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Abstract
The sensory properties of fat in milk were examined by sensory descriptive analysis. To date, no single food additive has been
completely successful in mimicking the sensory properties of fat in milk. This experiment investigated the eects of various factors
and combinations thereof on sensory properties and perceived fattiness of milk, and compared them to the actual fat content (0.1;
1.3 and 3.5% fat milk was used). The other factors studied were the addition of thickener, whitener, cream aroma and homogenisation. Multivariate data analytical methods (Partial Least Squares Regression) were applied for analysis of the data. The three
former additional factors contributed signicantly to perceived fattiness of the milk, and homogenisation had a small but not signicant eect. It was shown that a combination of thickener, whitener and cream aroma in 0.1% fat milk was approximately successful in mimicking sensory properties of 1.3% fat milk. # 2001 Elsevier Science Ltd. All rights reserved.
Keywords: Milk; Fats; Lipids; Sensory analysis; Multivariate data analysis; Partial least square regression (PLSR); Fattiness perception; Fat substitutes; Thickening agent; Whitening agent; Cream aroma; Homogenisation
1. Introduction
The sensory properties of milk are inuenced by the
fat content in milk (Phillips, McGi, Barbano, & Lawless, 1995a; Tuorila, 1986). In order to produce milk
and milk-based drinks with salient sensory properties, it
is necessary to understand how the dierent sensory
modalities are aected by the fat content. This also will
lead to a better understanding of what the perceived
fattiness in milk is comprised of. Previous research has
shown that much of the sensory dierences between
non-fat and other types of milk are mainly in found
appearance, texture and mouthfeel (Phillips et al.,
1995a; Tuorila, 1986). Various food additives have been
examined as a substitute of fat in milk. Phillips, McGi,
Barbano, and Lawless (1995b) observed some eect of
protein content on the sensory properties. The addition
of 2% non-fat dry milk to skimmed milk gave a similar
eect on the relative physical viscosity (measured by
capillary viscometer) as addition of 2% fat. Still, the
perceived texture (Thickness, Mouthcoating and Residual
0950-3293/01/$ - see front matter # 2001 Elsevier Science Ltd. All rights reserved.
PII: S0950-3293(01)00018-0
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Table 1
Factors varied in the experiment; levels and origin of material used
Experimental factor
Value of levels
Fat content
0.1% (w/w)
1.3% (w/w)
3.5% (w/w)
0
1 g/l
0
1 g/l
0
0.75 g/l
0
150 bar
Thickener
Whitener
Aroma
Homogenisation
Table 2
Sensory descriptors, their denitions and original words in Danish
Descriptors
Aroma
Creamy aroma
Boiled milk aroma
Lugt
Fldeagtig lugt
Kogt mlk lugt
Appearance/colour
Whiteness
Yellowness
Blueness
Transparency
Glass coating
Thickness visual
Udseende
Hvidhed
Gullighed
Blalighed
Gennemsigtighed
Glasvedhftning
Tykhed/viskositet
Flavour/Tastea
Creamy avour
Boiled milk avour
Sweet taste
Smag
Fldeagtig smag
Kogt mlk smag
Sd smag
Texture/Mouthfeel
Thickness oral
Creaminess oral
Residual mouth ll
Konsistens
Tykhed/viskositet
Cremethed
Eftermundfylde
Meta descriptor
Total fattiness
Samlet fedhed
In Danish, no word for avour exists. Flavour is expressed through the Danish term for taste, smag.
330
correlation loading plots from four signicant dimensions (explaining in average 60, 15, 4.2 and 4.1% of the
variation in sensory data, respectively). The observed
eects become much more apparent when looking at
score plots for the four dimensions, when the dierent
factors are labelled separately and products are grouped
by factors or combinations thereof. These are shown in
Figs. 1b,c and 2b,c, and are referred to along with the
explanations in the text. All other combinations of
dimensions were explored during data analysis, but the
combinations shown, 12 and 34, proved sucient for
interpretation of the data.
In dimension 1, two major clusters of descriptors are
seen. One group consisting of Boiled milk smell and
avour, Transparency and Blueness relates to 0.1% fat
milk. At the opposite end of the rst dimension is a
group of descriptors all relating to 3.5% fat milk, consisting of Sweet taste, Creamy smell and avour,
Thickness visual and oral, Glass coating, Creaminess,
Residual mouth ll, Total fattiness and Yellowness.
This indicates a clear fat-level-direction in the results
(Fig. 1b). The distances between groups with dierent
fat levels show that there is a much larger dierence in
fattiness between 0.1 fat and 1.3% fat than there is
between 1.3 and 3.5%. This indicates that perceived
fattiness is not a linear function of actual fat content in
the range spanned by the milk in our experiment,
which is the range of milk fat content normally sold in
Denmark.
