Aloe Vera

HERBAL MONOGRAPH OF ALOE BARBADENSIS
(syn. ALOE INDICA)

Dried juice of leaves of Aloe barbadensis Mill.(Syn. A.vera(Linn).Burm.f.,
A.indica Royle); Fam.Liliaceae. The plant is distributed throughout India.
INDIAN NAMES
Sans.
Beng.
Guj.
Hindi
Kan.
Mal.
Mar.
Ori
Tam.
Tel.
:
:
:
:
:
:
:
:
:
:
Kumarirasasambhava, Ghrita- Kumari

Ghrita kumari
Kunwar
Musabhar, Ghikanwar
Kolasoare, Komarika, Maulisara
Kattavazha
Korphad
Kumari, Mushabora
Chirukattalai, Kattalai
Kalabanda
CHEMICAL CONSTITUENTS
Major constituents includes Glycosides- Anthracene derivatives:
Hydroxyanthraquinone derivatives(25-40) viz., aloin (=barbaloin, a mixture of
aloin A & B, the diastereoisomeric 10-C glucosides of aloe-emodin anthrone)
and 7-hydroxyaloin isomers.Indian aoles contain aloinosides as major
constituents with only traces of aloin.
Minor constituents includes aloeemodin, chrysophanol; chromone derivatives
viz., aloeresin B (=aloesin, upto 30%) with its p-coumaryl derivatives aloeresins
A & C and the aglycone aloesone.
IDENTITY TESTS (FROM RAW MATERIALS)

The drug answers tests for anthraquinone glycosides.
Mix 0.5g with 50ml water, boil until nearly dissolved, cool and add 0.5gm of
Kieselguhr and filter.
a) Heat 5ml of the filtrate with 0.2g of borax until dissolved, add a few
drops of this solution to a test tube nearly filled with water, a green
fluorescence is produced.
b) Mix 2ml of the filtrate with 2ml freshly prepared solution of bromine; a
pale yellow precipitate is produced.
ASSAY / ANALYTICAL METHODS (FROM EXTRACTS)
1) Ascorbic acid estimation by HPLC method
HPLC System
: Shimatzu 2010 CHT
HPLC with UV detector at 254 nm
Column
: C-18
Mobile phase
: 5% Methanol in 0.01 M KH2PO4
Flow rate
: 1ml/min
Retention time
: 2.60min
Standard
: Ascorbic acid
As it is difficult to obtain an accurate quantification from the extract using
Standard method, most companies do not carry out any analysis for the key
phytochemical marker. Konark Herbals and Health Care believes in providing
2
highly standardized extracts and hence all the quantification are done using
HPLC.
HPLC GRAPH -
2) Aloin estimations by Gravimetric method.

3) Total phenolics by spectrophometric method
KHHC constantly innovate and upgrades its products and testing methods. We
suggest you to review our website providing regular updates.
PESTICIDES LIMITS
A limit for pesticide is one of the major issues in standardization of medicinal
plants and products in view of the worldwide widespread use of pesticides in
cultivated plants. The presence of pesticides in extracts increase the health risk
by many folds. The pesticides can be extremely irritant on skin as well as in the
internal organs, it is essential to monitor its concentration as a part of GMP.
Various analytical methods for the quantitative determination of pesticides by
gas chromatography coupled with mass-spectrophotometer are in use. Konark
Research Foundation (KRF), a NABL certified lab is well equipped with the
latest technology and instruments and monitors the pesticide limit as part of its
GMP.
CHROMATOGRAPHIC PROFILE
From the pharmacopoeial perspective, a better quality control of raw material
can be achieved by specifying a quantitative test procedure for the
determination of the range or a minimum content by specifying a quantitative
test procedure for the determination of the range or a minimum content of the
marker substances or the active ingredient. A chromatographic finger profile
represents qualitative/ quantitative determination of various components present
in a complex plant extract irrespective whether or not their exact identity is
known. Thin layer chromatographic technique is the simplest and least
expensive method that provides plenty of information on the composition of
raw herbs and its preparation. For quantitative analysis of active ingredients or
marker substances with simultaneous separation and detection High Pressure
liquid chromatography is the best technique. We use the latest model of HPLC
for all its analysis.
LIMITS FOR IMPURITIES
A test requirement for foreign organic matter would ensure the extent of
contamination of extraneous matters such as filth and other parts of botanicals
not covered by the definition of the herbal drug. Since sand and soil are
predictable contaminants of botanicals, test requirements for total ash, water
soluble ash, acid soluble ash, residue on ignition and sulphated ash would be
expected to limit such contaminants.Test requirement for heavy metals in
botanical raw material are probably more relevant for parts of plants growing
under ground than for the aerial parts of the plant. The presence of high levels
of minerals interact with the final product there by affecting its keeping quality.
MICROBIAL LIMITS
If the raw herb is to be used directly without boiling in water prior to
consumption, restrictive limits on microbial contaminants are required for
pathogens such as Salmonella sp. Enterobacter and E.coli which are causative
agent for various gastro-intestinal diseases. A lower level of yeasts and molds
and a limit on total aerobes are considered appropriate in plant material for
topical use. The presence of aflatoxins detected by chemical means is generally
4
independent of the number of viable molds that are detected using

