WATTAgNet.

com NOVEMBER/DECEMBER 2012

Digital edition: www.fi-digital.com

Feed companies find success

in difficult times

Six points for

optimal use of
soybeans in piglet feed
Advances in functional petfood ingredients
Using enzymes in poultry feed to increase profits
20
YEARS
 WATTAgNet.com NOVEMBER/DECEMBER 2012 |1
Volume 33 Number 7

Features
6 Feed companies find success
in difficult times
Although volatile commodity prices continue to
vex the global feed market, some companies are
nonetheless managing to push ahead.

10 Six points for optimal use

of soybeans in piglet feed
Soybeans are an obvious feed choice for pig diets.
However, formulating for piglets can be tricky.

12 Advances in functional
petfood ingredients
Prebiotics, probiotics and their combinations –
synbiotics – promise health benefits for pets.

16 Using fiber in veal feed to improve growth

Supplementing veal
calves’ diets with
short-chain fructo-
oligosaccharides can
potentially offer benefits
to both feed producers and
Cover picture: Lincoln Rogers/Fotolia.com breeders.

Departments 20 Betaine Hydrochloride: functional

nutrient in the nutrition of broilers

4 Feed world news Historically, betaine has been used in poultry feed in
its anhydrous form, extracted from beets. However,
there may be a smarter and less-expensive choice.

28 Products
24
40 Marketplace
Using enzymes in poultry feed
to increase profits
While alternative ingredients are an economical option

40 Advertisers’ Index
for feed producers, nutritive value can suffer in the
process. Careful use of enzymes may offer a solution.

November/December 2012 | WATTAgNet.com

2|
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4|
Feed world news
Stay on top of what’s happening!
For the latest news, go to: www.WattAgNet.com/LatestNews.aspx
▶ Norel partnered
with Latvian wholesale ani-
mal feed additive supplier
Proventus to present “The
use of additives in poultry,”
organized for an audience of
poultry producers in Belarus.
▶ The National Grain and
Feed Association announced
that Shannon Gombos
joined its staff as manager
of arbitration and mediation
services.
▶ The Institute of Feed
Education and Research has
named Jim Sullivan Ph.D.,
as the fi rst executive director
Market Data expanded to of the charitable foundation,
which seeks to sustain the
include more markets and data sources future of food and feed pro-
The Market Data section of WATTAgNet has been expanded to duction through education
include a much broader range of key indicators for the worldwide and research.
poultry, feed and pig markets. Market Data was launched in 2011 to
give WATTAgNet.com users quick access to statistics impacting the ▶ The National Grain and
Feed Association appointed
U.S. poultry market. It has been popular with users of the site, and Heather McElrath as its di-
additional U.S. and international statistics will broaden its appeal. rector of communications.
It has been expanded to include:
▶ Listings of leading poultry, pig and feed companies, ▶ Dr. William Kunkle,
both U.S. and internationally. Ph.D., has been posthu-
▶ Expanded U.S. poultry statistics, including mously inducted into the
American Feed Industry
turkey production numbers. Association’s Liquid Feed
▶ International poultry statistics. Hall of Fame, the highest
▶ U.S. and international pig production numbers. award offered by the AFIA
▶ In depth worldwide poultry data from the annual Liquid Feed Committee and
WATT Executive Guide to World Poultry Trends. the liquid feed industry.
▶ More statistics to come.
Access to the Market Data section of WATTAgNet.com requires
▶ Dong Bui Van has been
appointed the new country
registration. Registration is FREE and can be completed in less than sales manager at Nutriad
a minute. Comments on these expanded features are welcome, and Vietnam.
can be directed to bplantz@wattnet.net.
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WATTAgNet.com | November/December 2012
▶ MSD Animal Health
(known as Merck Animal
Health in the U.S. and
Canada) received registra-
tion for zilpaterol hydro-
chloride, the active ingre-
dient of Zilmax in two key
South America beef pro-
ducing countries — Brazil
and Peru.
▶ Delacon appoint-
ed Peter Elek as managing
director of Delacon Hungary.
▶ Excentials published
its R&D program’s insights
on the differentiation in be-
taine and organic selenium
sources at the 24th World’s
Poultry Congress.
▶ Novus International
Inc. has unveiled a new de-
sign in its product packaging
that includes enhanced han-
dling features.
▶ The International
Fishmeal and Fish Oil
Organisation gained the
100th factory to be certi-
fied to its global standard
for responsible supply at
the Corral factory, south of
Chile.
CALENDAR
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industry events:
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6|
Markets by Ken Jennison
Feed companies find
success in difficult times
Although volatile
commodity prices
continue to vex the
global feed market,
some companies are
nonetheless managing
to push ahead.
Coping with commodity prices is by far the
largest hurdle that feed producers are fac-
ing at the close of 2012, no matter where
they are located. However, throughout the
world a number of feed companies are
choosing to look beyond current drought
conditions and are using the time to ex-
pand operations through mergers, capital
expenditures and other initiatives.
Efficiency in Italy  to continue distributing its feed on the
The Veronesi Group
is the largest animal feed
producer in Italy. The first animal
feed production plant belonging to the
Group was built in 1958 by the family pa-
triarch Apollinare Veronesi. An additional
six feed-producing factories flank the one
originally set up more than 50 years ago in
Quinto di Valpatena.
Despite tough market conditions, in the
past year Veronesi Group has succeeded
in increasing its compound feed production
by 2 percent to almost 3 million metric tons.
The group notes that in the last five years, it
has increased its production from 2.4 million
metric tons to 2.965 million metric tons us-
ing the same seven plants – a feat accom-
plished entirely through efficiency initiatives.
Acquisition in Belgium 
In 2011, the Belgian
feed market grew by ap-
proximately 4.08 percent
(over 2010) to 6,709,925 metric tons. Of
that, the Aveve Group captured a market
share of 21.29 percent, having increased
its volume by 4.39 percent to 1,428,481
metric tons during that same period. The
Aveve Group’s increase was fueled in part
by 71,200 metric tons from its French sub-
sidiary Sabe, and also by an acquisition in
Germany – Mischfutterwerke Mannheim.
In August of 2011, the Aveve Group
acquired the former production plant of
Muskator GmbH in Mannheim. The plant
has an annual production capacity of
120,000 metric tons – 50 percent cat-
tle feed, 20 percent pig feed, 20 percent
poultry feed and 10 percent pet food
(Ovator). In 2006, Muskator produced
about 500,000 metric tons of feed in five
plants and ranked 14th among the major
German feed manufacturers. As part of the
acquisition, the Aveve Group was allowed
southwestern German market under the
Muskator/Ovator trademarks.
Investment, strategic
agreement in Russia 
Miratorg Agribusiness
Holding is the largest pork
producer in Russia, with its pig operations
requiring approximately 720,000 metric
tons of feed in 2011. Miratorg has already
invested to increase its annual feed pro-
Photo by Lincoln Rogers/Fotolia.com
duction capacity from 270,000 metric
tons per year to 630,000 metric tons and
is slated to open another feed mill, its third,
before the end of 2012.
When the new plant is completed,
Miratorg says it will be able to produce
about 1.1 million metric tons of feed per
WATTAgNet.com | November/December 2012

