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Introduction
Significant advances in the development of new
strains and vectors, improved techniques, and the
commercial availability of these tools coupled with a
better understanding of the biology of Pichia species
have led the value and power for the microbe in
commercial and research laboratories1. In the Biotech
Century, scientists are continuing to engineer yeasts
for producing eukaryotic foreign proteins. Yeast
offers the ease of microbial growth and gene
manipulation found in bacteria along with the
eukaryotic characteristics2. Yeast combines molecular
genetic manipulations and growth characteristics of
prokaryotic organisms together with the sub-cellular
machinery for performing post-translational protein
modifications of eukaryotes. Because of the vast
knowledge in genetics and physiology of
Saccharomyces cerevisiae, it was selected first for
______________
*Author for correspondence:
Tel: 91-80-23418078; Fax: 91-80-23412509
E-mail: veluvarthy@yahoo.com
Present Address:
1
Division of Virology Indian, Veterinary Research Institute,
Mukteswar, Nainital 263 138, India
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P. methanolica
P. stipitis
Promoter Regulation
References
AOX 1
FLD 1
GAP
PEX 8
YPT1
Methanol induced
Methanol or methylamine
induced
Strong constitutive
Moderate methanol
induced
Moderate constitutive
AUG 1 Methanol induced
XYL1 Xylose induced
7
36
35
39
40
31
85
Vaccines /Antigens
0.4
Hepatitis B surface antigen
Pertussis Ag (Pertactin) P69
3.0
Tetanus toxin fragment C
12.0
HIV-1 gp120
1.25
Tick anticoagulant protein
1.7
Bm86 tick gut glycoprotein
1.5
Bovine herpes virus-1
0.01
0.1
Dengue viral envelope
proteins
FMD viral proteins
0.15-2
Pseudorabies virus gE
10
P. falciparum antigens
0.5-2.6
*I Intracellular, S secreted
I
I
I
I
S
S
S
S
64
53
9
22
50
49
54
60,62
S
S
I/S
81,82
86
72,77
179
180
181
182
183
184
31
32
33
34
35
36
37
38
39
40
41
42
43
44
45
46
47
185
186
64
65
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85
86