What is A Phagemid Vector

This article introduces some basic information of phagemid, and phagemid vector, as well as the related
biotech company--Creative Biolabs.
1. About Phagemid
Before introducing the phagemid, it is important for us to know something related to it--the Plasmid. As for
the Plasmid, it is a small circle of bacterial DNA that is independent of the main bacterial chromosome.
Plasmids often contain genes for drug resistances and can be transmitted between bacteria of the same
and different species and have been frequently used in genetic engineering.
Following is the Phagemid. A phagemid is a plasmid that contains an f1 origin of replication from a f1
phage. It can be used as a type of cloning vector in combination with filamentous phage M13. A phagemid
can be replicated as a plasmid, and also be packaged as single stranded DNA in viral particles. Phagemids
contain an origin of replication (ori) for double stranded replication, as well as an f1 ori to enable single
stranded replication and packaging into phage particles. Many commonly used plasmids contain an f1 ori
and are thus phagemids.
In a simple way to understand about is that a phagemid is a normal plasmid which additionally has the
feature of an M13 origin. The M13 phage origin allows the packaging of the plasmid into an M13 phage
when the bacteria is also infected by an M13 helper phage. Phagemids usually encode no or only one kind
of coat proteins. Other structural and functional proteins necessary to accomplish the life cycle of phagemid
are provided by the helper phage.
2.Phagemid vectors
Phagemid vectors are plasmids which have been artificially manipulated so as to contain a small segment
of the genome of a filamentous phage, such as M13, fd or f1. Those phagemid vectors that are commonly
used include the pEMBL series of plasmids and the pBluescript family .
Usually, phagemids are used to generate templates for site-directed mutagenesis. It can also be applied
into RNA transcription, high-resolution restriction mapping, sequencing of single- and double-stranded
nucleic acids, generation of nested deletions,etc.
To find a leading biotech company providing you high quality Phagemid vector, Creative Biolabs, supplying
quality phagemid vectors for phage display and related applications, is your good choice. It has the Original
pCombs, pComb3X Set, pComb3H Set, and some Other choices. We are also professional in conducting
in depth antibody humanization and affinity maturation using phage display and DNA mutagenesis
approaches. In addition, OEM services for bulk scale antibody manufacturing are also available at the most
competitive price in the industry.
For More Information regarding Phagemid vectors Kindly visit our website:- http://www.creativebiolabs.com/