... 1
1.2 Theoretical Basis............................................................................ 1

Part II MATERIALS and METHODS
2.1 Materials........................................................................................ 1
2.2 Methods......................................................................................... 1

Part III RESULT and DISCUSSION
3.1 Observation................................................................................... 1
3.2 Discussion...................................................................................... 1

Part IV CONCLUSION
4.1 Conclusion..................................................................................... 1

BIBLIOGRAPHY...........................................................................................

Attachment of the answer........................................................................











Part 1 INTRODUCTION

1.1 Purpose
o Complete the task of Biology
o Observed the content of nutrients (carbohydrates, protein, fats) in various
foods

1.2 Theoretical Basis
Carbohydrate is the main energy for living things especially for humans, it can
be rice, maize, cassava, potatoes and wheat. If you want to prove the existence
of a substance starch on foods then you can used Lugol or Iodine (both are
liquid). Lugol/Iodine and extract food which containing carbohydrates are mixed,
the liquid will turh in a blue color.
Protein, it can be eggs, milk, fish, meat. If you to prove the existence of a
substance starch on foods, then used Biurete, when biurete and foods that
contain protein extract are mixed the color of that biurete will turn into purple.
Fat, a source of fat fo example: cooking oil, meat, cheese, peanuts and butter.
If you want to prove the fat content in foods, you can shed a little extract of the
food to the paper, if that foods contain fat there will be a transparent stain.
Source: www.triansafira.blogspot.com/2012/02/laporan-biologi.html



















Part 2 MATERIALS and METHODS

2.1 Materials




Water newspaper/oil paper foods Pipette




Drop plate Mortar and Grinder Spatula




Biuret Iodine Reaction tube and shelf










2.2 Methods
Preparation/ before test nutrient :
1. Grind food until its fine using mortar and grinder.
2. Put each food to drp plate (2) and tube (1)
Test Nutrient :
1. Amylum test : Drop food which already fine in drop plate using spatula, put a little
Iodine into it and see the changing color.





Iodine
2. Protein test : Drop food (in tube) with biuret. Observe!





Empty tube Biure tube already filled
with little biuret
3. Lipid test : Spread food to newspaper/oil paper. Observe









Part III RESULT and DISCUSSION

3.1 Result
Food Iodine Biuret Newspaper
Rice + - -
Fried tempe - + +
Egg - + -
Bread + + -
Margarine - - +
Oil - - +
Tahu - + -

Note :
If the solution of food given iodines solution changes to dark blue and then
the solution containing starch
If the solution were given food and biuret solution turned purple then the
solution containing protein
If the paper were given a food and the paper proved to be transparent then
the solution containing lipid

3.2 Discussion
In the lab activities we use reagents that are used to determine the content of
foods, among others :
Iodine used to test whether a food containing carbohydrate (strach) or not. When
the food we used as drops of iodine blackened, then the food contains
carbohydrates. If the color are darker means the food contains a lot of
carbohydrates.
Biuret reagent is used to test the protein content. When foods contain proteins
that react with the biuret it will produce the purple color/ violet color.
Scrap paper is the basis for examining the fat content. Because newsprint easily
absorb water/oil so its suitable for this test. In testing these fats in food that has
been mashed in the rub on scarp paper after it is heated above the burner so that
the water content is easy spirtus mongering, if there is a transparent stain that these
foods contain fat.
o Carbohydrate test
From the observation by conducting research can be seen that
the substance contains starch are rice and bread
o Protein test
Raw foods containing are protein are tempe, egg,bread, tahu
Lipid test
Once it is tested with opaque paper to see its fat content result. Raww
foods containing fat are fried tempe, margarine, oil

Part IV CONCLUSION
4.1 Conclusion
From these experiments we know that in order to determine wether there is any
substance content carbohydrate, protein and fat in the foods we know to test food
with lugol test, biuret test and paper test with opaque fat. We know that each food
contain different nutrient and different function fo humans body.


























BIBLIOGRAPHY
www.triansafira.blogspot.com/2012/02/laporan-biologi.html
http://cuk-ing.blogspot.com/2013/05/bab-i.html