microbiological methods. Aflatoxins in microgram quantity are capable of
giving serious hypersentivity reactions which can be extremely harmful to
human health.
APPLICATION
Cathartic action of the drug, limited to large intestine, is attributed to
anthraquinone glycosides, chiefly aloin. Latter is not absorbed in the upper gut
but hydrolysed to the active aglycone at the site of action in the colon and
rectum by intestinal bacteria.The anthrones irritate the mucus membrane,
leading to an increase in the secretion of mucus, thus stimulating peristalsis.
Also they induce an active secretion of water and electrolytes into the lumen of
the gut and inhibit the absorption of electrolytes and water by the colon.
REPORTED ACTIVITIES
Immunomodulatory,
Wound healing,
Anticancer,
Radioprotective efficacy,
Angiogenic,
Antiviral,
Analgesic
Anti-inflammatory,
Hypotensive.
USES
Burn and Wound Healing: Typically the wound healing requires
regeneration of skin and closing of the wound. Aloe Vera is soothing in
nature due to its moisture content. Studies show that Aloe Vera when
applied to a wound increases the rate of wound closure and enhances the
proliferation of cells, whereby the healing is enhanced.
Aging of the Skin: Components of Aloe Vera have been found to reverse
degenerative skin changes by stimulating collagen and elastin synthesis.
Immune System Restoration: Research has proven that Aloe Vera
improves skin's immune system.. It protects against the UV radiation.
Moisturizer: Aloe Vera improves the skin's ability to hydrate itself, aids
in the removal of dead skin cells and has an effective penetrating ability
that helps transport healthy substances through the skin.
Arthritis, Joint and Muscle Pain: Aloe Vera is believed to reduce
severe joint and muscle pain associated with arthritis, tendinitis and other
injuries. When applied directly to the area of pain, Aloe Vera penetrates
the skin to soothe the pain.
Relieve Gastrointestinal Problems:
Coronary Heart Disease Studies suggest that the ingestion of Aloe Vera
gel may have a beneficial effect to the accumulation of blood fat lipids
associated with the disease.
Blood circulation - a number of constituents have beneficial effects on
blood pressure and coagulation.
Digestion - One of its most popular uses is in helping any type of
digestive or bowel disorder. It has a gentle laxative effect on the bowels.
For Konark complexes like- Konark DAN.
IMPORTANT SITES FOR RESEARCH IN ALOEVERA PRODUCTS
1)http://www3.interscience.wiley.com/journal/117953984/abstract?CRETRY=1
&SRETRY=0
Anti-sickling potential of Aloe vera extract
The effect of Aloe vera extract on the gelling time of human HbSS erythrocytes
was investigated. The results showed that A.. vera extract increased the gelling
time of HbSS blood and inhibited sickling in vitro. In addition, a linear
relationship was found between extract concentration and gelling time,
suggesting that A.. vera extract may have great potential in the management of
sickle cell disease.
2) http://www.internethealthlibrary.com/Plant-Remedies/AloeVeraresearch.htm
Some recent studies appear to show topical and orally administered aloe vera
preparations in patients with angina and heart disease, diabetes, psroiasis,
wound healing and chronic venous leg ulcers.
3) http://www.dietahoodia.com/dieta-hoodia/investigaciones---research-paperson-hoodia-aloe-chia-noni/investigaciones---research-papers-on-aloe
a) J Wound Care. 2004 Apr;13(4):157-8.: Phytotherapy: an alternative

treatment for non-healing ulcers.Avijgan M.Shahr-e-kord University of Medical
Sciences, Shahr-e-kord, Iran. avijgan@yahoo.com
b) Int Immunopharmacol. 2004 Mar;4(3):411-8.: Mannan from Aloe saponaria
inhibits tumoral cell activation and proliferation.
c) Effects of Aloe Extracts on Human Normal & Tumor Cells In-VitroWinters
WD; Benavides R; Clouse WJ (Dep. Microbiol., Univ. Tex. Health Sci. Center
Econ Bot 35 (1), 1981, 89-95).
d) Anticancer Effects of Aloe on Sarcoma 180 in ICR Mouse & On Human
Cancer Cell Lines. (Jeong HY; Kim JH; Hwang SJ; Rhee DKColl. Pharm.,
Sung Kyun Kwan Univ Yakhak Hoeji 38 (3). 1994. 311-321)
4) www.pubmed.gov
Antioxidant/Lipoxygenase Inhibitory Activities and Chemical
Compositions of Selected Essential Oils.(Wei A, Shibamoto T. J Agric
Food Chem. 2010 May 25).
Toxicological evaluation of aqueous extract of Aloe ferox Mill. in
loperamide-induced constipated rats.(Wintola OA, Sunmonu TO,
Afolayan A.Hum Exp Toxicol. 2010 May 24. [Epub ahead of
print]PMID: 20498033
Synthesis of cytotoxic and antioxidant Schiff's base analogs of
aloin.(Kumar S, Matharasi DP, Gopi S, Sivakumar S, Narasimhan S.J
Asian Nat Prod Res. 2010 May;12(5):360-70.PMID: 20496193)
Alternative therapies for common dermatologic disorders, part 2.(Morelli
V, Calmet E, Jhingade V.Prim Care. 2010 Jun;37(2):285-96.PMID:
20493337)
A novel method for air drying aloe leaf slices by covering with filter
papers as a shrink-proof layer.(Kim SA, Baek JH, Lee SJ, Choi SY, Hur
W, Lee SY.J Food Sci. 2009 Nov-Dec; 74(9):E462-70.PMID:
20492108).
Medicinal plants used by traditional medicine practitioners for the
treatment of HIV/AIDS and related conditions in Uganda.(Lamorde M,
Tabuti JR, Obua C, Kukunda-Byobona C, Lanyero H, Byakika-Kibwika
P, Bbosa GS, Lubega A, Ogwal-Okeng J, Ryan M, Waako PJ, Merry C.J
Ethnopharmacol. 2010 May 6. [Epub ahead of print]PMID: 20451595)
Neurotrophins and acupuncture.(Manni L, Albanesi M, Guaragna M,
Barbaro Paparo S, Aloe L.Auton Neurosci. 2010 May 7. [Epub ahead of
print]PMID: 20451467)
Ocular Application of Nerve Growth Factor Protects Degenerating