year, which will make it the largest feed
manufacturer in Russia. The company’s
current feed mills are in Ivnyanskom and
Prokhorovka, which are located in the
Belgorod region. The new mill will also be
in Prokhorovka.
In Luxembourg, Sodrugestvo Group
S.A. also made news this past year when it
signed an agreement with Japanese trad-
ing company Mitsui & Co. Ltd. to become
strategic partners in some of its business
activities, with Mitsui becoming a 10-per-
cent shareholder of Sodrugestvo. Although
the cost of the shares has not been dis-
closed, it has been noted that the transac-
tion was based on an enterprise value of
$2.2 billion for Sodrugestvo.
According to information released
by Sodrugestvo, the primary points of
the partnership include an alliance in the
origination, export and marketing of grain
from Russia and other former Soviet Union
countries; support for Mitsui’s participa-
tion in the feed and livestock business in
Russia; and a collaboration in the soybean
origination, processing and distribution
business in Brazil between Sodrugestvo’s
Alianca joint venture and Mitsui’s Multigrain
grain farming and logistics subsidiary.
Aqua expansion in Egypt
The Danish fish feed
company Aller Aqua Group
expanded its production
outside of Europe for the first time this
year by taking a 60 percent shareholding
in Zoocontrol in Egypt. The re-named
Aller Aqua Egypt intends to expand by
building a second feed plant next to the
existing Zoocontrol mill. Aller Aqua is a
leader in feeds for freshwater fish in Europe
with mills in Germany, Poland and
Denmark. Until this year, the com-
Coping with commodity pany exported products to other
markets in the Middle East, Asia and
prices is the largest hurdle North Africa.
Other recent moves by Aller
that feed producers Aqua include establishing a sales
office in China and developing
are facing at the close a presence in Norway, Europe’s
largest single aquaculture market.
of 2012, no matter where Zoocontrol remains owned by its
founders, the Mansour family.
they are located.
November/December 2012 | WATTAgNet.com
|7
Building in the
Philippines
In March 2012, San
Miguel Foods began con-
struction of a new feed mill in Villanueva,
Mindanao. San Miguel Foods is the larg-
est Filipino-owned feed company in the
Philippines and currently operates 41
feed mills in the country.
The new mill is set to be operational by
2013 and will have a capacity of 60 metric
tons per hour, which will make it the com-
pany’s largest mill. According to Norman A.
Ramos, vice president and general manager
for San Miguel Foods, the new plant will give
opportunities to local farmers who would
be supplying 11,000 metric tons of corn and
5,000 metric tons of cassava each month.
Cargill expansion in
Vietnam
Also in March 2012,
Cargill Vietnam, a wholly
owned subsidiary of Cargill Inc. USA,
began construction of its ninth feed mill in
Vietnam. The mill will have a production
capacity of 240,000 metric tons per year
and is slated to be operational in the latter
half of 2013. The facility will bring the com-
pany’s milling capacity to 1 million metric
tons per year.
Located in Dong Van II Industrial Zone
in Ha Nam province, the company has
invested $18 million to create the facility.
Once operational, it is expected that its
output will help the company increase its
market share of animal feed in Vietnam to
10 percent.
Investment in Thailand
Thai agribusiness in-
tegrator Betagro Group
announced earlier this year
that it will be investing in feed produc-
tion in neighboring countries with an eye
to market growth expected from the
establishment of the Asean Economic
Community trading pact, set to launch in
2015. Betagro plans on investing EUR7.33
million in feed, food and livestock busi-
nesses over the next five years.
One project Betagro has planned is a
feed mill in Cambodia, which will be com-
8|
Success
missioned in early 2013 at an investment
of EUR12.2 million. The mill will operate
with a capacity of 12,000 metric tons per
year, though Betagro has plans to increase
its production to 24,000 metric tons as its
Cambodian operations expand.
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Expansion and
investments from China 
Activity is nearly constant
in China, as a number of
companies look to increase market share
there. Da Bei Nong Group launched an
ambitious investment plan at the beginning
of 2012, which included RMB318 million for
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www.WATTAgNet.com/153436.html 
the acquisition, construction or expansion
of production lines in five different cities –
Shenyang, Kiamusze, Chongqing, Yichun
of Jiangxi province and Chifeng of Inner
Mongolia. According to the plan, a subsidi-
ary of Da Bei Nong Group will purchase
Shengyang Yingda Technology Co. Ltd. for
RMB38 million. An additional RMB93.78
million will then be invested to bring the
production capacity of the facility up from
60,000 metric tons annually to 240,000
metric tons of compound feed a year.
Other investments by the Group include
RMB53.93 to establish a pig feed production
line with a capacity of 12,000 metric tons per
year in Kiamusze, RMB65.66 million for an
18,000-metric-ton pig feed production line in
Chongqing city, and a 12,000-metric-ton pro-
duction line in Yichun city and in Chifeng city.
Another company, the New Hope Group,
China’s largest feed producer, entered into
an agreement earlier in 2012 with Japanese
grain trader Marubeni Corp. to expand in
Africa, the Middle East, Eastern Europe and
South America. According to the company’s
website, New Hope Group has been expand-
ing its overseas business in the face of fierce
competition in the animal feed business in
China. New Hope Group currently has 16
feed factories overseas and plans to add four
additional plants annually, according to Tang
Yong, a company director. Each overseas fac-
tory will have a production capacity between
100,000 and 200,000 metric tons.
The company has completed the con-
struction of an animal feed factory in Egypt
that will soon become operational, accord-
ing to Tang. It will also begin work on a
plant in Johannesburg, South Africa.
The profitability of New Hope’s over-
seas business is strong, according to Jiang
Xiaodong, an analyst with Huatai United
Securities Co. Ltd. “Demand in Southeast
Asian countries is increasing rapidly, but
their animal feed businesses lag behind,”
Jiang Xiaodong said in a research note.
”The company will quicken its expansion in
overseas markets in the future.”
WATTAgNet.com | November/December 2012
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10 |
Ingredients by Ioannis Mavromichalis, Ph.D.
Six points for optimal use
of soybeans in piglet feed
Soybeans are an
obvious feed choice
for pig diets.
However,
formulating
for piglets
can be tricky.
No matter how expensive feed ingredients
become, soybeans remain one of the most
economical sources of protein in pig diets.
Soybeans are cultivated for their high oil
content, because their protein is of good
quality, and because they complement the
protein of cereals so well that this blend
has become the ideal feed for most pig
diets worldwide.
However, like most other feedstuffs,
soybeans are not without their problems.
The major issue with soybeans is the pres-
ence of a great number of anti-nutritional
factors that reduce digestibility and, even-
tually, growth, productivity and profit-
ability, even if fed to mature pigs. Unless
soybeans are thermally processed very
well, these anti-nutritional factors are not
destroyed easily. Unfortunately, with over-
cooking also comes denaturation of the
useful proteins, something that defeats the
purpose of cooking in the first place. Thus,
optimal processing conditions are required
to obtain a balance between protein quality
and reduction of anti-nutritional factors.
These anti-nutritional factors have the
most impact in young pigs, especially those
around the time of weaning. In this case,
things become even more complicated. As
Like most feedstuffs, soybeans are not without their problems.
it happens, the otherwise useful soybean
proteins cause an allergic reaction that trig-
gers the gut immune system as if it were in-
vaded by pathogenic microorganisms. This
negative reaction leads to inflammation and
diarrhea. Quite often the inflammation dam-
ages the lining of the intestines, thus greatly
facilitating the attachment of pathogens and
the absorption of their exotoxins. Although
the whole effect lasts no more than a week,
until the immune system develops “immu-
nity,” the damage is long-term and multi-
faceted when complicated with pathogenic
bacterial infection, as is the case more often
than not.
Two schools of thought  Gradual adaptation
To combat this problem, two schools of
thought have evolved. One calls for the in-
troduction of soybean protein immediately
after weaning (assuming piglets had no or
minimal exposure to pre-weaning creep
feed). Although the introduction of soybean
protein post-weaning is recommended to
be a gradual process, this method requires
the presence of about 10 percent soybean
meal in the first diet post-weaning, increas-
ing rapidly to 15 percent in the second
diet and so on. This approach requires a
high level of feed intake to ensure minimal
impact as the negative effects of soybean
protein tend to be more severe when feed
intake is below maintenance requirements.
The benefits of this program are 1)
low-cost formulas, 2) rapid adaptation to
soybean protein, and 3) minimal number
of diet changes. In contrast, the negative
aspects include higher chances of diarrhea
outbreaks, especially in farms with below
ideal health status – which is the case for
many places worldwide.
 
A more gentle approach to the same
problem calls for zero levels of any kind
of soybean products in the first diet post-
weaning, followed by a second diet with
low levels of soybean protein in the form of
refined products (such as soy protein iso-
late that has virtually no anti-nutritional fac-
tors). Here too, the key phrase is gradual
adaptation, but the main difference is the
WATTAgNet.com | November/December 2012

disassociation of the weaning stress from
the soybean protein issue. Quite often,
these two problems are treated as one and
the same, but obviously they are not, no
matter how closely related. This second
approach is suitable for farms with health
issues, but resulting diets are always more
expensive than can be afforded, and thus
Photo by Vasilius/Fotolia.com
less desirable from a market point of view.
Of course, between these two extreme
approaches there are many variations that
have been developed and applied with var-
ying degrees of success. In my opinion, and
what I use in my own piglet products and in
my consulting service, the middle road has
many merits and appears most suit-
able for products that will be used in
farms with an undetermined
health status. In summary,
the following six points
have shown the best
results in my prac-
tice.
1. Some form
of soybean
protein should be present in the first diet
post-weaning (and also in the creep feed if
any is used). The optimal form is that of full-fat
extruded soybeans, but it is rather difficult
to find extruded soybeans that have been
processed correctly. Thus, if the total level
of trypsin inhibitor activity, the best index for
soybeans to be used in piglet diets (see Figure
1) is below 10 mg/gram, then up to 10 percent
of soybeans can be used without problems.
In the exceptional case where trypsin inhibitor
activity levels are found to be below 5 mg/
gram without “burning” the protein, then up
to 20 percent can be used. I have formulated
diets with 30 percent extruded soybeans,
but such levels are not required unless the
diet cannot contain fish meal or other such
sources of high-quality protein.
2. When extruded soybeans are not avail-
able or do not have low enough trypsin
inhibitor activity levels, the next most suitable
and economical soybean protein source is
that of soy protein concentrate (or isolate,
depending on which is less expensive per
unit of protein). This ingredient is usually very
refined and can be used up to 20 percent
in most piglet diets without problems, apart
from that of increasing cost.
November/December 2012 | WATTAgNet.com
| 11
FIGUREFigure
1: Effect of soybean
1: Effect Trypsin
of soybean Inhibitor
Trypsin Activity
Inhibitor Activity
intake on piglet growthintake on piglet growth*
700
630
Growth (g/day)
560
490
420
350
0.5 1 2 3 4 5 6
TIA intake (g/day)
If the total level of trypsin inhibitor activity is below 10 mg/gram, then up to 10 percent of
soybeans can be used without problems.
RELATED STORIES:
3. In the first diet post-weaning, the
combined maximum level of crude
protein coming from extruded soybeans or
Identifying low-cost
high-quality piglet feed,
soy protein isolate should not exceed 10 www.WATTAgNet.com/153438.html 
percent (a rule of thumb I use quite often).
This ensures the immune system is trig-
gered sufficiently but not overloaded with
soybean protein antigens. system matures, while the immune sys-
tem is allowed enough time to cope with
4. To minimize this allergenic reaction,
diets should be made as palatable
the allergenic reaction.
and digestible as possible. To this end,
immunoglobulins (from plasma or eggs),
fishmeal and cooked cereals also help in
6. In the third diet, and any such formula
that may follow it, only soybean meal
is used as the main source of protein to
promoting early feed intake. In addition, keep costs down.
the diets should always contain suitable If the above guidelines are followed,
anti-microbial agents (not necessarily the and feed intake is sufficiently adequate,
so-called antibiotics now banned in many the allergenic reaction will pass virtually
countries). unnoticed and the pigs will not suffer
much from the presence of anti-nutritional
5. The second diet post-weaning
should contain about half the
factors during one of the most sensitive
periods in their lives. Although there are
amount of the ingredients used in the
first diet. The difference should be made
by using soybean meal (the 48 percent
other sources of soybean protein, in my
experience, the above simple ingredients
offer the required balance among cost,
protein soybean meal works best here as quality and performance in today’s chal-
it contains less fiber than the 44 percent lenging marketplace.
type). Roughly, 10 percent soybean meal
will provide the required balance in soy
protein. In this way, the level of anti-nutri- Ioannis Mavromichalis is the nutrition
tional factors continues to gradually rise editor for WATT. He can be reached at
in the post-weaning diets as the digestive nutrition@wattnet.net.
12 |