The combined eects of thickener, whitener and
homogenisation result in a direction orthogonal to that
of fat level (Fig. 1c). This direction is mainly a dierence
in perceived whiteness. Samples with the addition of
those three factors mainly score higher in whiteness,
whereas those without, score lower. In Fig. 1c the factors are grouped, so the eect of the individual factors
can be seen. From this, it is evident that whitener and
thickener contribute to fattiness. There seems to be a
small eect of homogenisation as well. Signicant differences in a few sensory descriptors were observed.
Homogenisation gives an increase in Creamy avour
and a decrease in Blueness, both of these dierences
were only observed in 1.3% fat milk (Table 3). The
eect of whitener and thickener is an increased perceived whiteness of the milk, as well as some increase in
the group of high fat related descriptors. In this direction, no separation of thickener and whitener is seen,
this subject will be returned to in dimension 4 below.
The third dimension spans the variation between
samples with and without added aroma compounds, so
this is a avour dimension (Fig. 2b). Samples with
added aroma compounds have a higher intensity of
Creamy smell and avour. Notice also that sweet taste
correlates with the creamy descriptors (Fig. 2a). However, the absolute dierences in sweetness are smaller
than the dierences in creamy smell and avour (cf.
Table 3
Mean values (over panellists and replicates), Least Signicant Dierences (LSD) values (P<0.05) for all products and descriptors, product specications and product codesa
Product names
Product description
Sensory descriptors
Aroma
Thickener
Whitener
Aroma
Creamy smell
0.1
1.3
3.5
0twAh
0tWaH
0TwaH
0tWAH
0TWAH
1twaH
1twAh
1tWAH
1TwAH
1TWaH
3twaH
3TwaH
3TWAH
0.1
0.1
0.1
0.1
0.1
1.3
1.3
1.3
1.3
1.3
3.5
3.5
3.5
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
Product description
Whiteness
Yellowness
1.12
3.56
5.24
6.11
0.97
6.20
5.79
4.43
0.78
6.02
6.39
6.27
0.85
4.27
5.84
8.05
0.76
6.45
4.18
2.69
0.74
10.40
7.99
5.24
0.95
2.53
6.55
9.65
0.90
3.31
6.56
8.79
5.58
4.19
3.76
6.53
6.42
6.16
7.89
8.44
8.36
6.36
7.21
7.04
9.07
5.88
6.05
6.38
5.34
5.50
4.86
4.42
3.86
4.35
3.91
4.14
4.47
3.59
6.02
8.26
5.75
7.64
7.73
6.36
6.34
7.43
6.71
8.13
6.38
6.89
7.63
4.79
4.16
4.18
5.12
4.89
6.17
6.56
6.41
6.68
5.72
7.67
7.47
6.64
6.04
4.09
6.96
3.86
4.14
3.37
3.65
2.96
3.09
2.68
3.04
2.54
2.47
10.08
7.93
10.48
8.14
7.95
7.36
7.91
6.44
7.21
5.81
5.85
5.07
3.99
3.06
4.08
2.41
4.76
5.04
6.77
7.10
8.41
7.83
8.62
9.46
10.46
10.41
3.90
5.47
3.65
5.41
6.49
7.14
6.67
8.19
7.45
8.31
8.45
9.51
9.84
Thickener
Whitener
Aroma
Homogenisation
LSD (5%)
0twah
1twah
3twah
0.1
1.3
3.5
0twAh
0tWaH
0TwaH
0tWAH
0TWAH
1twaH
1twAh
1tWAH
1TwAH
1TwaH
3twaH
3TwaH
3TWAH
0.1
0.1
0.1
0.1
0.1
1.3
1.3
1.3
1.3
1.3
3.5
3.5
3.5
+
+
+
+
Transparency
Glass
coating
Thickness
visual
Residual mouth ll
Total fattiness
Sensory descriptors
Flavour/Taste
Blueness
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
Creamy avour
Texture/Mouthfeel
Boiled milk avour
Sweet taste
Thickness oral
Creaminess oral
1.08
1.01
0.72
0.88
0.99
1.00
1.03
3.46
5.21
8.05
7.06
6.08
5.07
5.04
5.91
6.47
3.23
6.43
8.81
3.14
6.14
8.85
3.34
5.99
8.24
3.26
5.94
8.97
5.73
4.58
3.36
6.12
6.61
6.86
8.14
8.60
8.86
7.56
8.20
8.93
10.05
6.58
6.49
7.05
6.09
6.25
5.34
4.67
4.80
4.58
4.74
4.53
4.19
3.57
6.85
5.53
4.97
6.38
6.31
6.41
7.39
7.14
7.46
6.39
6.89
7.22
8.07
4.09
5.04
3.41
5.04
5.90
6.57
6.56
8.08
7.87
8.27
8.59
9.62
9.56
4.89
4.50
3.18
4.90
6.51
6.77
7.30
8.47
8.81
8.11
8.66
9.76
10.39
4.88
4.13
3.05
5.00
5.97
6.09
6.95
8.26
8.01
7.67
8.29
9.11
9.69
4.73
4.44
2.89
5.24
6.76
6.87
7.49
8.59
8.96
7.94
8.76
9.64
10.69
331
a
Product abbreviations refer to the levels of the factors (0.1 and 3, 0.1, 1.3 and 3.5% fat, respectively; t and T, none and addition of thickener; w and W, none and addition of whitener; a and A, none and addition of aroma
compounds; h and H, no homogenisation and homogenisation).
