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doi:10.1088/0957-0233/24/2/025301
1. Introduction
0957-0233/13/025301+10$33.00
D Liang et al
(2)
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(3)
Figure 1. Plot of a typical measurement: balance readouts (dots)
before and after dispensing are displayed as a function of time.
Readouts acquired between the two vertical lines are not used for
calculation (settling time of the balance).
n
b=
1
i=1 i2
n
n
1
i=1 i2
ti Ii
i=1 i2
n
ti2
i=1 i2
n
ti
i=1 i2
n
n
ti
i=1 i2
Ii
i=1 i2
2
(5)
(4)
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(7)
Dprog (t ) = tn2,1 2 s
Here, tdisp indicates the moment when the dispense of the liquid
volume is triggered, which isexcept for an offset of a few
millisecondsthe point in time when the liquid just impinges
on the weighing dish.
Denoting the values estimated by the linear regression by
subscripts LR, m is supposed to be the difference of these
two linear regression values at time tdisp :
m = mLR,after (tdisp ) mLR,before (tdisp ).
1+
(t t )2
1
+ n
,
2
n
i=1 (ti t )
(9)
where
n
1
(mi mLR,i )2 ,
s=
n 2 i=1
(8)
1
ti .
n i=1
n
t=
(10)
D Liang et al
Table 1. Uncertainty calculation of GRM for a typical measurement (the same measurement data as shown in figure 1).
Input quantity ( )
Unit
Value
Standard uncertainty u( )
m
m
w
a
c
g
g
g nl1
g nl1
g nl1
1
C
199.0
0
0.996 6465
0.0012
8.006
0.000 207
26.7
5.9842
3.05
1.52E-06
2.89E-07
0.01
2.89E-07
0.05
V20
u(V20)
U(V20), k=2
nl
nl
nl
199.6
6.7
13.5
V20 2 2
V20 2 2
u(V20 ) =
u (m) +
u (m)
m
m
2
2
V20
V20
u2 (w ) +
u2 (a )
+
w
a
V20 2 2
V20 2 2
u (c ) +
u ( )
+
c
V20 2 2
u (T ),
+
T
where
Sensitivity coefficient
1.0030
1.0030
200.5053
172.3952
0.0037
1339.1259
0.0414
even larger than u(m) for the presented case, it is evident how
important it is to consider the error from the regression analysis
and the prognostic calculation correctly, in order to avoid
underestimation of the uncertainty of the final measurement
result.
To enable the direct comparison with the Artel MVSfor
which the uncertainty is provided by the manufacturer for the
95% confidence interval (i.e. expanded uncertainty, k = 2)
the uncertainty for the GRM will also be discussed in terms
of the expanded uncertainty which can be calculated from
the standard uncertainty u(V20 ) by multiplication with k = 2:
U (V20 ) = 2 u(V20 ).
(11)
4. Experimental section
The primary measurement device of the gravimetric
liquid measurement system used in this study is a
high precision laboratory micro balance type XP2U/M
from Mettler Toledo, Switzerland. A micro weighing dish
(ID H 13 mm 3.5 mm, VWR Order no 6111356)
filled with about 200 l of the same testing liquid that is
provided to the dispensing device (i.e. deionized water or
Artels proprietary dyewater mixture) is placed at the center
of the weighing pan and is covered by an aluminum draft
shield (ID H 80 mm 24 mm) with a circular opening
( 6 mm) on the top. The testing liquids involved in this
experiment are deionized water with water quality of 18 M
D Liang et al
Figure 2. Experimental setup for gravimetric measurement as well as for micro plate preparation for subsequent photometric evaluation
(equipment of Artel-MVS not shown). Di-H2O: laboratory deionized water.
D Liang et al
Figure 4. Bias and CV of the presented gravimetric measurement method compared to the Artel-MVS method. Environmental conditions
during the measurements: temperature = 29.4 C, relative humidity = 51%, air pressure = 975 hPa. GRM: gravimetric regression method.
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6. Conclusion
In this paper, we have presented an improved gravimetric
volume measurement method referred to as gravimetric
regression method (GRM) for determining liquid volumes in
the sub-l range. The core element of this method is a linear
regression for evaporation compensation applied on a large
number of measured data points before and after addition
of the liquid volume to the balance. In order to predict the
Figure 6. Bias and CV of the presented gravimetric measurement method carried out with Di-H2O and Artel sample solution.
Environmental conditions for measurement with Artel sample solution: temperature = 29.4 C, relative humidity = 51%, air pressure =
975 hPa. Environmental conditions for measurement with Di-H2O: temperature = 26.7 C, relative humidity = 52%, air pressure =
975 hPa. GRM: gravimetric regression method. Di-H2O: laboratory deionized water.
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Acknowledgment
The authors gratefully acknowledge financial support from the
German Federal Ministry for Science and Education (BMBF)
through the project Smart Reagent Dosage (SFK 16SV5119).
Also, we would like to thank our project partners from
Roche Diagnostics GmbH, Mannheim, Germany, for their kind
support and valuable feedback.
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