Retinal Ganglion Cells in a Rat Model of Glaucoma.(Colafrancesco V,
Parisi V, Sposato V, Rossi S, Russo MA, Coassin M, Lambiase A, Aloe
L.J Glaucoma. 2010 Apr 29. [Epub ahead of print]PMID: 20436364).
Protective effects of Aloe vera extract on mitochondria of neuronal cells
and rat brain.(Wang Y, Cao L, Du G.Zhongguo Zhong Yao Za Zhi. 2010
Feb;35(3):364-8. Chinese. PMID: 20423007
An extensive alien plant inventory from the inhabited areas of
galapagos.Guzou A, Trueman M, Buddenhagen CE, Chamorro S,
Guerrero AM, Pozo P, Atkinson R.PLoS One. 2010 Apr
21;5(4):e10276.PMID: 20421999.
Ultrasonic cell grinder extraction of anthraquinones from Radix et
Rhizoma Rhei and determination by ultra-performance liquid
chromatography.Wang GY, Qi HY, Shi YP.J Sep Sci. 2010 Apr 19.
[Epub ahead of print]PMID: 20405483
Emodin, aloe-emodin and rhein induced DNA damage and inhibited
DNA repair gene expression in SCC-4 human tongue cancer cells.Chen
YY, Chiang SY, Lin JG, Yang JS, Ma YS, Liao CL, Lai TY, Tang NY,
Chung JG.Anticancer Res. 2010 Mar;30(3):945-51.PMID: 20393018.
Heavy metal bioaccumulation in selected medicinal plants collected from
Khetri copper mines and comparison with those collected from fertile soil
in Haridwar, India.Maharia RS, Dutta RK, Acharya R, Reddy AV.J
Environ Sci Health B. 2010 Feb;45(2):174-81.PMID: 20390948.
Effects of enhanced UV-B radiation on leaf anthraquinones content and
cell ultrastructure of Aloe vera L.Wang HX, Yang TW, Li JY.Ying Yong
Sheng Tai Xue Bao. 2010 Jan;21(1):260-4. Chinese. PMID: 20387453.
High prevalence of abscesses and self-treatment among injection drug
users in Tijuana, Mexico.Pollini RA, Gallardo M, Hasan S, Minuto J,
Lozada R, Vera A, Ziga ML, Strathdee SA.Int J Infect Dis. 2010 Apr
6. [Epub ahead of print]PMID: 20381396.
Emodin, aloe-emodin and rhein inhibit migration and invasion in human
tongue cancer SCC-4 cells through the inhibition of gene expression of
matrix metalloproteinase-9.Chen YY, Chiang SY, Lin JG, Ma YS, Liao
CL, Weng SW, Lai TY, Chung JG.Int J Oncol. 2010 May;36(5):111320.PMID: 20372784.
Effect of aloe cream versus silver sulfadiazine for healing burn wounds
in rats.Hosseinimehr SJ, Khorasani G, Azadbakht M, Zamani P, Ghasemi
M, Ahmadi A.Acta Dermatovenerol Croat. 2010;18(1):2-7.PMID:
20361881.
8
In vivo evidence of the immunomodulatory activity of orally

administered Aloe vera gel.Im SA, Lee YR, Lee YH, Lee MK, Park YI,
Lee S, Kim K, Lee CK.Arch Pharm Res. 2010 Mar;33(3):451-6. Epub
2010 Mar 30.PMID: 20361311.
Implications for degenerative disorders: Antioxidative activity, total
phenols, flavonoids, ascorbic acid, beta-carotene and beta-tocopherol in
Aloe vera.Ozsoy N, Candoken E, Akev N.Oxid Med Cell Longev. 2009
Apr; 2(2):99-106.PMID: 20357932.
Antifungal activities of Ocimum sanctum essential oil and its lead
molecules.Khan A, Ahmad A, Manzoor N, Khan LA.Nat Prod Commun.
2010 Feb; 5(2):345-9.PMID: 20334156.
VARIOUS PRODUCTS
1) Aloevera Dry Extract- Premium, Standard and EC grade
2) Aloevera Soft Extract- Premium, Standard and EC grade
3) Aloevera Liquid Extract- Premium, Standard and EC grade
4) Aloevera Oil Soluble Extract- Premium, Standard and EC grade
In all we have 12 varieties of Aloevera extracts.
PACKING SIZES/ QUANTITY DETAILS:

Products
Dry Extracts
Soft Extracts
Liquid Extracts
Oil Soluble Extracts
Premium
5kgs
5kgs
5kgs
5kgs
Standard
10kgs
10kgs
10kgs
10kgs
EC
10kgs
10kgs
10kgs
10kgs
Samples are available in 50gms and above to our esteemed clients.

