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Breeding and Producing

Elite Seedlings
for Jatropha Plantation

Dr. HONG Yan


General manager
(Research and Operation)
Email: hongy@joil.com.sg
www.joil.com.sg
JOil presentation for JATROPHAWORLD 2010, Rotterdam

Introduction to Jatropha curcas


A small tree or large shrub originated from central America
Widely introduced into tropical and subtropical countries
It adapts well in marginal and degraded soils, can survive arid
and semiarid conditions
Traditional uses:

Living fence and hedge plants


Reclaiming eroded land
Folk medicine
Soap making, lighting and cooking fire
Cake as a good fertilizer

Holds great potential as a biodiesel feedstock plant

JOil presentation for JATROPHAWORLD 2010, Rotterdam

Jatropha curcas, a good choice of


biodiesel feedstock plant
Feedstock Attributes
Attribute
Fit for EU Sustainability
Cost
Availability
GHG Footprint
Perennial
Non-Food
Freezing Point
Jet Fuel

Rapeseed
X X X
X X X
9 9 9
X X X
X
X
o
- 10 C
X

Palm
X X
X X
9 9
X X
9
X
o
10C
X

Soya
X
X
9
X
X
X
o
-2 C
X

Jatropha
9 9 9
9 9 9
X
9 9 9
9
9
o

- 2 C*
X

JOil presentation for JATROPHAWORLD 2010, Rotterdam

JOil (S) Pte. Ltd.


A joint venture private limited company incorporated by TLL, TATA
chemicals, Toyota Tsusho and other partners
Headquartered in Singapore, the company will have its business in
various countries and regions suitable for jatropha plantation
Members of Roundtable for Sustainable Biofuel (RSB) and Jatropha
Alliance
Core activities :
R&D to improve Jatropha through traditional breeding and genetic
modification
Jatropha tissue culture technology
Production and sale of elite Jatropha seedlings
Consulting services for Jatropha plantations
Has the exclusive commercial rights for jatropha R&D in TLL

JOil presentation for JATROPHAWORLD 2010, Rotterdam

Positives

Jatropha
today

Negatives
Uncertai
economics n
d
low produ ue to
ctivity

Nonedible
(food vs. Fu
el)
Can grow on
marginal la
nd
(food vs. Fu s
el)

Harvesting
Storage issu &
es

Rural
developmen
t

Toxicity issu
es

JATROPHA

JOil presentation for JATROPHAWORLD 2010, Rotterdam

Jatropha Value Chain


Planting
materials

7%

Land
management

33%

7%

32%

Oil Extraction

Harvesting

Plantation &
crop
maintenance

4%

JOil presentation for JATROPHAWORLD 2010, Rotterdam

Planting
material
plays a key
role in
success of
entire
value chain

Biodiesel
Manufacturing

19%
Basis : Cost Allocation based on test plantations

What Biotechnology can do to Jatropha..


Developing good hybrids
Accessions from all over the world, comparison of performance and selection
of best performing accessions
Controlled pollinations
Markerassisted breeding using genetic markers

Tissue culture propagation of elite hybrids


Addresses uniformity
Fast multiplication for large scale production
Shortens breeding cycle

Genetic modification (GM)


Pathogen tolerance, higher seed yield, lower toxicity, higher oil
content

JOil focuses on all the three aspects


JOil presentation for JATROPHAWORLD 2010, Rotterdam

Strategy I: Combining the best traits


First generation Variety I:
varieties:

#High number of flowers but


medium level of female flowers
#Soft branch

Second generation
hybrids:
#high number of flowers +
high female flower ratio
#stronger branches

JOil presentation for JATROPHAWORLD 2010, Rotterdam

Variety II:

#Higher female flower ratio


#vigorous growth with strong branch

Strategy II: Introgression breeding through


interspecies hybridization

1.1 m

One BC2F1 plant (~9 mth old)


-Compact size with strong branches
-Very high female flower ratio,
-Flowering bunches are close
-Clearly identifiable traits
JOil presentation for JATROPHAWORLD 2010, Rotterdam

Strategy III: Improving local varieties

Cross pollination
between our best
material with local
varieties collected from
our markets
To create hybrids of
higher productivity, also
with local adaptation
Hybrid vigor found
between our best hybrid
(SEA origin) and Indian,
Chinese and African
collections

JOil presentation for JATROPHAWORLD 2010, Rotterdam

A hybrid with Indian heritage

Strategy III: Improving local varieties

A hybrid with African heritage

A hybrid with Chinese heritage


JOil presentation for JATROPHAWORLD 2010, Rotterdam

How to provide plantation materials?


Propagation
methods

Advantages

Disadvantages

-Easy to carry around


and storage
-Cost effective

-Variable performance in field due to


segregation
-Potential inbreeding depression with
pure line approach
-Long breeding cycle for hybrid
approach
-loss of viability

Stem cutting

-Cost effective
-Easier to practice

-Slow and less efficient propagation


-Weaker rooting

Tissue culture
(our choice)

-High propagation ratio


-True-to-type cloning
-Strong rooting
-Short breeding cycle

-Technical demanding
-Higher cost

Seeds

JOil presentation for JATROPHAWORLD 2010, Rotterdam

JOil proprietary

Jatropha curcas
in vitro propagation

JOil presentation for JATROPHAWORLD 2010, Rotterdam

Jatropha clones 4
month after
transplanting

JOil presentation for JATROPHAWORLD 2010, Rotterdam

Jatropha tissue culture


technology of JOil
A leader in commercial scale production
To avoid natural segregation in seed derived plants
Fast, with high propagation ratio and wide
applicability to various jatropha varieties
Produces hundreds of thousands of truetotype
clones from a single elite hybrid plant within a year

Features of the phase 1 varieties


Natural branching
Early flowering and
Good fruiting
JOil presentation for JATROPHAWORLD 2010, Rotterdam

TLL/JOil jatropha
Experiment
Station in
Singapore

2009

2010
JOil presentation for JATROPHAWORLD 2010, Rotterdam

Global (Asia and Africa) Operations


India

Lab Pune
Trials - Aurangabad

China

Lab Shanghai

Thailand

Lab Bangkok

Phillipines

Lab Davao

Kenya

Lab Nairobi
Trial Kajiado

JOil presentation for JATROPHAWORLD 2010, Rotterdam

JOil : Key Considerations

Superior technology
Reputed shareholders
Committed management team
Global operations
Key success factors
Leveraging on strong partners
Performance backed products (through field trials, demo
projects)
Continuous Research & Development

JOil presentation for JATROPHAWORLD 2010, Rotterdam

How we can work together


to make Jatropha viable
Introduction of new varieties, collaboration in field trials
Technical services:

Due diligence services for existing Jatropha plantations


Technical consulting for undertaking new Jatropha plantations
Technical consulting for improving existing Jatropha plantations
Seed quality traits analysis

Licensing in, licensing out for new variety and technology


OEM production and contract research
Tissue culture facilities (purchase / lease / JV)
Many more

JOil presentation for JATROPHAWORLD 2010, Rotterdam

Thank You!

JOil presentation for JATROPHAWORLD 2010, Rotterdam