Petfood by Debbie Phillips-Donaldson

Advances in functional
petfood ingredients
e e t s bananas
Prebiotics, probiotics and their S u ga r b
combinations—synbiotics—
promise health benefits for pets FIGURE 1: Digestibility of nutrients in study
of prebiotics, probiotics and synbiotics
Dietary groups P
Since the term “functional food” was first used in Japan
Attribute Control Prebiotic Probiotic Synbiotic SEM value
in the 1980s, food science and nutrition knowledge have
progressed from identifying and correcting nutritional de- Dry matter 79.16ab 78.13a 80.45b 80.08b 0.523 *
ficiencies—improving life expectancy—to designing foods Protein 77.31a 76.08a 77.65a 80.44b 0.538 **
that promote optimal health and reduce risk of disease— Fat 87.85a 89.72a 92.67b 92.97b 0.792 **
improving wellness and the quality of life, said K.B. Kore, Fiber 28.80a 32.62ab 35.60b 37.37b 1.529 *
PhD, assistant professor with the College of Veterinary
CHO (NFE) 85.24 84.13 86.39 84.57 0.888 NS
Science and Animal Husbandry at Navsari Agricultural
University in India. Calcium 48.10a 54.31b 52.55b 60.18c 1.223 ***
This progression began in human food, but it soon Phosphorus 52.23 52.80 53.45 54.96 1.378 NS
made its way into petfood, prodded by consumers’ de- abc = Differences were significant, *p<0.05, **P<0.01, ***P>0.001
mands for products for their furry family members with the Source: Dr. K.B. Kore

same types of ingredients that foods for humans have. In
many cases, this means prebiotics and probiotics.
In a Petfood Forum Asia 2012 presentation, “Prebiotics,
Probiotics and Synbiotics as Functional Foods for Dogs,” Dr. Kore
shared the potential health benefits of these functional ingredients:
▶ Modulation of hindgut health (mainly colon), including maintain-
ing intestinal microbial balance and lowering pH, production of
organic acids and ammonia;
▶ Resistance to intestinal infection;
▶ Improvement in bioavailability and absorption of minerals;
▶ Hypoglycemic and hypolipidemic actions;
▶ Management of infectious diarrhea;
▶ Reduction of risk of obesity; and
▶ Reduction of risk and severity of osteoporosis, colon cancer
and inflammatory bowel disease.
An evolving concept
Dr. Kore offered an evolving concept of a prebiotic: a selec-
tively fermented ingredient that allows specific changes, in both
the composition and the activity of gastrointestinal (GI) microflora,
that confers benefits on host health and well-being (Gibson et al.,
2004). This builds on a previously accepted definition of prebiotics
as non-digestible ingredients that benefit the host by selectively
stimulating the growth or activity of one or a limited number of
bacteria (commonly considered probiotics) in the colon.
Results of a study on prebiotics, probiotics and synbiotics in
dogs, conducted by K.B. Kore, PhD, and colleagues at the Cen-
tre of Advanced Faculty Training in Animal Nutrition at the Indian
Veterinary Research Institute, showed digestibility differed based
on the functional ingredients fed.
Dr. Kore added that an ideal prebiotic beneficially alters luminal
or systemic aspects of the host’s defense system. Prebiotics that
have been researched and used in both human foods and pet-
foods include inulin, fructo-oligosaccharide (FOS), oligofructose
(short chain, or scFOS), galacto-oligosaccharide (GOS) and lactu-
lose from sources such as garlic, onions, Jerusalem artichokes,
chicory roots, sugar beets, milk (lactose) and bananas. Dr. Kore
also shared potential new prebiotics available on the horizon for
petfoods:
▶ Xylo-oligosachharide, an oligosaccharide made of xylan through
enzymatic hydrolysis (commercially produced with corn cobs);
▶ Isomalto-oligosaccharide, a mixture of short-chain carbohy-
drates with digestion-resistant properties found naturally in
some foods and also manufactured from starch; and
▶ Soybean oligosaccharide,a type of GOS.
A study conducted in the Department of Animal Science at
the University of Illinois showed that low-level supplementation of
scFOS, GOS and their combination have positive effects on select

WATTAgNet.com | November/December 2012

 | 13

The world´s top event for animal production

u s a l em
Jer ichoke
ar t

For new func-

tional benefits
L act obacillus
in petfoods,
prebiotics from
sources such as garlic, Jerusa-
lem artichokes, sugar beets and bananas can be combined with
probiotics like Lactobacilli or Bifidobacteria to create synbiotics.
Your Business
Driven by Innovations
SEE THE PRESENTATION
View Dr. K.B. Kore’s Petfood Forum Asia 2012 presentation,
“Prebiotics, Probiotics and Synbiotics as Functional Foods for 145,000 high qualified visitors,
Dogs,” at www.petfoodindustry.com/46136.html.
interested in new technology for
dairy, pig, poultry and aquaculture
indices of gut health in cats (K. Kanakupt et al., 2011). Until this
study, not much research was available on the prebiotic effects of More than 1,900 international
non-digestible oligosaccharides in cats, the authors said (www. exhibitors on 180,000 m2 exhibition
petfoodindustry.com/6763.html). floor space
Multiple probiotic types
In his presentation, Dr. Kore described a probiotic as a “prepa-
The complete programme for animal
ration or product containing viable, defined microorganisms in production – breeding, feeding,
sufficient numbers that alter the microflora of the intestine and by housing, processing and marketing
that exert beneficial health effects on the host.” Examples of pro-
biotics commonly used in both human foods and petfoods include
Leading technologies for renewable
Lactobacilli, Bifidobacteria, gram-positive cocci, yeast and fungi.
The Probiotic Live dog food line, as its name implies, includes energies and decentralized energy
actual live probiotics in an extruded dry petfood, a first accord- supply
ing to the manufacturer, Bacterfield Corp. Though the company
(based in Luxembourg) doesn’t reveal its exact manufacturing
technology, it says the formula infuses the bacteria inside the kib-
ble, helping it remain stable for at least 15 months and allowing for Hanover / Germany
a dosage of 1 billion live Enterococcus faecium. The formulation
also includes prebiotics such as FOS. 13 – 16 November 2012
Because it’s often difficult to get probiotics to colonize and re-
main that way in the intestine, using them with prebiotics increases
Tel.: +49 69/24788-265, E-Mail: expo@dlg.org
persistence in the GI tract by providing a preferred food for the
probiotics, thus promoting their growth, according to Trevor Faber
of the University of Illinois (www.petfoodindustry.com/7173.html). including

November/December 2012 | WATTAgNet.com www.eurotier.com

 14 |
Maximize Figure 2. Comparative count of
Petfood ingredientsbacteria
health-positive

your process
Dogs fed a synbiotic (combination of prebiotics and probiotics)
figure
had 2: Comparative
the highest count
count of health-positive of (fecal Lactobacilli
bacteria
health-positive
and bacteria
Bifidobacteria) in the study by Dr. Kore and colleagues.

11
with lowest operational costs 10
9

Count (log10cfu/g)
8
7
6
5
4

10.12
8.17
8.80

9.09

9.48

9.24
9.78

9.90
3
2
MICRO DOSING
UNITS Lactobacilli Bifidobacteria

• Wide dosing capacity range ■ Control ■ Prebiotic ■ Probiotic ■ Symbiotic

from 1 to 200 kg
Source: Dr. K. B. Kore
• Durable construction and
low maintenance Dogs fed a synbiotic (combination of prebiotics and probiotics)
had the highest count of health-positive bacteria (fecal Lactoba-
• Special shaped cilli and Bifidobacteria) in the study by Dr. Kore and colleagues.
dosing slides
for fine
and coarse
dosing Mixing prebiotics and probiotics
Recent research into these combinations is producing ingredients
• Minimum called synbiotics, Dr. Kore said. He defined a synbiotic as a mixture of
contamination
of product prebiotics and probiotics that helps improve survival and implantation
of live microbes in the GI tract by selectively stimulating the growth
and/or activating the metabolism of one or a limited number of health-
promoting bacteria. Examples of synbiotics include:
▶ Bifidobacteria + FOS ▶ Lactobacillus + FOS
▶ Lactobacillus + inulins ▶ Bifidobacteria + inulins
Dr. Kore shared results of a study he conducted with A.K.
Pattanaik and K. Sharma of the Centre of Advanced Faculty
Training in Animal Nutrition at the Indian Veterinary Research
Institute. To evaluate the functional food properties of prebiotics,
probiotics and synbiotics, they randomly divided 16 Labrador
Retrievers into a control group and three treatment groups: one
receiving a prebiotic (chicory inulin at 1 percent of diet on a dry
matter basis), one receiving probiotics (at 5 percent of diet, provid-
Visit us at IPE Atlanta ing 1×109 of L. acidophilus) and one receiving synbiotics (a combi-
Stand CBH International nation of the prebiotic and probiotic).
Hall B - nr. 5471 The results revealed no effect on nutrient intake, but digest-
ibility was significantly lower in the prebiotic group, digestibility of
protein was higher in the synbiotic group and digestibility of fat,
fiber and calcium was higher in both the probiotic and symbiotic
groups. The researchers concluded:
▶ Prebiotics positively modified fiber digestion, calcium absorp-
tion, hindgut health indices and intestinal microflora;
▶ Probiotics improved some nutrient utilization (fat, fiber, calcium),
hindgut health attributes and intestinal microbial balance; and
w w w. a a r s e n . co m
WATTAgNet.com | November/December 2012
 | 15

Research applications for

petfood manufacturers 
Research to date on prebiotics, probiotics and synbiot-
ics has shown health benefits for pets and been applied
in the marketplace. K.B. Kore, PhD, assistant professor
at Navsari Agricultural University in India, believes future
research and development by petfood manufacturers and
suppliers could include:
▶ Finding new natural sources of prebiotics;
▶ Selecting new and more specific strains of probiotics for
health and well-being of the host (age group, health of the
population, disease specific);
▶ Developing technology for non-dairy probiotics (grain-
based materials);
▶ Creating appropriate delivery vehicles for synbiotics; and
▶ Developing products with sensory properties, viability
during processing and stability in the product as well as
Figure 3. Comparative count of
during storage.
health-negative bacteria
The synbiotic group (dogs fed a combination of prebiotics and
figurehad
probiotics) 3: Comparative count
fewer health-negative of (fecal Coliform and
bacteria
Clostridia than the other groups in the study by Dr. Kore.
health-negative bacteria
11
10
9
Count (log10cfu/g)

8
7
6
5
4
7.01
6.36

5.81

5.61

9.46
9.13

8.82

8.38

3
2
Coliforms Clostridia
Visit us in Hannover!
■ Control ■ Prebiotic ■ Probiotic ■ Symbiotic
Source: Dr. K. B. Kore
Hall 15 booth D11
The synbiotic group (dogs fed a combination of prebiotics and
probiotics) had fewer heath-negative bacteria (fecal Coliform and
Clostridia) than the other groups in the study by Dr. Kore.

