Assignment

Clinical Pharmacy (Th)

Unorganized Abstract:
Melatonin protects against ischemia/reperfusion injury in skeletal muscle

Melatonin has been shown to diminish ischemia-reperfusion (I/R) injury in many tissues. The
main aim of this study was to evaluate the protective antioxidant effect of melatonin in skeletal
muscle during I/R injury. Wistar albino rats were randomly divided into three groups. Hindlimb
ischemia was achieved by clamping the common femoral artery in two groups but not in control
group. Limbs were rendered ischemic for 1.5 hr; at the end of the reperfusion period of 1.5 hr
muscle tissue samples were taken for the histological evaluation and biochemical analysis.
Melatonin (10 mg/kg) was injected i.p. in the I/R + Mel group at the onset of ischemia whereas
the vehicle solution was injected in the I/R group. In I/R + Mel group histological damage was
significantly less than in the I/R group (P < 0.001). In the I/R + Mel group, the mean
malonedialdehyde level was lower than in the I/R group (P < 0.01) and was quite near to the
levels in the control group (P > 0.05). Glutathione levels were found to be reduced in the I/R
group compared with the control (P < 0.01) and I/R + Mel group (P < 0.01). Melatonin has a
protective effect against I/R injury in skeletal muscle and may reduce the incidence of
compartment syndrome, especially after acute or chronic peripheral arterial occlusions.

Organized Abstract:
Melatonin protects against ischemia/reperfusion injury in skeletal muscle

Korhan Erkanl1, Nihan Kayalar1, Gzde Erkanl2, Feriha Ercan2, Gksel Sener3and Kaan
Krali1
Introduction:
Melatonin has been shown to diminish ischemia-reperfusion (I/R) injury in many tissues. The
main aim of this study was to evaluate the protective antioxidant effect of melatonin in skeletal
muscle during I/R injury.
Methodology:
Wistar albino rats were randomly divided into three groups. Hindlimb ischemia was achieved by
clamping the common femoral artery in two groups but not in control group. Limbs were
rendered ischemic for 1.5 hr; at the end of the reperfusion period of 1.5 hr muscle tissue samples
were taken for the histological evaluation and biochemical analysis. Melatonin (10 mg/kg) was
injected i.p. in the I/R + Mel group at the onset of ischemia whereas the vehicle solution was
injected in the I/R group.
Result:
In I/R + Mel group histological damage was significantly less than in the I/R group (P < 0.001).
In the I/R + Mel group, the mean malonedialdehyde level was lower than in the I/R group
(P < 0.01) and was quite near to the levels in the control group (P > 0.05). Glutathione levels

BY: SYEDA BASMA JAVED (1112828)

Assignment

Clinical Pharmacy (Th)

were found to be reduced in the I/R group compared with the control (P < 0.01) and I/R + Mel
group (P < 0.01).
Discussion:
Melatonin has a protective effect against I/R injury in skeletal muscle and may reduce the
incidence of compartment syndrome, especially after acute or chronic peripheral arterial
occlusions.
Keywords: ischemia, lipid peroxidation;, melatonin, reperfusion injury, skeletal muscle

BY: SYEDA BASMA JAVED (1112828)