ISOLASI SENYAWA BIOAKTIF FLAVONOID BERBASIS

METODE SPEKTROFOTOMETRI DAN GC-MS PADA

PROPOLIS
Rido Maulana
1)
, Riyan Sopiyan
1)
,Yuli Triretno
1)
, Khonsa Hartsu Syuhada
1)
1
Mahasiswa Fakultas Kedokteran dan Kesehatan Universitas Muhammadiyah
Jakarta

ABSTRAK
Objective :
Flavonoid merupakan salah satu golongan senyawa bioaktif utama yang terdapat
dalam Propolis dan mempunyai potensi sebagai antiinfalamasi, antioksidan, dan
antimikroba. Untuk memaksimalkan potensi Propolis, maka diperlukan penentuan
kadar flavonoid total sebagai penanda senyawa bioaktif utama yang terdapat
dalam Propolis.
Material & Method :
Penelitian ini menggunakan dua metode analisis flavonoid, yaitu dengan metode
spektrofotometri dan GC-MS. Penggunaan metode GC-MS adalah untuk
menganalisis jumlah senyawa secara kuantitatif dan spektrometri massa dan
menganalisis struktur molekul senyawa analitif.
Result
GC-MS analisis mengungkapkan bahwa propolis mengandung 23 senyawa aktif.
Kadar senyawa tertinggi adalah galangin flavone (R
t
-13.78) yang mana nama
lainnya adalah flavonoid.
Conclusion


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