▶ Synbiotics showed added or synergistic effects over using

pre- or probiotics alone in all aspects of functional properties
ascribed to prebiotics and/or probiotics.
Dr. Kore said future research should continue to search for alter-
nate sources of these functional ingredients and target the influence
of proven synbiotic combinations across different feeding regimens,
breeds and lifestyles. Synbiotics could also aid in nutrigenomics,
exploring the functional role of the food at the genetic level.

November/December 2012 | WATTAgNet.com

16 |

Calf nutrition by Emmanuelle Apper, Ph.D., and Frédérique Respondek, M.Sc.

Improving veal calf growth

with soluble fibers
Supplementing veal calves’ diets with short-chain fructo-oligosaccharides
can potentially offer benefits to both feed producers and breeders.

A number of tests have been conducted
regarding short-chain fructo-oligosac-
charides over the past 20 years, and the
conclusion is clear: dietary supplements
of short-chain fructo-oligosaccharides for
calves result in higher performance. This
information, if implemented, could result
in substantial economic benefits for both
feed manufacturers and breeders.
Short-chain fructo-oligosaccharides
are a combination of a terminal glucose
molecule and one, two or three molecules
of fructose. They naturally exist in many
plants but can also be produced from
beet sugar by enzymatic process. In this
case, the composition is stable among
the three following molecules: GF2: 37
percent, GF3: 53 percent, and GF4: 10
Tests have shown that dietary sup-
plements of short-chain fructo-oligo-
saccharides for calves can result in
higher performance.
percent. They are listed in the European
catalog of feed materials as Fructo-
oligosaccharides (number 4.1.14).
Reducing pathogenic bacteria
Not digested in the small intestine,
these carbohydrates are considered a
source of soluble fibers that can present
some prebiotic properties (Gibson et al.,
2004). In many animal species, short-chain
fructo-oligosaccharides are known to par-
ticularly stimulate the growth of intestinal
bifidobacteria and some strains of lactoba-
cilli (Xu et al., 2003; Tzortzis et al., 2004).
In veal calves, short-chain fructo-oligo-
saccharides have been shown to modulate
the intestinal microbiota and reduce the
prevalence of potentially pathogenic bac-
teria. As an example, dietary supplementa-
tion of short-chain fructo-oligosaccharides
increased the level of bifidobacteria
measured in faeces (Bunce et al., 1995).
Furthermore, a recent in-vitro study dem-
onstrated that the microbiota inhabiting the
terminal part of the ileum was able to fer-
ment these molecules. In comparison to a
control fermentation substrate, the medium
containing short-chain fructo-oligosac-
charides induced a greater development
of lactobacilli and streptococci as well as
lactate-utilizing bacteria, while there was
no difference on clostridium perfringens
(Philippeau et al., 2010).
It was noticeable that the stimulation
was negatively correlated to the initial level
of bacteria; the lower the initial concentra-
tion, the higher the stimulation. Due to their
fermentation by lactic acid bacteria, there
was an increase in L-lactate production but
very limited increase of D-lactate produc-
tion accompanied by a higher concentra-
tion of acetate and butyrate than with the
control medium. This is fully in accordance
with what was observed in vivo in other
animal species like horses (Respondek et
al., 2008a).
Improved weight gain
Several studies have demonstrated that
the addition of 2 g to 6 g of short-chain

WATTAgNet.com | November/December 2012

 | 17

figure 1: Feed conversion

Figure 1: Feed conversion
of short-chain fructo-oligosaccharides was
ratio loss* reported (1992, unpublished Beghin-Meiji).
ratio loss* Figure 1 shows that growth performance
0.00
increases as soon as 2 g/d/calf, and
reaches a threshold when about 3 g/d/
calf of short-chain fructo-oligosaccharides
are fed. Maximum feed conversion ratio
-0.02
is reached when 3 to 6 g/d/calf of short-
chain fructo-oligosaccharides are added.
Stronger effects in case of stress 
The ability of short-chain fructo-oligosac-
charides to promote growth performance are
more pronounced when animals are under
stress conditions. This point is important to
underline since young animals are very sen-

-0.04

-0.06
0 2 4 6
scFOS, g/d/calf
*Data came from four trials where calves were not
submitted to a stress. No diet was supplemented with
antibiotics.Experimental period lasted from 42 to
142 d and body weights of calves were between
45 and 145 kg at the beginning of the trial.
Source: unpublished Beghin-Meiji 1992, 2001
and Grand et al., submitted for publication.

Figure 1: The effect of short-chain fructo-

oligosaccharides on the feed conversion
ratio for growing and finishing calves,
expressed as a unit of loss, compared to
the control diet containing no short-chain
fructo-oligosaccharides.

fructo-oligosaccharides per day improved

body weight gain (+1 to 4 percent com-
pared to the control diet, with no short-
chain fructo-oligosaccharides) and the
feed conversion ratio (Figure 1) of calves
that weighed between 45 kg and 140 Butyrate for gut health bio-regulation:
kg at the beginning of the experiments. we deliver to where it is really effective!
Furthermore, the addition of short-chain
fructo-oligosaccharides in diets contrib-
uted to homogenized growth performance
ADIMIX® 30 COATED
of calves. spray-cooled & encapsulated butyrate
In a 2011 study conducted in France,
an addition of 0.15 percent and 0.30 per- Its unique precision delivery system guarantees the desired effect
cent of short-chain fructo-oligosaccharides in the small and large intestine. Adimix:
in diets only increased growth perfor- • Enables a stable intestinal flora
mance a small amount (1 percent on • Supports the structural development of the intestinal tract
average), but strongly reduced standard • Stimulates enzyme secretion and immunity
deviation of the body weight of calves at • Improves biological functions at the cellular level
slaughtering. The increase of growth per-
formance was accompanied by a lower
Performance Enhancement
feed conversion ratio. As an example,
a feed conversion ratio of 1.55 for the
control diet without short-chain fructo-
www.nutriad.com
oligosaccharides dropped to 1.46 for the
same diet after an addition of 0.50 percent

November/December 2012 | WATTAgNet.com

 18 |
Veal calf growth
RELATED STORY:

Stay in sitive to stress situations like weaning.

In the case of a nutritional stress,
Optimizing dairy cow production,
www.WATTAgNet.com/24029.html 

the loop.
Kaufhold et al. (2000) evaluated the effects
of short-chain fructo-oligosaccharides
on the growth performance of calves fed
with both normal and high levels of lactose
in their diets. They noted in both cases
A resource an increase of growth performance but
reported that all effects observed were
for FEED prominent after lactose loads.
In the same way, in a recent study (Grand
et al., submitted for publication), a dietary
addition of 3 or 6 g/d of short-chain fructo-
oligosaccharides increased the carcass
weight of calves by 3.9 kg.
Other effects of short-chain fructo-oligo-

transportation In other experimental conditions, and no-

tably with a weaning stress, Tai et al. (2009)
saccharides are expected and are actually
studied in calves. Thus, further research is
efficiencies... reported a very strong improvement and a
homogenization of
currently underway to evaluate the interest
of adding short-chain
growth performance fructo-oligosaccha-
...delivered of early-weaned Studies have shown rides for prevention of
calves by addition of glucose intolerance
directly to short-chain fructo-oli- that short-chain generally appearing
gosaccharides. In this in veal calves fed high
your inbox. study, calves weaned fructo-oligosaccharides amounts of simple
at only 40 days old sugars, as it has
instead of 70 days can improve already been seen in
and supplemented horses and in dogs
with 2 to 6 g/d of weight gain. that short-chain fruc-
short-chain fructo- to-oligosaccharides
oligosaccharides for can slightly improve
30 days reached the same body weight gain metabolic parameters in such cases
than those of calves weaned later (520 g/d (Respondek et al., 2008b; Respondek et
on average). In contrast, the body weight al., 2011).
gain of non-supplemented early-weaned
calves averaged 371 g/d. Recommendation
Finally, probably due to the effects on
Improved carcass classification intestinal health and metabolism and to
A French study (2001, unpublished the change in intestinal microbiota, addi-

SideroadS
oad
Engineered Transportation Efficiency
Beghin-Meiji) first showed that carcass
weight was increased by 1.2 percent.
Secondly, the study demonstrated that in
the European system, carcasses were less
classified as P when calves were fed a diet
supplemented with 0.15 percent of short-
chain fructo-oligosaccharides than with
a control diet containing no short-chain
Reserve your fructo-oligosaccharides. These results put
digital copy today! forward a better carcass conformation
Visit http://eforms. when calves were fed with short-chain
kmpsgroup.com/wattpub/ fructo-oligosaccharides.
forms/side_subscribe.htm Again, short-chain fructo-oligosac-
charides supplementation not only im-
proved carcass weights, but also tended
to homogenize this result since standard
Brought deviation strongly decreased from 9.1 per-
to you by cent for the control to 5.8 percent for the
short-chain fructo-oligosaccharides diet.
tion of short-chain fructo-oligosaccharides
increases growth performance, improves
carcass characteristics and allows homog-
enizing these criterions inside a group of
calves. These assumptions are particularly
true when calves are submitted to a stress.
In short, the addition of 3 to 6 g/d/calf
of short-chain fructo-oligosaccharides
in diets (or 1.5 to 3.0 kg/t of CMR) are a
good recommendation to optimize calf
performances.
Emmanuelle Apper is an animal nutri-
tion scientist for Tereos Syral. Frédéri-
que Respondek is the scientific and
regulatory affairs manager for Tereos
Syral. Both can be reached at regula-
toryscientific-syral@tereos.com.

