JCY HDD TECHNOLOGY SDN.

BHD
DOCUMENT REVISION SUMMARY
Title:LIQUID PARTICLE COUNT (LPC-S02)

Page No:

Current Revision

Date

1 of 1

09

10 May 2014

Prepared By :

KHOO WEI LIANG

OGY SDN.BHD

N SUMMARY
Doc No : CM-LAB-009

Remark

Approved By :

DOC. NO

CM-LAB- 009
KHOO WEI LIANG

PREPARED BY
ROSHAZAIMI
APPROVED BY
REF. DOC. NO

92-004230 Rev AK

JCY HDD TECHNOLOGY SDN BHD

WORK INSTRUCTION (WD)
REVISION
10
PRODUCT
GENERAL
DEPARTMENT

ANALYTICAL LAB

TEST

LIQUID PARTICLE COUNT (LPC-S02) - W

DATE

10-May-14

PAGE

1 of 1

1. TEST SET-UP / PREPARATION

1.1 Change water inside ultrasonic tank by:
1.1.1 Drain out water from ultrasonic tank.
1.1.2 Wipe the ultrasonic tank with IPA.
1.1.3 Fill-in the ultrasonic tank with DI water, 18.2 L volume.
1.2 Degas the water in ultrasonic tank by:
1.2.1 Pushing the switch behind the generator to "low" (degas mode).
1.2.2 Turning on the power to 100% of full power for 10 min.
1.2.3 Return the switch to "HI" (extraction mode) for LPC extraction.
1.3 Measure the Dissolved Oxigen (DO) level and temperature by placing the DO meter in a clean
beaker with supply water overflowing. The clean beaker is close to the outlet point of water tube.
The DO level must be in 40 + 2% at 25 + 5oC.
1.4 Set input power of the generator, 97watt for LPC extraction.
1.5 Measure the power delivered into water in 2000ml beaker by using Hydrophone. The hydrophone
measurement requirement is 2.900 + 0.2V AC. Refer appendix XXX for Hydrophone measurement
method.
1.6 Monitor / record the DO level and temperature of water, hydrophone measurement and DC light
value. These parameter and water level in tank must be maintained throughout analysis.
Note: DC light value should not exceed 0.5, perform sensor cleaning as per instruction from
manufacturer.
2. TEST PROCEDURE
2.1 Blank Determination
2.1.1 Fill 2000ml of DI water into beaker.
2.1.2 Place the beaker filled with extraction water into the ultrasonic bath.
2.1.3 Lower appropriate fishing line fixture into the beaker and ensure it is equal to the level used
in sample determination
2.1.4 Start the timer and sonicate for 2 minutes 2 seconds
2.1.5 After 2 minutes, turn off the ultrasonic power and lift the fishing line fixture. Take out the
beaker from ultrasonic tank and carefully transfer the beaker to the LPC stir base.
Place the sample capillary at the center of beaker.
2.1.6 Put the cleaned stir bar into the beaker by using magnetic stir bar retriever to pull and

move down slowly.

2.1.7 Turn the stir bar rate to 75-150 rpm and without creating large vortex for 1 minute before
sampling to allow constant mixing.
2.1.8 Then, perform Blank measurement during stirring and using parameter in appendix XXX.
2.1.9 If the particles count >20 particle/ml, clean the beaker and repeat steps 2.1.1 to 2.1.8.
2.1.10 If an acceptable blank cannot achieve within 2 hours, check the following: the beaker,
fishing line, filters on DI water source and HEPA filters of the laminar flow hood.
2.1.11 Remove the stir bar by using magnetic stir bar retriever to pull out and submerge it into
DI water.
2.1.12 Add the water, 40ml into the beaker for sample extraction.
2.2 Sample Determination
2.2.1 Place the beaker from section 2.2.12 in the ultrasonic tank.
2.2.2 Using clean forcep or tweezer (DI rinsed), place the sample on the fishing line fixture.
Do not touch the sample with anything unless using forcep or tweezer.
2.2.3 Lower the fishing line fixture into the beaker and ensure the sample is horizontally in the
center of the extraction beaker and the top of the sample is vertically approximately 1/2'' from
top surface of water.
Note: Ensure the entire sample is fully submerge into water and not touching the beaker.
2.2.4 Repeat step d to h in Section 1.
f) Calculate the result based on calculation given below.
SKETCH/REMARKS
RESULT CALCULATION (SEAGATE).