REFERENCES:
1. Indian Herbal Pharmacopoeia (Revised New Edition 2002).
2. Standardization of Botanicals, Volume 2- By Dr. V. Rajpal.
3. www.pubmed.gov
PRODUCT SPECIFICATION
Product Name
Botanical Name
Strength
Part Used
Batch No.
Manufacturing Date
Date of release
TEST
Aloevera Dry Extract - PREMIUM

Aloe indica
6:1
Leaves
KH/AI//0
RESULT
Nature
Color
Taste
Odour
Particle Size
Solubility in Water
Tap Density
PHYSICOCHEMICAL ANALYSIS
Loss on Drying
Water soluble
extractive
Alcohol soluble
extractive
pH (1%w/v Aq.Sol.)
IDENTIFICATION TEST
Identification
PHYTOCHEMICAL ANALYSIS
Aloin
HEAVY METAL ANALYSIS
Arsenic
Lead
Mercury
Cadmium
MYCOTOXIN ANALYSIS
Aflatoxins
(B1+B2+G1+G2)
MICROBIOLOGICAL PROFILE
Total Plate Count
METHOD
STANDARD
REFERENCE
Visual
Visual
Sensory Perception
Smell
NLT 90% (through 80
mesh)
Visual
Mechanical
Fine Powder
Brown
Astringent
Characteristic
80 mesh sieve
ASU Protocol
ASU Protocol
ASU Protocol
ASU Protocol
ASU Protocol
Gravimetric
Maceration
NLT 5%
NLT-80%
API / IP 2007
API / IP 2007
Maceration
--------
API / IP 2007
pH meter
3 to 7
API / IP 2007
TLC
Positive
IP 2007
Spectrophotometric
method
NLT-1.0%
Quality standard of Indian

Medicinal Plant
Soluble
ASU Protocol
0.2 to 0.7gm/ml ASU Protocol
By AAS
By AAS
By AAS
By AAS
NMT-3 ppm
NMT-10 ppm
NMT-1 ppm
NMT-0.3 ppm
By HPLC
0.5+0.1+0.5+0.1 USP (561),ASU Protocol

ppm
Pour Plate Method
NMT500cfu/gm
NMT-102
cfu/gm
Absent
Yeast & Mould
Pour Plate Method
Salmonella sp.
Identification
10
BP 2007,ASU Protocol
USP (61)(62), ASU

Protocol
USP (61)(62), ASU
Protocol
USP (61)(62), ASU
Escherichia. Coli
Pseudomonas
aeroginosa
Staphylococcus
aureus
Total Coliforms
PESTICIDE RESIDUE ANALYSIS
Alachlor
Method
Identification
Method
Identification
Method
Identification
Method
Pour Plate Method
GC Method
Aldrin and dieldrin

(sum of )
Chlorpyrifos
GC Method
DDT(sum of p,pDDT,o,p-DDT,p,p-DDE
and p,p-TDE
Endosulfan(sum of
isomers and Endosulfan
Sulphate)
Ethion
GC Method
GC Method
GC Method
Protocol
USP (61)(62), ASU
Protocol
Absent
USP (61)(62), ASU
Protocol
Absent
USP (61)(62), ASU
Protocol
NMT-10 cfu/gm USP (61)(62), ASU
Protocol
Absent
NMT-0.02
ppm
NMT-0.05
ppm
NMT-0.2
ppm
NMT-1.0
ppm
ASU Protocol, WHO

Guideline
ASU Protocol, WHO
Guideline
ASU Protocol, WHO
Guideline
ASU Protocol, WHO
Guideline
NMT-3.0
ppm
ASU Protocol, WHO

Guideline
GC Method
NMT-2.0
ASU Protocol, WHO
ppm
Guideline
Hexachlorobenzene
GC Method
NMT-0.1
ASU Protocol, WHO
ppm
Guideline
Hexachlorocyclohexane
GC Method
NMT-0.3
ASU Protocol, WHO
isomers(other then a)
ppm
Guideline
Lindane
GC Method
NMT-0.6
ASU Protocol, WHO
ppm
Guideline
Malathion
GC Method
NMT-1.0
ASU Protocol, WHO
ppm
Guideline
STORAGE: Store in air tight containers at around 25C away from direct moisture, heat & light.
SELF LIFE: 2 years from the date of manufactured, if store under recommended conditions.
WARNING: Once the package is opened it should be used at same time or it should be sealed
immediately.
Analysed By
Approved By
11
Product Name
Botanical Name
Strength
Part Used
Batch No.
Manufacturing Date
Date of release
TEST
Aloevera Dry Extract - STANDARD

Aloe indica
3:1
Leaves
KH/AI//
RESULT
METHOD
STANDARD
REFERENCE
PHYSICAL ANALYSIS
Nature
Color
Taste
Odour
Particle Size
Solubility in Water
Tap Density
Loss on Drying
Water soluble
extractive
Alcohol soluble
extractive
pH (1%w/v Aq.Sol.)
IDENTIFICATION TEST
Identification
Aloin
Visual
Visual
Sensory Perception
Smell
NLT 90% (through
80 mesh)
Visual
Mechanical
Fine Powder
Brown
Astringent
Characteristic
80 mesh sieve
ASU Protocol
ASU Protocol
ASU Protocol
ASU Protocol
ASU Protocol
Soluble
0.2 to 0.7gm/ml
ASU Protocol
ASU Protocol
Gravimetric
Maceration
NLT 5%
NLT-80%
API / IP 2007
API / IP 2007
Maceration
--------
API / IP 2007
pH meter
3 to 7
API / IP 2007
TLC
Positive
IP 2007
Spectrophotometric
method
Present