WATTAgNet.com | November/December 2012

20 |
Nutrition by Bernardo Suárez and Arno van der Aa
in poultry feed from a cost perspective
Historically, betaine
has been used in
poultry feed in its
anhydrous form,
extracted from beets.
However, there may
be a smarter and less-
expensive choice.
Betaine has long been a known functional
nutrient in broiler nutrition. In the past, it
was mainly used in poultry feed as betaine
anhydrous, extracted from sugar beets;
but, now it is available as betaine hydro-
chloride, which is synthetically produced.
Recent research has shown that nutritional
properties of these products are equal,
unveiling a cheaper, non-hygroscopic and
year-round source of betaine for the feed
industry. However, special care should be
taken that the free-flowing properties of
betaine hydrochloride are always ensured,
since hygroscopicity may limit application
in feed plants. With a strong focus on the
crystallization process and the correct
application of a free-flowing carrier, a non-
hygroscopic betaine hydrochloride can be
produced.
Absorption and metabolism
Betaine is absorbed via duodenum.
Human studies showed rapid absorption
and distribution with a peak increase in
serum one to two hours post food intake.
Weigand and Kirchgessner (1981) reported
that betaine is absorbed in the gastroin-
testinal tract, where up to three-fourths
of it could remain at the intracellular level.
Intracellular accumulation takes place via
active (Na+ or Cl-) and passive (Na+) trans-
port systems.
Betaine is eliminated by metabolism,
not by excretion, and catabolized via a
series of enzymatic reactions that occur
in the mitochondria of liver and kidney
cells. The principal physiologic role of
betaine is to act as an osmolyte and as
a methyl donor. As an osmolyte, betaine
increases intracellular water retention
and, therefore, protects intracellular
enzymes against osmotic-induced inac-
tivation. As a methyl donor, betaine par-
ticipates in the methionine cycle (mainly
in the liver) and can be further used in
transmethylation reactions (Figure 1) for
synthesis of essential substances like
carnitine and creatine (Kidd et al., 1997).
Biological equivalence
Betaine is a zwitterion metabolite,
also known as trimethylglycine. It was
first discovered in sugar beets, but it
is also present in other plants, animals
and seafood. However, sugar beets
contain exceptionally high levels of be-
taine, which accumulate as a condensed
soluble (approximately 116,000 mg/kg;
Eklund et al., 2005). Currently betaine is
available in several purified forms (anhy-
drous, monophosphate and hydrochlo-
ride betaine).
Questions have been raised asking
if osmoregulatory properties of betaine
hydrochloride are similar to those of be-
taine anhydrous. To study this question,
an in vitro trial was set up to mimic gastric
passage. De Krimpe (University of Ghent,
2010; unpublished) evaluated the biological
equivalence of different betaine sources
(monohydrate and anhydrous, produced
by extraction, versus betaine hydrochloride
and anhydrous, produced by chemical
synthesis). The products were dissolved in
a water and hydrochloride solution with pH
2.3 (gastric conditions) and then analyzed
using high-performance liquid chromatog-
raphy – electrospray ionization mass spec-
trometric and direct mass spectrometric
analysis.
Results showed that irrespective of the
ionic form and production method (natural
extraction versus chemical synthesis), dif-
ferent sources of betaine gave the same
analytical results (same m/z retention time
pairs). Therefore, no difference in biological
activity or osmoregulatory function should
be expected. If, after gastric passage, both
molecules are identical, no differences be-
tween betaine hydrochloride and betaine
anhydrous as an effective feed additive
could reasonably be expected.
WATTAgNet.com | November/December 2012
 | 21

FIGURE 1: Transmethylation reactions Dietary choline, betaine and methionine

are interchangeable for transmethylation.
DNA Possible increased
Folate (diet) Methylation atherogenesis
Methionine
(diet) percent of the supplemental methionine
MTHFR S-adenosyl
S-adenosylmethionine can be substituted by betaine without
homocysteine
FOLATE Choline negative effects on performance. A more
(diet) conservative replacement approach
METHIONINE
was evaluated in popular magazines
Vitamin by Lensing and Van der Klis (2007) and
Methylene-THF CHOLINE
B12 Cresswell (2010). Both experiments stud-
BHMT Betaine ied the bioequivalency between betaine
Methyl-THF (diet) and choline/methionine in broilers diets;
BETAINE whereas choline was fully replaced, me-
Vitamin B6
thionine decreased by 25 percent to 30
Cysteine
HOMOCYSTEINE percent of daily requirements. Within this
range of replacement, no differences in
broilers performance were observed.

Betaine in poultry production in poultry. Pesti et al., (1979) showed Practical studies
Elklund et al., (2005) and Ratriyanto that the dietary addition of betaine and In line with the results found by
et al., (2009) carried out comprehensive methionine can replace each other in Cresswell, Dr. Rama Rao (2011, unpub-
reviews of the effects of feeding betaine broiler chicks. Florou-Paneri et al., (1997) lished) performed a trial at IPME in Pune,
to poultry. Results of nutrient digestibility, showed that between 30 percent and 80 India, and showed improved performance
animal performance, metabolism and
improvement in carcass leanness were re-
viewed and reported. These peer-reviewed FIGURE 2: Trial results Excential Beta-key on production
papers illustrated the benefits of betaine performance, slaughter yields and economical evaluation
as a feed additive capable of improving
Excential Beta-Key Treatments T1 (Control) T2 T3 T4
animal performance and slaughter charac-
Final Body Weight (kg) 2.11 2.16 2.12 2.15
teristics. Studies included in these reviews
Performance
were conducted with a particular scientific
thought, and animal responses were the Feed Conversion (kg feed/kg growth) 1.92 1.83 1.89 1.85
result of one of the betaine’s modes of Economical evaluation
action. These were both influenced by the Average feed cost (USD/kg) 0.368 0.370 0.367 0.368
concentration of other methyl donors in the Average feed consumption (kg) 4.04 3.96 4.07 3.98
diet and the presence of either an osmotic Total feeding cost (USD/bird) 1.487 1.466 1.492 1.464
or metabolic stress. Chick cost (USD/bird) 0.56 0.56 0.56 0.56
Medication/vaccination (per bird) 0.05 0.05 0.05 0.05
Methyl donor and sparing effect Administrative (per bird) 0.11 0.11 0.11 0.11
Dietary supplementation of betaine Total production cost (USD/bird) 2.207 2.186 2.212 2.184
may reduce the requirement of other Average live body weight (kg) 2.11 2.16 2.13 2.15
methyl donors, such as methionine Average dressing % 74.82 77.60 75.99 77.57
and choline (Siljander-Rasi et al., 2003). Average dressing weight 1.577 1.679 1.616 1.671
However, this theoretical application Production cost per kg live weight (USD) 1.05 1.01 1.04 1.01
must be subjected to considerable Cost of production
1.40 1.30 1.37 1.31
analysis before practical implementation per kg dressed weight (USD)
(Matthews et al., 2001; Zhan et al., 2006). T1: Control diet
This sparing effect of methionine and
T2: Diet containing Excential Beta-key @ 500gm per/MT
choline has been thoroughly investigated
T3: Diet containing Excential Beta-key @ 500gm per/MT by
replacing 750gm CholineChloride & 200gm Methionine
A trial at IPME in Pune, India, showed T4: Diet containing Excential Beta-key @ 1000gm per/MT by
improved performance and carcass replacing 1000gm CholineChloride & 300gm Methionine
yield for all treatments. Purities: Excential Beta-key: min. 71%; CholineChloride 75%; DL-Methionine 98%

November/December 2012 | WATTAgNet.com

22 |
Reconsidering betaine
Figurehydrochloride
figures 3 & 4: Betaine 3 & 4: Betainealleviate
hydrochloride
the negative
and panting heat stress
(adapted on to
from rectal temperature
Haldar et al, 2011) and panting (adapted from to Haldar et al, 2011)
42.2
42.1
CP
42
Rectal temp. (ºC)
41.9 CN
41.8
41.7 Bet. 1.3
41.6
41.5
41.4
41.3
14 21 28
Days
It is clearly indicated that during heat stress, betaine hydrochloride relieves negative effects.
and carcass yield for all treatments.
Moreover, this trial presented an insight in
different application strategies. Two thou-
sand Cobb broiler birds were divided in
four treatments with two replicates each.
The control diets were typical corn-soy
diets containing 2,000 ppm, 1,500 ppm
and 1,500 ppm added choline chloride
(75 percent), as well as 0.61 percent, 0.58
percent and 0.45 percent total methionine
for starter, grower and finisher diets, re-
spectively. Best economical results were
achieved with on-top addition of betaine
(treatment two); an investment of $0.002/
kg feed resulted in 7 percent savings
in production costs of dressed weight.
Replacement of certain levels of choline
and methionine showed good economical
results. Treatment three (strong replace-
ment of choline and methionine) showed
that with lower feeding costs, a small
improvement in production could be
achieved. Treatment four (careful replace-
ment of choline and methionine) was a safe
strategy with equal costs of the diet, but a
good return on investment. Results of this
trial are summarized in Figure 2.
Osmoprotective properties  Several scientific publications have
The regulation of cellular hydration
state, and therefore cell volume, is im-
portant for maintenance of cell function
and several metabolic pathways; that
is, protein turnover and amino acid car-
bohydrate. Cells try to adapt to external
osmotic stress by accumulating inorganic
alleviate
the negative
impact impact
of heat stress on of
rectal temperature
Respiration rate (breaths/min.)
Bet. 2.0
35
ions (Na+, K+, Cl-) and organic osmolytes
(methylated amines and certain amino ac-
ids). However, the increase in intracellular
concentrations of inorganic ions is limited,
due to their destabilizing effect on protein
structure and enzyme function. On the
other hand, organic osmolytes can reach
high intracellular concentrations without
disturbing cellular functions. Betaine is
Currently betaine is
available in several
purified forms.
considered the most effective organic
osmolyte. It accumulates in gastrointesti-
nal tract cells regulating water flux across
the intestinal epithelium. Betaine had also
shown to inhibit cellular apoptosis and to
reduce energy expenditure for gastroin-
testinal tract cells.
Overcoming heat stress  of methionine, choline and energy as in
shown proof that betaine anhydrate can be
used to overcome heat stress. Attia et al.,
(2009) showed that the impact of severe
heat stress could partially be overcome by
adding betaine to the diet in slow-growing
broilers. Adding 1 kilogram betaine to
the diet improved weight gain and feed
70
68 CN
66 CP
64
Bet. 1.3
62
60
Bet. 2.0
58
14 21 28 35
Days
conversion, compared to negative control
treatment. More importantly, rectal tem-
perature decreased (43.2 C versus 41.9
C) compared to negative control. Panting,
a mechanism of heavy breathing to lose
heat via evaporation, was also reduced
from 78.3 breaths/minute to 63.9 breaths/
minute.
Haldar et al., (2011) presented a study
under more practical conditions showing
that under (milder) heat stress conditions
(31 C, ±85 percent relative humidity) in
conventional broilers, the same effects
could be expected. More importantly,
they showed that these results could be
obtained using betaine hydrochloride and,
thereby, gave practical proof that betaine
hydrochloride has similar osmoregula-
tory properties as an anhydrous source.
Heat stress started at day 14. In this trial,
a positive control and negative control
were used, whereby the negative control
treatment was lowered in methionine (850
gr/MT), added choline was removed and
energy was reduced (-50 kcal ME). Betaine
hydrochloride was added at 1.3 kg/MT and
2.0 kg/MT, whereby the same replacement
negative control was used. Body weight of
broilers at 38 days increased from 1.80 kg
(control) to 1.84 kg and 1.86 kg for birds
fed 1.3 kg/MT and 2.0 kg/MT of betaine
hydrochloride, respectively. Feed conver-
sion improved from 1.67 in positive control
to 1.63 (1.3 kg betaine HCl) and 1.62 (2.0
kg betaine HCl).  Figures 3 and 4 show the
WATTAgNet.com | November/December 2012
 | 23

