1.

APPARATUS AND MATERIALS

1.5.1 APPARATUS

60mL BOD bottle with stopper

300mL BOD bottle with stopper
Measuring cylinder
Graduated cylinder
250mL Erlenmeyer tube
Digital titrator

BOD bottle with stopper

titrator

1.5.2 MATERIALS

Water sample
Manganous Sulfate Powder Pillow
Alkaline Iodide-Azide Reagent Powder Pillow
Sulfamic acid powder Pliiow
Sodium Thiosulfate Titration Catridge
2mL of starch
Dissolved Oxygen 1 Reagent Powder Pillow
Dissolved Oxygen 2 Reagent Powder Pillow
Dissolved Oxygen 3 Reagent Powder Pillow

Digital

1.6 METHADOLOGY / PROCEDURES

1.6.1 USING A 60 ML BOD BOTTLE

1. A water sample is collected in a clean 60mL glass stoppered BOD
bottle.
2. The content of one Dissolved Oxygen 1 Reagent Powder Pillow
and one Dissolved Oxygen 2 Reagent Powder Pillow are added.
3. The stopper is immediately inserted so air is not trapped in the
bottle. The bottle is inverted several times to mix.
4. Waited until the floc in the solution has settled and the top half of
the solution is clear. The bottle is again inverted several times
and waited until the floc has settled.
5. The stopper is removed and the content of one Dissolved Oxygen
3 Powder Pillow is added. The stopper is replaced without
trapping air in the bottle and inverted several times to mix.
6. 20mL of the prepared sample is accurately measured and been
transferred into a 250mL Erlenmeyer flask.
7. A clean straight-stem delivery tube is attached to a 0.2000N
Sodium Thiosulfate Titration Catridge. The catridge is twisted
onto the titrator body.
8. The delivery tube is flushed by turning the delivery knob to eject
a few drops of titrant. The conter is reset to zero and the tip is
wiped.
9. The prepared soluyion is titrated with 0.2000N Sodium
Thiosulfate until the sample changes from yellow-to colourless.
The number of digits is recorded.
10.
The dissolved oxygen is calculated.

1.6.2 USING A 300ML BOD BOTTLE

1. A water sample is collected in a clean 300mL BOD bottle.
2. The contents of one Manganous Sulfate Powder Pillow and one
Alkaline Iodide-Azide Reagent Powder Pillow are added.
3. The stopper is immediately inserted so air is not trapped in the
bottle. Inverted several times to mix.
4. When the floc in the solution has settled, again the bottle is
inverted again several times until the floc has been settled.

5. The stopper is removed and the contents of one Sulfamic Acid

Powder Pillow is added. The stopper is replaced without trapping
air in the bottle and the prepared sample is inverted several
times to mix.
6. A sample volume and Sodium Thiosulfate Titration Catridge is
selected corresponding to the expected dissolved oxygen ( D.O. )
concentration from Table 1.
7. A clean delivery tube is inserted into the titration catridge. The
catridge is attached to the titrator body.
8. The delivery knob is turned to eject a few drops of titrant. The
counter is reset to zero and the tip is wiped.
9. A graduated cylinder is used to measure the sample volume from
Table 1. The sample is transferred into a 250mL Erlenmeyer flask.
10.
The delivery tube tip is placed into the solution and the
flask is swirled with titrating with sodium thiosulfate to a pale
yellow colour.
11.
Two 1mL of Starch Indicator Solution is added using a
dropper and swirled to mix.
12.
The titration is continued to a colorless end point. The
number of digits required is recorded.
13.
The dissolved oxygen is calculated.

1.10 CONCLUSION
The digit required using 60mL water sample is 87 so the dissolved
oxygen is 8.7 mg/L. For the experiment using 100 mL water sample in 300mL
BOD bottle, the digit required is 410 so the dissolved oxygen is 8.2 mg/L.
Both resulting dissolved oxygen are in range 2-10 so the experiment is
considered successful.
1.11 RECOMMENDATIONS
1. Make sure there is no air bubbles after water sample has been
poured into BOD bottle to avoid aeration.
2. Conduct the experiment immediately after the water sample is
taken from the field to ensure the dissolved oxygen in water
sample is not mixed with dissolved oxygen in the surrounding.
3. Ensure the is no trapped air at the tip of the titrator so that the
volume of sodium thiosulfate is accurate.

4. The solubility of oxygen in water is greater at cooler

temperature, so that it is more efficient to conduct the
experiment at low temperature place.
5. Make sure the water sample containers must be clean, dry and
free of contaminants. The best method to prepare water sample
containers is by acid cleaning with dichromate acid.