Documente Academic
Documente Profesional
Documente Cultură
Original Article
Abstract
Objective: Evolution, inappropriate and over use of antibiotics is responsible for antibiotics and drugs resistance among the microbes. Drug resistance
microbes can increase the chances of death, complications of illness, requires prolonged treatment and more hospital stays. This situation makes
immune-compromised patients more prone to nosocomial infections. Mother Nature has been inexhaustible and infinite source for medicinal
compounds. Asian communities are using spices, cow urine and medicinal plants extracts in their diets and naturopathy.
Methods: Antimicrobial activities of photo activated Cow Urine, Azadirachta indica, Terminalia chebula, and Piper nigrum were tested on the basis
of Minimum Inhibitory Concentration which was tested singularly as well as with all possible combinations by agar diffusion and broth dilution
methods against Streptomyces aureofaciens (MTCC 325), E.coli (MTCC 448), Streptococcus mutans (MTCC 497), Pseudomonas aeruginosa (MTCC
7093) Candida albicans (MTCC183), Candida paraopsilosis (MTCC 1965), Candida tropicalis (MTCC 184) and Candida glabrata (MTCC 3019).
Results: Motivating results were obtained. Azadirachta indica, Piper nigrum, terminalia chebula and Cow urine has shown remarkable antimicrobial
effects. On an average MIC for cow urine, Azadirachta indica Piper nigrum, terminalia chebula was 200l, 70-100l, 100l and 90l respectively. T.chebula
showed superb antimicrobial activity against C. propsiolosis, S. aureofaciens, S. mutans and C. albicans. It also has moderate antimicrobial activity
against P. aeruginosa, E.coli, C. tropicalis and C. glabrata. A. indica was antimicrobial against S. aerufaciens, C. albicans, E. coli, P. aeruginosa and C.
glabrata. Cow urine was antimicrobial agains E.coli, C. albicans and S. aeruginosa. Piper nigrum was antimicrobial against S. aerufaciens,
C.tropicalis and C. propsiolosis. Remarkable synergistic effects were observed where cow urine and T. chebula, A. indica and cow urine, T. chebula and
Piper nigrum, cow urine and Piper nigrum, A. indica and Piper nigrum, A. indica and T. chebula, and also when used in a combination of three and four
and the antimicrobicity against all microbes got enhanced.
Conclusion: The results from this study suggest the effects of these natural agents against pathogenic microbes were impressive but depend upon the
combination used and tested against which microbes. Overall the synergistic and singular use inhibited the growth of microbes. Results from this study
reconfirms and strengthen our position to naturally tackle the challenge of disease caused by pathogenic microbes and can be helpful in finding a
solution to outdating of antibiotics and decreases our dependence on them.
INTRODUCTION
e ISSN: 2231-2218
2012 Asian J Pharm Biol Res
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CONCLUSION
Results reported from this one of kind study confirm that
nature is the best healer. Synergistics effects resulting from the
combinations of Terminalia chebula, Piper nigrum, Azadirachta
indica and of cow urine have never been documented earlier.
Combined effect of natural substances i.e. drug synergism is a
novel concept. Azadirachta indica was found to be most effective
against S aureofaciens, Terminalia chebula against C. propsilosis,
cow urine against C. albicans and Piper nigrum against C.
tropicalis. Phytochemicals from T. chebula, Piper nigrum, A.
indica and cow urine in different combinations can be of great
significance in therapeutic treatments. Antimicrobial agents
present in these Indian spices, medicinal plants and cow urine can
be effective against plants, human and animal pathogens.
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