Medicinal Plant
HEAVY METAL ANALYSIS

Arsenic
By AAS
Lead
By AAS
Total Plate Count
Pour Plate Method
Yeast & Mould
Pour Plate Method
Salmonella sp.
Identification
Method
Identification
Method
Identification
Method
Escherichia. Coli
Pseudomonas
aeroginosa
12
NMT-3 ppm
NMT-10 ppm

Protocol
2
Protocol
Absent
USP (61)(62), ASU
Protocol
Absent
USP (61)(62), ASU
Protocol
Absent
USP (61)(62), ASU
Protocol
Staphylococcus
aureus
Total Coliforms
Identification
Method
Pour Plate Method
PESTICIDE RESIDUE ANALYSIS

Alachlor
GC Method
Aldrin and dieldrin
(sum of )
Chlorpyrifos
Absent
NMT-10 cfu/gm
NMT-0.02
ppm
NMT-0.05
ppm
NMT-0.2
ppm
NMT-1.0
ppm
GC Method
GC Method
USP (61)(62), ASU

Protocol
USP (61)(62), ASU
Protocol
ASU Protocol, WHO
Guideline
ASU Protocol, WHO
Guideline
ASU Protocol, WHO
Guideline
ASU Protocol, WHO
Guideline
DDT(sum of p,pGC Method

DDT,o,p-DDT,p,pDDE and p,p-TDE
immediately.
Analysed By
Approved By
13
Product Name
Botanical Name
Strength
Part Used
Batch No.
Manufacturing Date
Date of release
TEST
Aloevera Dry Extract EC grade

Aloe indica
2:1
Leaves
KH/AI//
RESULT
METHOD
STANDARD
REFERENCE
PHYSICAL ANALYSIS
Nature
Color
Taste
Odour
Particle Size
Solubility in Water
Tap Density
Loss on Drying
Water soluble
extractive
Alcohol soluble
extractive
pH (1%w/v Aq.Sol.)
IDENTIFICATION TEST
Identification
Aloin
Visual
Visual
Sensory Perception
Smell
NLT 90% (through
80 mesh)
Visual
Mechanical
Fine Powder
Brown
Astringent
Characteristic
80 mesh sieve
ASU Protocol
ASU Protocol
ASU Protocol
ASU Protocol
ASU Protocol
Soluble
0.2 to 0.7gm/ml
ASU Protocol
ASU Protocol
Gravimetric
Maceration
NLT 5%
NLT-80%
API / IP 2007
API / IP 2007
Maceration
--------
API / IP 2007
pH meter
3 to 7
API / IP 2007
TLC
Positive
IP 2007
Spectrophotometric
method
Present

Medicinal Plant
Total Plate Count
Pour Plate Method

Protocol
2
Yeast & Mould
Pour Plate Method
Protocol
Total Coliforms
Pour Plate Method
Protocol
immediately.
Analysed By
Approved By
14
1. Name of the Product
2. Batch Number
3. Mfg. Date
4. Date of Analysis
5. Part of the Plant Used
6. Ratio
Sr.
No.
A
1.
2.
3.
4.
:Aloe indica, Aloevera Liquid Extract -PREMIUM

: KH/AI//
:
:
: Leaves
: 6:1
Test
Physico chemical
Tests
Color
Odor
Standards
Pale yellow to pale brown

Characteristic
Soluble
Soluble
Soluble
IP-2007
4.0-6.0
IP-2007
1.020-1.050
0.5% - 1.5%
IP-2007
IP-2007
10 ppm
BP-2007 ASU
Protocol
BP-2007 ASU
Protocol
BP-2007 ASU
Protocol
BP-2007 ASU
Protocol
B.
9.
10
Arsenic
3 ppm
11.
Mercury
1 ppm
12.
Cadmium
0.3 ppm
C.
13.
Markers
Total Phenolics
2-50%
6.
7.
8.
14.
D.
15
Ascorbic acid
Mycotoxin
Aflatoxins
(B1+B2+G1+G2)
NMT 0.5+0.1+0.5+0.1
ppm
Pesticide residue
Analysis
Alachlor
NMT 0.02 ppm
16
17
Aldrin and Dieldrin

(Sum of )
Test
Method/Reference
Visual, ASU protocol

Organoleptic, ASU
protocol
IP-2007
IP-2007
Water Solubility
Propylene glycol
Solubility
Alcohol Solubility
(50% alcohol)
pH (1% w/v aqueous
solution)
Specific Gravity (20C)
Dry Residue (% 105C)
Heavy Metals
Lead
5.
Results
1- 100 ppm
NMT 0.05 ppm
15
In house by
colorimetric method
HPLC method
By HPLC, USP
(561),ASU
Protocol
BY GC(ASU
Protocol, WHO
Guideline)
BY GC(ASU
Protocol, WHO
18
Chlorpyrifos
NMT 0.2 ppm
19
NMT 1.0 ppm
21
DDT(Sum Of P,PDDT,O,P-DDT,P,PDDE And P,P-TDE