LSD (5%)
0twah
1twah
3twah
Appearance
Homogenisation
332
Fig. 1. (a) APLSR correlation loadings for the two rst dimensions showing dierences among the 16 products. ~ Sensory Descriptors, & Factors
and * Products. For clarity of this gure product names are not shown. The inner and outer circles represent 50 and 100% explained variance,
respectively. (b) Score plot from APLSR. Dimension I and 2. Indicating dierences in fat levels (0=0.1%, 1=1.3% and 3=3.5%), and showing fat
level direction based on the loadings. (c) Score plot from APLSR. Dimension I and 2. Indicating dierences in addition of thickener (t/T), whitener
(w/W) and homogenisation (h/H). Capital letters indicate addition of the factor. Showing whitener and thickener level direction and homogenisation
level direction based on the loadings.
333
Fig. 2. (a) APLSR correlation loadings for components 3 and 4 showing dierences among the 16 products. ~ Sensory Descriptors, & Factors and
* Products. For clarity of this gure product names are not shown, and only relevant descriptor names are shown. The inner and outer circles
represent 50% and 100% explained variance, respectively. (b) Score plot from APLSR. Dimension 3 and 4. Indicating addition of aroma compounds (a/A). Capital letters indicate addition of the factor, and showing aroma level direction based on the loadings. (c) Score plot from APLSR.
Dimension 3 and 4. Indicating dierences in addition of thickener (t/T) and whitener (w/W). Capital letters indicate addition of the factor.
334
the same dierences that were apparent from the correlation loading plots (Fig. 1a) and the score plot with
labels for fat content (Fig. 1b). A rather large group of
descriptors is positively correlated with fattiness (Residual mouth ll, Creaminess, Thickness visual/oral,
Creamy smell and avour, Glass coating and Yellowness). Another group of descriptors is inversely correlated with a high fat content (Boiled milk smell and
avour, Blueness and Transparency). All of these
descriptors vary signicantly (P<0.05) across the three
products. Whiteness is not aected signicantly by the
changes in fat content. It is also, as shown in APLSR,
evident that the sensory dierences between 0.1 and
1.3% fat are larger than between 1.3 and 3.5% fat. This
is conrmed by the signicant dierences between products observed by ANOVA. Fig. 4 shows the sensory
prole of 0.1% fat with all added additives and 1.3%
fat, homogenised. There are only signicant dierences
in two descriptors. The 0.1% fat milk with additions
have a higher Whiteness, but a lower Glass coating. The
Total fattiness is the same, so the sensory properties of
1.3% fat have been mimicked quite successfully by the
addition of all the food additives used in the experiment.
This was not the case with only one food additive added
(Fig. 5). Addition of whitener and aroma were not sufcient either to mimic the increase in true fat content
from 0.1 to 1.3%, since there is a signicant dierence
Fig. 3. Cobweb plot of sensory proles from the three dierent fat levels.
Fig. 4. Cobweb plot of sensory proles from 1.3% fat, homogenised and 0.1% fat added all additives.
Fig. 5. Cobweb plot of sensory proles from 1.3% fat, homogenised and 0.1% fat added only one of the food ingredients.
335
336
Acknowledgements
This work is part of the FTEK programme supported by the Danish Dairy Research Foundation
(Danish Dairy Board) and the Danish Government.
Nina Ahn, Judith Henning and Anne Marie Laustsen
are thanked for technical assistance. Ditte Marie Folkenberg is acknowledged for inspiring the rst author to
using the meta-descriptor Total fattiness. The donation of food additives from Danisco Cultor is highly
appreciated.
References
4. Conclusions
The results from the experiment show that the sensory
properties of fat in milk are comprised of texture/
mouthfeel, appearance and avour attributes. Analysis
of the contribution of the individual sensory descriptors
to perceived fattiness in milk shows that Creaminess
and Residual mouth ll most accurately reect fattiness
of milk in this fat range. It is unknown to what extent
the results about perceived fattiness from a sensory
panel can be extended to the general population. However, the assessment of fattiness obtained from a trained
sensory panel gives a very detailed picture of human
sensory perception of fattiness. The sensory dierences
between 0.1% fat milk with added thickener, whitener
and aroma are very small as compared to those in
homogenised 1.3% fat milk. They only dier signicantly in two descriptors: Whiteness and Glass coating. It was not the case when only using one food
additive, or combinations of two. This clearly shows
that the sensory properties of fat in milk are not easily
substituted with food additives seeking to imitate the
sensory properties of fat. The experiment showed that
there are larger sensory dierences between milk with
0.1% and 1.3% fat, than there are between 1.3% and
3.5% fat. This shows that the fat does not aect the
sensory properties of milk in a linear fashion. It is very
likely that sensory properties of 0.1% fat milk can be
changed drastically by adding only a little extra fat to
the milk.