Related story: 
Lowering poultry feed formulation costs
with phytogenics,
www.WATTAgNet.com/151320.html 

details of rectal temperature and panting,

clearly indicating that, during heat stress,
betaine hydrochloride alleviates these
negative effects.

Conclusions 
Betaine has been used in broiler nutri-
tion for many years. Scientific proof has
shown that betaine improves production
performance, replaces other methyl group
donors such as choline and methionine, as-
sists birds during heat stress and improves
slaughter characteristics. Many of these
WHO CARES...
published articles were not clear on the Who cares that one extra egg, one extra lactation,
source of betaine used (natural, extracted
betaine anhydrous or synthetic betaine hy- or even one extra pound of weight gain may
drochloride), and concern has been raised if ����������������������������������������������
betaine hydrochloride would be as effective thousands of farmers this year?
in osmoregulation as betaine anhydrous
from sugar beet extraction. The data pre- Alltech does!
sented above clearly show that if a proper
crystalline betaine hydrochloride is used, its In fact, so do the 2,800 people worldwide who
molecular structure is similar after gastric
passage as betaine anhydrous. produce Alltech’s range of patented, natural feed
Care should be taken that the product ������������������������������������������������
has good free-flowing properties and that �������������������������
it is non-hygroscopic. Practical applica-
tion trials with birds fed betaine during ��������������������������������������������
heat stress clearly showed the expected
improvement and therewith the mode ��������������������������������������������������
of action of betaine hydrochloride as an ����������������������������������
osmoprotectant.
This new form of betaine (betaine hydro- �������������������������������������������
chloride) has increased year-round availabil- animal health and performance through
ity since production is independent of sugar
beet production. Additionally, as the cost improved nutrition …naturally.
of betaine hydrochloride is generally lower
than that of betaine anhydrous, the applica- So the next time you ask the question, who cares?
tions of betaine in broiler nutrition should be ���������
reconsidered by feed producers.

Bernardo Suárez is global technical

manager and Arno van der Aa is busi-
DOES!
ness development manager, Excen-
tials BV (an Orffa Company). Alltech.com | /AlltechNaturally | @Alltech

Copyright, ©, 2012 Alltech, (GR: 10213). All Rights Reserved.

November/December 2012 | WATTAgNet.com
24 |

Additives by Teresa Ribeiro, Ph.D.

Using enzymes in poultry feed

to increase profits
While alternative Table 1: Specific enzymes used to supplement
specific cereal-based diets
ingredients are an
Enzymes β-glucanases Xylanases β-galactosidades
economical option Barley Wheat Grain legumes

for feed producers, Cereals Oat Rye

Triticale
Lupinus

nutritive value Enzymatic supplementation can improve the nutritive value of cereals containing high
levels of soluble non-starch polysaccharides.
can suffer in the
Table 2: Water-soluble NSP content of different cereals
process. Careful use
Cereal Wheat Triticale Oat Barley Rye
of enzymes may % Water soluble NSP 0.6 0.7 1.0 1.2 1.7

offer a solution. Different cereal grains have different non-starch polysaccharides content.

In an environment of high-cost corn,

many feed producers are choosing to
substitute corn with other crops that
have less nutritive value, such as wheat,
triticale, sorghum (low-tannin varieties) or
barley. However, these cereals are rich in
non-starch polysaccharides, and there is
a negative correlation between the diet
content in non-starch polysaccharides
and its nutritive value.
Enzymatic supplementation can im-
prove the nutritive value of cereals con-
taining high levels of soluble non-starch
polysaccharides. Several studies have
shown that enzymatic supplementation in
Why use an enzymatic
cocktail instead
of a specific enzyme
for a specific diet?
barley, wheat, triticale or sorghum-based
diets can enhance the animal’s perfor-
mance to a level compared with that ob-
tained in corn-based diets.
Commercially, these supplements are
characterized by being a cocktail with
different enzymes with different specifici-
ties. However, different cereals are rich
in different and specific non-starch poly-
saccharides. So why use an enzymatic
cocktail instead of a specific enzyme for a
specific diet?
Non-starch polysaccharides  An increase in digesta viscosity caus-
Non-starch polysaccharides are a
large variety of polysaccharide molecules,
comprising some of the most representa-
tive compounds of the cell wall. They can
be insoluble or soluble in water.
Insoluble non-starch polysaccharides
are indigestible, and in normal amounts,
they maintain the normal motility of the
gut. Soluble non-starch polysaccharides
are more susceptible to biological hydroly-
sis, especially in the last compartments
Related story:
Protease enzymes improve amino acid
digestibility
www.WATTAgNet.com/150026.html
of the birds’ gastrointestinal tract, such as
the caecum. Soluble non-starch polysac-
charides display an anti-nutritive effect
for poultry due to the resulting increase in
digesta viscosity.
es a reduction in digesta passage rate
and reduces feed intake and digestibility.
It also causes a modification in gut physi-
ology that results in an enlargement of
the GI tract. The lower passage rate also
results in the proliferation of a fermentative
anaerobic microflora in the upper com-
partments of the GI tract.
Digestion 
The normal and healthy microflora is

WATTAgNet.com | November/December 2012

26 |
Enzymes in poultry feed
composed of facultative anaerobic micro-
organisms in jejunum and strict anaerobic
microorganisms in caecum. The decrease
in digestibility is due to an increase in
size and stability of digesta layers without
motility in mucosa surface leading to a mi-
nor contact between feed and enzymes,
which leads to a decrease in nutrient
availability.
These consequences are more fre-
quent when the carbohydrate degree of
polymerization is higher. The digestion of
non-starch polysaccharides depends on
the animal (presence of microflora able
charides in the diet (anti-nutritive effects of Figure 1: Use of a single
non-starch polysaccharides are related to enzyme or an enzymatic
the concentration of them in the diet). mixture on barley-based diets
Enzyme supplementation 
As described above, there is a nega-
tive correlation between the diet content NC
in non-starch polysaccharides and its
nutritive value. To overcome this problem,
enzyme supplementation in diets based Commerical mixture
on cereals rich in non-starch polysac-
charides is widely used. The addition of
enzymes to monogastric animal diets
reduces the degree of polymerization of β-glucanase
non-starch polysaccharides, which then
have a lower capacity to affect digesta

0
0

0
to digest non-starch polysaccharides viscosity.

115
85

95
90

increases with animal age), the solubility
of non-starch polysaccharides, chemical
structure of polysaccharide (linkage be-
tween sugars determines the fermentation
extension of the different carbohydrates),
and the amount of non-starch polysac-
Using specific enzymes
makes it possible to
use cereals with lower
nutritive value.
Enzymes need only to cleave the
carbohydrate at a few places in the poly-
saccharide chain to greatly reduce the
viscosity of solutions and thus enhance
nutritive value. This supplementation
improves nutrient digestibility and feed
intake, increasing the animal’s perfor-
mance. The polysaccharide disruption
not only reduces the viscosity, but also
releases nutrients and thus increases the
feed metabolizable energy.
Barley and oats are rich in β-glucans,
which are responsible for the low nutri-
tive value of the diets based on these raw
materials. Feed supplementation with
β-glucanases decreases the polymeriza-
110
10
10
Body weight (g)
Body weight at 28 days of broilers fed on
barley-based diets non-supplemented
(NC) or supplemented with a single and
specific enzyme (β-glucanase) or with a
commercial mixture with a broad range
of activities.
tion degree of β-glucans, allowing a better
use of the nutrients released, and lead-
ing to an increase in feed intake and to
a decrease in the feed conversion ratio.
Arabinoxylans are the major contributors
to soluble non-starch polysaccharides