Endosulfan(Sum Of
Isomers And
Endosulfan Sulphate)
Ethion
22
Hexachlorobenzene
NMT 0.1 ppm
NMT 0.3 ppm
24
Hexachlorocyclohexan
e Isomers(Other then
A)
Lindane
25
Malathion
NMT 1.0 ppm
D.
26.
27.
28.
29.
30.
Microbiological Tests
Total Aerobic Bacteria
Yeast and Moulds
Salmonella sp.
Escherichia. Coli
Pseudomonas
aeroginosa
Staphylococcus aureus
Total Coliforms
20
23
31.
32.
Shelf Life
Storage Conditions
NMT 3.0 ppm
NMT 2.0 ppm
NMT 0.6 ppm
Guideline
BY GC(ASU
Protocol, WHO
Guideline
BY GC(ASU
Protocol, WHO
Guideline
BY GC(ASU
Protocol, WHO
Guideline
BY GC(ASU
Protocol, WHO
Guideline
BY GC(ASU
Protocol, WHO
Guideline
BY GC(ASU
Protocol, WHO
Guideline
BY GC(ASU
Protocol, WHO
Guideline
BY GC(ASU
Protocol, WHO
Guideline
NMT 500 cfu/gm

NMT 100 cfu/gm
Absent
Absent
Absent
ASU Protocol
ASU Protocol
ASU Protocol
ASU Protocol
ASU Protocol
Absent
NMT 10 cfu/gm
ASU Protocol
ASU Protocol
: One year. For use after prescribed period, re-test.

: Cool, Dry and dark clean place. Temperature not exceeding 25oC.
Refrigerate after opening the seal and consume within 15days thereof.
OPINION OF ANALYST
: The above material complies with the prescribed standards.
Analyzed By
Approved By
The values stated above for the product is not valid if, the seal has been tampered or if changes are
seen in the product due to improper handling. Phytochemicals present are likely to degrade over a
period of time and the values stated are as on date of analysis. This is a natural product will darken
with Age, protect from oxidation.
** - Vitamin C, the photochemical analyzed, is a crucial ingredient in any cosmetic preparation,
valued for its antioxidant property. Vitamin C and Phenolics can be used as a marker for obtaining a
high quality standardized product.
16
2. Batch Number
3. Mfg. Date
4. Date of Analysis
6. Ratio
Sr.
No.
A
1.
2.
3.
4.
: Aloe indica, Aloevera Liquid Extract -STANDARD

: KH/AI//
:
:
: Leaves
:4:1
Test
Physico chemical
Tests
Color
Odor
Standards

Characteristic
Soluble
Soluble
Soluble
IP-2007
4.0-6.0
IP-2007
1.020-1.050
0.5% - 1.5%
IP-2007
IP-2007
10 ppm
BP-2007 ASU
Protocol
BP-2007 ASU
Protocol
B.
9.
10
Arsenic
3 ppm
C.
11.
Markers
Ascorbic acid
Present
6.
7.
8.
E
12
Pesticide residue
Analysis
Aldrin and Dieldrin
(Sum of )
Test
Method/Reference

Organoleptic, ASU
protocol
IP-2007
IP-2007
Water Solubility
Propylene glycol
Solubility
Alcohol Solubility
(50% alcohol)
pH (1% w/v aqueous
solution)
Specific Gravity (20C)
Heavy Metals
Lead
5.
Results
NMT 0.05 ppm
13
In house by
Colorimetric method
BY GC(ASU
Protocol, WHO
Guideline
BY GC(ASU
Protocol, WHO
Guideline
DDT(Sum Of P,PNMT 1.0 ppm

DDT,O,P-DDT,P,PDDE And P,P-TDE
D.
14.
NMT 1000 cfu/gm
ASU Protocol
15.
Yeast and Moulds
NMT 100 cfu/gm
ASU Protocol
16.
Total Coliforms
NMT 10 cfu/gm
ASU Protocol
Shelf Life
Storage Conditions
17
OPINION OF ANALYST
Analyzed By
Approved By
18

2. Batch Number
3. Mfg. Date
4. Date of Analysis
6. Ratio
Sr.
No.
A
1.
2.
3.
4.
: Aloe indica ,Aloevera Liquid Extract EC grade

: KH/EO//
:
:
: Fruit
: 4:1
Test
Standards
Physico chemical
Tests
Color
Odor

Characteristic
C.
8.
Water Solubility
Propylene glycol
Solubility
Alcohol Solubility
(50% alcohol)
pH (1% w/v aqueous
solution)
Markers
Ascorbic acid
D.
9.
10.
11.
Yeast and Moulds
Total Coliforms
5.
6.
7.
Shelf Life
Storage Conditions
Results
Test
Method/Reference
Soluble
Soluble

Organoleptic, ASU
protocol
IP-2007
IP-2007
Soluble
IP-2007
4.0-6.0
IP-2007
0.5% - 1.5%
IP-2007
Present
In house by
Colorimetric method
NMT 1000 cfu/gm

NMT 100 cfu/gm
NMT 10 cfu/gm
ASU Protocol
ASU Protocol
ASU Protocol

OPINION OF ANALYST
Analyzed By
Approved By
19

2. Strength
3. Batch Number
4. Mfg. Date
5. Date of Analysis
Sr.
No.
A
1.
Test
: Aloe indica,Aloevera Soft Extract- PREMIUM
: 5:1
: KH/AI/S//
:
:
: Leaves
Results
Physico chemical Tests

Description
Brown colored thick

paste with Characteristic
odour & taste.
Positive
2.
3.
4.
Identification Test by TLC