Total Number of Particles/cm2 =

where:
S = Sample counts (counts/ mL)
B = Blanks counts (counts/ mL)
V = Extraction volume (mL)
N = Number of parts extracted
A = surface area of part used (sq. cm)

Fig. 1
Ultrasonic Extraction Conditions:
Component
Base
Cover
Antidisk

Vol of Extraction Water

2000mL
2000mL
2000 mL

Vol of Water in Tank

18 L
18 L
18 L

Fig. 2

OUNT (LPC-S02) - WD METHOD

in a clean
water tube.

hydrophone
measurement

nd DC light

he level used

ute before

endix XXX.

rge it into

ally in the
ately 1/2'' from

ker.

Fig. 2

Fig. 3

DOC. NO

CM-LAB- 009
KHOO WEI LIANG

PREPARED BY
SRI RAHAYU
APPROVED BY
REFERENCE DOC NO

92-004230 Rev AK

JCY HDD TECHNOLOGY SDN BHD

WORK INSTRUCTION (WD)
REVISION
10
PRODUCT
GENERAL
DEPARTMENT

ANALYTICAL LAB

TEST

LIQUID PARTICLE COUNT (LPC-S02) -

DATE

10-May-14

PAGE

1 OF 1

METHOD

1.Measure and check water supply (DI water) :

a) Before testing, make sure to measure the DI water supply temperature at 25 5C and the Dissolved Oxygen level within 40 2
degassifier and Handy Polaris Oxyguard DO meter.
b) Put DO meter into the beaker and fill the DO water until overflow. Start timer to record the time taken to obtain 40 2% DO leve
( approximately 2 minutes). Record the time and use this time during sample extraction.
2.Conditioned the ultrasonic tank before use :

a) Fill Ultrasonic tank with volume of DO water according to volume of water extraction. Make sure water level in tank same as wate
b) DEGAS the water in ultrasonic tank by turning on the power to 100% of full power for 10 min.
3. Blank Determination
a) Set the ultrasonic power (2.900 0.2 V AC for 2L beaker/ 3.100 0.2 V AC) using a WD approved hydrophone.
b) Then place the beaker filled with extraction water into the ultrasonic bath.
c) Lower appropriate fishing line fixture into the beaker and ensure it is equal to the level used in sample determination
d) Start the timer and sonicate for 2 minutes 2 seconds
e) Transfer the beaker to the LPC stir base.
f) Put the stir bar into the beaker by using retriever to pull and move down slowly.
g) Turn the stir bar rate to 75-150rpm for 1 minute.
h) Place the sample capillary into the beaker at the center and measure the sample counts.

Note: DC light value should not exceed 0.5, discard the blank and perform sensor cleaning as per instruction from manufacturer.
4. Sample Determination

a) Turn on ultrasonic tank and set input power.

b) Place the beaker from section 1 in the ultrasonic bath
c) Place the part(s) on the fishing line fixture by using clean forceps or tweezers.

d) Lower the fishing line fixture into the beaker and ensure that the part is horizontally in the center of the extraction beaker and th
1/2'' from surface water.
e) Repeat step d to h in Section 1.
f) Calculate the result based on calculation given below.
SKETCH/REMARKS
RESULT CALCULATION (SEAGATE).

Total Number of Particles/cm2 =

where:
S = Sample counts (counts/ mL)
B = Blanks counts (counts/ mL)
V = Extraction volume (mL)
N = Number of parts extracted
A = surface area of part used (sq. cm)

Fig. 1
Ultrasonic Extraction Conditions:
Component
Base
Cover
Antidisk

Vol of Extraction Water

2000mL
2000mL
2000 mL

Vol of Water in Tank

18 L
18 L
18 L

Fig. 2

JCY HDD TECHNOLOGY SDN BHD

WORK INSTRUCTION (WD)
10
GENERAL
ANALYTICAL LAB
LIQUID PARTICLE COUNT (LPC-S02) - WD METHOD
10-May-14
1 OF 1

METHOD

supply temperature at 25 5C and the Dissolved Oxygen level within 40 2% by using

until overflow. Start timer to record the time taken to obtain 40 2% DO level water
his time during sample extraction.

ng to volume of water extraction. Make sure water level in tank same as water level in beaker.

eaker/ 3.100 0.2 V AC) using a WD approved hydrophone.

r and ensure it is equal to the level used in sample determination

nter and measure the sample counts.

e blank and perform sensor cleaning as per instruction from manufacturer.

sure that the part is horizontally in the center of the extraction beaker and the top part is vertically

w.
SKETCH/REMARKS

Fig. 2

Fig. 3