WATTAgNet.com | November/December 2012


FIGURE 2: Endogenous
enzymatic activity for barley
HA-
HA+
LA-
LA+
0
0
0
20
40
60
80
0
14
12
10
Body weight (g)
Body weight at 28 days of broilers fed
on barley-based diets displaying high
endogenous enzymatic activity (HA) or
low endogenous enzymatic activity (LA),
which were supplemented (+) or not (-)
with a commercial enzyme mixture.
released from wheat, rye and triticale. In
the same way, also xylanases can depo-
lymerize this substrate and improve its
nutritive value.
Advantages of single specific
enzymes
Several studies have shown equal or
better results in broilers’ performance
when a single specific enzyme is used
compared with a commercial mixture.
This suggests that it is possible to use
just one enzyme, which could save feed
producers money in enzyme supplemen-
tation. Besides the supplementation with
enzymes, it is also important to evaluate
the need of the supplementation. It has
been shown that endogenous enzymatic
activity is encountered in some crops,
such as barley.
Additionally, some study results have
shown that exogenous supplementation
is redundant when some barley varieties
are used. This redundancy was explained
by a high endogenous enzymatic activity
November/December 2012 | WATTAgNet.com
| 27
in barley crops that was shown to be sat-
isfactory for the improvement of nutritive
value of this cereal. This was evaluated by
the decrease in digesta viscosity and im-
provement in the animal’s performance.
Justifying enzymatic
supplementation
It seems that it is very important to
evaluate the endogenous activity of the
grains to justify the use of enzymatic
supplementation. It is also known that
• Fine grinding
different cereal grains have different non- • Microscaling
starch polysaccharides content. From low
to higher contents: rice, sorghum, corn, • Pelleting
wheat, triticale, rye and barley.
Non-starch polysaccharides content
• Steam flaking
also seems to be dependent on some
other factors, such as crop variety and
Get it all with CPM:
environmental growth conditions. Different Your Partner in Productivity
varieties of the same cereal may have dif-
ferent non-starch polysaccharides content
and, consequently, have a different impact
on animals’ performance. Using specific
enzymes in poultry nutrition makes it pos-
sible to use cereals with lower nutritive
value. Cereals with lower nutritive value
usually have lower prices, which may be an
opportunity for feed producers.
Enzyme engineering
can be part of a
solution to the feeding
problem in poultry.
Enzyme engineering can be part of a
solution to the feeding problem in poul-
try. Enzyme engineering based on most
efficient microorganisms, together with
the use of recombinant genetic manipu-
lation, can improve enzyme efficiency
and consequently can help increase the
widespread use of cereals as a corn al-
ternative.
Teresa Ribeiro is a post-doctoral
researcher on the faculty of Veteri-
nary Medicine (CIISA) at the Technical
University of Lisbon.
28 |
Product news
Feed Management
Systems Inc. Pennent
Mill Manager software
Feed Management Systems
Inc. of fe r s Pe nne nt Mill
Manager. Pennent Mill Manager
is a feed business operations
software solution that enables
broad data integration and ex-
ecution with mobile and cloud
capabilities.
www.feedsys.com
Purina Animal Nutrition
HeiferSmart nutritional
supplements
The HeiferSmart line of dairy
heifer nutritional supplements
from Purina Animal Nutrition
consists of HeiferSmart Protein
Tub, Booster Tub and Mineral.
These products were devel-
oped to address the nutritional
needs of heifers when they are
transitioned to forage and pas-
ture-based diets.  HeiferSmart
Booster Tubs and Mineral also
contain Availa 4 organic trace
minerals.   opening lid and a dual-speed
www.purinamills.com
Applegate
Feed Train ATV Feeder
Applegate offers the Feed Train
ATV Feeder as an alternative
to bucket feeding livestock in
remote areas. The ATV feeder
WATTAgNet.com | November/December 2012
supports many types of feed
(including cubes) and the feed
tank is translucent white plas-
tic. Features include a feed
capacity of 500 pounds, a
4-inch auger, an 18-by-28-inch
controller with 15-foot cord that
dispenses feed at two speeds.
www.aggrowth.com
Vortex Valves
HDPV2 slide gate
Vortex Valves’ HDPV2 slide
gate can be used in a variety of
applications for handling sticky
and/or abrasive powders. It fea-
tures a wide bonnet, long body
frame and replaceable nylon
insert on the rising clevis. The
valve’s design incorporates an
o-ring seal through the bonnet
 | 29
In Agrisolutions
Since 1953

compliant silicone in blue or

and utilizes reusable precut
gaskets to eliminate material
leakage, as well as incorporates
an air cylinder mount cast into
the frame.
www.vortexvalves.com
BlueLevel Technologies
Model DA disc aerator
M o d e l DA d i s c a e r a to r
from BlueLevel Technologies is
a 4-inch diameter aerator using
Food and Drug Administration-
white.  The hardware is con-
structed of zinc plated steel,
and the unit can be mounted
from the inside or outside of the
bin. Each Model DA disc aera-
tor can aerate an approximate
20- to 24-inch diameter area,
designed for periodic opera-
tion using higher 20-30 psi air
pressure compared to standard
rectangular pads.
www.bluelevel
technologies.com

Ne
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• Boosts animal immunity by promoting balance of
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enzymes
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For inquiries, please contact:

Tel: +65 6759 1811 . Fax: +65 6759 1855 Scan to find out more
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November/December 2012 | WATTAgNet.com

30 |
Products
KW Precision blends
Precision blends from KW are offered in
10 varieties for dairy, beef and youngstock.
Some formulas include Precision Coarse
Calf, which is designed to drive intakes for a
smooth weaning transition, while Precision
Youngstock contains rumen-bypass proteins
to maximize performance of young cat-
tle. KW Precision blends are available in load
sizes from 5t to 29t, either blown, tipped or
for self-collection.  The auto-tapper for pellet classifiers from
www.kwalternativefeeds.co.uk
Tolomatic ERD electric cylinders
Tolomatic’s ERD electric cylinders are
rod-style electric actuators designed as an
alternative to pneumatic cylinders. They are
available in four body sizes, with a reverse-
parallel motor mount and a rear clevis pivot
mount. The actuators can be built-to-order
in stroke lengths from 8 to 24 inches and
forces up to 500 pounds.  Bruker TANGO FT-NIR spectrometer
www.tolomatic.com
Witte auto-tapper
for pellet classifiers
Witte automatically frees off-spec pellets
lodged in the screening deck. It features
a pneumatic actuator set on an adjustable
cycle timer. The auto-tapper is available as
an option on the company’s entire line of
pellet classifiers and may be retrofit on Witte
classifiers in the field.
www.witte.com
TANGO from Bruker is a stand-alone,
Fourier Transform Near Infrared, FT-NIR
spectrometer. TANGO is a benchtop
system with touch-screen operation that
identifies and quantifies the constituents
in animal feed. TANGO is GMP- and 21
WATTAgNet.com | November/December 2012
 | 31

Avery Weigh-Tronix draft trains at speeds up to 15 mph (25

Streamline rail scale km/h). Featuring twin-strip technology,
The Avery Weigh-Tronix Streamline rail Streamline ignores horizontal and tor-
scale provides accurate, cost-efficient and sional deflections, only measuring the
quick-to-install train weighing. Streamline vertical forces of train wheels, absorbing
is installed directly onto the track without dimensional imperfections in the fixing
the need to cut or weld rails. Streamline method or the rail itself. 
weighs static or in-motion, full or two www.wtxweb.com
CFR Part 11-compliant for working in
regulated environments, and is available
in two versions: one for measuring solids
in reflection and one for measuring liquids
in transmission.
www.bruker.com

John Deere 1910 Commodity Air Cart

John Deere offers the 1910 Commodity
Air Cart with three tanks: 200-bushel front
and rear tanks, and a 150-bushel center
tank. The 550-bushel model has hydraulic
meter drives, variable-rate drive and hydraulic
calibration. Customers can choose between
a 10-inch auger or 12-inch conveyor for load-
ing product into the cart. 
www.JohnDeere.com

John Deere 9 Series round balers

John Deere 9 Series round balers, available
in 12 models, are designed to produce tight,
uniform size and shape bales for a variety of
forage and silage baling needs. Balers fea-
ture gull-wing doors, ISOBus electronics for
responsive baler/tractor operation, Diamond
chains, and large cams and bearings. 
www.JohnDeere.com

November/December 2012 | WATTAgNet.com

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Products
Cardinal Scale Mfg. Co.
Harvester series scales
Cardinal Scale Mfg. Co.’s
Harvester series precast con-
crete scales are fully elec-
tronic truck scales with fac-
tory-poured concrete decks
for above ground, low-profile
weighing. The NTEP-certified
scales have a 50-ton overall
capacity and 25-ton concen-
trated load capacity for most
models. Multiple 10-foot wide
modules can be linked together
and scales offer up to 105-foot
long platform sizes.
www.CardinalScale.com
Romer Labs FluoroQuant
Afla test kit
The Romer Labs FluoroQuant
Afla test kit is a direct fluoro-
metric test method for afla-
toxins that can be performed
in less than five minutes, and
quantification is done with the
FQ-Reader. It provides quan-
titative aflatoxin results from 1
to 5,000 ppb.
www.romerlabs.com
ACS Valves SD Series
rotary valves
The SD Series of rotary valves
from ACS Valves is designed to
provide increased bulk material
throughput efficiency in feed-
ing and metering applications
when compared to stand-
ard 8-vane rotary valves. The
construction of the SD Series
rotor has a larger pocket area
between the vanes of the ro-
tor; enabling greater pocket-fill
November/December 2012 | WATTAgNet.com
| 33
capacity. Calculated at 100
percent pocket-fill capacity,
an SD Series rotary valve can
move over 5 percent more
cubic feet/revolution of bulk
material than a comparable,
8-vane rotary valve.
www.acsvalves.com
Don't waste
anymore, use ...
POWERJET
Efficient energy
POWERJET is dedicated to
Elanco Animal Health
peacefully managing the transition
Pulmotil
Elanco Animal Health Pulmotil periods and to allocate the
is a bovine respiratory disease, maximum of “efficient energy”
BRD, treatment for groups of to the animals production needs.
cattle in the early stages of an
outbreak that provides 14 days Save energy, use POWERJET !
of sustained in-feed therapy,
a practice that reduces stress
associated with cattle handling.
Pulmotil expands BRD control
Hall 1
Stand C 6
beyond the chute by providing
another option for groups of 09
cattle not requiring a meta-
phylaxis treatment on arrival
or when labor resources are
limited. The FDA has approved
the combination feeding of
Pulmotil with Elanco Rumensin, Additives & difference
an efficiency-enhancing feed B.P. 394 - 56009 VANNES Cedex - FRANCE
additive. neovia@invivo-nsa.com - www.neovia-additives.com
www.elanco.us
34 |
Products
Hampel Animal Care Calf Tel Pen
Front with height-adjustable buckets
Hampel Animal Care offers the Calf Tel Pen
Front pen system with independent height
adjustable buckets. The Pen Front system
allows the bucket height to be adjusted inde-
pendent of the pent front, as well as features
a feed divider and wide door opening.  AB Vista Nitroshure
www.Calf-Tel.com
Fortress Interlocks amGard Pro
safety gate switch interlocks
Fortress Interlocks offers the amGard Pro line
of safety gate switch interlocks. The amGard
Pro line is forward and backward compatible
to integrate with existing installed interlocks. Larson Electronics LXflashlights.
They also feature emergency release options, com HAL-2X400MH hazardous area
stainless steel heads and anti-vibration parts.  light tower
www.fortressinterlocks.com The  HAL-2X400MH hazardous area light
tower from Larson Electronics LXflashlights.
com is designed to provide high-power illumi-
AB Vista’s Nitroshure, manufactured by nation with a pair of metal halide lamps and an
Balchem, is an encapsulated urea supple- aluminum tower. This adjustable-height tower
ment that serves as replacement for soya can illuminate areas up to 50,000 square feet
in the diet of lactating cows. Nitroshure, a and comes with 50 feet of SOOW cord fitted
slow-release supplement, provides a source with an ECP 1523 5-15P Class 1 Division 1
of nitrogen and protein.  explosion-proof, straight-blade plug.
www.abvista.com www.magnalight.com
MILL MANAGER
on Sivado
Enabling PE
PEoPlE,
PEoPlE,
PowEring
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Ering Profits
The First Center of Business Operations—
Enabling Integrated Data, Execution
and A More Profitable Bottom Line
AVAILABLE NOW
To learn more, visit: www.feedsys.com.
WATTAgNet.com | November/December 2012
 The live yeast that keeps its promises
Stimulates rumen metabolism
Improves roughage digestibility and feed
efficiency
Controls acidosis
Limits digestive disorders in young
animals
Increases farmers’ profitability: more
milk, better lactation persistence, better
growth in fattening animals