Water Soluble Extractive as
per IP66
Alcohol Soluble Extractive
as per IP66
5.
6.
B
7.
Standards
Moisture Content
pH (1% w/v aqueous
solution)
Active Ingredient
Aloin((By spectrophotometer
method)
Test
Method/Reference
IP-2007
NLT-80%
IP-2007
---------
IP-2007
NMT 20%
IP-2007
3-7
IP-2007
NLT-0.5%
C
8
Heavy metals
Arsenic
NMT 1 ppm
Lead
NMT 10 ppm
10
Mercury
NMT 1 ppm
11
Cadmium
NMT 0.3 ppm
Quality standard of
Indian Medicinal
Plant
BP-2007 ASU
Protocol
BP-2007 ASU
Protocol
BP-2007 ASU
Protocol
BP-2007 ASU
Protocol
D
12.
13.
17.
15.
16.
17.
18.
Yeast and Moulds
Salmonella sp.
Escherichia. Coli
Pseudomonas aeroginosa
Total Coliforms
NMT 1000 cfu/gm

NMT 100 cfu/gm
Absent
Absent
Absent
Absent
NMT 10 cfu/gm
20
ASU Protocol
ASU Protocol
ASU Protocol
ASU Protocol
ASU Protocol
ASU Protocol
ASU Protocol
immediately.
Analysed By
Approved By
21

2. Strength
3. Batch Number
4. Mfg. Date
5. Date of Analysis
Sr.
No.
A
1.
Test
: Aloe indica, Aloevera Soft Extract- STANDARD
: 3:1
: KH/AI/S//
:
:
: Leaves
Results

Description
4.

per IP66
as per IP66
5.
6.
B
7.
Test
Method/Reference
Brown colored thick

odour & taste.
Positive
2.
3.
Standards
Moisture Content
pH (1% w/v aqueous
solution)
Active Ingredient
method)
IP-2007
NLT-80%
IP-2007
---------
IP-2007
NMT 20%
IP-2007
3-7
IP-2007
Present
C
8
Heavy metals
Arsenic
NMT 1 ppm
Lead
NMT 10 ppm
Quality standard of
Indian Medicinal
Plant
BP-2007 ASU
Protocol
BP-2007 ASU
Protocol
D
10.
11.
12.
13.
NMT 1000 cfu/gm
ASU Protocol
Yeast and Moulds
NMT 100 cfu/gm
ASU Protocol
Absent
ASU Protocol
Total Coliforms
NMT 10 cfu/gm
ASU Protocol
immediately.
Analysed By
Approved By
22
2. Strength
3. Batch Number
4. Mfg. Date
5. Date of Analysis
Sr.
No.
A
1.
Test
: Aloe indica, Aloevera Soft Extract- EC GRADE

: 2:1
: KH/AI/S//
:
:
: Leaves
Results

Description
4.

per IP66
as per IP66
5.
6.
B
7.
Test
Method/Reference
Brown colored thick

odour & taste.
Positive
2.
3.
Standards
Moisture Content
pH (1% w/v aqueous
solution)
Active Ingredient
method)
IP-2007
NLT-80%
IP-2007
---------
IP-2007
NMT 30%
IP-2007
3-7
IP-2007
Present
Quality standard of
Indian Medicinal
Plant
D
8
9.
10.
NMT 1000 cfu/gm
ASU Protocol
Yeast and Moulds
NMT 100 cfu/gm
ASU Protocol
Total Coliforms
NMT 10 cfu/gm
ASU Protocol
immediately.
Analysed By
Approved By
23
Product Name
Botanical Name
Part Used
Ratio
Batch No.
Manufacturing Date
Date of release
TEST
A)PHYSICAL ANALYSIS
1.Appearance
2.Color
Aloevera Oil Soluble PREMIUM

Aloe indica
Leaves
3:1
KH/AI/OS//
RESULT
3.Odour
4.Viscosity
B)PHYSICOCHEMICAL ANALYSIS
5.Specific Gravity ( 20C)
6.Refractive Index
7.Saponification Value
8.Iodine Value
9..Moisture Content
10.Acid Value
11.Rancidity test
12.Peroxide Value
C)HEAVY METAL ANALYSIS
13.Arsenic
14.Lead
15.Mercury
16.Cadmium
D)MICROBIOLOGICAL PROFILE
17.Total Plate Count
18.Yeast & Mould
19.Salmonella sp.
20.Escherichia. Coli
21.Pseudomonas
aeroginosa
22.Total Coliforms
METHOD
Visual
Visual
STANDARD
REFERENCE
ASU Protocol
ASU Protocol
Organoleptic
Viscometer
Transparent Liquid
Light yellow to
light brown
Characteristic
-----
Gravimetric
By Refractometer
Volumetric
Volumetric
Gravimetric
Volumetric
Kries Test
Volumetric
0.70 0.90
1.431 1.489
NMT 0.1
NMT 1%
NMT 0.1 %
NMT 1
Not Rancid
NMT 0.1
ASU Protocol, I.P - 2007

By AAS
By AAS
By AAS
By AAS
NMT 3 ppm
NMT 10 ppm
NMT 1 ppm
NMT 0.3 ppm
Pour Plate Method

Pour Plate Method
Identification
Method
Identification
Method
Identification
Method
Identification
Method
Pour Plate Method
NMT 500cfu/gm
NMT 102 cfu/gm
NMT Absent
USP (61)(62),ASU Protocol

