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BINE ATI VENIT LA

CATEDRA DE FIZIOPATOLOGIE!

Asist. Dr. ORASAN MEDA SANDRA

Vom parcurge in acest semestru 14


lucrari practice:
20% din ele .. 3 absente posibile, dar care
a) trebuie recuperate!
b) trebuie platite, daca nu aveti
motivare de la Decanat

NOTA FINALA

EXAMEN SCRIS .. 70%


EXAMEN PRACTIC .. 20%
ACTIVITATE DIN SEMESTRU ..10% =
o prezentare powerpoint de 10-15 slides, cu
durata: 10 minute, un subiect legat de cursuri sau
de lucrarile practice.
Puteti alege dintr-o lista de titluri posibile, care
va fi trimisa prin e-mail.

TITLURILE LUCRARILOR PRACTICE

Studiul actiunii agentilor chimici asupra organismului


Studiul actiunii agentilor fizici asupra organismului
Inflamatia si reactia febrila experimentala
Algoritm de diagnostic al tulburarilor hemostazei
(2 sedinte)
Algoritm de diagnostic al tulburarilor functiei cardiovasculare ( 3labs)
Algoritm de diagnostic al anemiilor
Algoritm de diagnostic al tulburarilor functiei
respiratorii
Algoritm de diagnostic al tulburarilor metabolismului
- lipidic
- glucidic
- proteic

Lucruri necesare
1) Carte
LUCRARI PRACTICE DE FIZIOPATOLOGIE
STUDENTI DE ANUL III Editia a III sub
redactia Prof Dr Luminita Plesca Manea
Tipografia UMF- 1998
2) Caiet / foi indosariate
- protocolul experimentului
- cazurile clinice
3) Vaori normale pentru investigatiile de
laborator

EXPERIMENTUL
O metoda des folosita in
fiziopatologie

IN VIVO: pe animale experimentale


IN VITRO: pe culturi de celule

PROTOCOLUL EXPERIMENTAL
1.
2.
3.
4.
5.
6.

Scop
Materiale
Metoda
Rezultate
Discutii
Concluzii

Inainte & dupa experiment


evaluam parametrii biologici
1. Frecventa respiratorie
2. Frecventa cardiaca
3. Tonusul muscular (Crescut, scazut, contractii,
frison)
4. Temperatura rectala
5. Pupile (mioza/midriaza)
- comportament
- stare de veghe/ somn
- culoarea tegumentului
- salivatie / secretie lacrimala

FACTORI CHIMICI

FIRST EXPERIMENT
1. TASK: Observation of the organisms response to
barbiturates
2. MATERIALS: RAT, i.m. phenobarbital 0,2g/kg body.
3. METHOD: before and after injection (after 20 min) record:
heart rate, respiratory rate, rectal temperature, muscular
tone, pupils diameter and reflexes.
4. RESULTS: sleepy/deep sleep, decreased heart, respiratory
rate, rectal temperature, muscle tone, reflexes of pupils
and miosis
5. DISCUTION: barbiturates = GABA receptor agonists and
inhibit the CNS from confusion to death (coma in two
steps)
6. CONCLUSION: Poisoning with barbiturates produces:
hipnotic, sedative, tranquillizer, anticonvulsive effect.

SECOND EXPERIMENT
1.
2.
3.

TASK: Observation of the organisms response to organophosphates


MATERIALS: RAT,s.c. neostigmine 0,01%, 1ml/100g body.
METHOD: before and after injection (after 10 min) record: heart rate,
respiratory rate, rectal temperature, muscular tone, pupils diameter and
reflexes. Atropine 0,1% is injected to prevent death.
4. RESULTS:
After ATROPINE:
M1 effects: nausea, dyspnoea, miosis
mydriasis
M2 effects: bradicardia, hypotension
tahycardia
M3 effects: sweating, salivation, shadding tears
hiposalivation
N effects: twitching, fasciculations
CNS: anxiety, convusions, coma
5. DISCUTION: organophosphates = causes accumulation of Acetylcholine in
the synapses and produces M si N effects
6. CONCLUSION: organophosphates are absorbed by skin, lung, gastrointestinal tract, distributed in the tissues, slowly eliminated by the liver.

THIRD EXPERIMENT
1.
2.
3.

TASK: Observation of the organisms response to ethanol


MATERIALS: Sobolan, i.p. 1ml/ 100g body weight, alcool etilic 33%
METHOD: Before and after 5-10min from the administration have
to be assessed: respiratory rate, heart rate, rectal temperature,
musculature tone, pupils diameter and pupils reflex.
4. RESULTS:
Uncoordinatedly movements and reduced muscular tone
Redness of the ears, tail and paws, Agitation/ sleep, Mydriasis
Rise of the respiratory and heart rate, rectal temperature
Mydriasis.
5. DISCUTION: Ethanol is a CNS depressant that decreases activity of
neurones. The mechanisms of action of alcohol on nervous tissues
are not fully understood because even modest doses
simultaneously change many neurotransmitters and increase the
fluidity of neuronal cell membranes.
6. CONCLUSION: Effects of alcohol on the body:Euphoria, Lethargy,
Confusion, Stupor, Coma

FACTORI FIZICI

FOURTH EXPERIMENT
1.
2.
-

TASK: Observation of the organisms response to hypothermia


MATERIALS: 2 RATS, at 4 degrees, for 30 minutes
TEST: i.m. phenobarbital 2%
plegomaxin 0,5% + Romergan
Flaxedyl 0.04% ( the temperature regulating system is blocked)
- CONTROL:
3. METHOD: before and after injection, record: heart rate, respiratory rate,
rectal temperature, muscular tone, skin color
4. RESULTS:
TEST: decreased heart rate, respiratory rate, rectal temperature, muscle
tone, cyanosis
CONTROL: tahycardia, shivering, normal rectal temperature, restlessness
5. DISCUTION: when the temperature regulating system is blocked or the
physiological mechanism is outrun, hypothermia appears due to low
environmental temperature.
6. CONCLUSION: Lowering of body temperature depresses organs and system
functions.

HYPOBARISMUS

FIFTH EXPERIMENT
1. TASK: Observation of the organisms response to
hypobarismus
2. MATERIALS: 1 RATS, in chamber with vacuum pump
3. METHOD: before and after , record: behavior, heart rate,
respiratory rate, muscular tone, skin color
4. RESULTS: restlessness, tahypneea, tachycardia(irregular),
cyanotic skin, reduced muscle tone.
5. DISCUTION: at altitude the hypoxia increases the respiratory
rate as compensatory and signs appear: mental fatigue,
headache, fatigue, convulsions, coma
6. CONCLUSION: the effects of hypobarismus lead to: hypoxic
hypoxia, decreases of gases solubility in the organisms
tissues, dilatation of natural cavities volumes.
Compensatory mechanisms: increased heart rhythm, respiratory
rate, cardiac debit, RBC mobilization from storage organs
(spleen contraction)