Tel.: +33 (0)320 81 61 00

Fax: +33 (0)320 99 94 82
E-mail: contactlfa@lesaffre.fr
www.yeast-science.com

Better digestion for

better feed efficiency
Add the power of Phytogenics to your diet:
• A unique blend of herbs, essential oils and
functional flavors
• Proven in science and practice
• Tailored to the animal’s needs

digestarom.biomin.net

Naturally ahead

November/December 2012 | WATTAgNet.com

36 |
Products
Mayer Maschinenbaugesellschaft
mbH SILOKING wireless control and
weighing system
SILOKING wireless control and weighing sys-
tem from Mayer Maschinenbaugesellschaft
mbH is designed for trailed feed mixers. This
Web-based feeding management software al-
lows farmers to manage individual loading and
unloading programs on the PC. The complete
SILOKING wireless FM package includes the
“Basic” and “Premium” terminals, the interface
to the feeding management software, a USB
stick and the machine box mounted in a
protected position at the feed mixer.
www.siloking.com
Today’s competition
is tough,
so you need
every advantage.
40 years experience in creating unique advantages
in nutrition and viability makes us the perfect
partner for your direct-fed microbial needs
Give us a call today to learn more.
800-894-5396
info@primalac.com • www.primalac.com
Hamlet Protein HP 800 Booster
Hamlet Protein HP 800 Booster is a protein
supplement for piglet feed that combines soya
with yeast.  The supplement is specifically
designed to boost feed intake in runts, weak-
lings, low-weight and early weaned piglets.
www.hamletprotein.com
Mayer Maschinenbaugesellschaft
mbH SILOKING TrailedLine Duo
Mayer Maschinenbaugesellschaft mbH of-
fers SILOKING TrailedLine Duo vertical feed
mixers to prepare mix rations for cattle and
other ruminants. Offered in capacities from
14 cubic meters to 30 cubic meters, the
mixers are equipped with a FlowPlus hop-
per ring to improve feed flow, a turbo flight
at the mixing auger and sensor technology
to prevent faulty control of the hydraulic two-
speed gearboxes.
www.siloking.com
AB Vista ReaShure
ReaShure from AB Vista is a feed additive
designed to improve cow health, milk pro-
duction and reproduction. This fully protected
choline product provides nutrition for cows
during the pre- and post-calving transition
period. 
www.abvista.com
Novus International Inc.
Sporulin direct-fed microbial
Novus International Inc. offers Sporulin
direct-fed microbial for poultry gut health.
Sporulin is a blend of three strains of Bacillus
subtilis.  This probiotic can survive harsh
conditions during feed pelleting and storage.
www.novusint.com
WATTAgNet.com | November/December 2012
Process technologies, plants, and
aftermarket service. Global supplies
for the animal feed industry
■ Leading technologies
■ Global presence
■ Local service

Together, we make a difference!

ANDRITZ FEED & BIOFUEL

Europe, Asia, and South America: andritz-fb@andritz.com
USA and Canada: andritz-fb.us@andritz.com www.andritz.com

Everything is bigger at Agromek

27—30 November 2012

More than 500 exhibitors · 237 approved product news · Professionel activities · Knowledge sharing

tractors and crop management livestock machinery energy consulting construction,

farming equipment roads and parks

November/December 2012 | WATTAgNet.com

38 |

Advertisers’ index Sales team FeedInternational Leader in Technology, Nutrition and Marketing

Company Page Company Page

AB Vista Feed F.lli Fragola SpA ..........26
Ingredients .....................9 Feed Management
Agromed Systems .......................34
Austria GmbH.............31 Geelen Counterflow BV 8
Agromek ......................37 Kemin Industries ...........3
Alltech Inc ...................23 Liptosa..........................15
Andritz Feed Merck-MSD AH ....... C1a MICHAEL FRANS WILLEM DINGDING
VAN DEN DRIES VAN BEEMEN LI
& Biofuel A/S .............37 Muyang Group ............C4 Tel: +31 79 323 0782 Tel: +31 344 653 442 Tel: +86 137 640 39062
BASF Ag .....................C2 NEOVIA Fax: +31 79 323 0783 Fax: +31 344 653 261 Fax: +86 21 54133676
driesmvd@xs4all.nl beemenfw@xs4all.nl dingdingli@vip.163.com
Beijing Smile feed Sci. In Vivo NSA ...............33
& Tech. Co., Ltd..........28 Nutriad International ...17
Biomin Holding Perten Instruments
GmbH..........................35 AB ...............................29
Borregaard Priya Chemicals ...........34
Lignotech Ltd..............30 S A S I Lesaffre ...........35
CJ CheilJedang Star-Labs Inc ................36
corporation ..................25 Sudenga Industries IncC3
CPM/Roskamp U S Poultry TINEKE PAM MARY GINNY
Champion .....................27 & Egg Assn .................32 VAN SPANJE BALLARD HARRIS STADEL
Darling Intl Inc ............19 Van Aarsen Intl BV .....14 Tel: +31 495 526 155 Tel: +1 815 966 5576 Tel: +1 815 980 5938 Tel: +1 815 966 5591
Fax: +31 495 525 126 Fax: +1 815 968 0941 Fax: +1 847 908 7551 Fax: +1 815 968 0941
Diamond V .....................5 Walinga Inc ..................18 tvanspanje@wattnet.net pballard@wattnet.net mharris@wattnet.net gstadel@wattnet.net
DLG Service GmbH ....13 Zagro Singapore
DPI Global ...................36 Pte Ltd .........................29

Market place RICE PROTEIN &

Visit us at IPPE - Stand 1623

*Mix Uniformity and Cross Contamination Tests

** Mark Vitamin, Mineral or Medicated Premixes RICE SWEETENERS
in Feeds Product from facility in Pakistan —
***Protected Sodium Selenite and Sodium Selenate Non GM Conventional and / or
Feed Additives Certified Organic
MICRO TRACERS TM
Tel: (314) 919-5045
1370 Van Dyke Ave. • San Francisco, CA 94124 Email: FAYYAZSAJ@aol.com
JH Biotech, a leader in chelation technology: E-mail: MICROTRACE@AOL.COM
See u
• Patented process sa
A Hall t Booth #3 Website: www.microtracers.com
• 25 years in biotechnology on IP 0
PE 20 54, Telephone: 1-415-822-1100 • Fax: 1-415-822-6615
• Advanced formulation techniques 13

High Performance
• Bio-Enzymes, Yeast-cellwall & Cultures
• Mineral Proteinates & Methionates • Garlic Extract Oil for Eggs of less cholesterol &
• Chromium and Selenium Yeasts more Omega 3
• Acidifier & Antioxidant • Nano-Vit. A, D3, E, C: Keep potency in the water
• Mold inhibitor • Nano-Selenium+Vit. E: Poultry & Dairy cow
• Odor control
• Bio-Elements for Quality Red Pork-less drip loss
• Bio-Mycotoxin Binder
Tel: 1-805-650-8933 Fax: 1-805-650-8942
• Bio-Vmix: Anti-heat stress of animals
info@jhbiotech.com www.jhbiotech.com
DISTRIBUTORS WANTED

In Agrisolutions
Since 1953 Tel: 626-821-0438 (USA)
Inquiry: amecobios@gmail.com • www.amecobios.com

Zagro provides solutions that are proven to
preserve the quality of premix and feed against
molds and oxidation spoilage.
WE BUY AND SELL USED FEED AND GRAIN EQUIPMENT
• Pellet Mills • Coolers • Dryers • Roller Mills
• Flakers • Cleaners • Sewing Lines • Mixers
• Legs • Dust Filters • Air Pumps • New Conex Extruders

Haltox Zacid • Replacement parts for Insta-Pro® Model 2000 & 2500 extruders
LARGE INVENTORY IN STOCK

Zagribind Zatox
1400 S. Spencer Rd., Newton, KS 67114
For inquiries, please contact: Ph: 316-283-9602 • Fax: 316-283-9584
Tel: +65 6759 1811 Fax: +65 6759 1855 E-mail: sales@continentalagra.com
sales@zagro.com www.zagro.com Web: www.continentalagra.com

WATTAgNet.com | November/December 2012