USP (61)(62) ASU Protocol
NMT Absent
NMT Absent
NMT Absent
NMT 10 cfu/gm
ASU Protocol
SELF LIFE: One year. For use after Prescribed, re-test.
OPINION OF ANALYST: The above material complies with the prescribed standards.
Analyzed By
Approved By
24
Product Name
Botanical Name
Part Used
Ratio
Batch No.
Manufacturing Date
Date of release
TEST
A)PHYSICAL ANALYSIS
1.Appearance
2.Color
Aloevera Oil Soluble STANDARD

Aloe indica
Leaves
2:1
KH/AI/OS//
RESULT
3.Odour
4.Viscosity
6.Refractive Index
7.Saponification Value
8.Iodine Value
9..Moisture Content
10.Acid Value
C)HEAVY METAL ANALYSIS
11.Arsenic
12.Lead
13.Total Plate Count
14.Yeast & Mould
15.Total Coliforms
METHOD
Visual
Visual
STANDARD
REFERENCE
ASU Protocol
ASU Protocol
Organoleptic
Viscometer
Transparent Liquid
Light yellow to
light brown
Characteristic
-----
Gravimetric
By Refractometer
Volumetric
Volumetric
Gravimetric
Volumetric
0.70 0.90
1.431 1.489
NMT 0.1
NMT 1%
NMT 0.1 %
NMT 1
ASU Protocol, I.P 2007

By AAS
By AAS
NMT 3 ppm
NMT 10 ppm
Pour Plate Method

Pour Plate Method
Pour Plate Method
NMT 103 cfu/gm

NMT 102 cfu/gm
NMT 10 cfu/gm

ASU Protocol
Analyzed By
Approved By
25
Product Name
Botanical Name
Part Used
Ratio
Batch No.
Manufacturing Date
Date of release
TEST
A)PHYSICAL ANALYSIS
1.Appearance
2.Color
Aloevera Oil Soluble EC GRADE

Aloe indica
Leaves
1:1
KH/AI/OS//
RESULT
3.Odour
4.Viscosity
6.Refractive Index
7..Moisture Content
8.Total Plate Count
9.Yeast & Mould
10.Total Coliforms
METHOD
Visual
Visual
STANDARD
REFERENCE
ASU Protocol
ASU Protocol
Organoleptic
Viscometer
Transparent Liquid
Light yellow to
light brown
Characteristic
-----
Gravimetric
By Refractometer
Gravimetric
0.70 0.90
1.431 1.489
NMT 0.1 %

Pour Plate Method

Pour Plate Method
Pour Plate Method
NMT 103 cfu/gm

NMT 102 cfu/gm
NMT 10 cfu/gm

ASU Protocol
Analyzed By
Approved By
26

Aloe Vera

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HERBAL MONOGRAPH OF ALOE BARBADENSIS

(syn. ALOE INDICA)

Kumarirasasambhava, Ghrita- Kumari

IDENTITY TESTS (FROM RAW MATERIALS)

2) Aloin estimations by Gravimetric method.

independent of the number of viable molds that are detected using

a) J Wound Care. 2004 Apr;13(4):157-8.: Phytotherapy: an alternative

Ocular Application of Nerve Growth Factor Protects Degenerating

In vivo evidence of the immunomodulatory activity of orally

PACKING SIZES/ QUANTITY DETAILS:

Samples are available in 50gms and above to our esteemed clients.

Aloevera Dry Extract - PREMIUM

Quality standard of Indian

0.5+0.1+0.5+0.1 USP (561),ASU Protocol

Pour Plate Method

Yeast & Mould

Pour Plate Method

USP (61)(62), ASU

Aldrin and dieldrin

ASU Protocol, WHO

ASU Protocol, WHO

Aloevera Dry Extract - STANDARD

Quality standard of Indian

HEAVY METAL ANALYSIS

Pour Plate Method

NMT-103 cfu/gm USP (61)(62), ASU

PESTICIDE RESIDUE ANALYSIS

USP (61)(62), ASU

DDT(sum of p,pGC Method

Aloevera Dry Extract EC grade

Quality standard of Indian

NMT-103 cfu/gm USP (61)(62), ASU

:Aloe indica, Aloevera Liquid Extract -PREMIUM

Pale yellow to pale brown

NMT 0.02 ppm

Aldrin and Dieldrin

Visual, ASU protocol

NMT 0.05 ppm

NMT 0.2 ppm

NMT 1.0 ppm

DDT(Sum Of P,PDDT,O,P-DDT,P,PDDE And P,P-TDE

NMT 0.1 ppm

NMT 0.3 ppm

NMT 1.0 ppm

NMT 3.0 ppm

NMT 2.0 ppm

NMT 0.6 ppm

NMT 500 cfu/gm

: One year. For use after prescribed period, re-test.

: The above material complies with the prescribed standards.

: Aloe indica, Aloevera Liquid Extract -STANDARD

Pale yellow to pale brown

Visual, ASU protocol

NMT 0.05 ppm

DDT(Sum Of P,PNMT 1.0 ppm

: The above material complies with the prescribed standards.

1. Name of the Product

: Aloe indica ,Aloevera Liquid Extract EC grade

Pale yellow to pale brown

Visual, ASU protocol

NMT 1000 cfu/gm

: One year. For use after prescribed period, re-test.

: The above material complies with the prescribed standards.